{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002345","ANALYSIS_ID":"AN003829","VERSION":"1","CREATED_ON":"12-15-2022"},

"PROJECT":{"PROJECT_TITLE":"Stress-Induced Mucosal Layer Disruption Drives Gut Dysbiosis and Depressive-like Behaviors","PROJECT_SUMMARY":"Depression is a common mental health condition with a large social and economic impact. While depression etiology is multifactorial, chronic stress is a well-accepted contributor to disease onset. In addition, depression is associated with altered gut microbial signatures that can be replicated in animal models. While targeted restoration of the microbiome has been shown to reduce depressive-like behaviors in mice, the complexity and diversity of the human microbiome has complicated therapeutic intervention in patients. To circumvent these limitations, there is a critical need for identifying pathways responsible for microbiome dysbiosis. Here, for the first time, we identify the changes in host physiology that induce microbiome dysbiosis. Specifically, we show that a component of mucosal layer, the transmembrane protein mucin 13, can regulate microbiome composition. Using a model of chronic stress to induce behavioral and microbial changes in mice, we show a significant reduction in mucin 13 expression across the intestines that occurs independently of the microbiome. Furthermore, deleting Muc13 leads to gut dysbiosis, and baseline behavioral changes normally observed after stress exposure. Together, these results validate the hypothesis that mucosal layer disruption is an initiating event in stress-induced dysbiosis and offer mucin 13 as a potential new therapeutic target for microbiome dysbiosis in stress-induced depression. For the first time, our data provide an upstream and conserved target for treating microbiome dysbiosis, a result with sweeping implications for diseases presenting with microbial alterations.","INSTITUTE":"University of Virginia","DEPARTMENT":"Neuroscience","LABORATORY":"Gaultier Lab","LAST_NAME":"Rivet-Noor","FIRST_NAME":"Courtney","ADDRESS":"409 Lane Road, Charlottsville, Virginia, 22903, USA","EMAIL":"crr4tz@virginia.edu","PHONE":"434-243-1903","FUNDING_SOURCE":"NIH","DOI":"http://dx.doi.org/10.21228/M85717"},

"STUDY":{"STUDY_TITLE":"Stress-Induced Mucosal Layer Disruption Drives Gut Dysbiosis and Depressive-like Behaviors","STUDY_SUMMARY":"Depression is a common mental health condition with a large social and economic impact. While depression etiology is multifactorial, chronic stress is a well-accepted contributor to disease onset. In addition, depression is associated with altered gut microbial signatures that can be replicated in animal models. While targeted restoration of the microbiome has been shown to reduce depressive-like behaviors in mice, the complexity and diversity of the human microbiome has complicated therapeutic intervention in patients. To circumvent these limitations, there is a critical need for identifying pathways responsible for microbiome dysbiosis. Here, for the first time, we identify the changes in host physiology that induce microbiome dysbiosis. Specifically, we show that a component of mucosal layer, the transmembrane protein mucin 13, can regulate microbiome composition. Using a model of chronic stress to induce behavioral and microbial changes in mice, we show a significant reduction in mucin 13 expression across the intestines that occurs independently of the microbiome. Furthermore, deleting Muc13 leads to gut dysbiosis, and baseline behavioral changes normally observed after stress exposure. Together, these results validate the hypothesis that mucosal layer disruption is an initiating event in stress-induced dysbiosis and offer mucin 13 as a potential new therapeutic target for microbiome dysbiosis in stress-induced depression. For the first time, our data provide an upstream and conserved target for treating microbiome dysbiosis, a result with sweeping implications for diseases presenting with microbial alterations.","INSTITUTE":"University of Virginia","LAST_NAME":"Rivet-Noor","FIRST_NAME":"Courtney","ADDRESS":"409 Lane Road, Charlottsville, Virginia, 22903, USA","EMAIL":"crr4tz@virginia.edu","PHONE":"434-243-1903","SUBMIT_DATE":"2022-11-10"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","AGE_OR_AGE_RANGE":"12-24 weeks","GENDER":"Male","ANIMAL_ANIMAL_SUPPLIER":"Jackson","ANIMAL_LIGHT_CYCLE":"12L/12D"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"blank1",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_blank1"}
},
{
"Subject ID":"-",
"Sample ID":"blank2",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_blank2"}
},
{
"Subject ID":"-",
"Sample ID":"blank3",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_blank3"}
},
{
"Subject ID":"-",
"Sample ID":"blank4",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_blank4"}
},
{
"Subject ID":"-",
"Sample ID":"Cal1_A",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Cal1_A"}
},
{
"Subject ID":"-",
"Sample ID":"Cal1_B",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Cal1_B"}
},
{
"Subject ID":"-",
"Sample ID":"Cal2_A",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Cal2_A"}
},
{
"Subject ID":"-",
"Sample ID":"Cal2_B",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Cal2_B"}
},
{
"Subject ID":"-",
"Sample ID":"Cal3_A",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Cal3_A"}
},
{
"Subject ID":"-",
"Sample ID":"Cal3_B",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Cal3_B"}
},
{
"Subject ID":"-",
"Sample ID":"Cal4_A",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Cal4_A"}
},
{
"Subject ID":"-",
"Sample ID":"Cal4_B",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Cal4_B"}
},
{
"Subject ID":"-",
"Sample ID":"Cal5_A",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Cal5_A"}
},
{
"Subject ID":"-",
"Sample ID":"Cal5_B",
"Factors":{"Group":"CTL"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Cal5_B"}
},
{
"Subject ID":"-",
"Sample ID":"Naive_226",
"Factors":{"Group":"Naïve"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Naive_226"}
},
{
"Subject ID":"-",
"Sample ID":"Naive_227",
"Factors":{"Group":"Naïve"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Naive_227"}
},
{
"Subject ID":"-",
"Sample ID":"Naive_229",
"Factors":{"Group":"Naïve"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Naive_229"}
},
{
"Subject ID":"-",
"Sample ID":"Naive_230",
"Factors":{"Group":"Naïve"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Naive_230"}
},
{
"Subject ID":"-",
"Sample ID":"Naive_231",
"Factors":{"Group":"Naïve"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Naive_231"}
},
{
"Subject ID":"-",
"Sample ID":"Naive_237",
"Factors":{"Group":"Naïve"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Naive_237"}
},
{
"Subject ID":"-",
"Sample ID":"Naive_238",
"Factors":{"Group":"Naïve"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Naive_238"}
},
{
"Subject ID":"-",
"Sample ID":"Naive_239",
"Factors":{"Group":"Naïve"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Naive_239"}
},
{
"Subject ID":"-",
"Sample ID":"Naive_247",
"Factors":{"Group":"Naïve"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Naive_247"}
},
{
"Subject ID":"-",
"Sample ID":"Naive_248",
"Factors":{"Group":"Naïve"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Naive_248"}
},
{
"Subject ID":"-",
"Sample ID":"Naive_249",
"Factors":{"Group":"Naïve"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Naive_249"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_232",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_232"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_233",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_233"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_234",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_234"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_235",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_235"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_236",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_236"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_240",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_240"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_241",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_241"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_242",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_242"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_243",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_243"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_244",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_244"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_245",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_245"}
},
{
"Subject ID":"-",
"Sample ID":"Stress_246",
"Factors":{"Group":"Stress"},
"Additional sample data":{"RAW_FILE_NAME":"x06242021x_PRM_Stress_246"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Whole blood was extracted from animals at the time of euthanization from the heart chamber. Blood was collected into blood collection tubes (Fisher Scientific; #02-675-185) and spun for 10 min at 11,000g. Serum was collected and frozen in liquid nitrogen. 25uL of plasma was extracted with 500uL of acetonitrile by vortexing and centrifugation at 10min at 14,000rpm. 450uL of supernatant was transferred to new tube and dried via SpeedVac. Dried samples were reconstituted with 25uL of 50% methanol and transferred to autosampler vials. Injection volume =10uL in PRM mode for detection and quantification of 10 different analytes. Metabolite mixture was analyzed on Thermo Orbitrap IDX MS system coupled to a Vanquish UPLC system. Samples were transported via the autosampler (10uL injection volume) onto a Waters BEH C18 column. Runtime was 15min in PRM mode. Buffer A: 0.1% formic acid in water. Buffer B: 0.1% formic acid in methanol. LC Gradient: 0min: 0% B, 8min: 50% B, 9 min: 98% B, 13min: 98% B. Recalibration of system up to 15 min at 0% B for next injection.","SAMPLE_TYPE":"Blood (serum)","COLLECTION_METHOD":"Cardiac Puncture","COLLECTION_LOCATION":"Heart","STORAGE_CONDITIONS":"Described in summary"},

"TREATMENT":{"TREATMENT_SUMMARY":"Mice were subjected to 3weeks of unpredictable chronic mild restraint stress or kept in a naive setting"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Whole blood was extracted from animals at the time of euthanization from the heart chamber. Blood was collected into blood collection tubes (Fisher Scientific; #02-675-185) and spun for 10 min at 11,000g. Serum was collected and frozen in liquid nitrogen. 25uL of plasma was extracted with 500uL of acetonitrile by vortexing and centrifugation at 10min at 14,000rpm. 450uL of supernatant was transferred to new tube and dried via SpeedVac. Dried samples were reconstituted with 25uL of 50% methanol and transferred to autosampler vials. Injection volume =10uL in PRM mode for detection and quantification of 10 different analytes. Metabolite mixture was analyzed on Thermo Orbitrap IDX MS system coupled to a Vanquish UPLC system. Samples were transported via the autosampler (10uL injection volume) onto a Waters BEH C18 column. Runtime was 15min in PRM mode. Buffer A: 0.1% formic acid in water. Buffer B: 0.1% formic acid in methanol. LC Gradient: 0min: 0% B, 8min: 50% B, 9 min: 98% B, 13min: 98% B. Recalibration of system up to 15 min at 0% B for next injection.","PROCESSING_STORAGE_CONDITIONS":"Described in summary","EXTRACT_STORAGE":"Described in summary"},

"CHROMATOGRAPHY":{"INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Waters Acquity BEH C18 (100 x 2mm,1.7um)","FLOW_GRADIENT":"0min: 0% B, 8min: 50% B, 9 min: 98% B, 13min: 98% B. Recalibration of system up to 15 min at 0% B for next injection.","SOLVENT_A":"100% water; 0.1% formic acid","SOLVENT_B":"100% methanol 0.1% formic acid","ANALYTICAL_TIME":"15 min","CHROMATOGRAPHY_TYPE":"Reversed phase"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Orbitrap ID-X tribrid","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"Other","MS_COMMENTS":"Raw data files were brought into Skyline software. Targeted peak detection was done based on the parent mass. Mass analyzer set to Orbitrap and resolution power set to 120,000 resolution. Then all raw files and unknown samples were imported to Skyline. Calibration curves were generated by Linear regression fit. Targeted precursor MZ and MZ of analytes was used to track and quantification of the metabolite. Peak areas for analytes in samples were used for quantification based in the generated calibration curves.","ION_MODE":"UNSPECIFIED"},

"MS_METABOLITE_DATA":{
"Units":"ug/mL",

"Data":[{"Metabolite":"Aldosterone","Naive_226":"0.0006","Naive_227":"0.0009","Naive_229":"","Naive_230":"","Naive_231":"0.0005","Naive_237":"0.0012","Naive_238":"0.0003","Naive_239":"","Naive_247":"0.0006","Naive_248":"","Naive_249":"0.0010","Stress_232":"0.0029","Stress_233":"0.0004","Stress_234":"0.0021","Stress_235":"0.0006","Stress_236":"0.0004","Stress_240":"0.0005","Stress_241":"0.0019","Stress_242":"0.0006","Stress_243":"0.0024","Stress_244":"0.0010","Stress_245":"0.0008","Stress_246":"0.0006"},{"Metabolite":"corticosterone","Naive_226":"0.2340","Naive_227":"0.1613","Naive_229":"0.0866","Naive_230":"0.2250","Naive_231":"0.2597","Naive_237":"0.0983","Naive_238":"0.1150","Naive_239":"0.0872","Naive_247":"0.1040","Naive_248":"0.1956","Naive_249":"0.0175","Stress_232":"0.1676","Stress_233":"0.0940","Stress_234":"0.1090","Stress_235":"0.0855","Stress_236":"0.1096","Stress_240":"0.0465","Stress_241":"0.0681","Stress_242":"0.3389","Stress_243":"0.0615","Stress_244":"0.1631","Stress_245":"0.1515","Stress_246":"0.1038"},{"Metabolite":"cortisol","Naive_226":"0.0005","Naive_227":"","Naive_229":"","Naive_230":"0.0007","Naive_231":"0.0006","Naive_237":"","Naive_238":"","Naive_239":"","Naive_247":"0.0009","Naive_248":"0.0028","Naive_249":"","Stress_232":"0.0022","Stress_233":"0.0009","Stress_234":"","Stress_235":"0.0017","Stress_236":"0.0039","Stress_240":"0.0020","Stress_241":"0.0044","Stress_242":"0.0177","Stress_243":"0.0094","Stress_244":"0.0067","Stress_245":"0.0119","Stress_246":"0.0053"},{"Metabolite":"Dopamine","Naive_226":"0.0001","Naive_227":"0.0004","Naive_229":"0.0004","Naive_230":"0.0001","Naive_231":"","Naive_237":"0.0004","Naive_238":"0.0003","Naive_239":"0.0002","Naive_247":"0.0001","Naive_248":"0.0001","Naive_249":"","Stress_232":"0.0002","Stress_233":"0.0003","Stress_234":"","Stress_235":"","Stress_236":"","Stress_240":"","Stress_241":"0.0008","Stress_242":"0.0002","Stress_243":"","Stress_244":"0.0001","Stress_245":"0.0001","Stress_246":""},{"Metabolite":"Kynurenic acid","Naive_226":"0.0300","Naive_227":"0.0079","Naive_229":"0.0345","Naive_230":"0.0052","Naive_231":"0.0051","Naive_237":"0.0110","Naive_238":"0.0298","Naive_239":"0.0345","Naive_247":"0.0030","Naive_248":"0.0179","Naive_249":"0.0068","Stress_232":"0.0245","Stress_233":"0.0382","Stress_234":"0.0354","Stress_235":"0.0179","Stress_236":"0.0938","Stress_240":"0.0187","Stress_241":"0.0167","Stress_242":"0.0747","Stress_243":"0.0300","Stress_244":"0.0111","Stress_245":"0.0143","Stress_246":"0.0099"},{"Metabolite":"kynurenine","Naive_226":"0.0824","Naive_227":"0.0646","Naive_229":"0.0939","Naive_230":"0.0898","Naive_231":"0.0612","Naive_237":"0.0759","Naive_238":"0.0738","Naive_239":"0.0942","Naive_247":"0.0607","Naive_248":"0.0793","Naive_249":"0.0622","Stress_232":"0.0645","Stress_233":"0.0778","Stress_234":"0.0898","Stress_235":"0.0984","Stress_236":"0.1841","Stress_240":"0.0849","Stress_241":"0.0935","Stress_242":"0.1181","Stress_243":"0.1102","Stress_244":"0.0722","Stress_245":"0.0742","Stress_246":"0.0840"},{"Metabolite":"Norepinephrine","Naive_226":"","Naive_227":"","Naive_229":"","Naive_230":"","Naive_231":"","Naive_237":"","Naive_238":"","Naive_239":"","Naive_247":"0.3074","Naive_248":"","Naive_249":"","Stress_232":"","Stress_233":"","Stress_234":"","Stress_235":"","Stress_236":"","Stress_240":"","Stress_241":"","Stress_242":"","Stress_243":"","Stress_244":"","Stress_245":"","Stress_246":""},{"Metabolite":"serotonin","Naive_226":"1.5176","Naive_227":"1.7400","Naive_229":"2.1098","Naive_230":"1.6257","Naive_231":"1.5459","Naive_237":"1.6917","Naive_238":"1.7629","Naive_239":"2.1109","Naive_247":"2.3721","Naive_248":"2.4149","Naive_249":"1.9543","Stress_232":"1.3737","Stress_233":"1.0737","Stress_234":"1.0977","Stress_235":"0.8581","Stress_236":"1.7386","Stress_240":"1.0889","Stress_241":"1.2108","Stress_242":"1.7373","Stress_243":"1.2840","Stress_244":"1.3241","Stress_245":"1.4063","Stress_246":"1.4263"},{"Metabolite":"Thyroxine","Naive_226":"0.0027","Naive_227":"0.0035","Naive_229":"0.0022","Naive_230":"0.0028","Naive_231":"0.0020","Naive_237":"0.0034","Naive_238":"0.0022","Naive_239":"0.0006","Naive_247":"0.0044","Naive_248":"0.0025","Naive_249":"0.0024","Stress_232":"0.0051","Stress_233":"0.0026","Stress_234":"0.0075","Stress_235":"0.0017","Stress_236":"0.0064","Stress_240":"0.0022","Stress_241":"0.0071","Stress_242":"0.0040","Stress_243":"0.0005","Stress_244":"0.0023","Stress_245":"0.0043","Stress_246":"0.0052"},{"Metabolite":"Tryptophan","Naive_226":"478.7400","Naive_227":"412.7300","Naive_229":"467.6400","Naive_230":"591.5500","Naive_231":"404.8800","Naive_237":"443.6200","Naive_238":"408.8100","Naive_239":"483.1400","Naive_247":"417.1900","Naive_248":"423.2400","Naive_249":"342.6400","Stress_232":"517.7800","Stress_233":"517.7200","Stress_234":"511.8654","Stress_235":"511.1200","Stress_236":"689.6750","Stress_240":"501.5900","Stress_241":"580.2708","Stress_242":"475.4900","Stress_243":"543.0800","Stress_244":"437.0700","Stress_245":"477.3200","Stress_246":"484.7900"}],

"Metabolites":[{"Metabolite":"Aldosterone","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"10.77","ri_type":"","moverz_quant":"343.1886","":""},{"Metabolite":"corticosterone","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"11.05","ri_type":"","moverz_quant":"329.2097","":""},{"Metabolite":"cortisol","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"10.94","ri_type":"","moverz_quant":"327.1938","":""},{"Metabolite":"Dopamine","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"2.01","ri_type":"","moverz_quant":"137.0592","":""},{"Metabolite":"Kynurenic acid","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"8.1","ri_type":"","moverz_quant":"162.0545","":""},{"Metabolite":"kynurenine","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"4.69","ri_type":"","moverz_quant":"94.0648","":""},{"Metabolite":"Norepinephrine","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"1.56","ri_type":"","moverz_quant":"152.07","":""},{"Metabolite":"serotonin","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"4.08","ri_type":"","moverz_quant":"160.0725","":""},{"Metabolite":"Thyroxine","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"10.93","ri_type":"","moverz_quant":"731.6843","":""},{"Metabolite":"Tryptophan","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"6.43","ri_type":"","moverz_quant":"188.07","":""}]
}

}