{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002705","ANALYSIS_ID":"AN004385","VERSION":"1","CREATED_ON":"May 16, 2023, 2:23 pm"},

"PROJECT":{"PROJECT_TITLE":"Lipidomic Analysis of subventricular zone in conditional deletion of serine racemase in adult mice.","PROJECT_SUMMARY":"Lipid metabolism plays an important role in neurogenesis. The present study was performed to investigate the role of serine racemase in lipid metabolism and adult neurogenesis. Conditional deletion of serine racemase in nestin precursor cells (nestin cre+) showed significant alterations in the different lipid classes in the subventricular zone of adult nestin-cre+ mice based on a lipidomics study. The raw data for the lipidomics study are presented.","INSTITUTE":"Johns Hopkins University School of Medicine","DEPARTMENT":"Neuroscience","LABORATORY":"Solomon H Snyder","LAST_NAME":"Roychaudhuri","FIRST_NAME":"Robin","ADDRESS":"655 West Baltimore Street, Baltimore, Maryland, 21201, USA","EMAIL":"robinroychaudhuri@gmail.com","PHONE":"310-848-4336"},

"STUDY":{"STUDY_TITLE":"Lipidomic Analysis of subventricular zone in adult mice.","STUDY_SUMMARY":"Lipid metabolism plays an important role in neurogenesis. The present study was performed to investigate the role of serine racemase in lipid metabolism and adult neurogenesis. Conditional deletion of serine racemase in nestin precursor cells (nestin cre+) showed significant alterations in the different lipid classes in the subventricular zone of adult nestin-cre+ mice based on a lipidomics study. The raw data for the lipidomics study are presented.","INSTITUTE":"Johns Hopkins University School of Medicine","LAST_NAME":"Roychaudhuri","FIRST_NAME":"Robin","ADDRESS":"655 West Baltimore Street","EMAIL":"robinroychaudhuri@gmail.com","PHONE":"3108484336"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"SR FloxP 1-1",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_SRFlex_1-1_R-A6_1_12172.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"SR FloxP 1-2",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_SRFlex_1-2_R-A6_1_12110.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"SR FloxP 1-3",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_SRFlex_1-3_R-A6_1_12117.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"SR FloxP 2-1",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_SRFlex_2-1_R-A7_1_12173.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"SR FloxP 2-2",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_SRFlex_2-2_R-A7_1_12173.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"SR FloxP 2-3",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_SRFlex_2-3_R-A7_1_12118.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin CreP 1-1",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_NesCze_1-1_R-A8_1_12174.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin CreP 1-2",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_NesCze_1-2_R-A8_1_12112.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin CreP 1-3",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_NesCze_1-3_R-A8_1_12119.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin CreP 2-1",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_NesCze_2-1_R-A9_1_12175.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin CreP 2-2",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_NesCze_2-2_R-A9_1_12113.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin CreP 2-3",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030P_NesCze_2-3_R-A9_1_12120.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"SR Flox 1-1N",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_SRFlex_1-1_R-A6_1_12139.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"SR Flox 1-2N",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_SRFlex_1-2_R-A6_1_12146.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"SR Flox 1-3N",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_SRFlex_1-3_R-A6_1_12153.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"SR Flox 2-1N",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_SRFlex_2-1_R-A7_1_12140.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"SR Flox 2-2N",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_SRFlex_2-2_R-A7_1_12147.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"SR Flox 2-3N",
"Factors":{"Genotype":"Wild type"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_SRFlex_2-2_R-A7_1_12154.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin Cre 1-1N",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_NesCze_1-1_R-A8_1_12141.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin Cre 1-2N",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_NesCze_1-2_R-A8_1_12148.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin Cre 1-3N",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_NesCze_1-3_R-A8_1_12155.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin Cre 2-1N",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_NesCze_2-1_R-A9_1_12142.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin Cre 2-2N",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_NesCze_2-2_R-A9_1_12149.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Nestin Cre 2-3N",
"Factors":{"Genotype":"Nes-Cre+"},
"Additional sample data":{"RAW_FILE_NAME":"2021030N_NesCze_2-3_R-A9_1_12156.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Mouse sub ventricular zone tissue was micro dissected and frozen in liquid nitrogen and stored in -80C till further processing.","COLLECTION_PROTOCOL_FILENAME":"SVZ_Dissection_Protocol-Roychaudhuri_et_al.pdf","SAMPLE_TYPE":"sub ventricular zone tissue"},

"TREATMENT":{"TREATMENT_SUMMARY":"No treatment was performed. Nestin -cre+ mice used in the study are conditional knockout for Serine Racemase.","TREATMENT_PROTOCOL_FILENAME":"Treatment_Protocol-Roychaudhuri_et_al.pdf"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"SVZ Tissues were micro dissected according to protocol.","SAMPLEPREP_PROTOCOL_FILENAME":"SVZ_Dissection_Protocol-Roychaudhuri_et_al.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um)","SOLVENT_A":"Nova MT MixA","SOLVENT_B":"Nova MT MixB","FLOW_GRADIENT":"Nova MT 26 min gradient","FLOW_RATE":"250 microliter/min","COLUMN_TEMPERATURE":"42","METHODS_FILENAME":"MS_Metadata-Roychaudhuri_et_al.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"MS_Metadata-Roychaudhuri_et_al.pdf"},

"MS":{"INSTRUMENT_NAME":"Bruker micrOTOF-Q II QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"m/z 150-1500 range Lipid features were extracted and aligned using NovaMT LipidScreener.","MS_RESULTS_FILE":"ST002705_AN004385_Results.txt UNITS:intensity Has m/z:Yes Has RT:Yes RT units:Minutes"}

}