#METABOLOMICS WORKBENCH wpathmasiri_20140314_9895351_mwtab.txt DATATRACK_ID:58 TEXT OUTPUT STUDY_ID:ST000026 ANALYSIS_ID:AN000046 PROJECT_ID:PR000025 VERSION 1 CREATED_ON 10-02-2015 #PROJECT PR:PROJECT_TITLE Metabolomics Involved in Early Life Antibiotic Exposures PR:PROJECT_TYPE Obesity modeling of antibiotic exposure PR:PROJECT_SUMMARY The project Metabolomics Involved in Early Life Antibiotic Exposures profiled a PR:PROJECT_SUMMARY total of 90 samples from five sub-studies (DuraSTAT, TranSTAT, NOD, EstroSTAT PR:PROJECT_SUMMARY and VG STAT) which included a total of four sample matrices (urine, serum, liver PR:PROJECT_SUMMARY tissue and cecal contents). Within each sub-study, there were three sample PR:PROJECT_SUMMARY matrices except for VG STAT, for which there was only two. For each matrix type PR:PROJECT_SUMMARY within each sub-study 6 samples were analyzed for a total of 18 samples per PR:PROJECT_SUMMARY sub-study (9 of each in VG STAT), the samples were equally divided into PR:PROJECT_SUMMARY STAT/PAT-treated (sub-therapeutic antibiotic treatment (STAT) and therapeutic PR:PROJECT_SUMMARY dose-pulsed antibiotic treatment (PAT)) collected at various time-points versus PR:PROJECT_SUMMARY untreated Controls for each matrix. PR:INSTITUTE New York University PR:DEPARTMENT School of Medicine PR:LABORATORY Blaser Laboratory PR:LAST_NAME Blaser PR:FIRST_NAME Martin J. PR:ADDRESS 550 First Avenue, BCD 690, New York, NY 10016 PR:EMAIL Martin.Blaser@nyumc.org PR:PUBLICATIONS Cho I, Blaser MJ. The human microbiome: at the interface of health and disease. PR:PUBLICATIONS Nature Reviews. Genetics 2012; 13; 260-270 PR:PHONE - #STUDY ST:STUDY_TITLE Metabolomics Involved in Early Life Antibiotic Exposures(DuraSTAT-Cecal) ST:STUDY_TYPE Metabolomics ST:STUDY_SUMMARY In the DuraSTAT sub-study, a total of 18 samples from 8 week old, female C57BL/6 ST:STUDY_SUMMARY mice; comprised of 6 urine samples, 6 cecal content samples and 6 liver tissue ST:STUDY_SUMMARY samples were analyzed. Three mice/matrix were given STAT penicillin and 3 ST:STUDY_SUMMARY mice/matrix were non-treated Controls. The mice were housed with conventional ST:STUDY_SUMMARY bedding and fed a high fat diet. ST:INSTITUTE RTI International ST:DEPARTMENT Systems and Translational Sciences (STS) ST:LABORATORY NIH Eastern Regional Comprehensive Metabolomics Resource Core (RTI RCMRC) ST:LAST_NAME Sumner ST:FIRST_NAME Susan ST:EMAIL ssumner@rti.org ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 6 ST:STUDY_COMMENTS DuraSTAT_Cecal ST:PHONE - ST:ADDRESS - #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN C57BL/6 SU:AGE_OR_AGE_RANGE 8 weeks at collection SU:GENDER Female SU:ANIMAL_ANIMAL_SUPPLIER Jackson Labs SU:ANIMAL_HOUSING Conventional SU:ANIMAL_FEED High Fat Diet SU:SPECIES_GROUP Mammal #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - CDC029 Treatment Group:Control SUBJECT_SAMPLE_FACTORS - CDC030 Treatment Group:Control SUBJECT_SAMPLE_FACTORS - CDC032 Treatment Group:Control SUBJECT_SAMPLE_FACTORS - CPL101 Treatment Group:Pool QC SUBJECT_SAMPLE_FACTORS - CPL102 Treatment Group:Pool QC SUBJECT_SAMPLE_FACTORS - CPL103 Treatment Group:Pool QC SUBJECT_SAMPLE_FACTORS - CPL201 Treatment Group:Pool QC SUBJECT_SAMPLE_FACTORS - CPL202 Treatment Group:Pool QC SUBJECT_SAMPLE_FACTORS - CPL203 Treatment Group:Pool QC SUBJECT_SAMPLE_FACTORS - CDS039 Treatment Group:STAT SUBJECT_SAMPLE_FACTORS - CDS052 Treatment Group:STAT SUBJECT_SAMPLE_FACTORS - CDS054 Treatment Group:STAT #COLLECTION CO:SAMPLE_TYPE cecal contents CO:COLLECTION_TIME 8 weeks CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY Three mice were given STAT penicillin and 3 mice were non-treated controls. STAT TR:TREATMENT_SUMMARY = sub-therapeutic antibiotic treatment. TR:TREATMENT_COMPOUND STAT penicillin #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Frozen cecal contents (cecal) samples were weighed into labeled homogenizer bead SP:SAMPLEPREP_SUMMARY tubes. D20 was added to each tube (500 µL if less than 50 mg and 1000 µL if SP:SAMPLEPREP_SUMMARY more than 100 mg of tissue). The samples were homogenized on a Spex SP:SAMPLEPREP_SUMMARY Geno/Grinder for two 30 second pulses at 1750 rpm. Samples were centrifuged at SP:SAMPLEPREP_SUMMARY 12000 rcf for 5 min. Cecal supernatants were transferred (450/700 µL) into 2.0 SP:SAMPLEPREP_SUMMARY mL 0.2 µm nylon filter tubes and centrifuged at 16000 rcf until all the SP:SAMPLEPREP_SUMMARY homogenate was filtered. The 200 µL remaining aliquot from each 1000 µL cecal SP:SAMPLEPREP_SUMMARY sample was combined in a 10 mL tube and vortexed for 30 seconds to generate SP:SAMPLEPREP_SUMMARY pooled samples for QC during analysis. Three low QC pools were generated by SP:SAMPLEPREP_SUMMARY transferring 450 µL of the pooled homogenate into 2.0 mL 0.2 µm nylon filter SP:SAMPLEPREP_SUMMARY tubes; and three high QC pools were generated by transferring 700 µL of the SP:SAMPLEPREP_SUMMARY pooled homogenate into 2.0 mL 0.2 µm nylon filter tubes and also centrifuged at SP:SAMPLEPREP_SUMMARY 16000 rcf until all the homogenate was filtered. The volume of homogenate needed SP:SAMPLEPREP_SUMMARY to analyze 50 mg/sample and volume of D20 needed to bring the total volume to SP:SAMPLEPREP_SUMMARY 630 µL was calculated. The calculated volumes of filtered supernatant and D20 SP:SAMPLEPREP_SUMMARY were then transferred into BSI-labeled tubes. Chenomx Internal Standard solution SP:SAMPLEPREP_SUMMARY (Chenomx ISTD, Edmonton, Alberta, Canada) contains 5mM SP:SAMPLEPREP_SUMMARY 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS, Chemical Shift Indicator), 100 SP:SAMPLEPREP_SUMMARY mM Imidazole (pH indicator), and 0.2% NaN3 (to inhibit bacterial growth) was SP:SAMPLEPREP_SUMMARY added (70 µl) to the tubes. Samples were vortexed for 30 seconds and SP:SAMPLEPREP_SUMMARY centrifuged at 12000 rcf for 5 minutes. A 600 µL aliquot of the supernatant SP:SAMPLEPREP_SUMMARY was then transferred into 5mm NMR tubes (Wilmad LabGlass, New Jersey, USA) which SP:SAMPLEPREP_SUMMARY were kept on ice until data acquisition. SP:SAMPLEPREP_PROTOCOL_FILENAME DuraSTAT_Cecal_Metabolomics_Procedure.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE - CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:LABORATORY_NAME RTI/ DHHMRI AN:ANALYSIS_TYPE NMR AN:ACQUISITION_DATE 41527 AN:SOFTWARE_VERSION Top Spin 3.2 AN:OPERATOR_NAME Wimal Pathmasiri/ Kevin Knagge/ Jason Winnikie AN:DETECTOR_TYPE NMR AN:DATA_FORMAT NMR #NMR NM:INSTRUMENT_TYPE Bruker NM:NMR_EXPERIMENT_TYPE 1D 1H NM:FIELD_FREQUENCY_LOCK Deuterium NM:STANDARD_CONCENTRATION 0.5mM NM:SPECTROMETER_FREQUENCY 700 MHz NM:NMR_PROBE cyrogenically cooled ATMA inverse probe NM:NMR_SOLVENT D2O NM:NMR_TUBE_SIZE 5mm x 4inch NM:SHIMMING_METHOD topshim NM:PULSE_SEQUENCE noesypr1d NM:WATER_SUPPRESSION presat NM:PULSE_WIDTH 14.84 us NM:POWER_LEVEL 25.704w NM:RECEIVER_GAIN 9 NM:OFFSET_FREQUENCY 3296 Hz NM:CHEMICAL_SHIFT_REF_CPD DSS NM:TEMPERATURE 298.1 K NM:NUMBER_OF_SCANS 64 NM:DUMMY_SCANS 4 NM:ACQUISITION_TIME 2.3243434 NM:RELAXATION_DELAY 2 s NM:SPECTRAL_WIDTH 20.1358 NM:NUM_DATA_POINTS_ACQUIRED 65536 NM:REAL_DATA_POINTS 65536 NM:LINE_BROADENING 0.5 NM:ZERO_FILLING yes NM:APODIZATION lorentzian NM:BASELINE_CORRECTION_METHOD polynomial NM:CHEMICAL_SHIFT_REF_STD DSS NM:INSTRUMENT_NAME Bruker Avance III #NMR_BINNED_DATA NMR_BINNED_DATA:UNITS Peak area NMR_BINNED_DATA_START Bin range(ppm) CDC029 CDC030 CDC032 CPL101 CPL102 CPL103 CPL201 CPL202 CPL203 CDS039 CDS052 CDS054 0.50...0.56 0.00058149 1.6592 0.039301 0 0 0 0.034018 0.0028746 0.0021478 0.013387 0 0 0.56...0.58 0 0.74267 0 0.007206 0 0 0 0 0 0 0 0.0069721 0.58...0.60 0.051165 0.8258 0.089149 0.060972 0.026307 0.045697 0.069541 0 0 0.14516 0.057489 0.042255 0.60...0.65 15.771 10.861 24.633 5.7879 6.6108 6.5931 18.558 13.278 11.576 16.931 12.49 11.39 0.65...0.67 5.4398 3.9547 1.5004 1.0787 1.139 1.1162 0.89154 0.96156 1.1492 6.3879 0.75423 0.42422 0.67...0.73 9.3184 7.7766 3.9113 4.189 4.5693 4.4516 3.8594 3.756 4.3738 11.257 18.352 8.4482 0.73...0.76 0.93826 2.0689 0.045885 0.91323 0.89114 0.88214 0.14137 0.060038 0.22477 2.0752 0.99317 0.90168 0.76...0.78 0.56662 1.4675 0 0.56905 0.58636 0.56519 0 0 0 1.2043 0.50051 0.65229 0.78...0.80 1.1312 1.825 0 0.79163 0.80936 0.8386 0 0 0.01185 1.9406 0.71462 0.93165 0.80...0.82 1.3966 2.1273 0.085969 1.0437 1.1474 1.1011 0.060788 0.16069 0.14051 2.5491 1.231 1.5736 0.82...0.84 2.8253 3.1284 1.1065 1.5676 1.7775 1.7097 1.0449 0.92861 0.84008 3.838 2.4358 3.0993 0.84...0.90 34.974 30.485 14.352 15.274 17.848 16.936 18.255 16.158 16.966 25.502 17.592 22.341 0.90...0.92 11.828 10.11 5.4776 7.1873 7.2929 8.0632 7.5669 6.8666 6.9677 7.8651 10.718 12.564 0.92...0.98 68.285 67.389 33.7 59.048 62.212 65.748 61.028 63.459 61.792 48.212 62.082 67.912 0.98...1.01 13.256 16.9 6.616 17.278 18.067 18.927 15.597 17.689 17.409 8.748 12.027 15.047 1.01...1.07 26.093 22.685 19.112 20.954 22.268 22.808 19.635 22.079 22.08 25.449 29.555 21.061 1.07...1.10 3.8684 3.2427 0 2.0017 1.9742 1.9108 0.43727 1.0207 1.4529 5.0585 4.3153 2.0636 1.10...1.13 4.0913 3.7021 0 2.2169 2.3213 2.2009 1.0233 1.3747 1.5527 5.2247 4.4628 2.4291 1.13...1.15 1.7266 1.8724 0 1.5016 1.5172 1.5229 0.86333 0.8683 0.65598 2.4102 1.8346 1.3856 1.15...1.20 11.516 9.5311 8.0545 45.91 52.272 49.927 49.11 50.533 51.36 20.12 14.265 12.739 1.20...1.22 3.5966 3.3957 0.76001 2.1992 2.4287 2.1514 1.751 1.9746 2.4669 3.9235 4.1818 4.2851 1.22...1.24 5.106 5.1745 1.5714 3.282 4.4194 3.4856 3.3507 3.6433 3.7195 6.3087 4.4038 4.1856 1.24...1.30 38.63 31.022 14.055 14.068 23.11 15.454 24.217 21.908 21.77 51.464 24.682 17.751 1.30...1.36 41.921 35.08 61.018 25.543 29.145 28.006 32.515 31.591 32.007 50.255 32.529 31.002 1.36...1.38 4.91 3.871 1.6441 2.4576 2.5193 2.4629 2.2667 2.3226 2.8483 5.278 6.555 5.2456 1.38...1.40 7.8734 5.9667 2.8057 3.7121 3.7669 3.8316 3.6748 3.5114 4.0865 7.6541 10.344 9.1213 1.40...1.44 12.485 10.121 4.6776 7.2821 7.4859 7.7496 7.335 7.3447 7.549 10.947 13.289 11.193 1.44...1.49 33.114 31.626 26.932 29.908 31.309 32.539 30.229 32.842 31.42 26.618 28.805 32.133 1.49...1.55 19.299 16.865 5.8918 11.104 11.426 11.524 12.12 11.95 12.799 16.509 9.7406 10.194 1.55...1.57 7.226 6.727 2.4179 3.6504 4.182 3.7252 4.3782 4.1718 4.5286 6.8498 2.977 2.8131 1.57...1.59 7.8153 6.4214 3.706 4.0501 4.2412 4.1484 5.4998 4.6995 5.0774 8.3886 7.2898 6.381 1.59...1.61 5.1756 4.189 4.8169 2.9303 2.8309 2.9316 3.1357 2.9027 3.1759 5.0884 5.8688 4.6246 1.61...1.64 7.6716 6.2954 11.969 4.5903 4.5751 4.7568 4.7692 4.5573 4.842 7.1449 7.2534 6.86 1.64...1.67 10.036 9.1539 15.407 7.4504 7.4988 7.8348 7.6394 7.4175 7.7094 8.3316 12.512 12.401 1.67...1.69 7.8821 8.0141 6.7074 6.9342 6.9981 7.4547 6.8921 6.9434 6.8307 6.3305 9.1701 9.9984 1.69...1.74 34.392 32.589 16.676 28.743 29.842 31.179 30.646 31.524 30.547 23.49 34.466 35.552 1.74...1.80 25.726 17.752 24.673 12.779 13.226 13.149 24.268 19.618 19.256 29.034 28 21.622 1.80...1.83 6.1793 5.3448 0 3.7693 3.5237 3.5609 3.1281 3.1981 3.5875 7.0846 7.1785 5.3548 1.83...1.87 8.9373 7.9126 1.0439 5.4535 5.4092 5.5 5.0217 5.0422 5.0921 10.298 8.4719 6.778 1.87...1.93 75.097 56.146 39.933 41.748 45.084 45.067 46.173 47.126 46.772 55.37 35.054 37.933 1.93...1.95 3.407 3.3027 0.25242 2.9725 2.6968 2.7595 2.3536 2.557 3.1877 3.638 3.6908 4.0652 1.95...1.97 3.4621 3.9463 0.19453 3.5928 3.4716 3.5579 3.0754 3.1813 3.5433 3.5983 3.8284 4.3841 1.97...1.99 4.3639 4.9261 1.2128 4.7702 5.002 4.866 4.4033 4.6365 4.7242 4.5641 5.713 6.6736 1.99...2.01 7.3346 7.0046 3.954 6.25 6.9575 6.5193 6.3111 6.5967 6.5956 8.7553 12.451 11.916 2.01...2.06 25.401 25.502 26.971 26.474 28.601 28.327 28.382 27.775 28.844 29.206 37.557 37.116 2.06...2.09 6.1482 5.7792 2.6332 5.8176 5.6186 5.7653 5.684 5.5623 6.1472 6.0223 11.472 10.625 2.09...2.15 33.266 33.848 27.132 29.243 30.53 31.24 30.567 31.095 31.76 26.089 26.985 28.712 2.15...2.18 11.236 9.1409 8.8474 7.8854 7.7891 7.8591 9.11 8.4063 9.3809 11.619 7.2709 6.883 2.18...2.24 12.608 11.204 19.841 9.0872 9.1772 8.5747 9.0389 8.9431 10.039 15.46 15.591 11.918 2.24...2.30 9.3708 10.654 5.979 9.0593 8.2042 8.5318 9.5463 8.6307 9.5588 8.1923 9.5468 10.878 2.30...2.32 2.1694 2.9059 1.557 2.5652 2.1929 2.2784 2.2618 2.1662 2.3089 2.1166 2.5411 3.0158 2.32...2.37 17.207 20.252 24.396 21.991 23.071 23.183 21.927 22.835 23.314 19.681 17.594 20.168 2.37...2.41 3.5633 3.6641 3.9336 3.206 2.3246 2.4477 1.9867 2.0853 2.7811 2.0129 4.7459 4.3488 2.41...2.45 4.0206 6.5149 4.673 6.3812 5.6672 5.853 4.9868 5.5257 5.7091 2.711 4.6059 5.4976 2.45...2.51 1.3527 3.9637 3.1458 4.4698 3.0749 3.0778 2.2937 2.5572 3.2625 1.8936 4.3245 4.5832 2.51...2.57 0.079381 2.807 0 2.894 1.3281 1.3638 0.66927 0.67214 1.0574 0.40721 2.8187 2.7662 2.57...2.60 0 0.99963 0 0.90056 0.24168 0.24372 0.083705 0.089249 0.17301 0.22618 0 0.33876 2.60...2.66 4.2358 7.3585 4.8712 6.8624 5.7838 5.9425 5.1797 5.2768 5.131 3.3989 3.0015 5.3819 2.66...2.69 1.6225 2.9823 3.0985 3.0037 2.5344 2.4341 2.2497 2.2173 2.3579 1.2842 1.4368 2.0293 2.69...2.71 0 0.80983 0 0.64709 0.026386 0.057324 0 0 0 0 0 0.25813 2.71...2.77 0.093755 3.0802 0.18351 2.2921 0.86903 0.64723 0.0031498 0.047089 0.41974 0.63294 0.21578 1.3835 2.77...2.80 1.3086 3.6229 3.9255 3.7208 3.1136 2.9163 2.3511 2.4797 2.5062 1.6872 0.96295 2.0002 2.80...2.84 1.3292 3.2921 2.4148 3.268 2.6419 2.6156 2.0941 2.2436 2.275 0.9138 0.53515 1.287 2.84...2.87 0.19301 2.5063 0 2.0183 0.90796 1.0101 0.63515 0.62223 0.58146 0.4868 0 0.53539 2.87...2.93 17.027 13.503 29.005 9.4655 8.8009 8.8776 21.314 15.523 13.664 20.294 13.474 13.425 2.93...2.96 0.056 1.7993 0 1.3254 0.44341 0.50171 0.20081 0.37501 0.31108 0.04399 0 0.26333 2.96...2.98 0.23374 1.2067 0.26957 0.84574 0.23202 0.25742 0.088058 0.01885 0.0059928 0.31921 0.38814 1.3944 2.98...3.03 17.591 18.522 34.518 18.042 17.847 18.394 17.771 18.624 17.69 11.835 13.771 22.037 3.03...3.07 0.92528 2.8885 0.86843 3.2529 2.1436 2.2486 1.7237 2.0215 2.2253 0.23746 2.2793 3.0875 3.07...3.09 0 0.95153 0 0.83918 0.1756 0.19673 0 0 0 0 0.3215 0.29318 3.09...3.15 5.3468 9.0517 5.0552 8.3277 7.0939 7.302 6.4801 7.2908 6.3761 5.8161 2.6524 4.3223 3.15...3.17 0.63059 1.5954 1.5606 1.6429 1.0202 1.0914 0.81334 0.96497 0.67477 1.0788 0.54758 0.96569 3.17...3.21 11.108 10.695 34.681 11.617 11.169 11.554 10.713 10.942 10.488 13.072 5.8025 11.622 3.21...3.27 23.333 19.058 46.647 19.939 19.68 20.004 20.14 20.753 20.098 19.57 25.373 21.271 3.27...3.30 0.62225 2.6671 1.0492 3.4393 2.517 2.6278 2.2808 2.5529 2.465 0.10641 1.4637 1.7508 3.30...3.36 1.3588 4.7072 4.2487 9.5397 8.3926 8.3097 6.5661 7.3118 6.6356 2.4907 5.5136 5.7583 3.36...3.38 0.3158 1.4105 0.28814 1.4203 0.71333 0.70688 0.63418 0.71692 0.45709 0.51591 0.087787 0.079736 3.38...3.44 17.918 14.765 32.656 15.865 15.492 15.414 14.848 15.934 14.524 16.357 22.453 12.856 3.44...3.46 0.83899 1.9629 1.3327 2.648 2.0462 2.085 2.0009 2.0193 1.8651 1.7552 0.74842 0.57673 3.46...3.48 1.4687 2.6595 1.6163 3.6784 3.0859 3.1468 3.0972 3.0639 2.6975 2.606 1.9086 1.8498 3.48...3.52 5.0466 5.8958 10.861 9.0625 8.6144 8.6889 8.6185 8.7056 7.8685 8.1151 5.252 5.0437 3.52...3.58 18.232 18.803 34.091 23.66 23.516 24.205 22.058 23.126 21.195 23.97 12.004 15.414 3.58...3.62 7.628 7.9622 12.564 11.879 11.516 11.676 11.902 12.149 10.821 9.5194 16.052 12.961 3.62...3.67 16.601 13.649 31.992 44.589 48.091 47.203 47.412 47.828 47.403 27.214 20.042 19.111 3.67...3.69 3.1142 3.5333 5.8866 7.2517 6.9854 7.1108 6.8623 7.0614 7.1778 5.3899 5.3561 4.9192 3.69...3.71 3.6924 4.2204 5.6834 6.4638 6.1913 6.2416 6.5106 6.3724 6.4074 6.0412 5.3624 5.3698 3.71...3.73 4.5882 5.0553 6.4593 8.1077 7.8694 8.0824 8.576 8.4105 8.128 6.0174 7.9431 8.4964 3.73...3.79 25.165 25.352 34.18 37.268 37.665 38.365 38.118 38.44 38.415 23.769 34.449 38.911 3.79...3.84 15.739 16.686 25.046 24.653 24.11 24.661 24.162 24.172 24.657 20.415 21.71 24.06 3.84...3.89 8.9228 9.4916 14.081 14.996 14.227 14.546 14.7 14.477 15.121 10.68 14.046 15.366 3.89...3.94 14.182 15.457 27.007 24.128 23.442 24.093 23.227 23.75 23.96 15.997 19.36 22.432 3.94...4.00 11.928 15.928 15.948 20.557 19.096 19.84 18.198 19.161 19.377 8.2451 14.413 17.526 4.00...4.03 1.4276 2.73 2.3753 4.7541 3.648 3.724 3.6074 3.6412 4.159 1.1119 3.1377 2.8853 4.03...4.07 5.1881 6.113 8.905 7.7601 6.6311 6.5132 6.6409 6.4727 6.9015 5.7072 5.4744 5.0392 4.07...4.12 6.7651 8.7114 15.598 11.72 10.243 10.241 9.5853 9.8354 10.293 8.4981 9.9357 10.278 4.12...4.18 3.3909 5.6394 4.9036 7.7764 5.0741 5.3672 5.2643 4.7663 5.8698 0.39967 9.2976 9.2162 4.18...4.21 0.64859 2.0275 0.58502 2.4915 1.1349 1.2758 1.195 1.0262 1.282 0 2.4381 2.3289 4.21...4.27 2.1932 5.4611 2.8528 6.2693 4.0296 4.0208 3.4869 3.6093 3.6704 1.1961 4.7836 4.3462 4.27...4.31 1.1204 2.9573 2.5375 3.0662 1.1309 1.066 0.96718 0.88773 1.0772 1.7966 2.8193 2.4567 4.31...4.37 0 3.0355 0.57487 3.0949 0.10732 0.19646 0 0.0092536 0 0 3.9353 3.004 4.37...4.42 0.033925 2.7222 0.2821 2.5503 0.40289 0.26731 0.10078 0.046301 0.11465 0.21402 1.9344 0.96401 4.42...4.47 0.00080498 1.9443 0.14014 2.5374 0.73971 0.72027 0.032234 0.24402 0.12192 0 1.3979 0.11923 4.47...4.52 0.40349 1.3169 0.012108 2.0456 1.0778 1.0315 0.50984 0.75683 0.54516 0.64722 0.78103 0 4.52...4.54 0 0.32127 0 0.64991 0.14551 0.14831 0 0 0 0 0 0 4.54...4.60 0.00576 0.76971 0.086453 1.9857 1.3811 1.2857 0.61706 0.84628 0.51205 0.44292 0 0.10909 4.60...4.62 0.0029205 0.10684 0 0.30815 0.14552 0.14711 0 0.046556 0 0 0 0 4.62...4.67 0.69278 0.6276 0.94702 0.74191 0.70136 0.72953 0.24004 0.62839 0.47111 1.0665 0.17937 0.16729 4.67...4.69 0.0050891 0 0 0 0 0 0 0 0.013599 0 0 0 4.69...4.70 0 0 0.018118 0.0020532 0 0.010931 0.0058209 0.00047656 0.0089185 0 0 0 5.10...5.13 0 0 0.061463 0.033264 0.039827 0.034202 0 0.034729 0.061253 0.0017391 0.020034 0.023228 5.13...5.15 0 0 0 0 0 0 0.0090817 0 0 0 0 0 5.15...5.20 0.028335 0.17804 0.13118 0.53092 0.44837 0.54757 0.12701 0.41167 0.23229 0.19847 0 0.16036 5.20...5.24 0.79697 0.91744 1.1141 1.5735 1.6943 1.7064 1.0443 1.3009 1.2262 1.3392 0.45454 0.96953 5.24...5.27 0 0.17452 0.090278 0.36683 0.41409 0.38281 0.020421 0.081862 0 0 0 0.040371 5.27...5.33 0.29728 1.1396 0 0.18839 0.79431 0.14471 0.038545 0.046018 0.036148 0.5256 0 0 5.33...5.36 0 0.019399 0 0 0 0 0 0 0 0 0 0 5.36...5.41 0.0028267 0.030864 0.025911 0.10459 0.12563 0.12873 0.034535 0.17898 0.037859 0.0128 0.54543 0.8647 5.41...5.44 0 0 0.034386 0 0 0 0 0 0 0.00015102 0.093633 0.18318 5.44...5.48 0 0 0 0.0016508 0.0080042 0.0012126 0.041196 0.0059726 0.016159 0.0046414 0 0 5.48...5.53 0.0066469 0 0.0049404 0 0 0.0038281 0 0 0 0 0.010806 0.019983 5.53...5.56 0.01037 0 0.13759 0.021265 0.027373 0.06505 0.0017539 0.017896 0.034606 0.032696 0 0 5.56...5.60 0 0 0 0 0 0 0 0 0 0 0.001442 0 5.60...5.63 0 0 0.065544 0.0019419 0.0049819 0.0047855 0.013958 0.012289 0.0063159 0 0.011952 0.10261 5.63...5.67 0.016774 0 0 0 0 0 0 0 0 0.0057518 0 0 5.67...5.72 0 0 0.0088924 0.0037877 0 0.0093153 0 0 0.032342 0 0 0.00655 5.72...5.77 0 0 0.0075592 0 0.0035232 0 0 0.0071695 0 0.0011019 0 0 5.77...5.82 0.24998 0 1.28 0.88014 0.98264 0.97622 0.60561 0.83163 0.84591 0.69139 0.070122 0.26259 5.82...5.86 0 0 0.01267 0 0 0 0.00095921 0 0.0032355 0 0.0046896 0 5.86...5.92 0.0013475 0 0.019166 0.071611 0.019696 0.072074 0.1472 0.018797 0.013679 0 0.19074 0.0075377 5.92...5.97 0 0 0 0 0 0 0 0 0 0 0 0.0046243 5.97...6.02 0.010381 0 0.039811 0 0 0 0 0.00062356 0 0 0.0091735 0 6.02...6.07 0 0 0 0.0009934 0.0023196 0.0086304 0 0 0 0.0073183 0.007365 0.0023589 6.07...6.11 0.032513 0 0.29467 0 0 0 0.0068716 0.018164 0.012071 0 0.0032574 0.040795 6.11...6.14 0 0 0 0 0.003239 0.0034362 0 0 0.0042669 0 0 0 6.14...6.19 0.00055296 0 0 0.0044503 0.0023648 0 0 0.022717 0 0.018846 0.011023 0.0070124 6.19...6.23 0.01125 0 0 0 0.0014716 0 0.0065565 0 0 0 0 0.0001058 6.23...6.27 0 0 0.0084682 0 0 0 0 0 0 0 0 0 6.27...6.31 0.020058 0 0.0066752 0.0066031 0.012526 0.028329 0 0.0036071 0.018984 0.012344 0.0033315 0.0052996 6.31...6.35 0 0 0 0.003487 0 0 0.016775 0 0 0.0040092 0.0058672 0.0013828 6.35...6.37 0.0050715 0 0.031876 0.055671 0.10792 0.044998 0 0.0019306 0 0 0 0.0038087 6.37...6.42 0 0 0 0.078643 0.10826 0.059501 0 0.025768 0.01058 0.0013716 0 0 6.42...6.47 0 0 0 0 0.0037882 0 0.00042178 0.011718 0 0.0051541 0.0074508 0.00072051 6.47...6.52 0.079219 0 0.12523 0.083408 0.17623 0.12716 0 0.065804 0.017526 0.41845 0.022266 0.068883 6.52...6.55 0.0061144 0 0 0 0 0 0 0 0 0.014035 0 0 6.55...6.58 0 0 0.018641 0 0.0031407 0 0.00093276 0 0.0088193 0 0.0037992 0 6.58...6.62 0 0 0 0.010859 0 0.0076444 0.017532 0.01967 0.012319 0.011995 0 0 6.62...6.65 0 0 0 0 0.0050861 0 0.013299 0 0 0 0.023772 0.0072299 6.65...6.69 0.0048445 0 0.011187 0.00023208 0 0.0055154 0 0 0.0030487 0 0 0 6.69...6.75 0.034636 0.01836 0.01007 0.020124 0.068647 0.026397 0.009004 0.034819 0.0074113 0 0.00059814 0 6.75...6.78 0 0.045795 0 0 0.010222 0 0 0 0 0.0051056 0.00011548 0 6.78...6.82 0.0039422 0.29496 0 0.079647 0.20231 0.048219 0.030415 0.024877 0.0021378 0.11774 0 0 6.82...6.87 0.35185 1.0055 0.55675 0.8382 1.1738 0.92115 0.93743 0.72186 0.75765 0.67448 0.32593 1.0804 6.87...6.93 1.8647 3.2196 1.6474 3.5064 4.0643 4.0898 3.1517 3.3257 3.1938 1.6115 1.7882 3.1765 6.93...6.96 0 0.0659 0 0 0.0093293 0 0 0 0 0 0 0 6.96...7.02 0.076095 0.37197 0.30012 0.22026 0.26773 0.29086 0.19277 0.19921 0.2327 0.64265 0.29344 0.66024 7.02...7.04 0.0063409 0.12997 0 0 0 0 0 0 0 0 0.04933 0.066044 7.04...7.08 0.45935 1.0735 0.36404 1.0605 1.1377 1.2406 1.0864 0.98034 0.86921 0.049205 0.53426 0.56971 7.08...7.10 0 0.22145 0.013383 0 0 0.064545 0.38487 0 0 0 0.264 0.054446 7.10...7.14 1.6743 1.4431 3.4751 0.69715 0.97716 0.9909 1.2895 0.073489 0.11958 1.9687 2.0662 1.8485 7.14...7.15 0.17186 0.20386 0.53325 0.085878 0.13405 0.15304 0.49315 0.27674 0.17821 0.28696 0.26219 0.26909 7.60...7.62 0 0.066586 0.0063867 0 0 0 0 0 0 0 0.011182 0.0093341 7.62...7.68 0.0062525 0.18683 0.047016 0.018202 0.039269 0.0047095 0.048052 0.060669 0.020334 0.034311 0.00017388 0.01491 7.68...7.70 0 0.089964 0 0 0 0.000057905 0 0 0.00007542 0 0 0 7.70...7.75 0.028954 0.48268 0.025012 0.47984 0.5126 0.51217 0.36699 0.40657 0.27465 0.0041016 0.083485 0.30926 7.75...7.79 0.25972 0.89183 0.12625 1.073 1.1751 1.2073 0.0067766 0.0027447 0 0.093326 0.37256 0.54502 7.79...7.84 0 0.23044 0 0.11877 0.12779 0.11262 1.0497 1.0369 0.94307 0 0.0051675 0 7.84...7.88 0.028837 0.24797 0.063544 0.15247 0.1501 0.13455 0.25529 0.049404 0.008737 0 0.21156 0.077253 7.88...7.93 0.22902 0.55694 1.1081 0.89999 0.92885 0.95684 0.69547 0.792 0.5641 0.80617 0.15631 0.32151 7.93...7.96 0 0.054788 0 0 0 0 0.041297 0 0.0021641 0 0 0.0061588 7.96...8.00 0.0058343 0.10826 0.013222 0.0012175 0.011335 0.0011151 0.013054 0.0085146 0 0.016126 0.023145 0 8.00...8.03 0.021986 0.04617 0.015992 0.0082025 0.0014904 0.0098805 0.0012753 0 0.0014487 0.00092114 0.00024778 0.0081762 8.03...8.05 0 0.010038 0.014304 0 0 0 0 0 0 0 0.00081734 0 8.05...8.07 0.0014065 0.021831 0 0.0040619 0 0 0 0.016912 0 0.037365 0 0.011628 8.07...8.09 0.0014349 0.03262 0.015783 0.017321 0.0085565 0.0053673 0 0 0.010742 0 0 0 8.09...8.15 0 0.091314 0.54682 3.518 4.2253 4.0626 0.02111 0 0.0064699 0 8.1449 4.2223 8.15...8.21 7.5751 5.5844 13.474 1.9843 2.1807 2.2567 1.2311 1.7527 1.7178 11.492 0.8654 4.3882 8.21...8.27 0.013812 0.22131 0.59469 0.18172 0.22206 0.19299 0.041081 2.6459 2.2861 0.16761 0.022787 0.14627 8.27...8.32 0 0.096046 0 0.008074 0 0 2.6785 0.040949 0 0 0.021899 0 8.32...8.35 0.057324 0.091814 0.61729 0 0.00094181 0.0015363 0.030438 0.0083261 0 0.023802 0 0.043498 8.35...8.40 0 0 0.012039 0 0 0 0 0 0 0 0 0 8.40...8.43 0 0.023648 0 0.0019916 0 0 0 0 0.0037567 0 0 0.0025266 8.43...8.46 0.059505 0.13365 0.5431 0.070612 0.095754 0.05521 0.019687 0.024984 0.012988 0.043627 0.016507 0 8.46...8.48 0 0 0 0 0.0022284 0 0 0 0 0 0 0.013652 8.48...8.54 0.007524 0 0 0.001579 0.006171 0.0023011 0.019062 0 0.022501 0 0.0046077 0 8.54...8.57 0 0 0 0 0 0 0 0 0 0 0 0 8.57...8.61 0.010296 0.097327 0.17484 0.13185 0.15872 0.11934 0.051932 0.064141 0.029111 0.10735 0.0071209 0.019792 8.61...8.66 0 0 0 0.0042491 0 0 0 0 0 0 0 0 8.66...8.69 0.015993 0.00075526 0 0 0.0043263 0 0.012908 0 0 0 0 0 8.69...8.74 0.0055322 0 0 0.0068754 0.0023781 0.000028422 0 0 0.019123 0 0 0.01226 8.74...8.80 0.0022064 0 0.000030729 0 0 0.0037108 0 0 0 0 0.0039122 0.02124 8.80...8.86 0 0 0.0062959 0.0030119 0 0 0.0030802 0.0017384 0 0.006606 0.009427 0 8.86...8.91 0 0 0 0 0 0 0 0 0 0 0 0 8.91...8.94 0.024904 0.030497 0.10667 0.12029 0.15869 0.06666 0.031124 0.065277 0.037084 0.056284 0.012567 0.032809 8.94...8.96 0 0 0 0.0026378 0.0030208 0 0 0 0 0 0 0 8.96...9.00 0.010405 0 0 0 0.0029197 0.0031982 0.0117 0 0 0 0.01225 0.0041166 NMR_BINNED_DATA_END #END