#METABOLOMICS WORKBENCH jburgess_20140430_9750031_mwtab.txt DATATRACK_ID:95 TEXT OUTPUT STUDY_ID:ST000049 ANALYSIS_ID:AN000085 PROJECT_ID:PR000024
VERSION             	1
CREATED_ON          	10-02-2015
#PROJECT
PR:PROJECT_TITLE                 	Metabolomics Analysis of Thermally Challenged Mayfly Larvae
PR:PROJECT_TYPE                  	MS/NMR
PR:PROJECT_SUMMARY               	The purpose of this study was to examine the metabolic profiles of mayfly
PR:PROJECT_SUMMARY               	(Centroptilum triangulifer) larvae subjected to a thermal challenge. This
PR:PROJECT_SUMMARY               	species is unusual in terms of its ease of culture, and its suitability as a
PR:PROJECT_SUMMARY               	laboratory test organism. Studies complementary to this metabolic study have
PR:PROJECT_SUMMARY               	focused on thermal effects on metabolic rates (respiration) and gene expression
PR:PROJECT_SUMMARY               	patterns in this species and do not support Prtners oxygen limitation paradigm.
PR:PROJECT_SUMMARY               	Our purpose here was to examine how an environmentally realistic thermal
PR:PROJECT_SUMMARY               	challenge affects the physiology of this organism.
PR:INSTITUTE                     	North Carolina State Unversity
PR:DEPARTMENT                    	Environmental and Molecular Toxicology
PR:LABORATORY                    	Buchwalter Laboratory
PR:LAST_NAME                     	Buchwalter
PR:FIRST_NAME                    	David
PR:ADDRESS                       	North Carolina State University Raleigh, NC 27695
PR:EMAIL                         	dbbuchwa@ncsu.edu
PR:PHONE                         	919-513-1129
#STUDY
ST:STUDY_TITLE                   	Metabolomics Analysis of Thermally Challenged Mayfly Larvae (NMR analysis)
ST:STUDY_TYPE                    	Metabolomic analysis of mayflies
ST:STUDY_SUMMARY                 	The purpose of this study was to examine the metabolic profiles of mayfly
ST:STUDY_SUMMARY                 	(Centroptilum triangulifer) larvae subjected to thermal challenge. This species
ST:STUDY_SUMMARY                 	is unusual in terms of its ease of culture, and its suitability as a laboratory
ST:STUDY_SUMMARY                 	test organism. Our purpose here was to examine how an environmentally realistic
ST:STUDY_SUMMARY                 	thermal challenge affects the physiology of this organism. In this study, we
ST:STUDY_SUMMARY                 	obtained several types of insect species and we were able to show that NMR
ST:STUDY_SUMMARY                 	Metabolomics could be used to distinguish among the different types of larvae.
ST:INSTITUTE                     	RTI International
ST:DEPARTMENT                    	Discovery Science Technology
ST:LABORATORY                    	NIH Eastern Regional Comprehensive Metabolomics Resource Core (RTI RCMRC)
ST:LAST_NAME                     	Sumner
ST:FIRST_NAME                    	Susan
ST:ADDRESS                       	3040 E. Cornwallis Road, Research Triangle Park, NC 27709, USA
ST:EMAIL                         	ssumner@rti.org
ST:PHONE                         	919-541-7479
ST:NUM_GROUPS                    	7
#SUBJECT
SU:SUBJECT_TYPE                  	Animal
SU:SUBJECT_SPECIES               	Centroptilum triangulifer;Atherix;Megaloptera
SU:SPECIES_GROUP                 	Insect
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	AD_01	species:Athorix Diptoa | Treatment:Athorix Diptoa control | wing pads:no wing pads	
SUBJECT_SAMPLE_FACTORS           	-	Pooled_QC_01	species:Mayfly, Megalopterans, Athorix Diptoa | Treatment:Pooled QC sample | wing pads:no wing pads	
SUBJECT_SAMPLE_FACTORS           	-	Pooled_QC_02	species:Mayfly, Megalopterans, Athorix Diptoa | Treatment:Pooled QC sample | wing pads:no wing pads	
SUBJECT_SAMPLE_FACTORS           	-	Pooled_QC_03	species:Mayfly, Megalopterans, Athorix Diptoa | Treatment:Pooled QC sample | wing pads:no wing pads	
SUBJECT_SAMPLE_FACTORS           	-	MF_01	species:Mayfly | Treatment:Mayfly control from different study | wing pads:no wing pads	
SUBJECT_SAMPLE_FACTORS           	-	Control_01	species:Mayfly | Treatment:Mayfly control | wing pads:no wing pads	
SUBJECT_SAMPLE_FACTORS           	-	Control_02	species:Mayfly | Treatment:Mayfly control | wing pads:no wing pads	
SUBJECT_SAMPLE_FACTORS           	-	Control_03	species:Mayfly | Treatment:Mayfly control | wing pads:no wing pads	
SUBJECT_SAMPLE_FACTORS           	-	Control_X	species:Mayfly | Treatment:Mayfly controlX | wing pads:wing pads	
SUBJECT_SAMPLE_FACTORS           	-	Heat_Treated_01	species:Mayfly | Treatment:Mayfly Heat Treated | wing pads:no wing pads	
SUBJECT_SAMPLE_FACTORS           	-	Heat_Treated_02	species:Mayfly | Treatment:Mayfly Heat Treated | wing pads:no wing pads	
SUBJECT_SAMPLE_FACTORS           	-	Heat_Treated_03	species:Mayfly | Treatment:Mayfly Heat Treated | wing pads:no wing pads	
SUBJECT_SAMPLE_FACTORS           	-	MG_01	species:Megalopterans | Treatment:Megalopterans control | wing pads:no wing pads	
#COLLECTION
CO:COLLECTION_SUMMARY            	whole insects were collected
CO:COLLECTION_PROTOCOL_COMMENTS  	Original culture material was obtained from the Stroud Water Research Center
CO:COLLECTION_PROTOCOL_COMMENTS  	(Avondale, PA). Larvae were reared at 22°C and fed a periphytic diet. When
CO:COLLECTION_PROTOCOL_COMMENTS  	larvae reached a suitable size, they were separated from their food source for
CO:COLLECTION_PROTOCOL_COMMENTS  	12 hours to evacuate gut contents. Larvae were then divided into two treatment
CO:COLLECTION_PROTOCOL_COMMENTS  	groups – a control group maintained at the culturing temperature of 22°C, and
CO:COLLECTION_PROTOCOL_COMMENTS  	a thermal challenge group.  The thermal challenge group was subjected to a
CO:COLLECTION_PROTOCOL_COMMENTS  	temperature increase at a rate of 1°C per hour. This rate of temperature change
CO:COLLECTION_PROTOCOL_COMMENTS  	is commonly observed in temperate streams. When the treatment temperature
CO:COLLECTION_PROTOCOL_COMMENTS  	reached 30°C, larvae from both control and treatment groups were flash frozen
CO:COLLECTION_PROTOCOL_COMMENTS  	in liquid nitrogen in groups of 12-13 larvae. Each grouping of 12-13 larvae
CO:COLLECTION_PROTOCOL_COMMENTS  	comprised a composite replicate for the metabolic profiling analysis. A total of
CO:COLLECTION_PROTOCOL_COMMENTS  	3 replicates each for the control and thermal treatments were used in this
CO:COLLECTION_PROTOCOL_COMMENTS  	study. Some larvae had developed dark wing pads during the experiment. This is a
CO:COLLECTION_PROTOCOL_COMMENTS  	signal that the larvae were very close to emerging from the aquatic larval phase
CO:COLLECTION_PROTOCOL_COMMENTS  	to a winged sub-adult phase. Enough of these larvae were present in the control
CO:COLLECTION_PROTOCOL_COMMENTS  	treatment to be removed from the control cohort and considered as an independent
CO:COLLECTION_PROTOCOL_COMMENTS  	sample.
CO:SAMPLE_TYPE                   	whole insect
CO:VOLUMEORAMOUNT_COLLECTED      	50 mg
CO:STORAGE_CONDITIONS            	-80C
#TREATMENT
TR:TREATMENT_SUMMARY             	Metabolomics profile of mayfly larvae exposed to heat
TR:TREATMENT_PROTOCOL_COMMENTS   	Heat Treated | Control | ControlX
TR:TREATMENT                     	Abiotic
TR:ANIMAL_ACCLIMATION_DURATION   	exposed to heat (22C to 30C ramp); fasted for 8 hours | control (fasted for 8
TR:ANIMAL_ACCLIMATION_DURATION   	hours) | control (fasted for 6 hours)
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Control and heat-treated mayfly samples were provided in triplicate and were
SP:SAMPLEPREP_SUMMARY            	processed individually. One sample was provided for AD, ControlX, MF control
SP:SAMPLEPREP_SUMMARY            	(from a previous study) and MG larvae. Three replicates of the larvae listed
SP:SAMPLEPREP_SUMMARY            	above having only a single sample were created. Aliquots of 50-300mg of insect
SP:SAMPLEPREP_SUMMARY            	larvae were mixed with degassed 1:1 Acetonitrile:Water solution in a 2 mL snap
SP:SAMPLEPREP_SUMMARY            	cap tube at a concentration of 50 mg/mL.  Megalopterans were substantially
SP:SAMPLEPREP_SUMMARY            	larger than the other larvae and were prepared at 200 mg/mL.  Samples were
SP:SAMPLEPREP_SUMMARY            	homogenized and then centrifuged at 4?C for 5 minutes at 14000rcf.  The
SP:SAMPLEPREP_SUMMARY            	supernatant was removed and placed into a new tube. Samples were centrifuged
SP:SAMPLEPREP_SUMMARY            	again at 4?C for 5 minutes at 14000rcf and a volume of the homogenate
SP:SAMPLEPREP_SUMMARY            	corresponding to 40 mg insect larvae were taken. After homogenization, the three
SP:SAMPLEPREP_SUMMARY            	larval replicates from AD, ControlX, MG and MF control (from a previous study)
SP:SAMPLEPREP_SUMMARY            	were pooled and a single sample created for each larval type. There was
SP:SAMPLEPREP_SUMMARY            	insufficient Mayfly (heat and control) sample material to create an internal
SP:SAMPLEPREP_SUMMARY            	pool, so an external pool was made by combining equal concentration amounts of
SP:SAMPLEPREP_SUMMARY            	AD, MG and MF control (from a previous study) pool quality check (QC) samples.
SP:SAMPLEPREP_SUMMARY            	Samples were completely dried by vacuum centrifuge and were reconstituted by
SP:SAMPLEPREP_SUMMARY            	adding 630 µL of D2O (Aldrich) and 70 µL of Chenomx ISTD solution (Chenomx,
SP:SAMPLEPREP_SUMMARY            	Edmonton, Alberta, Canada).  The samples were vortexed and centrifuged at
SP:SAMPLEPREP_SUMMARY            	14000rcf for 2 minutes. 650 µL of sample was transferred into 5mm NMR tubes and
SP:SAMPLEPREP_SUMMARY            	analyzed by NMR.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	RTI_Metabolomic_Analysis_of_Thermally_Challenged_Larvae_Procedure.docx
SP:PROCESSING_METHOD             	Homogenization and Solvent Removal with SpeedVac
SP:PROCESSING_STORAGE_CONDITIONS 	4°C
SP:EXTRACTION_METHOD             	50% Aqueous  Acetonitrile
SP:EXTRACT_STORAGE               	-80C
SP:SAMPLE_RESUSPENSION           	resuspended in D2O + Chenomx ISTD solution
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	-
CH:INSTRUMENT_NAME               	-
CH:COLUMN_NAME                   	-
#ANALYSIS
AN:LABORATORY_NAME               	DHMRI
AN:ANALYSIS_TYPE                 	NMR
AN:ACQUISITION_DATE              	41624
AN:SOFTWARE_VERSION              	TopSpin 3.0
AN:OPERATOR_NAME                 	Kevin Knagge, Wimal Pathmasiri
AN:DETECTOR_TYPE                 	NMR
AN:DATA_FORMAT                   	Bruker
#NMR
NM:INSTRUMENT_TYPE               	Bruker
NM:NMR_EXPERIMENT_TYPE           	1D 1H
NM:FIELD_FREQUENCY_LOCK          	D20
NM:STANDARD_CONCENTRATION        	0.5 mM
NM:SPECTROMETER_FREQUENCY        	950 MHz
NM:NMR_PROBE                     	5mm Cryogenically Cooled  ATMA
NM:NMR_SOLVENT                   	D20
NM:NMR_TUBE_SIZE                 	5mm
NM:SHIMMING_METHOD               	Topshim (Gradient)
NM:PULSE_SEQUENCE                	noesypr1d
NM:WATER_SUPPRESSION             	yes
NM:PULSE_WIDTH                   	8.68
NM:POWER_LEVEL                   	12.589W
NM:RECEIVER_GAIN                 	4
NM:OFFSET_FREQUENCY              	4468.30 Hz
NM:CHEMICAL_SHIFT_REF_CPD        	DSS
NM:TEMPERATURE                   	298 K
NM:NUMBER_OF_SCANS               	256
NM:DUMMY_SCANS                   	4
NM:ACQUISITION_TIME              	1.73 sec
NM:RELAXATION_DELAY              	2s
NM:SPECTRAL_WIDTH                	18939.395 Hz
NM:NUM_DATA_POINTS_ACQUIRED      	65536
NM:REAL_DATA_POINTS              	65536
NM:LINE_BROADENING               	0.5
NM:ZERO_FILLING                  	Yes
NM:APODIZATION                   	Lorentzian
NM:BASELINE_CORRECTION_METHOD    	Polynomial
NM:CHEMICAL_SHIFT_REF_STD        	DSS
NM:INSTRUMENT_NAME               	Bruker Avance III
#NMR_BINNED_DATA
NMR_BINNED_DATA:UNITS           	Peak area
NMR_BINNED_DATA_START
Bin range(ppm)	AD_01	Pooled_QC_01	Pooled_QC_02	Pooled_QC_03	MF_01	Control_01	Control_02	Control_03	Control_X	Heat_Treated_01	Heat_Treated_02	Heat_Treated_03	MG_01
0.1...0.14	0.1408	0.082533	0.036988	0.042562	0.1306	0.081736	0.092883	0.09953	0.19155	0.13185	0.11115	0.10666	0.011702
0.14...0.17	0.26273	0.14977	0.098442	0.10564	0.21553	0.12838	0.12024	0.12198	0.45039	0.16912	0.1616	0.14368	0.067121
0.17...0.22	0.18891	0.1241	0.059054	0.076289	0.22559	0.15413	0.16573	0.18079	0.34231	0.23373	0.19819	0.20337	0.012249
0.22...0.28	0.30459	0.14419	0.039863	0.097916	0.22083	0.13806	0.16605	0.1658	0.39686	0.22554	0.18463	0.18934	0.023084
0.28...0.3	0.14637	0.052061	0.018759	0.021061	0.066572	0.041312	0.041881	0.039069	0.16209	0.064758	0.054649	0.057583	0.02082
0.3...0.32	0.14185	0.064482	0.022587	0.039686	0.078889	0.048121	0.052397	0.048617	0.15844	0.08024	0.06105	0.065798	0.016926
0.32...0.35	0.19728	0.081689	0.033824	0.048684	0.10229	0.083513	0.081106	0.079358	0.21973	0.11676	0.094009	0.090359	0.031291
0.35...0.37	0.16663	0.076689	0.037946	0.043862	0.090087	0.066456	0.065484	0.072872	0.16469	0.10205	0.081049	0.087877	0.029035
0.37...0.4	0.24285	0.10561	0.061928	0.076105	0.16971	0.1381	0.14895	0.14366	0.23417	0.19562	0.16091	0.1755	0.039555
0.4...0.42	0.19187	0.090748	0.054255	0.065684	0.10618	0.082793	0.09097	0.08934	0.19085	0.13145	0.10549	0.11138	0.038746
0.42...0.44	0.2184	0.11074	0.079597	0.080595	0.146	0.1231	0.13192	0.13731	0.27354	0.17707	0.15072	0.159	0.038181
0.44...0.5	0.91101	0.35077	0.24434	0.28274	0.51544	0.44038	0.44682	0.48249	0.69952	0.63927	0.50511	0.55083	0.17118
0.5...0.52	0.50133	0.14882	0.10441	0.11029	0.18001	0.16721	0.16917	0.17658	0.31535	0.24227	0.19489	0.20508	0.083458
0.52...0.54	0.6203	0.20068	0.15182	0.17788	0.26047	0.23779	0.23161	0.2478	0.4032	0.32415	0.25373	0.28596	0.1087
0.54...0.55	0.18456	0.06354	0.047548	0.060854	0.085807	0.073094	0.081297	0.082224	0.13247	0.1019	0.088255	0.095385	0.037161
0.75...0.77	2.1854	0.64228	0.60277	0.61344	0.51325	0.57938	0.58762	0.58448	1.1741	0.71058	0.63439	0.67181	0.49265
0.77...0.79	3.1221	0.78261	0.75811	0.78635	0.71474	0.83124	0.83362	0.82514	1.4449	0.98317	0.89095	0.93154	0.74235
0.79...0.81	3.9604	1.0242	1.0197	1.0393	0.99305	1.192	1.178	1.1818	1.8171	1.3499	1.2924	1.3206	0.91917
0.81...0.83	5.9024	1.5007	1.5407	1.5336	1.3486	1.7176	1.6847	1.7183	2.6378	1.8861	1.9019	1.8711	1.526
0.83...0.85	9.4391	6.8005	7.0054	6.9298	9.9777	9.9946	10.084	10.413	5.7807	10.401	9.703	9.5986	4.6324
0.85...0.89	26.128	18.451	18.812	19.02	17.789	19.777	18.42	17.294	17.488	19.587	21.566	19.848	17.714
0.89...0.91	12.261	11.594	11.923	12.077	14.278	13.01	13.237	13.498	9.4561	14.142	13.557	13.494	11.644
0.91...0.97	34.015	61.535	61.06	61.212	72.2	60.026	61.454	62.247	27.997	63.536	60.379	64.152	56.832
0.97...1.02	10.309	26.213	25.485	25.308	28.433	23.942	25.4	26.047	12.28	25.491	24.091	26.361	22.275
1.02...1.07	6.4837	14.224	13.792	13.669	17.581	15.481	15.952	16.431	8.6709	16.685	15.643	16.812	11.412
1.07...1.09	2.2783	0.97989	1.0093	0.98777	0.73757	0.98221	0.95223	0.92773	1.1544	0.98764	1.108	1.0502	0.80315
1.09...1.11	2.4157	1.0984	1.1008	1.0882	0.86097	1.0569	1.0438	1.019	1.13	1.0858	1.1903	1.1656	0.82716
1.11...1.13	3.2364	1.3001	1.3367	1.3143	0.99836	1.2303	1.196	1.174	1.272	1.271	1.3671	1.3353	0.90258
1.13...1.18	10.702	7.9387	8.0663	8.0748	8.2518	8.6517	8.6347	8.7795	4.9231	9.1915	9.1258	9.0402	5.8752
1.18...1.21	9.862	6.4999	6.6871	6.6164	6.6393	7.9601	7.9645	8.0356	5.0532	8.2049	8.3294	8.3431	6.0117
1.21...1.23	4.8648	4.0605	4.2795	4.1313	3.9852	5.8659	5.5695	5.6671	3.6558	5.6383	5.8477	5.7717	3.7691
1.23...1.29	34.861	29.763	30.006	28.859	22.441	38.418	33.538	29.774	37.802	32.492	42.578	36.802	15.526
1.29...1.34	31.885	26.723	25.737	26.02	22.334	25.776	24.624	22.825	26.704	24.458	28.502	25.963	26.377
1.34...1.37	6.5413	5.3366	5.5169	5.5191	7.3323	8.16	7.9034	7.542	8.5564	8.1923	8.4891	8.385	4.7087
1.37...1.39	6.4147	4.3372	4.6793	4.7005	3.9853	4.3693	4.2218	3.8601	6.6601	4.2151	4.7039	4.246	4.9095
1.39...1.44	15.5	16.174	16.447	16.469	17.337	15.696	15.844	15.721	11.393	16.459	15.91	16.41	16.87
1.44...1.48	19.304	26.063	25.194	25.095	27.533	22.757	24.193	24.232	20.14	23.193	22.263	24.168	21.71
1.48...1.52	8.3791	12.752	12.86	12.831	14.815	12.948	13.271	13.394	6.6848	13.602	12.992	13.576	10.815
1.52...1.57	11.589	9.2156	9.3199	9.2366	10.812	12.646	12.056	12.078	8.7318	13.027	13.513	13.019	6.8604
1.57...1.6	5.4434	2.676	2.6643	2.7593	2.3134	2.5667	2.4741	2.3107	4.1503	2.4833	2.869	2.5496	2.8736
1.6...1.62	4.6865	3.2973	3.4018	3.4239	4.2973	4.1274	4.1361	4.1105	3.7119	4.2607	4.2283	4.1463	3.4025
1.62...1.66	12.434	12.219	12.308	12.349	12.495	11.26	11.335	11.229	11.578	11.759	11.821	11.812	14.849
1.66...1.68	6.5348	6.5365	6.5429	6.5555	7.2493	5.8368	6.0367	6.0989	4.7054	6.1566	6.0319	6.3554	7.5844
1.68...1.73	16.874	32.076	31.658	31.636	35.424	27.552	28.783	29.674	15.81	30.18	28.307	30.754	32.02
1.73...1.79	20.071	12.977	13.003	13.05	11.772	10.449	10.788	10.556	23.643	10.696	10.806	10.997	15.68
1.79...1.81	3.3527	1.3314	1.3766	1.3932	1.1072	1.1456	1.1095	1.111	1.8716	1.1838	1.2974	1.1852	1.7095
1.81...1.85	7.4901	4.0379	4.1451	4.1895	3.5017	3.4653	3.4006	3.531	3.9564	3.5594	3.881	3.6343	4.9926
1.85...1.87	5.1289	3.6592	3.7031	3.7381	3.3259	3.1101	3.2588	3.2234	3.3835	3.2206	3.1487	3.2909	4.3937
1.87...1.93	24.902	38.582	38.261	38.204	38.69	32.452	33.526	34.535	24.555	34.87	33.173	35.463	40.571
1.93...1.95	5.4422	4.2766	4.3582	4.3807	4.1423	4.1353	4.1356	4.2201	3.3396	4.2221	4.2161	4.2213	4.387
1.95...1.97	5.5595	4.9447	5.0006	4.988	5.2997	5.3352	5.3614	5.46	3.8553	5.2823	5.1443	5.3984	4.8763
1.97...2.03	20.144	18.644	18.73	18.776	18.518	22.723	21.82	20.427	24.587	20.433	21.114	20.61	19.591
2.03...2.09	22.302	22.473	22.627	23.056	19.836	22.765	24.81	23.727	24.859	23.67	22.712	24.083	25.478
2.09...2.14	12.664	21.475	21.145	21.264	24.267	20.347	21.069	22.077	17.907	22.013	20.514	21.788	21.407
2.14...2.17	2.9548	2.6479	2.6614	2.6777	2.9961	3.1749	3.1193	3.2454	2.512	3.3819	3.3017	3.2632	2.2662
2.17...2.19	2.8021	3.0345	3.0318	3.025	3.2067	3.0112	3.0517	3.0961	2.1128	3.0575	3.0412	3.0894	2.7103
2.19...2.25	13.488	11.12	11.246	11.146	10.509	11.643	11.35	10.863	13.338	11.235	12.43	11.638	9.4477
2.25...2.31	15.887	14.733	14.868	15.006	14.401	12.923	13.004	12.846	13.004	13.057	13.327	13.104	14.626
2.31...2.37	17.13	14.34	14.088	14.25	13.347	11.112	11.525	11.386	13.666	11.511	11.23	11.708	13.442
2.37...2.42	5.1656	5.4943	5.5251	5.5345	6.0408	5.8476	5.8894	6.041	5.8281	6.1383	5.9063	6.077	5.6364
2.42...2.48	3.4966	6.734	6.5801	6.5846	7.6145	7.1725	7.3801	8.036	9.8417	7.7472	7.0006	7.4584	7.438
2.48...2.51	1.2662	0.9131	0.92081	0.92124	0.90006	0.90915	0.89855	0.90384	1.7574	0.86295	0.92246	0.86843	0.93961
2.51...2.53	0.92481	0.69621	0.71061	0.70708	0.71287	0.68412	0.69052	0.68965	0.89379	0.66981	0.7075	0.66532	0.67472
2.53...2.56	2.04	2.3424	2.354	2.3631	2.1543	2.0677	2.12	2.0905	3.4525	2.0835	2.0811	1.995	2.4207
2.56...2.6	2.457	1.9983	2.0248	2.0699	1.9063	1.7673	1.7532	1.7763	1.8807	1.8516	1.8532	1.7972	2.1808
2.6...2.62	1.9583	1.1838	1.2043	1.2389	1.1713	1.0492	1.0237	1.0426	1.0362	1.0903	1.1094	1.0723	1.1092
2.62...2.68	7.2295	7.977	7.871	7.8749	8.5961	6.7911	7.1812	7.4318	4.5514	7.2493	6.9424	7.4281	6.9353
2.68...2.7	3.1548	1.8181	1.8608	1.8947	1.5432	1.5898	1.603	1.6273	1.5627	1.5867	1.5588	1.6136	1.7864
2.7...2.72	3.563	1.9824	2.0247	2.0556	1.7207	2.0334	1.9867	2.0101	1.8106	1.9463	1.901	1.9937	1.7445
2.72...2.74	4.6583	2.6193	2.6966	2.6935	2.5371	3.4817	3.2461	3.3131	2.5477	3.1302	3.0544	3.1311	2.1262
2.74...2.8	7.7076	6.7172	6.7088	6.7054	8.0854	12.047	10.443	9.424	7.3429	9.1879	9.7891	9.248	4.957
2.8...2.86	5.778	6.2637	6.0256	6.0664	6.6693	6.7923	6.7884	6.4731	5.2986	6.5705	7.3246	7.1831	5.3096
2.86...2.89	2.7587	1.7637	1.7367	1.7848	2.031	1.8117	1.7852	1.7804	1.576	1.885	1.8563	1.8607	1.8537
2.89...2.89	0.20224	0.10013	0.098123	0.10276	0.12874	0.11962	0.11074	0.10831	0.14815	0.13553	0.13063	0.12271	0.10934
2.93...2.95	2.3795	1.5913	1.5562	1.5489	1.6053	1.4095	1.4917	1.5118	1.6361	1.4674	1.4363	1.5228	1.3059
2.95...2.97	4.3727	1.7254	1.7167	1.7533	1.664	1.5081	1.5677	1.5902	2.7714	1.6119	1.5934	1.6236	1.6892
2.97...3.03	14.62	18.552	18.5	18.563	19.794	15.957	16.562	16.982	10.659	17.291	16.011	17.344	19.161
3.03...3.07	5.9911	6.951	6.9063	6.9317	6.8053	6.3028	6.609	6.5892	5.725	6.4541	6.1426	6.4211	5.6343
3.07...3.09	2.226	1.8922	1.94	1.9618	2.7808	2.9264	2.8842	3.0313	2.3488	3.1694	3.0481	3.0378	1.4163
3.09...3.15	10.959	11.242	11.067	11.124	11.117	9.9989	10.333	10.816	7.7591	10.897	10.254	10.636	12.321
3.15...3.17	3.1473	1.9177	1.9361	1.977	1.8985	1.7171	1.7686	1.7133	3.4834	1.7673	1.7034	1.7121	2.1732
3.17...3.2	11.864	12.024	11.591	11.644	6.658	6.1144	6.3409	6.232	7.555	6.1461	5.7968	6.1616	23.051
3.2...3.26	58.392	45.77	46.255	46.462	30.001	35.189	34.065	34.361	44.119	33.21	32.305	32.831	63.739
3.26...3.31	5.5308	6.3121	6.3345	6.2726	6.2959	7.1015	6.8238	7.1275	6.5107	6.7208	6.6553	6.7972	5.986
3.31...3.35	3.9035	3.5132	3.5024	3.546	2.8136	2.729	2.832	2.6937	5.2315	2.5979	2.6076	2.5415	4.916
3.35...3.37	0.68275	0.60595	0.71997	0.62414	0.71459	0.65219	0.66764	0.68507	0.9477	0.67012	0.68895	0.66065	0.62854
3.37...3.39	2.5531	2.3509	2.4261	2.3279	2.3293	3.7179	3.6745	3.7137	3.8171	3.4132	3.0212	2.929	1.8608
3.39...3.43	6.8098	7.0948	7.1839	7.1305	6.8948	8.8197	9.0213	8.5323	27.559	8.0048	7.4825	7.2081	8.4188
3.43...3.49	7.406	7.16	7.3663	7.1541	7.0775	11.158	11.044	11.182	10.409	10.298	9.1721	8.8398	6.4862
3.49...3.53	5.643	4.5835	4.8976	4.5525	3.0912	4.3462	4.3192	4.3407	5.5853	4.1296	3.8141	3.6657	6.691
3.53...3.56	3.8257	4.3133	4.1518	4.138	4.1037	4.2506	4.3978	4.4992	7.2479	4.0249	3.818	3.9797	3.1703
3.56...3.6	12.014	10.999	10.996	10.955	8.1319	9.1675	9.1937	9.6704	13.957	9.3952	8.8439	9.2821	13.458
3.6...3.64	5.6262	5.2614	5.53	5.3214	6.0851	7.4419	7.4203	7.7861	9.9413	7.4504	7.0419	6.9672	4.6431
3.64...3.68	9.5895	9.712	10.075	9.7113	9.7952	12.764	12.294	12.583	15.773	11.721	11.263	11.553	10.389
3.68...3.71	5.1768	4.8631	5.0012	4.9522	5.1648	7.2964	7.3377	7.2635	9.9492	6.8446	6.0761	6.0137	4.5837
3.71...3.73	4.6156	6.6359	6.7225	6.6371	7.1257	8.0519	8.2459	8.3916	8.5979	7.7243	7.2586	7.337	6.2194
3.73...3.78	19.377	31.394	31.238	31.136	30.59	28.536	29.234	30.116	35.582	29.312	27.464	28.545	31.936
3.78...3.8	3.1585	3.4543	3.5176	3.461	3.5509	3.3629	3.6715	3.6688	4.3985	3.2636	3.1293	3.4996	3.9296
3.8...3.86	16.827	19.505	19.81	19.491	18.354	19.948	20.079	20.253	27.321	19.44	17.781	17.851	21.476
3.86...3.92	18.271	16.565	16.908	16.788	16.204	19.975	19.524	19.942	17.938	18.403	17.171	17.389	16.569
3.92...3.96	9.693	12.669	12.732	12.706	13.594	14.133	14.236	14.563	13.909	14.047	13.336	13.682	12.196
3.96...4.01	16.503	16.791	16.978	16.772	15.166	13.753	13.894	14.436	12.621	14.179	13.439	13.841	15.642
4.01...4.07	10.691	9.64	9.6854	9.8167	10.871	10.638	10.432	10.514	13.526	10.873	10.661	10.478	11.828
4.07...4.12	14.75	11.163	11.165	11.358	9.2055	9.5527	9.0093	9.1227	10.19	9.3244	9.7077	9.0813	12.738
4.12...4.17	13.546	10.697	10.965	11.191	8.5124	7.4877	7.4643	7.6694	6.692	8.1328	8.0094	7.8231	14.251
4.17...4.21	5.2393	5.0598	5.195	5.3099	5.9197	5.2098	5.3127	5.531	4.5633	5.8202	5.6426	5.5472	4.7369
4.21...4.26	7.9957	7.7538	7.759	7.8632	8.0977	7.2849	7.3794	7.6386	8.1532	7.7802	7.7044	7.6883	7.5818
4.26...4.29	6.2266	4.435	4.5144	4.5528	3.6179	3.2503	3.211	3.2175	6.074	3.4973	4.04	3.6048	4.3524
4.29...4.34	9.1816	5.9217	6.0772	6.1813	6.0213	6.2253	5.9778	6.2213	6.7076	6.0688	6.298	6.0791	6.8857
4.34...4.38	6.7244	5.6167	5.7769	5.8998	5.9839	5.4181	5.4861	5.4388	8.1731	5.6251	5.6411	5.2893	7.0054
4.38...4.44	6.6569	6.5495	6.6709	6.7474	6.3161	5.025	5.1314	5.1204	5.1548	5.3121	5.4389	5.1551	7.155
4.44...4.49	3.7749	2.928	3.0341	3.0742	3.2086	2.6859	2.7112	2.7526	3.3801	2.8334	2.9176	2.7352	3.0032
4.49...4.52	2.2984	1.2975	1.4517	1.3948	2.153	1.9931	1.9946	2.0182	4.1483	2.0376	2.1068	1.9717	1.2992
4.52...4.55	1.29	0.69087	0.85804	0.73582	0.80514	0.72183	0.72653	0.73703	1.24	0.71584	0.77845	0.706	0.72773
4.55...4.61	4.4838	2.0985	2.2003	2.2486	2.1356	2.0259	1.9812	1.9682	3.789	1.9746	2.1463	1.9135	2.0339
4.61...4.66	4.724	3.4835	3.658	3.6015	3.4522	4.7175	4.6329	4.6349	5.0904	4.2366	4.0103	3.714	2.8076
4.66...4.7	0.54175	0.43858	0.45525	0.46732	0.4459	0.40578	0.38965	0.42014	0.37018	0.3708	0.49332	0.37221	0.36198
4.8...4.82	0	0	0	0	0	0	0	0	0	0	0.0031726	0	0
4.82...4.84	0.007054	0.0058906	0	0	0.011299	0.006204	0	0.013154	0	0	0.035996	0	0
4.84...4.87	0.11293	0.076241	0.061213	0.070638	0.11021	0.09785	0.099098	0.11232	0.060593	0.053737	0.13663	0.078408	0.013089
4.87...4.9	0.11516	0.10297	0.1056	0.10561	0.13169	0.23909	0.22819	0.24901	0.57713	0.22032	0.276	0.23174	0.038372
4.9...4.92	0.14105	0.1795	0.16087	0.16049	0.16861	0.18973	0.17725	0.18882	0.20082	0.1475	0.2332	0.16547	0.20031
4.92...4.97	0.23924	0.28182	0.26898	0.27047	0.40055	0.63641	0.61495	0.65461	0.38094	0.52521	0.60354	0.47317	0.16726
4.97...5	0.11837	0.088524	0.083051	0.079236	0.15512	0.24349	0.21907	0.2337	0.12992	0.19523	0.26084	0.18622	0.036788
5...5.02	0.081356	0.088242	0.076843	0.070435	0.097552	0.13941	0.12187	0.10711	0.065166	0.095008	0.16501	0.097928	0.060183
5.02...5.08	0.34338	0.28788	0.27805	0.27006	0.34763	0.50105	0.46291	0.46983	0.23289	0.36163	0.51343	0.41283	0.16469
5.08...5.1	0.12024	0.10232	0.098006	0.083944	0.10909	0.17723	0.15927	0.15367	0.11811	0.14219	0.18762	0.14493	0.03472
5.1...5.12	0.10806	0.099858	0.097921	0.08577	0.096769	0.16464	0.15602	0.16694	0.073715	0.13619	0.20025	0.14973	0.041604
5.12...5.14	0.17366	0.1088	0.11464	0.11447	0.11583	0.19091	0.1747	0.17968	0.068576	0.14015	0.21162	0.17176	0.051074
5.14...5.16	0.26899	0.1309	0.13879	0.13328	0.089046	0.17976	0.16668	0.16701	0.10875	0.13407	0.19665	0.15521	0.087644
5.16...5.19	0.77028	0.44345	0.44574	0.43224	0.17345	0.37904	0.33994	0.3397	0.5395	0.33603	0.40799	0.32722	0.6655
5.19...5.23	2.9322	1.6434	1.6929	1.6331	1.235	2.5857	2.4275	2.3369	3.371	2.1416	2.2875	1.9516	0.82759
5.23...5.25	0.53534	0.37645	0.3984	0.36778	0.35426	0.86033	0.75821	0.74451	0.2892	0.63516	0.70359	0.66435	0.32684
5.25...5.27	0.82656	0.70115	0.7317	0.7055	0.91196	2.2433	1.8968	1.7672	0.80821	1.5797	1.799	1.6776	0.15168
5.27...5.33	3.7761	3.4429	3.3245	3.2745	4.1062	10.119	7.9682	6.4368	7.5072	6.4885	8.8321	7.1227	0.52829
5.33...5.37	0.84306	0.85945	0.80132	0.8091	0.90352	2.1366	1.7942	1.4033	1.8224	1.4007	2.0248	1.5867	0.23676
5.37...5.42	1.7421	0.62625	0.65783	0.62625	0.89287	1.778	1.6788	1.5075	6.1383	1.3201	1.4298	1.2783	0.33811
5.42...5.48	0.29897	0.6238	0.60582	0.61158	0.75036	0.98772	0.97622	0.98079	0.28098	0.90813	0.99931	0.93825	0.87684
5.48...5.52	0.109	0.33453	0.31879	0.30493	0.48889	0.69517	0.66505	0.73571	0.3006	0.72723	0.76179	0.7055	0.31661
5.52...5.58	0	0.077245	0.06627	0.058561	0.12972	0.17204	0.16726	0.19916	0.12043	0.1013	0.2238	0.13744	0
5.58...5.63	0.20813	0.13931	0.11305	0.12081	0.14034	0.13019	0.13928	0.15975	0.28929	0.052768	0.17608	0.090652	0.068579
5.63...5.67	0	0.043567	0.018975	0.039135	0.09953	0.066274	0.054186	0.078549	0.040496	0.0048699	0.11283	0.052529	0
5.67...5.71	0.029985	0.053349	0.039339	0.030386	0.079601	0.047366	0.032686	0.07574	0	0	0.10701	0.0013024	0.034524
5.71...5.76	0.05021	0.055121	0.013429	0.03541	0.084423	0.048586	0.041973	0.058497	0.026045	0	0.11113	0.019366	0
5.76...5.81	0.088066	0.098775	0.088988	0.086555	0.18684	0.073448	0.078393	0.085173	0.16439	0.011556	0.1265	0.043536	0.016345
5.81...5.84	0.067067	0.047382	0.050826	0.050025	0.093879	0.022037	0.021308	0.035294	0.031642	0	0.049495	0.0084641	0.042951
5.84...5.87	0.10816	0.12732	0.11456	0.12055	0.1817	0.055561	0.04685	0.082295	0.039852	0.019582	0.10575	0.066246	0.13229
5.87...5.91	0.25474	0.48695	0.41791	0.42817	0.34485	0.16964	0.19702	0.21371	0.35159	0.15837	0.24838	0.19421	0.56949
5.91...5.94	0.14827	0.15125	0.13084	0.14898	0.26589	0.09456	0.10714	0.1309	0.19323	0.085988	0.15831	0.10568	0.081467
5.94...6	0.31632	0.32844	0.30761	0.32574	0.48071	0.2354	0.26664	0.29442	0.45345	0.22116	0.37323	0.25732	0.067094
6...6.02	0.043577	0.063295	0.045033	0.057478	0.063027	0.017747	0.014193	0.019375	0.0283	0	0.02794	0.0027684	0.05373
6.02...6.05	0.081127	0.1289	0.11925	0.11421	0.17206	0.054348	0.059437	0.088141	0.16796	0.041469	0.11732	0.0614	0.1209
6.05...6.11	0.35084	0.82024	0.76733	0.78332	0.47122	0.28306	0.33854	0.37089	0.63051	0.32467	0.4277	0.38863	0.68553
6.11...6.14	0.54105	0.081179	0.098096	0.12228	0.68049	0.63635	0.63906	0.635	2.3943	0.62187	0.70914	0.60534	0
6.14...6.19	0.2162	0.089153	0.083426	0.089274	0.076192	0.011997	0.023638	0.039486	0.094442	0	0.065212	0	0.061785
6.19...6.22	0.048918	0.039349	0.020338	0.020887	0.054765	0.0079778	0.018213	0.02887	0.062399	0	0.037324	0.00022351	0
6.22...6.26	0.11817	0.072735	0.030929	0.037222	0.091444	0.023824	0.024815	0.042064	0.095292	0	0.063131	0.0089361	0.0124
6.26...6.29	0.086476	0.062704	0.044091	0.036246	0.073813	0.021968	0.023764	0.034482	0.073326	0	0.050151	0.0098717	0.0024932
6.29...6.35	0.21073	0.13207	0.083108	0.10578	0.1479	0.068652	0.090111	0.10117	0.21435	0.025349	0.12738	0.047302	0.0036825
6.35...6.37	0.092264	0.051024	0.034837	0.035974	0.059216	0.023605	0.034392	0.030961	0.059563	0	0.033167	0.018085	0.0031671
6.37...6.41	0.17735	0.099448	0.075054	0.074167	0.11581	0.071824	0.07563	0.08835	0.16256	0.034238	0.098166	0.043635	0.039098
6.41...6.45	0.17142	0.10707	0.076616	0.090426	0.1075	0.07099	0.069823	0.075753	0.21664	0.036279	0.094601	0.045891	0.049548
6.45...6.47	0.10415	0.059888	0.044894	0.049113	0.065158	0.044257	0.049495	0.059718	0.10849	0.024963	0.05327	0.041626	0.025936
6.47...6.51	0.33741	0.22374	0.18684	0.18433	0.18626	0.1144	0.11243	0.13936	0.29956	0.10902	0.17432	0.11538	0.13837
6.51...6.53	0.1314	0.079352	0.073109	0.086421	0.086462	0.061444	0.066339	0.073969	0.18102	0.050544	0.082734	0.051951	0.061156
6.53...6.55	0.11654	0.086615	0.056973	0.064265	0.097351	0.067653	0.072788	0.074738	0.15088	0.057313	0.087821	0.063891	0.062671
6.55...6.59	0.25477	0.1693	0.1264	0.15888	0.18	0.14759	0.1484	0.1589	0.37437	0.12689	0.16881	0.13555	0.079927
6.59...6.63	0.40898	0.23829	0.20559	0.20826	0.243	0.20751	0.22087	0.23046	0.54714	0.20168	0.26445	0.21504	0.13785
6.63...6.65	0.27251	0.10527	0.10151	0.10813	0.11346	0.11285	0.11621	0.11868	0.26188	0.10289	0.11497	0.10695	0.067347
6.65...6.67	0.43243	0.11627	0.10095	0.10323	0.1153	0.12969	0.12406	0.13909	0.26426	0.10786	0.12068	0.11635	0.085513
6.67...6.71	0.77148	0.34179	0.33735	0.36104	0.48842	0.65289	0.65519	0.69887	1.4821	0.57849	0.54649	0.57154	0.23905
6.71...6.74	0.61842	0.22193	0.20725	0.21536	0.18656	0.36757	0.31066	0.36336	0.40007	0.27179	0.26081	0.26625	0.41687
6.74...6.76	0.8206	0.18597	0.17442	0.1717	0.18939	0.19226	0.18821	0.20242	0.45154	0.18155	0.18586	0.18899	0.16494
6.76...6.78	0.77219	0.1893	0.16917	0.17744	0.18119	0.1766	0.17668	0.18607	0.47483	0.18313	0.19087	0.17616	0.17562
6.78...6.8	1.0844	0.33145	0.32241	0.32623	0.37055	0.41538	0.42855	0.44993	0.88758	0.39605	0.39747	0.39549	0.3767
6.8...6.86	1.8737	1.6519	1.694	1.7722	3.1642	2.3585	2.2836	2.1749	2.3972	2.3843	2.3866	2.1749	1.9165
6.86...6.9	1.2989	6.0115	5.8006	5.9789	6.4324	5.8466	6.2178	6.2181	3.8506	6.213	5.8087	6.0177	4.8335
6.9...6.93	1.3384	0.35638	0.35681	0.34937	0.27439	0.23204	0.24444	0.25105	0.6832	0.2154	0.27497	0.24933	0.2997
6.93...6.95	0.84173	0.27439	0.26333	0.25678	0.22494	0.17684	0.18732	0.19646	0.53116	0.17824	0.19951	0.19312	0.22637
6.95...6.98	1.0228	0.39747	0.37994	0.39136	0.3316	0.28113	0.31229	0.3048	0.68677	0.28781	0.29999	0.29554	0.37247
6.98...7	1.0942	0.47114	0.44413	0.4464	0.31963	0.25914	0.27382	0.27045	0.68664	0.26225	0.28136	0.27216	0.51698
7...7.02	1.3725	0.53784	0.52626	0.53195	0.49992	0.41102	0.46543	0.45493	1.2069	0.41322	0.41877	0.43021	0.53858
7.02...7.04	0.90522	0.66367	0.66785	0.63549	0.52765	0.42924	0.51539	0.46677	1.0473	0.43423	0.41982	0.44769	0.61332
7.04...7.08	3.127	2.9191	2.8318	2.8446	2.481	1.8998	1.9568	2.0748	2.506	1.9859	1.9957	2.0848	2.751
7.08...7.12	3.0194	1.4844	1.4787	1.5074	2.3401	1.6847	1.6444	1.5707	2.4318	1.7631	1.7782	1.6142	1.428
7.12...7.16	2.347	2.1035	2.09	2.1544	3.1098	1.9964	2.046	1.9083	2.1226	2.2799	2.3015	2.1625	2.1217
7.16...7.2	3.9987	9.53	9.2916	9.3479	9.4433	7.9548	8.911	8.7691	7.4746	8.4082	7.7908	8.6455	8.2223
7.2...7.22	1.6124	1.0789	1.07	1.0421	0.76831	0.90256	0.61828	0.56888	1.3915	0.94329	0.92228	0.67917	1.2163
7.22...7.24	2.3161	1.9077	1.9176	1.8494	1.4886	0.95714	1.1921	1.083	2.3121	1.0293	1.1082	1.1833	2.0442
7.24...7.25	1.0722	0.95068	0.9703	0.90237	0.67922	0.46585	0.53265	0.48106	1.1366	0.48928	0.48754	0.51915	0.98967
7.35...7.39	0.36398	3.249	3.3143	3.3525	3.1993	2.3283	2.4408	2.5499	1.4185	2.5589	3.2163	2.4988	2.9769
7.39...7.45	1.0884	3.6968	3.5962	3.6777	4.5977	3.7207	3.9382	4.2503	1.1457	3.8771	4.0128	4.0774	4.0163
7.45...7.48	0	0	0	0	0	0	0	0	0	0	0	0	0
7.48...7.54	0	0.24598	0.28618	0.28724	0.50655	0.31169	0.3587	0.4282	0.15835	0.20513	0.44336	0.30553	0.15427
7.54...7.57	0	0	0	0	0	0	0	0	0	0	0	0	0
7.57...7.59	0	0	0	0	0	0	0	0	0	0	0	0	0
7.59...7.64	0.039014	0.010127	0.028911	0.038688	0.12768	0.017603	0.036846	0.084438	0.20288	0	0.096395	0	0
7.64...7.67	0	0.0055365	0.0064777	0.026872	0.054257	0.024722	0.022226	0.033229	0.049726	0	0.077446	0	0
7.67...7.69	0.0079271	0	0	0	0.043263	0	0.0064199	0.020037	0.002166	0	0.027045	0	0
7.69...7.75	0.08209	0.40412	0.39899	0.38537	0.54436	0.368	0.43151	0.46762	0.5633	0.3145	0.44447	0.40249	0.31133
7.75...7.78	0.02274	0.036293	0.031855	0.031312	0.048518	0.0098676	0.017166	0.024654	0.18308	0	0.015741	0	0
7.78...7.84	1.3256	1.7535	1.6965	1.693	1.5537	1.2006	1.2247	1.4294	0.99049	1.2009	1.3569	1.2573	1.3264
7.84...7.88	0.33692	0.38557	0.37762	0.39411	0.20946	0.13234	0.16024	0.18478	0.37767	0.048786	0.1689	0.085454	0.28937
7.88...7.94	0.23875	0.3109	0.31988	0.30231	0.33733	0.13156	0.1465	0.17893	0.10718	0.049129	0.20583	0.10928	0.17697
7.94...7.97	0.19625	0.20713	0.19286	0.19488	0.26656	0.15712	0.18615	0.20573	0.35767	0.13812	0.21682	0.15451	0.028695
7.97...8.03	0.2643	0.42293	0.38164	0.39755	0.52893	0.16217	0.21768	0.26065	0.12111	0.12876	0.26233	0.22606	0.33102
8.03...8.05	0.096597	0.10853	0.10516	0.10835	0.090253	0.078641	0.042022	0.054321	0.053575	0.0091734	0.059103	0.0083199	0
8.05...8.08	0.17648	0.10721	0.10588	0.10457	0.18428	0.037155	0.094814	0.090423	0.11357	0.063765	0.12716	0.01186	0.012986
8.08...8.13	0.5824	0.26578	0.2463	0.27374	0.46815	0.2319	0.25256	0.30566	0.29321	0.10816	0.21262	0.2533	0.15913
8.13...8.15	0.33176	0.065217	0.07956	0.073062	0.085473	0.091459	0.04818	0.06587	0.13431	0.098757	0.11841	0.057702	0.017835
8.15...8.17	0.23265	0.14518	0.15057	0.13822	0.10859	0.099958	0.066834	0.070455	0.45567	0.12871	0.17637	0.046727	0.070307
8.17...8.19	0.25535	0.55467	0.78643	0.47055	0.22035	0.092671	0.16761	0.15439	8.0344	0.098394	0.12127	0.10008	0.45416
8.19...8.21	0.63221	5.8131	5.613	5.8255	0.27619	0.10521	0.33842	0.21398	0.33237	0.06874	0.13018	0.12587	0.65846
8.21...8.26	8.7031	0.81029	0.71337	0.90106	4.9172	0.92885	4.5898	4.4121	1.7749	0.75629	0.90144	4.7332	8.0669
8.26...8.32	0.2616	0.095016	0.076787	0.098569	0.10154	3.7966	0.0054553	0.054254	0.077116	3.9122	4.0026	0.054064	0
8.32...8.37	0.31713	0.81359	0.75854	0.74342	0.34346	0.16685	0.23169	0.28894	0.17896	0.16972	0.36303	0.24269	0.74446
8.37...8.4	0.042385	0	0	0	0.013458	0.015314	0	0	0	0	0.020598	0	0
8.4...8.43	0.062456	0	0	0	0.050348	0.0075462	0.013412	0.018119	0.19026	0	0.064823	0	0
8.43...8.48	0.12021	0.01912	0.0037613	0.013693	0.071351	0.028587	0.029995	0.047106	0.19574	0	0.064772	0	0
8.48...8.51	0.012953	0	0	0	0.036816	0.027775	0.02409	0.051744	0.10184	0	0.03493	0	0
8.51...8.56	0.40847	0	0	0	0.079698	0.10142	0.06566	0.12538	0.42076	0	0.088966	0	0
8.56...8.61	0.049163	0	0	0	0.67577	0.67042	0.68175	0.68815	2.2134	0.60928	0.68835	0.60843	0
8.61...8.64	0.028614	0	0	0	0	0	0	0	0.0007583	0	0	0	0
8.64...8.66	0.041561	0	0	0	0.0058695	0	0	0.0061524	0	0	0	0	0
8.66...8.71	0.0551	0	0	0	0.025201	0	0.007132	0.023265	0.025272	0	0.038681	0	0
8.71...8.75	0.0059745	0	0	0	0.025079	0	0	0.019867	0	0	0.026346	0	0
8.75...8.78	0	0	0	0	0.012689	0	0	0.0089406	0.023866	0	0.015761	0	0
8.78...8.83	0.0069991	0	0	0	0.06887	0.024505	0.027978	0.046649	0.14163	0	0.063297	0	0
8.83...8.86	0.0024657	0	0	0	0.016132	0	0.0017094	0.012262	0.024709	0	0.014594	0	0
8.86...8.88	0	0	0	0	0.015683	0.0041386	0.00020172	0.014401	0	0	0.014218	0	0
8.88...8.93	0.046026	0.018314	0	0	0.070526	0.028988	0.0286	0.070963	0.063662	0	0.075011	0	0
8.93...8.97	0	0	0	0	0.028836	0	0.0025021	0.026723	0	0	0.023707	0	0
8.97...9	0.02904	0.01218	0	0	0.0074127	0	0	0.0081342	0	0	0.019922	0	0
9...9.05	0.023015	0	0	0	0.028624	0	0	0.014634	0.025023	0	0.016053	0	0
9.05...9.1	0.0058254	0.0018238	0	0	0.043215	0.00011715	0.006284	0.023469	0.0077599	0	0.025695	0	0
9.1...9.12	0	0	0	0	0.0096545	0	0	0.013198	0.0033375	0	0.01777	0	0
9.12...9.16	0	0	0	0	0.044182	0.019702	0.012059	0.037641	0.18589	0	0.059005	0	0
9.16...9.2	0	0	0	0	0.054683	0.013238	0.032069	0.041058	0.039708	0	0.057927	0	0
9.2...9.23	0	0	0	0	0.013078	0	0	0.012667	0	0	0.001954	0	0
9.23...9.25	0	0	0	0	0.013098	0	0	0.007143	0	0	0.0040605	0	0
9.25...9.27	0.0038739	0	0	0	0.0084793	0	0.0066458	0.0060331	0	0	0.012097	0	0
9.27...9.3	0.007227	0	0	0	0.018492	0	0	0.010734	0.020974	0	0.01838	0	0
9.3...9.35	0.045879	0.0082594	0	0	0.035425	0.019786	0.014921	0.028085	0.18211	0	0.053706	0	0
9.35...9.4	0	0	0	0	0.021623	0	0	0.0076558	0.026921	0	0.0049312	0	0
9.4...9.45	0	0	0	0	0.018316	0	0.00076066	0.00010346	0	0	0.0075347	0	0
9.45...9.5	0	0	0	0	0.031083	0	0	0.0071238	0	0	0.0057209	0	0
9.5...9.53	0	0	0	0	0.014316	0	0	0	0.0084739	0	0	0	0
9.53...9.56	0	0	0	0	0.0053203	0	0	0	0.012649	0	0	0	0
9.56...9.6	0.018365	0.022969	0.019546	0.012356	0.033255	0	0	0.0015704	0.0066594	0	0.0031663	0	0
NMR_BINNED_DATA_END
#END