#METABOLOMICS WORKBENCH araskind_20150313_9067591_mwtab.txt DATATRACK_ID:221 STUDY_ID:ST000147 ANALYSIS_ID:AN000234 PROJECT_ID:PR000129 VERSION 1 CREATED_ON 01-15-2019 #PROJECT PR:PROJECT_TITLE Metabolic effects of Noxa PR:PROJECT_SUMMARY labeled glutamine metabolomics on Noxa expressing cells PR:INSTITUTE University of Minnesota PR:DEPARTMENT Masonic Cancer Center PR:LABORATORY Kelekar Lab PR:LAST_NAME Kelekar PR:FIRST_NAME Ameeta PR:ADDRESS 420 Delaware Street SE,Minneapolis, MN 55455 PR:EMAIL ameeta@umn.edu PR:PHONE 612-626-2358 PR:DOI http://dx.doi.org/10.21228/M85C7W #STUDY ST:STUDY_TITLE 13C targeted metabolomics ST:STUDY_SUMMARY Jurkat parental cells (JP6) or cells stably expressing the pCDNA Noxa vector ST:STUDY_SUMMARY (N5) cells were cultured to confluence and counted. 20E6 cells in triplicate ST:STUDY_SUMMARY were resuspended in glucose free or glutamine free medium for 2 hours. Cells ST:STUDY_SUMMARY were then pelleted and resuspended in 13C-1,2 Glucose (10mM) or 13C-U-glutamine ST:STUDY_SUMMARY (4mM) for 24 hours. Cells were pelleted and spent medium was collected. Cell ST:STUDY_SUMMARY pellets were washed 1X with ice cold PBS and cell pellets were resuspended in ST:STUDY_SUMMARY 400ul -20 methanol. Cells and media were snap frozen in liquid nitrogen and ST:STUDY_SUMMARY stored at -80. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@umich.edu ST:PHONE - #SUBJECT SU:SUBJECT_TYPE Human cells SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:SPECIES_GROUP Human #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0004256 S00015033 Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004256 S00015034 Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004256 S00015035 Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004256 S00015045 Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium SUBJECT_SAMPLE_FACTORS SU0004256 S00015046 Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium SUBJECT_SAMPLE_FACTORS SU0004256 S00015047 Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium SUBJECT_SAMPLE_FACTORS SU0004257 S00015036 Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004257 S00015037 Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004257 S00015038 Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004257 S00015048 Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium SUBJECT_SAMPLE_FACTORS SU0004257 S00015049 Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium SUBJECT_SAMPLE_FACTORS SU0004257 S00015050 Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium SUBJECT_SAMPLE_FACTORS SU0004256 S00015039 Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004256 S00015040 Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004256 S00015041 Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004256 S00015051 Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium SUBJECT_SAMPLE_FACTORS SU0004256 S00015052 Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium SUBJECT_SAMPLE_FACTORS SU0004256 S00015053 Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium SUBJECT_SAMPLE_FACTORS SU0004257 S00015042 Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004257 S00015043 Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004257 S00015044 Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells SUBJECT_SAMPLE_FACTORS SU0004257 S00015054 Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium SUBJECT_SAMPLE_FACTORS SU0004257 S00015055 Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium SUBJECT_SAMPLE_FACTORS SU0004257 S00015056 Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium #COLLECTION CO:SAMPLE_TYPE Culture Media CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_PROTOCOL_FILENAME Fluxomics_analysis_protocol-2015-03-11.pdf SP:SAMPLEPREP_SUMMARY - #CHROMATOGRAPHY CH:METHODS_ID AQM011 CH:METHODS_FILENAME ALPHA_KETO_ACIDS-FULL.M.zip CH:INSTRUMENT_NAME Agilent 7890A CH:COLUMN_NAME Agilent DB5-MS (30m × 0.25mm, 0.25um) CH:CHROMATOGRAPHY_TYPE GC #ANALYSIS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ANALYSIS_TYPE MS AN:ACQUISITION_PARAMETERS_FILE ALPHA_KETO_ACIDS-FULL.M.zip AN:PROCESSING_PARAMETERS_FILE EX00313-MassHunterQuant-GCMS-DataAnalysis-Method.m.zip #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975C MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Area MS_METABOLITE_DATA_START Samples S00015033 S00015034 S00015035 S00015045 S00015046 S00015047 S00015036 S00015037 S00015038 S00015048 S00015049 S00015050 S00015039 S00015040 S00015041 S00015051 S00015052 S00015053 S00015042 S00015043 S00015044 S00015054 S00015055 S00015056 Factors Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium Treatment:13C glucose labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium Treatment:13C glucose labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:cells Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium Treatment:13C glutamine labeled | Cell type:Cloned Jurkat cell mid level Noxa | Harvest:spent medium Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:cells Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium Treatment:13C glutamine labeled | Cell type:Noxa overexpressed Jurkat cell | Harvest:spent medium Alanine_123 13946.4045 23836.1602 19401.0193 93935.7790 118672.6241 80916.0234 37336.8012 30533.1700 46066.4582 209599.2424 136501.5492 147521.0835 27409.8539 20544.8625 26981.3570 160171.2770 193673.8020 168165.3893 68820.4409 79710.9403 97365.3580 403529.8501 317167.1108 328882.9787 Alanine_123_13C_1 1187.3930 1102.7667 864.8146 2301.2232 2678.9725 1605.2771 1922.9983 1907.0482 1540.2306 6047.6456 4573.8030 5027.0806 610.7947 302.9425 212.1172 1301.0563 1757.7622 13.9795 451.8634 2047.4426 826.6287 Alanine_123_13C_2 Alanine_123_13C_3 alpha-Ketoglutarate_12345 1508.5727 3341.8994 3092.6324 4859.8714 5580.8056 4618.9651 4455.6802 3791.5567 5453.2199 7248.8943 5686.0196 6707.3299 1604.9805 1553.7467 1178.5823 8244.6220 6703.3730 6369.8232 1577.5312 1701.4975 2583.0035 10187.5418 7415.8697 7338.4206 alpha-Ketoglutarate_12345_13C_1 167.8245 330.7430 507.0839 16.9111 178.5766 137.4980 112.3004 337.5795 353.1180 443.8694 251.0503 529.2589 443.5990 591.3201 830.2040 559.6435 alpha-Ketoglutarate_12345_13C_2 542.6538 1197.1422 821.7623 136.0254 116.2931 18.9503 1146.2661 773.2822 1000.5923 267.9292 116.9735 344.8866 413.5972 158.3065 322.0441 259.2203 163.6813 287.7309 366.6106 383.2836 531.8966 52.4851 82.1373 alpha-Ketoglutarate_12345_13C_3 58.8918 136.9976 274.4768 2.6201 217.1847 111.1855 284.1552 65.7375 5.0756 636.2558 668.1958 404.2645 182.9797 82.5358 973.2408 1062.4256 1717.4183 482.1048 305.6500 214.4576 alpha-Ketoglutarate_12345_13C_4 253.9079 166.2030 59.9371 115.4462 0.2333 169.6974 197.2101 18.1431 67.2132 22.5675 alpha-Ketoglutarate_12345_13C_5 40.9824 684.2794 90.3303 1452.1323 1240.2318 1189.6302 636.6531 560.8207 709.5522 3629.9375 3447.5548 5190.7595 2626.1111 2171.4536 1819.2565 Aspartate_1234 8865.6758 17713.1647 15187.1444 228923.9799 241469.3579 192907.7344 35829.4215 28380.8678 44245.2587 279318.6707 210554.8250 214249.6842 8432.3788 6075.6883 7016.5499 229075.8575 250862.3179 222672.9529 14739.0925 12915.1212 21159.4647 285058.4263 243023.6849 267847.1297 Aspartate_1234_13C_1 1265.5740 2646.5842 957.1736 673.4535 2141.6830 1602.2012 2587.3655 127.1043 1089.3883 1602.3666 1314.2888 1373.2348 3316.4609 2677.2777 3811.0582 1866.0427 Aspartate_1234_13C_2 2054.8915 3931.9448 3625.8779 823.0323 7291.1306 5618.8865 8531.1893 618.0064 3.0083 545.7553 2310.8761 1685.0775 1807.9870 310.0623 5619.6726 4548.5582 8530.6191 Aspartate_1234_13C_3 789.7574 2021.3229 1656.9822 213.4386 233.5706 2107.1280 1504.5524 1938.7274 148.3430 290.7345 297.6075 1880.0903 1301.2636 1720.1441 239.3017 384.3082 31.7997 4567.1012 4929.1809 6610.0842 682.7321 886.4394 381.1378 Aspartate_1234_13C_4 275.1227 487.0237 58.2430 233.3778 237.6697 402.0836 324.5742 857.4418 280.3231 3250.5359 2407.0747 2737.7238 389.4073 421.0070 647.7201 11728.1134 10118.1487 16639.2782 1615.6758 1322.3323 1052.8799 Fumarate_1234 13336.8332 26035.5423 21488.7269 43473.3294 47854.0175 28257.2002 38187.7435 31249.8309 47330.1032 49154.4622 33004.4451 46732.6743 12167.9305 9052.9347 8817.4994 43007.9013 49554.2634 54533.3515 16735.0233 20364.6220 22603.7503 58132.4738 48246.3685 41996.3087 Fumarate_1234_13C_1 3140.3321 6917.2047 6488.2241 9027.8703 8603.7895 6160.4511 8435.0647 6332.5911 12936.2307 9422.3364 6570.4067 9313.7605 4167.0608 2433.1030 2662.7839 7170.8713 9011.3688 9369.1861 4711.5371 5976.7164 6305.2392 8505.9599 8816.0708 6966.3537 Fumarate_1234_13C_2 2802.4207 6337.2229 4889.2164 5610.5029 4319.4776 7541.6813 4501.9253 3245.8191 2850.9697 7929.7300 10029.5904 10633.1164 Fumarate_1234_13C_3 1120.3954 2630.2890 2230.8696 194.9683 1.4131 2382.8681 1496.5500 2735.6154 2664.0990 1592.5672 1707.8527 47.1879 4738.0664 5403.7544 6692.2041 561.7848 376.5796 780.4446 Fumarate_1234_13C_4 267.7026 343.5856 599.4219 625.7820 888.4741 310.0452 501.8303 576.5415 535.6923 196.9056 717.1528 7701.2364 5785.9642 5552.9044 3049.4536 4982.6659 5441.3288 22794.6148 27614.7377 29892.4570 15271.9250 12819.6657 11656.0461 Glutamate_12345 246489.1264 447738.1694 374228.1456 244459.9038 316715.0342 175282.9126 453113.8202 370868.6041 543585.5358 319366.7621 274229.9878 260733.8677 136921.6087 113739.6051 122755.6696 210737.7018 256430.4515 207180.1401 95322.7049 109504.9377 163976.8153 244964.5565 213491.3199 215117.8433 Glutamate_12345_13C_1 30366.7754 51708.4397 45875.9433 829.3287 3067.3352 1427.5010 24353.1022 19188.6863 31408.8286 2393.3134 238.3126 1225.4142 43535.1924 33352.8449 39161.9534 3180.8451 4255.0380 812.9258 24080.5543 29246.8095 43949.9078 1414.7946 2366.3220 2311.3463 Glutamate_12345_13C_2 95553.6890 176756.0181 147583.0818 9566.2964 8436.1217 6851.7676 109664.4691 90202.2317 130123.4194 10909.5250 7499.4586 8812.4058 38022.9718 31721.8357 33986.3172 5121.0052 4997.6892 6579.6388 29611.0587 32173.5676 50404.2214 7762.0031 4624.9868 6445.7023 Glutamate_12345_13C_3 25626.8135 46615.6605 37697.8997 1529.5847 2516.2890 46.9756 17654.0107 14213.3392 20765.2267 438.0876 1429.4366 299.9402 75172.9856 62753.3089 68589.5725 9548.8687 10203.9516 8434.0275 70305.4866 79901.5791 121863.5501 15768.6351 16138.2240 14608.7963 Glutamate_12345_13C_4 18630.2254 34213.8538 28574.6164 1127.7890 1875.2843 1175.1829 16455.7402 13518.2326 19147.6358 1325.1717 920.0002 902.6857 10326.7317 7435.3625 9026.3695 2249.6554 3536.1232 1966.8780 11905.0724 11558.1065 18264.1686 5453.3442 4087.5067 4771.9165 Glutamate_12345_13C_5 4546.5052 8101.1072 7068.1055 768.8016 68.2109 284.3126 2680.7288 1818.0909 3062.9588 188.9500 69.3817 241.0291 164746.0745 136332.5389 147746.8291 35169.0583 43711.1547 34343.4403 214787.1253 244589.5404 379396.7731 108397.9149 94321.1923 94499.7841 Glutamine_12345 114126.0438 185008.2466 162519.1118 639346.7193 809099.7158 348246.0067 141275.6844 131914.3357 145348.1160 529971.3499 413222.5527 588356.1418 1922.6820 2059.6107 2754.3016 8239.8288 8894.7434 9255.9415 2327.8929 3060.7149 3575.2938 8073.5460 5995.8298 4057.9931 Glutamine_12345_13C_1 557.1677 1294.9063 1535.4814 1977.6522 1529.4552 1526.6239 2460.9629 4597.8716 582.3357 153.6640 214.1047 2554.3549 1011.5151 189.7123 175.1357 131.4019 410.8362 468.3568 588.1075 Glutamine_12345_13C_2 464.1451 1158.1542 865.9297 1156.3226 2641.0883 50.8426 873.5271 1858.2734 1427.2625 3470.4219 175.2365 274.2351 372.8306 1802.3318 1467.2114 630.3654 117.6247 442.8605 306.4896 854.5984 652.6394 184.2092 Glutamine_12345_13C_3 31.5859 85.1333 152.7619 561.4038 282.3390 59.4555 51.9662 62.2817 64.2597 4178.0397 4143.8223 4938.7258 35510.3660 32540.7291 26678.3717 5258.1927 6535.1802 8280.2297 37891.9022 25778.9866 19836.5459 Glutamine_12345_13C_4 279.4287 395.8159 409.3843 43.1731 580.0975 1283.1838 148.0870 574.3745 108.4205 867.0167 766.8016 885.1215 4544.1689 4559.4715 3817.6228 839.9123 1148.8468 1142.4230 4010.5110 2736.7760 2190.9187 Glutamine_12345_13C_5 12.7842 128.9776 341.8143 134.5207 47.2168 46.7542 82.3288 96961.5622 90203.9701 108599.2483 853167.6794 761931.1655 619301.6836 120278.2706 162582.6506 184897.6799 841990.5200 601974.8192 448494.2167 Lactate_123 725219.4381 1125457.1810 887330.1256 3530902.0450 3958196.8800 3400691.0200 781706.3665 841993.5302 1084129.0190 4463867.3890 3104040.3510 3695792.3520 1849037.8530 1123160.3170 1368668.2940 7518654.8120 8761044.8650 8972091.7820 1404051.3560 1632725.3810 1955677.7430 8867016.9270 7022522.3950 7236436.5580 Lactate_123_13C_1 28989.4119 53759.6992 37820.7590 168401.2944 198383.6980 160784.0351 37478.0835 37591.0980 52543.4327 224079.4054 145514.3387 180609.5041 17439.4879 4754.5633 8045.7973 103699.0746 156422.7712 154094.5918 8853.3145 8825.6492 10312.2579 166930.6224 105082.8853 118433.2398 Lactate_123_13C_2 642759.9466 1000751.3140 790280.8838 3167714.8840 3542252.6000 3021527.9850 675942.1074 723548.3205 927887.2457 3821619.2120 2658738.5110 3180095.1500 8391.4933 5128.2779 6082.1895 49254.3603 62252.0645 65328.6485 10390.5767 12441.3835 14026.8890 83727.9199 64967.4942 67078.5756 Lactate_123_13C_3 5578.4351 8406.3631 10058.4983 40940.9322 50728.8402 40631.3294 8184.1334 9971.5997 14634.9772 59296.7378 39022.6138 44984.5800 16654.1072 9789.0399 12200.7587 69727.0041 80607.4835 80137.4734 27574.6681 30024.8161 38736.8896 189321.4044 149098.1265 148200.9497 Oxaloacetate_1234 2349.7664 3734.0129 3675.7301 3350.2378 1626.6849 Oxaloacetate_1234_13C_1 8.5507 Oxaloacetate_1234_13C_2 47.1355 33.2939 Oxaloacetate_1234_13C_3 Oxaloacetate_1234_13C_4 Pyruvate_123 3731.2106 8283.3021 5322.2136 24476.9755 43515.3062 28621.6516 8686.8653 9246.8136 13700.2271 129746.3356 81380.3214 143748.1353 12015.6419 7107.3001 8776.0884 79441.4531 75621.2595 85330.0551 18075.4108 19228.1593 23930.8681 336230.4273 297917.9943 213369.3199 Pyruvate_123_13C_1 182.5224 320.2651 65.4932 1462.0779 2055.4473 1859.7561 458.6734 516.9776 713.8376 5184.2214 3241.9108 6107.8836 294.6852 183.8513 118.3093 934.8745 809.3345 1115.3012 233.5793 289.0531 1252.8495 2409.1501 1320.6166 Pyruvate_123_13C_2 2912.5800 6943.1454 3916.3589 18899.0763 30695.2221 18365.2875 7837.8984 7803.0428 10861.4319 104024.8914 66340.6579 117924.1762 334.9233 33.4237 277.2314 504.4932 747.6122 590.0706 451.3456 363.9825 843.9647 2649.1839 2900.9303 2022.9872 Pyruvate_123_13C_3 2.8352 38.6704 73.7118 91.2297 131.9578 201.2126 104.6286 19.3775 35.5049 1205.1657 886.4305 1172.3386 136.7999 1.9437 70.4009 653.8702 854.0217 807.1240 309.7415 453.2105 484.0140 6487.4529 5768.4310 4470.8118 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant Alanine_123 5950 C00041 ALA_123 UM_Target_ID Alanine_123_13C_1 5950 C00041 ALA_123_13C_1 UM_Target_ID Alanine_123_13C_2 5950 C00041 ALA_123_13C_2 UM_Target_ID Alanine_123_13C_3 5950 C00041 ALA_123_13C_3 UM_Target_ID alpha-Ketoglutarate_12345 51 C00026 AKG_12345 UM_Target_ID alpha-Ketoglutarate_12345_13C_1 51 C00026 AKG_12345_13C_1 UM_Target_ID alpha-Ketoglutarate_12345_13C_2 51 C00026 AKG_12345_13C_2 UM_Target_ID alpha-Ketoglutarate_12345_13C_3 51 C00026 AKG_12345_13C_3 UM_Target_ID alpha-Ketoglutarate_12345_13C_4 51 C00026 AKG_12345_13C_4 UM_Target_ID alpha-Ketoglutarate_12345_13C_5 51 C00026 AKG_12345_13C_5 UM_Target_ID Aspartate_1234 5960 C00049 ASP_1234 UM_Target_ID Aspartate_1234_13C_1 5960 C00049 ASP_1234_13C_1 UM_Target_ID Aspartate_1234_13C_2 5960 C00049 ASP_1234_13C_2 UM_Target_ID Aspartate_1234_13C_3 5960 C00049 ASP_1234_13C_3 UM_Target_ID Aspartate_1234_13C_4 5960 C00049 ASP_1234_13C_4 UM_Target_ID Fumarate_1234 444972 C00122 FUM_1234 UM_Target_ID Fumarate_1234_13C_1 444972 C00122 FUM_1234_13C_1 UM_Target_ID Fumarate_1234_13C_2 444972 C00122 FUM_1234_13C_2 UM_Target_ID Fumarate_1234_13C_3 444972 C00122 FUM_1234_13C_3 UM_Target_ID Fumarate_1234_13C_4 444972 C00122 FUM_1234_13C_4 UM_Target_ID Glutamate_12345 33032 GLU_12345 UM_Target_ID Glutamate_12345_13C_1 33032 GLU_12345_13C_1 UM_Target_ID Glutamate_12345_13C_2 33032 GLU_12345_13C_2 UM_Target_ID Glutamate_12345_13C_3 33032 GLU_12345_13C_3 UM_Target_ID Glutamate_12345_13C_4 33032 GLU_12345_13C_4 UM_Target_ID Glutamate_12345_13C_5 33032 GLU_12345_13C_5 UM_Target_ID Glutamine_12345 5961 C00064 GLN_12345 UM_Target_ID Glutamine_12345_13C_1 5961 C00064 GLN_12345_13C_1 UM_Target_ID Glutamine_12345_13C_2 5961 C00064 GLN_12345_13C_2 UM_Target_ID Glutamine_12345_13C_3 5961 C00064 GLN_12345_13C_3 UM_Target_ID Glutamine_12345_13C_4 5961 C00064 GLN_12345_13C_4 UM_Target_ID Glutamine_12345_13C_5 5961 C00064 GLN_12345_13C_5 UM_Target_ID Lactate_123 107689 C00186 LAC_123 UM_Target_ID Lactate_123_13C_1 107689 C00186 LAC_123_13C_1 UM_Target_ID Lactate_123_13C_2 107689 C00186 LAC_123_13C_2 UM_Target_ID Lactate_123_13C_3 107689 C00186 LAC_123_13C_3 UM_Target_ID Oxaloacetate_1234 970 C00036 OAA_1234 UM_Target_ID Oxaloacetate_1234_13C_1 970 C00036 OAA_1234_13C_1 UM_Target_ID Oxaloacetate_1234_13C_2 970 C00036 OAA_1234_13C_2 UM_Target_ID Oxaloacetate_1234_13C_3 970 C00036 OAA_1234_13C_3 UM_Target_ID Oxaloacetate_1234_13C_4 970 C00036 OAA_1234_13C_4 UM_Target_ID Pyruvate_123 1060 C00022 PYR_123 UM_Target_ID Pyruvate_123_13C_1 1060 C00022 PYR_123_13C_1 UM_Target_ID Pyruvate_123_13C_2 1060 C00022 PYR_123_13C_2 UM_Target_ID Pyruvate_123_13C_3 1060 C00022 PYR_123_13C_3 UM_Target_ID METABOLITES_END #END