#METABOLOMICS WORKBENCH araskind_20150608_9705991_mwtab.txt DATATRACK_ID:290 STUDY_ID:ST000191 ANALYSIS_ID:AN000293 PROJECT_ID:PR000162 VERSION 1 CREATED_ON 2016-09-17 #PROJECT PR:PROJECT_TITLE Neurogenic Hypertension PR:PROJECT_SUMMARY Role of neuroinflammation and microbiome in drug-resistant hypertension PR:INSTITUTE University of Florida PR:DEPARTMENT Physiology & Functional Genomics PR:LABORATORY Raizada Lab PR:LAST_NAME Raizada PR:FIRST_NAME Mohan PR:ADDRESS Gainesville, FL PR:EMAIL mraizada@ufl.edu PR:PHONE 352-392-9299 #STUDY ST:STUDY_TITLE SCFA in Hypertension ST:STUDY_TYPE Samples 4-34 are sera and feces from an experiment to determine the effects of ST:STUDY_TYPE on SCFA in Ang II-induced hypertension. Samples 35-59 are plasma samples fron ST:STUDY_TYPE experiment to determine the effects of ACE2 activator, DIZE on SCFA in ST:STUDY_TYPE pulmonary hypertension. Plasma samples were collected using heparin ST:STUDY_SUMMARY Samples 4-34 are sera and feces from an experiment to determine the effects of ST:STUDY_SUMMARY on SCFA in Ang II-induced hypertension. Samples 35-59 are plasma samples fron ST:STUDY_SUMMARY experiment to determine the effects of ACE2 activator, DIZE on SCFA in ST:STUDY_SUMMARY pulmonary hypertension. Plasma samples were collected using heparin ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@umich.edu ST:PHONE - ST:NUM_GROUPS 7 ST:TOTAL_SUBJECTS 56 #SUBJECT SU:SUBJECT_TYPE - SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10114 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0004856 S00015896 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015897 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015898 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015899 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015912 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015913 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015914 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015915 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015928 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015929 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015930 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015931 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015932 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004856 S00015933 Ang II:N | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004862 S00015946 Ang II:N | Mino:N | MCT:N | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004862 S00015947 Ang II:N | Mino:N | MCT:N | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004862 S00015948 Ang II:N | Mino:N | MCT:N | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004862 S00015949 Ang II:N | Mino:N | MCT:N | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004862 S00015950 Ang II:N | Mino:N | MCT:N | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004862 S00015951 Ang II:N | Mino:N | MCT:N | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004860 S00015934 Ang II:N | Mino:N | MCT:Y | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004860 S00015935 Ang II:N | Mino:N | MCT:Y | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004860 S00015936 Ang II:N | Mino:N | MCT:Y | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004860 S00015937 Ang II:N | Mino:N | MCT:Y | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004860 S00015938 Ang II:N | Mino:N | MCT:Y | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004860 S00015939 Ang II:N | Mino:N | MCT:Y | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004861 S00015940 Ang II:N | Mino:N | MCT:Y | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004861 S00015941 Ang II:N | Mino:N | MCT:Y | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004861 S00015942 Ang II:N | Mino:N | MCT:Y | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004861 S00015943 Ang II:N | Mino:N | MCT:Y | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004861 S00015944 Ang II:N | Mino:N | MCT:Y | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004861 S00015945 Ang II:N | Mino:N | MCT:Y | DIZE:Y SUBJECT_SAMPLE_FACTORS SU0004859 S00015908 Ang II:N | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004859 S00015909 Ang II:N | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004859 S00015910 Ang II:N | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004859 S00015911 Ang II:N | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004859 S00015924 Ang II:N | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004859 S00015925 Ang II:N | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004859 S00015926 Ang II:N | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004859 S00015927 Ang II:N | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004857 S00015900 Ang II:Y | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004857 S00015901 Ang II:Y | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004857 S00015902 Ang II:Y | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004857 S00015903 Ang II:Y | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004857 S00015916 Ang II:Y | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004857 S00015917 Ang II:Y | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004857 S00015918 Ang II:Y | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004857 S00015919 Ang II:Y | Mino:N | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004858 S00015904 Ang II:Y | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004858 S00015905 Ang II:Y | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004858 S00015906 Ang II:Y | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004858 S00015907 Ang II:Y | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004858 S00015920 Ang II:Y | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004858 S00015921 Ang II:Y | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004858 S00015922 Ang II:Y | Mino:Y | MCT:N | DIZE:N SUBJECT_SAMPLE_FACTORS SU0004858 S00015923 Ang II:Y | Mino:Y | MCT:N | DIZE:N #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Serum #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME SCFA_analysis_protocol-2015-03-10.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME - CH:COLUMN_NAME Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um) CH:METHODS_ID AQM030 CH:METHODS_FILENAME WAXPLUS_100-200C_2_SIM.txt #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ACQUISITION_PARAMETERS_FILE WAXPLUS_100-200C_2_SIM.txt AN:PROCESSING_PARAMETERS_FILE EX00350-SCFA-Quant-methods-plasma.m.zip #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975C MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/mg sample MS_METABOLITE_DATA_START Samples S00015912 S00015913 S00015914 S00015915 S00015924 S00015925 S00015926 S00015927 S00015916 S00015917 S00015918 S00015919 S00015920 S00015921 S00015922 S00015923 Factors Ang II:N | Mino:N | MCT:N | DIZE:N Ang II:N | Mino:N | MCT:N | DIZE:N Ang II:N | Mino:N | MCT:N | DIZE:N Ang II:N | Mino:N | MCT:N | DIZE:N Ang II:N | Mino:Y | MCT:N | DIZE:N Ang II:N | Mino:Y | MCT:N | DIZE:N Ang II:N | Mino:Y | MCT:N | DIZE:N Ang II:N | Mino:Y | MCT:N | DIZE:N Ang II:Y | Mino:N | MCT:N | DIZE:N Ang II:Y | Mino:N | MCT:N | DIZE:N Ang II:Y | Mino:N | MCT:N | DIZE:N Ang II:Y | Mino:N | MCT:N | DIZE:N Ang II:Y | Mino:Y | MCT:N | DIZE:N Ang II:Y | Mino:Y | MCT:N | DIZE:N Ang II:Y | Mino:Y | MCT:N | DIZE:N Ang II:Y | Mino:Y | MCT:N | DIZE:N aceticacid 9670.4680 27676.4560 10650.6960 12842.0180 6834.3830 3483.8260 135.7560 551.9060 9281.4160 9142.5730 16704.1060 7789.5520 9440.9310 7754.2400 10059.1710 14831.4040 butyricacid 9147.4690 9043.4340 17616.4000 21983.3100 4670.5050 18116.9990 2905.5530 1475.3960 16392.4080 5807.7600 9913.1420 12044.0480 1802.1990 8620.9060 11003.2970 10452.5680 hexanoicacid 21.8680 21.6780 22.7170 1420.2370 14.7520 15.5250 10.8720 5.8760 50.0710 19.5920 425.9030 21.0250 0.0000 20.2700 39.8100 23.9030 iso-butyricacid 533.6300 300.6870 467.5590 807.1930 400.0370 786.3830 215.5700 157.8210 443.0000 449.6510 495.6440 264.0620 55.5200 272.9260 723.5280 758.1930 isovalericacid 615.4020 196.1300 486.1400 763.8810 379.5920 674.7490 221.3150 143.7320 351.7400 380.2220 231.5830 264.2630 58.4280 272.2000 510.5560 697.8440 propionic acid 3431.9930 5560.5580 4874.8990 4072.0130 3724.2510 7094.9150 3371.1580 3474.9910 3788.1600 2035.7540 5337.4930 1990.9590 2682.4610 3069.4800 3820.8580 4101.4260 valericacid 1748.2140 370.6530 1534.3530 1808.3970 993.1450 2747.0810 726.0250 185.1560 475.4910 959.9700 889.0730 1169.0280 421.7970 1129.5980 1109.5650 1907.0970 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type aceticacid 176 Acetate UM_Target_ID butyricacid 20975673 Butyrate UM_Target_ID hexanoicacid 8892 Hexanoate UM_Target_ID iso-butyricacid 6590 iso-butyrate UM_Target_ID isovalericacid 10430 Isovalerate UM_Target_ID propionic acid 1032 Propionate UM_Target_ID valericacid 7991 Valerate UM_Target_ID METABOLITES_END #END