#METABOLOMICS WORKBENCH STUDY_ID:ST000198 ANALYSIS_ID:AN000300 PROJECT_ID:PR000169
VERSION             	1
CREATED_ON          	2016-09-17
#PROJECT
PR:PROJECT_TITLE                 	Regulation of hepatic metabolism
PR:PROJECT_SUMMARY               	Examine metabolite in liver and plasma before and after 13C-glucose load in
PR:PROJECT_SUMMARY               	type, LIRKO and LIRFKO mice
PR:INSTITUTE                     	University of Chicago
PR:DEPARTMENT                    	Jesse Brown VA Medical Center
PR:LABORATORY                    	Unterman Lab
PR:LAST_NAME                     	Unterman
PR:FIRST_NAME                    	Terry
PR:ADDRESS                       	Chicago, IL
PR:EMAIL                         	unterman@uic.edu
PR:PHONE                         	847-778-0943
#STUDY
ST:STUDY_TITLE                   	Liver and Plasma metaboites for 13C-glucose load in wild type, LIRKO and LIRFKO
ST:STUDY_SUMMARY                 	Mice were fasted for 18 hr overnight then sacrificed or treated with
ST:STUDY_SUMMARY                 	(2 g/kg ip) and sacrificed 1 hr later by decapitation and liver was immediately
ST:STUDY_SUMMARY                 	and stored in liquid N2 and then at -80 C. Wild type (IR and IR/FoxO1 floxed)
ST:STUDY_SUMMARY                 	were sacrificed after fasting and 1 hr post-glucose treatment. Liver-specific
ST:STUDY_SUMMARY                 	receptor knockout (LIRKO) and insulin receptor/FoxO1 double knockout (LIRFKO)
ST:STUDY_SUMMARY                 	were sacrificed 1 hr post glucose treatment
ST:INSTITUTE                     	University of Michigan
ST:DEPARTMENT                    	Biomedical Research Core Facilities
ST:LABORATORY                    	Metabolomics core
ST:LAST_NAME                     	Kachman
ST:FIRST_NAME                    	Maureen
ST:ADDRESS                       	6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL                         	mkachman@umich.edu
ST:PHONE                         	-
ST:NUM_GROUPS                    	5
ST:TOTAL_SUBJECTS                	40
#SUBJECT
SU:SUBJECT_TYPE                  	-
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	SU0003002	S00013145	treatment:glucose | Strain:IRF floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003002	S00013165	treatment:glucose | Strain:IRF floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003001	S00013142	treatment:glucose | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003001	S00013143	treatment:glucose | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003001	S00013144	treatment:glucose | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003001	S00013162	treatment:glucose | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003001	S00013163	treatment:glucose | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003001	S00013164	treatment:glucose | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003004	S00013152	treatment:glucose | Strain:LIRFKO	
SUBJECT_SAMPLE_FACTORS           	SU0003004	S00013153	treatment:glucose | Strain:LIRFKO	
SUBJECT_SAMPLE_FACTORS           	SU0003004	S00013154	treatment:glucose | Strain:LIRFKO	
SUBJECT_SAMPLE_FACTORS           	SU0003004	S00013155	treatment:glucose | Strain:LIRFKO	
SUBJECT_SAMPLE_FACTORS           	SU0003004	S00013172	treatment:glucose | Strain:LIRFKO	
SUBJECT_SAMPLE_FACTORS           	SU0003004	S00013173	treatment:glucose | Strain:LIRFKO	
SUBJECT_SAMPLE_FACTORS           	SU0003004	S00013174	treatment:glucose | Strain:LIRFKO	
SUBJECT_SAMPLE_FACTORS           	SU0003004	S00013175	treatment:glucose | Strain:LIRFKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013146	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013147	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013148	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013149	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013150	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013151	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013166	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013167	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013168	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013169	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013170	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003003	S00013171	treatment:glucose | Strain:LIRKO	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013136	treatment:none | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013137	treatment:none | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013138	treatment:none | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013139	treatment:none | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013140	treatment:none | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013141	treatment:none | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013156	treatment:none | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013157	treatment:none | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013158	treatment:none | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013159	treatment:none | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013160	treatment:none | Strain:IR floxed	
SUBJECT_SAMPLE_FACTORS           	SU0003000	S00013161	treatment:none | Strain:IR floxed	
#COLLECTION
CO:COLLECTION_SUMMARY            	-
CO:SAMPLE_TYPE                   	Liver
#TREATMENT
TR:TREATMENT_SUMMARY             	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	-
SP:SAMPLEPREP_PROTOCOL_FILENAME  	Acyl-carnitines_analysis_protocol-CR-MK-20150528.docx
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1200
CH:COLUMN_NAME                   	Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
CH:METHODS_ID                    	AQM050
CH:METHODS_FILENAME              	QM-004-Xbridg2mm_ACar+_MRM-Insert.m.zip
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	MRC2 (University of Michigan)
AN:ACQUISITION_PARAMETERS_FILE   	QM-004-Xbridg2mm_ACar+_MRM-Insert.m.zip
AN:PROCESSING_PARAMETERS_FILE    	EX00258-acyl-carnitines-quant.m.zip
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Agilent 6490 QQQ
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	pmol/mg sample
MS_METABOLITE_DATA_START
Samples	S00013145	S00013142	S00013143	S00013144	S00013152	S00013153	S00013154	S00013155	S00013146	S00013147	S00013148	S00013149	S00013150	S00013151	S00013136	S00013137	S00013138	S00013139	S00013140	S00013141
Factors	treatment:glucose | Strain:IRF floxed	treatment:glucose | Strain:IR floxed	treatment:glucose | Strain:IR floxed	treatment:glucose | Strain:IR floxed	treatment:glucose | Strain:LIRFKO	treatment:glucose | Strain:LIRFKO	treatment:glucose | Strain:LIRFKO	treatment:glucose | Strain:LIRFKO	treatment:glucose | Strain:LIRKO	treatment:glucose | Strain:LIRKO	treatment:glucose | Strain:LIRKO	treatment:glucose | Strain:LIRKO	treatment:glucose | Strain:LIRKO	treatment:glucose | Strain:LIRKO	treatment:none | Strain:IR floxed	treatment:none | Strain:IR floxed	treatment:none | Strain:IR floxed	treatment:none | Strain:IR floxed	treatment:none | Strain:IR floxed	treatment:none | Strain:IR floxed
3-hydroxylauroylcarnitine	0.1521	0.0213	0.2310	0.1098	0.0146	0.3919	0.1186	0.0773	0.1376	0.0718	0.0841	0.1423	0.2572	0.2879	0.2668	0.1449	0.1200	0.0499	0.0688	0.0547
3-Hydroxylinoleoylcarnitine	1.2081	0.1451	1.1931	1.2044	0.2009	1.5779	0.7552	0.7989	1.3452	0.5402	0.3241	0.5685	1.1870	1.4747	1.0691	0.9224	1.2929	0.3713	0.6906	0.4701
3-hydroxymyristoylcarnitine	0.2985	0.0488	0.2943	0.1753	0.0302	0.5416	0.1828	0.1470	0.2756	0.1095	0.1303	0.2295	0.5018	0.4196	0.3827	0.2448	0.2380	0.0796	0.1196	0.1245
3-Hydroxypalmitoylcarnitine	0.9626	0.1801	0.8344	0.7746	0.1094	1.2698	0.6540	0.4887	0.8584	0.3285	0.3388	0.5307	1.0523	0.9804	0.9799	0.7892	1.1370	0.2626	0.5127	0.3727
Acetylcarnitine	460.1183	103.3001	579.0372	778.6006	77.3024	474.7368	526.6616	100.4459	368.4689	72.8757	145.1588	176.4865	222.8464	317.0617	153.8084	602.4624	504.1044	97.8500	189.0036	79.4457
arachidonoylcarnitine	0.6088	0.5631	0.6280	1.0802	0.1530	0.7793	0.7063	0.1364	0.5330	0.1658	0.4069	0.2447	0.1269	0.5925	0.5434	0.7071	0.6833	0.1458	0.2440	0.0787
butyrylcarnitine	1.0532	0.7442	1.1539	1.6544	0.7058	1.3291	1.2807	0.6666	1.0524	0.6182	0.6873	0.7582	0.6773	1.2867	1.0101	1.0650	1.1424	0.6053	0.6829	0.2800
Decanoylcarnitine	0.4040	0.1970	2.2558	0.5719	0.0769	2.1254	0.6938	0.2263	0.4452	0.3574	0.1413	0.0899	0.0667	1.1516	1.0160	0.9990	0.9088	0.2005	0.2644	0.1113
decenoylcarnitine	0.0881	0.0832	0.9073	0.1186	0.0088	0.4649	0.1635	0.0329	0.0650	0.0450	0.0135	0.0072	0.0102	0.1364	0.3854	0.2248	0.2290	0.0217	0.0398	0.0208
Dodecenoylcarnitine	0.1910	0.0819	0.6763	0.1248	0.0158	0.8821	0.2507	0.1267	0.0779	0.2204	0.0248	0.0240	0.0231	0.3873	0.5884	0.4017	0.2522	0.0917	0.0931	0.0719
Glutarylcarnitine	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000
Hexanoylcarnitine	0.2840	0.1943	0.5967	0.7129	0.1474	0.6697	0.5254	0.1667	0.3287	0.2419	0.0611	0.0543	0.0372	0.6769	0.8870	0.6051	0.4378	0.1880	0.2727	0.1293
icosadienoylcarnitine	0.5214	0.3012	0.8343	1.0329	0.1650	0.9940	0.5083	0.2399	0.3469	0.2398	0.0562	0.0677	0.0550	0.5345	0.6858	0.8564	0.5815	0.1181	0.2175	0.0475
Icosanoylcarnitine	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000
icosatrienoylcarnitine	0.0944	0.0772	0.1276	0.2224	0.0228	0.1781	0.0848	0.0492	0.0472	0.0417	0.0105	0.0105	0.0066	0.0696	0.0947	0.1484	0.0934	0.0186	0.0279	0.0092
icoseneoylcarnitine	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000
isovalerylcarnitine	1.1371	0.9347	1.4548	2.8231	0.9664	1.4876	2.0545	0.3015	1.9744	0.3616	0.7420	0.9882	0.7821	1.5630	1.2236	1.3660	1.5534	0.3730	0.5316	0.1358
lauroylcarnitine	0.4107	0.2401	0.7317	0.4193	0.0576	1.0009	0.5135	0.3418	0.2975	0.5073	0.0871	0.0651	0.0654	0.7683	1.0217	0.6840	0.6331	0.2549	0.3293	0.2037
L-Carnitine	299.6038	125.5179	385.8503	777.3591	107.7137	441.4766	442.8754	80.3070	698.7451	55.0813	687.1565	734.0856	495.2323	484.0477	437.8671	387.2361	353.1963	78.6520	195.0307	53.6860
Linoelaidyl carnitine	2.1025	1.3334	2.5223	2.5717	0.7035	2.6919	1.8427	1.6663	1.6570	1.9490	0.6404	0.6551	0.4212	1.9070	3.7611	2.9335	3.0581	1.0218	1.8559	0.8984
myristoylcarnitine	1.0495	0.7300	1.3978	1.1850	0.2725	1.9070	1.1672	1.0760	0.9343	1.1827	0.3413	0.3627	0.3649	1.5504	2.1358	1.3872	1.4124	0.6049	0.9604	0.7191
octanoylcarnitine	0.2112	0.1088	0.4179	0.3031	0.0618	0.5203	0.2297	0.1366	0.2257	0.1533	0.0598	0.0398	0.0259	0.4676	0.7356	0.4736	0.2172	0.1163	0.1506	0.0695
Octenoylcarnitine	0.0255	0.0390	0.0597	0.0303	0.0031	0.0439	0.0303	0.0082	0.0113	0.0084	0.0033	0.0032	0.0041	0.0242	0.0869	0.0445	0.0355	0.0059	0.0103	0.0056
Oleoylcarnitine	2.7247	1.7331	3.1415	3.4424	1.2356	4.1077	2.7438	2.3134	2.8587	3.0086	0.9557	1.1285	1.0040	3.6295	4.4440	3.2500	3.7377	1.5416	2.5356	1.2719
Palmitoleylcarnitine	1.9973	0.9657	2.3187	1.7240	0.3673	3.2745	2.0186	1.8254	1.7849	2.2015	0.3837	0.3728	0.3608	2.6316	3.0896	2.3783	2.5067	0.9053	1.5741	0.9288
palmitoylcarnitine	2.6032	2.0009	2.9087	3.1859	1.2036	4.0392	2.9591	2.3876	2.6473	2.7571	1.1606	1.3258	1.2453	3.4065	4.6390	3.2950	3.8190	1.5069	2.7191	1.5694
propionylcarnitine	1.9865	3.9293	2.3617	3.0066	4.6204	2.9391	1.5886	2.7709	2.0578	2.7120	2.8174	3.9937	4.0633	2.2433	9.9879	9.4323	12.7799	4.0108	8.6229	2.6676
Stearoylcarnitine	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000
Tetradecadiencarnitine	0.3841	0.1217	0.7502	0.3469	0.0287	0.7730	0.3641	0.1866	0.1058	0.2308	0.0264	0.0212	0.0190	0.3341	0.7790	0.6055	0.5682	0.1417	0.2193	0.1177
Tetradecenoylcarnitine	1.1395	0.4289	1.6890	0.9608	0.1552	2.2763	1.2573	0.9177	0.6881	1.1458	0.1192	0.1352	0.1225	1.6132	1.8721	1.3564	1.4398	0.6288	0.7097	0.5458
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	moverz_quant	ri	ri_type	pubchem_id	inchi_key	kegg_id	other_id	other_id_type
3-hydroxylauroylcarnitine				71464535			C12-OH	UM_Target_ID
3-Hydroxylinoleoylcarnitine				78076335			C18:2-OH	UM_Target_ID
3-hydroxymyristoylcarnitine				71464541			C14-OH	UM_Target_ID
3-Hydroxypalmitoylcarnitine				71464553			C16-OH	UM_Target_ID
Acetylcarnitine				1			C2	UM_Target_ID
arachidonoylcarnitine				71464486			C20:4	UM_Target_ID
butyrylcarnitine				439829			C4	UM_Target_ID
Decanoylcarnitine				3332882			C10:0	UM_Target_ID
decenoylcarnitine				54074509			C10:1	UM_Target_ID
Dodecenoylcarnitine				53481671			C12:1	UM_Target_ID
Glutarylcarnitine				71317118			C5:0-DC	UM_Target_ID
Hexanoylcarnitine				6426853			C6:0	UM_Target_ID
icosadienoylcarnitine				163425699			C20:2	UM_Target_ID
Icosanoylcarnitine				53477833			C20	UM_Target_ID
icosatrienoylcarnitine							C20:3	UM_Target_ID
icoseneoylcarnitine				71464507			C20:1	UM_Target_ID
isovalerylcarnitine				6426852			C5:0	UM_Target_ID
lauroylcarnitine				168381			C12:0	UM_Target_ID
L-Carnitine				10917			L-Carnitine	UM_Target_ID
Linoelaidyl carnitine				53477834			C18:2	UM_Target_ID
myristoylcarnitine				3413344			C14:0	UM_Target_ID
octanoylcarnitine				123701			C8:0	UM_Target_ID
Octenoylcarnitine				71464472			C8:1	UM_Target_ID
Oleoylcarnitine				166952			C18:1	UM_Target_ID
Palmitoleylcarnitine				76040365			C16:1	UM_Target_ID
palmitoylcarnitine				461			C16:0	UM_Target_ID
propionylcarnitine				107738			C3	UM_Target_ID
Stearoylcarnitine				5282			C18:0	UM_Target_ID
Tetradecadiencarnitine				53481681			C14:2	UM_Target_ID
Tetradecenoylcarnitine				22833575			C14:1	UM_Target_ID
METABOLITES_END
#END