#METABOLOMICS WORKBENCH araskind_20150610_9777851_mwtab.txt DATATRACK_ID:299 STUDY_ID:ST000198 ANALYSIS_ID:AN000300 PROJECT_ID:PR000169 VERSION 1 CREATED_ON 2016-09-17 #PROJECT PR:PROJECT_TITLE Regulation of hepatic metabolism PR:PROJECT_SUMMARY Examine metabolite in liver and plasma before and after 13C-glucose load in PR:PROJECT_SUMMARY type, LIRKO and LIRFKO mice PR:INSTITUTE University of Chicago PR:DEPARTMENT Jesse Brown VA Medical Center PR:LABORATORY Unterman Lab PR:LAST_NAME Unterman PR:FIRST_NAME Terry PR:ADDRESS Chicago, IL PR:EMAIL unterman@uic.edu PR:PHONE 847-778-0943 #STUDY ST:STUDY_TITLE Liver and Plasma metaboites for 13C-glucose load in wild type, LIRKO and LIRFKO ST:STUDY_SUMMARY Mice were fasted for 18 hr overnight then sacrificed or treated with ST:STUDY_SUMMARY (2 g/kg ip) and sacrificed 1 hr later by decapitation and liver was immediately ST:STUDY_SUMMARY and stored in liquid N2 and then at -80 C. Wild type (IR and IR/FoxO1 floxed) ST:STUDY_SUMMARY were sacrificed after fasting and 1 hr post-glucose treatment. Liver-specific ST:STUDY_SUMMARY receptor knockout (LIRKO) and insulin receptor/FoxO1 double knockout (LIRFKO) ST:STUDY_SUMMARY were sacrificed 1 hr post glucose treatment ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@umich.edu ST:PHONE - ST:NUM_GROUPS 5 ST:TOTAL_SUBJECTS 40 #SUBJECT SU:SUBJECT_TYPE - SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0003002 S00013145 treatment:glucose | Strain:IRF floxed SUBJECT_SAMPLE_FACTORS SU0003002 S00013165 treatment:glucose | Strain:IRF floxed SUBJECT_SAMPLE_FACTORS SU0003001 S00013142 treatment:glucose | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003001 S00013143 treatment:glucose | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003001 S00013144 treatment:glucose | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003001 S00013162 treatment:glucose | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003001 S00013163 treatment:glucose | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003001 S00013164 treatment:glucose | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003004 S00013152 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003004 S00013153 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003004 S00013154 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003004 S00013155 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003004 S00013172 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003004 S00013173 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003004 S00013174 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003004 S00013175 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013146 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013147 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013148 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013149 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013150 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013151 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013166 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013167 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013168 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013169 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013170 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013171 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003000 S00013136 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013137 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013138 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013139 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013140 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013141 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013156 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013157 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013158 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013159 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013160 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013161 treatment:none | Strain:IR floxed #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Liver #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME Acyl-carnitines_analysis_protocol-CR-MK-20150528.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1200 CH:COLUMN_NAME Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) CH:METHODS_ID AQM050 CH:METHODS_FILENAME QM-004-Xbridg2mm_ACar+_MRM-Insert.m.zip #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ACQUISITION_PARAMETERS_FILE QM-004-Xbridg2mm_ACar+_MRM-Insert.m.zip AN:PROCESSING_PARAMETERS_FILE EX00258-acyl-carnitines-quant.m.zip #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6490 QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/mg sample MS_METABOLITE_DATA_START Samples S00013145 S00013142 S00013143 S00013144 S00013152 S00013153 S00013154 S00013155 S00013146 S00013147 S00013148 S00013149 S00013150 S00013151 S00013136 S00013137 S00013138 S00013139 S00013140 S00013141 Factors treatment:glucose | Strain:IRF floxed treatment:glucose | Strain:IR floxed treatment:glucose | Strain:IR floxed treatment:glucose | Strain:IR floxed treatment:glucose | Strain:LIRFKO treatment:glucose | Strain:LIRFKO treatment:glucose | Strain:LIRFKO treatment:glucose | Strain:LIRFKO treatment:glucose | Strain:LIRKO treatment:glucose | Strain:LIRKO treatment:glucose | Strain:LIRKO treatment:glucose | Strain:LIRKO treatment:glucose | Strain:LIRKO treatment:glucose | Strain:LIRKO treatment:none | Strain:IR floxed treatment:none | Strain:IR floxed treatment:none | Strain:IR floxed treatment:none | Strain:IR floxed treatment:none | Strain:IR floxed treatment:none | Strain:IR floxed 3-hydroxylauroylcarnitine 0.1521 0.0213 0.2310 0.1098 0.0146 0.3919 0.1186 0.0773 0.1376 0.0718 0.0841 0.1423 0.2572 0.2879 0.2668 0.1449 0.1200 0.0499 0.0688 0.0547 3-Hydroxylinoleoylcarnitine 1.2081 0.1451 1.1931 1.2044 0.2009 1.5779 0.7552 0.7989 1.3452 0.5402 0.3241 0.5685 1.1870 1.4747 1.0691 0.9224 1.2929 0.3713 0.6906 0.4701 3-hydroxymyristoylcarnitine 0.2985 0.0488 0.2943 0.1753 0.0302 0.5416 0.1828 0.1470 0.2756 0.1095 0.1303 0.2295 0.5018 0.4196 0.3827 0.2448 0.2380 0.0796 0.1196 0.1245 3-Hydroxypalmitoylcarnitine 0.9626 0.1801 0.8344 0.7746 0.1094 1.2698 0.6540 0.4887 0.8584 0.3285 0.3388 0.5307 1.0523 0.9804 0.9799 0.7892 1.1370 0.2626 0.5127 0.3727 Acetylcarnitine 460.1183 103.3001 579.0372 778.6006 77.3024 474.7368 526.6616 100.4459 368.4689 72.8757 145.1588 176.4865 222.8464 317.0617 153.8084 602.4624 504.1044 97.8500 189.0036 79.4457 arachidonoylcarnitine 0.6088 0.5631 0.6280 1.0802 0.1530 0.7793 0.7063 0.1364 0.5330 0.1658 0.4069 0.2447 0.1269 0.5925 0.5434 0.7071 0.6833 0.1458 0.2440 0.0787 butyrylcarnitine 1.0532 0.7442 1.1539 1.6544 0.7058 1.3291 1.2807 0.6666 1.0524 0.6182 0.6873 0.7582 0.6773 1.2867 1.0101 1.0650 1.1424 0.6053 0.6829 0.2800 Decanoylcarnitine 0.4040 0.1970 2.2558 0.5719 0.0769 2.1254 0.6938 0.2263 0.4452 0.3574 0.1413 0.0899 0.0667 1.1516 1.0160 0.9990 0.9088 0.2005 0.2644 0.1113 decenoylcarnitine 0.0881 0.0832 0.9073 0.1186 0.0088 0.4649 0.1635 0.0329 0.0650 0.0450 0.0135 0.0072 0.0102 0.1364 0.3854 0.2248 0.2290 0.0217 0.0398 0.0208 Dodecenoylcarnitine 0.1910 0.0819 0.6763 0.1248 0.0158 0.8821 0.2507 0.1267 0.0779 0.2204 0.0248 0.0240 0.0231 0.3873 0.5884 0.4017 0.2522 0.0917 0.0931 0.0719 Glutarylcarnitine 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 Hexanoylcarnitine 0.2840 0.1943 0.5967 0.7129 0.1474 0.6697 0.5254 0.1667 0.3287 0.2419 0.0611 0.0543 0.0372 0.6769 0.8870 0.6051 0.4378 0.1880 0.2727 0.1293 icosadienoylcarnitine 0.5214 0.3012 0.8343 1.0329 0.1650 0.9940 0.5083 0.2399 0.3469 0.2398 0.0562 0.0677 0.0550 0.5345 0.6858 0.8564 0.5815 0.1181 0.2175 0.0475 Icosanoylcarnitine 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 icosatrienoylcarnitine 0.0944 0.0772 0.1276 0.2224 0.0228 0.1781 0.0848 0.0492 0.0472 0.0417 0.0105 0.0105 0.0066 0.0696 0.0947 0.1484 0.0934 0.0186 0.0279 0.0092 icoseneoylcarnitine 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 isovalerylcarnitine 1.1371 0.9347 1.4548 2.8231 0.9664 1.4876 2.0545 0.3015 1.9744 0.3616 0.7420 0.9882 0.7821 1.5630 1.2236 1.3660 1.5534 0.3730 0.5316 0.1358 lauroylcarnitine 0.4107 0.2401 0.7317 0.4193 0.0576 1.0009 0.5135 0.3418 0.2975 0.5073 0.0871 0.0651 0.0654 0.7683 1.0217 0.6840 0.6331 0.2549 0.3293 0.2037 L-Carnitine 299.6038 125.5179 385.8503 777.3591 107.7137 441.4766 442.8754 80.3070 698.7451 55.0813 687.1565 734.0856 495.2323 484.0477 437.8671 387.2361 353.1963 78.6520 195.0307 53.6860 Linoelaidyl carnitine 2.1025 1.3334 2.5223 2.5717 0.7035 2.6919 1.8427 1.6663 1.6570 1.9490 0.6404 0.6551 0.4212 1.9070 3.7611 2.9335 3.0581 1.0218 1.8559 0.8984 myristoylcarnitine 1.0495 0.7300 1.3978 1.1850 0.2725 1.9070 1.1672 1.0760 0.9343 1.1827 0.3413 0.3627 0.3649 1.5504 2.1358 1.3872 1.4124 0.6049 0.9604 0.7191 octanoylcarnitine 0.2112 0.1088 0.4179 0.3031 0.0618 0.5203 0.2297 0.1366 0.2257 0.1533 0.0598 0.0398 0.0259 0.4676 0.7356 0.4736 0.2172 0.1163 0.1506 0.0695 Octenoylcarnitine 0.0255 0.0390 0.0597 0.0303 0.0031 0.0439 0.0303 0.0082 0.0113 0.0084 0.0033 0.0032 0.0041 0.0242 0.0869 0.0445 0.0355 0.0059 0.0103 0.0056 Oleoylcarnitine 2.7247 1.7331 3.1415 3.4424 1.2356 4.1077 2.7438 2.3134 2.8587 3.0086 0.9557 1.1285 1.0040 3.6295 4.4440 3.2500 3.7377 1.5416 2.5356 1.2719 Palmitoleylcarnitine 1.9973 0.9657 2.3187 1.7240 0.3673 3.2745 2.0186 1.8254 1.7849 2.2015 0.3837 0.3728 0.3608 2.6316 3.0896 2.3783 2.5067 0.9053 1.5741 0.9288 palmitoylcarnitine 2.6032 2.0009 2.9087 3.1859 1.2036 4.0392 2.9591 2.3876 2.6473 2.7571 1.1606 1.3258 1.2453 3.4065 4.6390 3.2950 3.8190 1.5069 2.7191 1.5694 propionylcarnitine 1.9865 3.9293 2.3617 3.0066 4.6204 2.9391 1.5886 2.7709 2.0578 2.7120 2.8174 3.9937 4.0633 2.2433 9.9879 9.4323 12.7799 4.0108 8.6229 2.6676 Stearoylcarnitine 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 Tetradecadiencarnitine 0.3841 0.1217 0.7502 0.3469 0.0287 0.7730 0.3641 0.1866 0.1058 0.2308 0.0264 0.0212 0.0190 0.3341 0.7790 0.6055 0.5682 0.1417 0.2193 0.1177 Tetradecenoylcarnitine 1.1395 0.4289 1.6890 0.9608 0.1552 2.2763 1.2573 0.9177 0.6881 1.1458 0.1192 0.1352 0.1225 1.6132 1.8721 1.3564 1.4398 0.6288 0.7097 0.5458 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type 3-hydroxylauroylcarnitine 71464535 C12-OH UM_Target_ID 3-Hydroxylinoleoylcarnitine 78076335 C18:2-OH UM_Target_ID 3-hydroxymyristoylcarnitine 71464541 C14-OH UM_Target_ID 3-Hydroxypalmitoylcarnitine 71464553 C16-OH UM_Target_ID Acetylcarnitine 1 C2 UM_Target_ID arachidonoylcarnitine 71464486 C20:4 UM_Target_ID butyrylcarnitine 439829 C4 UM_Target_ID Decanoylcarnitine 3332882 C10:0 UM_Target_ID decenoylcarnitine 54074509 C10:1 UM_Target_ID Dodecenoylcarnitine 53481671 C12:1 UM_Target_ID Glutarylcarnitine 71317118 C5:0-DC UM_Target_ID Hexanoylcarnitine 6426853 C6:0 UM_Target_ID icosadienoylcarnitine 163425699 C20:2 UM_Target_ID Icosanoylcarnitine 53477833 C20 UM_Target_ID icosatrienoylcarnitine C20:3 UM_Target_ID icoseneoylcarnitine 71464507 C20:1 UM_Target_ID isovalerylcarnitine 6426852 C5:0 UM_Target_ID lauroylcarnitine 168381 C12:0 UM_Target_ID L-Carnitine 10917 L-Carnitine UM_Target_ID Linoelaidyl carnitine 53477834 C18:2 UM_Target_ID myristoylcarnitine 3413344 C14:0 UM_Target_ID octanoylcarnitine 123701 C8:0 UM_Target_ID Octenoylcarnitine 71464472 C8:1 UM_Target_ID Oleoylcarnitine 166952 C18:1 UM_Target_ID Palmitoleylcarnitine 76040365 C16:1 UM_Target_ID palmitoylcarnitine 461 C16:0 UM_Target_ID propionylcarnitine 107738 C3 UM_Target_ID Stearoylcarnitine 5282 C18:0 UM_Target_ID Tetradecadiencarnitine 53481681 C14:2 UM_Target_ID Tetradecenoylcarnitine 22833575 C14:1 UM_Target_ID METABOLITES_END #END