#METABOLOMICS WORKBENCH araskind_20150612_9797071_mwtab.txt DATATRACK_ID:320 STUDY_ID:ST000215 ANALYSIS_ID:AN000318 PROJECT_ID:PR000169 VERSION 1 CREATED_ON 01-15-2019 #PROJECT PR:PROJECT_TITLE Regulation of hepatic metabolism PR:PROJECT_SUMMARY Examine metabolite in liver and plasma before and after 13C-glucose load in wild PR:PROJECT_SUMMARY type, LIRKO and LIRFKO mice PR:INSTITUTE University of Chicago PR:DEPARTMENT Jesse Brown VA Medical Center PR:LABORATORY Unterman Lab PR:LAST_NAME Unterman PR:FIRST_NAME Terry PR:ADDRESS Chicago, IL PR:EMAIL unterman@uic.edu PR:PHONE 847-778-0943 PR:DOI http://dx.doi.org/10.21228/M8N88K #STUDY ST:STUDY_TITLE Liver and Plasma metaboites for 13C-glucose load in wild type, LIRKO GTT 1 mice ST:STUDY_TYPE 13C mass isotopomer analysis (flux studies) ST:STUDY_SUMMARY Mice were fasted for 18 hr overnight then sacrificed or treated with ST:STUDY_SUMMARY 13C-U-glucose (2 g/kg ip) and sacrificed 1 hr later by decapitation and liver ST:STUDY_SUMMARY was immediately freeze-clamped and stored in liquid N2 and then at -80 C. Wild ST:STUDY_SUMMARY type (IR and IR/FoxO1 floxed) mice were sacrificed after fasting and 1 hr ST:STUDY_SUMMARY post-glucose treatment. Liver-specific insulin receptor knockout (LIRKO) and ST:STUDY_SUMMARY insulin receptor/FoxO1 double knockout (LIRFKO) mice were sacrificed 1 hr post ST:STUDY_SUMMARY glucose treatment. ST:STUDY_SUMMARY http://www.nature.com/ncomms/2015/150512/ncomms8079/full/ncomms8079.html ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@umich.edu ST:PHONE - #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:SPECIES_GROUP Mammal #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0003002 S00013145 treatment:glucose | Strain:IRF floxed SUBJECT_SAMPLE_FACTORS SU0003001 S00013143 treatment:glucose | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003001 S00013142 treatment:glucose | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003001 S00013144 treatment:glucose | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003004 S00013152 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003004 S00013153 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003004 S00013154 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003004 S00013155 treatment:glucose | Strain:LIRFKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013146 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013147 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013148 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013149 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013150 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003003 S00013151 treatment:glucose | Strain:LIRKO SUBJECT_SAMPLE_FACTORS SU0003000 S00013136 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013137 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013138 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013139 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013140 treatment:none | Strain:IR floxed SUBJECT_SAMPLE_FACTORS SU0003000 S00013141 treatment:none | Strain:IR floxed #COLLECTION CO:SAMPLE_TYPE Liver CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_PROTOCOL_FILENAME Fluxomics_analysis_protocol-2015-03-11.docx SP:SAMPLEPREP_SUMMARY - #CHROMATOGRAPHY CH:METHODS_ID AQM011 CH:METHODS_FILENAME ALPHA_KETO_ACIDS-FULL.M.zip CH:INSTRUMENT_NAME Agilent 7890A CH:COLUMN_NAME Agilent DB5-MS (30m × 0.25mm, 0.25um) CH:CHROMATOGRAPHY_TYPE GC #ANALYSIS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ANALYSIS_TYPE MS AN:ACQUISITION_PARAMETERS_FILE ALPHA_KETO_ACIDS-FULL.M.zip AN:PROCESSING_PARAMETERS_FILE EX00258-flux-MassHunterQuant-GlyTCA-DataAnalysis-GCMS-Method.m.zip #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975C MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Area MS_METABOLITE_DATA_START Samples S00013145 S00013143 S00013142 S00013144 S00013152 S00013153 S00013154 S00013155 S00013146 S00013147 S00013148 S00013149 S00013150 S00013151 S00013136 S00013137 S00013138 S00013139 S00013140 S00013141 Factors treatment:glucose | Strain:IRF floxed treatment:glucose | Strain:IR floxed treatment:glucose | Strain:IR floxed treatment:glucose | Strain:IR floxed treatment:glucose | Strain:LIRFKO treatment:glucose | Strain:LIRFKO treatment:glucose | Strain:LIRFKO treatment:glucose | Strain:LIRFKO treatment:glucose | Strain:LIRKO treatment:glucose | Strain:LIRKO treatment:glucose | Strain:LIRKO treatment:glucose | Strain:LIRKO treatment:glucose | Strain:LIRKO treatment:glucose | Strain:LIRKO treatment:none | Strain:IR floxed treatment:none | Strain:IR floxed treatment:none | Strain:IR floxed treatment:none | Strain:IR floxed treatment:none | Strain:IR floxed treatment:none | Strain:IR floxed Alanine_123 738692.6962 1049154.1670 162015.6519 487804.8533 246267.6774 912939.4142 995231.9188 143037.0408 1065461.2880 103198.5807 1003759.7610 878056.7143 516554.3236 690009.8590 131470.9018 679014.5625 653616.1832 225280.7830 207228.4148 47712.3723 Alanine_123_13C_1 71263.1825 91463.4622 8156.5372 49949.7724 10344.4467 79757.5235 84582.6988 64574.9829 1491.2624 45863.6283 52911.1094 33382.0331 44777.8230 4020.9963 4079.4270 alpha-Ketoglutarate_12345 3249.4756 4880.9912 924.4083 2132.8394 746.3523 3079.3576 2646.5626 962.8433 2887.1189 1915.7523 1499.6501 1251.5252 3515.6352 1729.3565 3767.5492 4660.4378 3921.9261 1625.5479 975.7395 alpha-Ketoglutarate_12345_13C_1 353.3213 1365.2878 217.8550 329.0563 465.7832 31.6778 290.8566 130.5523 347.2514 326.7457 171.1481 570.2727 517.9660 141.0012 558.9242 743.5315 30.8629 97.9268 alpha-Ketoglutarate_12345_13C_2 401.7553 697.4664 111.1895 300.1828 532.8479 111.4410 223.8341 551.8321 469.2233 916.4384 527.6061 803.7987 78.9785 841.3840 1103.9186 614.8049 alpha-Ketoglutarate_12345_13C_3 175.1855 870.3260 286.9722 750.1332 0.7200 1521.9817 1121.0559 Fumarate_1234 465453.8935 255979.6275 97872.0879 96279.8263 103929.8068 254941.6312 227430.6710 127934.1074 276326.1915 29456.1712 349284.5672 278750.3701 266488.6067 244041.7254 40363.5932 198078.0369 254363.8990 163220.7407 126840.6560 89662.9662 Fumarate_1234_13C_1 31924.2322 22384.3168 3140.1692 11981.3033 4661.9763 19935.9911 18988.0828 5313.0296 19022.0570 4971.3469 31405.6714 28068.5640 22120.9149 20937.7198 1004.1897 5450.7560 5652.9723 2504.3841 1653.0153 1067.1847 Fumarate_1234_13C_2 15991.8801 11014.6848 16.4870 7228.7011 1319.2103 14630.7562 12330.9818 6627.5442 9366.3212 1791.6006 15739.6795 14747.9230 18157.3218 13616.5562 1441.1719 1869.6131 447.4402 1135.3373 Lactate_123 1771692.6880 2403925.1900 347809.5572 1338101.7190 677227.6944 1927718.0540 2504571.9880 315143.4646 2414676.6610 233011.5652 1576713.2430 1581721.6260 935658.4689 1604372.9550 260668.6247 1526362.3380 1432448.6600 647676.1112 443511.6200 98480.7725 Lactate_123_13C_1 175804.3936 189883.4391 15428.6782 125398.2879 46303.7364 167753.6845 212089.8617 22087.6434 156474.1705 15332.7270 92259.5761 119672.7906 66681.1011 102157.9755 1380.3096 2826.3057 3822.4505 2823.7982 2620.5216 1937.5461 Lactate_123_13C_2 173794.2161 190481.7589 11049.7570 104004.1028 50626.7426 160820.5535 227708.0911 22466.3064 156311.4400 17835.5545 104597.3864 121079.0243 67419.2692 121272.5820 400.8737 Lactate_123_13C_3 564841.2440 599729.8021 61539.0598 401358.0748 101109.2389 341226.8358 395751.8217 52500.5375 445068.7061 42729.6358 453210.2905 399679.2480 239731.5179 442431.0161 3.8973 17.6705 774.5743 Pyruvate_123 93194.7129 61296.5326 7249.8472 25801.0275 7203.6984 20168.4854 42538.3044 4215.5031 28337.1355 5090.0393 28733.5785 17999.6702 15587.5875 26167.4078 7194.1851 42045.8518 46428.8729 6838.0396 11419.8514 4426.0595 Pyruvate_123_13C_1 6736.5972 3907.7334 164.9495 2048.3495 636.3879 1099.0821 2094.9760 257.0530 964.9538 90.2369 1847.9164 1035.4983 924.7698 1054.8036 908.8380 1691.8899 1263.0000 155.2166 302.6235 263.5736 Pyruvate_123_13C_2 2788.8978 1827.2312 209.4778 933.1046 324.3978 1340.8471 1695.6569 895.1574 238.6749 983.6148 522.3573 647.7400 619.0911 152.3559 1044.1383 553.9851 78.8068 265.1861 415.9549 Pyruvate_123_13C_3 71.4462 354.4413 418.4735 245.9023 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant Alanine_123 5950 C00041 ALA_123 UM_Target_ID Alanine_123_13C_1 5950 C00041 ALA_123_13C_1 UM_Target_ID alpha-Ketoglutarate_12345 51 C00026 AKG_12345 UM_Target_ID alpha-Ketoglutarate_12345_13C_1 51 C00026 AKG_12345_13C_1 UM_Target_ID alpha-Ketoglutarate_12345_13C_2 51 C00026 AKG_12345_13C_2 UM_Target_ID alpha-Ketoglutarate_12345_13C_3 51 C00026 AKG_12345_13C_3 UM_Target_ID Fumarate_1234 444972 C00122 FUM_1234 UM_Target_ID Fumarate_1234_13C_1 444972 C00122 FUM_1234_13C_1 UM_Target_ID Fumarate_1234_13C_2 444972 C00122 FUM_1234_13C_2 UM_Target_ID Lactate_123 107689 C00186 LAC_123 UM_Target_ID Lactate_123_13C_1 107689 C00186 LAC_123_13C_1 UM_Target_ID Lactate_123_13C_2 107689 C00186 LAC_123_13C_2 UM_Target_ID Lactate_123_13C_3 107689 C00186 LAC_123_13C_3 UM_Target_ID Pyruvate_123 1060 C00022 PYR_123 UM_Target_ID Pyruvate_123_13C_1 1060 C00022 PYR_123_13C_1 UM_Target_ID Pyruvate_123_13C_2 1060 C00022 PYR_123_13C_2 UM_Target_ID Pyruvate_123_13C_3 1060 C00022 PYR_123_13C_3 UM_Target_ID METABOLITES_END #END