#METABOLOMICS WORKBENCH rgn5011_20150729_9291911_mwtab.txt DATATRACK_ID:345 STUDY_ID:ST000233 ANALYSIS_ID:AN000349 PROJECT_ID:PR000120 VERSION 1 CREATED_ON 2016-09-17 #PROJECT PR:PROJECT_TITLE Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts PR:PROJECT_TITLE Microbiota-Host Metabolic Homeostasis in Mice PR:PROJECT_SUMMARY - PR:INSTITUTE Pennsylvania State University PR:DEPARTMENT Molecular Toxicology PR:LAST_NAME Andrew PR:FIRST_NAME Patterson PR:ADDRESS - PR:EMAIL adp117@PSU.EDU, RGN5011@psu.edu PR:PHONE - #STUDY ST:STUDY_TITLE Metabolomics Reveals that Aryl Hydrocarbon Receptor Activation by Environmental ST:STUDY_TITLE Induces Systemic Metabolic Dysfunction in Mice (Part II) ST:STUDY_TYPE Time course ST:STUDY_SUMMARY 12 C57BL/6J male mice were acclimatized for 1 week and then divided into two ST:STUDY_SUMMARY and trained to eat dough pills. One group was control and the other was exposed ST:STUDY_SUMMARY TCDF through the dough pills. The mice were treated for 5 days at a dosage ST:STUDY_SUMMARY of TCDF for a final concentration of 5μg/kg body weight. Also looked at were ST:STUDY_SUMMARY mice and they were treated the same way as the C57BL/6J mice. ST:INSTITUTE Pennsylvania State University ST:DEPARTMENT Department of Veterinary and Biomedical Sciences ST:LAST_NAME Patterson ST:FIRST_NAME Andrew ST:ADDRESS 101 Life Sciences, University Park, State college, 16802 ST:EMAIL rgn5011@psu.edu ST:PHONE - #SUBJECT SU:SUBJECT_TYPE Mouse SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN C57BL/6J #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS Mouse 101 Strain:AHR -/- | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 102 Strain:AHR -/- | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 103 Strain:AHR -/- | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 104 Strain:AHR -/- | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 105 Strain:AHR -/- | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 201 Strain:AHR -/- | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 202 Strain:AHR -/- | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 203 Strain:AHR -/- | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 204 Strain:AHR -/- | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 205 Strain:AHR -/- | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 301 Strain:WT | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 302 Strain:WT | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 303 Strain:WT | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 304 Strain:WT | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 305 Strain:WT | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 401 Strain:WT | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 402 Strain:WT | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 403 Strain:WT | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 405 Strain:WT | Treatment:TCDF Treatment #COLLECTION CO:COLLECTION_SUMMARY Feces and urine were collected every other day. Then Liver, blood and CO:COLLECTION_SUMMARY tissue were collected after death CO:SAMPLE_TYPE Feces, Urine, Liver, Blood, Intestinal tissue #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME GC_Sample_Prep.docx SP:PROCESSING_METHOD Homogenization SP:EXTRACTION_METHOD Methanol-Chloroform SP:EXTRACT_ENRICHMENT centerfugation SP:EXTRACT_CLEANUP bottom layer was dried with N2 gas, resuspended in MEOH/HCL and then hexane and SP:EXTRACT_CLEANUP top layer was collected SP:SAMPLE_RESUSPENSION resuspended in hexane SP:SAMPLE_SPIKING C15:0 free acid and methy ester of C17:0 at 5 ?L SP:ORGAN Liver #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 5975C MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:MS_COMMENTS Serum Fatty Acids #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak area MS_METABOLITE_DATA_START Samples 101 102 103 104 105 201 202 203 204 205 301 302 303 304 305 401 402 403 405 Factors Strain:AHR -/- | Treatment:Control Strain:AHR -/- | Treatment:Control Strain:AHR -/- | Treatment:Control Strain:AHR -/- | Treatment:Control Strain:AHR -/- | Treatment:Control Strain:AHR -/- | Treatment:TCDF Treatment Strain:AHR -/- | Treatment:TCDF Treatment Strain:AHR -/- | Treatment:TCDF Treatment Strain:AHR -/- | Treatment:TCDF Treatment Strain:AHR -/- | Treatment:TCDF Treatment Strain:WT | Treatment:Control Strain:WT | Treatment:Control Strain:WT | Treatment:Control Strain:WT | Treatment:Control Strain:WT | Treatment:Control Strain:WT | Treatment:TCDF Treatment Strain:WT | Treatment:TCDF Treatment Strain:WT | Treatment:TCDF Treatment Strain:WT | Treatment:TCDF Treatment FA(15:0) 5404542.0000 5156123.0000 4329846.0000 4356055.0000 5014871.0000 4807747.0000 4112529.0000 4959657.0000 4389321.0000 4762383.0000 5350962.0000 4361507.0000 4831032.0000 4030006.0000 4422929.0000 4421577.0000 4064833.0000 4831747.0000 4341435.0000 FA(16:0) 6101449.0000 6463901.0000 5102105.0000 5849114.0000 6535835.0000 6519217.0000 5801504.0000 6397721.0000 5565339.0000 6839948.0000 8136341.0000 7043406.0000 6270519.0000 4990965.0000 5115495.0000 3509033.0000 4790589.0000 3881753.0000 4498282.0000 FA(17:0) 7073551.0000 6211858.0000 5926779.0000 5856807.0000 6055870.0000 6500216.0000 5532941.0000 6626032.0000 5924246.0000 6445645.0000 6899472.0000 5705902.0000 6227521.0000 5571923.0000 5696263.0000 6556465.0000 5856927.0000 5620608.0000 5887417.0000 FA(18:0) 2963364.0000 2994257.0000 2668343.0000 3155257.0000 3292207.0000 3379363.0000 2893962.0000 3374797.0000 2755182.0000 3558503.0000 3661054.0000 2866470.0000 2932827.0000 2207039.0000 2355526.0000 1439391.0000 2335521.0000 1915792.0000 2300227.0000 FA(18:2) 1251421.0000 2278777.0000 1320130.0000 1695554.0000 1940435.0000 1409000.0000 1763262.0000 1910017.0000 1267170.0000 2047997.0000 1565785.0000 999797.0000 867363.0000 1218399.0000 1358180.0000 2548842.0000 1700809.0000 1510610.0000 1834780.0000 FA(22:0) 190370.0000 205183.0000 116507.0000 133909.0000 150056.0000 166007.0000 162380.0000 165204.0000 155167.0000 150025.0000 204400.0000 144413.0000 163653.0000 96311.0000 134176.0000 92336.0000 76946.0000 74671.0000 84942.0000 FA(22:6) 1337141.0000 1644756.0000 1527616.0000 1282108.0000 1489507.0000 1379816.0000 1416968.0000 1654191.0000 1308178.0000 1857871.0000 2076016.0000 1725068.0000 697723.0000 792214.0000 1241721.0000 2141948.0000 1622036.0000 1260191.0000 1412701.0000 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type FA(15:0) Penn_State_Univ_ID FA(16:0) Penn_State_Univ_ID FA(17:0) Penn_State_Univ_ID FA(18:0) Penn_State_Univ_ID FA(18:2) Penn_State_Univ_ID FA(22:0) Penn_State_Univ_ID FA(22:6) Penn_State_Univ_ID METABOLITES_END #END