#METABOLOMICS WORKBENCH rgn5011_20150729_9291911_mwtab.txt DATATRACK_ID:345 STUDY_ID:ST000233 ANALYSIS_ID:AN000350 PROJECT_ID:PR000120 VERSION 1 CREATED_ON 2016-09-17 #PROJECT PR:PROJECT_TITLE Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts PR:PROJECT_TITLE Microbiota-Host Metabolic Homeostasis in Mice PR:PROJECT_SUMMARY - PR:INSTITUTE Pennsylvania State University PR:DEPARTMENT Molecular Toxicology PR:LAST_NAME Andrew PR:FIRST_NAME Patterson PR:ADDRESS - PR:EMAIL adp117@PSU.EDU, RGN5011@psu.edu PR:PHONE - #STUDY ST:STUDY_TITLE Metabolomics Reveals that Aryl Hydrocarbon Receptor Activation by Environmental ST:STUDY_TITLE Induces Systemic Metabolic Dysfunction in Mice (Part II) ST:STUDY_TYPE Time course ST:STUDY_SUMMARY 12 C57BL/6J male mice were acclimatized for 1 week and then divided into two ST:STUDY_SUMMARY and trained to eat dough pills. One group was control and the other was exposed ST:STUDY_SUMMARY TCDF through the dough pills. The mice were treated for 5 days at a dosage ST:STUDY_SUMMARY of TCDF for a final concentration of 5μg/kg body weight. Also looked at were ST:STUDY_SUMMARY mice and they were treated the same way as the C57BL/6J mice. ST:INSTITUTE Pennsylvania State University ST:DEPARTMENT Department of Veterinary and Biomedical Sciences ST:LAST_NAME Patterson ST:FIRST_NAME Andrew ST:ADDRESS 101 Life Sciences, University Park, State college, 16802 ST:EMAIL rgn5011@psu.edu ST:PHONE - #SUBJECT SU:SUBJECT_TYPE Mouse SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN C57BL/6J #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS Mouse 101 Strain:AHR -/- | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 102 Strain:AHR -/- | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 103 Strain:AHR -/- | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 104 Strain:AHR -/- | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 105 Strain:AHR -/- | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 201 Strain:AHR -/- | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 202 Strain:AHR -/- | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 203 Strain:AHR -/- | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 204 Strain:AHR -/- | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 205 Strain:AHR -/- | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 301 Strain:WT | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 302 Strain:WT | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 303 Strain:WT | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 304 Strain:WT | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 305 Strain:WT | Treatment:Control SUBJECT_SAMPLE_FACTORS Mouse 401 Strain:WT | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 402 Strain:WT | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 403 Strain:WT | Treatment:TCDF Treatment SUBJECT_SAMPLE_FACTORS Mouse 405 Strain:WT | Treatment:TCDF Treatment #COLLECTION CO:COLLECTION_SUMMARY Feces and urine were collected every other day. Then Liver, blood and CO:COLLECTION_SUMMARY tissue were collected after death CO:SAMPLE_TYPE Feces, Urine, Liver, Blood, Intestinal tissue #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME GC_Sample_Prep.docx SP:PROCESSING_METHOD Homogenization SP:EXTRACTION_METHOD Methanol-Chloroform SP:EXTRACT_ENRICHMENT centerfugation SP:EXTRACT_CLEANUP bottom layer was dried with N2 gas, resuspended in MEOH/HCL and then hexane and SP:EXTRACT_CLEANUP top layer was collected SP:SAMPLE_RESUSPENSION resuspended in hexane SP:SAMPLE_SPIKING C15:0 free acid and methy ester of C17:0 at 5 ?L SP:ORGAN Liver #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 5975C MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:MS_COMMENTS Liver #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak area MS_METABOLITE_DATA_START Samples 101 102 103 104 105 201 202 203 204 205 301 302 303 304 305 401 402 403 405 Factors Strain:AHR -/- | Treatment:Control Strain:AHR -/- | Treatment:Control Strain:AHR -/- | Treatment:Control Strain:AHR -/- | Treatment:Control Strain:AHR -/- | Treatment:Control Strain:AHR -/- | Treatment:TCDF Treatment Strain:AHR -/- | Treatment:TCDF Treatment Strain:AHR -/- | Treatment:TCDF Treatment Strain:AHR -/- | Treatment:TCDF Treatment Strain:AHR -/- | Treatment:TCDF Treatment Strain:WT | Treatment:Control Strain:WT | Treatment:Control Strain:WT | Treatment:Control Strain:WT | Treatment:Control Strain:WT | Treatment:Control Strain:WT | Treatment:TCDF Treatment Strain:WT | Treatment:TCDF Treatment Strain:WT | Treatment:TCDF Treatment Strain:WT | Treatment:TCDF Treatment FA(15:0) 761097.0000 819380.0000 800474.0000 762684.0000 902919.0000 670639.0000 760924.0000 639671.0000 872323.0000 819337.0000 943998.0000 902510.0000 869183.0000 839426.0000 925971.0000 697202.0000 811410.0000 857490.0000 844572.0000 FA(16:0) 11510080.0000 11502077.0000 14975285.0000 12049414.0000 14904496.0000 10498868.0000 12582869.0000 9743790.0000 16712954.0000 13418382.0000 18503292.0000 17336783.0000 16590918.0000 13877611.0000 17132619.0000 12080169.0000 13700083.0000 12739530.0000 13560441.0000 FA(16:1n7) 342581.0000 410249.0000 567713.0000 585340.0000 637805.0000 412247.0000 450660.0000 415856.0000 336593.0000 503398.0000 625901.0000 661413.0000 436137.0000 518721.0000 617309.0000 642451.0000 660295.0000 751670.0000 753131.0000 FA(17:0) 1085162.0000 1144718.0000 1179332.0000 1063466.0000 1267725.0000 933911.0000 966602.0000 905886.0000 1438081.0000 1184558.0000 1294550.0000 1310942.0000 1243921.0000 1200209.0000 1344049.0000 972474.0000 1259578.0000 1344551.0000 1234472.0000 FA(18:0) 8248608.0000 8100962.0000 10924783.0000 8475302.0000 10539775.0000 7005080.0000 7977703.0000 7295390.0000 12046678.0000 10114663.0000 12150848.0000 12585656.0000 8967661.0000 9019924.0000 11562418.0000 9185392.0000 9309233.0000 1801934.0000 1496003.0000 FA(18:1n9) 4605129.0000 5252169.0000 6784835.0000 5774464.0000 6839017.0000 4819726.0000 5159472.0000 5163968.0000 6334061.0000 5841391.0000 7346353.0000 8221927.0000 5269338.0000 6310441.0000 7823297.0000 9774463.0000 11310760.0000 14622791.0000 12972374.0000 FA(18:2n6) 8053683.0000 8319391.0000 9802719.0000 7729360.0000 10277324.0000 7028427.0000 8038583.0000 8035529.0000 8502032.0000 9086460.0000 9204410.0000 12869587.0000 8711897.0000 11010197.0000 8586695.0000 13741946.0000 13672410.0000 16131593.0000 14432630.0000 FA(18:3n3) 35925.0000 49255.0000 53062.0000 46539.0000 88769.0000 56825.0000 52039.0000 54421.0000 46375.0000 62296.0000 56486.0000 64967.0000 64289.0000 67215.0000 59217.0000 66417.0000 78389.0000 69501.0000 67337.0000 FA(20:0) 138335.0000 94323.0000 213514.0000 126784.0000 184183.0000 75995.0000 88906.0000 53316.0000 131753.0000 74100.0000 128286.0000 216980.0000 188041.0000 260302.0000 271376.0000 129067.0000 123281.0000 158583.0000 101343.0000 FA(20:1n9) 171813.0000 156469.0000 200071.0000 181345.0000 269509.0000 156113.0000 188719.0000 145130.0000 224737.0000 230645.0000 331846.0000 399967.0000 158844.0000 106757.0000 220576.0000 419348.0000 446907.0000 475110.0000 449448.0000 FA(20:2n6) 105051.0000 150446.0000 241908.0000 141306.0000 262540.0000 161791.0000 139316.0000 134270.0000 103463.0000 163871.0000 167934.0000 320430.0000 157316.0000 153487.0000 117084.0000 369082.0000 310613.0000 337655.0000 276681.0000 FA(20:3n3) 627617.0000 740482.0000 1101218.0000 918501.0000 1029707.0000 732989.0000 784783.0000 788529.0000 818049.0000 821380.0000 814414.0000 1025034.0000 917107.0000 582639.0000 776736.0000 1045414.0000 1463643.0000 1557639.0000 1483019.0000 FA(20:3n6) 158945.0000 124926.0000 206994.0000 201935.0000 134072.0000 123198.0000 80477.0000 379571.0000 142017.0000 29624.0000 35589.0000 144832.0000 153487.0000 268518.0000 619791.0000 801012.0000 1161412.0000 1106860.0000 FA(20:4n6) 6930624.0000 7358805.0000 10362682.0000 8260199.0000 9850542.0000 6787322.0000 8370782.0000 6870600.0000 8136638.0000 9548958.0000 6623284.0000 9856716.0000 7346146.0000 7677594.0000 8798286.0000 11045414.0000 12208159.0000 12852115.0000 8933879.0000 FA(20:5n3) 859093.0000 697912.0000 973903.0000 742244.0000 943626.0000 726010.0000 603121.0000 666858.0000 586615.0000 686991.0000 1031130.0000 1653367.0000 1098280.0000 923319.0000 1442525.0000 5450811.0000 4363799.0000 9532082.0000 4864200.0000 FA(22:0) 475360.0000 415987.0000 545873.0000 419452.0000 605565.0000 407114.0000 519323.0000 438085.0000 688620.0000 543072.0000 779304.0000 683756.0000 456921.0000 439598.0000 663498.0000 311421.0000 403574.0000 420083.0000 464878.0000 FA(22:1n9) 352633.0000 328282.0000 452868.0000 323682.0000 482635.0000 474000.0000 416341.0000 397340.0000 908159.0000 400608.0000 961060.0000 654955.0000 457155.0000 458158.0000 587700.0000 956336.0000 846466.0000 936832.0000 907813.0000 FA(22:6n3) 6608093.0000 6577133.0000 8880824.0000 6812036.0000 9358620.0000 6725946.0000 7744065.0000 7365180.0000 7110007.0000 8416503.0000 7474819.0000 9503413.0000 7515195.0000 8419772.0000 8935749.0000 15615892.0000 10040687.0000 10152992.0000 9973097.0000 FA(24:0) 314006.0000 296025.0000 391406.0000 304242.0000 439745.0000 305656.0000 342164.0000 278636.0000 484559.0000 349110.0000 474342.0000 472627.0000 304053.0000 287099.0000 449924.0000 256061.0000 344939.0000 315676.0000 387648.0000 FA(24:1n9) 268314.0000 272855.0000 483553.0000 350572.0000 500653.0000 301991.0000 266278.0000 279492.0000 484824.0000 353437.0000 427543.0000 442615.0000 312504.0000 341663.0000 376809.0000 467636.0000 513073.0000 509392.0000 520536.0000 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type FA(15:0) Penn_State_Univ_ID FA(16:0) Penn_State_Univ_ID FA(16:1n7) Penn_State_Univ_ID FA(17:0) Penn_State_Univ_ID FA(18:0) Penn_State_Univ_ID FA(18:1n9) Penn_State_Univ_ID FA(18:2n6) Penn_State_Univ_ID FA(18:3n3) Penn_State_Univ_ID FA(20:0) Penn_State_Univ_ID FA(20:1n9) Penn_State_Univ_ID FA(20:2n6) Penn_State_Univ_ID FA(20:3n3) Penn_State_Univ_ID FA(20:3n6) Penn_State_Univ_ID FA(20:4n6) Penn_State_Univ_ID FA(20:5n3) Penn_State_Univ_ID FA(22:0) Penn_State_Univ_ID FA(22:1n9) Penn_State_Univ_ID FA(22:6n3) Penn_State_Univ_ID FA(24:0) Penn_State_Univ_ID FA(24:1n9) Penn_State_Univ_ID METABOLITES_END #END