#METABOLOMICS WORKBENCH araskind_20150612_9929241_mwtab.txt DATATRACK_ID:315 STUDY_ID:ST000272 ANALYSIS_ID:AN000435 PROJECT_ID:PR000216
VERSION             	1
CREATED_ON          	2016-09-17
#PROJECT
PR:PROJECT_TITLE                 	Adipose Oxidation
PR:PROJECT_SUMMARY               	Role of Fatty Acid Oxidation in Adipose Tissue
PR:INSTITUTE                     	Johns Hopkins University
PR:DEPARTMENT                    	Biological Chemistry
PR:LABORATORY                    	Wolfgang Lab
PR:LAST_NAME                     	Wolfgang
PR:FIRST_NAME                    	Michael
PR:ADDRESS                       	733 North Broadway, Suite G49  Baltimore, MD 21205-2196
PR:EMAIL                         	mwolfga1@jhmi.edu
PR:PHONE                         	443-287-7680
#STUDY
ST:STUDY_TITLE                   	Acyl-carnitine analysis (plasma)
ST:STUDY_TYPE                    	Adipose Acyl-carnitines
ST:STUDY_SUMMARY                 	To understand the contribution of adipose tissue fatty acid oxidation to
ST:STUDY_SUMMARY                 	metabolism, we generated mice with an adipose-specific knockout of carnitine
ST:STUDY_SUMMARY                 	2 (CPT2A?/?), an obligate step in mitochondrial long-chain fatty acid
ST:STUDY_SUMMARY                 	CPT2A?/? mice became hypothermic after an acute cold challenge, and CPT2A?/?
ST:STUDY_SUMMARY                 	adipose tissue (BAT) failed to upregulate thermogenic genes in response to
ST:STUDY_SUMMARY                 	stimulation. The adipose-specific loss of CPT2 resulted in diet-dependent
ST:STUDY_SUMMARY                 	in adiposity but did not result in changes in body weight on low- or high-fat
ST:STUDY_SUMMARY                 	Additionally, CPT2A?/? mice had suppressed high-fat diet-induced oxidative
ST:STUDY_SUMMARY                 	and inflammation in visceral white adipose tissue (WAT); however, high-fat
ST:STUDY_SUMMARY                 	glucose intolerance was not improved. These data show that fatty acid oxidation
ST:STUDY_SUMMARY                 	required for cold-induced thermogenesis in BAT and high-fat diet-induced
ST:STUDY_SUMMARY                 	stress and inflammation in WAT.
ST:INSTITUTE                     	University of Michigan
ST:DEPARTMENT                    	Biological Chemistry(Johns Hopkins University School of Medicine)
ST:LABORATORY                    	Wolfgang Lab(Johns Hopkins University School of Medicine)
ST:LAST_NAME                     	Wolfgang
ST:FIRST_NAME                    	Michael
ST:ADDRESS                       	733 North Broadway, Suite G49  Baltimore, MD 21205-2196
ST:EMAIL                         	mwolfga1@jhmi.edu
ST:PHONE                         	443-287-7680
ST:NUM_GROUPS                    	2
ST:TOTAL_SUBJECTS                	16
ST:STUDY_COMMENTS                	(CPT2A?/?) stands for Carnitine palmitoyltransferase 2 knock out
#SUBJECT
SU:SUBJECT_TYPE                  	Animal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENOTYPE_STRAIN               	Wild Type and CPT2A-/-.
SU:SPECIES_GROUP                 	Mammal
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	SU0002970	S00012834	Genotype:(CPT2A?/?)	
SUBJECT_SAMPLE_FACTORS           	SU0002970	S00012835	Genotype:(CPT2A?/?)	
SUBJECT_SAMPLE_FACTORS           	SU0002970	S00012836	Genotype:(CPT2A?/?)	
SUBJECT_SAMPLE_FACTORS           	SU0002970	S00012837	Genotype:(CPT2A?/?)	
SUBJECT_SAMPLE_FACTORS           	SU0002970	S00012838	Genotype:(CPT2A?/?)	
SUBJECT_SAMPLE_FACTORS           	SU0002970	S00012839	Genotype:(CPT2A?/?)	
SUBJECT_SAMPLE_FACTORS           	SU0002970	S00012840	Genotype:(CPT2A?/?)	
SUBJECT_SAMPLE_FACTORS           	SU0002970	S00012841	Genotype:(CPT2A?/?)	
SUBJECT_SAMPLE_FACTORS           	SU0002969	S00012826	Genotype:Wild type	
SUBJECT_SAMPLE_FACTORS           	SU0002969	S00012827	Genotype:Wild type	
SUBJECT_SAMPLE_FACTORS           	SU0002969	S00012828	Genotype:Wild type	
SUBJECT_SAMPLE_FACTORS           	SU0002969	S00012829	Genotype:Wild type	
SUBJECT_SAMPLE_FACTORS           	SU0002969	S00012830	Genotype:Wild type	
SUBJECT_SAMPLE_FACTORS           	SU0002969	S00012831	Genotype:Wild type	
SUBJECT_SAMPLE_FACTORS           	SU0002969	S00012832	Genotype:Wild type	
SUBJECT_SAMPLE_FACTORS           	SU0002969	S00012833	Genotype:Wild type	
#COLLECTION
CO:COLLECTION_SUMMARY            	-
CO:SAMPLE_TYPE                   	Tissues
#TREATMENT
TR:TREATMENT_SUMMARY             	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	-
SP:SAMPLEPREP_PROTOCOL_FILENAME  	Acyl-carnitines_analysis_protocol-CR-MK-20150528.docx
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1200
CH:COLUMN_NAME                   	Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
CH:METHODS_ID                    	AQM050
CH:METHODS_FILENAME              	QM-004-Xbridg2mm_ACar+_MRM-Insert.m
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	MRC2 (University of Michigan)
AN:ACQUISITION_PARAMETERS_FILE   	QM-004-Xbridg2mm_ACar+_MRM-Insert.m
AN:PROCESSING_PARAMETERS_FILE    	EX00250-acyl-carnitines-quant.m
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Agilent 6490 QQQ
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	pmol/mg sample
MS_METABOLITE_DATA_START
Samples	S00012834	S00012835	S00012836	S00012837	S00012838	S00012839	S00012840	S00012841	S00012826	S00012827	S00012828	S00012829	S00012830	S00012831	S00012832	S00012833
Factors	Genotype:(CPT2A?/?)	Genotype:(CPT2A?/?)	Genotype:(CPT2A?/?)	Genotype:(CPT2A?/?)	Genotype:(CPT2A?/?)	Genotype:(CPT2A?/?)	Genotype:(CPT2A?/?)	Genotype:(CPT2A?/?)	Genotype:Wild type	Genotype:Wild type	Genotype:Wild type	Genotype:Wild type	Genotype:Wild type	Genotype:Wild type	Genotype:Wild type	Genotype:Wild type
3-hydroxylauroylcarnitine	0.0013	0.0010	0.0009	0.0038	0.0023	0.0007	0.0053	0.0031	0.0052	0.0040	0.0042	0.0031	0.0018	0.0024	0.0028	0.0141
3-Hydroxylinoleoylcarnitine	0.0086	0.0103	0.0074	0.0328	0.0229	0.0050	0.0163	0.0151	0.0332	0.0144	0.0326	0.0259	0.0171	0.0119	0.0253	0.1227
3-hydroxymyristoylcarnitine	0.0034	0.0036	0.0032	0.0081	0.0056	0.0028	0.0093	0.0065	0.0109	0.0083	0.0096	0.0065	0.0041	0.0057	0.0054	0.0338
3-Hydroxypalmitoylcarnitine	0.0080	0.0107	0.0074	0.0207	0.0183	0.0057	0.0128	0.0117	0.0380	0.0180	0.0235	0.0231	0.0111	0.0111	0.0197	0.0777
Acetylcarnitine	2.0901	2.3903	2.2280	5.0642	3.6235	1.6427	1.8013	1.7754	9.3081	3.5338	4.9432	3.5403	2.8583	1.9466	3.8334	11.5698
arachidonoylcarnitine	0.0154	0.0157	0.0216	0.0356	0.0297	0.0120	0.0090	0.0151	0.0899	0.0279	0.0318	0.0385	0.0185	0.0127	0.0357	0.0746
butyrylcarnitine	0.1382	0.1897	0.1534	0.3703	0.1791	0.1482	0.0761	0.1942	0.3460	0.2161	0.4315	0.3168	0.1481	0.2256	0.2473	0.6871
Decanoylcarnitine	0.0124	0.0105	0.0062	0.0323	0.0131	0.0060	0.0023	0.0067	0.0202	0.0090	0.0142	0.0140	0.0124	0.0073	0.0208	0.0951
decenoylcarnitine	0.0018	0.0029	0.0020	0.0083	0.0028	0.0012	0.0050	0.0033	0.0050	0.0042	0.0048	0.0063	0.0032	0.0026	0.0052	0.0170
Dodecenoylcarnitine	0.0179	0.0077	0.0081	0.0219	0.0122	0.0129	0.0034	0.0060	0.0168	0.0069	0.0093	0.0088	0.0106	0.0053	0.0165	0.0734
Glutarylcarnitine	0.0012	0.0008	0.0005	0.0028	0.0017	0.0005	0.0008	0.0008	0.0077	0.0017	0.0022	0.0008	0.0010	0.0007	0.0016	0.0060
Hexanoylcarnitine	0.0155	0.0255	0.0146	0.0692	0.0447	0.0127	0.0191	0.0253	0.0736	0.0351	0.0563	0.0473	0.0282	0.0340	0.0434	0.1825
icosadienoylcarnitine	0.0144	0.0171	0.0157	0.0358	0.0249	0.0079	0.0037	0.0082	0.0986	0.0196	0.0336	0.0322	0.0154	0.0085	0.0286	0.1532
Icosanoylcarnitine	0.0000		0.0001	0.0002	0.0001	0.0006	0.0000	0.0005						0.0000	0.0004	0.0003
icosatrienoylcarnitine	0.0037	0.0050	0.0053	0.0090	0.0066	0.0027	0.0012	0.0030	0.0191	0.0056	0.0063	0.0088	0.0049	0.0026	0.0083	0.0221
icoseneoylcarnitine																
isovalerylcarnitine	0.0681	0.0592	0.0608	0.1297	0.0841	0.0509	0.0513	0.0960	0.1877	0.1242	0.1429	0.1028	0.0789	0.0663	0.1354	0.1665
lauroylcarnitine	0.0632	0.0245	0.0338	0.0812	0.0478	0.0431	0.0124	0.0233	0.0839	0.0340	0.0437	0.0331	0.0423	0.0256	0.0551	0.2383
L-Carnitine	12.5775	11.0841	9.2492	22.4435	18.8182	8.4666	13.4303	10.6708	50.4719	20.6230	33.7417	17.3530	12.1600	10.6873	19.8427	19.5840
Linoelaidyl carnitine	0.5226	0.4142	0.5453	0.7466	0.5896	0.4073	0.0944	0.2071	1.0714	0.3907	0.4097	0.3545	0.5499	0.1984	0.7209	1.1293
myristoylcarnitine	0.4486	0.2873	0.3803	0.6415	0.5055	0.4592	0.1012	0.1802	0.6841	0.2354	0.3036	0.2100	0.5178	0.1737	0.5696	1.0437
octanoylcarnitine	0.0062	0.0077	0.0049	0.0303	0.0149	0.0039	0.0031	0.0076	0.0200	0.0082	0.0144	0.0135	0.0127	0.0070	0.0178	0.0689
Octenoylcarnitine	0.0015	0.0017	0.0014	0.0056	0.0024	0.0014	0.0030	0.0045	0.0089	0.0035	0.0060	0.0039	0.0021	0.0033	0.0034	0.0099
Oleoylcarnitine	1.1995	1.1464	1.3970	1.5941	1.4630	1.3074	0.4096	0.5102	2.1670	0.8829	0.9944	0.7262	1.4844	0.4922	1.6865	2.2469
Palmitoleylcarnitine	1.1628	1.1070	1.4353	1.5894	1.5156	1.6021	0.2113	0.3347	1.5215	0.4899	0.5611	0.5151	1.7502	0.3258	1.7227	2.0348
palmitoylcarnitine	1.6095	1.5806	1.8413	2.0198	1.9244	2.0461	0.4216	0.4680	2.2600	0.9266	0.9598	0.7314	2.0693	0.4870	2.1088	2.6214
propionylcarnitine	0.1415	0.0867	0.1759	0.3348	0.2045	0.1432	0.1050	0.1631	1.2668	0.3269	0.5375	0.1621	0.1597	0.1814	0.2204	0.6047
Stearoylcarnitine	0.0086	0.0067	0.0068	0.0100	0.0123	0.1049	0.0009	0.0030	0.0604	0.0028	0.0082	0.0054	0.0981	0.0016	0.1738	0.0379
Tetradecadiencarnitine	0.0084	0.0041	0.0054	0.0154	0.0076	0.0046	0.0019	0.0055	0.0182	0.0073	0.0079	0.0079	0.0077	0.0042	0.0110	0.0540
Tetradecenoylcarnitine	0.1797	0.0725	0.1045	0.2316	0.1672	0.1285	0.0312	0.0659	0.2898	0.0714	0.0980	0.0818	0.1404	0.0524	0.2087	0.6864
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	moverz_quant	ri	ri_type	pubchem_id	inchi_key	kegg_id	other_id	other_id_type
3-hydroxylauroylcarnitine				71464535			C12-OH	UM_Target_ID
3-Hydroxylinoleoylcarnitine				78076335			C18:2-OH	UM_Target_ID
3-hydroxymyristoylcarnitine				71464541			C14-OH	UM_Target_ID
3-Hydroxypalmitoylcarnitine				71464553			C16-OH	UM_Target_ID
Acetylcarnitine				1		C02571	C2	UM_Target_ID
arachidonoylcarnitine				53477832			C20:4	UM_Target_ID
butyrylcarnitine				213144		C02862	C4	UM_Target_ID
Decanoylcarnitine				11953821		C03299	C10:0	UM_Target_ID
decenoylcarnitine				54074509			C10:1	UM_Target_ID
Dodecenoylcarnitine				53481671			C12:1	UM_Target_ID
Glutarylcarnitine				53481622			C5:0-DC	UM_Target_ID
Hexanoylcarnitine				6426853			C6:0	UM_Target_ID
icosadienoylcarnitine				71464509			C20:2	UM_Target_ID
Icosanoylcarnitine				53477833			C20	UM_Target_ID
icosatrienoylcarnitine							C20:3	UM_Target_ID
icoseneoylcarnitine				71464507			C20:1	UM_Target_ID
isovalerylcarnitine				6426851			C5:0	UM_Target_ID
lauroylcarnitine				10427569			C12:0	UM_Target_ID
L-Carnitine				288			L-Carnitine	UM_Target_ID
Linoelaidyl carnitine				53477834			C18:2	UM_Target_ID
myristoylcarnitine				53477791			C14:0	UM_Target_ID
octanoylcarnitine				123701			C8:0	UM_Target_ID
Octenoylcarnitine				71464472			C8:1	UM_Target_ID
Oleoylcarnitine				6441392			C18:1	UM_Target_ID
Palmitoleylcarnitine				71464547			C16:1	UM_Target_ID
palmitoylcarnitine				11953816		C02990	C16:0	UM_Target_ID
propionylcarnitine				188824		C03017	C3	UM_Target_ID
Stearoylcarnitine				52922056			C18:0	UM_Target_ID
Tetradecadiencarnitine				71464539			C14:2	UM_Target_ID
Tetradecenoylcarnitine				22833575			C14:1	UM_Target_ID
METABOLITES_END
#END