#METABOLOMICS WORKBENCH STUDY_ID:ST000283 ANALYSIS_ID:AN000451 PROJECT_ID:PR000140 VERSION 1 CREATED_ON 2016-09-17 #PROJECT PR:PROJECT_TITLE Human AxP Studies PR:PROJECT_TYPE Glycolysis/TCA/Nucleotide analysis (tissue/cells) PR:PROJECT_SUMMARY Plasma Nucleotide/adenosine concentrations PR:INSTITUTE University of Michigan PR:DEPARTMENT Internal Medicine-Cardiology PR:LABORATORY Visovatti Lab PR:LAST_NAME Visovatti PR:FIRST_NAME Scott PR:ADDRESS Ann Arbor, MI PR:EMAIL shv@med.umich.edu PR:PHONE 734-657-6917 #STUDY ST:STUDY_TITLE Plasma Nucleotide/adenosine concentrations (Human AxP Batch 4) ST:STUDY_TYPE Glycolysis/TCA/Nucleotide analysis (tissue/cells) ST:STUDY_SUMMARY This experiment was performed using the following cohorts: 1) healthy controls, ST:STUDY_SUMMARY patients with scleroderma at low risk for pulmonary hypertension, 3) pateints ST:STUDY_SUMMARY scleroderma at high risk for pulmonary hypertension. Whole blood was drawn ST:STUDY_SUMMARY into stop soilution (1:1 ratio) at rest and again at peak exercise for each ST:STUDY_SUMMARY subject. ST:INSTITUTE University of Michigan ST:DEPARTMENT Internal Medicine-Cardiology ST:LABORATORY Visovatti Lab ST:LAST_NAME Robichaud ST:FIRST_NAME Patrick ST:ADDRESS Ann Arbor, MI ST:EMAIL probicha@umich.edu ST:PHONE 734-936-7603 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 15 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:SPECIES_GROUP Human #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0011567 S00017057 EXERCISE:No | GROUP:Healthy name=53-E SUBJECT_SAMPLE_FACTORS SU0011571 S00017061 EXERCISE:No | GROUP:Healthy name=57-E SUBJECT_SAMPLE_FACTORS SU0011564 S00017053 EXERCISE:No | GROUP:SSc Cath name=63-E SUBJECT_SAMPLE_FACTORS SU0011566 S00017055 EXERCISE:No | GROUP:SSc Cath name=50-R SUBJECT_SAMPLE_FACTORS SU0011569 S00017059 EXERCISE:No | GROUP:SSc Cath name=59-E SUBJECT_SAMPLE_FACTORS SU0011573 S00017063 EXERCISE:No | GROUP:SSc Cath name=49-R SUBJECT_SAMPLE_FACTORS SU0011575 S00017065 EXERCISE:No | GROUP:SSc Cath name=55-E SUBJECT_SAMPLE_FACTORS SU0011577 S00017067 EXERCISE:No | GROUP:SSc Cath name=62-E SUBJECT_SAMPLE_FACTORS SU0011567 S00017056 EXERCISE:Yes | GROUP:Healthy name=53-R SUBJECT_SAMPLE_FACTORS SU0011571 S00017060 EXERCISE:Yes | GROUP:Healthy name=57-R SUBJECT_SAMPLE_FACTORS SU0011564 S00017054 EXERCISE:Yes | GROUP:SSc Cath name=59-R SUBJECT_SAMPLE_FACTORS SU0011569 S00017058 EXERCISE:Yes | GROUP:SSc Cath name=62-R SUBJECT_SAMPLE_FACTORS SU0011573 S00017062 EXERCISE:Yes | GROUP:SSc Cath name=55-R SUBJECT_SAMPLE_FACTORS SU0011575 S00017064 EXERCISE:Yes | GROUP:SSc Cath name=49-E SUBJECT_SAMPLE_FACTORS SU0011577 S00017066 EXERCISE:Yes | GROUP:SSc Cath name=63-R #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Plasma #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Waters Acquity HSS T3 CH:METHODS_ID AQM016 CH:METHODS_FILENAME QQQ-ATP_ADP_AMP_A-update.m.zip #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ACQUISITION_PARAMETERS_FILE QQQ-ATP_ADP_AMP_A-update.m.zip #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6530 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nM MS_METABOLITE_DATA_START Samples S00017057 S00017061 S00017053 S00017055 S00017059 S00017063 S00017065 S00017067 S00017056 S00017060 S00017054 S00017058 S00017062 S00017064 S00017066 Factors EXERCISE:No | GROUP:Healthy EXERCISE:No | GROUP:Healthy EXERCISE:No | GROUP:SSc Cath EXERCISE:No | GROUP:SSc Cath EXERCISE:No | GROUP:SSc Cath EXERCISE:No | GROUP:SSc Cath EXERCISE:No | GROUP:SSc Cath EXERCISE:No | GROUP:SSc Cath EXERCISE:Yes | GROUP:Healthy EXERCISE:Yes | GROUP:Healthy EXERCISE:Yes | GROUP:SSc Cath EXERCISE:Yes | GROUP:SSc Cath EXERCISE:Yes | GROUP:SSc Cath EXERCISE:Yes | GROUP:SSc Cath EXERCISE:Yes | GROUP:SSc Cath Adenosine 65.4635 45.9903 42.3450 43.0796 41.2742 43.2446 53.4714 30.0532 71.2058 46.8618 56.7932 24.1749 36.8962 51.7170 16.5037 ADP 114.7699 238.9578 206.5405 193.0720 91.6454 271.6338 156.7159 224.2924 232.4423 187.9204 235.4674 266.2202 239.5008 146.6297 387.3478 AMP 47.4776 55.5785 69.7165 58.9721 42.5416 104.2914 60.2286 80.9138 85.0405 54.5364 118.4388 78.5573 78.4047 68.8543 70.4987 ATP 88.3558 335.9582 249.5417 179.2092 119.9121 246.0039 186.3683 265.0413 195.5493 263.4465 214.3195 253.4714 276.0918 197.0901 464.5435 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type Adenosine 60961 C00212 Adenosine UM_Target_ID ADP 6022 C00008 ADP UM_Target_ID AMP 6083 C00020 AMP UM_Target_ID ATP 5957 C00002 ATP UM_Target_ID METABOLITES_END #END