#METABOLOMICS WORKBENCH araskind_20151211_9257891_mwtab.txt DATATRACK_ID:453 efahy_20151228_064502 STUDY_ID:ST000295 ANALYSIS_ID:AN000472 PROJECT_ID:PR000219 VERSION 1 CREATED_ON December 28, 2015, 6:45 am #PROJECT PR:PROJECT_TITLE Metabolic regulation by PARP-1 in myofibroblast differentiation and fibrosis PR:PROJECT_SUMMARY Analysis of wild type lung tissue after saline or bleomycin treatment PR:INSTITUTE University of Michigan PR:LAST_NAME Hu PR:FIRST_NAME Biao PR:ADDRESS Ann Arbor, MI PR:EMAIL biaohu@med.umich.edu PR:PHONE 734-763-5731 #STUDY ST:STUDY_TITLE Metabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human ST:STUDY_TITLE Normal Lung Fiboblasts with TGFbeta and Fizz2 treatment ST:STUDY_SUMMARY Glycolysis/TCA/Nucleotide analysis and NAD+ and related metabolite analysis for ST:STUDY_SUMMARY all samples ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 6 ST:TOTAL_SUBJECTS 35 #SUBJECT SU:SUBJECT_TYPE Animal/Human SU:SUBJECT_SPECIES Mus musculus;Homo sapiens #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0005146 S00016590 Species:Mus musculus | Genotype:WT | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0005147 S00016591 Species:Mus musculus | Genotype:WT | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0005148 S00016592 Species:Mus musculus | Genotype:WT | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0005149 S00016593 Species:Mus musculus | Genotype:WT | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0005150 S00016594 Species:Mus musculus | Genotype:WT | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0005151 S00016595 Species:Mus musculus | Genotype:WT | Treatment:bleomycin SUBJECT_SAMPLE_FACTORS SU0005152 S00016596 Species:Mus musculus | Genotype:WT | Treatment:bleomycin SUBJECT_SAMPLE_FACTORS SU0005153 S00016597 Species:Mus musculus | Genotype:WT | Treatment:bleomycin SUBJECT_SAMPLE_FACTORS SU0005154 S00016598 Species:Mus musculus | Genotype:WT | Treatment:bleomycin SUBJECT_SAMPLE_FACTORS SU0005155 S00016599 Species:Mus musculus | Genotype:WT | Treatment:bleomycin SUBJECT_SAMPLE_FACTORS SU0005156 S00016600 Species:Mus musculus | Genotype:PARP1 knockout | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0005157 S00016601 Species:Mus musculus | Genotype:PARP1 knockout | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0005158 S00016602 Species:Mus musculus | Genotype:PARP1 knockout | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0005159 S00016603 Species:Mus musculus | Genotype:PARP1 knockout | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0005160 S00016604 Species:Mus musculus | Genotype:PARP1 knockout | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0005161 S00016605 Species:Mus musculus | Genotype:PARP1 knockout | Treatment:bleomycin SUBJECT_SAMPLE_FACTORS SU0005162 S00016606 Species:Mus musculus | Genotype:PARP1 knockout | Treatment:bleomycin SUBJECT_SAMPLE_FACTORS SU0005163 S00016607 Species:Mus musculus | Genotype:PARP1 knockout | Treatment:bleomycin SUBJECT_SAMPLE_FACTORS SU0005164 S00016608 Species:Mus musculus | Genotype:PARP1 knockout | Treatment:bleomycin SUBJECT_SAMPLE_FACTORS SU0005165 S00016609 Species:Mus musculus | Genotype:PARP1 knockout | Treatment:bleomycin SUBJECT_SAMPLE_FACTORS SU0005166 S00016610 Species:Homo sapiens | Genotype:WT | Treatment:None SUBJECT_SAMPLE_FACTORS SU0005167 S00016611 Species:Homo sapiens | Genotype:WT | Treatment:None SUBJECT_SAMPLE_FACTORS SU0005168 S00016612 Species:Homo sapiens | Genotype:WT | Treatment:None SUBJECT_SAMPLE_FACTORS SU0005169 S00016613 Species:Homo sapiens | Genotype:WT | Treatment:None SUBJECT_SAMPLE_FACTORS SU0005170 S00016614 Species:Homo sapiens | Genotype:WT | Treatment:None SUBJECT_SAMPLE_FACTORS SU0005171 S00016615 Species:Homo sapiens | Genotype:WT | Treatment:TGFb SUBJECT_SAMPLE_FACTORS SU0005172 S00016616 Species:Homo sapiens | Genotype:WT | Treatment:TGFb SUBJECT_SAMPLE_FACTORS SU0005173 S00016617 Species:Homo sapiens | Genotype:WT | Treatment:TGFb SUBJECT_SAMPLE_FACTORS SU0005174 S00016618 Species:Homo sapiens | Genotype:WT | Treatment:TGFb SUBJECT_SAMPLE_FACTORS SU0005175 S00016619 Species:Homo sapiens | Genotype:WT | Treatment:TGFb SUBJECT_SAMPLE_FACTORS SU0005176 S00016620 Species:Homo sapiens | Genotype:WT | Treatment:Fizz2 SUBJECT_SAMPLE_FACTORS SU0005177 S00016621 Species:Homo sapiens | Genotype:WT | Treatment:Fizz2 SUBJECT_SAMPLE_FACTORS SU0005178 S00016622 Species:Homo sapiens | Genotype:WT | Treatment:Fizz2 SUBJECT_SAMPLE_FACTORS SU0005179 S00016623 Species:Homo sapiens | Genotype:WT | Treatment:Fizz2 SUBJECT_SAMPLE_FACTORS SU0005180 S00016624 Species:Homo sapiens | Genotype:WT | Treatment:Fizz2 #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Cells #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME Glycolysis-TCA-nucleotides-20150324.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1260 CH:COLUMN_NAME Waters XBridge C18 (50 x 2.1mm, 3um) CH:METHODS_FILENAME Ceramides-20150305.docx CH:METHODS_ID DQM0152 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Ceramides MS(+) MS:INSTRUMENT_NAME Agilent 6410A QQQ MS:INSTRUMENT_NAME Agilent 6410A QQQ #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS ng/ug protein MS_METABOLITE_DATA_START Samples S00016590 S00016591 S00016592 S00016593 S00016594 S00016595 S00016596 S00016597 S00016598 S00016599 S00016600 S00016601 S00016602 S00016603 S00016604 S00016605 S00016606 S00016607 S00016608 S00016609 S00016610 S00016611 S00016612 S00016613 S00016614 S00016615 S00016616 S00016617 S00016618 S00016619 S00016620 S00016621 S00016622 S00016623 S00016624 Factors Species:Mus musculus | Genotype:WT | Treatment:saline Species:Mus musculus | Genotype:WT | Treatment:saline Species:Mus musculus | Genotype:WT | Treatment:saline Species:Mus musculus | Genotype:WT | Treatment:saline Species:Mus musculus | Genotype:WT | Treatment:saline Species:Mus musculus | Genotype:WT | Treatment:bleomycin Species:Mus musculus | Genotype:WT | Treatment:bleomycin Species:Mus musculus | Genotype:WT | Treatment:bleomycin Species:Mus musculus | Genotype:WT | Treatment:bleomycin Species:Mus musculus | Genotype:WT | Treatment:bleomycin Species:Mus musculus | Genotype:PARP1 knockout | Treatment:saline Species:Mus musculus | Genotype:PARP1 knockout | Treatment:saline Species:Mus musculus | Genotype:PARP1 knockout | Treatment:saline Species:Mus musculus | Genotype:PARP1 knockout | Treatment:saline Species:Mus musculus | Genotype:PARP1 knockout | Treatment:saline Species:Mus musculus | Genotype:PARP1 knockout | Treatment:bleomycin Species:Mus musculus | Genotype:PARP1 knockout | Treatment:bleomycin Species:Mus musculus | Genotype:PARP1 knockout | Treatment:bleomycin Species:Mus musculus | Genotype:PARP1 knockout | Treatment:bleomycin Species:Mus musculus | Genotype:PARP1 knockout | Treatment:bleomycin Species:Homo sapiens | Genotype:WT | Treatment:None Species:Homo sapiens | Genotype:WT | Treatment:None Species:Homo sapiens | Genotype:WT | Treatment:None Species:Homo sapiens | Genotype:WT | Treatment:None Species:Homo sapiens | Genotype:WT | Treatment:None Species:Homo sapiens | Genotype:WT | Treatment:TGFb Species:Homo sapiens | Genotype:WT | Treatment:TGFb Species:Homo sapiens | Genotype:WT | Treatment:TGFb Species:Homo sapiens | Genotype:WT | Treatment:TGFb Species:Homo sapiens | Genotype:WT | Treatment:TGFb Species:Homo sapiens | Genotype:WT | Treatment:Fizz2 Species:Homo sapiens | Genotype:WT | Treatment:Fizz2 Species:Homo sapiens | Genotype:WT | Treatment:Fizz2 Species:Homo sapiens | Genotype:WT | Treatment:Fizz2 Species:Homo sapiens | Genotype:WT | Treatment:Fizz2 Cer C14 93.288 101.353 103.542 125.486 140.060 154.471 201.662 155.359 73.501 162.947 135.161 129.968 98.227 132.268 152.251 161.141 111.575 106.159 152.067 101.588 155.382 261.432 378.889 385.095 237.200 300.852 322.175 297.417 251.528 200.446 215.256 277.997 274.900 492.692 411.681 Cer C16 156.292 127.118 179.486 190.264 208.758 237.578 251.387 223.935 129.219 195.350 259.262 194.173 155.850 199.311 237.022 199.104 137.600 131.858 198.442 162.748 155.953 307.779 383.478 454.916 318.123 357.642 311.316 368.992 304.137 264.793 290.305 356.363 247.555 497.786 494.510 Cer C18 63.789 63.740 76.727 86.257 89.406 91.469 82.874 89.978 56.767 67.920 98.494 60.876 61.456 72.299 96.954 69.014 57.414 54.886 86.672 78.614 99.899 172.728 222.884 264.461 185.460 217.412 201.513 213.863 166.862 145.998 171.378 213.614 160.240 326.282 295.957 Cer C18:1 2.000 1.935 2.312 2.793 2.551 3.645 2.068 2.533 1.512 1.296 3.024 1.615 1.654 1.787 2.328 1.984 1.217 1.052 1.989 2.398 2.840 5.707 8.151 8.302 6.284 6.760 6.662 8.066 5.510 5.259 6.104 7.789 5.223 10.173 9.416 Cer C20 70.328 69.333 90.764 104.009 108.344 108.613 96.793 92.643 69.257 88.966 110.143 69.808 70.775 74.115 89.809 90.507 79.281 86.101 122.261 99.257 110.621 228.758 234.373 328.060 242.337 267.997 250.882 308.470 243.249 186.254 225.505 288.603 217.809 410.320 385.374 Cer C22 249246.300 197007.476 515291.883 355460.976 464656.841 365254.181 510307.001 312255.895 315255.764 488964.969 736807.099 493406.892 282547.056 387942.959 458349.476 496853.384 219198.867 343588.209 658433.474 491547.356 234580.372 428701.027 728329.987 721773.987 614381.348 492752.563 666927.938 859299.330 607305.494 450409.594 616280.493 684041.466 570624.478 881893.200 633436.779 Cer C24 148.883 125.673 200.816 148.731 217.472 210.894 309.305 167.150 124.048 295.245 234.005 192.946 115.978 180.340 212.509 219.166 244.737 138.430 279.632 203.872 136.343 410.319 578.833 644.635 371.498 445.406 465.718 538.066 416.276 179.728 181.825 225.026 214.018 674.257 397.511 Cer C24:1 943191.087 587489.776 1451552.522 1270213.796 1366797.675 1353068.120 1727224.089 1147270.861 1020504.433 1376437.803 2033184.497 1328641.249 987074.190 1132915.244 1399355.663 1393113.445 714921.394 1010625.816 1770983.500 1440687.416 550532.395 988602.202 1494584.401 1470959.388 1470856.218 1283520.304 1542765.927 1963191.691 1381850.103 1331870.778 1426053.842 1578535.966 1262880.766 1656101.332 1423790.665 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name PubChem CID Cer C14 5282310 Cer C16 5283564 Cer C18 5283565 Cer C18:1 5283563 Cer C20 5283566 Cer C22 5283567 Cer C24 5283571 Cer C24:1 5283568 METABOLITES_END #END