#METABOLOMICS WORKBENCH araskind_20151211_9487131_mwtab.txt DATATRACK_ID:456 efahy_20151228_092507 STUDY_ID:ST000296 ANALYSIS_ID:AN000474 PROJECT_ID:PR000237 VERSION 1 CREATED_ON December 28, 2015, 9:25 am #PROJECT PR:PROJECT_TITLE Brain-Immune-Gut Interaction Studies PR:PROJECT_SUMMARY Brain-Immune-Gut Interaction in Chronic Mild Stress PR:INSTITUTE University of Virginia PR:DEPARTMENT Neuroscience PR:LABORATORY Kipnis Lab PR:LAST_NAME Kipnis PR:FIRST_NAME Jonathan PR:ADDRESS Charlottesville, VA PR:EMAIL kipnis@virginia.edu PR:PHONE 434-982-3858 #STUDY ST:STUDY_TITLE Chronic mild stress and Lactobacillus experiments on mice ST:STUDY_SUMMARY Mice were devided into three groups (Naive untreated, Stressed untreated, ST:STUDY_SUMMARY Stressed + Lacto). The stressed groups were subjected to unpredictable chronic ST:STUDY_SUMMARY mild stress for seven weeks. Three weeks into the protocol, the +Lacto groups ST:STUDY_SUMMARY were administered probiotic Lactobacillus daily. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 16 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0005237 S00016738 Lactobacillus:N | Stress:N SUBJECT_SAMPLE_FACTORS SU0005238 S00016739 Lactobacillus:N | Stress:N SUBJECT_SAMPLE_FACTORS SU0005239 S00016740 Lactobacillus:N | Stress:N SUBJECT_SAMPLE_FACTORS SU0005240 S00016741 Lactobacillus:N | Stress:N SUBJECT_SAMPLE_FACTORS SU0005241 S00016742 Lactobacillus:N | Stress:N SUBJECT_SAMPLE_FACTORS SU0005242 S00016743 Lactobacillus:N | Stress:Y SUBJECT_SAMPLE_FACTORS SU0005243 S00016744 Lactobacillus:N | Stress:Y SUBJECT_SAMPLE_FACTORS SU0005244 S00016745 Lactobacillus:N | Stress:Y SUBJECT_SAMPLE_FACTORS SU0005245 S00016746 Lactobacillus:N | Stress:Y SUBJECT_SAMPLE_FACTORS SU0005246 S00016747 Lactobacillus:N | Stress:Y SUBJECT_SAMPLE_FACTORS SU0005247 S00016748 Lactobacillus:Y | Stress:Y SUBJECT_SAMPLE_FACTORS SU0005248 S00016749 Lactobacillus:Y | Stress:Y SUBJECT_SAMPLE_FACTORS SU0005249 S00016750 Lactobacillus:Y | Stress:Y SUBJECT_SAMPLE_FACTORS SU0005250 S00016751 Lactobacillus:Y | Stress:Y SUBJECT_SAMPLE_FACTORS SU0005251 S00016752 Lactobacillus:Y | Stress:Y SUBJECT_SAMPLE_FACTORS SU9999996 S00017167 Lactobacillus:POOLED | Stress:POOLED #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Serum #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME ShortChainFattyAcids-20150305.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890A CH:COLUMN_NAME Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um) CH:METHODS_FILENAME WAXPLUS_100-200C_2_SIM.M #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:MS_COMMENTS GCMS method MS:INSTRUMENT_NAME Agilent 5975C MS:INSTRUMENT_NAME Agilent 5975C MS #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples S00016738 S00016739 S00016740 S00016741 S00016742 S00016743 S00016744 S00016745 S00016746 S00016747 S00016748 S00016749 S00016750 S00016751 S00016752 Factors Lactobacillus:N | Stress:N Lactobacillus:N | Stress:N Lactobacillus:N | Stress:N Lactobacillus:N | Stress:N Lactobacillus:N | Stress:N Lactobacillus:N | Stress:Y Lactobacillus:N | Stress:Y Lactobacillus:N | Stress:Y Lactobacillus:N | Stress:Y Lactobacillus:N | Stress:Y Lactobacillus:Y | Stress:Y Lactobacillus:Y | Stress:Y Lactobacillus:Y | Stress:Y Lactobacillus:Y | Stress:Y Lactobacillus:Y | Stress:Y acetic acid 6072.723 3470.08 1152.224 2396.397 2159.74 4137.853 2449.86 2047.229 2427.287 2628.231 1687.438 2894.744 2070.666 987.787 1077.45 butyric acid 33.494 25.51 16.505 66.207 36.846 32.786 21.664 17.511 15.958 17.531 17.286 22.716 177.01 14.269 89.634 heptanoic acid 2.974 0 0 3.551 0 3.115 3.591 0 0 0 0 0 10.332 0 4.265 hexanoic acid 4.545 3.675 3.818 7.815 3.752 5.404 7.428 5.022 3.145 3.633 4.217 3.594 28.825 2.331 9.412 iso-butyric acid 50.678 36.645 29.595 33.792 28.488 32.147 32.945 30.028 27.93 36.146 28.158 26.364 41.203 23.818 24.503 isovaleric acid 5.626 7.25 5.115 16.705 3.681 7.582 5.376 4.476 3.891 5.123 4.13 5.129 92.921 3.07 25.273 propionic acid 129.95 124.606 81.927 137.829 109.818 121.756 102.759 87.732 74.897 94.027 98.216 95.196 304.038 83.13 154.179 valeric acid 9.704 6.91 2.506 25.18 3.628 10.277 10.367 4.786 2.982 2.164 3.951 5.139 123.288 2.667 35.923 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name PubChem CID acetic acid 176 propionic acid 1032 iso-butyric acid 6590 butyric acid 20975673 isovaleric acid 10430 valeric acid 7991 hexanoic acid 8892 heptanoic acid 8094 METABOLITES_END #END