#METABOLOMICS WORKBENCH efahy_20151228_092507 STUDY_ID:ST000296 ANALYSIS_ID:AN000474 PROJECT_ID:PR000237
VERSION             	1
CREATED_ON             	December 28, 2015, 9:25 am
#PROJECT
PR:PROJECT_TITLE                 	Brain-Immune-Gut Interaction Studies
PR:PROJECT_SUMMARY               	Brain-Immune-Gut Interaction in Chronic Mild Stress
PR:INSTITUTE                     	University of Virginia
PR:DEPARTMENT                    	Neuroscience
PR:LABORATORY                    	Kipnis Lab
PR:LAST_NAME                     	Kipnis
PR:FIRST_NAME                    	Jonathan
PR:ADDRESS                       	Charlottesville, VA
PR:EMAIL                         	kipnis@virginia.edu
PR:PHONE                         	434-982-3858
#STUDY
ST:STUDY_TITLE                   	Chronic mild stress and Lactobacillus experiments on mice
ST:STUDY_SUMMARY                 	Mice were devided into three groups (Naive untreated, Stressed untreated,
ST:STUDY_SUMMARY                 	Stressed + Lacto). The stressed groups were subjected to unpredictable chronic
ST:STUDY_SUMMARY                 	mild stress for seven weeks. Three weeks into the protocol, the +Lacto groups
ST:STUDY_SUMMARY                 	were administered probiotic Lactobacillus daily.
ST:INSTITUTE                     	University of Michigan
ST:DEPARTMENT                    	Biomedical Research Core Facilities
ST:LABORATORY                    	Metabolomics core
ST:LAST_NAME                     	Kachman
ST:FIRST_NAME                    	Maureen
ST:ADDRESS                       	6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL                         	mkachman@med.umich.edu
ST:PHONE                         	(734) 232-8175
ST:NUM_GROUPS                    	4
ST:TOTAL_SUBJECTS                	16
#SUBJECT
SU:SUBJECT_TYPE                  	Animal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	SU0005237	S00016738	Lactobacillus:N | Stress:N	
SUBJECT_SAMPLE_FACTORS           	SU0005238	S00016739	Lactobacillus:N | Stress:N	
SUBJECT_SAMPLE_FACTORS           	SU0005239	S00016740	Lactobacillus:N | Stress:N	
SUBJECT_SAMPLE_FACTORS           	SU0005240	S00016741	Lactobacillus:N | Stress:N	
SUBJECT_SAMPLE_FACTORS           	SU0005241	S00016742	Lactobacillus:N | Stress:N	
SUBJECT_SAMPLE_FACTORS           	SU0005242	S00016743	Lactobacillus:N | Stress:Y	
SUBJECT_SAMPLE_FACTORS           	SU0005243	S00016744	Lactobacillus:N | Stress:Y	
SUBJECT_SAMPLE_FACTORS           	SU0005244	S00016745	Lactobacillus:N | Stress:Y	
SUBJECT_SAMPLE_FACTORS           	SU0005245	S00016746	Lactobacillus:N | Stress:Y	
SUBJECT_SAMPLE_FACTORS           	SU0005246	S00016747	Lactobacillus:N | Stress:Y	
SUBJECT_SAMPLE_FACTORS           	SU0005247	S00016748	Lactobacillus:Y | Stress:Y	
SUBJECT_SAMPLE_FACTORS           	SU0005248	S00016749	Lactobacillus:Y | Stress:Y	
SUBJECT_SAMPLE_FACTORS           	SU0005249	S00016750	Lactobacillus:Y | Stress:Y	
SUBJECT_SAMPLE_FACTORS           	SU0005250	S00016751	Lactobacillus:Y | Stress:Y	
SUBJECT_SAMPLE_FACTORS           	SU0005251	S00016752	Lactobacillus:Y | Stress:Y	
SUBJECT_SAMPLE_FACTORS           	SU9999996	S00017167	Lactobacillus:POOLED | Stress:POOLED	
#COLLECTION
CO:COLLECTION_SUMMARY            	-
CO:SAMPLE_TYPE                   	Serum
#TREATMENT
TR:TREATMENT_SUMMARY             	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	-
SP:SAMPLEPREP_PROTOCOL_FILENAME  	ShortChainFattyAcids-20150305.docx
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	GC
CH:INSTRUMENT_NAME               	Agilent 7890A
CH:COLUMN_NAME                   	Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um)
CH:METHODS_FILENAME              	WAXPLUS_100-200C_2_SIM.M
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_TYPE               	Single quadrupole
MS:MS_TYPE                       	EI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	GCMS method
MS:INSTRUMENT_NAME               	Agilent 5975C
MS:INSTRUMENT_NAME               	Agilent 5975C MS
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	uM
MS_METABOLITE_DATA_START
Samples	S00016738	S00016739	S00016740	S00016741	S00016742	S00016743	S00016744	S00016745	S00016746	S00016747	S00016748	S00016749	S00016750	S00016751	S00016752
Factors	Lactobacillus:N | Stress:N	Lactobacillus:N | Stress:N	Lactobacillus:N | Stress:N	Lactobacillus:N | Stress:N	Lactobacillus:N | Stress:N	Lactobacillus:N | Stress:Y	Lactobacillus:N | Stress:Y	Lactobacillus:N | Stress:Y	Lactobacillus:N | Stress:Y	Lactobacillus:N | Stress:Y	Lactobacillus:Y | Stress:Y	Lactobacillus:Y | Stress:Y	Lactobacillus:Y | Stress:Y	Lactobacillus:Y | Stress:Y	Lactobacillus:Y | Stress:Y
acetic acid	6072.723	3470.08	1152.224	2396.397	2159.74	4137.853	2449.86	2047.229	2427.287	2628.231	1687.438	2894.744	2070.666	987.787	1077.45
butyric acid	33.494	25.51	16.505	66.207	36.846	32.786	21.664	17.511	15.958	17.531	17.286	22.716	177.01	14.269	89.634
heptanoic acid	2.974	0	0	3.551	0	3.115	3.591	0	0	0	0	0	10.332	0	4.265
hexanoic acid	4.545	3.675	3.818	7.815	3.752	5.404	7.428	5.022	3.145	3.633	4.217	3.594	28.825	2.331	9.412
iso-butyric acid	50.678	36.645	29.595	33.792	28.488	32.147	32.945	30.028	27.93	36.146	28.158	26.364	41.203	23.818	24.503
isovaleric acid	5.626	7.25	5.115	16.705	3.681	7.582	5.376	4.476	3.891	5.123	4.13	5.129	92.921	3.07	25.273
propionic acid	129.95	124.606	81.927	137.829	109.818	121.756	102.759	87.732	74.897	94.027	98.216	95.196	304.038	83.13	154.179
valeric acid	9.704	6.91	2.506	25.18	3.628	10.277	10.367	4.786	2.982	2.164	3.951	5.139	123.288	2.667	35.923
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	PubChem CID
acetic acid	176
propionic acid	1032
iso-butyric acid	6590
butyric acid	20975673
isovaleric acid	10430
valeric acid	7991
hexanoic acid	8892
heptanoic acid	8094
METABOLITES_END
#END