#METABOLOMICS WORKBENCH araskind_20151211_9191311_mwtab.txt DATATRACK_ID:457 efahy_20151228_102042 STUDY_ID:ST000297 ANALYSIS_ID:AN000475 PROJECT_ID:PR000238 VERSION 1 CREATED_ON December 28, 2015, 10:20 am #PROJECT PR:PROJECT_TITLE Intestinal lipid studies PR:PROJECT_SUMMARY Intestinal lipid oxidation after VSG PR:INSTITUTE University of Michigan PR:DEPARTMENT Surgery PR:LABORATORY Roelofs Lab PR:LAST_NAME Roelofs PR:FIRST_NAME Karen PR:ADDRESS Ann Arbor, MI PR:EMAIL roelofs@med.umich.edu PR:PHONE 734-763-8526 #STUDY ST:STUDY_TITLE Intestinal lipid oxidation after VSG ST:STUDY_SUMMARY Postprandial lipids are lower after vertical sleeve gastrectomy (VSG) surgery ST:STUDY_SUMMARY and this efect is independent of lipid absorption and chylomicron production. ST:STUDY_SUMMARY This poses a question of where the lipids are going. In order to test the ST:STUDY_SUMMARY hypothesis that intestinal oxidation of lipids is increased, Sham or VSG animals ST:STUDY_SUMMARY were gavaged with glycerol trioleate or water and sacrificed 2h later. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 34 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10114 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0011583 S00017088 Surgery Type:Sham | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011584 S00017089 Surgery Type:Sham | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011585 S00017090 Surgery Type:Sham | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011586 S00017091 Surgery Type:Sham | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011587 S00017092 Surgery Type:Sham | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011588 S00017093 Surgery Type:Sham | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011589 S00017094 Surgery Type:Sham | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011590 S00017095 Surgery Type:Sham | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011591 S00017096 Surgery Type:Sham | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011592 S00017097 Surgery Type:Sham | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011593 S00017098 Surgery Type:Sham | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011594 S00017099 Surgery Type:Sham | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011595 S00017100 Surgery Type:Sham | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011596 S00017101 Surgery Type:Sham | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011597 S00017102 Surgery Type:Sham | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011598 S00017103 Surgery Type:Sham | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011599 S00017104 Surgery Type:Sham | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011600 S00017105 Surgery Type:VSG | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011601 S00017106 Surgery Type:VSG | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011602 S00017107 Surgery Type:VSG | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011603 S00017108 Surgery Type:VSG | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011604 S00017109 Surgery Type:VSG | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011605 S00017110 Surgery Type:VSG | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011606 S00017111 Surgery Type:VSG | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011607 S00017112 Surgery Type:VSG | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011608 S00017113 Surgery Type:VSG | Treatment:glycerol trioleate gavage SUBJECT_SAMPLE_FACTORS SU0011609 S00017114 Surgery Type:VSG | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011610 S00017115 Surgery Type:VSG | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011611 S00017116 Surgery Type:VSG | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011612 S00017117 Surgery Type:VSG | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011613 S00017118 Surgery Type:VSG | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011614 S00017119 Surgery Type:VSG | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011615 S00017120 Surgery Type:VSG | Treatment:water gavage SUBJECT_SAMPLE_FACTORS SU0011616 S00017121 Surgery Type:VSG | Treatment:water gavage #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Tissues #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME AcylCarnitines - 20150305.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1260 CH:COLUMN_NAME Waters X BridgeC18 1mm x 50mm CH:METHODS_FILENAME QM_004XBridge2mm_ACAR_MRM20150412.m #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ANALYSIS_PROTOCOL_FILE QM_004XBridge2mm_ACAR_MRM20150412.m #MS MS:MS_COMMENTS - MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:INSTRUMENT_NAME Agilent 6410 QQQ MS:INSTRUMENT_NAME Agilent 6410 QQQ #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/mg MS_METABOLITE_DATA_START Samples S00017088 S00017089 S00017090 S00017091 S00017092 S00017093 S00017094 S00017095 S00017096 S00017097 S00017098 S00017099 S00017100 S00017101 S00017102 S00017103 S00017104 S00017105 S00017106 S00017107 S00017108 S00017109 S00017110 S00017111 S00017112 S00017113 S00017114 S00017115 S00017116 S00017117 S00017118 S00017119 S00017120 Factors Surgery Type:Sham | Treatment:glycerol trioleate gavage Surgery Type:Sham | Treatment:glycerol trioleate gavage Surgery Type:Sham | Treatment:glycerol trioleate gavage Surgery Type:Sham | Treatment:glycerol trioleate gavage Surgery Type:Sham | Treatment:glycerol trioleate gavage Surgery Type:Sham | Treatment:glycerol trioleate gavage Surgery Type:Sham | Treatment:glycerol trioleate gavage Surgery Type:Sham | Treatment:glycerol trioleate gavage Surgery Type:Sham | Treatment:water gavage Surgery Type:Sham | Treatment:water gavage Surgery Type:Sham | Treatment:water gavage Surgery Type:Sham | Treatment:water gavage Surgery Type:Sham | Treatment:water gavage Surgery Type:Sham | Treatment:water gavage Surgery Type:Sham | Treatment:water gavage Surgery Type:Sham | Treatment:water gavage Surgery Type:Sham | Treatment:water gavage Surgery Type:VSG | Treatment:glycerol trioleate gavage Surgery Type:VSG | Treatment:glycerol trioleate gavage Surgery Type:VSG | Treatment:glycerol trioleate gavage Surgery Type:VSG | Treatment:glycerol trioleate gavage Surgery Type:VSG | Treatment:glycerol trioleate gavage Surgery Type:VSG | Treatment:glycerol trioleate gavage Surgery Type:VSG | Treatment:glycerol trioleate gavage Surgery Type:VSG | Treatment:glycerol trioleate gavage Surgery Type:VSG | Treatment:glycerol trioleate gavage Surgery Type:VSG | Treatment:water gavage Surgery Type:VSG | Treatment:water gavage Surgery Type:VSG | Treatment:water gavage Surgery Type:VSG | Treatment:water gavage Surgery Type:VSG | Treatment:water gavage Surgery Type:VSG | Treatment:water gavage Surgery Type:VSG | Treatment:water gavage Carnitine 471.9 734.8 464.8 447.2 820.2 663.9 649.9 499.6 417.6 506.5 536.1 444.8 316.5 517.2 456.1 512.3 548.5 661.5 366.5 492.4 765 713.4 435.9 322.1 483.5 593.4 421.2 340.9 459.2 319.9 533.3 530.6 536.1 CAR(2:0) 222.3 341.1 214.8 186.1 371.1 322.6 287.78 322.2 183.3 195.2 181.1 285.3 137.48 244.8 148.9 253.9 191.8 254.5 116 167 417.2 327.4 108.2 180.6 199.9 227.8 138.6 113.5 199 104.9 125.8 184.5 138.5 CAR(3:0) 4.68 4.94 6.87 5.02 5.77 3.26 5.53 5.48 4.45 3.54 3.84 5.7 4.22 3.74 4.9 4.45 4.25 6.21 3.17 3.93 5.66 8.01 2.71 3.88 3.5 2.87 7.51 2.89 2.85 4.16 4.5 4.55 4.26 CAR(4:0) 11.7 14.6 10.51 10.79 13.9 12.15 14.8 15.46 13.75 13.78 11.39 17 7.48 15.28 11.84 16.66 12.61 17.19 10 11.22 14.19 21 9.22 7.25 11.83 15.45 19.13 11 11.45 8.6 10.42 20.52 12.52 CAR(5:0-DC) 0.1 0.13 0.3 0.19 0.39 0.29 0.19 0.24 0.11 0.1 0.11 0.16 0.13 0.11 0.08 0.2 0.13 0.14 0.08 0.14 0.15 0.16 0.05 0.24 0.16 0.17 0.09 0.09 0.13 0.07 0.1 0.14 0.14 CAR(5:0) 1.82 1.7 2.12 1.67 1.99 2.36 1.91 2.08 3.01 3 2.73 2.9 2.28 3.45 3.04 3.07 2.46 1.97 2.44 2.35 2.35 1.41 2.11 1.66 2.49 3.18 2.74 2.14 1.7 2.81 2.91 3.45 CAR(6:0) 2.84 2.22 1.11 1.42 1.41 1.99 1.56 1.65 2.43 0.95 0.71 1.9 0 1.16 1.47 1.74 1.81 5.29 1.84 1.15 1.67 2.49 1.12 0.77 1.44 2.52 2.82 1.26 2.01 0.99 2.03 2.7 1.62 CAR(8:1) 0.25 0.31 0.18 0.11 0.49 0.18 0.22 0.21 0.06 0.01 0.01 0.01 0.04 0.03 0.03 0.03 0.02 0.34 0.14 0.07 .0.28 0.46 0.09 0.06 0.12 0.09 0.02 0.01 0.01 0.01 0.02 0.03 0.02 CAR(8:0) 1.04 0.29 0.22 0.18 0.3 0.28 0.38 0.35 0.42 0.09 0.09 0.14 0.17 0.2 0.17 0.25 0.18 0.66 0.12 0.18 0.17 0.25 0.18 0.1 0.16 0.22 0.22 0.11 0.13 0.13 0.16 0.24 0.15 CAR(10:1) 0.17 0.18 0.1 0.05 0.28 0.37 0.14 0.12 0.09 0.05 0.08 0.08 0.1 0.09 0.07 0.1 0.08 0.18 0.11 0.07 0.09 0.18 0.12 0.04 0.1 0.19 0.08 0.04 0.09 0.06 0.18 0.08 0.1 CAR(10:0) 0.89 0.6 0.4 0.22 0.61 0.53 0.95 0.44 0.93 0.12 0.17 0.24 0.38 0.62 0.69 0.69 0.69 1.25 0.18 0.16 0.4 0.45 0.58 0.16 0.4 0.49 0.57 0.27 0.31 0.45 0.31 0.48 0.35 CAR(12:0-OH) 0.11 0.1 0.18 0.06 0.22 0.1 0.25 0.11 0.21 0.03 0.08 0.09 0.08 0.16 0.17 0.16 0.2 0.26 0.03 0.05 0.09 0.2 0.1 0.05 0.08 0.09 0.13 0.08 0.1 0.12 0.12 0.21 0.13 CAR(12:1) 0.11 0.13 0.06 0.03 0.13 0.04 0.17 0.12 0.32 0.06 0.25 0.24 0.21 0.1 0.24 0.23 0.15 0.29 0.05 0.05 0.1 0.15 0.05 0.03 0.07 0.18 0.13 0.17 0.21 0.07 0.32 0.18 0.22 CAR(14:2) 0.1 0.12 0.03 0.02 0.09 0.03 0.06 0.08 0.18 0.07 0.19 0.23 0.14 0.06 0.16 0.19 0.09 0.25 0.03 0.04 0.09 0.1 0.02 0.03 0.04 0.12 0.06 0.13 0.17 0.04 0.23 0.13 0.17 CAR(12:0) 0.79 0.66 0.71 0.27 0.63 0.37 0.95 0.66 1.67 0.26 0.81 0.83 1.02 0.85 1.31 1.57 1.15 1.21 0.23 0.26 0.46 0.68 0.56 0.27 0.49 0.7 0.9 0.76 0.55 0.59 0.98 1.16 1.09 CAR(14-OH) 0.12 0.11 0.12 0.07 0.27 0.12 0.15 0.14 0.31 0.07 0.18 0.2 0.12 0.21 0.22 0.28 0.3 0.25 0.05 0.09 0.14 0.22 0.08 0.08 0.15 0.14 0.15 0.13 0.17 0.11 0.22 0.26 0.2 CAR(14:1) 1.12 1.28 0.43 0.39 1.33 0.61 0.83 1.29 1.86 0.46 2.05 1.74 1.36 0.7 1.22 1.63 0.94 1.63 0.4 0.48 0.93 1.46 0.25 0.45 0.51 1.23 0.74 1.19 1.34 0.44 1.77 1.38 1.61 CAR(14:0) 1.12 1.2 1.06 0.84 1.58 0.86 1.13 1.63 2.25 1.02 2.15 2.74 1.68 1.29 1.8 2.6 1.89 1.52 0.77 0.87 1.07 1.86 1.08 0.96 1.15 1.31 1.63 1.48 1.23 1.06 1.47 1.66 1.78 CAR(16:0-OH) 0.38 0.3 0.28 0.22 0.84 0.44 0.48 0.29 0.82 0.24 0.29 0.51 0.23 0.86 0.56 0.74 1.02 0.53 0.11 0.19 0.46 0.39 0.26 0.14 0.79 0.39 0.39 0.34 0.48 0.23 0.5 0.5 0.5 CAR(16:1) 2.36 2.43 1.07 1.24 3.48 1.81 1.96 2.77 3.37 1.57 2.73 2.76 1.81 2.02 2.07 2.64 2.17 2.64 1.18 1.41 1.84 3.13 1.15 1.01 1.68 2.45 1.96 1.86 2.2 1.12 2.98 2.86 2.92 CAR(18:2-OH) 1.24 0.96 0.59 0.62 2.56 1.72 1.14 0.96 0.62 0.17 0.3 0.45 0.21 0.64 0.37 0.53 0.7 0.8 0.32 0.45 0.71 0.72 0.31 0.44 0.78 0.35 0.35 0.18 0.21 0.18 0.29 0.33 0.35 CAR(18:2) 1.62 1.4 0.78 0.76 1.59 1.21 1.23 1.48 0.74 0.51 0.52 0.66 0.34 0.5 0.54 0.73 0.43 0.93 0.42 0.81 1.8 0.63 0.55 0.88 0.7 1.85 0.66 0.59 1.77 0.82 1.94 1.18 0.54 CAR(20:4) 0.11 0.21 0.11 0.09 0.13 0.14 0.17 0.16 0.48 1.09 1.26 0.45 0.42 0.46 0.47 0.46 0.83 0.15 0.06 0.17 0.34 0.18 0.38 0.06 0.4 0.36 0.26 0.6 1.21 0.49 0.79 0.44 0.58 CAR(20:3) 0.04 0.01 0.02 0.03 0.03 0.04 0.04 0.04 0.03 0.06 0.07 0.05 0.03 0.02 0.03 0.02 0.06 0.05 0.02 0.02 0.02 0.02 0.01 0.04 0.02 0.03 0.03 0.03 0.04 0.03 0.03 0.02 0.03 CAR(16:0) 2.43 2.49 3.01 2.03 3.01 1.23 2.93 3.01 4.14 2.7 3.64 5.03 2.79 3.23 2.85 3.68 4.04 3.11 2.09 1.97 2.75 3.2 3 3.52 3.12 3.3 3.57 3.21 3.19 2.17 3.47 3.3 3.66 CAR(18:1) 5.52 5.03 5.2 3.96 6.59 5.54 4.81 5.81 5.72 2.93 4.03 5.97 3.22 4.2 3.94 5 4.87 5.97 4.03 3.31 4.95 5 4.31 7.07 4.71 5.43 4.63 3.83 4.15 2.51 4.96 3.91 4.72 CAR(20:2) 0.49 0.37 0.31 0.43 0.55 0.6 0.57 0.4 0.28 0.22 0.09 0.37 0.08 0.31 0.24 0.32 0.27 0.62 0.25 0.33 0.31 0.31 0.36 0.2 0.44 0.56 0.23 0.21 0.31 0.11 0.24 0.29 0.24 CAR(18:0) 2.15 2.22 3.4 2.42 2.71 2.48 2.28 3.05 3.52 3.01 3.31 4.41 2.52 2.65 2.45 3.54 3.04 2.5 11.84 1.92 2.49 1.95 3.32 3.13 3.41 3.4 3.34 3.23 3.33 2.11 3.6 2.94 3.7 CAR(20:1) 2.7 3 3.42 2.95 3.88 3.77 2.76 3.53 2.06 1.45 1.51 1.56 1.09 2.57 1.49 1.9 1.96 3.25 2.02 2.42 2.72 2.64 2.18 1.86 2.32 3.18 2.42 1.34 1.96 1.44 2.19 1.48 1.75 CAR(20:0) 0.67 0.91 0.81 0.66 1.13 1.04 0.59 0.92 0.71 0.62 0.83 0.55 0.43 1.48 0.54 0.69 1.09 0.51 0.38 0.54 0.84 0.51 0.63 0.41 0.89 0.74 0.85 0.85 0.89 0.55 1.01 0.45 0.75 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name PubChem CID Carnitine 288 CAR(2:0) 1 CAR(3:0) 107738 CAR(4:0) 213144 CAR(5:0-DC) 71317118 CAR(5:0) 6426851 CAR(6:0) 3082079 CAR(8:1) 77908888 CAR(8:0) 123701 CAR(10:1) 57357170 CAR(10:0) 10245190 CAR(12:0-OH) 71464535 CAR(12:1) 87087953 CAR(14:2) 87087940 CAR(12:0) 10427569 CAR(14-OH) 71464541 CAR(14:1) 87088002 CAR(14:0) 6426854 CAR(16:0-OH) 71464553 CAR(16:1) 76040365 CAR(18:2-OH) 71464556 CAR(18:2) 53477834 CAR(20:4) 53477832 CAR(20:3) 54500892 CAR(16:0) 11953816 CAR(18:1) 6441392 CAR(20:2) 71464509 CAR(18:0) 6426855 CAR(20:1) 71464507 CAR(20:0) 53477833 METABOLITES_END #END