#METABOLOMICS WORKBENCH araskind_20151215_9207991_mwtab.txt DATATRACK_ID:459 efahy_20151228_114204 STUDY_ID:ST000298 ANALYSIS_ID:AN000476 PROJECT_ID:PR000239 VERSION 1 CREATED_ON December 28, 2015, 11:42 am #PROJECT PR:PROJECT_TITLE Metabolomics of Psoriasis PR:PROJECT_SUMMARY Metabolomics of Psoriasis PR:INSTITUTE University of Michigan PR:DEPARTMENT Dermatology PR:LABORATORY Gudjonsson Lab PR:LAST_NAME Gudjonsson PR:FIRST_NAME Johann PR:ADDRESS Ann Arbor, MI PR:EMAIL johanng@med.umich.edu PR:PHONE 734-615-4508 #STUDY ST:STUDY_TITLE Analysis of steroid metabolites in psoriasis. ST:STUDY_SUMMARY Analysis of steroid metabolites in tissue of patients with different psoriasis ST:STUDY_SUMMARY status. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 3 ST:TOTAL_SUBJECTS 9 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0011723 S00017314 Psoriasis Status:Psoriasis involved SUBJECT_SAMPLE_FACTORS SU0011723 S00017315 Psoriasis Status:Psoriasis involved SUBJECT_SAMPLE_FACTORS SU0011723 S00017316 Psoriasis Status:Psoriasis involved SUBJECT_SAMPLE_FACTORS SU0011723 S00017317 Psoriasis Status:Psoriasis uninvolved SUBJECT_SAMPLE_FACTORS SU0011723 S00017318 Psoriasis Status:Psoriasis uninvolved SUBJECT_SAMPLE_FACTORS SU0011723 S00017319 Psoriasis Status:Psoriasis uninvolved SUBJECT_SAMPLE_FACTORS SU0011723 S00017320 Psoriasis Status:Normal SUBJECT_SAMPLE_FACTORS SU0011723 S00017321 Psoriasis Status:Normal SUBJECT_SAMPLE_FACTORS SU0011723 S00017322 Psoriasis Status:Normal #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Biopsy #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME Steroids-20150305.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1290 CH:COLUMN_NAME Restek 2.1mm x 50mm 1.9um Pinnacle DB Biphenyl CH:METHODS_FILENAME Steroids-20150305.docx #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE Steroids-20150305.docx #MS MS:MS_COMMENTS - MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE UNSPECIFIED MS:INSTRUMENT_NAME Agilent 6410A QQQ MS:INSTRUMENT_NAME Agilent QQQ 6410A #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pg/mg tissue MS_METABOLITE_DATA_START Samples S00017317 S00017315 S00017322 S00017316 S00017318 S00017319 S00017320 S00017321 S00017314 Factors Psoriasis Status:Psoriasis uninvolved Psoriasis Status:Psoriasis involved Psoriasis Status:Normal Psoriasis Status:Psoriasis involved Psoriasis Status:Psoriasis uninvolved Psoriasis Status:Psoriasis uninvolved Psoriasis Status:Normal Psoriasis Status:Normal Psoriasis Status:Psoriasis involved 11-deoxycortisol 0.570 0.610 0 0.31 2.68 0.26 0.93 3.33 1.43 11-DOC 1.760 0.480 3.74 0.97 3.05 1.29 5.26 9.88 2.05 17-Hydroxyprogesterone 0.180 6.830 29.85 111.35 109.3 53.91 337.19 2126.34 66.98 Androstenedione 0.600 4.620 4.86 3.82 7.71 8.01 17.09 105.27 4.02 Corticosterone 0.570 0.85 0.82 0.63 Cortisol 6.1 3.3 23 24.1 20.1 21.7 130.8 915.4 26.6 Cortisone 8.9 2.8 6 11 14.3 8.4 5.1 16.6 6.2 Progesterone 7.130 2.040 9.29 7.15 32.09 8.36 82.13 136.38 11.35 Testosterone 0.540 0.300 1.1 0.71 1.59 0.29 2.59 2.41 0.46 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name PubChem CID 11-deoxycortisol 440707 11-DOC 6166 17-Hydroxyprogesterone 6238 Androstenedione 6128 Corticosterone 5753 Cortisol 5754 Cortisone 222786 Progesterone 5994 Testosterone 6013 METABOLITES_END #END