#METABOLOMICS WORKBENCH araskind_20151218_9583381_mwtab.txt DATATRACK_ID:467 efahy_20151229_094644 STUDY_ID:ST000304 ANALYSIS_ID:AN000483 PROJECT_ID:PR000244
VERSION             	1
CREATED_ON             	December 29, 2015, 9:46 am
#PROJECT
PR:PROJECT_TITLE                 	Early in life exposure studies
PR:PROJECT_SUMMARY               	Early in life exposures affect innate immune cells activation
PR:INSTITUTE                     	University of Michigan
PR:DEPARTMENT                    	Pediatrics
PR:LABORATORY                    	Hershenson Lab
PR:LAST_NAME                     	Popova
PR:FIRST_NAME                    	Antonia
PR:ADDRESS                       	Ann Arbor, MI
PR:EMAIL                         	antoniap@med.umich.edu
PR:PHONE                         	734-764-6736
#STUDY
ST:STUDY_TITLE                   	Early in life exposure studies on human and mouse samples
ST:STUDY_SUMMARY                 	Experiment1: 2 day old baby mice were exposed to hyperoxia (75% O2) continuously
ST:STUDY_SUMMARY                 	for 7 days. Control baby mice were housed in room air (normoxia). Plasma and
ST:STUDY_SUMMARY                 	bronchoalveolar lavage fluid (BALF) were harvested after 7 days of exposure (on
ST:STUDY_SUMMARY                 	Day of life 9). Experiment2: 2 day old baby mice were exposed to room air or
ST:STUDY_SUMMARY                 	hyperoxia for 14 days and subsequently treated with RV1 or sham.Plasma was
ST:STUDY_SUMMARY                 	collected 5 days after treatment. Human tracheal aspirates were collected from
ST:STUDY_SUMMARY                 	prematurely born infants undergoing mechanical ventilation for respiratory
ST:STUDY_SUMMARY                 	distress syndrome in the first week of life.Tracheal aspirate supernatants are
ST:STUDY_SUMMARY                 	submitted for the assay. We would like to measure adenosine, AMP, ADP and ATP
ST:STUDY_SUMMARY                 	levels.
ST:INSTITUTE                     	University of Michigan
ST:DEPARTMENT                    	Biomedical Research Core Facilities
ST:LABORATORY                    	Metabolomics core
ST:LAST_NAME                     	Kachman
ST:FIRST_NAME                    	Maureen
ST:ADDRESS                       	6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL                         	mkachman@med.umich.edu
ST:PHONE                         	(734) 232-8175
ST:NUM_GROUPS                    	7
ST:TOTAL_SUBJECTS                	52
#SUBJECT
SU:SUBJECT_TYPE                  	Human/animal
SU:SUBJECT_SPECIES               	Mus musculus;Homo sapiens
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	SU0011995	S00017890	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0011996	S00017891	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0011997	S00017892	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0011998	S00017893	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0011999	S00017894	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012000	S00017895	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012001	S00017896	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012002	S00017897	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0011995	S00017898	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0011996	S00017899	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0011997	S00017900	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0011998	S00017901	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0011999	S00017902	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0012000	S00017903	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0012001	S00017904	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0012002	S00017905	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0012003	S00017906	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0012004	S00017907	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0012005	S00017908	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0012006	S00017909	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0012007	S00017910	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	
SUBJECT_SAMPLE_FACTORS           	SU0012003	S00017911	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012004	S00017912	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012005	S00017913	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012006	S00017914	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012007	S00017915	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012008	S00017916	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012009	S00017917	Hyperoxia exposure:n | RV infection:n | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012010	S00017918	Hyperoxia exposure:n | RV infection:n | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012011	S00017919	Hyperoxia exposure:n | RV infection:n | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012012	S00017920	Hyperoxia exposure:n | RV infection:n | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012013	S00017921	Hyperoxia exposure:y | RV infection:n | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012014	S00017922	Hyperoxia exposure:y | RV infection:n | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012015	S00017923	Hyperoxia exposure:y | RV infection:n | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012016	S00017924	Hyperoxia exposure:y | RV infection:y | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012017	S00017925	Hyperoxia exposure:n | RV infection:y | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012018	S00017926	Hyperoxia exposure:n | RV infection:y | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012019	S00017927	Hyperoxia exposure:n | RV infection:y | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012020	S00017928	Hyperoxia exposure:n | RV infection:y | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012021	S00017929	Hyperoxia exposure:y | RV infection:y | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012022	S00017930	Hyperoxia exposure:y | RV infection:y | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012023	S00017931	Hyperoxia exposure:y | RV infection:y | Sample type:Plasma	
SUBJECT_SAMPLE_FACTORS           	SU0012024	S00017932	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	
SUBJECT_SAMPLE_FACTORS           	SU0012025	S00017933	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	
SUBJECT_SAMPLE_FACTORS           	SU0012026	S00017934	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	
SUBJECT_SAMPLE_FACTORS           	SU0012027	S00017935	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	
SUBJECT_SAMPLE_FACTORS           	SU0012028	S00017936	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	
SUBJECT_SAMPLE_FACTORS           	SU0012029	S00017937	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	
SUBJECT_SAMPLE_FACTORS           	SU0012030	S00017938	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	
SUBJECT_SAMPLE_FACTORS           	SU0012031	S00017939	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	
SUBJECT_SAMPLE_FACTORS           	SU0012032	S00017940	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	
SUBJECT_SAMPLE_FACTORS           	SU0012033	S00017941	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	
#COLLECTION
CO:COLLECTION_SUMMARY            	-
CO:SAMPLE_TYPE                   	Bronchoalveolar Lavage Fluid
#TREATMENT
TR:TREATMENT_SUMMARY             	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	-
SP:SAMPLEPREP_PROTOCOL_FILENAME  	Glycolysis-TCA-nucleotides-20150324.docx
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Agilent 1260
CH:COLUMN_NAME                   	Phenomenex Luna NH2 (150 x 1mm, 3um)
CH:METHODS_FILENAME              	QQQ-ATP,ADP,AMP,A-update.m
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	Glycolysis-TCA-nucleotides-20150324.docx
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:INSTRUMENT_NAME               	Agilent 6410 QQQ
MS:INSTRUMENT_NAME               	Agilent QQQ 6410
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	nM
MS_METABOLITE_DATA_START
Samples	S00017890	S00017891	S00017892	S00017893	S00017894	S00017895	S00017896	S00017897	S00017898	S00017899	S00017900	S00017901	S00017902	S00017903	S00017904	S00017905	S00017906	S00017907	S00017908	S00017909	S00017910	S00017911	S00017912	S00017913	S00017914	S00017915	S00017916	S00017917	S00017918	S00017919	S00017920	S00017921	S00017922	S00017923	S00017924	S00017925	S00017926	S00017927	S00017928	S00017929	S00017930	S00017931	S00017932	S00017933	S00017934	S00017935	S00017936	S00017937	S00017938	S00017939	S00017940	S00017941
Factors	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:n | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:y | RV infection:n/a | Sample type:Bronchoalveolar Lavage Fluid	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:n | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:y | RV infection:n/a | Sample type:Plasma	Hyperoxia exposure:n | RV infection:n | Sample type:Plasma	Hyperoxia exposure:n | RV infection:n | Sample type:Plasma	Hyperoxia exposure:n | RV infection:n | Sample type:Plasma	Hyperoxia exposure:n | RV infection:n | Sample type:Plasma	Hyperoxia exposure:y | RV infection:n | Sample type:Plasma	Hyperoxia exposure:y | RV infection:n | Sample type:Plasma	Hyperoxia exposure:y | RV infection:n | Sample type:Plasma	Hyperoxia exposure:y | RV infection:y | Sample type:Plasma	Hyperoxia exposure:n | RV infection:y | Sample type:Plasma	Hyperoxia exposure:n | RV infection:y | Sample type:Plasma	Hyperoxia exposure:n | RV infection:y | Sample type:Plasma	Hyperoxia exposure:n | RV infection:y | Sample type:Plasma	Hyperoxia exposure:y | RV infection:y | Sample type:Plasma	Hyperoxia exposure:y | RV infection:y | Sample type:Plasma	Hyperoxia exposure:y | RV infection:y | Sample type:Plasma	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea	Hyperoxia exposure:n/a | RV infection:n/a | Sample type:Trachea
Adenosine	451.21	384.48	145.60	343.71	410.64	321.17	186.06	3352.85	5587.76	4677.85	4444.76	2096.14	918.16	15609.60	4949.85	6778.91	3552.23	13413.11	5757.74	7643.50	4396.09	6657.44	6168.17	4545.65	10053.27	5467.71	5282.80	9597.40	182.04	92.67	112.08	210.36	235.64	60.15	93.10	203.92	194.00	79.69	1983.26	30936.99	11307.88	6061.51	26859.58	10789.11	16484.98	18779.74	359.24	1028.88	2906.41	2192.16	563.64	646.32
ADP	3667.65	1053.68	237.02	839.01	865.35	360.01	403.88	568.39	2564.21	937.09	1114.42	1588.48	720.34	640.46	415.69	178.73	319.00	175.97	249.84	195.50	715.78	123.47	86.93	59.43	322.01	746.51	143.26	106.24	102.85	99.25	67.46	73.84	76.37	68.86	90.89	77.31	88.77	65.28	2975.89	320.18	4393.75	3345.07	158.13	773.87	1917.18	383.45	2926.50	1848.17	3984.50	1491.40	1304.47	1610.76
AMP	4228.15	847.56	343.16	827.85	902.30	471.51	291.48	1517.69	5950.74	2386.09	2216.87	3228.80	868.85	4288.03	1466.39	1125.61	425.99	781.23	157.11	231.44	1843.49	214.76	165.44	94.30	1131.80	1325.26	109.08	143.13	69.79	206.26	69.68	60.13	63.08	89.38	365.93	90.79	78.92	64.68	4124.62	4864.90	10040.03	5640.95	2586.09	4839.49	11120.87	3644.17	4133.14	1742.97	12921.74	8149.03	1248.10	1094.87
ATP	4462.56	1617.21	416.06	2326.73	1076.46	442.57	629.87	1595.01	6917.69	2229.26	2811.10	4746.44	2463.69	353.09	0.00	174.14	355.67	199.79	404.81	230.96	626.81	238.20	99.81	73.11	254.52	484.64	227.81	0.00	145.63	143.58	0.00	139.97	121.98	93.74	143.57	0.00	182.33	0.00	11066.04	0.00	4408.58	5132.99	201.16	260.72	590.63	107.92	5141.16	2533.61	1577.94	467.35	1282.26	2703.69
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	PubChem CID
Adenosine	60961
AMP	6083
ADP	6022
ATP	5957
METABOLITES_END
#END