#METABOLOMICS WORKBENCH araskind_20160413_085210 DATATRACK_ID:582 STUDY_ID:ST000371 ANALYSIS_ID:AN000605 PROJECT_ID:PR000294 VERSION 1 CREATED_ON April 18, 2016, 5:27 pm #PROJECT PR:PROJECT_TITLE Bacterial Regulators of Tumorigenesis PR:PROJECT_SUMMARY identify bacterial products associated with reduced tumorigenesis PR:INSTITUTE University of Michigan PR:DEPARTMENT Surgery PR:LABORATORY Lubman, David Lab PR:LAST_NAME Lubman PR:FIRST_NAME David PR:ADDRESS Ann Arbor, MI PR:EMAIL dmlubman@umich.edu PR:PHONE 734-647-8834 #STUDY ST:STUDY_TITLE Colon cancer ST:STUDY_TYPE Regular ST:STUDY_SUMMARY There are 4 germfree controls and 4 germfree with bacteria. After treatment, the ST:STUDY_SUMMARY mouse stool samples were collected at different timepoints: day 0, day 10, day ST:STUDY_SUMMARY 26, day 47, and day 61 or 70. Place the stool samples into liquid nitrogen after ST:STUDY_SUMMARY they were collected, then stored in -80oC. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 11 ST:TOTAL_SUBJECTS 41 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0012170 S00018140 Time (day):- | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012170 S00018141 Time (day):10 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012170 S00018142 Time (day):26 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012170 S00018143 Time (day):47 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012170 S00018144 Time (day):61 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012171 S00018145 Time (day):- | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012171 S00018146 Time (day):10 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012171 S00018147 Time (day):26 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012171 S00018148 Time (day):47 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012171 S00018149 Time (day):61 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012172 S00018150 Time (day):- | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012173 S00018151 Time (day):10 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012173 S00018152 Time (day):26 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012173 S00018153 Time (day):47 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012173 S00018154 Time (day):61 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012174 S00018155 Time (day):- | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012174 S00018156 Time (day):10 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012174 S00018157 Time (day):26 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012174 S00018158 Time (day):47 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012174 S00018159 Time (day):61 | Treatment:GF bacteria SUBJECT_SAMPLE_FACTORS SU0012175 S00018160 Time (day):- | Treatment:none SUBJECT_SAMPLE_FACTORS SU0012176 S00018161 Time (day):10 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012176 S00018162 Time (day):26 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012176 S00018163 Time (day):47 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012176 S00018164 Time (day):70 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012175 S00018165 Time (day):- | Treatment:none SUBJECT_SAMPLE_FACTORS SU0012177 S00018166 Time (day):10 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012177 S00018167 Time (day):26 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012177 S00018168 Time (day):47 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012177 S00018169 Time (day):70 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012175 S00018170 Time (day):- | Treatment:none SUBJECT_SAMPLE_FACTORS SU0012178 S00018171 Time (day):10 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012178 S00018172 Time (day):26 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012178 S00018173 Time (day):47 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012178 S00018174 Time (day):70 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012179 S00018175 Time (day):- | Treatment:none SUBJECT_SAMPLE_FACTORS SU0012180 S00018176 Time (day):10 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012180 S00018177 Time (day):26 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012180 S00018178 Time (day):47 | Treatment:GF no bacteria SUBJECT_SAMPLE_FACTORS SU0012180 S00018179 Time (day):70 | Treatment:GF no bacteria #COLLECTION CO:COLLECTION_SUMMARY None CO:SAMPLE_TYPE Pooled sample #TREATMENT TR:TREATMENT_SUMMARY none #SAMPLEPREP SP:SAMPLEPREP_SUMMARY none SP:SAMPLEPREP_PROTOCOL_FILENAME ShortChainFattyAcids-20150305.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890A CH:COLUMN_NAME Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um) CH:COLUMN_TEMPERATURE WAXPLUS_100-200C_2_SIM.M.zip #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975C MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg sample MS_METABOLITE_DATA_START Samples S00018140 S00018141 S00018142 S00018143 S00018144 S00018145 S00018146 S00018147 S00018148 S00018149 S00018150 S00018151 S00018152 S00018153 S00018154 S00018155 S00018156 S00018157 S00018158 S00018159 S00018160 S00018161 S00018162 S00018163 S00018164 S00018165 S00018166 S00018167 S00018168 S00018169 S00018170 S00018171 S00018172 S00018173 S00018174 S00018175 S00018176 S00018177 S00018178 S00018179 Factors Time (day):- | Treatment:GF bacteria Time (day):10 | Treatment:GF bacteria Time (day):26 | Treatment:GF bacteria Time (day):47 | Treatment:GF bacteria Time (day):61 | Treatment:GF bacteria Time (day):- | Treatment:GF bacteria Time (day):10 | Treatment:GF bacteria Time (day):26 | Treatment:GF bacteria Time (day):47 | Treatment:GF bacteria Time (day):61 | Treatment:GF bacteria Time (day):- | Treatment:GF bacteria Time (day):10 | Treatment:GF bacteria Time (day):26 | Treatment:GF bacteria Time (day):47 | Treatment:GF bacteria Time (day):61 | Treatment:GF bacteria Time (day):- | Treatment:GF bacteria Time (day):10 | Treatment:GF bacteria Time (day):26 | Treatment:GF bacteria Time (day):47 | Treatment:GF bacteria Time (day):61 | Treatment:GF bacteria Time (day):- | Treatment:none Time (day):10 | Treatment:GF no bacteria Time (day):26 | Treatment:GF no bacteria Time (day):47 | Treatment:GF no bacteria Time (day):70 | Treatment:GF no bacteria Time (day):- | Treatment:none Time (day):10 | Treatment:GF no bacteria Time (day):26 | Treatment:GF no bacteria Time (day):47 | Treatment:GF no bacteria Time (day):70 | Treatment:GF no bacteria Time (day):- | Treatment:none Time (day):10 | Treatment:GF no bacteria Time (day):26 | Treatment:GF no bacteria Time (day):47 | Treatment:GF no bacteria Time (day):70 | Treatment:GF no bacteria Time (day):- | Treatment:none Time (day):10 | Treatment:GF no bacteria Time (day):26 | Treatment:GF no bacteria Time (day):47 | Treatment:GF no bacteria Time (day):70 | Treatment:GF no bacteria acetic acid 5.67 3.93 4.72 4.71 6.57 4.55 3.88 3.40 3.46 5.68 5.26 3.90 4.33 4.04 4.90 5.24 3.37 4.19 4.53 6.11 1.87 2.07 2.21 1.90 1.96 1.98 1.92 1.85 1.93 1.85 1.73 1.84 2.00 2.07 1.85 1.80 1.80 1.80 1.92 1.73 propionic acid 7.51 5.83 6.70 6.42 8.31 6.21 5.97 5.69 5.79 7.39 7.00 6.05 6.44 6.37 6.62 6.97 5.96 6.62 6.79 8.15 4.47 4.65 4.79 4.60 4.55 4.76 4.81 4.63 4.66 4.58 4.49 4.52 4.67 4.90 4.63 4.55 4.61 4.64 4.64 4.48 iso-butyric acid 4.56 4.49 4.46 4.57 4.57 4.33 4.52 4.60 4.44 4.58 4.62 4.75 4.93 4.62 4.89 4.47 4.67 4.89 4.64 4.67 4.07 4.11 4.10 4.09 4.08 4.10 4.09 4.08 4.07 4.07 4.07 4.10 4.09 4.07 4.08 4.07 4.07 butyric acid 2.42 2.40 2.38 2.36 2.60 2.34 2.43 2.34 2.39 2.55 2.40 2.41 2.36 2.47 2.44 2.42 2.36 2.35 2.73 2.67 2.25 2.26 2.27 2.25 2.26 2.26 2.26 2.25 2.26 2.26 2.25 2.21 2.27 2.26 2.26 2.25 2.25 2.25 2.26 2.25 isovaleric acid 2.42 2.40 2.38 2.36 2.60 2.34 2.43 2.34 2.39 2.55 2.40 2.41 2.36 2.47 2.44 2.42 2.36 2.35 2.73 2.67 2.25 2.26 2.27 2.25 2.26 2.26 2.26 2.25 2.26 2.26 2.25 2.21 2.27 2.26 2.26 2.25 2.25 2.25 2.26 2.25 valeric acid 2.05 2.01 2.03 2.03 2.03 2.00 1.99 2.01 1.99 2.03 2.03 2.02 2.08 2.01 2.05 2.05 2.02 2.04 2.00 2.03 1.93 1.93 1.93 1.93 1.93 1.93 1.93 1.93 1.94 1.93 1.93 1.93 1.93 1.94 1.93 1.93 1.93 1.93 1.95 1.93 hexanoic acid 3.00 3.00 3.00 3.01 3.01 3.00 3.00 3.00 3.00 3.01 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.01 3.02 2.99 2.99 3.00 3.00 2.99 3.00 3.00 2.99 2.99 2.99 2.99 2.99 3.00 2.99 2.99 2.99 2.99 2.99 2.99 2.99 heptanoic acid 3.09 3.15 3.19 3.24 3.14 3.13 3.12 3.12 3.15 3.10 3.15 3.11 3.11 3.13 3.11 3.13 3.13 3.10 3.13 3.10 3.11 3.18 3.14 3.10 3.14 3.11 3.11 3.09 3.10 3.11 3.11 3.11 3.12 3.12 3.10 3.13 3.14 3.14 3.11 3.12 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID acetic acid 176 propionic acid 1032 iso-butyric acid 6590 butyric acid 20975673 isovaleric acid 10430 valeric acid 7991 hexanoic acid 8892 heptanoic acid 8094 METABOLITES_END #END