#METABOLOMICS WORKBENCH araskind_20160413_113726 DATATRACK_ID:584 STUDY_ID:ST000373 ANALYSIS_ID:AN000607 PROJECT_ID:PR000134 VERSION 1 CREATED_ON April 18, 2016, 8:39 pm #PROJECT PR:PROJECT_TITLE Fly Brain Studies PR:PROJECT_SUMMARY Measure change in metabolites according to diet and feeding state PR:INSTITUTE University of Michigan PR:DEPARTMENT MCDB LS&A PR:LABORATORY Dus Lab PR:LAST_NAME Dus PR:FIRST_NAME Monica PR:ADDRESS Ann Arbor, MI PR:EMAIL mdus@umich.edu PR:PHONE 347-891-2287 #STUDY ST:STUDY_TITLE Measure change in metabolites based on diet and feeding status (part II) ST:STUDY_TYPE Regular ST:STUDY_SUMMARY Flies were fed a normal diet or high-sugar (30% sucrose) diet for 10 days. Flies ST:STUDY_SUMMARY of each diet were flash frozen in liquid nitrogen as sated (refed for 3hrs on ST:STUDY_SUMMARY 400mM D-glucose) or after fasting for 24hrs. Heads and bodies were separated ST:STUDY_SUMMARY using a sieve-system and placed immediately on dry ice (total time app. 1min). ST:STUDY_SUMMARY We collected 50 heads per condition (4 conditions total) and 5 biological ST:STUDY_SUMMARY replicates ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core ST:LABORATORY Facilities Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS Ann Arbor, MI ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 18 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Drosophila melanogaster SU:TAXONOMY_ID 7227 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0012355 S00018443 State:sated | Diet:high sugar SUBJECT_SAMPLE_FACTORS SU0012355 S00018444 State:sated | Diet:high sugar SUBJECT_SAMPLE_FACTORS SU0012355 S00018445 State:sated | Diet:high sugar SUBJECT_SAMPLE_FACTORS SU0012355 S00018446 State:sated | Diet:high sugar SUBJECT_SAMPLE_FACTORS SU0012355 S00018447 State:sated | Diet:high sugar SUBJECT_SAMPLE_FACTORS SU0012356 S00018448 State:sated | Diet:normal SUBJECT_SAMPLE_FACTORS SU0012356 S00018449 State:sated | Diet:normal SUBJECT_SAMPLE_FACTORS SU0012356 S00018450 State:sated | Diet:normal SUBJECT_SAMPLE_FACTORS SU0012356 S00018451 State:sated | Diet:normal SUBJECT_SAMPLE_FACTORS SU0012357 S00018452 State:starved | Diet:normal SUBJECT_SAMPLE_FACTORS SU0012357 S00018453 State:starved | Diet:normal SUBJECT_SAMPLE_FACTORS SU0012357 S00018454 State:starved | Diet:normal SUBJECT_SAMPLE_FACTORS SU0012357 S00018455 State:starved | Diet:normal SUBJECT_SAMPLE_FACTORS SU0012357 S00018456 State:starved | Diet:normal SUBJECT_SAMPLE_FACTORS SU0012358 S00018457 State:starved | Diet:high sugar SUBJECT_SAMPLE_FACTORS SU0012358 S00018458 State:starved | Diet:high sugar SUBJECT_SAMPLE_FACTORS SU0012358 S00018459 State:starved | Diet:high sugar SUBJECT_SAMPLE_FACTORS SU0012358 S00018460 State:starved | Diet:high sugar #COLLECTION CO:COLLECTION_SUMMARY NONE CO:SAMPLE_TYPE Tissues #TREATMENT TR:TREATMENT_SUMMARY None #SAMPLEPREP SP:SAMPLEPREP_SUMMARY none SP:SAMPLEPREP_PROTOCOL_FILENAME Glycolysis-TCA-nucleotides-20150324.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE None CH:INSTRUMENT_NAME Unspecified CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um) CH:METHODS_FILENAME GlyTCA-ESN-HILIC-6490.m.zip CH:METHODS_ID DQM0108 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6410A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/µg tissue protein MS_METABOLITE_DATA_START Samples S00018443 S00018444 S00018445 S00018446 S00018447 S00018457 S00018458 S00018459 S00018460 S00018448 S00018449 S00018450 S00018451 S00018452 S00018453 S00018454 S00018455 S00018456 Factors State:sated | Diet:high sugar State:sated | Diet:high sugar State:sated | Diet:high sugar State:sated | Diet:high sugar State:sated | Diet:high sugar State:starved | Diet:high sugar State:starved | Diet:high sugar State:starved | Diet:high sugar State:starved | Diet:high sugar State:sated | Diet:normal State:sated | Diet:normal State:sated | Diet:normal State:sated | Diet:normal State:starved | Diet:normal State:starved | Diet:normal State:starved | Diet:normal State:starved | Diet:normal State:starved | Diet:normal 2 or 3-phosphoglycerate 11.70 7.74 17.53 7.92 8.44 1.52 1.45 1.47 1.81 1.09 1.05 2.10 1.01 1.99 2.44 1.09 0.95 3.67 6-phosphogluconate 10.86 3.61 4.55 3.29 4.09 9.42 5.86 9.16 8.04 3.25 4.72 4.82 4.01 4.80 3.18 4.31 3.38 3.56 acetyl-coa adenosine diphosphate 25.60 11.98 20.48 12.92 11.97 19.18 15.86 18.13 17.70 11.41 14.16 12.06 14.56 15.19 13.76 13.93 10.25 15.69 adenosine monophosphate 78.40 66.30 162.39 71.34 61.25 130.49 136.52 113.90 72.97 86.41 140.52 98.36 158.34 99.36 96.04 160.87 74.79 109.91 adenosine triphosphate 9.78 3.15 5.01 3.47 3.90 6.95 5.00 6.75 6.88 3.04 3.74 3.69 3.39 4.78 3.68 3.91 2.84 4.49 citrate or isocitrate 84.84 80.94 133.93 75.66 64.21 61.54 49.28 52.96 54.03 27.76 49.43 25.70 60.47 17.21 15.77 57.82 16.53 15.82 erythrose 4-phosphate 67.37 54.65 124.40 42.91 55.94 13.01 24.59 15.21 20.83 27.68 32.30 12.52 43.88 91.62 54.75 48.84 44.55 59.06 glucose 6-phosphate or fructose-6-phosphate 73.75 46.75 92.21 32.61 36.04 7.70 5.50 4.06 10.05 9.58 5.62 5.32 11.44 14.44 15.74 8.24 6.85 24.43 flavin adenine dinucleotide 3.09 1.40 2.24 1.24 1.29 2.98 1.97 2.66 2.41 1.41 1.71 1.68 2.42 2.33 1.95 2.20 1.74 1.81 fructose 1,6-bisphosphate 15.85 5.30 7.23 5.03 6.12 12.55 7.87 12.23 10.76 4.37 6.34 6.48 5.36 6.53 4.38 5.82 4.58 5.09 GDP 14.12 4.98 6.48 4.60 5.64 12.21 7.81 11.81 10.63 4.52 6.30 6.41 5.43 6.44 4.44 5.86 4.54 5.11 GMP 23.37 12.99 19.58 10.69 11.56 21.95 15.61 16.67 16.96 14.11 16.12 17.84 20.04 16.97 15.43 15.08 13.05 15.93 GTP 3.34 4.33 3.14 4.20 8.88 5.83 8.53 7.44 3.01 4.55 4.48 3.72 4.45 2.95 4.25 3.22 3.51 malate 125.31 76.25 67.68 46.33 56.45 35.84 10.99 11.60 8.48 23.66 16.95 9.56 31.86 7.36 7.19 8.72 21.02 5.71 nicotinamide adenine dinucleotide oxidized 20.75 12.74 20.52 11.52 13.25 19.03 12.37 14.95 14.25 18.14 20.99 17.46 27.10 18.83 19.07 16.96 15.59 18.56 nicotinamide adenine dinucleotide reduced 8.21 12.20 21.96 10.94 13.86 20.93 12.28 17.36 13.88 19.93 21.77 18.97 28.83 20.36 20.64 18.16 16.12 21.71 nicotinamide adenine dinucleotide phosphate oxidized 8.33 3.00 4.01 2.75 3.37 7.41 4.71 6.95 6.21 3.58 4.33 4.25 4.32 4.51 3.28 3.72 3.16 3.55 nicotinamide adenine dinucleotide phosphate reduced 10.53 3.86 3.56 3.36 3.12 9.30 4.50 8.64 6.36 8.06 3.86 3.82 5.01 3.72 2.34 5.19 3.21 3.12 phosphoenolpyruvate 2.14 0.80 1.76 0.91 1.02 1.77 1.33 1.57 1.62 0.87 1.10 1.02 1.23 1.17 0.93 0.95 0.79 1.06 ribose-5-phosphate or xylulose 5-phosphate 5.85 2.56 4.68 2.08 2.68 1.12 0.71 0.78 0.76 0.93 0.88 0.69 1.28 1.19 1.23 1.07 0.69 1.54 sedoheptulose 7-phosphate 9.15 8.46 12.24 4.15 6.08 3.46 2.29 2.95 3.02 3.68 1.89 2.44 4.35 3.29 2.85 2.13 1.69 3.94 sucrose 193.50 187.88 233.88 100.90 154.28 127.82 27.98 35.10 40.31 121.69 147.75 56.76 313.32 193.99 205.87 138.04 239.80 194.91 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID 2 or 3-phosphoglycerate 724 6-phosphogluconate 91493 acetyl-coa 444493 adenosine diphosphate 6022 adenosine monophosphate 6083 adenosine triphosphate 5957 citrate or isocitrate 311 erythrose 4-phosphate 122357 flavin adenine dinucleotide 46906035 fructose 1,6-bisphosphate 21125049 glucose 6-phosphate or fructose-6-phosphate 5958 malate 525 nicotinamide adenine dinucleotide oxidized 15938971 nicotinamide adenine dinucleotide reduced 439153 nicotinamide adenine dinucleotide phosphate oxidized 15938972 nicotinamide adenine dinucleotide phosphate reduced 22833512 phosphoenolpyruvate 1005 ribose-5-phosphate or xylulose 5-phosphate 77982 sedoheptulose 7-phosphate 165007 sucrose 5988 GDP GMP GTP METABOLITES_END #END