#METABOLOMICS WORKBENCH hormel101_20170623_111514 DATATRACK_ID:1082 STUDY_ID:ST000634 ANALYSIS_ID:AN000966 PROJECT_ID:PR000457 VERSION 1 CREATED_ON June 25, 2017, 9:13 am #PROJECT PR:PROJECT_TITLE Metabolomic Mechanisms of Dietary Salt Effects on Blood Pressure PR:PROJECT_SUMMARY "Enhanced sensitivity of blood pressure to salt intake is observed in PR:PROJECT_SUMMARY approximately 50% of hypertensive patients, reaching 75% in African American PR:PROJECT_SUMMARY hypertensive patients. We recently discovered a novel role of abnormal cellular PR:PROJECT_SUMMARY intermediary metabolism in hypertension in the Dahl salt-sensitive (SS) rat, the PR:PROJECT_SUMMARY most commonly used polygenic, hereditary model of human salt-sensitive PR:PROJECT_SUMMARY hypertension. We propose to test the hypothesis that blood pressure sensitivity PR:PROJECT_SUMMARY to dietary salt intake in human is associated with metabolite changes in the PR:PROJECT_SUMMARY urine. Leveraging the expertise and resources at the Mayo Clinic Metabolomics PR:PROJECT_SUMMARY Resources Core, we propose to perform targeted LC/MS analysis and NMR spectra PR:PROJECT_SUMMARY generation in urine samples obtained from a subset of subjects from the Dietary PR:PROJECT_SUMMARY Approaches to Stop Hypertension – Sodium (DASH2) clinical trial and kidney PR:PROJECT_SUMMARY tissue extract and urine samples from SS rats and a newly generated transgenic PR:PROJECT_SUMMARY rat that overexpresses fumarase (SS.Fh1+). The study will be the first to PR:PROJECT_SUMMARY systematically characterize urinary metabolite profiles associated with blood PR:PROJECT_SUMMARY pressure response to salt in humans. The study is anticipated to generate new PR:PROJECT_SUMMARY insight into the mechanisms (particularly renal mechanisms) underlying PR:PROJECT_SUMMARY salt-sensitive hypertension. Findings of the proposed study could lead to an PR:PROJECT_SUMMARY expanded clinical study as well as mechanistic studies in animal models." PR:INSTITUTE Mayo Clinic PR:LAST_NAME Liang PR:FIRST_NAME Mingyu PR:ADDRESS Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226 PR:EMAIL mliang@mcw.edu PR:PHONE 414-955-8539 #STUDY ST:STUDY_TITLE TCA Cycle Metabolites of Dietary Salt Effects on Blood Pressure in Human Urine ST:STUDY_TITLE from DASH2 Clinical Trial (part VII) ST:STUDY_SUMMARY The objective of the study is to identify changes of urinary metabolite profiles ST:STUDY_SUMMARY associated with different responses to blood pressure to salt. Subjects are ST:STUDY_SUMMARY derived from The Dietary approaches to stop hypertension (DASH) diet, Sodium ST:STUDY_SUMMARY Intake and Blood Pressure Trial (Sacks FM et al PMID: 11136953,N Engl J Med. ST:STUDY_SUMMARY 2001).We choose two groups subjects who meet the following conditions(the two ST:STUDY_SUMMARY groups are separately named A and B). We chose subjects on the Control diet ST:STUDY_SUMMARY .These subjects meet the blood pressure criteria described below:Group A ST:STUDY_SUMMARY subjects conditions: 1) On Control diet. 2) Normotensive subjects: systolic ST:STUDY_SUMMARY blood pressure from the low sodium visit is less than 140 and the diastolic ST:STUDY_SUMMARY blood pressure from low sodium visit is less than 90; 3) For group A: Either the ST:STUDY_SUMMARY systolic blood pressure from the high sodium visit was greater than 10 mmHg ST:STUDY_SUMMARY higher than the systolic blood pressure from the low sodium visit, or the ST:STUDY_SUMMARY diastolic blood pressure from the high sodium visit was greater than 10 mmHg ST:STUDY_SUMMARY higher than the diastolic blood pressure from the low sodium visit; 3) For group ST:STUDY_SUMMARY B: The systolic blood pressure from the high sodium visit is within 5 mmHg (i.e. ST:STUDY_SUMMARY +/- 5) from the systolic blood pressure from the low sodium visit, and the ST:STUDY_SUMMARY diastolic blood pressure from the high sodium visit is within 5 mmHg from the ST:STUDY_SUMMARY diastolic blood pressure from the low sodium visit. Use gas chromatography/mass ST:STUDY_SUMMARY spectrometry (GC/MS) analysis, and liquid chromatography/mass spectrometry ST:STUDY_SUMMARY (LC/MS)analysis to find the differences of metabolic profiles between the high ST:STUDY_SUMMARY sodium level and the low sodium level, and compare the metabolic profiles of A ST:STUDY_SUMMARY with the metabolic profiles of B at the low and high sodium level. ST:INSTITUTE Mayo Clinic ST:LAST_NAME Liang ST:FIRST_NAME Mingyu ST:ADDRESS Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226 ST:EMAIL mliang@mcw.edu ST:PHONE 414-955-8539 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS DASH200394 Sample #1 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200113 Sample #2 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200214 Sample #3 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200143 Sample #4 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200324 Sample #5 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200373 Sample #6 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200309 Sample #7 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200283 Sample #8 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200222 Sample #9 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200169 Sample #10 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200191 Sample #11 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200149 Sample #12 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200267 Sample #13 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200088 Sample #14 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200230 Sample #15 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200065 Sample #16 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200069 Sample #17 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200084 Sample #18 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200058 Sample #19 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200192 Sample #20 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200057 Sample #21 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200278 Sample #22 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200029 Sample #23 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200119 Sample #24 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200057 Sample #25 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200029 Sample #26 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200037 Sample #27 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200037 Sample #28 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200220 Sample #29 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200271 Sample #30 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200045 Sample #31 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200040 Sample #32 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200128 Sample #33 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200406 Sample # 34 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200398 Sample # 35 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200160 Sample # 36 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200031 Sample # 37 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200407 Sample # 38 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200105 Sample # 39 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200020 Sample # 40 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200046 Sample # 41 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200338 Sample # 42 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200149 Sample # 43 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200264 Sample # 44 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200067 Sample # 45 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200150 Sample # 46 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200084 Sample # 47 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200264 Sample # 48 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200267 Sample # 49 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200150 Sample # 50 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200381 Sample # 51 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200222 Sample # 52 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200087 Sample # 53 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200067 Sample # 54 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200142 Sample # 55 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200119 Sample # 56 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200346 Sample # 57 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200185 Sample # 58 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200371 Sample # 59 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200290 Sample # 60 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200220 Sample # 61 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200071 Sample # 62 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200024 Sample # 63 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200370 Sample # 64 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200250 Sample # 65 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200333 Sample # 66 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200186 Sample # 67 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200128 Sample # 68 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200258 Sample # 69 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200398 Sample # 70 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200406 Sample # 71 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200142 Sample # 72 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200309 Sample # 73 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200407 Sample # 74 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200233 Sample # 75 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200158 Sample # 76 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200339 Sample # 77 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200020 Sample # 78 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200381 Sample # 79 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200046 Sample # 80 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200346 Sample # 81 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200371 Sample # 82 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200087 Sample # 83 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200185 Sample # 84 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200043 Sample # 85 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200245 Sample # 86 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200186 Sample # 87 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200043 Sample # 88 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200040 Sample # 89 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200088 Sample # 90 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200058 Sample # 91 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200233 Sample # 92 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200250 Sample # 93 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200192 Sample # 94 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200252 Sample # 95 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200045 Sample # 96 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200283 Sample # 97 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200353 Sample # 98 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200230 Sample # 99 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200386 Sample # 100 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200368 Sample # 101 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200227 Sample # 102 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200249 Sample # 103 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200069 Sample # 104 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200334 Sample # 105 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200198 Sample # 106 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200334 Sample # 107 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200252 Sample # 108 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200261 Sample # 109 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200174 Sample # 110 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200065 Sample # 111 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200245 Sample # 112 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200214 Sample # 113 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200031 Sample # 114 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200368 Sample # 115 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200386 Sample # 116 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200237 Sample # 117 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200227 Sample # 118 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200237 Sample # 119 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200249 Sample # 120 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200198 Sample # 121 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200353 Sample # 122 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200174 Sample # 123 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200373 Sample # 124 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200324 Sample # 125 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200142 Sample # 126 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200105 Sample # 127 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200113 Sample # 128 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200158 Sample # 129 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200394 Sample # 130 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200333 Sample # 131 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200338 Sample # 132 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200261 Sample # 133 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200278 Sample # 134 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200258 Sample # 135 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200271 Sample # 136 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200290 Sample # 137 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200169 Sample # 138 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200191 Sample # 139 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200071 Sample # 140 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200370 Sample # 141 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200024 Sample # 142 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200143 Sample # 143 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200339 Sample # 144 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200042 Sample # 145 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200077 Sample # 146 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200085 Sample # 147 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200179 Sample # 148 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200325 Sample # 149 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200403 Sample # 150 Soduim level:L | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200013 Sample # 151 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200171 Sample # 152 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200276 Sample # 153 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200282 Sample # 154 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200409 Sample # 155 Soduim level:L | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200042 Sample # 156 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200077 Sample # 157 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200085 Sample # 158 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200179 Sample # 159 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200325 Sample # 160 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200403 Sample # 161 Soduim level:H | grouping:A sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200013 Sample # 162 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200171 Sample # 163 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200276 Sample # 164 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200282 Sample # 165 Soduim level:H | grouping:B sample type=human urine SUBJECT_SAMPLE_FACTORS DASH200409 Sample # 166 Soduim level:H | grouping:B sample type=human urine #COLLECTION CO:COLLECTION_SUMMARY The urine samples we have were 24-hour collections occurring in the fourth week CO:COLLECTION_SUMMARY on either the low- or the high-sodium intake level. In total, 120 human samples CO:COLLECTION_SUMMARY (60 subjects, a low-sodium sample and a high-sodium sample for each subject) CO:COLLECTION_SUMMARY will be analyzed. #TREATMENT TR:TREATMENT_SUMMARY We will analyze urine specimens collected during the Dietary Approaches to Stop TR:TREATMENT_SUMMARY Hypertension – Sodium Study (DASH-Sodium or DASH2) clinical trial. More than TR:TREATMENT_SUMMARY 400 participants in the study were divided into two groups. One followed a TR:TREATMENT_SUMMARY common Western diet, while the other group followed the “healthier” DASH TR:TREATMENT_SUMMARY diet. Then each group had three 30 day periods, in random orders, that TR:TREATMENT_SUMMARY corresponded to high, intermediate, or low sodium intakes while eating their TR:TREATMENT_SUMMARY designated diet. The participants had all of their meals prepared and provided TR:TREATMENT_SUMMARY for them during the entire study. It was found that reducing sodium intake TR:TREATMENT_SUMMARY significantly reduced blood pressure especially when on the common Western diet. TR:TREATMENT_SUMMARY We have obtained urine samples and phenotypic data for a subset of the TR:TREATMENT_SUMMARY participants of the DASH2 trial form the NHLBI biospecimen and data repository TR:TREATMENT_SUMMARY BioLINCC. We selected two sub-groups of participants on the Western diet. Group TR:TREATMENT_SUMMARY A (n=31) exhibited large (>12 mmHg) differences in systolic or diastolic TR:TREATMENT_SUMMARY pressure between low- and high-sodium intakes (“salt-sensitive” group). TR:TREATMENT_SUMMARY Group B (n=29) showed similar blood pressure (differences <5 mmHg for both TR:TREATMENT_SUMMARY systolic and diastolic pressures) between low- and high-sodium intakes TR:TREATMENT_SUMMARY (“saltinsensitive” group). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY 2 drops of 6 N HCl was added in every 8 ml urine samples when the 24 hour urine SP:SAMPLEPREP_SUMMARY was collected. All urine samples were stored in -80℃ freezer. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890B CH:COLUMN_NAME Agilent HP5-MS (30m × 0.25mm, 0.25 um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975C MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples Sample #1 Sample #2 Sample #3 Sample #4 Sample #5 Sample #6 Sample #7 Sample #8 Sample #9 Sample #10 Sample #11 Sample #12 Sample #13 Sample #14 Sample #15 Sample #16 Sample #17 Sample #18 Sample #19 Sample #20 Sample #21 Sample #22 Sample #23 Sample #24 Sample #25 Sample #26 Sample #27 Sample #28 Sample #29 Sample #30 Sample #31 Sample #32 Sample #33 Sample # 34 Sample # 35 Sample # 36 Sample # 37 Sample # 38 Sample # 39 Sample # 40 Sample # 41 Sample # 42 Sample # 43 Sample # 44 Sample # 45 Sample # 46 Sample # 47 Sample # 48 Sample # 49 Sample # 50 Sample # 51 Sample # 52 Sample # 53 Sample # 54 Sample # 55 Sample # 56 Sample # 57 Sample # 58 Sample # 59 Sample # 60 Sample # 61 Sample # 62 Sample # 63 Sample # 64 Sample # 65 Sample # 66 Sample # 67 Sample # 68 Sample # 69 Sample # 70 Sample # 71 Sample # 72 Sample # 73 Sample # 74 Sample # 75 Sample # 76 Sample # 77 Sample # 78 Sample # 79 Sample # 80 Sample # 81 Sample # 82 Sample # 83 Sample # 84 Sample # 85 Sample # 86 Sample # 87 Sample # 88 Sample # 89 Sample # 90 Sample # 91 Sample # 92 Sample # 93 Sample # 94 Sample # 95 Sample # 96 Sample # 97 Sample # 98 Sample # 99 Sample # 100 Sample # 101 Sample # 102 Sample # 103 Sample # 104 Sample # 105 Sample # 106 Sample # 107 Sample # 108 Sample # 109 Sample # 110 Sample # 111 Sample # 112 Sample # 113 Sample # 114 Sample # 115 Sample # 116 Sample # 117 Sample # 118 Sample # 119 Sample # 120 Sample # 121 Sample # 122 Sample # 123 Sample # 124 Sample # 125 Sample # 126 Sample # 127 Sample # 128 Sample # 129 Sample # 130 Sample # 131 Sample # 132 Sample # 133 Sample # 134 Sample # 135 Sample # 136 Sample # 137 Sample # 138 Sample # 139 Sample # 140 Sample # 141 Sample # 142 Sample # 143 Sample # 144 Sample # 145 Sample # 146 Sample # 147 Sample # 148 Sample # 149 Sample # 150 Sample # 151 Sample # 152 Sample # 153 Sample # 154 Sample # 155 Sample # 156 Sample # 157 Sample # 158 Sample # 159 Sample # 160 Sample # 161 Sample # 162 Sample # 163 Sample # 164 Sample # 165 Sample # 166 Factors Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:L | grouping:B Soduim level:H | grouping:A Soduim level:L | grouping:B Soduim level:H | grouping:B Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:H | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:B Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:A Soduim level:L | grouping:B Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:L | grouping:B Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:H | grouping:B Soduim level:H | grouping:A Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:B Soduim level:H | grouping:B Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:H | grouping:A Soduim level:L | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:L | grouping:B Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:H | grouping:A Soduim level:L | grouping:B Soduim level:H | grouping:B Soduim level:L | grouping:B Soduim level:H | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:H | grouping:A Soduim level:L | grouping:A Soduim level:H | grouping:B Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:L | grouping:B Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:L | grouping:A Soduim level:L | grouping:B Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:L | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:H | grouping:A Soduim level:L | grouping:A Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:H | grouping:A Soduim level:L | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:L | grouping:B Soduim level:H | grouping:B Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:B Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:H | grouping:B Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:A Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:L | grouping:B Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:A Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Soduim level:H | grouping:B Lactate 84.4 68.2 487.6 848.8 201.4 26.9 84.1 139.2 211.6 123.0 39.3 94.4 80.6 22.4 115.0 856.3 148.3 75.0 153.3 107.6 797.9 596.1 121.6 2365.1 194.7 91.6 39.7 50.3 102.6 58.2 132.7 221.3 209.1 23.7 280.6 739.3 37.0 33.9 416.3 78.5 70.7 29.6 106.5 93.5 255.7 157.4 54.5 67.6 94.6 96.4 66.8 479.2 130.3 182.0 92.1 1090.2 113.4 80.3 437.4 74.0 159.5 252.5 156.4 55.3 344.4 85.7 44.4 49.1 461.5 145.8 20.7 360.6 51.8 49.9 101.8 359.0 40.5 3724.6 50.7 49.3 72.4 206.9 50.1 161.9 55.6 362.9 60.0 45.4 122.9 20.6 154.4 64.1 235.3 256.4 154.1 53.5 94.3 662.7 272.8 528.3 199.9 84.1 323.1 925.6 103.2 531.7 330.2 132.3 226.5 301.1 466.6 60.6 230.6 38.7 187.3 449.7 92.9 50.6 73.6 441.9 872.0 222.7 607.7 54.9 181.3 186.5 707.5 84.2 312.2 46.6 140.4 28.8 122.6 98.1 643.7 744.7 69.5 151.2 87.2 259.3 116.2 378.6 423.5 29.3 169.5 244.6 138.8 141.4 127.1 172.8 126.2 75.2 134.5 164.6 1216.3 392.4 181.5 225.7 232.2 161.9 257.4 51.2 181.5 164.9 80.1 1634.1 Succinate 15.5 13.2 227.7 368.7 36.6 4.6 21.1 14.2 26.9 24.4 7.8 24.9 61.0 46.2 13.3 86.0 68.2 13.7 56.0 2.3 58.0 77.9 17.8 141.2 67.7 23.7 9.9 7.2 13.1 10.1 32.3 176.2 33.7 4.6 38.8 36.0 16.5 10.7 10.0 40.4 20.2 6.1 25.4 26.8 118.5 17.0 12.4 25.5 83.3 21.6 7.3 55.0 15.1 170.3 29.5 450.8 14.5 17.1 113.8 15.7 12.1 171.8 4.0 8.7 17.2 11.1 6.1 14.1 167.2 13.5 3.0 19.0 17.1 12.0 741.5 67.5 8.1 36.4 4.6 9.0 14.3 54.0 9.1 49.4 28.8 15.8 4.7 20.8 14.2 19.9 251.8 591.2 72.9 7.3 32.4 9.7 9.1 369.1 36.0 158.3 483.8 8.5 147.8 64.7 7.2 30.6 5.4 21.6 1545.6 82.7 68.0 15.3 61.5 18.4 375.9 574.0 24.9 5.1 19.7 25.8 71.9 62.5 429.3 7.6 27.1 118.3 71.8 22.7 18.1 9.1 5.1 3.6 1564.3 9.7 471.5 29.0 9.8 51.0 10.7 98.1 11.9 9.5 38.4 4.9 21.6 89.7 12.5 14.1 29.9 18.0 13.2 17.6 45.1 34.3 126.2 95.5 109.6 56.4 67.7 270.1 12.8 8.9 32.1 336.4 11.2 136.4 Fumarate 7.2 5.6 4.7 12.2 17.3 2.8 11.2 8.2 13.7 11.4 4.0 13.0 11.9 5.3 6.7 6.5 10.6 6.2 13.1 3.6 11.3 8.6 14.7 19.2 16.1 13.0 6.9 8.6 8.2 4.6 3.1 4.4 6.4 2.8 7.6 22.7 8.3 4.8 11.7 6.8 7.9 5.8 11.1 9.4 20.6 9.4 5.5 7.9 13.0 8.8 4.7 24.0 9.2 22.8 3.1 64.5 15.5 28.1 11.7 8.4 9.7 4.5 3.8 4.0 5.1 4.7 5.4 5.7 3.3 3.7 3.2 19.4 8.2 6.2 2.7 10.3 3.8 23.6 3.4 5.1 8.0 11.4 5.8 299.9 15.8 12.4 4.8 7.0 9.9 5.2 5.1 3.2 3.0 6.3 12.3 4.9 7.6 17.5 21.0 6.9 8.2 6.8 4.8 11.1 5.5 7.0 6.3 8.4 30.0 19.7 14.4 8.8 3.2 13.4 7.6 4.6 8.4 4.3 8.5 11.0 5.3 19.1 27.2 2.5 16.7 4.0 2.7 7.5 7.5 3.8 4.0 2.1 33.2 5.7 4.9 10.8 7.3 13.1 5.6 8.3 6.0 7.6 13.2 3.8 8.9 9.1 6.7 6.9 7.4 7.2 15.4 7.2 20.1 14.9 8.6 18.9 5.0 10.3 18.2 3.5 7.7 6.4 17.8 3.5 9.4 9.0 Oxaloacetate 1.0 2.1 2.1 2.7 1.5 1.0 1.8 2.3 1.9 1.7 0.7 1.4 2.1 0.6 1.5 3.8 1.2 1.4 1.6 0.3 2.2 1.9 3.0 1.6 1.7 2.7 1.1 1.1 1.1 1.0 0.9 2.4 1.1 0.7 0.8 1.0 1.4 0.6 2.8 0.8 1.2 0.7 0.9 0.9 1.1 1.1 1.1 0.9 1.2 1.1 1.0 1.2 1.3 1.2 0.6 0.6 1.3 1.2 1.6 1.4 1.0 1.3 0.9 1.0 1.8 0.9 1.3 1.2 1.2 0.9 1.1 1.5 1.4 1.6 0.9 1.8 0.9 2.1 0.7 1.2 1.9 3.0 1.3 1.5 1.2 1.9 1.1 1.7 1.8 0.7 0.7 1.8 3.1 1.0 1.4 1.0 1.9 2.1 2.6 1.8 0.4 1.8 2.2 1.6 1.7 2.5 1.3 2.0 1.2 1.7 2.9 2.6 2.0 1.1 1.6 5.3 2.2 1.8 2.0 3.1 4.4 3.0 2.7 1.9 2.7 1.5 1.7 3.2 3.4 0.8 2.0 0.7 3.0 2.3 1.7 1.6 1.2 1.1 0.9 1.1 1.1 1.3 1.9 0.7 1.2 0.8 0.8 1.1 4.0 1.5 1.5 1.1 1.1 0.8 3.5 1.2 1.1 1.1 0.9 0.9 0.8 0.8 1.1 1.2 1.0 0.9 Ketoglutarate 116.8 67.1 176.8 116.7 255.1 25.3 124.9 73.6 166.3 130.3 59.7 138.8 139.8 97.6 41.6 120.4 99.6 56.2 351.2 8.9 295.6 91.5 81.3 433.4 210.6 89.5 68.6 64.8 88.5 78.4 98.3 131.5 93.8 18.2 167.1 152.1 91.2 59.2 72.0 39.5 73.8 38.7 144.0 260.5 315.5 140.2 45.7 193.8 136.8 124.6 39.3 211.1 10.2 360.5 23.7 320.7 98.3 104.4 211.2 66.0 107.7 25.4 22.0 40.7 85.2 34.5 14.4 69.5 88.8 67.2 16.1 114.4 95.1 64.1 51.4 94.3 53.9 98.7 22.8 46.2 83.3 158.8 33.3 373.1 115.9 103.9 7.5 78.5 134.2 103.0 98.4 66.0 69.3 40.3 98.1 86.0 56.8 215.0 169.7 140.0 52.5 45.5 155.6 39.0 65.3 197.8 51.8 62.9 65.0 354.5 155.8 90.9 109.9 57.4 61.0 81.5 66.2 36.5 95.5 180.1 158.0 267.0 204.6 18.5 207.6 37.7 65.6 20.8 95.2 79.8 20.3 20.0 65.6 55.6 97.5 98.9 48.8 97.4 66.4 9.9 13.7 17.8 128.0 42.5 115.4 90.1 41.3 24.8 119.8 56.5 88.2 56.7 216.0 120.8 293.4 181.1 65.2 91.4 218.7 5.5 54.5 54.4 165.3 54.5 79.3 424.8 Malate 6.4 5.8 73.2 25.5 18.1 1.0 9.3 6.8 10.9 6.4 4.6 17.1 10.9 4.7 9.3 13.1 19.9 2.8 12.2 5.7 16.8 7.7 8.4 137.8 13.9 7.2 5.3 8.3 9.5 2.4 0.6 16.6 4.2 1.0 5.2 37.2 7.9 2.1 11.2 0.8 5.3 3.3 12.6 7.6 20.2 9.5 2.9 6.6 10.9 9.2 3.0 25.5 14.9 21.8 2.8 268.9 29.3 9.4 14.7 5.0 13.7 1.9 2.8 3.2 11.3 3.0 3.4 3.7 12.4 2.3 0.9 24.7 5.2 2.4 2.0 12.6 2.8 31.3 2.9 4.0 12.8 11.8 3.8 25.4 25.1 15.8 5.1 6.7 8.3 4.5 2.5 1.5 15.8 9.4 7.8 5.3 16.4 75.6 18.5 13.1 9.3 2.5 7.5 31.5 3.1 6.9 4.8 6.1 2.1 23.7 12.5 6.5 11.9 13.4 1.5 90.7 5.2 1.9 4.9 10.8 4.5 22.9 71.1 1.1 15.9 1.1 15.7 10.3 7.2 3.1 2.2 1.3 1.4 4.2 27.7 4.8 3.7 10.2 6.5 26.2 5.2 5.6 6.9 2.3 6.7 8.0 3.9 5.7 15.2 4.4 18.4 5.5 20.0 13.1 29.3 21.4 4.6 13.0 14.1 1.2 2.8 4.3 10.7 3.4 6.9 34.2 2-Hydroxyglutarate 16.2 21.5 35.5 32.0 27.5 2.2 19.2 16.0 13.4 15.1 11.8 42.9 23.5 16.5 29.4 36.5 58.9 9.9 32.9 5.7 36.0 15.2 22.7 51.7 26.1 24.6 15.4 18.1 21.0 10.9 14.8 26.6 15.8 4.8 18.0 24.3 13.7 11.3 9.4 19.9 13.5 11.4 31.3 17.4 36.4 45.0 8.7 20.0 29.9 39.7 10.8 20.2 24.4 44.6 4.2 34.1 30.4 29.7 30.6 13.9 22.8 8.6 8.8 17.6 13.4 12.4 6.1 5.5 4.3 3.1 1.5 4.7 2.9 3.5 3.1 3.4 2.6 6.7 2.0 2.8 7.1 28.0 3.4 8.2 8.4 4.6 2.8 3.2 6.8 3.9 5.1 2.5 3.2 2.6 6.9 3.7 5.6 6.3 43.7 3.9 34.8 8.4 28.3 90.0 9.9 20.9 9.3 18.7 16.1 45.9 30.2 17.0 17.9 12.2 19.5 12.6 16.7 7.2 17.7 29.1 19.7 24.3 48.2 2.6 21.5 8.2 16.2 39.2 13.4 9.8 8.0 5.5 9.2 10.0 36.1 19.9 6.2 16.5 14.6 8.5 20.2 13.2 39.1 7.1 31.3 19.8 6.1 17.7 57.1 29.1 21.5 11.4 33.1 17.2 29.8 46.8 20.1 16.6 42.8 39.4 15.4 11.9 35.0 25.8 12.9 39.2 cis-Aconitic Acid 91.0 108.2 129.0 269.1 157.9 49.5 226.9 143.4 186.0 198.5 43.3 195.0 173.7 84.6 156.2 118.0 134.3 166.9 110.3 59.3 170.0 127.8 348.8 160.0 196.1 322.6 122.6 163.9 119.7 137.7 74.4 126.7 87.6 66.4 141.4 182.8 86.8 60.6 66.1 74.7 139.9 116.6 148.6 84.4 982.1 161.5 103.7 61.7 152.8 141.3 46.5 203.5 102.4 127.2 33.2 105.5 199.2 100.6 189.1 191.9 108.0 154.3 66.2 65.3 86.6 120.7 109.8 104.8 40.2 65.3 95.3 154.8 173.6 128.7 121.2 108.0 51.8 195.4 41.2 119.4 184.5 180.0 96.7 161.7 166.1 177.3 60.2 83.5 115.1 64.9 85.6 143.6 73.2 113.6 193.7 74.0 158.7 181.4 521.4 156.0 110.7 142.6 80.8 171.1 92.7 82.5 89.6 105.8 115.7 232.3 155.0 114.3 72.6 96.2 117.3 90.0 120.0 100.5 123.1 101.8 70.7 151.9 341.8 36.2 118.8 32.7 52.0 68.7 71.9 29.3 82.5 27.1 72.9 69.4 56.7 113.9 129.6 300.1 39.6 175.8 52.6 73.4 305.1 82.6 122.0 118.2 121.1 61.7 584.0 116.0 106.3 123.5 203.9 235.1 163.9 210.8 98.6 80.2 167.8 84.6 77.4 62.8 309.7 95.9 108.4 202.0 Citrate 1509 2308 3003 1864 3313 736 2083 2206 4240 2512 1216 2913 3366 2545 866 3198 777 1147 3750 185 4735 1769 1646 7441 3791 1626 1314 1083 1821 920 1306 2708 1310 441 3244 4042 903 1659 1177 1392 1903 935 3088 3892 6027 2002 1128 3580 3392 1694 1468 6400 1506 6857 435 6073 3299 1629 2484 1924 1938 98 413 1451 2190 1079 728 1150 2285 905 457 2846 1662 1956 4 2411 930 2382 1062 1515 1585 1986 1529 3792 2310 2714 704 1895 3551 2320 1634 8 1567 630 2369 1138 1631 3628 2897 1578 3336 495 2276 134 792 2410 496 1385 1620 4365 3240 1902 1700 745 1368 343 1102 412 1397 3052 2750 4270 1387 638 2856 440 905 2340 2330 1281 660 606 551 1206 3244 1570 1272 1999 1581 136 1743 1133 1362 517 1922 2036 680 1526 2501 984 1559 1369 2546 3453 4127 3234 1308 2156 4448 1031 1092 927 3300 3 1920 5367 Isocitrate 123.0 164.9 195.5 315.5 280.7 67.9 281.5 196.5 294.7 251.4 63.9 268.1 238.1 112.2 210.5 192.9 152.0 231.8 195.9 78.5 310.7 190.2 458.1 249.7 335.0 437.1 168.5 207.8 141.9 184.2 103.4 171.9 113.7 129.3 272.4 344.6 162.3 115.7 116.2 139.5 234.1 176.2 302.0 178.6 247.3 257.8 163.6 152.7 304.2 236.5 85.0 410.5 135.5 271.9 54.1 213.5 303.8 141.9 298.7 278.7 157.0 241.3 114.8 105.4 122.4 259.3 128.5 173.9 80.2 131.4 138.1 256.7 252.8 223.1 1.9 183.8 94.4 269.4 65.6 181.6 271.0 293.3 170.9 266.0 234.5 259.8 71.7 152.2 183.7 117.0 124.3 2.3 129.8 127.3 345.0 126.2 238.2 304.7 720.5 240.7 164.8 223.1 187.3 312.1 167.2 134.1 154.8 167.2 99.7 250.0 257.9 203.5 118.4 154.5 178.7 111.8 221.7 166.3 216.5 218.3 121.2 297.5 418.2 62.1 198.3 65.8 117.5 126.9 131.8 64.2 189.5 59.0 65.2 172.9 113.7 223.4 232.2 321.4 86.7 191.1 115.4 155.9 479.4 96.8 237.8 208.5 181.8 122.0 725.1 186.4 189.1 227.2 381.7 326.5 262.9 380.5 211.6 175.2 302.4 186.7 153.5 143.4 489.6 107.2 208.2 335.9 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Lactate Succinate Fumarate Oxaloacetate Ketoglutarate Malate 2-Hydroxyglutarate cis-Aconitic Acid Citrate Isocitrate METABOLITES_END #END