#METABOLOMICS WORKBENCH hormel101_20170623_113812 DATATRACK_ID:1085 STUDY_ID:ST000636 ANALYSIS_ID:AN000968 PROJECT_ID:PR000457 VERSION 1 CREATED_ON June 25, 2017, 9:23 am #PROJECT PR:PROJECT_TITLE Metabolomic Mechanisms of Dietary Salt Effects on Blood Pressure PR:PROJECT_SUMMARY "Enhanced sensitivity of blood pressure to salt intake is observed in PR:PROJECT_SUMMARY approximately 50% of hypertensive patients, reaching 75% in African American PR:PROJECT_SUMMARY hypertensive patients. We recently discovered a novel role of abnormal cellular PR:PROJECT_SUMMARY intermediary metabolism in hypertension in the Dahl salt-sensitive (SS) rat, the PR:PROJECT_SUMMARY most commonly used polygenic, hereditary model of human salt-sensitive PR:PROJECT_SUMMARY hypertension. We propose to test the hypothesis that blood pressure sensitivity PR:PROJECT_SUMMARY to dietary salt intake in human is associated with metabolite changes in the PR:PROJECT_SUMMARY urine. Leveraging the expertise and resources at the Mayo Clinic Metabolomics PR:PROJECT_SUMMARY Resources Core, we propose to perform targeted LC/MS analysis and NMR spectra PR:PROJECT_SUMMARY generation in urine samples obtained from a subset of subjects from the Dietary PR:PROJECT_SUMMARY Approaches to Stop Hypertension – Sodium (DASH2) clinical trial and kidney PR:PROJECT_SUMMARY tissue extract and urine samples from SS rats and a newly generated transgenic PR:PROJECT_SUMMARY rat that overexpresses fumarase (SS.Fh1+). The study will be the first to PR:PROJECT_SUMMARY systematically characterize urinary metabolite profiles associated with blood PR:PROJECT_SUMMARY pressure response to salt in humans. The study is anticipated to generate new PR:PROJECT_SUMMARY insight into the mechanisms (particularly renal mechanisms) underlying PR:PROJECT_SUMMARY salt-sensitive hypertension. Findings of the proposed study could lead to an PR:PROJECT_SUMMARY expanded clinical study as well as mechanistic studies in animal models." PR:INSTITUTE Mayo Clinic PR:LAST_NAME Liang PR:FIRST_NAME Mingyu PR:ADDRESS Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226 PR:EMAIL mliang@mcw.edu PR:PHONE 414-955-8539 #STUDY ST:STUDY_TITLE Neuromodulator Metabolites of Dietary Salt Effects on Blood Pressure in Human ST:STUDY_TITLE Urine from DASH2 Clinical Trial (part IX) ST:STUDY_SUMMARY The objective of the study is to identify changes of urinary metabolite profiles ST:STUDY_SUMMARY associated with different responses to blood pressure to salt. Subjects are ST:STUDY_SUMMARY derived from The Dietary approaches to stop hypertension (DASH) diet, Sodium ST:STUDY_SUMMARY Intake and Blood Pressure Trial (Sacks FM et al PMID: 11136953,N Engl J Med. ST:STUDY_SUMMARY 2001).We choose two groups subjects who meet the following conditions(the two ST:STUDY_SUMMARY groups are separately named A and B). We chose subjects on the Control diet ST:STUDY_SUMMARY .These subjects meet the blood pressure criteria described below:Group A ST:STUDY_SUMMARY subjects conditions: 1) On Control diet. 2) Normotensive subjects: systolic ST:STUDY_SUMMARY blood pressure from the low sodium visit is less than 140 and the diastolic ST:STUDY_SUMMARY blood pressure from low sodium visit is less than 90; 3) For group A: Either the ST:STUDY_SUMMARY systolic blood pressure from the high sodium visit was greater than 10 mmHg ST:STUDY_SUMMARY higher than the systolic blood pressure from the low sodium visit, or the ST:STUDY_SUMMARY diastolic blood pressure from the high sodium visit was greater than 10 mmHg ST:STUDY_SUMMARY higher than the diastolic blood pressure from the low sodium visit; 3) For group ST:STUDY_SUMMARY B: The systolic blood pressure from the high sodium visit is within 5 mmHg (i.e. ST:STUDY_SUMMARY +/- 5) from the systolic blood pressure from the low sodium visit, and the ST:STUDY_SUMMARY diastolic blood pressure from the high sodium visit is within 5 mmHg from the ST:STUDY_SUMMARY diastolic blood pressure from the low sodium visit. Use gas chromatography/mass ST:STUDY_SUMMARY spectrometry (GC/MS) analysis, and liquid chromatography/mass spectrometry ST:STUDY_SUMMARY (LC/MS)analysis to find the differences of metabolic profiles between the high ST:STUDY_SUMMARY sodium level and the low sodium level, and compare the metabolic profiles of A ST:STUDY_SUMMARY with the metabolic profiles of B at the low and high sodium level. ST:INSTITUTE Mayo Clinic ST:LAST_NAME Liang ST:FIRST_NAME Mingyu ST:ADDRESS Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226 ST:EMAIL mliang@mcw.edu ST:PHONE 414-955-8539 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS DASH200394 ms5957-1 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200113 ms5957-2 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200214 ms5957-3 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200143 ms5957-4 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200324 ms5957-5 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200373 ms5957-6 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200309 ms5957-7 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200283 ms5957-8 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200222 ms5957-9 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200169 ms5957-10 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200191 ms5957-11 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200149 ms5957-12 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200267 ms5957-13 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200088 ms5957-14 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200230 ms5957-15 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200065 ms5957-16 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200069 ms5957-17 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200084 ms5957-18 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200058 ms5957-19 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200192 ms5957-20 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200057 ms5957-21 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200278 ms5957-22 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200029 ms5957-23 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200119 ms5957-24 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200057 ms5957-25 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200029 ms5957-26 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200037 ms5957-27 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200037 ms5957-28 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200220 ms5957-29 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200271 ms5957-30 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200045 ms5957-31 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200040 ms5957-32 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200128 ms5957-33 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200406 ms5957-34 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200398 ms5957-35 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200160 ms5957-36 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200031 ms5957-37 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200407 ms5957-38 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200105 ms5957-39 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200020 ms5957-40 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200046 ms5957-41 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200338 ms5957-42 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200149 ms5957-43 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200264 ms5957-44 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200067 ms5957-45 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200150 ms5957-46 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200084 ms5957-47 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200264 ms5957-48 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200267 ms5957-49 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200150 ms5957-50 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200381 ms5957-51 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200222 ms5957-52 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200087 ms5957-53 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200067 ms5957-54 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200142 ms5957-55 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200119 ms5957-56 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200346 ms5957-57 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200185 ms5957-58 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200371 ms5957-59 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200290 ms5957-60 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200220 ms5957-61 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200071 ms5957-62 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200024 ms5957-63 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200370 ms5957-64 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200250 ms5957-65 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200333 ms5957-66 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200186 ms5957-67 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200128 ms5957-68 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200258 ms5957-69 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200398 ms5957-70 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200406 ms5957-71 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200142 ms5957-72 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200309 ms5957-73 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200407 ms5957-74 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200233 ms5957-75 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200158 ms5957-76 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200339 ms5957-77 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200020 ms5957-78 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200381 ms5957-79 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200046 ms5957-80 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200346 ms5957-81 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200371 ms5957-82 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200087 ms5957-83 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200185 ms5957-84 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200043 ms5957-85 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200245 ms5957-86 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200186 ms5957-87 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200043 ms5957-88 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200040 ms5957-89 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200088 ms5957-90 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200058 ms5957-91 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200233 ms5957-92 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200250 ms5957-93 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200192 ms5957-94 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200252 ms5957-95 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200045 ms5957-96 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200283 ms5957-97 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200353 ms5957-98 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200230 ms5957-99 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200386 ms5957-100 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200368 ms5957101 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200227 ms5957-102 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200249 ms5957-103 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200069 ms5957-104 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200334 ms5957-105 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200198 ms5957-106 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200334 ms5957-107 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200252 ms5957-108 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200261 ms5957-109 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200174 ms5957-110 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200065 ms5957-111 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200245 ms5957-112 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200214 ms5957-113 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200031 ms5957-114 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200368 ms5957-115 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200386 ms5957-116 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200237 ms5957-117 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200227 ms5957-118 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200237 ms5957-119 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200249 ms5957-120 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200198 ms5957-121 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200353 ms5957-122 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200174 ms5957-123 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200373 ms5957-124 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200324 ms5957-125 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200142 ms5957-126 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200105 ms5957-127 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200113 ms5957-128 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200158 ms5957-129 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200394 ms5957-130 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200333 ms5957-131 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200338 ms5957-132 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200261 ms5957-133 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200278 ms5957-134 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200258 ms5957-135 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200271 ms5957-136 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200290 ms5957-137 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200169 ms5957-138 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200191 ms5957-139 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200071 ms5957-140 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200370 ms5957-141 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200024 ms5957-142 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200143 ms5957-143 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200339 ms5957-144 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200042 ms5957-145 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200077 ms5957-146 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200085 ms5957-147 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200179 ms5957-148 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200325 ms5957-149 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200403 ms5957-150 Sodium level:L | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200013 ms5957-151 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200171 ms5957-152 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200276 ms5957-153 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200282 ms5957-154 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200409 ms5957-155 Sodium level:L | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200042 ms5957-156 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200077 ms5957-157 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200085 ms5957-158 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200179 ms5957-159 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200325 ms5957-160 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200403 ms5957-161 Sodium level:H | grouping:A Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200013 ms5957-162 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200171 ms5957-163 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200276 ms5957-164 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200282 ms5957-165 Sodium level:H | grouping:B Sample type=urine; Species=human SUBJECT_SAMPLE_FACTORS DASH200409 ms5957-166 Sodium level:H | grouping:B Sample type=urine; Species=human #COLLECTION CO:COLLECTION_SUMMARY The urine samples we have were 24-hour collections occurring in the fourth week CO:COLLECTION_SUMMARY on either the low- or the high-sodium intake level. In total, 120 human samples CO:COLLECTION_SUMMARY (60 subjects, a low-sodium sample and a high-sodium sample for each subject) CO:COLLECTION_SUMMARY will be analyzed. #TREATMENT TR:TREATMENT_SUMMARY We will analyze urine specimens collected during the Dietary Approaches to Stop TR:TREATMENT_SUMMARY Hypertension – Sodium Study (DASH-Sodium or DASH2) clinical trial. More than TR:TREATMENT_SUMMARY 400 participants in the study were divided into two groups. One followed a TR:TREATMENT_SUMMARY common Western diet, while the other group followed the “healthier” DASH TR:TREATMENT_SUMMARY diet. Then each group had three 30 day periods, in random orders, that TR:TREATMENT_SUMMARY corresponded to high, intermediate, or low sodium intakes while eating their TR:TREATMENT_SUMMARY designated diet. The participants had all of their meals prepared and provided TR:TREATMENT_SUMMARY for them during the entire study. It was found that reducing sodium intake TR:TREATMENT_SUMMARY significantly reduced blood pressure especially when on the common Western diet. TR:TREATMENT_SUMMARY We have obtained urine samples and phenotypic data for a subset of the TR:TREATMENT_SUMMARY participants of the DASH2 trial form the NHLBI biospecimen and data repository TR:TREATMENT_SUMMARY BioLINCC. We selected two sub-groups of participants on the Western diet. Group TR:TREATMENT_SUMMARY A (n=31) exhibited large (>12 mmHg) differences in systolic or diastolic TR:TREATMENT_SUMMARY pressure between low- and high-sodium intakes (“salt-sensitive” group). TR:TREATMENT_SUMMARY Group B (n=29) showed similar blood pressure (differences <5 mmHg for both TR:TREATMENT_SUMMARY systolic and diastolic pressures) between low- and high-sodium intakes TR:TREATMENT_SUMMARY (“saltinsensitive” group). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY 2 drops of 6 N HCl was added in every 8 ml urine samples when the 24 hour urine SP:SAMPLEPREP_SUMMARY was collected. All urine samples were stored in -80℃ freezer. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Waters Acquity BEH C18 (150 x 2.1mm, 1.7um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Thermo Quantum Ultra MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples ms5957-1 ms5957-2 ms5957-3 ms5957-4 ms5957-5 ms5957-6 ms5957-7 ms5957-8 ms5957-9 ms5957-10 ms5957-11 ms5957-12 ms5957-13 ms5957-14 ms5957-15 ms5957-16 ms5957-17 ms5957-18 ms5957-19 ms5957-20 ms5957-21 ms5957-22 ms5957-23 ms5957-24 ms5957-25 ms5957-26 ms5957-27 ms5957-28 ms5957-29 ms5957-30 ms5957-31 ms5957-32 ms5957-33 ms5957-34 ms5957-35 ms5957-36 ms5957-37 ms5957-38 ms5957-39 ms5957-40 ms5957-41 ms5957-42 ms5957-43 ms5957-44 ms5957-45 ms5957-46 ms5957-47 ms5957-48 ms5957-49 ms5957-50 ms5957-51 ms5957-52 ms5957-53 ms5957-54 ms5957-55 ms5957-56 ms5957-57 ms5957-58 ms5957-59 ms5957-60 ms5957-61 ms5957-62 ms5957-63 ms5957-64 ms5957-65 ms5957-66 ms5957-67 ms5957-68 ms5957-69 ms5957-70 ms5957-71 ms5957-72 ms5957-73 ms5957-74 ms5957-75 ms5957-76 ms5957-77 ms5957-78 ms5957-79 ms5957-80 ms5957-81 ms5957-82 ms5957-83 ms5957-84 ms5957-85 ms5957-86 ms5957-87 ms5957-88 ms5957-89 ms5957-90 ms5957-91 ms5957-92 ms5957-93 ms5957-94 ms5957-95 ms5957-96 ms5957-97 ms5957-98 ms5957-99 ms5957-100 ms5957101 ms5957-102 ms5957-103 ms5957-104 ms5957-105 ms5957-106 ms5957-107 ms5957-108 ms5957-109 ms5957-110 ms5957-111 ms5957-112 ms5957-113 ms5957-114 ms5957-115 ms5957-116 ms5957-117 ms5957-118 ms5957-119 ms5957-120 ms5957-121 ms5957-122 ms5957-123 ms5957-124 ms5957-125 ms5957-126 ms5957-127 ms5957-128 ms5957-129 ms5957-130 ms5957-131 ms5957-132 ms5957-133 ms5957-134 ms5957-135 ms5957-136 ms5957-137 ms5957-138 ms5957-139 ms5957-140 ms5957-141 ms5957-142 ms5957-143 ms5957-144 ms5957-145 ms5957-146 ms5957-147 ms5957-148 ms5957-149 ms5957-150 ms5957-151 ms5957-152 ms5957-153 ms5957-154 ms5957-155 ms5957-156 ms5957-157 ms5957-158 ms5957-159 ms5957-160 ms5957-161 ms5957-162 ms5957-163 ms5957-164 ms5957-165 ms5957-166 Factors Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:L | grouping:B Sodium level:H | grouping:A Sodium level:L | grouping:B Sodium level:H | grouping:B Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:H | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:B Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:A Sodium level:L | grouping:B Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:L | grouping:B Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:H | grouping:B Sodium level:H | grouping:A Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:B Sodium level:H | grouping:B Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:H | grouping:A Sodium level:L | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:L | grouping:B Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:H | grouping:A Sodium level:L | grouping:B Sodium level:H | grouping:B Sodium level:L | grouping:B Sodium level:H | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:H | grouping:A Sodium level:L | grouping:A Sodium level:H | grouping:B Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:L | grouping:B Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:L | grouping:A Sodium level:L | grouping:B Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:L | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:H | grouping:A Sodium level:L | grouping:A Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:H | grouping:A Sodium level:L | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:L | grouping:B Sodium level:H | grouping:B Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:B Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:H | grouping:B Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:A Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:L | grouping:B Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:A Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Sodium level:H | grouping:B Acetylcholine 0.04 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.03 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.10 0.00 0.00 0.03 0.00 0.27 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.03 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.05 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.03 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.05 0.00 0.00 0.00 0.00 0.00 Adenosine 23.1 17.4 1.8 1.3 43.5 7.1 49.2 35.5 106.3 41.4 6.5 47.7 66.3 9.1 151.7 2.6 1.3 14.7 81.1 8.5 14.6 2.0 243.2 38.6 74.9 151.6 12.4 17.4 21.4 8.9 0.0 1.4 11.7 2.0 15.4 44.0 14.9 8.9 0.6 1.2 79.9 22.4 13.6 157.8 173.9 68.8 10.1 57.7 126.6 104.3 17.2 267.6 50.0 82.5 0.2 2.8 176.1 86.0 2.3 57.5 26.5 2.1 14.6 15.8 1.4 23.0 25.0 23.8 2.8 10.5 9.6 43.7 37.1 25.8 3.5 4.8 10.1 4.5 10.2 24.4 101.9 4.5 30.4 98.0 34.3 79.5 11.5 35.0 87.8 20.8 26.2 3.4 3.1 12.8 39.3 23.1 40.9 8.5 291.0 4.3 23.0 11.2 0.6 1.8 5.1 4.6 4.8 1.0 0.4 23.5 67.1 26.8 0.4 4.0 0.9 1.0 14.1 8.4 14.4 19.3 2.5 26.0 1.2 2.3 13.5 0.3 0.3 13.1 21.8 7.4 10.9 5.6 0.4 25.3 0.8 1.7 23.1 11.4 20.2 2.1 21.3 17.4 12.4 9.6 90.2 2.8 24.7 20.9 37.1 17.1 23.9 27.9 9.5 23.2 1.1 100.3 1.0 12.5 66.4 2.4 3.9 37.4 110.2 0.4 17.1 5.8 Norepinephrine 0.10 0.26 0.36 0.09 0.33 0.08 0.25 0.28 0.33 0.21 0.17 0.41 0.20 0.10 0.59 0.30 0.18 0.38 0.46 0.25 0.46 0.19 1.05 0.29 0.64 0.51 0.19 0.27 0.19 0.18 0.28 0.14 0.28 0.05 0.29 0.17 0.09 0.09 0.08 0.13 0.00 0.12 0.26 0.32 0.28 0.47 0.23 0.32 0.09 0.44 0.12 0.45 0.17 0.25 0.00 0.18 0.23 0.29 0.13 0.39 0.14 0.03 0.09 0.23 0.13 0.49 0.13 0.11 0.13 0.13 0.11 0.16 0.23 0.15 0.06 0.26 0.18 0.41 0.07 0.22 0.21 0.14 0.21 0.91 0.23 0.18 0.12 0.20 0.25 0.14 0.19 0.04 0.07 0.20 0.15 0.21 0.33 0.28 3.48 0.13 0.09 0.10 0.13 0.01 0.07 0.13 0.15 0.14 0.00 0.30 0.32 0.35 0.14 0.02 0.07 0.28 0.15 0.08 0.13 0.20 0.06 0.20 0.36 0.03 0.11 0.03 0.02 0.11 0.18 0.02 0.26 0.11 0.03 0.17 0.14 0.34 0.16 0.18 0.23 0.12 0.20 0.21 0.02 0.12 0.73 0.05 0.10 0.10 0.31 0.06 0.15 0.23 0.08 0.16 0.18 0.62 0.17 0.07 0.13 0.13 0.14 0.10 0.33 0.03 0.15 0.21 Dopamine 0.09 1.33 1.60 0.08 1.15 0.14 0.92 1.49 1.34 1.04 0.84 1.00 1.18 0.23 2.17 0.41 0.31 1.24 3.42 0.17 2.89 1.01 2.85 0.24 3.53 1.26 0.32 0.72 0.69 0.41 0.75 0.18 0.68 0.30 1.05 0.77 0.08 0.48 0.36 0.57 0.00 0.37 0.68 1.49 2.19 1.86 0.62 0.79 0.47 1.62 0.24 1.48 0.61 1.04 0.04 0.34 1.67 1.03 0.11 2.16 1.23 0.00 0.58 1.17 0.49 0.93 0.09 0.21 0.30 1.11 0.32 0.70 0.98 0.61 0.04 1.38 0.55 1.77 0.07 1.10 0.43 0.18 0.93 1.73 0.89 0.37 0.08 0.99 1.41 0.25 0.71 0.00 0.01 0.09 0.12 0.83 1.94 0.40 4.79 0.04 0.12 0.78 0.94 0.00 0.08 0.70 0.18 0.32 0.00 0.37 1.05 0.90 0.69 0.08 0.09 0.77 0.21 0.57 0.18 0.93 0.05 0.27 0.29 0.02 0.14 0.04 0.04 0.24 0.79 0.00 0.56 0.21 0.03 1.17 0.48 0.50 1.20 1.21 1.38 0.13 1.45 0.78 0.00 0.55 3.75 0.00 0.18 0.87 1.13 0.11 1.05 0.82 0.00 0.31 0.33 4.03 0.65 0.27 0.95 0.85 0.62 0.76 1.28 0.00 0.25 0.77 Serotonin 1.24 1.38 1.49 1.67 2.01 0.28 1.37 2.59 3.27 1.57 0.90 2.04 1.40 0.33 2.55 1.62 0.03 2.10 4.79 0.07 3.42 1.16 4.94 2.94 3.95 3.87 0.85 1.06 1.16 0.85 1.18 2.08 0.78 0.36 2.96 1.20 0.74 0.57 0.52 0.76 1.35 0.70 1.66 1.48 2.86 2.08 1.00 1.77 1.37 2.12 0.71 3.22 0.41 2.87 0.28 2.50 3.48 1.30 1.89 2.06 1.22 0.56 0.78 1.17 0.93 1.09 0.55 1.29 1.22 2.24 0.63 1.40 1.50 0.90 0.13 3.03 0.79 2.08 0.00 1.58 4.37 2.42 2.26 3.54 1.22 1.68 0.02 1.57 2.80 0.90 2.06 0.33 0.75 1.14 1.55 1.01 2.69 3.51 7.26 1.77 1.09 1.11 1.20 0.00 0.69 1.63 0.67 0.63 0.54 1.52 2.30 1.18 0.58 0.48 1.51 1.08 0.76 0.98 0.73 1.78 0.86 1.43 1.79 0.12 0.98 0.27 0.36 0.42 1.60 0.51 0.62 0.22 0.64 0.97 1.00 1.45 0.78 1.10 1.00 0.02 0.54 1.21 2.07 0.75 2.80 0.34 0.74 0.71 1.99 1.48 1.05 1.39 0.39 1.17 1.41 4.21 0.76 0.99 3.12 1.09 1.14 0.92 1.73 0.52 0.97 3.17 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Acetylcholine Adenosine Norepinephrine Dopamine Serotonin METABOLITES_END #END