#METABOLOMICS WORKBENCH araskind_20170619_113620 DATATRACK_ID:1000 STUDY_ID:ST000704 ANALYSIS_ID:AN001094 PROJECT_ID:PR000501 VERSION 1 CREATED_ON July 2, 2017, 10:46 am #PROJECT PR:PROJECT_TITLE The Role of the Microbiome in Alcoholic Liver Disease PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY The effect of acetate on intestinal microorganisms PR:INSTITUTE University of California PR:DEPARTMENT Bioengineering PR:LABORATORY Zengler, Karsten PR:LAST_NAME Zengler PR:FIRST_NAME Karsten PR:ADDRESS San Diego, CA PR:EMAIL kzengler@eng.ucsd.edu PR:PHONE 858-260-9458 #STUDY ST:STUDY_TITLE The effect of acetate on intestinal microorganisms ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY 8 week old B6 Male: HFD+ Glucose, 9 day feeding; Blood collection from tail ST:STUDY_SUMMARY every 3 days; mice were fed glycerol (control) or GTA with increasing ST:STUDY_SUMMARY concentrations; Day 1-3 0.1g/kg; Day 3-6 1g/kg; Day 6-9 6g/kg ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 5 ST:TOTAL_SUBJECTS 5 #SUBJECT SU:SUBJECT_TYPE Mouse SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0019823 S00019591 Diet:GTA feeding | Dose:0.1 g/kg SUBJECT_SAMPLE_FACTORS SU0019824 S00019592 Diet:GTA feeding | Dose:0.1 g/kg SUBJECT_SAMPLE_FACTORS SU0019825 S00019593 Diet:GTA feeding | Dose:0.1 g/kg SUBJECT_SAMPLE_FACTORS SU0019826 S00019594 Diet:Glycerol feeding | Dose:0.1 g/kg SUBJECT_SAMPLE_FACTORS SU0019827 S00019595 Diet:Glycerol feeding | Dose:0.1 g/kg SUBJECT_SAMPLE_FACTORS SU0019823 S00019596 Diet:GTA feeding | Dose:1 g/kg SUBJECT_SAMPLE_FACTORS SU0019824 S00019597 Diet:GTA feeding | Dose:1 g/kg SUBJECT_SAMPLE_FACTORS SU0019826 S00019598 Diet:Glycerol feeding | Dose:1 g/kg SUBJECT_SAMPLE_FACTORS SU0019827 S00019599 Diet:Glycerol feeding | Dose:1 g/kg SUBJECT_SAMPLE_FACTORS SU0019823 S00019600 Diet:GTA feeding | Dose:6 g/kg SUBJECT_SAMPLE_FACTORS SU0019824 S00019601 Diet:GTA feeding | Dose:6 g/kg SUBJECT_SAMPLE_FACTORS SU0019825 S00019602 Diet:GTA feeding | Dose:6 g/kg SUBJECT_SAMPLE_FACTORS SU0019826 S00019603 Diet:Glycerol feeding | Dose:6 g/kg SUBJECT_SAMPLE_FACTORS SU0019827 S00019604 Diet:Glycerol feeding | Dose:6 g/kg #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A007 - Short Chain Fatty Acids.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890N CH:COLUMN_NAME Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A007 - Short Chain Fatty Acids.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples S00019591 S00019592 S00019593 S00019594 S00019595 S00019596 S00019597 S00019598 S00019599 S00019600 S00019601 S00019602 S00019603 S00019604 Factors Diet:GTA feeding | Dose:0.1 g/kg Diet:GTA feeding | Dose:0.1 g/kg Diet:GTA feeding | Dose:0.1 g/kg Diet:Glycerol feeding | Dose:0.1 g/kg Diet:Glycerol feeding | Dose:0.1 g/kg Diet:GTA feeding | Dose:1 g/kg Diet:GTA feeding | Dose:1 g/kg Diet:Glycerol feeding | Dose:1 g/kg Diet:Glycerol feeding | Dose:1 g/kg Diet:GTA feeding | Dose:6 g/kg Diet:GTA feeding | Dose:6 g/kg Diet:GTA feeding | Dose:6 g/kg Diet:Glycerol feeding | Dose:6 g/kg Diet:Glycerol feeding | Dose:6 g/kg PROPIONIC ACID 24.81893863 8.882650006 10.47848455 16.04074196 14.16516278 10.55334426 11.66915486 3.456446872 6.214108481 10.2852588 13.74932725 17.2947379 21.83593369 5.853698314 ISOVALERIC ACID 1.881999495 .522323663 .754458575 .775784263 1.043907708 1.173535206 .784942131 .712418983 .821966021 .504474636 .472823137 .409916158 .593808801 .344748153 ACETIC ACID 1763.187168 365.8119608 465.5062032 290.2580833 453.0147872 397.9395878 470.3317929 165.4069578 193.0739517 113.9581219 133.6742185 172.8862603 173.9005748 220.924319 BUTYRIC ACID 11.9190315 2.191449034 5.912408489 14.01194373 9.362627466 11.56803807 6.873936887 0 1.158969888 .115006942 1.689545651 2.77689211 1.439247435 .497869662 OCTANOIC ACID 4.111795061 .589768524 .332557254 1.722552599 .769693625 .977356984 .694545711 .457941299 .369022945 .478645499 .404122891 .312673464 .507050378 .646897472 VALERIC ACID 3.314285872 .730922331 .427909924 2.04708648 .321731635 1.085482308 1.413326564 .510097201 .168573821 .336845115 .310681754 .13317533 .283009154 .378116012 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type PROPIONIC ACID 1032 ISOVALERIC ACID 10430 ACETIC ACID 176 BUTYRIC ACID 264 OCTANOIC ACID 379 VALERIC ACID 7991 METABOLITES_END #END