#METABOLOMICS WORKBENCH araskind_20170619_114448 DATATRACK_ID:1003 STUDY_ID:ST000706 ANALYSIS_ID:AN001097 PROJECT_ID:PR000503 VERSION 1 CREATED_ON July 2, 2017, 11:07 am #PROJECT PR:PROJECT_TITLE Effect of DASH Diet on Gut Microbiome PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Effect of DASH Diet on Gut Microbiome PR:INSTITUTE Morehouse School of Medicine PR:DEPARTMENT Clinical Research Center PR:LABORATORY Umeakunne Lab PR:LAST_NAME Umeakunne PR:FIRST_NAME Kayellen PR:ADDRESS Atlanta, GA PR:EMAIL kumeakunne@msm.edu PR:PHONE 404-752-1010 #STUDY ST:STUDY_TITLE Effect of DASH Diet on Gut Microbiome (SCFA from stool) ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY This research will examine the effect of the currently recommended DASH diet ST:STUDY_SUMMARY versus a Vegetarian DASH diet on the gut microbiome and risk for cardiovascular ST:STUDY_SUMMARY disease in pre-hypertensive obese African American women. We will define the gut ST:STUDY_SUMMARY microbiota profile, short chain fatty acid production, breath hydrogen/methane ST:STUDY_SUMMARY response, plasma lipopolysaccharide production and other biomarkers of ST:STUDY_SUMMARY inflammation in response to diet type in obese African American pre-hypertensive ST:STUDY_SUMMARY females at baseline and following placement on the traditional DASH plan or DASH ST:STUDY_SUMMARY Vegetarian diet. We will also define these parameters in African American women ST:STUDY_SUMMARY adhering to a Vegetarian or Vegan Diet. Our goal is to determine how the gut ST:STUDY_SUMMARY microbiome modulates host physiology and immune function in response to diet ST:STUDY_SUMMARY type. By evaluating the effect of a recommended DASH dietary pattern versus a ST:STUDY_SUMMARY Vegetarian DASH plan on the gut microbiome and its fermentation products, we aim ST:STUDY_SUMMARY to identify novel information about how these diet types strategically reduce ST:STUDY_SUMMARY cardiovascular disease risk through gut:microbiota:host interaction. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 5 ST:TOTAL_SUBJECTS 13 ST:STUDY_COMMENTS We will define the gut microbiota profile, short chain fatty acid production, ST:STUDY_COMMENTS breath hydrogen/methane response, plasma lipopolysaccharide production and other ST:STUDY_COMMENTS biomarkers of inflammation in response to diet type in obese African American ST:STUDY_COMMENTS pre-hypertensive females at baseline and following placement on the traditional ST:STUDY_COMMENTS DASH plan or DASH Vegetarian diet. We will also define these parameters in ST:STUDY_COMMENTS African American women adhering to a Vegetarian or Vegan Diet. Our goal is to ST:STUDY_COMMENTS determine how the gut microbiome modulates host physiology and immune function ST:STUDY_COMMENTS in response to diet type. By evaluating the effect of a recommended DASH dietary ST:STUDY_COMMENTS pattern versus a Vegetarian DASH plan on the gut microbiome and its fermentation ST:STUDY_COMMENTS products, we aim to identify novel information about how these diet types ST:STUDY_COMMENTS strategically reduce cardiovascular disease risk through gut:microbiota:host ST:STUDY_COMMENTS interaction. #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0013361 S00019911 GROUP:pre-hypertensive | Timepoint:Baseline SUBJECT_SAMPLE_FACTORS SU0013361 S00019912 GROUP:pre-hypertensive | Timepoint:Final SUBJECT_SAMPLE_FACTORS SU0013362 S00019913 GROUP:pre-hypertensive | Timepoint:Baseline SUBJECT_SAMPLE_FACTORS SU0013362 S00019914 GROUP:pre-hypertensive | Timepoint:Final SUBJECT_SAMPLE_FACTORS SU0013363 S00019915 GROUP:vegetarian | Timepoint:NA SUBJECT_SAMPLE_FACTORS SU0013364 S00019916 GROUP:vegetarian | Timepoint:NA SUBJECT_SAMPLE_FACTORS SU0013365 S00019917 GROUP:vegetarian | Timepoint:NA SUBJECT_SAMPLE_FACTORS SU0013366 S00019918 GROUP:vegetarian | Timepoint:NA SUBJECT_SAMPLE_FACTORS SU0013367 S00019919 GROUP:vegetarian | Timepoint:NA SUBJECT_SAMPLE_FACTORS SU0013368 S00019920 GROUP:vegetarian | Timepoint:NA SUBJECT_SAMPLE_FACTORS SU0013369 S00019921 GROUP:vegetarian | Timepoint:NA SUBJECT_SAMPLE_FACTORS SU0013370 S00019922 GROUP:vegetarian | Timepoint:NA SUBJECT_SAMPLE_FACTORS SU0013371 S00019923 GROUP:vegetarian | Timepoint:NA SUBJECT_SAMPLE_FACTORS SU0013372 S00019924 GROUP:vegetarian | Timepoint:NA SUBJECT_SAMPLE_FACTORS SU9999996 S00020331 GROUP:- | Timepoint:- #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A007 - Short Chain Fatty Acids.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890N CH:COLUMN_NAME Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A007 - Short Chain Fatty Acids.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg MS_METABOLITE_DATA_START Samples S00019911 S00019912 S00019913 S00019914 S00019915 S00019916 S00019917 S00019918 S00019919 S00019920 S00019921 S00019922 S00019923 S00019924 Factors GROUP:pre-hypertensive | Timepoint:Baseline GROUP:pre-hypertensive | Timepoint:Final GROUP:pre-hypertensive | Timepoint:Baseline GROUP:pre-hypertensive | Timepoint:Final GROUP:vegetarian | Timepoint:NA GROUP:vegetarian | Timepoint:NA GROUP:vegetarian | Timepoint:NA GROUP:vegetarian | Timepoint:NA GROUP:vegetarian | Timepoint:NA GROUP:vegetarian | Timepoint:NA GROUP:vegetarian | Timepoint:NA GROUP:vegetarian | Timepoint:NA GROUP:vegetarian | Timepoint:NA GROUP:vegetarian | Timepoint:NA PROPIONIC ACID 8.852236798 11.27271996 17.03812961 14.78126926 16.36067918 13.96667136 16.44197152 8.884039523 9.481304533 17.32633263 28.31857223 14.32819129 13.30631982 19.99695861 ISOVALERIC ACID 3.457113334 3.625089679 2.291167277 2.779638021 2.181763109 3.057268574 3.120600567 2.41064069 6.203278222 2.733310428 3.791872433 2.8387834 2.158547839 3.654262637 ACETIC ACID 18.56150626 26.61320737 42.68108337 39.37739033 35.95992971 50.69160271 65.33690694 64.04033935 51.95017774 53.32053031 59.27061884 39.8394841 70.73166133 56.88307069 BUTYRIC ACID 6.915580233 10.13901386 15.81784213 10.65616711 10.08362003 15.02419468 10.23049748 11.54838117 11.33329436 15.52744651 15.1886497 10.21575242 13.58832323 11.69908892 VALERIC ACID 3.577568723 3.88116141 2.201813285 2.783312711 2.817962031 3.882171896 3.706071313 2.95050583 6.367080467 3.280202867 4.69186696 3.418173322 2.516136008 4.785035801 HEPTANOIC ACID .036158188 .029603602 .025142999 .031193913 .020295859 .11874462 .032062447 .023965516 .030655009 .024707107 .032089551 .081992259 .024006212 .02338342 HEXANOIC ACID .66161492 .542445035 .449052572 .589460473 .39309608 1.838362861 .639891077 .45582264 .559459378 .476105594 .6042068 1.193949944 .460094135 .450508403 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type PROPIONIC ACID 1032 ISOVALERIC ACID 10430 ACETIC ACID 176 BUTYRIC ACID 264 VALERIC ACID 7991 HEPTANOIC ACID 8094 HEXANOIC ACID 8892 METABOLITES_END #END