#METABOLOMICS WORKBENCH araskind_20170619_132403 DATATRACK_ID:1016 STUDY_ID:ST000717 ANALYSIS_ID:AN001121 PROJECT_ID:PR000514 VERSION 1 CREATED_ON July 2, 2017, 1:17 pm #PROJECT PR:PROJECT_TITLE Effect of aging on ENS microenvironment PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Assessing influence of microbiota on ENS with age PR:INSTITUTE Stanford University PR:DEPARTMENT Gastroenterology and Hepatology PR:LABORATORY Habtezion Lab PR:LAST_NAME Habtezion PR:FIRST_NAME Aida PR:ADDRESS Stanford, CA PR:EMAIL aida.habtezion@stanford.edu PR:PHONE 650-736-5555 #STUDY ST:STUDY_TITLE Assessing influence of microbiota on enteric nervous system (ENS) with age ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Freshly collected stool samples were immediately frozen in dry ice and stored at ST:STUDY_SUMMARY -80deg. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 1 #SUBJECT SU:SUBJECT_TYPE Mouse SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0015385 S00023725 Age:young stool SUBJECT_SAMPLE_FACTORS SU0015385 S00023726 Age:young stool SUBJECT_SAMPLE_FACTORS SU0015385 S00023727 Age:young stool SUBJECT_SAMPLE_FACTORS SU0015385 S00023728 Age:young stool SUBJECT_SAMPLE_FACTORS SU0015385 S00023729 Age:young stool SUBJECT_SAMPLE_FACTORS SU0015385 S00023730 Age:young stool SUBJECT_SAMPLE_FACTORS SU0015385 S00023731 Age:old stool SUBJECT_SAMPLE_FACTORS SU0015385 S00023732 Age:old stool SUBJECT_SAMPLE_FACTORS SU0015385 S00023733 Age:old stool SUBJECT_SAMPLE_FACTORS SU0015385 S00023734 Age:old stool SUBJECT_SAMPLE_FACTORS SU0015385 S00023735 Age:old stool SUBJECT_SAMPLE_FACTORS SU0015385 S00023736 Age:old stool #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A007 - Short Chain Fatty Acids.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890N CH:COLUMN_NAME Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A007 - Short Chain Fatty Acids.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg MS_METABOLITE_DATA_START Samples S00023725 S00023726 S00023727 S00023728 S00023729 S00023730 S00023731 S00023732 S00023733 S00023734 S00023735 S00023736 Factors Age:young stool Age:young stool Age:young stool Age:young stool Age:young stool Age:young stool Age:old stool Age:old stool Age:old stool Age:old stool Age:old stool Age:old stool PROPIONIC ACID 3.337806192 1.953088897 1.546781479 2.127732721 2.191130681 2.705228522 1.498801009 1.527559739 1.185419256 1.434447063 2.320918575 4.275983491 ACETIC ACID 14.98266682 17.93174519 13.53871913 15.54364482 25.68091522 21.0355069 10.99272627 16.78504486 9.97700172 8.979759058 18.88884949 14.11550993 BUTYRIC ACID 4.413211233 5.136151343 3.73925281 5.040021617 3.598735721 5.067286641 4.301809575 5.04395886 3.794715619 3.465979501 4.263460274 8.418439874 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type PROPIONIC ACID 1032 ACETIC ACID 176 BUTYRIC ACID 264 METABOLITES_END #END