#METABOLOMICS WORKBENCH araskind_20170619_174847 DATATRACK_ID:1033 STUDY_ID:ST000728 ANALYSIS_ID:AN001141 PROJECT_ID:PR000525 VERSION 1 CREATED_ON July 3, 2017, 10:38 am #PROJECT PR:PROJECT_TITLE Antibiotic treatments and Breast Cancer PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Breast cancer model mouse have gone through bariatric surgery and antibiotics PR:PROJECT_SUMMARY treatments. PR:INSTITUTE University of Michigan PR:DEPARTMENT Surgery PR:LABORATORY Seeley Lab PR:LAST_NAME Seeley PR:FIRST_NAME Randy PR:ADDRESS Ann Arbor, MI PR:EMAIL seeleyrj@umich.edu PR:PHONE 734-615-2880 #STUDY ST:STUDY_TITLE Effects of bariatric and antibiotic treatment surgery on mammary carcinogenesis ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY The purpose of this study is to understand the effects of bariatric surgery on ST:STUDY_SUMMARY mammary carcinogenesis in C3 Tag (1) mouse model. My animals have gone through ST:STUDY_SUMMARY antibiotic treatments in drinking water vs. no abx in their water. Also animals ST:STUDY_SUMMARY have been though vertical sleeve gasteroctomy (VSG) vs sham surgery vs no ST:STUDY_SUMMARY surgery group. It is expected that these surgeries will affect the tumor ST:STUDY_SUMMARY proliferation and growth. One potential explanation can be the changes of SCFAs ST:STUDY_SUMMARY following the bariatric surgery. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 5 ST:TOTAL_SUBJECTS 36 #SUBJECT SU:SUBJECT_TYPE Mouse SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0015748 S00024574 Antibiotic:yes | Surgery:SHAM SUBJECT_SAMPLE_FACTORS SU0015749 S00024575 Antibiotic:yes | Surgery:SHAM SUBJECT_SAMPLE_FACTORS SU0015750 S00024576 Antibiotic:yes | Surgery:SHAM SUBJECT_SAMPLE_FACTORS SU0015751 S00024577 Antibiotic:yes | Surgery:SHAM SUBJECT_SAMPLE_FACTORS SU0015752 S00024578 Antibiotic:yes | Surgery:SHAM SUBJECT_SAMPLE_FACTORS SU0015753 S00024579 Antibiotic:yes | Surgery:SHAM SUBJECT_SAMPLE_FACTORS SU0015754 S00024580 Antibiotic:yes | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015755 S00024581 Antibiotic:yes | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015756 S00024582 Antibiotic:yes | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015757 S00024583 Antibiotic:yes | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015758 S00024584 Antibiotic:yes | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015759 S00024585 Antibiotic:yes | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015760 S00024586 Antibiotic:yes | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015761 S00024587 Antibiotic:yes | Surgery:NONE SUBJECT_SAMPLE_FACTORS SU0015762 S00024588 Antibiotic:yes | Surgery:NONE SUBJECT_SAMPLE_FACTORS SU0015763 S00024589 Antibiotic:yes | Surgery:NONE SUBJECT_SAMPLE_FACTORS SU0015764 S00024590 Antibiotic:yes | Surgery:NONE SUBJECT_SAMPLE_FACTORS SU0015765 S00024591 Antibiotic:yes | Surgery:NONE SUBJECT_SAMPLE_FACTORS SU0015766 S00024592 Antibiotic:yes | Surgery:NONE SUBJECT_SAMPLE_FACTORS SU0015767 S00024593 Antibiotic:no | Surgery:SHAM SUBJECT_SAMPLE_FACTORS SU0015768 S00024594 Antibiotic:no | Surgery:SHAM SUBJECT_SAMPLE_FACTORS SU0015769 S00024595 Antibiotic:no | Surgery:SHAM SUBJECT_SAMPLE_FACTORS SU0015770 S00024596 Antibiotic:no | Surgery:SHAM SUBJECT_SAMPLE_FACTORS SU0015771 S00024597 Antibiotic:no | Surgery:SHAM SUBJECT_SAMPLE_FACTORS SU0015772 S00024598 Antibiotic:no | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015773 S00024599 Antibiotic:no | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015774 S00024600 Antibiotic:no | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015775 S00024601 Antibiotic:no | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015776 S00024602 Antibiotic:no | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015777 S00024603 Antibiotic:no | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015778 S00024604 Antibiotic:no | Surgery:VSG SUBJECT_SAMPLE_FACTORS SU0015779 S00024605 Antibiotic:no | Surgery:NONE SUBJECT_SAMPLE_FACTORS SU0015780 S00024606 Antibiotic:no | Surgery:NONE SUBJECT_SAMPLE_FACTORS SU0015781 S00024607 Antibiotic:no | Surgery:NONE SUBJECT_SAMPLE_FACTORS SU0015782 S00024608 Antibiotic:no | Surgery:NONE SUBJECT_SAMPLE_FACTORS SU0015783 S00024609 Antibiotic:no | Surgery:NONE #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A007 - Short Chain Fatty Acids.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890N CH:COLUMN_NAME Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A007 - Short Chain Fatty Acids.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples S00024574 S00024575 S00024576 S00024577 S00024578 S00024579 S00024580 S00024581 S00024582 S00024583 S00024584 S00024585 S00024586 S00024587 S00024588 S00024589 S00024590 S00024591 S00024592 S00024593 S00024594 S00024595 S00024596 S00024597 S00024598 S00024599 S00024600 S00024601 S00024602 S00024603 S00024604 S00024605 S00024606 S00024607 S00024608 S00024609 Factors Antibiotic:yes | Surgery:SHAM Antibiotic:yes | Surgery:SHAM Antibiotic:yes | Surgery:SHAM Antibiotic:yes | Surgery:SHAM Antibiotic:yes | Surgery:SHAM Antibiotic:yes | Surgery:SHAM Antibiotic:yes | Surgery:VSG Antibiotic:yes | Surgery:VSG Antibiotic:yes | Surgery:VSG Antibiotic:yes | Surgery:VSG Antibiotic:yes | Surgery:VSG Antibiotic:yes | Surgery:VSG Antibiotic:yes | Surgery:VSG Antibiotic:yes | Surgery:NONE Antibiotic:yes | Surgery:NONE Antibiotic:yes | Surgery:NONE Antibiotic:yes | Surgery:NONE Antibiotic:yes | Surgery:NONE Antibiotic:yes | Surgery:NONE Antibiotic:no | Surgery:SHAM Antibiotic:no | Surgery:SHAM Antibiotic:no | Surgery:SHAM Antibiotic:no | Surgery:SHAM Antibiotic:no | Surgery:SHAM Antibiotic:no | Surgery:VSG Antibiotic:no | Surgery:VSG Antibiotic:no | Surgery:VSG Antibiotic:no | Surgery:VSG Antibiotic:no | Surgery:VSG Antibiotic:no | Surgery:VSG Antibiotic:no | Surgery:VSG Antibiotic:no | Surgery:NONE Antibiotic:no | Surgery:NONE Antibiotic:no | Surgery:NONE Antibiotic:no | Surgery:NONE Antibiotic:no | Surgery:NONE PROPIONIC ACID 5.432434303 6.893039505 7.590830619 4.702076013 9.704052375 5.462059007 3.957806637 5.030557957 5.91935985 5.23677784 5.302708869 4.63880698 7.097182117 4.479132694 4.857185384 7.415286369 6.19264653 11.61134852 8.039707325 12.23371219 18.0792507 4.783430233 5.242082265 6.827853394 6.684902026 8.093633347 5.25178575 15.24506922 10.444978 15.17946284 15.32362011 12.24180263 15.24543709 3.832142186 5.400510554 8.384563377 ACETIC ACID 177.9322891 191.9792453 264.4409766 116.1258286 147.5231537 150.2370198 71.37946709 155.8702225 126.5503101 128.6646369 154.1427294 270.0898231 203.9769987 103.6850849 150.9190642 150.0703722 172.481167 211.6133321 180.4650813 286.7994087 335.8978138 206.0276394 172.5134581 173.5795451 160.5979074 207.0516275 145.086147 264.85096 279.844235 302.2752123 301.580251 268.9112359 362.6800722 127.6190479 89.94958208 231.0220242 BUTYRIC ACID 4.936472965 5.461949054 5.378846764 3.27265639 6.693285899 6.280350863 3.52689921 7.376379868 8.070454282 3.204406993 7.225977156 5.044628701 6.460205541 2.958998606 6.524558901 5.989369631 4.41382927 8.156647509 6.394012697 11.80395236 17.67590449 4.473255063 5.04268686 6.8090649 9.958230291 7.836214183 6.12899432 9.377111429 11.51271683 15.8898689 16.94950627 14.24845137 16.13281423 3.631686341 5.214119081 12.00199577 HEXANOIC ACID 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.159901947 0 0 0 0 0 0 0 0 0 5.072759522 2.076515345 1.33027762 0 0 0 0 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type PROPIONIC ACID 1032 ACETIC ACID 176 BUTYRIC ACID 264 HEXANOIC ACID 8892 METABOLITES_END #END