#METABOLOMICS WORKBENCH araskind_20170619_135106 DATATRACK_ID:1031 STUDY_ID:ST000732 ANALYSIS_ID:AN001146 PROJECT_ID:PR000529 VERSION 1 CREATED_ON July 3, 2017, 10:59 am #PROJECT PR:PROJECT_TITLE Determining the role of Nudt7 in liver metabolism PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Determining the role of Nudt7 in liver metabolism PR:INSTITUTE West Virginia University PR:DEPARTMENT Biochemistry and Molecular Biology PR:LABORATORY Leonardi Lab PR:LAST_NAME Leonardi PR:FIRST_NAME Roberta PR:ADDRESS Morgantown, WV PR:EMAIL roleonardi@hsc.wvu.edu PR:PHONE 304-293-7591 #STUDY ST:STUDY_TITLE Acylcarnitine profile in fast-refed livers ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY The aim of this study is to determine whether changes in CoA degradation affect ST:STUDY_SUMMARY the composition of the acylcarntine pool in the liver. 7 wild-type and 7 Nudt7 ST:STUDY_SUMMARY knockout male mice (10-16 weeks of age) on a mixed background were fasted for ST:STUDY_SUMMARY 24h and allowed to refeed for 12-14hours before removing the liver. The liver ST:STUDY_SUMMARY samples were flash-frozen and stored at -80C until used. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 4 #SUBJECT SU:SUBJECT_TYPE MOUSE SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0018569 S00028681 Hours after start of re-feeding:12h | Nudt7 Genotype:WT SUBJECT_SAMPLE_FACTORS SU0018569 S00028682 Hours after start of re-feeding:12h | Nudt7 Genotype:WT SUBJECT_SAMPLE_FACTORS SU0018570 S00028683 Hours after start of re-feeding:12h | Nudt7 Genotype:KO SUBJECT_SAMPLE_FACTORS SU0018570 S00028684 Hours after start of re-feeding:12h | Nudt7 Genotype:KO SUBJECT_SAMPLE_FACTORS SU0018571 S00028685 Hours after start of re-feeding:14h | Nudt7 Genotype:WT SUBJECT_SAMPLE_FACTORS SU0018571 S00028686 Hours after start of re-feeding:14h | Nudt7 Genotype:WT SUBJECT_SAMPLE_FACTORS SU0018571 S00028687 Hours after start of re-feeding:14h | Nudt7 Genotype:WT SUBJECT_SAMPLE_FACTORS SU0018571 S00028688 Hours after start of re-feeding:14h | Nudt7 Genotype:WT SUBJECT_SAMPLE_FACTORS SU0018571 S00028689 Hours after start of re-feeding:14h | Nudt7 Genotype:WT SUBJECT_SAMPLE_FACTORS SU0018572 S00028690 Hours after start of re-feeding:14h | Nudt7 Genotype:KO SUBJECT_SAMPLE_FACTORS SU0018572 S00028691 Hours after start of re-feeding:14h | Nudt7 Genotype:KO SUBJECT_SAMPLE_FACTORS SU0018572 S00028692 Hours after start of re-feeding:14h | Nudt7 Genotype:KO SUBJECT_SAMPLE_FACTORS SU0018572 S00028693 Hours after start of re-feeding:14h | Nudt7 Genotype:KO SUBJECT_SAMPLE_FACTORS SU0018572 S00028694 Hours after start of re-feeding:14h | Nudt7 Genotype:KO #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A008 - Acyl Carnitines.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A008 - Acyl Carnitines.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6490A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg MS_METABOLITE_DATA_START Samples S00028681 S00028682 S00028683 S00028684 S00028685 S00028686 S00028687 S00028688 S00028689 S00028690 S00028691 S00028692 S00028693 S00028694 Factors Hours after start of re-feeding:12h | Nudt7 Genotype:WT Hours after start of re-feeding:12h | Nudt7 Genotype:WT Hours after start of re-feeding:12h | Nudt7 Genotype:KO Hours after start of re-feeding:12h | Nudt7 Genotype:KO Hours after start of re-feeding:14h | Nudt7 Genotype:WT Hours after start of re-feeding:14h | Nudt7 Genotype:WT Hours after start of re-feeding:14h | Nudt7 Genotype:WT Hours after start of re-feeding:14h | Nudt7 Genotype:WT Hours after start of re-feeding:14h | Nudt7 Genotype:WT Hours after start of re-feeding:14h | Nudt7 Genotype:KO Hours after start of re-feeding:14h | Nudt7 Genotype:KO Hours after start of re-feeding:14h | Nudt7 Genotype:KO Hours after start of re-feeding:14h | Nudt7 Genotype:KO Hours after start of re-feeding:14h | Nudt7 Genotype:KO ACETYL-DL-CARNITINE .002237487 .001736013 .00256923 .001543589 .006054311 .004663026 .003489493 .002189554 .003159252 .001957245 .007052435 .001122258 .008998991 .005922299 DECANOYLCARNITINE .0000101584 .0000152168 .00000930903 .0000179417 .0000329152 .0000215931 .00000916665 .0000147602 .00000750283 .00000672813 .000022874 .0000046147 .0000199042 .00000955747 LAUROYLCARNITINE .00000820357 .00000837424 .00000785503 .0000211224 .0000264528 .0000171781 .00000682682 .0000156254 .00000799627 .0000095389 .0000135688 .0000034364 .0000158758 .00000983973 PROPIONYLCARNITINE .002019031 .001295041 .002519342 .003512538 .003998262 .00428888 .002606064 .004451255 .002002217 .003227772 .003510161 .002755696 .003477251 .003194251 L-PALMITOYLCARNITINE .000220007 .000216925 .000274705 .000623216 .000337122 .000370152 .00014502 .000640163 .000227717 .000180165 .00023019 .00010942 .000305257 .000274626 O-OCTANOYLCARNITINE .0000101489 .0000126115 .00000732732 .000014268 .0000311081 .0000133384 .00000989519 .0000122053 .00000483641 .00000488427 .000020989 .00000345967 .0000143997 .00000623553 BUTYRYLCARNITINE .00018536 .000384368 .000238362 .0003494 .000904588 .000234407 .000439155 .000356715 .000319814 .000322428 .000895438 .000180809 .000587677 .000602234 DL-CARNITINE .360253504 .329508488 .362617007 .339893899 .331357431 .308945065 .380018397 .404036512 .299714478 .385283931 .408845018 .303405994 .40722338 .493574937 AC1MIY4G .0000301265 .0000496141 .0000174434 .0000351269 .00012098 .0000338798 .0000523168 .0000404136 .0000147786 .0000130088 .0000826129 .00000958144 .0000344477 .0000169156 ARACHIDONYL CARNITINE .000213776 .000207597 .000190057 .000244328 .000177692 .000193495 .000193903 .000289475 .000161799 .000237555 .000215878 .0000828552 .00025702 .000262462 LINOELAIDYL CARNITINE .000160683 .00017374 .000193438 .000455194 .000391057 .000268528 .000126167 .000689603 .000206864 .00017802 .000236881 .0000845181 .000316633 .000297865 18:3 CARNITINE .0000119036 .0000139205 .0000133555 .0000158656 .0000137591 .0000152164 .0000111321 .0000232776 .0000114265 .0000110639 .0000130535 .00000580934 .0000150926 .0000196432 CIS-4-DECENOYL CARNITINE .00000435825 .00000769343 .0000221069 .0000167274 .0000164735 .0000115163 .00000615067 .0000152523 .00000836204 .00000565891 .000010921 .00000407819 .000012428 .0000103347 ISOVALERYLCARNITINE .000211726 .000475479 .000313896 .000228115 .00072242 .000270209 .000450523 .000393584 .000308812 .000294009 .000645967 .000304847 .0004097 .000460618 MYRISTOYLCARNITINE .0000358465 .0000341926 .0000367826 .000113487 .000100621 .000055911 .0000301858 .0000893871 .0000409378 .0000402422 .000050884 .0000193887 .0000638499 .0000442314 OLEOYLCARNITINE .000197735 .000285799 .000234235 .000402243 .000338478 .000355569 .000182976 .000528166 .000166569 .00014692 .000390991 .00012214 .000364779 .000288478 GLUTARYLCARNITINE .0000238701 .0000674134 .0000340388 .0000311715 .000039522 .0000281891 .0000537461 .0000397258 .0000370419 .0000379598 .0000560524 .0000234979 .0000444084 .0000504688 (11Z,14Z)-ICOSADIENOYLCARNITINE .000000493228 .000000482049 .000000573612 .000000505859 .000000379452 .000000251973 .000000307309 .000000291499 .000000375793 .00000057392 .000000270751 .000000434404 .000000445535 .000000170414 3-HYDROXYLAUROYLCARNITINE .0000108104 .0000196768 .0000190604 .0000324192 .0000933145 .0000675079 .0000148376 .0000287581 .0000243597 .0000103331 .0000467722 .0000102371 .0000663371 .000031673 3-HYDROXYMYRISTOYLCARNITINE .000013379 .0000243858 .0000310375 .0000540207 .000181942 .0000805898 .0000226426 .0000443562 .000036451 .0000138207 .0000644741 .0000150881 .000103116 .0000376592 3-HYDROXYPALMITOYLCARNITINE .0000796823 .000168755 .000210688 .000291762 .000666798 .000306806 .0000873898 .000428227 .000238017 .0000788874 .000231819 .000070728 .000437474 .000235043 3-HYDROXYLINOLEYLCARNITINE .0000639944 .000202781 .000176178 .000261487 .000748142 .000314716 .000104314 .000322034 .00016469 .0000603774 .000335434 .0000842391 .000438382 .000229297 16:1 CARNITINE .0000938649 .0000998522 .0000727527 .000132582 .000187942 .00016522 .0000695394 .00017386 .0000716986 .0000570721 .00009714 .0000428202 .000125514 .0000934193 8:1 CARNITINE .00000187118 .00000372209 .00000289743 .00000552869 .00000517865 .00000289619 .00000227622 .0000075059 .00000394353 .00000245094 .00000314617 .00000206978 .0000037987 .00000417299 14:2 CARNITINE .00000322391 .00000245955 .00000594824 .0000250797 .000021003 .00000927163 .00000294834 .0000206777 .00000626429 .00000535992 .00000677457 .00000203143 .00000817579 .00000574149 12:1 CARNITINE .00000407901 .00000292612 .00000285103 .00000998228 .0000152813 .00000752055 .00000317218 .00000870853 .0000040329 .00000320779 .0000065594 .00000176023 .00000886065 .00000504263 14:1 CARNITINE .0000146558 .0000105535 .00000927238 .0000365682 .0000564611 .0000336071 .0000104545 .0000320769 .0000121622 .0000123314 .0000219502 .00000567499 .0000241342 .0000144242 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type ACETYL-DL-CARNITINE 1 DECANOYLCARNITINE 10245190 LAUROYLCARNITINE 10427569 PROPIONYLCARNITINE 107738 L-PALMITOYLCARNITINE 11953816 O-OCTANOYLCARNITINE 123701 BUTYRYLCARNITINE 213144 DL-CARNITINE 288 AC1MIY4G 3082079 ARACHIDONYL CARNITINE 53477832 LINOELAIDYL CARNITINE 53477834 18:3 CARNITINE 54500892 CIS-4-DECENOYL CARNITINE 57357170 ISOVALERYLCARNITINE 6426851 MYRISTOYLCARNITINE 6426854 OLEOYLCARNITINE 6441392 GLUTARYLCARNITINE 71317118 (11Z,14Z)-ICOSADIENOYLCARNITINE 71464509 3-HYDROXYLAUROYLCARNITINE 71464535 3-HYDROXYMYRISTOYLCARNITINE 71464541 3-HYDROXYPALMITOYLCARNITINE 71464553 3-HYDROXYLINOLEYLCARNITINE 71464556 16:1 CARNITINE 76040365 8:1 CARNITINE 77908888 14:2 CARNITINE 87087940 12:1 CARNITINE 87087953 14:1 CARNITINE 87088002 METABOLITES_END #END