#METABOLOMICS WORKBENCH araskind_20170802_184152 DATATRACK_ID:1185 STUDY_ID:ST000755 ANALYSIS_ID:AN001329 PROJECT_ID:PR000593 VERSION 1 CREATED_ON August 9, 2017, 9:19 am #PROJECT PR:PROJECT_TITLE Regulatory roles of glycolysis and its enzymes in photoreceptor survival PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY The goals specific to this proposal are to understand how the metabolic flux PR:PROJECT_SUMMARY through glycolysis and its associated pathways, such as oxidative PR:PROJECT_SUMMARY phosphorylation and pentose phosphate pathway, is regulated during periods of PR:PROJECT_SUMMARY photoreceptor stress. PR:INSTITUTE University of Michigan PR:DEPARTMENT Ophthamology and Visual Sciences PR:LABORATORY Besirli Lab PR:LAST_NAME Besirli PR:FIRST_NAME Cagri PR:ADDRESS Ann Arbor, MI PR:EMAIL cbesirli@umich.edu PR:PHONE 734-615-6099 #STUDY ST:STUDY_TITLE Rat Retinal Detachment Metabolomics Timecourse (part II) ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Detachments were created in adult male Brown-Norway rats (300?400 g; Charles ST:STUDY_SUMMARY River Laboratories, Wilmington, MA). Briefly, rodents were anesthetized with a ST:STUDY_SUMMARY 50:50 mix of ketamine/xylazine, and pupils were dilated with topical ST:STUDY_SUMMARY phenylephrine (2.5%) and tropicamide (1%). A 20-gauge microvitreoretinal blade ST:STUDY_SUMMARY was used to create a sclerotomy 2 mm posterior to the limbus, carefully avoiding ST:STUDY_SUMMARY lens damage. A subretinal injector (Glaser, 32-gauge tip; BD Ophthalmic Systems, ST:STUDY_SUMMARY Franklin Lakes, NJ) was introduced through the sclerotomy into the vitreous ST:STUDY_SUMMARY cavity and then through a peripheral retinotomy into the subretinal space. ST:STUDY_SUMMARY Sodium hyaluronate (10 mg/mL, Healon O.D Abbott Medical Optics, Uppsala, ST:STUDY_SUMMARY Sweden) was slowly injected to detach the neurosensory retina from the ST:STUDY_SUMMARY underlying RPE. In all experiments, approximately one third to one half of the ST:STUDY_SUMMARY neurosensory retina was detached. Detachments were created in the left eye. The ST:STUDY_SUMMARY right eye served as the control, with all the steps of the procedure performed ST:STUDY_SUMMARY except for introduction of the subretinal injector and injection of the sodium ST:STUDY_SUMMARY hyaluronate. At varying intervals after creation of the detachment, the animals ST:STUDY_SUMMARY were euthanatized, and the eyes were enucleated.The retina was then dissected ST:STUDY_SUMMARY away from the retinal pigment epithelium taking just the detached portion in ST:STUDY_SUMMARY those eyes experimentally detached. All experiments were performed in accordance ST:STUDY_SUMMARY with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research ST:STUDY_SUMMARY and the guidelines established by the University Committee on Use and Care of ST:STUDY_SUMMARY Animals of the University of Michigan. Detachments were created in adult male ST:STUDY_SUMMARY Brown-Norway rats (300?400 g; Charles River Laboratories, Wilmington, MA). ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 10 ST:TOTAL_SUBJECTS 27 ST:STUDY_COMMENTS All experiments were performed in accordance with the ARVO Statement for the Use ST:STUDY_COMMENTS of Animals in Ophthalmic and Vision Research and the guidelines established by ST:STUDY_COMMENTS the University Committee on Use and Care of Animals of the University of ST:STUDY_COMMENTS Michigan. Detachments were created in adult male Brown-Norway rats (300?400 g; ST:STUDY_COMMENTS Charles River Laboratories, Wilmington, MA). #SUBJECT SU:SUBJECT_TYPE RAT SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10114 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0016280 S00026076 Retina Attachment:Detached | Time:5 min SUBJECT_SAMPLE_FACTORS SU0016280 S00026077 Retina Attachment:Attached | Time:5 min SUBJECT_SAMPLE_FACTORS SU0016282 S00026078 Retina Attachment:Detached | Time:5 min SUBJECT_SAMPLE_FACTORS SU0016282 S00026079 Retina Attachment:Attached | Time:5 min SUBJECT_SAMPLE_FACTORS SU0016284 S00026080 Retina Attachment:Detached | Time:5 min SUBJECT_SAMPLE_FACTORS SU0016284 S00026081 Retina Attachment:Attached | Time:5 min SUBJECT_SAMPLE_FACTORS SU0016286 S00026082 Retina Attachment:Detached | Time:15 min SUBJECT_SAMPLE_FACTORS SU0016286 S00026083 Retina Attachment:Attached | Time:15 min SUBJECT_SAMPLE_FACTORS SU0016288 S00026084 Retina Attachment:Detached | Time:15 min SUBJECT_SAMPLE_FACTORS SU0016288 S00026085 Retina Attachment:Attached | Time:15 min SUBJECT_SAMPLE_FACTORS SU0016290 S00026086 Retina Attachment:Detached | Time:15 min SUBJECT_SAMPLE_FACTORS SU0016290 S00026087 Retina Attachment:Attached | Time:15 min SUBJECT_SAMPLE_FACTORS SU0016292 S00026088 Retina Attachment:Detached | Time:30 min SUBJECT_SAMPLE_FACTORS SU0016292 S00026089 Retina Attachment:Attached | Time:30 min SUBJECT_SAMPLE_FACTORS SU0016294 S00026090 Retina Attachment:Detached | Time:30 min SUBJECT_SAMPLE_FACTORS SU0016294 S00026091 Retina Attachment:Attached | Time:30 min SUBJECT_SAMPLE_FACTORS SU0016296 S00026092 Retina Attachment:Detached | Time:30 min SUBJECT_SAMPLE_FACTORS SU0016296 S00026093 Retina Attachment:Attached | Time:30 min SUBJECT_SAMPLE_FACTORS SU0016298 S00026094 Retina Attachment:Detached | Time:45 min SUBJECT_SAMPLE_FACTORS SU0016298 S00026095 Retina Attachment:Attached | Time:45 min SUBJECT_SAMPLE_FACTORS SU0016300 S00026096 Retina Attachment:Detached | Time:45 min SUBJECT_SAMPLE_FACTORS SU0016300 S00026097 Retina Attachment:Attached | Time:45 min SUBJECT_SAMPLE_FACTORS SU0016302 S00026098 Retina Attachment:Detached | Time:45 min SUBJECT_SAMPLE_FACTORS SU0016302 S00026099 Retina Attachment:Attached | Time:45 min SUBJECT_SAMPLE_FACTORS SU0016304 S00026100 Retina Attachment:Detached | Time:90 min SUBJECT_SAMPLE_FACTORS SU0016304 S00026101 Retina Attachment:Attached | Time:90 min SUBJECT_SAMPLE_FACTORS SU0016306 S00026102 Retina Attachment:Detached | Time:90 min SUBJECT_SAMPLE_FACTORS SU0016306 S00026103 Retina Attachment:Attached | Time:90 min SUBJECT_SAMPLE_FACTORS SU0016308 S00026104 Retina Attachment:Detached | Time:90 min SUBJECT_SAMPLE_FACTORS SU0016308 S00026105 Retina Attachment:Attached | Time:90 min SUBJECT_SAMPLE_FACTORS SU0016310 S00026106 Retina Attachment:Detached | Time:3 hr SUBJECT_SAMPLE_FACTORS SU0016310 S00026107 Retina Attachment:Attached | Time:3 hr SUBJECT_SAMPLE_FACTORS SU0016312 S00026108 Retina Attachment:Detached | Time:3 hr SUBJECT_SAMPLE_FACTORS SU0016312 S00026109 Retina Attachment:Attached | Time:3 hr SUBJECT_SAMPLE_FACTORS SU0016314 S00026110 Retina Attachment:Detached | Time:3 hr SUBJECT_SAMPLE_FACTORS SU0016314 S00026111 Retina Attachment:Attached | Time:3 hr SUBJECT_SAMPLE_FACTORS SU0016316 S00026112 Retina Attachment:Detached | Time:5 hr SUBJECT_SAMPLE_FACTORS SU0016316 S00026113 Retina Attachment:Attached | Time:5 hr SUBJECT_SAMPLE_FACTORS SU0016318 S00026114 Retina Attachment:Detached | Time:5 hr SUBJECT_SAMPLE_FACTORS SU0016318 S00026115 Retina Attachment:Attached | Time:5 hr SUBJECT_SAMPLE_FACTORS SU0016320 S00026116 Retina Attachment:Detached | Time:5 hr SUBJECT_SAMPLE_FACTORS SU0016320 S00026117 Retina Attachment:Attached | Time:5 hr SUBJECT_SAMPLE_FACTORS SU0016322 S00026118 Retina Attachment:Detached | Time:16 hr SUBJECT_SAMPLE_FACTORS SU0016322 S00026119 Retina Attachment:Attached | Time:16 hr SUBJECT_SAMPLE_FACTORS SU0016324 S00026120 Retina Attachment:Detached | Time:16 hr SUBJECT_SAMPLE_FACTORS SU0016324 S00026121 Retina Attachment:Attached | Time:16 hr SUBJECT_SAMPLE_FACTORS SU0016326 S00026122 Retina Attachment:Detached | Time:16 hr SUBJECT_SAMPLE_FACTORS SU0016326 S00026123 Retina Attachment:Attached | Time:16 hr SUBJECT_SAMPLE_FACTORS SU0016328 S00026124 Retina Attachment:Detached | Time:16 hr SUBJECT_SAMPLE_FACTORS SU0016328 S00026125 Retina Attachment:Attached | Time:16 hr SUBJECT_SAMPLE_FACTORS SU0016330 S00026126 Retina Attachment:Detached | Time:16 hr SUBJECT_SAMPLE_FACTORS SU0016330 S00026127 Retina Attachment:Attached | Time:16 hr SUBJECT_SAMPLE_FACTORS SU0016332 S00026128 Retina Attachment:Detached | Time:16 hr SUBJECT_SAMPLE_FACTORS SU0016332 S00026129 Retina Attachment:Attached | Time:16 hr #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A011_Glycolysis-TCA-nucleotides.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A011 - Glycolysis-TCA-nucleotides.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6520B QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg MS_METABOLITE_DATA_START Samples S00026076 S00026077 S00026078 S00026079 S00026080 S00026081 S00026082 S00026083 S00026084 S00026085 S00026086 S00026087 S00026088 S00026089 S00026090 S00026091 S00026092 S00026093 S00026094 S00026095 S00026096 S00026097 S00026098 S00026099 S00026100 S00026101 S00026102 S00026103 S00026104 S00026105 S00026106 S00026107 S00026108 S00026109 S00026110 S00026111 S00026112 S00026113 S00026114 S00026115 S00026116 S00026117 S00026118 S00026119 S00026120 S00026121 S00026122 S00026123 S00026124 S00026125 S00026126 S00026127 S00026128 S00026129 Factors Retina Attachment:Detached | Time:5 min Retina Attachment:Attached | Time:5 min Retina Attachment:Detached | Time:5 min Retina Attachment:Attached | Time:5 min Retina Attachment:Detached | Time:5 min Retina Attachment:Attached | Time:5 min Retina Attachment:Detached | Time:15 min Retina Attachment:Attached | Time:15 min Retina Attachment:Detached | Time:15 min Retina Attachment:Attached | Time:15 min Retina Attachment:Detached | Time:15 min Retina Attachment:Attached | Time:15 min Retina Attachment:Detached | Time:30 min Retina Attachment:Attached | Time:30 min Retina Attachment:Detached | Time:30 min Retina Attachment:Attached | Time:30 min Retina Attachment:Detached | Time:30 min Retina Attachment:Attached | Time:30 min Retina Attachment:Detached | Time:45 min Retina Attachment:Attached | Time:45 min Retina Attachment:Detached | Time:45 min Retina Attachment:Attached | Time:45 min Retina Attachment:Detached | Time:45 min Retina Attachment:Attached | Time:45 min Retina Attachment:Detached | Time:90 min Retina Attachment:Attached | Time:90 min Retina Attachment:Detached | Time:90 min Retina Attachment:Attached | Time:90 min Retina Attachment:Detached | Time:90 min Retina Attachment:Attached | Time:90 min Retina Attachment:Detached | Time:3 hr Retina Attachment:Attached | Time:3 hr Retina Attachment:Detached | Time:3 hr Retina Attachment:Attached | Time:3 hr Retina Attachment:Detached | Time:3 hr Retina Attachment:Attached | Time:3 hr Retina Attachment:Detached | Time:5 hr Retina Attachment:Attached | Time:5 hr Retina Attachment:Detached | Time:5 hr Retina Attachment:Attached | Time:5 hr Retina Attachment:Detached | Time:5 hr Retina Attachment:Attached | Time:5 hr Retina Attachment:Detached | Time:16 hr Retina Attachment:Attached | Time:16 hr Retina Attachment:Detached | Time:16 hr Retina Attachment:Attached | Time:16 hr Retina Attachment:Detached | Time:16 hr Retina Attachment:Attached | Time:16 hr Retina Attachment:Detached | Time:16 hr Retina Attachment:Attached | Time:16 hr Retina Attachment:Detached | Time:16 hr Retina Attachment:Attached | Time:16 hr Retina Attachment:Detached | Time:16 hr Retina Attachment:Attached | Time:16 hr BETA-NAD+ .043628029 .034167775 .024728483 .061926931 .028213598 .076895868 .019750724 .058458963 .027795494 .063176923 .058441874 .06778458 .029994594 .029923822 .029449697 .045430736 .026465977 .069163223 .032267759 .055227994 .018744925 .052262443 .028083481 .055087537 .018996101 .054524501 .018183555 .05231251 .028542303 .000570283 .031871251 .0597688 .018511064 .055594889 .026486574 .050341998 .019269418 .055931112 .013299298 .058210899 .016092346 .029567935 .021690646 .044543774 .014010161 .041379017 .016128067 .051010191 .010165818 .037168312 .020707656 .051057751 .060542143 .044894129 COENZYME II .003008333 .003585323 .001632566 .004421331 .001770121 .005020062 .00125513 .003290387 .001431957 .002708061 .00400542 .005857123 .002094999 .002325635 .002107579 .003284401 .001598825 .004467172 .002479183 .003823269 .001456083 .00369198 .001663434 .003576994 .001895337 .004330223 .001361222 .003557271 .001732687 .003265845 .002184755 .0050538 .001379285 .003976447 .002025008 .004139722 .001567699 .004188239 .00120733 .004256293 .001269137 .002837391 .001974922 .003236015 .00144899 .003144363 .001338897 .004016251 .001177124 .003276414 .001935702 .003676098 .004289032 .004368283 SEDOHEPTULOSE 7-PHOSPHATE .00507613 .072674895 .008964997 .022392472 .008139852 .013444079 .007627782 .01310035 .011613855 .014340473 .005917204 .032531511 .008883112 .0153088 .015340456 .0204629 .005390933 .022903824 .007600675 .023549745 .008360443 .016601284 .005677239 .027238091 .004865777 .024019939 .004273794 .017367592 .008274617 .01448702 .002342514 .062097691 .005721532 .014403499 .00463038 .021584287 .011603415 .026499887 .006233456 .016559079 .004434385 .008687377 .004756887 .00687651 .006666637 .012282181 .00397664 .043189045 .026188771 .020348409 .002120607 .005102245 .003401771 .007116594 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) .028713667 .018806541 .013529468 .021733341 .016449628 .043519039 .007457335 .014630073 .007254524 .009391395 .044386635 .029814824 .011225177 .008606081 .011267202 .019814556 .007113288 .019727005 .039274909 .01349262 .011630039 .014200252 .007076148 .020003868 .008567337 .015784082 .012820566 .01204912 .008654479 .012197671 .036240455 .016624879 .013457266 .009235055 .01785178 .013763795 .006227075 .008549651 .007119235 .013173148 .005654449 .009085716 .018670404 .00711635 .008080958 .006380948 .006592538 .01130195 .006986636 .007394412 .023362879 .009861111 .02881301 .016836207 NADP-REDUCED .003762768 .002629283 .023175206 .004058489 .007450087 .005703297 .00822998 .005720909 .027433387 .000525672 .005124106 .006767131 .01177105 .006674098 .006857981 .007868539 .021766584 .003666523 .018284647 .003903071 .016946881 .006349266 .020605148 .008388184 .00328697 .019763302 .007663362 .025976087 .005839779 .001896182 .015365957 .015330381 .003815212 .007723544 .002407998 .007185692 .003101393 .005582267 .00190274 .011708586 .000773186 .012578935 .002509541 .007629137 .001396847 .011796513 .000741639 .006032287 .007161588 CITRIC ACID .124181299 .398500014 .090600656 .629113633 .121557039 .379466765 .384203679 .384554958 .320573041 1.077416894 .122042286 .478621598 .211941699 .701036159 .219693645 .269728049 .652967263 .803458014 .17721755 .593364253 .174140699 .616292869 .529597231 .649396198 .123991099 .421594217 .148924587 .703078594 .602542609 1.099377437 .077573424 .563691767 .151545073 .873919071 .108833511 1.182443179 .432995073 .560482036 .31844536 .338979074 .519450117 .241434805 .17964633 .277676205 .159291222 .8414082 .16011389 .367588863 .191643713 1.013246195 .077332741 .152854695 .156265686 .261146004 NADH .0046445 .004641109 .006229091 .01186786 .008525365 .012529944 .003691386 .01315585 .004783252 .014995023 .004781906 .010669753 .007995986 .005099649 .004623158 .007393825 .00399247 .013672591 .006670213 .009240341 .002624758 .008652312 .003495965 .009725482 .001038484 .005905153 .010505952 .009828925 .004618309 .014083959 .002140879 .005049349 .003488542 .007110225 .001536787 .010993705 .0026557 .006057925 .001915841 .00591854 .001660694 .005678257 .006410211 .00953243 .00241 .005334308 .00401581 .005037414 .000859582 .005986966 .001222025 .006045677 .006774705 .00935284 FAD .006860485 .007841085 .006838127 .007957908 .006708537 .0079491 .006447853 .007213788 .006693078 .007743285 .00679796 .007837337 .006942193 .007005761 .006638466 .006755186 .006573229 .007812006 .006779516 .007379955 .0064341 .006996183 .006555941 .007017641 .006092058 .006882463 .006282162 .007003052 .006633845 .00724673 .006381573 .007422978 .006531626 .006955043 .006240793 .007103814 .006363476 .007019897 .006358079 .007388736 .006447005 .00702516 .00662295 .006783548 .006435528 .00739439 .006740034 .00740067 .006588946 .007598827 .006558886 .007257707 .007096747 .00745018 MALIC ACID .071974827 .097079904 .091674762 .209117812 .066312035 .184485896 .02328193 .113160785 .035392919 .063866296 .069613143 .111520427 .086030213 .089802186 .048582577 .151089515 .024118343 .121928419 .132709618 .117126272 .033027977 .15311514 .024090676 .137109823 .10416864 .07481083 .094743563 .10498303 .045888525 .144387226 .056583475 .110693844 .095781463 .207320431 .06786931 .166327329 .080250542 .093626652 .049356986 .160954964 .024373878 .213236749 .214119972 .392755448 .12044869 .142230936 .18797336 .172732062 .082207495 .187874251 .118600671 .413917195 .14376522 .449899845 ADENOSINE TRIPHOSPHATE .099366931 .019103636 .031462394 .031974068 .016921894 .1541691 .015722816 .019529786 .011338193 .024452391 .304405417 .07121858 .016808593 .024677714 .019041766 .017373817 .015255909 .031723035 .147150542 .023000956 .008475463 .023063854 .015438761 .024689553 .035271688 .01851966 .029745985 .022776989 .01927736 .02587578 .162883981 .025238041 .025760076 .020493992 .147241614 .040541127 .015445201 .018738096 .012311906 .027890451 .011161651 .022992526 .099127735 .000555924 .014437768 .0217121 .013252381 .014633906 .009799067 .037483943 .083748293 .070070312 .143414713 .125554165 ROBISON ESTER .092270872 .298724224 .068880887 .096967995 .063406965 .205794505 .031679236 .131585523 .054977952 .082351753 .130801495 .174121866 .070238914 .063347507 .037691805 .112751922 .032427444 .113916993 .110839463 .10080882 .038148353 .078883635 .034415309 .097070522 .056313602 .081973353 .069418099 .091877814 .050231127 .089813769 .055416927 .118698248 .070117851 .080909355 .09706951 .115952559 .051340333 .113154525 .042773396 .119803975 .036244547 .084329536 .10842829 .131052995 .062088114 .084383695 .074729537 .108280464 .049770412 .127759597 .048812077 .127339631 .055742 .160002003 SUCROSE .278846434 1.021850111 .155343705 .844951253 .243461679 .85066391 .134547883 .787146014 .184068075 .83488209 .352772953 1.422574839 .313674242 .395816779 .138655641 .801962424 .19642485 1.295991579 .264681609 1.085892477 .241899007 .700913654 .124045895 .845369606 .088626699 .942394276 .124362115 .790695005 .167730885 .513338499 .206201839 1.288098616 .215844582 .654113251 .333106221 1.026593481 .199835603 1.13490801 .11575859 1.059685265 .06321326 .454012585 .161810241 .261333751 .223916998 .534006464 .12726827 .869993137 .157655054 .557593274 .095927205 .260992749 .378374695 .28962007 ADENOSINE 5'-DIPHOSPHATE .084519516 .088095543 .059953585 .067591645 .031827129 .29086509 .007676592 .109823704 .011489218 .062022114 .187941302 .246885221 .024438583 .034396825 .010852317 .084656837 .013178328 .07840266 .097767546 .048837304 .010691508 .049608879 .009296911 .046681289 .015904557 .05077812 .019008257 .05072541 .011342617 .054226446 .040579947 .066887079 .024999205 .049745989 .067429805 .053491701 .006039129 .058253988 .003004302 .096263523 .005233863 .04419571 .036252576 .041253866 .016752084 .030795021 .014126085 .032686855 .003033419 .011915021 .021833843 .115009749 .12418568 .045754898 ADENOSINE 5'-MONOPHOSPHATE .122653451 .212365533 .072695832 .040354031 .05681465 .203358738 .004011274 .19410168 .007395203 .04356594 .097485062 .378764067 .015487053 .02128196 .00492303 .159521748 .005965355 .039070945 .041423059 .038026117 .008236031 .029031896 .00470594 .025082959 .006088877 .066078785 .010077491 .022109061 .005678091 .02322489 .020969009 .100713416 .025932745 .032215947 .030041113 .020400668 .004233444 .041510077 .002128843 .131457082 .003507654 .050038948 .008858616 .012128088 .014226101 .012675388 .008442739 .021853315 .003053597 .00727042 .005119119 .049759408 .070720373 .006591573 GLYCERATE 3-PHOSPHATE .040950444 .062207443 .014243244 .052121959 .010298323 .043709426 .219230179 .023371686 .07843323 .058424402 .053219972 .045784506 .018488744 .059098744 .0680721 .015000459 .048941169 .075204752 .048756385 .049743189 .013953554 .045847455 .068565084 .057938301 .029392911 .024322445 .027357762 .046595203 .156175624 .062521407 .04629014 .032179894 .025681434 .038850124 .09493329 .084940361 .184484538 .041682289 .168667162 .024277514 .035076541 .019052258 .089327581 .029244311 .013201306 .070389811 .021166034 .032425333 .078689675 .181561522 .01713945 .020706251 .030452138 .081726203 RIBOSE PHOSPHATE .057306469 .277509006 .224984492 .400924763 .222545118 .314383256 .116078134 .309388525 .15928389 .210840528 .0345083 .29838411 .30832085 .195818314 .163147793 .357142925 .141090774 .378815187 .282807894 .387774013 .146975923 .347751022 .149855596 .40294758 .278795125 .342745273 .231315207 .338699124 .191626149 .359356525 .043000334 .380842836 .23149304 .353844224 .058316885 .397881061 .220261341 .381442559 .15140322 .398799533 .147494594 .333203012 .285691831 .495555218 .282125539 .325415477 .323797702 .427939381 .205151046 .387317512 .19637499 .529782482 .092732475 .610256762 6-PHOSPHOGLUCONIC ACID .001351053 .003583616 .000757406 .002540124 .000638517 .001678804 .001439545 .001331545 .002229844 .003216651 .002296547 .001744238 .001689449 .002017162 .001778202 .001130314 .001912258 .002789867 .001277615 .002368792 .00083107 .002925103 .002614673 .002382242 .000976805 .001722663 .001170118 .003086349 .002654234 .004280351 .000844974 .001574064 .001030305 .003236744 .000699689 .003500272 .001621416 .002530062 .001363019 .00191902 .001369443 .001458474 .000738094 .001459868 .000611889 .002420432 .000877961 .001352651 .000720906 .002246907 .000788176 .001159884 .00161473 .001182498 ACETOACETYL-COA .000389698 .001039051 .000640112 .002681789 .000685376 .001699501 .000890433 .001023106 .000988938 .002781695 .001055449 .002181865 .001130909 .001756944 .000954698 .000958074 .001250936 .002207785 .001633044 .002377658 .000743491 .001753204 .000966031 .002222422 .000867667 .001727352 .000827449 .002196153 .001084311 .002710352 .00042982 .001938866 .000953969 .002226738 .00065461 .002408377 .000676057 .001943957 .000571248 .001656999 .000673966 .001413663 .000911745 .001605165 .000955662 .001467644 .000733142 .001642969 .000326181 .001395845 .000283445 .001331265 .000923614 .001258166 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type BETA-NAD+ 15938971 COENZYME II 15938972 SEDOHEPTULOSE 7-PHOSPHATE 165007 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 21125049 NADP-REDUCED 22833512 CITRIC ACID 311 NADH 439153 FAD 46906035 MALIC ACID 525 ADENOSINE TRIPHOSPHATE 5957 ROBISON ESTER 5958 SUCROSE 5988 ADENOSINE 5'-DIPHOSPHATE 6022 ADENOSINE 5'-MONOPHOSPHATE 6083 GLYCERATE 3-PHOSPHATE 724 RIBOSE PHOSPHATE 77982 6-PHOSPHOGLUCONIC ACID 91493 ACETOACETYL-COA 92153 METABOLITES_END #END