#METABOLOMICS WORKBENCH michaelsa93_20170719_121530 DATATRACK_ID:1155 STUDY_ID:ST000813 ANALYSIS_ID:AN001286 PROJECT_ID:PR000576 VERSION 1 CREATED_ON July 24, 2017, 12:45 pm #PROJECT PR:PROJECT_TITLE Insights into myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) PR:PROJECT_TITLE phenotypes through comprehensive metabolomics PR:PROJECT_SUMMARY The pathogenesis of ME/CFS, a disease characterized by unexplained debilitating PR:PROJECT_SUMMARY fatigue, cognitive dysfunction, sleep disturbances, orthostatic intolerance, PR:PROJECT_SUMMARY fever, lymphadenopathy and irritable bowel syndrome (IBS), is poorly understood. PR:PROJECT_SUMMARY There are no validated diagnostic tests or interventions to mitigate disease. PR:PROJECT_SUMMARY Here we report association modeling, biomarker discovery, biochemical enrichment PR:PROJECT_SUMMARY analysis and topological network visualization of plasma metabolomic, fecal PR:PROJECT_SUMMARY bacterial metagenomic and clinical data from 50 ME/CFS patients and 50 healthy PR:PROJECT_SUMMARY controls. PR:INSTITUTE Columbia University PR:DEPARTMENT Center for Infection and Immunity, Mailman School of Public Health PR:LAST_NAME Lipkin PR:FIRST_NAME Ian PR:ADDRESS 722 West 168th Street, Room 1703a, New York, NY USA 10032 PR:EMAIL wil2001@columbia.edu PR:PHONE 212-342-9044 #STUDY ST:STUDY_TITLE Insights into myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) ST:STUDY_TITLE phenotypes through comprehensive metabolomics (part IV) ST:STUDY_TYPE Observational ST:STUDY_SUMMARY The pathogenesis of ME/CFS, a disease characterized by unexplained debilitating ST:STUDY_SUMMARY fatigue, cognitive dysfunction, sleep disturbances, orthostatic intolerance, ST:STUDY_SUMMARY fever, lymphadenopathy and irritable bowel syndrome (IBS), is poorly understood. ST:STUDY_SUMMARY There are no validated diagnostic tests or interventions to mitigate disease. ST:STUDY_SUMMARY Here we report association modeling, biomarker discovery, biochemical enrichment ST:STUDY_SUMMARY analysis and topological network visualization of plasma metabolomic, fecal ST:STUDY_SUMMARY bacterial metagenomic and clinical data from 50 ME/CFS patients and 50 healthy ST:STUDY_SUMMARY controls. Through targeted and untargeted metabolomics analyses we confirm ST:STUDY_SUMMARY earlier reports of specific alterations in plasma levels of choline, carnitine ST:STUDY_SUMMARY and complex lipid metabolism in ME/CFS. We also demonstrate that patients with ST:STUDY_SUMMARY ME/CFS and IBS have a unique metabolomic profile that includes increased plasma ST:STUDY_SUMMARY levels of ceramide, a waxy lipid implicated in suppression of electron ST:STUDY_SUMMARY transport, insulin and leptin resistance and apoptosis. Integration of fecal ST:STUDY_SUMMARY metagenomic and plasma metabolomic data resulted in a stronger predictive model ST:STUDY_SUMMARY of ME/CFS (cross-validated AUC=0.836) than either metagenomic (cross-validated ST:STUDY_SUMMARY AUC=0.745) or metabolomic (cross-validated AUC=0.820) analysis alone. Our ST:STUDY_SUMMARY findings may provide insights into the pathogenesis of ME/CFS and ME/CFS ST:STUDY_SUMMARY subtypes, and suggest pathways for the development of diagnostic and therapeutic ST:STUDY_SUMMARY strategies. ST:INSTITUTE University of California, Davis ST:DEPARTMENT Genome and Biomedical Sciences Facility ST:LABORATORY WCMC Metabolomics Core ST:LAST_NAME Fiehn ST:FIRST_NAME Oliver ST:ADDRESS 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, ST:ADDRESS CA 95616 ST:EMAIL ofiehn@ucdavis.edu ST:PHONE (530) 754-8258 ST:STUDY_COMMENTS Key: MECFS: 1 in this column indicates case, while 0 indicates control IBS: 1 in ST:STUDY_COMMENTS this column indicates the patient does have disease, 0 indicates free of IBS #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS 161024aOEsa48_1 ESP-0007_001 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=70; weight (lbs.)=154; bmi=22.09 SUBJECT_SAMPLE_FACTORS 161024aOEsa40_1 ESP-0020_002 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=70; weight (lbs.)=230; bmi=33 SUBJECT_SAMPLE_FACTORS 161024aOEsa35_1 ESP-0033_003 CaseStatus:Case | MECFS:1 | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=75; weight (lbs.)=252; bmi=31.49 SUBJECT_SAMPLE_FACTORS 161024aOEsa04_1 ESP-0046_004 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=61; weight (lbs.)=148; bmi=27.96 SUBJECT_SAMPLE_FACTORS 161025aOEsa26_1 ESP-0059_005 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=68; weight (lbs.)=140; bmi=21.28 SUBJECT_SAMPLE_FACTORS 161024aOEsa13_1 ESP-0072_006 CaseStatus:Case | MECFS:1 | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=76; weight (lbs.)=206; bmi=25.07 SUBJECT_SAMPLE_FACTORS 161024aOEsa29_1 ESP-0085_007 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=64; weight (lbs.)=175; bmi=30.04 SUBJECT_SAMPLE_FACTORS 161024aOEsa23_1 ESP-0098_008 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=58; weight (lbs.)=130; bmi=27.17 SUBJECT_SAMPLE_FACTORS 161024aOEsa14_1 ESP-0111_009 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=67; weight (lbs.)=165.4; bmi=25.9 SUBJECT_SAMPLE_FACTORS 161025aOEsa30_1 ESP-0124_010 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=65; weight (lbs.)=143; bmi=23.79 SUBJECT_SAMPLE_FACTORS 161025aOEsa02_1 ESP-0137_011 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=72; weight (lbs.)=147; bmi=19.93 SUBJECT_SAMPLE_FACTORS 161025aOEsa07_1 ESP-0150_012 CaseStatus:Control | MECFS:- | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=68; weight (lbs.)=158; bmi=24.02 SUBJECT_SAMPLE_FACTORS 161025aOEsa22_1 ESP-0163_013 CaseStatus:Control | MECFS:- | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=72; weight (lbs.)=178; bmi=24.14 SUBJECT_SAMPLE_FACTORS 161025aOEsa24_1 ESP-0176_014 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=67; weight (lbs.)=125; bmi=19.58 SUBJECT_SAMPLE_FACTORS 161025aOEsa23_1 ESP-0189_015 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=64; weight (lbs.)=200; bmi=34.33 SUBJECT_SAMPLE_FACTORS 161025aOEsa08_1 ESP-0202_016 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=62; weight (lbs.)=140; bmi=25.6 SUBJECT_SAMPLE_FACTORS 161024aOEsa12_1 ESP-0215_017 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=64; weight (lbs.)=150; bmi=25.74 SUBJECT_SAMPLE_FACTORS 161025aOEsa41_1 ESP-0228_018 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=64; weight (lbs.)=124.6; bmi=21.39 SUBJECT_SAMPLE_FACTORS 161025aOEsa37_1 ESP-0241_019 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=62.5; weight (lbs.)=180; bmi=32.39 SUBJECT_SAMPLE_FACTORS 161025aOEsa46_1 ESP-0254_020 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=64; weight (lbs.)=118; bmi=20.25 SUBJECT_SAMPLE_FACTORS 161025aOEsa12_1 ESP-0267_021 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=63; weight (lbs.)=156; bmi=27.63 SUBJECT_SAMPLE_FACTORS 161024aOEsa05_2 ESP-0280_022 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=69; weight (lbs.)=207; bmi=30.57 SUBJECT_SAMPLE_FACTORS 161025aOEsa04_1 ESP-0293_023 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=61; weight (lbs.)=160; bmi=30.23 SUBJECT_SAMPLE_FACTORS 161025aOEsa18_1 ESP-0306_024 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=67; weight (lbs.)=230; bmi=36.02 SUBJECT_SAMPLE_FACTORS 161024aOEsa42_1 ESP-0319_025 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=62; weight (lbs.)=120; bmi=21.95 SUBJECT_SAMPLE_FACTORS 161024aOEsa33_1 ESP-0332_026 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=68.5; weight (lbs.)=140.5; bmi=21.05 SUBJECT_SAMPLE_FACTORS 161024aOEsa45_1 ESP-0345_027 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=67; weight (lbs.)=145; bmi=22.71 SUBJECT_SAMPLE_FACTORS 161024aOEsa32_1 ESP-0358_028 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=65; weight (lbs.)=128.8; bmi=21.43 SUBJECT_SAMPLE_FACTORS 161025aOEsa09_2 ESP-0371_029 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=62; weight (lbs.)=231; bmi=42.25 SUBJECT_SAMPLE_FACTORS 161025aOEsa03_1 ESP-0384_030 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=65; weight (lbs.)=159; bmi=26.46 SUBJECT_SAMPLE_FACTORS 161025aOEsa11_1 ESP-0397_031 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=71; weight (lbs.)=149; bmi=20.78 SUBJECT_SAMPLE_FACTORS 161025aOEsa01_1 ESP-0410_032 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=60; weight (lbs.)=157.4; bmi=30.74 SUBJECT_SAMPLE_FACTORS 161024aOEsa24_1 ESP-0423_033 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=64; weight (lbs.)=166.4; bmi=28.56 SUBJECT_SAMPLE_FACTORS 161025aOEsa48_1 ESP-0436_034 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=63; weight (lbs.)=122; bmi=21.61 SUBJECT_SAMPLE_FACTORS 161024aOEsa34_1 ESP-0449_035 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=63; weight (lbs.)=170; bmi=30.11 SUBJECT_SAMPLE_FACTORS 161025aOEsa27_1 ESP-0462_036 CaseStatus:Case | MECFS:1 | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=68; weight (lbs.)=189; bmi=28.73 SUBJECT_SAMPLE_FACTORS 161025aOEsa21_1 ESP-0475_037 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=64; weight (lbs.)=142; bmi=24.37 SUBJECT_SAMPLE_FACTORS 161024aOEsa37_2 ESP-0488_038 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=63; weight (lbs.)=150; bmi=26.57 SUBJECT_SAMPLE_FACTORS 161024aOEsa11_1 ESP-0501_039 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=64; weight (lbs.)=160; bmi=27.46 SUBJECT_SAMPLE_FACTORS 161024aOEsa20_1 ESP-0514_040 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=65; weight (lbs.)=144; bmi=23.96 SUBJECT_SAMPLE_FACTORS 161025aOEsa43_1 ESP-0527_041 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=65; weight (lbs.)=120; bmi=19.97 SUBJECT_SAMPLE_FACTORS 161024aOEsa31_1 ESP-0540_042 CaseStatus:Control | MECFS:- | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=72; weight (lbs.)=160; bmi=21.7 SUBJECT_SAMPLE_FACTORS 161025aOEsa47_1 ESP-0553_043 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=64; weight (lbs.)=153; bmi=26.26 SUBJECT_SAMPLE_FACTORS 161025aOEsa32_1 ESP-0566_044 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=68; weight (lbs.)=168; bmi=25.54 SUBJECT_SAMPLE_FACTORS 161024aOEsa02_1 ESP-0579_045 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=65; weight (lbs.)=135; bmi=22.46 SUBJECT_SAMPLE_FACTORS 161024aOEsa49_1 ESP-0592_046 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=64; weight (lbs.)=150; bmi=25.74 SUBJECT_SAMPLE_FACTORS 161025aOEsa50_1 ESP-0605_047 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=61; weight (lbs.)=96.6; bmi=18.25 SUBJECT_SAMPLE_FACTORS 161024aOEsa03_1 ESP-0618_048 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=61; weight (lbs.)=120; bmi=22.67 SUBJECT_SAMPLE_FACTORS 161025aOEsa14_1 ESP-0631_049 CaseStatus:Control | MECFS:- | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=72; weight (lbs.)=194; bmi=26.31 SUBJECT_SAMPLE_FACTORS 161024aOEsa06_1 ESP-0644_050 CaseStatus:Case | MECFS:1 | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=67.5; weight (lbs.)=160; bmi=24.69 SUBJECT_SAMPLE_FACTORS 161024aOEsa39_1 ESP-0657_051 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=61; weight (lbs.)=137.8; bmi=26.03 SUBJECT_SAMPLE_FACTORS 161024aOEsa46_1 ESP-0670_052 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=63; weight (lbs.)=175.8; bmi=31.14 SUBJECT_SAMPLE_FACTORS 161024aOEsa15_1 ESP-0683_053 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=67; weight (lbs.)=125; bmi=19.58 SUBJECT_SAMPLE_FACTORS 161025aOEsa13_1 ESP-0696_054 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=67; weight (lbs.)=194.6; bmi=30.48 SUBJECT_SAMPLE_FACTORS 161024aOEsa19_1 ESP-0709_055 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=63; weight (lbs.)=132; bmi=23.38 SUBJECT_SAMPLE_FACTORS 161025aOEsa29_1 ESP-0722_056 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=66; weight (lbs.)=205; bmi=33.08 SUBJECT_SAMPLE_FACTORS 161024aOEsa16_1 ESP-0735_057 CaseStatus:Control | MECFS:- | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=71; weight (lbs.)=175; bmi=24.4 SUBJECT_SAMPLE_FACTORS 161024aOEsa47_1 ESP-0748_058 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=65; weight (lbs.)=130; bmi=21.63 SUBJECT_SAMPLE_FACTORS 161025aOEsa35_1 ESP-0761_059 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=58.8; weight (lbs.)=115; bmi=23.38 SUBJECT_SAMPLE_FACTORS 161025aOEsa17_1 ESP-0774_060 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=66; weight (lbs.)=138; bmi=22.27 SUBJECT_SAMPLE_FACTORS 161024aOEsa01_1 ESP-0787_061 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=64; weight (lbs.)=158; bmi=27.12 SUBJECT_SAMPLE_FACTORS 161024aOEsa09_1 ESP-0800_062 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=66; weight (lbs.)=138; bmi=22.27 SUBJECT_SAMPLE_FACTORS 161025aOEsa45_1 ESP-0813_063 CaseStatus:Case | MECFS:1 | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=70; weight (lbs.)=185; bmi=26.54 SUBJECT_SAMPLE_FACTORS 161025aOEsa31_1 ESP-0826_064 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Male; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=67; weight (lbs.)=195; bmi=30.54 SUBJECT_SAMPLE_FACTORS 161024aOEsa41_1 ESP-0839_065 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=72; weight (lbs.)=179; bmi=24.27 SUBJECT_SAMPLE_FACTORS 161024aOEsa10_1 ESP-0852_066 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=68; weight (lbs.)=217; bmi=32.99 SUBJECT_SAMPLE_FACTORS 161024aOEsa27_1 ESP-0865_067 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=65; weight (lbs.)=124; bmi=20.63 SUBJECT_SAMPLE_FACTORS 161024aOEsa30_1 ESP-0878_068 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=67; weight (lbs.)=192.2; bmi=30.1 SUBJECT_SAMPLE_FACTORS 161025aOEsa10_2 ESP-0891_069 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=63.5; weight (lbs.)=219; bmi=38.18 SUBJECT_SAMPLE_FACTORS 161024aOEsa50_1 ESP-0904_070 CaseStatus:Case | MECFS:1 | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=68; weight (lbs.)=164; bmi=24.93 SUBJECT_SAMPLE_FACTORS 161025aOEsa40_1 ESP-0917_071 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=65; weight (lbs.)=178; bmi=29.62 SUBJECT_SAMPLE_FACTORS 161025aOEsa36_1 ESP-0930_072 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=66; weight (lbs.)=140; bmi=22.59 SUBJECT_SAMPLE_FACTORS 161025aOEsa16_1 ESP-0943_073 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=68; weight (lbs.)=150; bmi=22.8 SUBJECT_SAMPLE_FACTORS 161025aOEsa20_1 ESP-0956_074 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=61; weight (lbs.)=146; bmi=27.58 SUBJECT_SAMPLE_FACTORS 161024aOEsa26_1 ESP-0969_075 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=62; weight (lbs.)=136; bmi=24.87 SUBJECT_SAMPLE_FACTORS 161024aOEsa17_1 ESP-0982_076 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=66; weight (lbs.)=154; bmi=24.85 SUBJECT_SAMPLE_FACTORS 161025aOEsa49_1 ESP-0995_077 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=67.5; weight (lbs.)=140; bmi=21.6 SUBJECT_SAMPLE_FACTORS 161025aOEsa05_1 ESP-1008_078 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=60; weight (lbs.)=140; bmi=27.34 SUBJECT_SAMPLE_FACTORS 161024aOEsa08_1 ESP-1021_079 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=64; weight (lbs.)=135; bmi=23.17 SUBJECT_SAMPLE_FACTORS 161024aOEsa18_1 ESP-1034_080 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=64; weight (lbs.)=218; bmi=37.42 SUBJECT_SAMPLE_FACTORS 161025aOEsa34_1 ESP-1047_081 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=62; weight (lbs.)=115; bmi=21.03 SUBJECT_SAMPLE_FACTORS 161024aOEsa21_1 ESP-1060_082 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=64; weight (lbs.)=130; bmi=22.31 SUBJECT_SAMPLE_FACTORS 161025aOEsa28_1 ESP-1073_083 CaseStatus:Control | MECFS:- | IBS:- SEX=Male; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=65; weight (lbs.)=180; bmi=29.95 SUBJECT_SAMPLE_FACTORS 161025aOEsa15_1 ESP-1086_084 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=68; weight (lbs.)=143; bmi=21.74 SUBJECT_SAMPLE_FACTORS 161025aOEsa33_1 ESP-1099_085 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=61; weight (lbs.)=120; bmi=22.67 SUBJECT_SAMPLE_FACTORS 161024aOEsa07_1 ESP-1112_086 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=64; weight (lbs.)=149; bmi=25.57 SUBJECT_SAMPLE_FACTORS 161025aOEsa44_1 ESP-1125_087 CaseStatus:Case | MECFS:1 | IBS:1 SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=69; weight (lbs.)=234; bmi=34.55 SUBJECT_SAMPLE_FACTORS 161024aOEsa44_1 ESP-1138_088 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=70; weight (lbs.)=208; bmi=29.84 SUBJECT_SAMPLE_FACTORS 161025aOEsa42_1 ESP-1151_089 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=65.5; weight (lbs.)=150.2; bmi=24.61 SUBJECT_SAMPLE_FACTORS 161025aOEsa06_1 ESP-1164_090 CaseStatus:Case | MECFS:1 | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=65; weight (lbs.)=177; bmi=29.45 SUBJECT_SAMPLE_FACTORS 161024aOEsa43_1 ESP-1177_091 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Overweight.Obese; height (in.)=63; weight (lbs.)=162.6; bmi=28.8 SUBJECT_SAMPLE_FACTORS 161025aOEsa19_1 ESP-1190_092 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=63; weight (lbs.)=130; bmi=23.03 SUBJECT_SAMPLE_FACTORS 161025aOEsa39_1 ESP-1203_093 CaseStatus:Case | MECFS:1 | IBS:- SEX=Male; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=71; weight (lbs.)=215; bmi=29.98 SUBJECT_SAMPLE_FACTORS 161025aOEsa25_1 ESP-1216_094 CaseStatus:Control | MECFS:- | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=70; weight (lbs.)=172; bmi=24.68 SUBJECT_SAMPLE_FACTORS 161024aOEsa25_1 ESP-1229_095 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=67; weight (lbs.)=139; bmi=21.77 SUBJECT_SAMPLE_FACTORS 161024aOEsa28_1 ESP-1255_096 CaseStatus:Case | MECFS:1 | IBS:- SEX=Male; AGE_DICH=over50; BMI_DICH=Underweight.Normal; height (in.)=70; weight (lbs.)=168; bmi=24.1 SUBJECT_SAMPLE_FACTORS 161024aOEsa38_1 ESP-1268_097 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=66.6; weight (lbs.)=170; bmi=26.94 SUBJECT_SAMPLE_FACTORS 161024aOEsa22_1 ESP-1281_098 CaseStatus:Control | MECFS:- | IBS:- SEX=Male; AGE_DICH=20_50; BMI_DICH=Overweight.Obese; height (in.)=68; weight (lbs.)=206; bmi=31.32 SUBJECT_SAMPLE_FACTORS 161025aOEsa38_1 ESP-1294_099 CaseStatus:Control | MECFS:- | IBS:- SEX=Female; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=66; weight (lbs.)=120; bmi=19.37 SUBJECT_SAMPLE_FACTORS 161024aOEsa36_1 ESP-1307_100 CaseStatus:Control | MECFS:- | IBS:- SEX=Male; AGE_DICH=20_50; BMI_DICH=Underweight.Normal; height (in.)=76; weight (lbs.)=185; bmi=22.52 SUBJECT_SAMPLE_FACTORS 161026aOEsa04_1 Pool 1 CaseStatus:- | MECFS:- | IBS:- SEX=-; AGE_DICH=-; BMI_DICH=-; height (in.)=-; weight (lbs.)=-; bmi=- SUBJECT_SAMPLE_FACTORS 161026aOEsa03_1 Pool 2 CaseStatus:- | MECFS:- | IBS:- SEX=-; AGE_DICH=-; BMI_DICH=-; height (in.)=-; weight (lbs.)=-; bmi=- SUBJECT_SAMPLE_FACTORS 161026aOEsa02_1 Pool 3 CaseStatus:- | MECFS:- | IBS:- SEX=-; AGE_DICH=-; BMI_DICH=-; height (in.)=-; weight (lbs.)=-; bmi=- SUBJECT_SAMPLE_FACTORS 161026aOEsa01_1 Pool 4 CaseStatus:- | MECFS:- | IBS:- SEX=-; AGE_DICH=-; BMI_DICH=-; height (in.)=-; weight (lbs.)=-; bmi=- SUBJECT_SAMPLE_FACTORS 161026aOEsa10_2 Pool 5 CaseStatus:- | MECFS:- | IBS:- SEX=-; AGE_DICH=-; BMI_DICH=-; height (in.)=-; weight (lbs.)=-; bmi=- SUBJECT_SAMPLE_FACTORS 161026aOEsa05_1 Pool 6 CaseStatus:- | MECFS:- | IBS:- SEX=-; AGE_DICH=-; BMI_DICH=-; height (in.)=-; weight (lbs.)=-; bmi=- SUBJECT_SAMPLE_FACTORS 161026aOEsa08_1 Pool 7 CaseStatus:- | MECFS:- | IBS:- SEX=-; AGE_DICH=-; BMI_DICH=-; height (in.)=-; weight (lbs.)=-; bmi=- SUBJECT_SAMPLE_FACTORS 161026aOEsa07_1 Pool 8 CaseStatus:- | MECFS:- | IBS:- SEX=-; AGE_DICH=-; BMI_DICH=-; height (in.)=-; weight (lbs.)=-; bmi=- SUBJECT_SAMPLE_FACTORS 161026aOEsa06_2 Pool 9 CaseStatus:- | MECFS:- | IBS:- SEX=-; AGE_DICH=-; BMI_DICH=-; height (in.)=-; weight (lbs.)=-; bmi=- SUBJECT_SAMPLE_FACTORS 161026aOEsa09_1 Pool 10 CaseStatus:- | MECFS:- | IBS:- SEX=-; AGE_DICH=-; BMI_DICH=-; height (in.)=-; weight (lbs.)=-; bmi=- #COLLECTION CO:COLLECTION_SUMMARY Subjects included 50 cases and 50 controls from the Chronic Fatigue Initiative CO:COLLECTION_SUMMARY (CFI) Cohort (28) recruited at 4 sites across the US who met the 1994 CDC Fukuda CO:COLLECTION_SUMMARY and/or Canadian consensus criteria for ME/CFS. CO:SAMPLE_TYPE Blood CO:BLOOD_SERUM_OR_PLASMA Plasma #TREATMENT TR:TREATMENT_SUMMARY Subjects included 50 cases and 50 controls from the Chronic Fatigue Initiative TR:TREATMENT_SUMMARY (CFI) Cohort (28) recruited at 4 sites across the US who met the 1994 CDC Fukuda TR:TREATMENT_SUMMARY and/or Canadian consensus criteria for ME/CFS. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY 1.Thaw each 20 μL plasma aliquot at room temperature (see Aliquoting TEDDY SP:SAMPLEPREP_SUMMARY samples SOP). Once thawed (~10min) place liquid plasma samples on ice. 2. Add SP:SAMPLEPREP_SUMMARY 225 μL cold “MeOH with QC mix” (see SOP “QC mix for LC-MS lipid SP:SAMPLEPREP_SUMMARY analysis”). Keep MeOH on ice during extraction 3. Vortex each sample for 10s, SP:SAMPLEPREP_SUMMARY keeping the rest on ice during all the extraction. 4. Add 750 μL of cold MTBE SP:SAMPLEPREP_SUMMARY with 22:1 CE, keep MTBE on ice during extraction 5. Vortex for 10s 6. Shake for SP:SAMPLEPREP_SUMMARY 6min at 4°C in the orbital mixer. 7. Add 188 μL room temperature LC/MS grade SP:SAMPLEPREP_SUMMARY water. 8. Vortex for 20 s 9. Centrifuge for 2 min @ 14,000 rcf (12300 rpm) 10. SP:SAMPLEPREP_SUMMARY Remove supernatant, splitting into two aliquots of 350 μL, keeping one at SP:SAMPLEPREP_SUMMARY –20°C for backup 11. Dry samples to complete dryness in the speed vacuum SP:SAMPLEPREP_SUMMARY concentration system SP:SAMPLEPREP_PROTOCOL_FILENAME SP_SOP_Extraction_of_plasma.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Leco Pegasus III GC CH:COLUMN_NAME Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um) CH:COLUMN_NAME 0.25 μm film made of 95% dimethyl/5%diphenylpolysiloxane) CH:FLOW_RATE 1 ml/min CH:COLUMN_TEMPERATURE 50 - 330°C CH:COLUMN_PRESSURE 7.7 PSI (initial condition) CH:INJECTION_TEMPERATURE 50°C ramped to 250°C by 12°C/s CH:SAMPLE_INJECTION 0.5µl CH:OVEN_TEMPERATURE 50°C for 1 min, then ramped at 20°C/min to 330°C, held constant for 5 min CH:TRANSFERLINE_TEMPERATURE 230°C CH:WASHING_BUFFER Ethyl Acetate CH:SAMPLE_LOOP_SIZE 30 m length x 0.25 mm internal diameter CH:RANDOMIZATION_ORDER Excel generated #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Leco Pegasus III GC TOF MS:INSTRUMENT_TYPE GC-TOF MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:ION_SOURCE_TEMPERATURE 250°C MS:IONIZATION_ENERGY 70eV MS:MASS_ACCURACY Nominal MS:SOURCE_TEMPERATURE 250°C MS:SCAN_RANGE_MOVERZ 85-500 MS:SCANNING_CYCLE 17 Hz MS:SCANNING_RANGE 80-500 Da MS:SKIMMER_VOLTAGE 1850 MS:MS_RESULTS_FILE ST000813_AN001286_Results.txt UNITS:Counts #END