#METABOLOMICS WORKBENCH araskind_20170714_123806 DATATRACK_ID:1135 STUDY_ID:ST000820 ANALYSIS_ID:AN001303 PROJECT_ID:PR000584 VERSION 1 CREATED_ON August 1, 2017, 3:36 pm #PROJECT PR:PROJECT_TITLE 2HG prodution in neurospheres from IDH1 mouse glioma model PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Evaluation of 2HG and a-ketoglutarate in tumor neurospheres (NS) genertated from PR:PROJECT_SUMMARY the mice brain tumors haboring specific genetic lesions: NRAS overexpression, PR:PROJECT_SUMMARY p53 knockdown plus or minus IDH1 mutated and ATRX knockdown. PR:INSTITUTE University of Michigan PR:DEPARTMENT Neurosurgery PR:LABORATORY Castro Lab PR:LAST_NAME Castro PR:FIRST_NAME Maria PR:ADDRESS Ann Arbor, MI PR:EMAIL mariacas@umich.edu PR:PHONE 734-764-0850 #STUDY ST:STUDY_TITLE 2 hydroxyglutarate prodution in neurospheres from IDH1 mouse glioma model #2 ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Evaluation of 2HG and a-ketoglutarate in tumor neurospheres (NS) genertated from ST:STUDY_SUMMARY the mice brain tumors haboring specific genetic lesions: NRAS overexpression, ST:STUDY_SUMMARY p53 knockdown plus or minus IDH1 mutated and ATRX knockdown. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 4 #SUBJECT SU:SUBJECT_TYPE MOUSE SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0016367 S00026214 IDH1_MUTATION:NO SUBJECT_SAMPLE_FACTORS SU0016367 S00026215 IDH1_MUTATION:NO SUBJECT_SAMPLE_FACTORS SU0016368 S00026216 IDH1_MUTATION:IDH1-R132H SUBJECT_SAMPLE_FACTORS SU0016368 S00026217 IDH1_MUTATION:IDH1-R132H SUBJECT_SAMPLE_FACTORS SU0016369 S00026218 IDH1_MUTATION:IDH1-R132H SUBJECT_SAMPLE_FACTORS SU0016369 S00026219 IDH1_MUTATION:IDH1-R132H SUBJECT_SAMPLE_FACTORS SU0016370 S00026220 IDH1_MUTATION:IDH1-R132H SUBJECT_SAMPLE_FACTORS SU0016370 S00026221 IDH1_MUTATION:IDH1-R132H #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A037-2HG cells updated-20160721.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A037-2HG cells updated-20160721.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6490A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples S00026214 S00026215 S00026216 S00026217 S00026218 S00026219 S00026220 S00026221 Factors IDH1_MUTATION:NO IDH1_MUTATION:NO IDH1_MUTATION:IDH1-R132H IDH1_MUTATION:IDH1-R132H IDH1_MUTATION:IDH1-R132H IDH1_MUTATION:IDH1-R132H IDH1_MUTATION:IDH1-R132H IDH1_MUTATION:IDH1-R132H (2R)-2-HYDROXYPENTANEDIOIC ACID .25363048 .342549776 11.06987297 10.53820051 8.167143088 10.30796916 1.780790067 2.87467369 (2S)-2-HYDROXYPENTANEDIOIC ACID .358184959 .340863541 0 0 0 0 .404508486 .538581489 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type (2R)-2-HYDROXYPENTANEDIOIC ACID 439391 (2S)-2-HYDROXYPENTANEDIOIC ACID 439939 METABOLITES_END #END