#METABOLOMICS WORKBENCH araskind_20170714_125110 DATATRACK_ID:1142 STUDY_ID:ST000824 ANALYSIS_ID:AN001310 VERSION 1 CREATED_ON 12-15-2022 #PROJECT PR:PROJECT_TITLE Production of 2HG of human glioma cells PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Evaluate the concentration of 2HG in human glioma cells harboring IDH1-R132H PR:PROJECT_SUMMARY mutation PR:INSTITUTE University of Michigan PR:DEPARTMENT Neurosurgery PR:LABORATORY Castro Lab PR:LAST_NAME Castro PR:FIRST_NAME Maria PR:ADDRESS Ann Arbor, MI PR:EMAIL mariacas@umich.edu PR:PHONE 734-764-0850 PR:DOI http://dx.doi.org/10.21228/M8XD4X #STUDY ST:STUDY_TITLE 2HG concentration in human IDH1m cells ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY SJGBM2 and MGG8 glioma cells were stable transfected with IDH1-R132H mutated. ST:STUDY_SUMMARY The expression of IDH1-R132H was confirmed by Western Blot assay. The aim of ST:STUDY_SUMMARY this project is analyze the production of 2HG in the stable transfected cells ST:STUDY_SUMMARY (SJGBM-IDH1m and MGG8-IDH1m) compared with the control group WT. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:SUBMIT_DATE 2017-07-14 #SUBJECT SU:SUBJECT_TYPE HUMAN SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:SPECIES_GROUP Human #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0018091 S00028188 Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028189 Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028190 Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028200 Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES SUBJECT_SAMPLE_FACTORS SU0018091 S00028201 Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES SUBJECT_SAMPLE_FACTORS SU0018091 S00028202 Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES SUBJECT_SAMPLE_FACTORS SU0018091 S00028185 Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028186 Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028187 Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028197 Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES SUBJECT_SAMPLE_FACTORS SU0018091 S00028198 Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES SUBJECT_SAMPLE_FACTORS SU0018091 S00028199 Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES SUBJECT_SAMPLE_FACTORS SU0018091 S00028182 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028183 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028184 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028194 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES SUBJECT_SAMPLE_FACTORS SU0018091 S00028195 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES SUBJECT_SAMPLE_FACTORS SU0018091 S00028196 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES SUBJECT_SAMPLE_FACTORS SU0018091 S00028179 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028180 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028181 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO SUBJECT_SAMPLE_FACTORS SU0018091 S00028191 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES SUBJECT_SAMPLE_FACTORS SU0018091 S00028192 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES SUBJECT_SAMPLE_FACTORS SU0018091 S00028193 Cell line:SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Glioma cells #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A037-2HG_cells_updated-20160721.pdf #CHROMATOGRAPHY CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm,3um) CH:SOLVENT_A 100% water; 2mM ammonium formate, pH 3.3-4.01,adjust with formic acid CH:SOLVENT_B 100% acetonitrile; 0.1% formic acid CH:CHROMATOGRAPHY_TYPE HILIC #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A037-2HG cells updated-20160721.pdf #MS MS:INSTRUMENT_NAME Agilent 6490A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM/ng protein MS_METABOLITE_DATA_START Samples S00028188 S00028189 S00028190 S00028200 S00028201 S00028202 S00028185 S00028186 S00028187 S00028197 S00028198 S00028199 S00028182 S00028183 S00028184 S00028194 S00028195 S00028196 S00028180 S00028181 S00028191 S00028192 S00028193 Factors Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES Cell line:MGG8 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES Cell line:MGG8 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:NO Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES Cell line:SJGBM2 | IDH1-R132H EXPRESSION:NO | IDH1-R132H INHIBITOR:YES Cell line:SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO Cell line:SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:NO Cell line:SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES Cell line:SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES Cell line:SJGBM2 | IDH1-R132H EXPRESSION:YES | IDH1-R132H INHIBITOR:YES 6-PHOSPHOGLUCONIC ACID 0.1530 0.0620 0.1188 0.1467 0.0837 0.0249 0.0229 0.0170 0.0661 0.0113 0.0112 0.0692 0.0191 0.0175 0.2432 0.0418 0.0163 0.0199 0.0228 0.0194 0.0535 0.1635 0.0335 ACETOACETYL-COA 0.0030 0.0025 0.0037 0.0032 0.0030 0.0025 0.0027 0.0025 0.0050 0.0021 0.0026 0.0036 0.0032 0.0038 0.0037 0.0034 0.0042 0.0041 0.0016 0.0019 0.0018 0.0021 0.0023 ADENOSINE 5^-DIPHOSPHATE 0.1169 0.0700 0.0695 0.1877 0.0544 0.0312 0.0275 0.0230 0.0727 0.0235 0.0266 0.0700 0.0210 0.0316 0.0743 0.0478 0.0318 0.0250 0.0193 0.0245 0.0317 0.0538 0.0396 ADENOSINE 5^-MONOPHOSPHATE 0.0234 0.0212 0.0336 0.0481 0.0135 0.0034 0.0036 0.0023 0.0137 0.0024 0.0049 0.0113 0.0114 0.0100 0.0536 0.0140 0.0079 0.0133 0.0025 0.0024 0.0042 0.0043 0.0043 ADENOSINE TRIPHOSPHATE 0.2892 0.1048 0.1606 0.3043 0.1129 0.2120 0.0669 0.0551 0.5684 0.1650 0.1181 0.3407 0.0331 0.0556 0.0641 0.1471 0.0820 0.0330 0.0922 0.1724 0.1304 0.2754 0.0828 BETA-NAD+ 0.0227 0.0178 0.0140 0.0515 0.0089 0.0108 0.0109 0.0125 0.0145 0.0086 0.0068 0.0212 0.0040 0.0045 0.0153 0.0080 0.0046 0.0035 0.0069 0.0089 0.0066 0.0144 0.0099 CITRIC ACID 0.1980 0.0559 0.1549 0.2336 0.1148 0.0899 0.0524 0.0882 0.1513 0.0305 0.0319 0.2719 0.0413 0.0344 0.2648 0.0586 0.0349 0.0327 0.0896 0.1128 0.1011 0.4263 0.1123 COENZYME II 0.0032 0.0023 0.0042 0.0038 0.0028 0.0025 0.0027 0.0026 0.0048 0.0023 0.0024 0.0031 0.0027 0.0032 0.0055 0.0033 0.0034 0.0033 0.0017 0.0021 0.0017 0.0032 0.0028 D-ERYTHROSE 4-PHOSPHATE 0.3388 0.0948 0.1186 0.3015 0.1556 0.0351 0.0466 0.0341 0.1045 0.0254 0.0218 0.1160 0.0159 0.0425 0.2480 0.0516 0.0165 0.0246 0.0245 0.0311 0.0666 0.1357 0.0689 D-FRUCTOSE_ 1_6-BIS(DIHYDROGEN PHOSPHATE) 0.0859 0.0157 0.1084 0.1289 0.0565 0.1970 0.2609 0.3100 1.4622 0.2104 0.2963 0.7049 0.2238 0.0427 0.1029 0.0896 0.2703 0.0127 0.0853 0.1605 0.1036 0.3149 0.8833 FAD 0.0013 0.0005 0.0015 0.0029 0.0002 0.0007 0.0003 0.0004 0.0019 0.0007 0.0002 0.0022 0.0022 0.0002 0.0006 0.0010 0.0007 0.0025 0.0020 GLYCERATE 3-PHOSPHATE 0.0425 0.0138 0.0516 0.0412 0.0296 0.0620 0.0326 0.0281 0.4563 0.0340 0.0319 0.2333 0.0553 0.0335 0.0686 0.0382 0.0755 0.0166 0.0187 0.0366 0.0235 0.0657 0.1855 MALIC ACID 0.0593 0.0454 0.0821 0.0525 0.0331 0.0487 0.0248 0.0238 0.0324 0.0294 0.1737 0.0675 0.0540 0.0254 0.0556 0.0793 0.0443 NADH 0.0220 0.0142 0.0772 0.0967 0.0142 0.0790 0.0285 0.0453 0.0684 0.0794 0.0181 0.1785 0.0222 0.0143 0.0027 0.0234 0.0271 0.0317 0.0126 0.0403 0.1183 NADP-REDUCED 0.0210 0.0059 0.0174 0.0301 0.0088 0.0080 0.0060 0.0095 0.0168 0.0076 0.0040 0.0278 0.0017 0.0033 0.0022 0.0032 0.0022 0.0025 0.0043 0.0082 0.0059 PHOSPHOENOLPYRUVATE 0.0064 0.0039 0.0042 0.0068 0.0052 0.0052 0.0029 0.0027 0.0109 0.0030 0.0033 0.0100 0.0039 0.0040 0.0044 0.0036 0.0051 0.0041 0.0022 0.0021 0.0024 0.0038 0.0082 RIBOSE PHOSPHATE 0.0442 0.0305 0.0925 0.0817 0.0152 0.0467 0.0390 0.0390 0.1415 0.0305 0.0267 0.1417 0.0545 0.1217 0.0329 0.0410 0.0181 0.0320 0.0153 0.0686 0.1203 ROBISON ESTER 0.4547 0.1242 0.1559 0.3710 0.2198 0.0516 0.0667 0.0541 0.1236 0.0345 0.0379 0.1561 0.0327 0.0662 0.2857 0.0682 0.0396 0.0443 0.0359 0.0457 0.0946 0.1771 0.0896 SEDOHEPTULOSE 7-PHOSPHATE 0.0300 0.0194 0.0178 0.0512 0.0165 0.0079 0.0063 0.0069 0.0192 0.0056 0.0045 0.0168 0.0043 0.0016 0.0188 0.0070 0.0043 0.0023 0.0036 0.0053 0.0068 0.0146 0.0156 SUCROSE 0.0466 0.0306 0.0263 0.0956 0.0252 0.0213 0.0129 0.0137 0.0670 0.0157 0.0163 0.0565 0.0156 0.0118 0.0468 0.0247 0.0189 0.0133 0.0194 0.0302 0.0190 0.0547 0.0445 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant 6-PHOSPHOGLUCONIC ACID 91493 ACETOACETYL-COA 92153 ADENOSINE 5'-DIPHOSPHATE 6022 ADENOSINE 5'-MONOPHOSPHATE 6083 ADENOSINE TRIPHOSPHATE 5957 BETA-NAD+ 15938971 CITRIC ACID 311 COENZYME II 15938972 D-ERYTHROSE 4-PHOSPHATE 122357 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 21125049 FAD 46906035 GLYCERATE 3-PHOSPHATE 724 MALIC ACID 525 NADH 439153 NADP-REDUCED 22833512 PHOSPHOENOLPYRUVATE 1005 RIBOSE PHOSPHATE 77982 ROBISON ESTER 5958 SEDOHEPTULOSE 7-PHOSPHATE 165007 SUCROSE 5988 METABOLITES_END #END