#METABOLOMICS WORKBENCH araskind_20170802_130550 DATATRACK_ID:1174 STUDY_ID:ST000827 ANALYSIS_ID:AN001315 PROJECT_ID:PR000589 VERSION 1 CREATED_ON August 7, 2017, 3:27 pm #PROJECT PR:PROJECT_TITLE Metabolic regulation by PARP-1 in myofibroblast differentiation and fibrosis PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Analysis of wild type lung tissue after saline or bleomycin treatment PR:INSTITUTE University of Michigan PR:DEPARTMENT Deaprtment of Pathology PR:LABORATORY Sem H. Phan PR:LAST_NAME Hu PR:FIRST_NAME Biao PR:ADDRESS Ann Arbor, MI PR:EMAIL biaohu@med.umich.edu PR:PHONE 734-7635731 #STUDY ST:STUDY_TITLE Metabolic analysis of Normal Mouse Lung Fiboblasts with/without TGFbeta ST:STUDY_TITLE treatment ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Glycolysis/TCA/Nucleotide analysis (especially interested in ST:STUDY_SUMMARY alpha-ketoglutarate) and NAD+ and related metabolite analysis for parp1 ko and ST:STUDY_SUMMARY parp1 wild type lung tissue after saline or bleomycin treatment ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 30 #SUBJECT SU:SUBJECT_TYPE MOUSE SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0003980 S00014554 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003981 S00014555 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003982 S00014556 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003983 S00014557 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0003984 S00014558 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0003985 S00014559 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0003986 S00014560 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003987 S00014561 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003988 S00014562 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003989 S00014563 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0003990 S00014564 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0003991 S00014565 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0003992 S00014566 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003993 S00014567 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003994 S00014568 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0003995 S00014569 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0003996 S00014570 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003997 S00014571 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003998 S00014572 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0003999 S00014573 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0004000 S00014574 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0004001 S00014575 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0004002 S00014576 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0004003 S00014577 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0004004 S00014578 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0004005 S00014579 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0004006 S00014580 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0004007 S00014581 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0004008 S00014582 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0004009 S00014583 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A010 - Ceramides.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Phenyl CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Pinnacle DB Biphenyl, 140A, 1.9mkm, 2.1mm X 50mm (Restek) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A010 - Ceramides.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6490A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS counts MS_METABOLITE_DATA_START Samples S00014554 S00014555 S00014556 S00014557 S00014558 S00014559 S00014560 S00014561 S00014562 S00014563 S00014564 S00014565 S00014566 S00014567 S00014568 S00014569 S00014570 S00014571 S00014572 S00014573 S00014574 S00014575 S00014576 S00014577 S00014578 S00014579 S00014580 S00014581 S00014582 S00014583 Factors Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes N-ICOSANOYLSPHINGOSINE 187726.2082 257996.5179 181075.134 119582.4733 133817.6561 81470.29751 181036.3685 209456.6369 320488.7738 132285.6415 131067.9974 164374.0507 222628.9276 310990.8647 197506.5612 103793.5756 506075.8974 367551.85 110971.7491 86491.02575 125452.0005 288947.7398 453491.4602 373769.5684 142085.6713 177898.5123 194922.2093 311762.3714 380515.7367 247455.1244 C22 CER 483038.069 583350.2541 355268.2794 230914.8352 306199.0198 224112.6849 657930.2302 504988.3198 887236.8942 220528.6348 230607.6113 290510.7538 456306.0274 908580.9516 448792.0912 303831.9649 1031293.125 884207.5029 264235.3273 175261.8318 252599.7688 663291.0877 866876.9734 714586.1185 331863.4573 251959.1613 334203.8306 711555.3195 644009.2631 687307.0188 C24:1 CER 1115337.067 1293227.6 803000.8996 1069697.973 983074.3611 724963.4276 1150454.036 1011958.89 1650140.043 831289.1932 871493.1217 1011155.527 890437.2805 1556087.736 1478464.951 1292919.823 2178300.24 1908131.013 979856.0874 794109.8388 895446.4592 1403065.76 1808533.639 1647473.141 1301576.557 1316577.435 1191452.575 1447581.604 1368437.153 1318523.472 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type N-ICOSANOYLSPHINGOSINE 5283566 C22 CER 5283567 C24:1 CER 5283568 METABOLITES_END #END