#METABOLOMICS WORKBENCH araskind_20170802_130550 DATATRACK_ID:1174 STUDY_ID:ST000827 ANALYSIS_ID:AN001316 PROJECT_ID:PR000589 VERSION 1 CREATED_ON August 7, 2017, 3:27 pm #PROJECT PR:PROJECT_TITLE Metabolic regulation by PARP-1 in myofibroblast differentiation and fibrosis PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Analysis of wild type lung tissue after saline or bleomycin treatment PR:INSTITUTE University of Michigan PR:DEPARTMENT Deaprtment of Pathology PR:LABORATORY Sem H. Phan PR:LAST_NAME Hu PR:FIRST_NAME Biao PR:ADDRESS Ann Arbor, MI PR:EMAIL biaohu@med.umich.edu PR:PHONE 734-7635731 #STUDY ST:STUDY_TITLE Metabolic analysis of Normal Mouse Lung Fiboblasts with/without TGFbeta ST:STUDY_TITLE treatment ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Glycolysis/TCA/Nucleotide analysis (especially interested in ST:STUDY_SUMMARY alpha-ketoglutarate) and NAD+ and related metabolite analysis for parp1 ko and ST:STUDY_SUMMARY parp1 wild type lung tissue after saline or bleomycin treatment ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 30 #SUBJECT SU:SUBJECT_TYPE MOUSE SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0003980 S00014554 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003981 S00014555 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003982 S00014556 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003983 S00014557 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0003984 S00014558 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0003985 S00014559 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0003986 S00014560 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003987 S00014561 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003988 S00014562 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003989 S00014563 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0003990 S00014564 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0003991 S00014565 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0003992 S00014566 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003993 S00014567 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003994 S00014568 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0003995 S00014569 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0003996 S00014570 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003997 S00014571 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0003998 S00014572 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0003999 S00014573 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0004000 S00014574 Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no SUBJECT_SAMPLE_FACTORS SU0004001 S00014575 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0004002 S00014576 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0004003 S00014577 Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes SUBJECT_SAMPLE_FACTORS SU0004004 S00014578 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0004005 S00014579 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0004006 S00014580 Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no SUBJECT_SAMPLE_FACTORS SU0004007 S00014581 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0004008 S00014582 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes SUBJECT_SAMPLE_FACTORS SU0004009 S00014583 Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A010 - Ceramides.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Phenyl CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Pinnacle DB Biphenyl, 140A, 1.9mkm, 2.1mm X 50mm (Restek) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A010 - Ceramides.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6490A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS ng/mg MS_METABOLITE_DATA_START Samples S00014554 S00014555 S00014556 S00014557 S00014558 S00014559 S00014560 S00014561 S00014562 S00014563 S00014564 S00014565 S00014566 S00014567 S00014568 S00014569 S00014570 S00014571 S00014572 S00014573 S00014574 S00014575 S00014576 S00014577 S00014578 S00014579 S00014580 S00014581 S00014582 S00014583 Factors Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:yes | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:no Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:no | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:yes Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:yes | Parp1 KO:yes | Parp1 WT (129S Background):no | Saline:no Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes Bleomycin:no | Parp1 KO:no | Parp1 WT (129S Background):yes | Saline:yes C14 CER 4.870631563 6.409223317 2.368004292 2.236688703 .340662321 3.660264842 5.964877314 4.578680604 10.84101496 1.268030255 1.597490906 2.125354344 3.512083989 8.055970241 2.208153178 4.705562861 15.21943391 12.13839689 2.219828537 .372543919 1.602381332 9.741891914 10.64649284 7.552983943 5.663100822 4.153119098 7.924634002 8.408115935 3.156288667 6.526388554 N-(OLEOYL)-CERAMIDE 12.10998476 21.36554143 9.469688574 7.585676576 5.031747405 16.77068054 13.65580571 11.56791985 20.89738994 6.391097495 12.13280734 9.197000138 7.053164426 17.35629111 7.256699043 16.29964236 29.34923448 26.79475303 12.20462969 6.142393355 6.93930832 20.72669341 22.28501717 19.91020906 16.20818866 10.71025268 13.39497781 16.84744559 9.806828884 18.29494578 N-PALMITOYLSPHINGOSINE 701.0611432 973.5663592 453.2647235 326.8010481 143.4809212 622.2838579 765.5192732 672.2072459 1176.712722 255.195869 287.8832213 317.6015162 464.8610583 1134.199893 272.6366978 607.1488745 1787.725782 1673.393349 351.1547258 152.619319 266.8630189 1298.874305 1279.559348 1017.915718 511.8555612 477.9647156 739.7600113 1107.329604 491.0329699 865.334808 N-STEAROYLSPHINGOSINE 51.61256825 64.26226951 40.78364429 16.77680617 7.979776314 41.46667015 71.49862373 55.30364437 102.0980671 14.39757251 17.26711861 23.46824141 34.31367523 95.93127503 19.24916249 47.89374342 129.8439766 136.2266713 21.33190361 7.106598751 16.84673003 93.45575055 99.71198806 70.71331147 34.41142968 26.80321523 50.46849007 69.75100479 30.68369042 78.13484451 N-ICOSANOYLSPHINGOSINE 174.3426167 255.7336918 139.6546282 62.20027444 30.71824372 109.1202363 151.3360777 170.8348307 265.8896638 53.72947796 57.46707467 69.71788676 138.2518386 284.5449537 60.1601222 82.18642729 493.4462211 439.1755741 59.38204138 23.74646226 54.95190095 368.2328801 414.9148607 323.5935989 61.72632185 94.15969817 161.1328646 320.5072369 156.0354536 230.290402 C24 CER 42.83291757 58.46966565 24.00858145 29.50409049 25.25151438 21.07277326 47.59241771 36.9923413 68.99011381 29.92722367 23.56615543 26.37780615 11.12187572 54.8404284 23.37836359 18.07054448 83.42254006 60.0783517 22.23636328 24.53856184 23.82632106 56.06520895 56.45060276 15.93637862 17.91386358 10.65335255 28.78971566 75.74960783 72.85014718 18.32964987 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type C14 CER 5282310 N-(OLEOYL)-CERAMIDE 5283563 N-PALMITOYLSPHINGOSINE 5283564 N-STEAROYLSPHINGOSINE 5283565 N-ICOSANOYLSPHINGOSINE 5283566 C24 CER 5283571 METABOLITES_END #END