#METABOLOMICS WORKBENCH araskind_20170802_131251 DATATRACK_ID:1175 STUDY_ID:ST000828 ANALYSIS_ID:AN001837 PROJECT_ID:PR000219 VERSION 1 CREATED_ON January 15, 2019, 12:36 pm #PROJECT PR:PROJECT_TITLE Metabolic regulation by PARP-1 in myofibroblast differentiation and fibrosis PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Analysis of wild type lung tissue after saline or bleomycin treatment PR:INSTITUTE University of Michigan PR:DEPARTMENT Deaprtment of Pathology PR:LABORATORY Sem H. Phan PR:LAST_NAME Hu PR:FIRST_NAME Biao PR:ADDRESS Ann Arbor, MI PR:EMAIL biaohu@med.umich.edu PR:PHONE 734-7635731 #STUDY ST:STUDY_TITLE Metabolic analysis of Parp1 ko/wt Saline & Bleo Mouse Lung Fiboblasts and Human ST:STUDY_TITLE IPF & Normal Lung Fiboblasts 3 ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Glycolysis/TCA/Nucleotide analysis (especially interested in ST:STUDY_SUMMARY alpha-ketoglutarate) and NAD+ and related metabolite analysis for all samples ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 6 ST:TOTAL_SUBJECTS 16 #SUBJECT SU:SUBJECT_TYPE HUMAN SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0004492 S00015469 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004493 S00015470 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004494 S00015471 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004495 S00015472 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004496 S00015473 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004497 S00015474 Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y SUBJECT_SAMPLE_FACTORS SU0004498 S00015475 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004499 S00015476 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004500 S00015477 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004501 S00015478 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004502 S00015479 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004503 S00015480 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004504 S00015481 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004505 S00015482 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004506 S00015483 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N SUBJECT_SAMPLE_FACTORS SU0004507 S00015484 Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Cultured cells #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A010-Ceramides.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent GC_7890N CH:COLUMN_NAME Agilent DB5-MS (30m × 0.25mm, 0.25um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A011-Glycolysis-TCA-nucleotides.pdf AN:ACQUISITION_PARAMETERS_FILE ALPHA KETO ACIDS-FULL.M #MS MS:INSTRUMENT_NAME Agilent 5975 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:MS_COMMENTS none #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples S00015469 S00015470 S00015471 S00015472 S00015473 S00015474 S00015475 S00015476 S00015477 S00015478 S00015479 S00015480 S00015481 S00015483 S00015484 Factors Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:N | saline/HLF:Y | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Tissues | Species:Mus musculus | bleomycin/IPF:Y | saline/HLF:N | wt B6:Y Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:N | saline/HLF:Y | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N Sample type:Cells | Species:Homo sapiens | bleomycin/IPF:Y | saline/HLF:N | wt B6:N PYRUVIC ACID 1.2120888 1.2122738 1.2166433 1.2240997 1.2343615 1.2364795 1.2239584 1.2458525 1.2510062 1.2534862 1.2427141 1.2304312 1.2203002 1.2220662 1.2503706 DL-ASPARTIC ACID 82.228826 83.121791 108.35697 208.59358 125.90848 193.98439 4.8687735 8.2218718 8.0346042 8.0066445 12.427014 6.3079602 4.6913311 3.7597118 10.857118 FUMARIC ACID 3.8291594 3.9963144 4.8130148 5.2062561 4.7341648 7.0042579 2.8785189 2.7818424 2.7896288 2.7916152 2.8256436 2.8390397 2.8076263 2.788486 3.1031532 2-KETOGLUTARIC ACID .2250877 .2279765 .2287517 .2308893 .2359683 .2380787 .2405373 .2504593 .2586114 .2526327 .2507883 .2645688 .2453656 .2402555 .2662557 L-ALANINE 2.1491892 2.4596988 4.2169022 7.72233 4.4421291 7.3234857 .5943472 1.0897502 1.3225341 1.1751392 1.6511848 2.0681367 1.3205668 1.1158445 4.0713104 L-GLUTAMINE 6.8331443 6.4461493 19.418498 31.933477 13.350189 19.513302 11.692336 20.719397 21.157957 22.825003 29.682731 14.900903 10.147254 8.7444129 22.633999 DL-GLUTAMIC ACID 6.3028675 6.1040444 8.6936756 14.290988 8.9094007 14.378001 2.8702344 4.8957194 4.775305 4.8792137 6.8351299 3.9097877 2.9760384 2.4428808 6.6109082 LACTIC ACID 1.9040661 3.0101775 2.9742998 6.3374418 .3731999 1.055963 1.1604786 .9431695 1.5286465 .3026353 .1891682 4.6088339 OXALACETIC ACID .1944054 .1944054 .1944054 .1944054 .1944054 .1944054 .1944054 .1944054 .1944054 .1944054 .1944054 .1944054 .1944054 .1944054 .1944054 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type PYRUVIC ACID 1060 DL-ASPARTIC ACID 424 FUMARIC ACID 444972 2-KETOGLUTARIC ACID 51 L-ALANINE 5950 L-GLUTAMINE 5961 DL-GLUTAMIC ACID 611 LACTIC ACID 612 OXALACETIC ACID 970 METABOLITES_END #END