#METABOLOMICS WORKBENCH araskind_20170802_131805 DATATRACK_ID:1177 STUDY_ID:ST000830 ANALYSIS_ID:AN001324 PROJECT_ID:PR000591 VERSION 1 CREATED_ON August 8, 2017, 12:26 pm #PROJECT PR:PROJECT_TITLE CD47 (integrin associated protein) metabolomics PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY CD47 WT/KO quad/serum (RNAseq mice) PR:INSTITUTE University of Michigan PR:DEPARTMENT Metabolism, Endo & Diabetes PR:LABORATORY Chun Lab PR:LAST_NAME Chun PR:FIRST_NAME Taehwa PR:ADDRESS Ann Arbor, MI PR:EMAIL taehwa@med.umich.edu PR:PHONE 734-763-3056 #STUDY ST:STUDY_TITLE CD47 WT/KO muscle and serum acyl-carnitine assay ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY These mice were fed a high-fat diet for 3 weeks, fast for 5 hours, dissected, ST:STUDY_SUMMARY and quadriceps were snap-frozen in LN2. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 7 ST:TOTAL_SUBJECTS 9 #SUBJECT SU:SUBJECT_TYPE MOUSE SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0013199 S00019644 Sample type:Quadriceps Muscle | Type:WT SUBJECT_SAMPLE_FACTORS SU0013200 S00019645 Sample type:Quadriceps Muscle | Type:WT SUBJECT_SAMPLE_FACTORS SU0013201 S00019646 Sample type:Quadriceps Muscle | Type:WT SUBJECT_SAMPLE_FACTORS SU0013202 S00019647 Sample type:Quadriceps Muscle | Type:WT SUBJECT_SAMPLE_FACTORS SU0013203 S00019648 Sample type:Quadriceps Muscle | Type:KO SUBJECT_SAMPLE_FACTORS SU0013204 S00019649 Sample type:Quadriceps Muscle | Type:KO SUBJECT_SAMPLE_FACTORS SU0013205 S00019650 Sample type:Quadriceps Muscle | Type:KO SUBJECT_SAMPLE_FACTORS SU0013206 S00019651 Sample type:Quadriceps Muscle | Type:KO SUBJECT_SAMPLE_FACTORS SU0013199 S00019652 Sample type:Serum | Type:WT SUBJECT_SAMPLE_FACTORS SU0013200 S00019653 Sample type:Serum | Type:WT SUBJECT_SAMPLE_FACTORS SU0013201 S00019654 Sample type:Serum | Type:WT SUBJECT_SAMPLE_FACTORS SU0013202 S00019655 Sample type:Serum | Type:WT SUBJECT_SAMPLE_FACTORS SU0013203 S00019656 Sample type:Serum | Type:KO SUBJECT_SAMPLE_FACTORS SU0013204 S00019657 Sample type:Serum | Type:KO SUBJECT_SAMPLE_FACTORS SU0013205 S00019658 Sample type:Serum | Type:KO SUBJECT_SAMPLE_FACTORS SU0013206 S00019659 Sample type:Serum | Type:KO SUBJECT_SAMPLE_FACTORS SU9999996 S00020332 Sample type:Pooled sample | Type:Pooled quadriceps muscle SUBJECT_SAMPLE_FACTORS SU9999996 S00020333 Sample type:Pooled sample | Type:Pooled serum #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A008_Acyl_Carnitines.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Waters Xbridge BEH C18 (50 x 1mm, 3.5um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A008 - Acyl Carnitines.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6490A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples S00019652 S00019653 S00019654 S00019655 S00019656 S00019657 S00019658 S00019659 Factors Sample type:Serum | Type:WT Sample type:Serum | Type:WT Sample type:Serum | Type:WT Sample type:Serum | Type:WT Sample type:Serum | Type:KO Sample type:Serum | Type:KO Sample type:Serum | Type:KO Sample type:Serum | Type:KO ACETYL-DL-CARNITINE 9.709912 14.652496 12.864938 16.32062 15.944876 10.939098 12.676002 12.978672 DECANOYLCARNITINE .0068476 .0080408 .0082688 .0137788 .03344 .007372 .0074404 .0099104 LAUROYLCARNITINE .0049856 .0077824 .0069236 .007714 .0079496 .0048488 .0052516 .007106 PROPIONYLCARNITINE .2071912 .2215096 .245632 .3771348 .2683332 .2951764 .2698532 .1990668 L-PALMITOYLCARNITINE .1249136 .1710304 .1876136 .1844976 .1468624 .1313736 .1262208 .1362376 O-OCTANOYLCARNITINE .0041876 .0060344 .0057076 .010526 .0068552 .0049552 .0048868 .0054872 BUTYRYLCARNITINE .4300536 .5108644 .3660464 .5180616 .4109244 .4533628 .3963628 .4341044 DL-CARNITINE 15.354888 10.135056 9.276408 12.981104 22.585376 17.884928 20.581712 12.517808 AC1MIY4G .0291156 .0480016 .0593636 .0632244 .0325356 .0369056 .029564 .0554192 ARACHIDONYL CARNITINE .003648 .0045752 .0034352 .0056088 .005396 .003952 .0044688 .0039672 LINOELAIDYL CARNITINE .0268128 .0363432 .0337744 .0474544 .0395656 .0325128 .024168 .0331056 18:3 CARNITINE .0008664 .0009728 .0007752 .000912 .0008664 .0006992 .0008208 .0007448 CIS-4-DECENOYL CARNITINE .0053732 .0075848 .007524 .0113088 .0215688 .0059584 .0071516 .0057456 ISOVALERYLCARNITINE .0208012 .031616 .0294424 .0444524 .0439964 .0280516 .060838 .0512772 MYRISTOYLCARNITINE .0277932 .0386688 .0388892 .0412604 .0338656 .0251028 .0248368 .0281884 STEAROYLCARNITINE .006612 .0082992 .0093632 .0060648 .028956 .0079344 .0044536 .0152456 OLEOYLCARNITINE .165376 .2728704 .2786768 .3159624 .237576 .222756 .175028 .2650728 GLUTARYLCARNITINE .0019152 .0036708 .0024852 .0049096 .002052 .0026752 .0027436 .0022724 (11Z,14Z)-ICOSADIENOYLCARNITINE .0047424 .0050312 .0043472 .0044384 .0044384 .0049552 .0029792 .0039824 3-HYDROXYLAUROYLCARNITINE .001064 .0012236 .0009652 .0010868 .0011932 .0008284 .0006232 .0007296 3-HYDROXYMYRISTOYLCARNITINE .0020748 .0026144 .0020444 .00304 .0018696 .0020824 .0012996 .0022192 3-HYDROXYPALMITOYLCARNITINE .002052 .0026296 .001824 .0024168 .0019608 .0021888 .0012008 .0021888 3-HYDROXYLINOLEYLCARNITINE .0028272 .004636 .0037544 .0050008 .0034504 .0039216 .002432 .0044232 16:1 CARNITINE .0142728 .0235144 .0202464 .020976 .0246696 .0167048 .0139384 .0230128 8:1 CARNITINE .0015276 .0017708 .0017404 .0017024 .0024396 .001102 .001482 .0007752 14:2 CARNITINE .0040584 .0065892 .008208 .0083524 .0096216 .0051756 .0046132 .0073872 12:1 CARNITINE .0023408 .004104 .004066 .0047576 .0059964 .0026752 .0023788 .0037544 14:1 CARNITINE .0176092 .03097 .0365484 .0384332 .0373464 .022838 .0229748 .0323836 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type ACETYL-DL-CARNITINE 1 DECANOYLCARNITINE 10245190 LAUROYLCARNITINE 10427569 PROPIONYLCARNITINE 107738 L-PALMITOYLCARNITINE 11953816 O-OCTANOYLCARNITINE 123701 BUTYRYLCARNITINE 213144 DL-CARNITINE 288 AC1MIY4G 3082079 ARACHIDONYL CARNITINE 53477832 LINOELAIDYL CARNITINE 53477834 18:3 CARNITINE 54500892 CIS-4-DECENOYL CARNITINE 57357170 ISOVALERYLCARNITINE 6426851 MYRISTOYLCARNITINE 6426854 STEAROYLCARNITINE 6426855 OLEOYLCARNITINE 6441392 GLUTARYLCARNITINE 71317118 (11Z,14Z)-ICOSADIENOYLCARNITINE 71464509 3-HYDROXYLAUROYLCARNITINE 71464535 3-HYDROXYMYRISTOYLCARNITINE 71464541 3-HYDROXYPALMITOYLCARNITINE 71464553 3-HYDROXYLINOLEYLCARNITINE 71464556 16:1 CARNITINE 76040365 8:1 CARNITINE 77908888 14:2 CARNITINE 87087940 12:1 CARNITINE 87087953 14:1 CARNITINE 87088002 METABOLITES_END #END