#METABOLOMICS WORKBENCH araskind_20170802_185416 DATATRACK_ID:1190 STUDY_ID:ST000839 ANALYSIS_ID:AN001345 PROJECT_ID:PR000542
VERSION 1
CREATED_ON August 9, 2017, 10:55 am
#PROJECT
PR:PROJECT_TITLE Thermal Modalities Study
PR:PROJECT_TYPE MS analysis
PR:PROJECT_SUMMARY This study is looking at the effect of cooling on muscle tissue metabolism
PR:INSTITUTE University of Michigan
PR:DEPARTMENT Orthopaedic Surgery
PR:LABORATORY Mendias Lab
PR:LAST_NAME Mendias
PR:FIRST_NAME Christopher
PR:ADDRESS Ann Arbor, MI
PR:EMAIL cmendias@umich.edu
PR:PHONE 734-764-3250
#STUDY
ST:STUDY_TITLE Effect of cooling on muscle tissue metabolism (part II)
ST:STUDY_TYPE MS analysis
ST:STUDY_SUMMARY Thermal modalities are commonly used in sports medicine to affect tissue
ST:STUDY_SUMMARY healing. Cold therapy is commonly used modalities, but the metabolic changes to
ST:STUDY_SUMMARY muscle after cooling are not known. The objective of this study is to look at
ST:STUDY_SUMMARY the effect of cooling on muscle metabolites and gene expression. There are a
ST:STUDY_SUMMARY total of 8 subjects in the study. Each subject had an ice cup cryotherapy
ST:STUDY_SUMMARY treatment ("cool" samples) to one leg for 15 minutes, and the other leg served
ST:STUDY_SUMMARY as the control ("cntrl" samples). Two hours after the application of
ST:STUDY_SUMMARY cryotherapy, a biopsy was taken from each thigh muscle. Muscle was minced with
ST:STUDY_SUMMARY scissors and quickly snap frozen in liquid nitrogen.
ST:INSTITUTE University of Michigan
ST:DEPARTMENT Biomedical Research Core Facilities
ST:LABORATORY Metabolomics core
ST:LAST_NAME Kachman
ST:FIRST_NAME Maureen
ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL mkachman@med.umich.edu
ST:PHONE (734) 232-8175
ST:NUM_GROUPS 10
ST:TOTAL_SUBJECTS 8
ST:STUDY_COMMENTS We would like to run the TCA Plus Analysis on each of the samples. For the
ST:STUDY_COMMENTS statistical analysis, we would normalize the cool side to the ctrl side, and
ST:STUDY_COMMENTS look at the relative change in metabolite abundance as a result of the cooling
ST:STUDY_COMMENTS procedure. only used in sports medicine to affect tissue healing.
#SUBJECT
SU:SUBJECT_TYPE HUMAN
SU:SUBJECT_SPECIES Homo sapiens
SU:TAXONOMY_ID 9606
#SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS SU0014908 S00023215 Temperature:Control Subject=1
SUBJECT_SAMPLE_FACTORS SU0014908 S00023216 Temperature:Cooled Subject=1
SUBJECT_SAMPLE_FACTORS SU0014909 S00023217 Temperature:Control Subject=2
SUBJECT_SAMPLE_FACTORS SU0014909 S00023218 Temperature:Cooled Subject=2
SUBJECT_SAMPLE_FACTORS SU0014910 S00023219 Temperature:Control Subject=3
SUBJECT_SAMPLE_FACTORS SU0014910 S00023220 Temperature:Cooled Subject=3
SUBJECT_SAMPLE_FACTORS SU0014911 S00023221 Temperature:Control Subject=4
SUBJECT_SAMPLE_FACTORS SU0014911 S00023222 Temperature:Cooled Subject=4
SUBJECT_SAMPLE_FACTORS SU0014912 S00023223 Temperature:Control Subject=5
SUBJECT_SAMPLE_FACTORS SU0014912 S00023224 Temperature:Cooled Subject=5
SUBJECT_SAMPLE_FACTORS SU0014913 S00023225 Temperature:Control Subject=6
SUBJECT_SAMPLE_FACTORS SU0014913 S00023226 Temperature:Cooled Subject=6
SUBJECT_SAMPLE_FACTORS SU0014914 S00023227 Temperature:Control Subject=7
SUBJECT_SAMPLE_FACTORS SU0014914 S00023228 Temperature:Cooled Subject=7
SUBJECT_SAMPLE_FACTORS SU0014915 S00023229 Temperature:Control Subject=8
SUBJECT_SAMPLE_FACTORS SU0014915 S00023230 Temperature:Cooled Subject=8
#COLLECTION
CO:COLLECTION_SUMMARY -
#TREATMENT
TR:TREATMENT_SUMMARY -
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY -
SP:SAMPLEPREP_PROTOCOL_FILENAME A011_Glycolysis-TCA-nucleotides.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE HILIC
CH:INSTRUMENT_NAME Agilent
CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um)
#ANALYSIS
AN:ANALYSIS_TYPE MS
AN:ANALYSIS_PROTOCOL_FILE A011 - Glycolysis-TCA-nucleotides.pdf
#MS
MS:MS_COMMENTS -
MS:INSTRUMENT_NAME Agilent 6520B QTOF
MS:INSTRUMENT_TYPE QTOF
MS:MS_TYPE ESI
MS:ION_MODE NEGATIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS nmol/mg
MS_METABOLITE_DATA_START
Samples S00023215 S00023216 S00023217 S00023218 S00023219 S00023220 S00023221 S00023222 S00023223 S00023224 S00023225 S00023226 S00023227 S00023228 S00023229 S00023230
Factors Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled
D-ERYTHROSE 4-PHOSPHATE 3.94646415 5.227754209 3.417357853 1.933654392 3.312279724 4.009106573 3.87202955 5.005469238 3.25329155 2.858650942 3.227766298 2.545112891 4.389657105 2.300372952 3.694400552 3.074067477
BETA-NAD+ .061383361 .094627856 .027148456 .016684607 .045028167 .011073473 .036870057 .027876861 .063795149 .035547528 .046566157 .108090497 .014606847 .077303078 .095719458 .108653926
COENZYME II .008207605 .006789212 .003144356 .002838797 .004040534 .002803397 .004234083 .00529144 .004647351 .003184894 .006466174 .005539331 .002119936 .004692058 .009093466 .010877024
SEDOHEPTULOSE 7-PHOSPHATE .00386658 .011162527 .002623441 .006653065 .002408949 .005335913 .003042897 .005404464 .003185302 .002275792 .007054431 .005453507 .008057386 .0035053 .003647809 .003262038
D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 1.269638109 .947416422 3.337867273 2.581308631 2.3273682 5.461056018 2.510364683 3.340046292 1.443928121 1.403804465 2.041791959 .781649437 1.843231206 .798620372 1.499817772 2.122147139
CITRIC ACID .081620299 .11481453 .066870211 .101163017 .152750345 .069848955 .08596618 .078378802 .162370123 .104068247 .182294429 .212417167 .063425171 .090186501 .260857311 .265800033
NADH .034282857 .045283412 .008360933 .030189146 .004401122 .07944624 .143262691 .078200061 .039511913 .176692706 .037573807 .07442283
FAD .010692553 .009597777 .007229298 .006092064 .00745574 .008345892 .007812437 .008195953 .007575984 .005975604 .008802507 .007117713 .006868486 .005984764 .013238667 .012357041
MALIC ACID 2.263102004 2.352403016 .894772328 .684167038 .761412689 .583195476 .914080484 .912978189 1.509675156 .758849478 2.525787507 3.076414962 1.037029319 .928601608 3.834833377 2.94478518
ADENOSINE TRIPHOSPHATE 2.519619294 2.556889038 .577001218 1.089223982 1.759796408 .392326337 2.026415602 1.411050083 2.316626289 1.5867462 2.261485404 2.264618889 .069146763 1.926661601 3.712126911 4.320597983
ROBISON ESTER 5.179479675 7.504835951 5.912119801 2.895636804 4.554954998 6.146420964 5.195052378 7.166376134 4.079391887 4.057755034 3.862134473 2.506050855 6.889751917 3.182842958 5.05705298 3.855391538
SUCROSE .169419199 .216134305 .295964022 .064927787 .047655018 .095066608 .086963055 .148015296 .080620477 .055596389 .105486143 .11323052 .389051843 .075397519 .176821841 .172603545
ADENOSINE 5'-DIPHOSPHATE .233946275 .239159323 .235614242 .210016908 .166813823 .325091614 .240027999 .227609424 .097709442 .095601901 .159951895 .109053017 .247691503 .100201306 .157065873 .178207686
ADENOSINE 5'-MONOPHOSPHATE .006770042 .009717114 .022453876 .010257355 .007842336 .015638742 .012829213 .015617282 .00833924 .007443757 .00612996 .005996644 .040161748 .008225998 .011075174 .013638223
GLYCERATE 3-PHOSPHATE 1.849566003 1.424811546 .512046675 .606554663 .724307902 .341281053 .525315952 .866275454 .58941218 .731903207 1.592912412 1.290033333 .249406339 .749362426 1.874208785 2.175277715
RIBOSE PHOSPHATE .172536194 .152353992 .200054901 .150771251 .097546661 .200870874 .116652625 .203337117 .073111602 .095920726 .162116805 .100856198 .406365077 .068877287 .131898038 .107317074
6-PHOSPHOGLUCONIC ACID .019584558 .016002341 .008921812 .010236046 .010326799 .009985939 .010706581 .01540841 .011336271 .01285533 .021928284 .015842749 .00735747 .010816324 .016563475 .015062229
ACETOACETYL-COA .008904697 .008970614 .009052976 .00901687 .00903461 .009480267 .009234711 .00938678 .009104327 .00909944 .009018288 .008885126 .009439027 .00907401 .008924986
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type
D-ERYTHROSE 4-PHOSPHATE 122357
BETA-NAD+ 15938971
COENZYME II 15938972
SEDOHEPTULOSE 7-PHOSPHATE 165007
D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 21125049
CITRIC ACID 311
NADH 439153
FAD 46906035
MALIC ACID 525
ADENOSINE TRIPHOSPHATE 5957
ROBISON ESTER 5958
SUCROSE 5988
ADENOSINE 5'-DIPHOSPHATE 6022
ADENOSINE 5'-MONOPHOSPHATE 6083
GLYCERATE 3-PHOSPHATE 724
RIBOSE PHOSPHATE 77982
6-PHOSPHOGLUCONIC ACID 91493
ACETOACETYL-COA 92153
METABOLITES_END
#END