#METABOLOMICS WORKBENCH araskind_20170802_132352 DATATRACK_ID:1180 STUDY_ID:ST000841 ANALYSIS_ID:AN001354 PROJECT_ID:PR000599 VERSION 1 CREATED_ON August 9, 2017, 11:32 am #PROJECT PR:PROJECT_TITLE Prelim data for NIH exercise consortium PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Plasma samples before and after exercise training in obese adults PR:INSTITUTE University of Michigan PR:DEPARTMENT Kinesiology PR:LABORATORY Horowitz Lab PR:LAST_NAME Horowitz PR:FIRST_NAME Jeff PR:ADDRESS Ann Arbor, MI PR:EMAIL jeffhoro@umich.edu PR:PHONE 734-647-3354 #STUDY ST:STUDY_TITLE Metabolomics of muscle in insulin sensitive and resistant obese indivduals. ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY "OC" samples: muscle biopsy samples collected from insulin sensitive / insulin ST:STUDY_SUMMARY resistant obese indivduals before undergoing a hyperinsulinemic-euglycemic ST:STUDY_SUMMARY clamp. Subjects were previously categorized for the rate of fatty acid release ST:STUDY_SUMMARY from adipose tissue into the bloodstream as a measure of insuline resistance. ST:STUDY_SUMMARY Low-FA, Med-FA, and High-FA stand for low, medium, and high rate of release. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 28 #SUBJECT SU:SUBJECT_TYPE HUMAN SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0013583 S00020427 Subject group:LOW-FA SUBJECT_SAMPLE_FACTORS SU0013584 S00020428 Subject group:LOW-FA SUBJECT_SAMPLE_FACTORS SU0013585 S00020429 Subject group:MID-FA SUBJECT_SAMPLE_FACTORS SU0013586 S00020430 Subject group:LOW-FA SUBJECT_SAMPLE_FACTORS SU0013587 S00020431 Subject group:LOW-FA SUBJECT_SAMPLE_FACTORS SU0013588 S00020432 Subject group:MID-FA SUBJECT_SAMPLE_FACTORS SU0013589 S00020433 Subject group:LOW-FA SUBJECT_SAMPLE_FACTORS SU0013590 S00020434 Subject group:LOW-FA SUBJECT_SAMPLE_FACTORS SU0013591 S00020435 Subject group:MID-FA SUBJECT_SAMPLE_FACTORS SU0013592 S00020436 Subject group:LOW-FA SUBJECT_SAMPLE_FACTORS SU0013593 S00020437 Subject group:MID-FA SUBJECT_SAMPLE_FACTORS SU0013594 S00020438 Subject group:MID-FA SUBJECT_SAMPLE_FACTORS SU0013595 S00020439 Subject group:HIGH-FA SUBJECT_SAMPLE_FACTORS SU0013596 S00020440 Subject group:HIGH-FA SUBJECT_SAMPLE_FACTORS SU0013597 S00020441 Subject group:MID-FA SUBJECT_SAMPLE_FACTORS SU0013598 S00020442 Subject group:LOW-FA SUBJECT_SAMPLE_FACTORS SU0013599 S00020443 Subject group:MID-FA SUBJECT_SAMPLE_FACTORS SU0013600 S00020444 Subject group:MID-FA SUBJECT_SAMPLE_FACTORS SU0013601 S00020445 Subject group:HIGH-FA SUBJECT_SAMPLE_FACTORS SU0013602 S00020446 Subject group:MID-FA SUBJECT_SAMPLE_FACTORS SU0013603 S00020447 Subject group:HIGH-FA SUBJECT_SAMPLE_FACTORS SU0013604 S00020448 Subject group:HIGH-FA SUBJECT_SAMPLE_FACTORS SU0013605 S00020449 Subject group:HIGH-FA SUBJECT_SAMPLE_FACTORS SU0013606 S00020450 Subject group:HIGH-FA SUBJECT_SAMPLE_FACTORS SU0013607 S00020451 Subject group:HIGH-FA SUBJECT_SAMPLE_FACTORS SU0013608 S00020452 Subject group:HIGH-FA SUBJECT_SAMPLE_FACTORS SU0013609 S00020453 Subject group:N/A SUBJECT_SAMPLE_FACTORS SU0013610 S00020454 Subject group:N/A #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A003_-_Untargeted_Metabolomics.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A011 - Glycolysis-TCA-nucleotides.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6520B QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS counts MS_METABOLITE_DATA_START Samples S00020427 S00020428 S00020429 S00020430 S00020431 S00020432 S00020433 S00020434 S00020435 S00020436 S00020437 S00020438 S00020439 S00020440 S00020441 S00020442 S00020443 S00020444 S00020445 S00020446 S00020447 S00020448 S00020449 S00020450 S00020451 S00020452 S00020453 S00020454 Factors Subject group:LOW-FA Subject group:LOW-FA Subject group:MID-FA Subject group:LOW-FA Subject group:LOW-FA Subject group:MID-FA Subject group:LOW-FA Subject group:LOW-FA Subject group:MID-FA Subject group:LOW-FA Subject group:MID-FA Subject group:MID-FA Subject group:HIGH-FA Subject group:HIGH-FA Subject group:MID-FA Subject group:LOW-FA Subject group:MID-FA Subject group:MID-FA Subject group:HIGH-FA Subject group:MID-FA Subject group:HIGH-FA Subject group:HIGH-FA Subject group:HIGH-FA Subject group:HIGH-FA Subject group:HIGH-FA Subject group:HIGH-FA Subject group:N/A Subject group:N/A XANTHINE 26689.93178 45902.67828 20041.25733 18048.26891 9528.008163 14656.39163 16739.79849 35493.66786 13720.2477 39134.38796 26672.86875 24035.53044 17063.84973 52336.53395 22143.24879 20319.24314 32530.88708 38152.37554 9082.343265 21467.4703 7871.269115 17277.46739 20150.40389 26625.23535 28579.21371 21810.80025 6099.77381 9038.278755 HEXOPYRANOSE 634796.0177 388837.7503 751207.6071 1165826.004 222134.2184 1125098.81 379604.3293 538982.25 341874.6256 921605.7801 889971.1277 558235.2102 628497.69 766501.517 475313.8474 736491.2559 551843.1799 674684.9437 570465.769 409482.6263 108996.3677 959570.9322 949030.1155 714571.4872 476309.0034 569285.8932 560183.0049 161764.9821 DL-ASPARTIC ACID 27616.16372 10278.11806 254442.5678 59936.21008 21797.71293 185112.0406 32782.77875 88711.75833 9599.458525 46397.71569 30394.12054 23776.7541 26091.61792 281447.0714 24260.68999 9420.106433 28842.0575 16827.08442 219376.0404 95288.78534 55651.41247 200019.0311 21869.9417 103390.3416 50238.12686 148501.3552 210927.6793 110907.0498 OLEIC ACID 1203049.168 1214699.419 1241313.714 2076685.819 766614.8952 3242487.75 2211928.279 598105.2333 286561.7535 1369121.228 1031814.254 641323.5029 1276637.174 11505487.79 2724982.176 2186612.296 1592522.506 1329979.233 627953.2 759824.012 584158.674 662515.5911 997401.5741 1236840.447 920366.3006 1297166.25 897896.7927 370682.7597 STEARIC ACID 3778217.021 1569072.231 3015039.192 10641710.55 1548595.318 4295026.334 1348759.664 1849461.163 1360895.81 3482676.231 2753021.827 2082617.661 2122041.776 2965984.673 1793259.525 1442781.643 1143673.991 2662839.16 976195.8849 926146.2891 664925.8496 905653.3038 1434613.838 1965614.258 2375691.105 1502144.106 771459.1772 407333.5707 L-GLUTAMINE 30227189.23 19700803.13 30952393 44713416.93 13903567.74 23705197.42 9945924.322 29805087.01 15453187.59 27074157.29 25197674.51 13921234.89 20139078.88 16916492.88 19638886.16 15846157.68 11233820.32 28562776.39 15431918.15 15952873.94 3818826.208 17540199.96 15181320.48 21545297.55 15984958.89 11664423.84 22790042.31 4830095.622 DL-GLUTAMIC ACID 6216740.043 1579417.994 8799395.182 9437924.034 4674635.018 4391661.75 2352453.607 7021379.475 2886743.887 7394113.485 5941937.29 3664898.923 5184361.35 3376435.217 5126959.913 3686482.184 3915586.586 7504440.281 4193323.832 3521015.3 2278209.936 5431827.818 5294941.842 5668048.039 6755700.689 6296754.647 5277665.073 2167154.742 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type XANTHINE 1188 HEXOPYRANOSE 206 DL-ASPARTIC ACID 424 OLEIC ACID 445639 STEARIC ACID 5281 L-GLUTAMINE 5961 DL-GLUTAMIC ACID 611 METABOLITES_END #END