#METABOLOMICS WORKBENCH hormel101_20170811_093927_mwtab.txt DATATRACK_ID:1216 STUDY_ID:ST000866 ANALYSIS_ID:AN001419 PROJECT_ID:PR000575 VERSION 1 CREATED_ON October 2, 2017, 4:45 pm #PROJECT PR:PROJECT_TITLE Mayo Pilot and Feasiblity: Targeting Myelin Metabolism of Kallikrein 6 Signals PR:PROJECT_TITLE through PAR1 and PAR2 to Enchance Recovery of Function after SCI PR:PROJECT_SUMMARY CNS trauma generates a proteolytic imbalance contributing to secondary injury, PR:PROJECT_SUMMARY including axonopathy and neuron degeneration. Kallikrein 6 (Klk6) is a serine PR:PROJECT_SUMMARY protease implicated in neurodegeneration and here we investigate the role of PR:PROJECT_SUMMARY protease activated receptors 1 (PAR1) and PAR2 in mediating these effects in PR:PROJECT_SUMMARY mice with spinal cord injury (SCI). This project conducts untargeted Profiling PR:PROJECT_SUMMARY (unbiased metabolomics assay) and targeted lipid analysis. The lipid assays are PR:PROJECT_SUMMARY 1) free fatty acid composition of lipids; 2) free fatty acid panel; 3) PR:PROJECT_SUMMARY cholesterol concentration (free and bound); 4) Ceramides, including galactosyl PR:PROJECT_SUMMARY and glucosyl; 5) sphingomyelin. PR:INSTITUTE Mayo Clinic PR:LAST_NAME Scarisbrick PR:FIRST_NAME Isobel PR:ADDRESS 200 First Street SW, Rochester, MN 55905 PR:EMAIL scarisbrick.isobel@mayo.edu PR:PHONE 507-284-0124 #STUDY ST:STUDY_TITLE Large Untargeted Profiling in PAR1 and PAR2 Mice after SCI ST:STUDY_SUMMARY Large Untargeted Profiling in NEFA in PAR1 and PAR2 Mice after SCI. The samples ST:STUDY_SUMMARY submitted are purified myelin preparations from the postnatal day 21 or 60 mouse ST:STUDY_SUMMARY spinal cord (SC). There are 30 total sampes, n=5 for each group. wild type ST:STUDY_SUMMARY control, PAR1 KO and PAR2 KO at P21 and P60 days. ST:INSTITUTE Mayo Clinic ST:LAST_NAME Scarisbrick ST:FIRST_NAME Isobel ST:ADDRESS 200 First Street SW, Rochester, MN 55905 ST:EMAIL scarisbrick.isobel@mayo.edu ST:PHONE 507-284-0124 #SUBJECT SU:SUBJECT_TYPE Mouse SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS ms6380-1 nC18-24oct16-001-r001 Group:PAR2-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-1 nC18-24oct16-001-r002 Group:PAR2-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-2 nC18-24oct16-002-r001 Group:PAR2-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-2 nC18-24oct16-002-r002 Group:PAR2-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-3 nC18-24oct16-003-r001 Group:WT-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-3 nC18-24oct16-003-r002 Group:WT-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-4 nC18-24oct16-004-r001 Group:PAR2-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-4 nC18-24oct16-004-r002 Group:PAR2-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-5 nC18-24oct16-005-r001 Group:WT-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-5 nC18-24oct16-005-r002 Group:WT-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-6 nC18-24oct16-006-r001 Group:PAR2-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-6 nC18-24oct16-006-r002 Group:PAR2-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-7 nC18-24oct16-007-r001 Group:PAR2-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-7 nC18-24oct16-007-r002 Group:PAR2-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-8 nC18-24oct16-008-r001 Group:PAR1-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-8 nC18-24oct16-008-r002 Group:PAR1-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-9 nC18-24oct16-009-r001 Group:WT-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-9 nC18-24oct16-009-r002 Group:WT-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-10 nC18-24oct16-010-r001 Group:PAR2-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-10 nC18-24oct16-010-r002 Group:PAR2-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-11 nC18-24oct16-011-r001 Group:WT-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-11 nC18-24oct16-011-r002 Group:WT-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-12 nC18-24oct16-012-r001 Group:PAR1-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-12 nC18-24oct16-012-r002 Group:PAR1-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-13 nC18-24oct16-013-r001 Group:WT-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-13 nC18-24oct16-013-r002 Group:WT-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-14 nC18-24oct16-014-r001 Group:PAR1-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-14 nC18-24oct16-014-r002 Group:PAR1-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-15 nC18-24oct16-015-r001 Group:WT-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-15 nC18-24oct16-015-r002 Group:WT-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-16 nC18-24oct16-016-r001 Group:PAR2-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-16 nC18-24oct16-016-r002 Group:PAR2-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-17 nC18-24oct16-017-r001 Group:WT-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-17 nC18-24oct16-017-r002 Group:WT-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-18 nC18-24oct16-018-r001 Group:PAR1-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-18 nC18-24oct16-018-r002 Group:PAR1-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-19 nC18-24oct16-019-r001 Group:WT-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-19 nC18-24oct16-019-r002 Group:WT-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-20 nC18-24oct16-020-r001 Group:PAR1-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-20 nC18-24oct16-020-r002 Group:PAR1-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-21 nC18-24oct16-021-r001 Group:PAR1-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-21 nC18-24oct16-021-r002 Group:PAR1-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-22 nC18-24oct16-022-r001 Group:PAR1-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-22 nC18-24oct16-022-r002 Group:PAR1-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-23 nC18-24oct16-023-r001 Group:WT-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-23 nC18-24oct16-023-r002 Group:WT-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-24 nC18-24oct16-024-r001 Group:PAR1-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-24 nC18-24oct16-024-r002 Group:PAR1-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-25 nC18-24oct16-025-r001 Group:PAR1-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-25 nC18-24oct16-025-r002 Group:PAR1-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-26 nC18-24oct16-026-r001 Group:PAR2-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-26 nC18-24oct16-026-r002 Group:PAR2-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-27 nC18-24oct16-027-r001 Group:PAR1-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-27 nC18-24oct16-027-r002 Group:PAR1-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-28 nC18-24oct16-028-r001 Group:WT-Control rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-28 nC18-24oct16-028-r002 Group:WT-Control rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-29 nC18-24oct16-029-r001 Group:PAR2-30d rep=1; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-29 nC18-24oct16-029-r002 Group:PAR2-30d rep=2; ms=NC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-1 pC18-19oct16-001-r001 Group:PAR2-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-1 pC18-19oct16-001-r002 Group:PAR2-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-2 pC18-19oct16-002-r001 Group:PAR2-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-2 pC18-19oct16-002-r002 Group:PAR2-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-3 pC18-19oct16-003-r001 Group:WT-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-3 pC18-19oct16-003-r002 Group:WT-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-4 pC18-19oct16-004-r001 Group:PAR2-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-4 pC18-19oct16-004-r002 Group:PAR2-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-5 pC18-19oct16-005-r001 Group:WT-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-5 pC18-19oct16-005-r002 Group:WT-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-6 pC18-19oct16-006-r001 Group:PAR2-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-6 pC18-19oct16-006-r002 Group:PAR2-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-7 pC18-19oct16-007-r001 Group:PAR2-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-7 pC18-19oct16-007-r002 Group:PAR2-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-8 pC18-19oct16-008-r001 Group:PAR1-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-8 pC18-19oct16-008-r002 Group:PAR1-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-9 pC18-19oct16-009-r001 Group:WT-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-9 pC18-19oct16-009-r002 Group:WT-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-10 pC18-19oct16-010-r001 Group:PAR2-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-10 pC18-19oct16-010-r002 Group:PAR2-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-11 pC18-19oct16-011-r001 Group:WT-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-11 pC18-19oct16-011-r002 Group:WT-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-12 pC18-19oct16-012-r001 Group:PAR1-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-12 pC18-19oct16-012-r002 Group:PAR1-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-13 pC18-19oct16-013-r001 Group:WT-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-13 pC18-19oct16-013-r002 Group:WT-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-14 pC18-19oct16-014-r001 Group:PAR1-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-14 pC18-19oct16-014-r002 Group:PAR1-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-15 pC18-19oct16-015-r001 Group:WT-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-15 pC18-19oct16-015-r002 Group:WT-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-16 pC18-19oct16-016-r001 Group:PAR2-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-16 pC18-19oct16-016-r002 Group:PAR2-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-17 pC18-19oct16-017-r001 Group:WT-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-17 pC18-19oct16-017-r002 Group:WT-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-18 pC18-19oct16-018-r001 Group:PAR1-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-18 pC18-19oct16-018-r002 Group:PAR1-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-19 pC18-19oct16-019-r001 Group:WT-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-19 pC18-19oct16-019-r002 Group:WT-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-20 pC18-19oct16-020-r001 Group:PAR1-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-20 pC18-19oct16-020-r002 Group:PAR1-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-21 pC18-19oct16-021-r001 Group:PAR1-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-21 pC18-19oct16-021-r002 Group:PAR1-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-22 pC18-19oct16-022-r001 Group:PAR1-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-22 pC18-19oct16-022-r002 Group:PAR1-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-23 pC18-19oct16-023-r001 Group:WT-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-23 pC18-19oct16-023-r002 Group:WT-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-24 pC18-19oct16-024-r001 Group:PAR1-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-24 pC18-19oct16-024-r002 Group:PAR1-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-25 pC18-19oct16-025-r001 Group:PAR1-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-25 pC18-19oct16-025-r002 Group:PAR1-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-26 pC18-19oct16-026-r001 Group:PAR2-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-26 pC18-19oct16-026-r002 Group:PAR2-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-27 pC18-19oct16-027-r001 Group:PAR1-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-27 pC18-19oct16-027-r002 Group:PAR1-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-28 pC18-19oct16-028-r001 Group:WT-Control rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-28 pC18-19oct16-028-r002 Group:WT-Control rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-29 pC18-19oct16-029-r001 Group:PAR2-30d rep=1; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-29 pC18-19oct16-029-r002 Group:PAR2-30d rep=2; ms=PC18; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-1 nhilic-31oct16-001-r001 Group:PAR2-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-1 nhilic-31oct16-001-r002 Group:PAR2-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-2 nhilic-31oct16-002-r001 Group:PAR2-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-2 nhilic-31oct16-002-r002 Group:PAR2-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-3 nhilic-31oct16-003-r001 Group:WT-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-3 nhilic-31oct16-003-r002 Group:WT-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-4 nhilic-31oct16-004-r001 Group:PAR2-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-4 nhilic-31oct16-004-r002 Group:PAR2-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-5 nhilic-31oct16-005-r001 Group:WT-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-5 nhilic-31oct16-005-r002 Group:WT-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-6 nhilic-31oct16-006-r001 Group:PAR2-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-6 nhilic-31oct16-006-r002 Group:PAR2-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-7 nhilic-31oct16-007-r001 Group:PAR2-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-7 nhilic-31oct16-007-r002 Group:PAR2-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-8 nhilic-31oct16-008-r001 Group:PAR1-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-8 nhilic-31oct16-008-r002 Group:PAR1-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-9 nhilic-31oct16-009-r001 Group:WT-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-9 nhilic-31oct16-009-r002 Group:WT-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-10 nhilic-31oct16-010-r001 Group:PAR2-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-10 nhilic-31oct16-010-r002 Group:PAR2-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-11 nhilic-31oct16-011-r001 Group:WT-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-11 nhilic-31oct16-011-r002 Group:WT-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-12 nhilic-31oct16-012-r001 Group:PAR1-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-12 nhilic-31oct16-012-r002 Group:PAR1-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-13 nhilic-31oct16-013-r001 Group:WT-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-13 nhilic-31oct16-013-r002 Group:WT-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-14 nhilic-31oct16-014-r001 Group:PAR1-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-14 nhilic-31oct16-014-r002 Group:PAR1-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-15 nhilic-31oct16-015-r001 Group:WT-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-15 nhilic-31oct16-015-r002 Group:WT-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-16 nhilic-31oct16-016-r001 Group:PAR2-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-16 nhilic-31oct16-016-r002 Group:PAR2-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-17 nhilic-31oct16-017-r001 Group:WT-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-17 nhilic-31oct16-017-r002 Group:WT-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-18 nhilic-31oct16-018-r001 Group:PAR1-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-18 nhilic-31oct16-018-r002 Group:PAR1-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-19 nhilic-31oct16-019-r001 Group:WT-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-19 nhilic-31oct16-019-r002 Group:WT-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-20 nhilic-31oct16-020-r001 Group:PAR1-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-20 nhilic-31oct16-020-r002 Group:PAR1-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-21 nhilic-31oct16-021-r001 Group:PAR1-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-21 nhilic-31oct16-021-r002 Group:PAR1-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-22 nhilic-31oct16-022-r001 Group:PAR1-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-22 nhilic-31oct16-022-r002 Group:PAR1-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-23 nhilic-31oct16-023-r001 Group:WT-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-23 nhilic-31oct16-023-r002 Group:WT-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-24 nhilic-31oct16-024-r001 Group:PAR1-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-24 nhilic-31oct16-024-r002 Group:PAR1-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-25 nhilic-31oct16-025-r001 Group:PAR1-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-25 nhilic-31oct16-025-r002 Group:PAR1-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-26 nhilic-31oct16-026-r001 Group:PAR2-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-26 nhilic-31oct16-026-r002 Group:PAR2-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-27 nhilic-31oct16-027-r001 Group:PAR1-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-27 nhilic-31oct16-027-r002 Group:PAR1-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-28 nhilic-31oct16-028-r001 Group:WT-Control rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-28 nhilic-31oct16-028-r002 Group:WT-Control rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-29 nhilic-31oct16-029-r001 Group:PAR2-30d rep=1; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-29 nhilic-31oct16-029-r002 Group:PAR2-30d rep=2; ms=NHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-1 philic-28oct16-001-r001 Group:PAR2-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-1 philic-28oct16-001-r002 Group:PAR2-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-2 philic-28oct16-002-r001 Group:PAR2-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-2 philic-28oct16-002-r002 Group:PAR2-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-3 philic-28oct16-003-r001 Group:WT-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-3 philic-28oct16-003-r002 Group:WT-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-4 philic-28oct16-004-r001 Group:PAR2-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-4 philic-28oct16-004-r002 Group:PAR2-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-5 philic-28oct16-005-r001 Group:WT-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-5 philic-28oct16-005-r002 Group:WT-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-6 philic-28oct16-006-r001 Group:PAR2-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-6 philic-28oct16-006-r002 Group:PAR2-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-7 philic-28oct16-007-r001 Group:PAR2-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-7 philic-28oct16-007-r002 Group:PAR2-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-8 philic-28oct16-008-r001 Group:PAR1-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-8 philic-28oct16-008-r002 Group:PAR1-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-9 philic-28oct16-009-r001 Group:WT-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-9 philic-28oct16-009-r002 Group:WT-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-10 philic-28oct16-010-r001 Group:PAR2-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-10 philic-28oct16-010-r002 Group:PAR2-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-11 philic-28oct16-011-r001 Group:WT-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-11 philic-28oct16-011-r002 Group:WT-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-12 philic-28oct16-012-r001 Group:PAR1-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-12 philic-28oct16-012-r002 Group:PAR1-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-13 philic-28oct16-013-r001 Group:WT-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-13 philic-28oct16-013-r002 Group:WT-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-14 philic-28oct16-014-r001 Group:PAR1-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-14 philic-28oct16-014-r002 Group:PAR1-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-15 philic-28oct16-015-r001 Group:WT-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-15 philic-28oct16-015-r002 Group:WT-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-16 philic-28oct16-016-r001 Group:PAR2-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-16 philic-28oct16-016-r002 Group:PAR2-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-17 philic-28oct16-017-r001 Group:WT-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-17 philic-28oct16-017-r002 Group:WT-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-18 philic-28oct16-018-r001 Group:PAR1-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-18 philic-28oct16-018-r002 Group:PAR1-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-19 philic-28oct16-019-r001 Group:WT-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-19 philic-28oct16-019-r002 Group:WT-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-20 philic-28oct16-020-r001 Group:PAR1-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-20 philic-28oct16-020-r002 Group:PAR1-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-21 philic-28oct16-021-r001 Group:PAR1-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-21 philic-28oct16-021-r002 Group:PAR1-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-22 philic-28oct16-022-r001 Group:PAR1-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-22 philic-28oct16-022-r002 Group:PAR1-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-23 philic-28oct16-023-r001 Group:WT-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-23 philic-28oct16-023-r002 Group:WT-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-24 philic-28oct16-024-r001 Group:PAR1-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-24 philic-28oct16-024-r002 Group:PAR1-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-25 philic-28oct16-025-r001 Group:PAR1-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-25 philic-28oct16-025-r002 Group:PAR1-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-26 philic-28oct16-026-r001 Group:PAR2-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-26 philic-28oct16-026-r002 Group:PAR2-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-27 philic-28oct16-027-r001 Group:PAR1-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-27 philic-28oct16-027-r002 Group:PAR1-30d rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-28 philic-28oct16-028-r001 Group:WT-Control rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-28 philic-28oct16-028-r002 Group:WT-Control rep=2; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-29 philic-28oct16-029-r001 Group:PAR2-30d rep=1; ms=PHILIC; Tissue=Spinal Cord SUBJECT_SAMPLE_FACTORS ms6380-29 philic-28oct16-029-r002 Group:PAR2-30d rep=2; ms=PHILIC; Tissue=Spinal Cord #COLLECTION CO:COLLECTION_SUMMARY This prior project involved frozen whole mouse spinal cord (SC) samples for CO:COLLECTION_SUMMARY Untargeted Profiling (unbiased metabolomics assay). There were 9 samples total, CO:COLLECTION_SUMMARY n=3 for each genotype that includes wild type control, PAR1 KO and PAR2 KO at CO:COLLECTION_SUMMARY P60 days. Your team prepared the samples and ran the profiling and provided us CO:COLLECTION_SUMMARY with the results. Since the outcome of this preliminary study using 3 animals CO:COLLECTION_SUMMARY for each genotype looked so promising, we discussed bringing the total number to CO:COLLECTION_SUMMARY 5 and re-running to improve the power. Therefore, we are submitting 2 more CO:COLLECTION_SUMMARY samples for each genotype at P60. In addition, we are providing 5 samples for CO:COLLECTION_SUMMARY each genotype at P21 (an earlier age) for additional comparisons. To bring n=5 CO:COLLECTION_SUMMARY for each P60 genotype we provide 2 more samples per genotype. In addition we CO:COLLECTION_SUMMARY provide n=5 for P21 for each genotype (wild type, PAR1 and PAR2). #TREATMENT TR:TREATMENT_SUMMARY A 3g Clip produces moderate SCI including demyelination and clinical impairment TR:TREATMENT_SUMMARY and we recently published a detailed methodology. At 1 week after injury, the 3g TR:TREATMENT_SUMMARY injured mice are expected to have an average Basso Mouse Scale score (BMS)=5 on TR:TREATMENT_SUMMARY a 9 point scale such that they have frequent plantar stepping with no or some TR:TREATMENT_SUMMARY coordination. This level of impairment was chosen to provide a sufficient window TR:TREATMENT_SUMMARY to observe recovery. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY "large scale profiling of mouse spinal cord Lipids will be quantified in myelin SP:SAMPLEPREP_SUMMARY isolated in high yield and purity by subcellular fractionation from the SP:SAMPLEPREP_SUMMARY lumbosacral spinal cord. While there are no absolutely ‘myelin-specific’ SP:SAMPLEPREP_SUMMARY lipids, galactocerebroside is the most typical of myelin in the adult nervous SP:SAMPLEPREP_SUMMARY system being directly proportional to the amount of myelin. Sulfatide is another SP:SAMPLEPREP_SUMMARY galactolipid enriched in myelin. Together with cholesterol, these form 78% of SP:SAMPLEPREP_SUMMARY the total amount of lipid in the myelin membrane and each will be quantified SP:SAMPLEPREP_SUMMARY using LC/MS/MS. A highly sensitive assay for galactocerebroside was recently SP:SAMPLEPREP_SUMMARY established by the Mayo Metabolomics Core and can be implemented immediately. SP:SAMPLEPREP_SUMMARY The LC/MS/MS panel for free fatty acids, including the very long chain fatty SP:SAMPLEPREP_SUMMARY acids found in myelin is also routinely performed by the Core. Cholesterol will SP:SAMPLEPREP_SUMMARY be quantified using an NMR-based approach by the Mayo Dept. of Laboratory SP:SAMPLEPREP_SUMMARY Medicine Clinical Core. Additionally, we have a plan in place with the SP:SAMPLEPREP_SUMMARY Metabolomics Core to develop LC/MS/MS assays for sulfatide and sphingomyelin SP:SAMPLEPREP_SUMMARY during the Pilot proposal. Having quantitative assays for each of these key SP:SAMPLEPREP_SUMMARY myelin lipids will facilitate our goal to comprehensively profile myelin lipid SP:SAMPLEPREP_SUMMARY metabolism and will form foundational assays for a future NIH grant focused on SP:SAMPLEPREP_SUMMARY myelin metabolism." #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1290 Infinity CH:COLUMN_NAME Waters Acquity HSS C18 (150 x 2.1mm, 1.8um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Agilent 6550 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_RESULTS_FILE ST000866_AN001419_Results.txt UNITS:intensity #END