#METABOLOMICS WORKBENCH nmach_20180315_075909_mwtab.txt DATATRACK_ID:1344 STUDY_ID:ST000945 ANALYSIS_ID:AN001551 PROJECT_ID:PR000650 VERSION 1 CREATED_ON March 15, 2018, 1:44 pm #PROJECT PR:PROJECT_TITLE The gut microbiota influence the basal physiological parameters in equine PR:PROJECT_TITLE athletes PR:PROJECT_TYPE Comparison metabolome time PR:PROJECT_SUMMARY Comparison of serum metabolome and feces metagenome data from 52 horses before PR:PROJECT_SUMMARY and after an endurance competition. PR:INSTITUTE INRA PR:LAST_NAME Mach PR:FIRST_NAME Núria PR:ADDRESS Domaine Vilvert, 78352 Jouy en Josas, France PR:EMAIL nuria.mach@inra.fr PR:PHONE +33 (0)1 34 65 26 75 #STUDY ST:STUDY_TITLE The gut microbiota influence the basal physiological parameters in equine ST:STUDY_TITLE athletes ST:STUDY_SUMMARY Comparison of serum metabolome and feces metagenome data from 52 horses before ST:STUDY_SUMMARY and after an endurance competition. ST:INSTITUTE INRA ST:LAST_NAME Mach ST:FIRST_NAME Núria ST:ADDRESS Domaine de Vilvert, 78352 Jouy en Josas, France ST:EMAIL nuria.mach@inra.fr ST:PHONE +33 (0)1 34 65 26 75 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Equus caballus SU:TAXONOMY_ID 9796 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS ECAOMIC.201 ECAOMIC.201_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.201 ECAOMIC.201_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.202 ECAOMIC.202_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.202 ECAOMIC.202_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.203 ECAOMIC.203_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.203 ECAOMIC.203_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.204 ECAOMIC.204_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.204 ECAOMIC.204_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.205 ECAOMIC.205_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.205 ECAOMIC.205_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.206 ECAOMIC.206_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.206 ECAOMIC.206_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.209 ECAOMIC.209_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.209 ECAOMIC.209_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.210 ECAOMIC.210_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.210 ECAOMIC.210_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.211 ECAOMIC.211_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.211 ECAOMIC.211_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.212 ECAOMIC.212_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.212 ECAOMIC.212_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.213 ECAOMIC.213_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.213 ECAOMIC.213_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.214 ECAOMIC.214_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.214 ECAOMIC.214_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.215 ECAOMIC.215_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.215 ECAOMIC.215_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.216 ECAOMIC.216_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.216 ECAOMIC.216_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.217 ECAOMIC.217_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.217 ECAOMIC.217_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.218 ECAOMIC.218_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.218 ECAOMIC.218_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.220 ECAOMIC.220_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.220 ECAOMIC.220_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.221 ECAOMIC.221_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.221 ECAOMIC.221_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.222 ECAOMIC.222_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.222 ECAOMIC.222_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.224 ECAOMIC.224_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.224 ECAOMIC.224_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.226 ECAOMIC.226_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.226 ECAOMIC.226_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.227 ECAOMIC.227_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.227 ECAOMIC.227_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.228 ECAOMIC.228_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.228 ECAOMIC.228_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.229 ECAOMIC.229_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.229 ECAOMIC.229_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.231 ECAOMIC.231_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.231 ECAOMIC.231_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.232 ECAOMIC.232_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.232 ECAOMIC.232_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.233 ECAOMIC.233_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.233 ECAOMIC.233_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.234 ECAOMIC.234_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.234 ECAOMIC.234_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.235 ECAOMIC.235_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.235 ECAOMIC.235_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.236 ECAOMIC.236_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.236 ECAOMIC.236_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.237 ECAOMIC.237_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.237 ECAOMIC.237_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.238 ECAOMIC.238_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.238 ECAOMIC.238_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.239 ECAOMIC.239_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.239 ECAOMIC.239_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.241 ECAOMIC.241_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.241 ECAOMIC.241_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.242 ECAOMIC.242_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.242 ECAOMIC.242_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.243 ECAOMIC.243_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.243 ECAOMIC.243_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.244 ECAOMIC.244_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.244 ECAOMIC.244_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.245 ECAOMIC.245_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.245 ECAOMIC.245_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.246 ECAOMIC.246_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.246 ECAOMIC.246_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.247 ECAOMIC.247_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.247 ECAOMIC.247_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.248 ECAOMIC.248_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.248 ECAOMIC.248_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.249 ECAOMIC.249_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.249 ECAOMIC.249_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.250 ECAOMIC.250_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.250 ECAOMIC.250_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.251 ECAOMIC.251_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.251 ECAOMIC.251_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.253 ECAOMIC.253_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.253 ECAOMIC.253_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.254 ECAOMIC.254_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.254 ECAOMIC.254_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.255 ECAOMIC.255_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.255 ECAOMIC.255_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.256 ECAOMIC.256_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.256 ECAOMIC.256_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.257 ECAOMIC.257_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.257 ECAOMIC.257_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.259 ECAOMIC.259_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.259 ECAOMIC.259_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.260 ECAOMIC.260_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.260 ECAOMIC.260_T1 Time:T1 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.262 ECAOMIC.262_T0 Time:T0 Dataset=experimental SUBJECT_SAMPLE_FACTORS ECAOMIC.262 ECAOMIC.262_T1 Time:T1 Dataset=experimental #COLLECTION CO:COLLECTION_SUMMARY "Blood samples for metabolome pofiling were obtained from the jugular vein at CO:COLLECTION_SUMMARY rest (Basal, T0) and/or immediately after the end of the competition (T1). CO:COLLECTION_SUMMARY Pretreatment of the blood samples was carried out immediately after the CO:COLLECTION_SUMMARY collection because the access to refrigeration and electrical power supply was CO:COLLECTION_SUMMARY available under the field conditions. Briefly, whole blood samples from each CO:COLLECTION_SUMMARY horse were collected in sodium fluoride and oxalate tubes for metabolome CO:COLLECTION_SUMMARY profiling in order to inhibit further glycolysis that may increase the lactate CO:COLLECTION_SUMMARY levels after sampling. Whole blood draw for plasma generation was put at once at CO:COLLECTION_SUMMARY 4ºC to minimize the metabolic activity of cells and enzymes and kept the CO:COLLECTION_SUMMARY metabolite pattern almost stable. Clotting time at 4ºC was strictly controlled CO:COLLECTION_SUMMARY for all samples to avoid cell lyses and affect the components of the metabolome. CO:COLLECTION_SUMMARY After clotting at 4ºC, the plasma was separated from the blood cells, CO:COLLECTION_SUMMARY subsequently transported to the lab at 4ºC and frozen at -80 °C (no more CO:COLLECTION_SUMMARY than 5 h later, in all cases)." CO:SAMPLE_TYPE Blood (whole) #TREATMENT TR:TREATMENT_SUMMARY Two time course comparison of metabolome: T1 (post-endurance competition) TR:TREATMENT_SUMMARY relative to T0 (baseline, pre- endurance competition). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The plasmas were thawed at room temperature. In the 5 mm NMR tubes, 600 µL of SP:SAMPLEPREP_SUMMARY plasma was added with 100 µL deuterium oxide for field locking. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE - CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:ANALYSIS_TYPE NMR #NMR NM:INSTRUMENT_NAME Bruker Avance III NM:INSTRUMENT_TYPE FT-NMR NM:NMR_EXPERIMENT_TYPE 1D-1H NM:SPECTROMETER_FREQUENCY 500 NM:NMR_RESULTS_FILE ST000945_AN001551_Results.txt UNITS:ppm #END