#METABOLOMICS WORKBENCH hormel101_20180409_155755 DATATRACK_ID:1361 STUDY_ID:ST000948 ANALYSIS_ID:AN001557 PROJECT_ID:PR000652
VERSION             	1
CREATED_ON             	April 10, 2018, 3:05 pm
#PROJECT
PR:PROJECT_TITLE                 	Mayo Pilot and Feasibility: Mechanisms for Insulin Resistance in Polycystic
PR:PROJECT_TITLE                 	Ovary Syndrome
PR:PROJECT_SUMMARY               	Polycystic Ovary Syndrome (PCOS), a condition of androgen excess, infrequent
PR:PROJECT_SUMMARY               	ovulation, and insulin resistance is the most common endocrine disorder among
PR:PROJECT_SUMMARY               	premenopausal women. Little is known about the exact mechanisms of insulin
PR:PROJECT_SUMMARY               	resistance in PCOS and how metformin can improve insulin sensitivity, increase
PR:PROJECT_SUMMARY               	the frequency of ovulation and lower androgens in PCOS. Preliminary data from
PR:PROJECT_SUMMARY               	metabolomic analyses of amino acids demonstrate increased concentrations of
PR:PROJECT_SUMMARY               	lysine and its metabolite, α-aminoadipic acid (AAA), in PCOS versus obese
PR:PROJECT_SUMMARY               	controls. Interestingly, greater AAA concentrations predicted the development of
PR:PROJECT_SUMMARY               	type 2 diabetes in the Framingham epidemiologic cohort, experimentally lowers
PR:PROJECT_SUMMARY               	glucose in animal models and increases insulin secretion in vitro. To date, the
PR:PROJECT_SUMMARY               	mechanism for increased circulating concentrations of lysine and AAA in
PR:PROJECT_SUMMARY               	insulin-resistant individuals is not known. Building upon these findings, we
PR:PROJECT_SUMMARY               	have initiated a project to simultaneously study lysine and AAA kinetics for the
PR:PROJECT_SUMMARY               	first time in insulin-resistant individuals using stable isotope tracer
PR:PROJECT_SUMMARY               	methodology. We will evaluate: 1) whether lysine and AAA kinetics are altered in
PR:PROJECT_SUMMARY               	PCOS versus healthy controls; 2) the effect of hyperinsulinemia on lysine and
PR:PROJECT_SUMMARY               	AAA kinetics in PCOS versus controls; 3) whether treatment to improve insulin
PR:PROJECT_SUMMARY               	sensitivity changes lysine and AAA kinetics in PCOS. The long-term goal is to
PR:PROJECT_SUMMARY               	target pathways for the treatment of PCOS and the prevention of type 2 diabetes
PR:PROJECT_SUMMARY               	in PCOS and other insulin-resistant individuals at greater risk for type 2
PR:PROJECT_SUMMARY               	diabetes.
PR:INSTITUTE                     	Mayo Clinic
PR:LAST_NAME                     	Chang
PR:FIRST_NAME                    	Alice
PR:ADDRESS                       	200 First St. SW, Rochester, Minnesota, 55905, USA
PR:EMAIL                         	Chang.Alice1@mayo.edu
PR:PHONE                         	507-286-0505
#STUDY
ST:STUDY_TITLE                   	Mechanisms for Insulin Resistance in Polycystic Ovary Syndrome: aminoadipic
ST:STUDY_TITLE                   	acid, lysine concentrations, and enrichment (part II)
ST:STUDY_SUMMARY                 	"To determine how metformin therapy changes lysine and AAA kinetics in PCOS and
ST:STUDY_SUMMARY                 	whether this is associated with improvements in insulin sensitivity. Changes in
ST:STUDY_SUMMARY                 	lysine and AAA flux before and after three months of metformin therapy will be
ST:STUDY_SUMMARY                 	compared to women with PCOS randomized to no treatment for three months.
ST:INSTITUTE                     	Mayo Clinic
ST:LAST_NAME                     	Chang
ST:FIRST_NAME                    	Alice
ST:ADDRESS                       	200 First St. SW, Rochester, Minnesota, 55905, USA
ST:EMAIL                         	Chang.Alice1@mayo.edu
ST:PHONE                         	507-286-0505
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-12	Time point:baseline | group:PCOS	visit=1; Date of study=5/12/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-13	Time point:-60 | group:PCOS	visit=1; Date of study=5/13/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-14	Time point:-45 | group:PCOS	visit=1; Date of study=5/13/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-15	Time point:-30 | group:PCOS	visit=1; Date of study=5/13/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-16	Time point:-15 | group:PCOS	visit=1; Date of study=5/13/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-17	Time point:60 | group:PCOS	visit=1; Date of study=5/13/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-18	Time point:120 | group:PCOS	visit=1; Date of study=5/13/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-19	Time point:135 | group:PCOS	visit=1; Date of study=5/13/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-20	Time point:150 | group:PCOS	visit=1; Date of study=5/13/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-21	Time point:165 | group:PCOS	visit=1; Date of study=5/13/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-22	Time point:180 | group:PCOS	visit=1; Date of study=5/13/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-12	Time point:baseline | group:PCOS	visit=1; Date of study=5/9/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-13	Time point:-60 | group:PCOS	visit=1; Date of study=5/10/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-14	Time point:-45 | group:PCOS	visit=1; Date of study=5/10/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-15	Time point:-30 | group:PCOS	visit=1; Date of study=5/10/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-16	Time point:-15 | group:PCOS	visit=1; Date of study=5/10/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-17	Time point:60 | group:PCOS	visit=1; Date of study=5/10/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-18	Time point:120 | group:PCOS	visit=1; Date of study=5/10/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-19	Time point:135 | group:PCOS	visit=1; Date of study=5/10/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-20	Time point:150 | group:PCOS	visit=1; Date of study=5/10/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-21	Time point:165 | group:PCOS	visit=1; Date of study=5/10/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-22	Time point:180 | group:PCOS	visit=1; Date of study=5/10/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-12	Time point:baseline | group:PCOS	visit=1; Date of study=4/5/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-13	Time point:-60 | group:PCOS	visit=1; Date of study=4/6/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-14	Time point:-45 | group:PCOS	visit=1; Date of study=4/6/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-15	Time point:-30 | group:PCOS	visit=1; Date of study=4/6/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-16	Time point:-15 | group:PCOS	visit=1; Date of study=4/6/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-17	Time point:60 | group:PCOS	visit=1; Date of study=4/6/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-18	Time point:120 | group:PCOS	visit=1; Date of study=4/6/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-19	Time point:135 | group:PCOS	visit=1; Date of study=4/6/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-20	Time point:150 | group:PCOS	visit=1; Date of study=4/6/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-21	Time point:165 | group:PCOS	visit=1; Date of study=4/6/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-22	Time point:180 | group:PCOS	visit=1; Date of study=4/6/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-12	Time point:baseline | group:PCOS	visit=1; Date of study=5/4/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-13	Time point:-60 | group:PCOS	visit=1; Date of study=5/5/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-14	Time point:-45 | group:PCOS	visit=1; Date of study=5/5/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-15	Time point:-30 | group:PCOS	visit=1; Date of study=5/5/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-16	Time point:-15 | group:PCOS	visit=1; Date of study=5/5/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-17	Time point:60 | group:PCOS	visit=1; Date of study=5/5/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-18	Time point:120 | group:PCOS	visit=1; Date of study=5/5/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-19	Time point:135 | group:PCOS	visit=1; Date of study=5/5/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-20	Time point:150 | group:PCOS	visit=1; Date of study=5/5/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-21	Time point:165 | group:PCOS	visit=1; Date of study=5/5/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-22	Time point:180 | group:PCOS	visit=1; Date of study=5/5/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-1	Time point:baseline | group:PCOS	visit=2; Date of study=9/8/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-2	Time point:-60 | group:PCOS	visit=2; Date of study=9/8/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-3	Time point:-45 | group:PCOS	visit=2; Date of study=9/8/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-4	Time point:-30 | group:PCOS	visit=2; Date of study=9/8/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-5	Time point:-15 | group:PCOS	visit=2; Date of study=9/8/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-6	Time point:60 | group:PCOS	visit=2; Date of study=9/8/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-7	Time point:120 | group:PCOS	visit=2; Date of study=9/8/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-8	Time point:135 | group:PCOS	visit=2; Date of study=9/8/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-9	Time point:150 | group:PCOS	visit=2; Date of study=9/8/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-10	Time point:165 | group:PCOS	visit=2; Date of study=9/8/2016
SUBJECT_SAMPLE_FACTORS           	9980	ms6436-11	Time point:180 | group:PCOS	visit=2; Date of study=9/8/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-1	Time point:baseline | group:PCOS	visit=2; Date of study=8/22/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-2	Time point:-60 | group:PCOS	visit=2; Date of study=8/22/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-3	Time point:-45 | group:PCOS	visit=2; Date of study=8/22/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-4	Time point:-30 | group:PCOS	visit=2; Date of study=8/22/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-5	Time point:-15 | group:PCOS	visit=2; Date of study=8/22/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-6	Time point:60 | group:PCOS	visit=2; Date of study=8/22/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-7	Time point:120 | group:PCOS	visit=2; Date of study=8/22/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-8	Time point:135 | group:PCOS	visit=2; Date of study=8/22/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-9	Time point:150 | group:PCOS	visit=2; Date of study=8/22/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-10	Time point:165 | group:PCOS	visit=2; Date of study=8/22/2016
SUBJECT_SAMPLE_FACTORS           	2218	ms6437-11	Time point:180 | group:PCOS	visit=2; Date of study=8/22/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-1	Time point:baseline | group:PCOS	visit=2; Date of study=7/13/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-2	Time point:-60 | group:PCOS	visit=2; Date of study=7/13/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-3	Time point:-45 | group:PCOS	visit=2; Date of study=7/13/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-4	Time point:-30 | group:PCOS	visit=2; Date of study=7/13/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-5	Time point:-15 | group:PCOS	visit=2; Date of study=7/13/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-6	Time point:60 | group:PCOS	visit=2; Date of study=7/13/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-7	Time point:120 | group:PCOS	visit=2; Date of study=7/13/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-8	Time point:135 | group:PCOS	visit=2; Date of study=7/13/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-9	Time point:150 | group:PCOS	visit=2; Date of study=7/13/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-10	Time point:165 | group:PCOS	visit=2; Date of study=7/13/2016
SUBJECT_SAMPLE_FACTORS           	1228	ms6438-11	Time point:180 | group:PCOS	visit=2; Date of study=7/13/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-1	Time point:baseline | group:PCOS	visit=2; Date of study=8/4/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-2	Time point:-60 | group:PCOS	visit=2; Date of study=8/4/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-3	Time point:-45 | group:PCOS	visit=2; Date of study=8/4/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-4	Time point:-30 | group:PCOS	visit=2; Date of study=8/4/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-5	Time point:-15 | group:PCOS	visit=2; Date of study=8/4/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-6	Time point:60 | group:PCOS	visit=2; Date of study=8/4/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-7	Time point:120 | group:PCOS	visit=2; Date of study=8/4/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-8	Time point:135 | group:PCOS	visit=2; Date of study=8/4/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-9	Time point:150 | group:PCOS	visit=2; Date of study=8/4/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-10	Time point:165 | group:PCOS	visit=2; Date of study=8/4/2016
SUBJECT_SAMPLE_FACTORS           	4434	ms6439-11	Time point:180 | group:PCOS	visit=2; Date of study=8/4/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-12	Time point:baseline | group:PCOS	visit=1; Date of study=5/3/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-13	Time point:-60 | group:PCOS	visit=1; Date of study=5/4/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-14	Time point:-45 | group:PCOS	visit=1; Date of study=5/4/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-15	Time point:-30 | group:PCOS	visit=1; Date of study=5/4/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-16	Time point:-15 | group:PCOS	visit=1; Date of study=5/4/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-17	Time point:60 | group:PCOS	visit=1; Date of study=5/4/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-18	Time point:120 | group:PCOS	visit=1; Date of study=5/4/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-19	Time point:135 | group:PCOS	visit=1; Date of study=5/4/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-20	Time point:150 | group:PCOS	visit=1; Date of study=5/4/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-21	Time point:165 | group:PCOS	visit=1; Date of study=5/4/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-22	Time point:180 | group:PCOS	visit=1; Date of study=5/4/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-12	Time point:baseline | group:PCOS	visit=1; Date of study=3/10/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-13	Time point:-60 | group:PCOS	visit=1; Date of study=3/11/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-14	Time point:-45 | group:PCOS	visit=1; Date of study=3/11/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-15	Time point:-30 | group:PCOS	visit=1; Date of study=3/11/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-16	Time point:-15 | group:PCOS	visit=1; Date of study=3/11/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-17	Time point:60 | group:PCOS	visit=1; Date of study=3/11/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-18	Time point:120 | group:PCOS	visit=1; Date of study=3/11/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-19	Time point:135 | group:PCOS	visit=1; Date of study=3/11/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-20	Time point:150 | group:PCOS	visit=1; Date of study=3/11/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-21	Time point:165 | group:PCOS	visit=1; Date of study=3/11/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-22	Time point:180 | group:PCOS	visit=1; Date of study=3/11/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-12	Time point:baseline | group:PCOS	visit=1; Date of study=4/21/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-13	Time point:-60 | group:PCOS	visit=1; Date of study=4/22/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-14	Time point:-45 | group:PCOS	visit=1; Date of study=4/22/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-15	Time point:-30 | group:PCOS	visit=1; Date of study=4/22/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-16	Time point:-15 | group:PCOS	visit=1; Date of study=4/22/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-17	Time point:60 | group:PCOS	visit=1; Date of study=4/22/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-18	Time point:120 | group:PCOS	visit=1; Date of study=4/22/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-19	Time point:135 | group:PCOS	visit=1; Date of study=4/22/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-20	Time point:150 | group:PCOS	visit=1; Date of study=4/22/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-21	Time point:165 | group:PCOS	visit=1; Date of study=4/22/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-22	Time point:180 | group:PCOS	visit=1; Date of study=4/22/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-12	Time point:baseline | group:PCOS	visit=1; Date of study=3/29/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-13	Time point:-60 | group:PCOS	visit=1; Date of study=3/30/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-14	Time point:-45 | group:PCOS	visit=1; Date of study=3/30/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-15	Time point:-30 | group:PCOS	visit=1; Date of study=3/30/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-16	Time point:-15 | group:PCOS	visit=1; Date of study=3/30/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-17	Time point:60 | group:PCOS	visit=1; Date of study=3/30/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-18	Time point:120 | group:PCOS	visit=1; Date of study=3/30/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-19	Time point:135 | group:PCOS	visit=1; Date of study=3/30/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-20	Time point:150 | group:PCOS	visit=1; Date of study=3/30/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-21	Time point:165 | group:PCOS	visit=1; Date of study=3/30/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-22	Time point:180 | group:PCOS	visit=1; Date of study=3/30/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-1	Time point:baseline | group:PCOS	visit=2; Date of study=7/18/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-2	Time point:-60 | group:PCOS	visit=2; Date of study=7/18/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-3	Time point:-45 | group:PCOS	visit=2; Date of study=7/18/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-4	Time point:-30 | group:PCOS	visit=2; Date of study=7/18/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-5	Time point:-15 | group:PCOS	visit=2; Date of study=7/18/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-6	Time point:60 | group:PCOS	visit=2; Date of study=7/18/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-7	Time point:120 | group:PCOS	visit=2; Date of study=7/18/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-8	Time point:135 | group:PCOS	visit=2; Date of study=7/18/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-9	Time point:150 | group:PCOS	visit=2; Date of study=7/18/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-10	Time point:165 | group:PCOS	visit=2; Date of study=7/18/2016
SUBJECT_SAMPLE_FACTORS           	5908	ms6440-11	Time point:180 | group:PCOS	visit=2; Date of study=7/18/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-1	Time point:baseline | group:PCOS	visit=2; Date of study=7/12/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-2	Time point:-60 | group:PCOS	visit=2; Date of study=7/12/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-3	Time point:-45 | group:PCOS	visit=2; Date of study=7/12/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-4	Time point:-30 | group:PCOS	visit=2; Date of study=7/12/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-5	Time point:-15 | group:PCOS	visit=2; Date of study=7/12/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-6	Time point:60 | group:PCOS	visit=2; Date of study=7/12/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-7	Time point:120 | group:PCOS	visit=2; Date of study=7/12/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-8	Time point:135 | group:PCOS	visit=2; Date of study=7/12/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-9	Time point:150 | group:PCOS	visit=2; Date of study=7/12/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-10	Time point:165 | group:PCOS	visit=2; Date of study=7/12/2016
SUBJECT_SAMPLE_FACTORS           	9931	ms6441-11	Time point:180 | group:PCOS	visit=2; Date of study=7/12/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-1	Time point:baseline | group:PCOS	visit=2; Date of study=8/12/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-2	Time point:-60 | group:PCOS	visit=2; Date of study=8/12/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-3	Time point:-45 | group:PCOS	visit=2; Date of study=8/12/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-4	Time point:-30 | group:PCOS	visit=2; Date of study=8/12/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-5	Time point:-15 | group:PCOS	visit=2; Date of study=8/12/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-6	Time point:60 | group:PCOS	visit=2; Date of study=8/12/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-7	Time point:120 | group:PCOS	visit=2; Date of study=8/12/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-8	Time point:135 | group:PCOS	visit=2; Date of study=8/12/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-9	Time point:150 | group:PCOS	visit=2; Date of study=8/12/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-10	Time point:165 | group:PCOS	visit=2; Date of study=8/12/2016
SUBJECT_SAMPLE_FACTORS           	4620	ms6442-11	Time point:180 | group:PCOS	visit=2; Date of study=8/12/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-1	Time point:baseline | group:PCOS	visit=2; Date of study=7/11/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-2	Time point:-60 | group:PCOS	visit=2; Date of study=7/11/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-3	Time point:-45 | group:PCOS	visit=2; Date of study=7/11/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-4	Time point:-30 | group:PCOS	visit=2; Date of study=7/11/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-5	Time point:-15 | group:PCOS	visit=2; Date of study=7/11/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-6	Time point:60 | group:PCOS	visit=2; Date of study=7/11/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-7	Time point:120 | group:PCOS	visit=2; Date of study=7/11/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-8	Time point:135 | group:PCOS	visit=2; Date of study=7/11/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-9	Time point:150 | group:PCOS	visit=2; Date of study=7/11/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-10	Time point:165 | group:PCOS	visit=2; Date of study=7/11/2016
SUBJECT_SAMPLE_FACTORS           	8404	ms6443-11	Time point:180 | group:PCOS	visit=2; Date of study=7/11/2016
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-1	Time point:baseline | group:PCOS	visit=1; Date of study=12/2/2015
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-2	Time point:-60 | group:PCOS	visit=1; Date of study=12/2/2015
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-3	Time point:-45 | group:PCOS	visit=1; Date of study=12/2/2015
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-4	Time point:-30 | group:PCOS	visit=1; Date of study=12/2/2015
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-5	Time point:-15 | group:PCOS	visit=1; Date of study=12/2/2015
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-6	Time point:60 | group:PCOS	visit=1; Date of study=12/2/2015
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-7	Time point:120 | group:PCOS	visit=1; Date of study=12/2/2015
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-8	Time point:135 | group:PCOS	visit=1; Date of study=12/2/2015
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-9	Time point:150 | group:PCOS	visit=1; Date of study=12/2/2015
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-10	Time point:165 | group:PCOS	visit=1; Date of study=12/2/2015
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-11	Time point:180 | group:PCOS	visit=1; Date of study=12/2/2015
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-12	Time point:baseline | group:PCOS	visit=2; Date of study=3/16/2016
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-13	Time point:-60 | group:PCOS	visit=2; Date of study=3/16/2016
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-14	Time point:-45 | group:PCOS	visit=2; Date of study=3/16/2016
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-15	Time point:-30 | group:PCOS	visit=2; Date of study=3/16/2016
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-16	Time point:-15 | group:PCOS	visit=2; Date of study=3/16/2016
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-17	Time point:60 | group:PCOS	visit=2; Date of study=3/16/2016
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-18	Time point:120 | group:PCOS	visit=2; Date of study=3/16/2016
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-19	Time point:135 | group:PCOS	visit=2; Date of study=3/16/2016
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-20	Time point:150 | group:PCOS	visit=2; Date of study=3/16/2016
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-21	Time point:165 | group:PCOS	visit=2; Date of study=3/16/2016
SUBJECT_SAMPLE_FACTORS           	3463	ms6712-22	Time point:180 | group:PCOS	visit=2; Date of study=3/16/2016
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-1	Time point:baseline | group:PCOS	visit=1; Date of study=12/19/2015
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-2	Time point:-60 | group:PCOS	visit=1; Date of study=12/19/2015
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-3	Time point:-45 | group:PCOS	visit=1; Date of study=12/19/2015
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-4	Time point:-30 | group:PCOS	visit=1; Date of study=12/19/2015
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-5	Time point:-15 | group:PCOS	visit=1; Date of study=12/19/2015
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-6	Time point:60 | group:PCOS	visit=1; Date of study=12/19/2015
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-7	Time point:120 | group:PCOS	visit=1; Date of study=12/19/2015
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-8	Time point:135 | group:PCOS	visit=1; Date of study=12/19/2015
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-9	Time point:150 | group:PCOS	visit=1; Date of study=12/19/2015
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-10	Time point:165 | group:PCOS	visit=1; Date of study=12/19/2015
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-11	Time point:180 | group:PCOS	visit=1; Date of study=12/19/2015
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-12	Time point:baseline | group:PCOS	visit=2; Date of study=4/9/2016
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-13	Time point:-60 | group:PCOS	visit=2; Date of study=4/9/2016
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-14	Time point:-45 | group:PCOS	visit=2; Date of study=4/9/2016
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-15	Time point:-30 | group:PCOS	visit=2; Date of study=4/9/2016
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-16	Time point:-15 | group:PCOS	visit=2; Date of study=4/9/2016
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-17	Time point:60 | group:PCOS	visit=2; Date of study=4/9/2016
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-18	Time point:120 | group:PCOS	visit=2; Date of study=4/9/2016
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-19	Time point:135 | group:PCOS	visit=2; Date of study=4/9/2016
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-20	Time point:150 | group:PCOS	visit=2; Date of study=4/9/2016
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-21	Time point:165 | group:PCOS	visit=2; Date of study=4/9/2016
SUBJECT_SAMPLE_FACTORS           	7182	ms6713-22	Time point:180 | group:PCOS	visit=2; Date of study=4/9/2016
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-1	Time point:baseline | group:PCOS	visit=1; Date of study=12/16/2015
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-2	Time point:-60 | group:PCOS	visit=1; Date of study=12/16/2015
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-3	Time point:-45 | group:PCOS	visit=1; Date of study=12/16/2015
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-4	Time point:-30 | group:PCOS	visit=1; Date of study=12/16/2015
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-5	Time point:-15 | group:PCOS	visit=1; Date of study=12/16/2015
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-6	Time point:60 | group:PCOS	visit=1; Date of study=12/16/2015
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-7	Time point:120 | group:PCOS	visit=1; Date of study=12/16/2015
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-8	Time point:135 | group:PCOS	visit=1; Date of study=12/16/2015
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-9	Time point:150 | group:PCOS	visit=1; Date of study=12/16/2015
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-10	Time point:165 | group:PCOS	visit=1; Date of study=12/16/2015
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-11	Time point:180 | group:PCOS	visit=1; Date of study=12/16/2015
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-12	Time point:baseline | group:PCOS	visit=2; Date of study=3/25/2016
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-13	Time point:-60 | group:PCOS	visit=2; Date of study=3/25/2016
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-14	Time point:-45 | group:PCOS	visit=2; Date of study=3/25/2016
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-15	Time point:-30 | group:PCOS	visit=2; Date of study=3/25/2016
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-16	Time point:-15 | group:PCOS	visit=2; Date of study=3/25/2016
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-17	Time point:60 | group:PCOS	visit=2; Date of study=3/25/2016
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-18	Time point:120 | group:PCOS	visit=2; Date of study=3/25/2016
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-19	Time point:135 | group:PCOS	visit=2; Date of study=3/25/2016
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-20	Time point:150 | group:PCOS	visit=2; Date of study=3/25/2016
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-21	Time point:165 | group:PCOS	visit=2; Date of study=3/25/2016
SUBJECT_SAMPLE_FACTORS           	8999	ms6714-22	Time point:180 | group:PCOS	visit=2; Date of study=3/25/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-1	Time point:baseline | group:PCOS	visit=1; Date of study=7/14/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-2	Time point:-60 | group:PCOS	visit=1; Date of study=7/14/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-3	Time point:-45 | group:PCOS	visit=1; Date of study=7/14/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-4	Time point:-30 | group:PCOS	visit=1; Date of study=7/14/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-5	Time point:-15 | group:PCOS	visit=1; Date of study=7/14/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-6	Time point:60 | group:PCOS	visit=1; Date of study=7/14/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-7	Time point:120 | group:PCOS	visit=1; Date of study=7/14/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-8	Time point:135 | group:PCOS	visit=1; Date of study=7/14/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-9	Time point:150 | group:PCOS	visit=1; Date of study=7/14/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-10	Time point:165 | group:PCOS	visit=1; Date of study=7/14/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-11	Time point:180 | group:PCOS	visit=1; Date of study=7/14/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-12	Time point:baseline | group:PCOS	visit=2; Date of study=10/21/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-13	Time point:-60 | group:PCOS	visit=2; Date of study=10/21/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-14	Time point:-45 | group:PCOS	visit=2; Date of study=10/21/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-15	Time point:-30 | group:PCOS	visit=2; Date of study=10/21/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-16	Time point:-15 | group:PCOS	visit=2; Date of study=10/21/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-17	Time point:60 | group:PCOS	visit=2; Date of study=10/21/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-18	Time point:120 | group:PCOS	visit=2; Date of study=10/21/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-19	Time point:135 | group:PCOS	visit=2; Date of study=10/21/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-20	Time point:150 | group:PCOS	visit=2; Date of study=10/21/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-21	Time point:165 | group:PCOS	visit=2; Date of study=10/21/2016
SUBJECT_SAMPLE_FACTORS           	7725	ms6715-22	Time point:180 | group:PCOS	visit=2; Date of study=10/21/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-1	Time point:baseline | group:PCOS	visit=1; Date of study=1/13/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-2	Time point:-60 | group:PCOS	visit=1; Date of study=1/13/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-3	Time point:-45 | group:PCOS	visit=1; Date of study=1/13/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-4	Time point:-30 | group:PCOS	visit=1; Date of study=1/13/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-5	Time point:-15 | group:PCOS	visit=1; Date of study=1/13/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-6	Time point:60 | group:PCOS	visit=1; Date of study=1/13/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-7	Time point:120 | group:PCOS	visit=1; Date of study=1/13/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-8	Time point:135 | group:PCOS	visit=1; Date of study=1/13/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-9	Time point:150 | group:PCOS	visit=1; Date of study=1/13/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-10	Time point:165 | group:PCOS	visit=1; Date of study=1/13/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-11	Time point:180 | group:PCOS	visit=1; Date of study=1/13/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-12	Time point:baseline | group:PCOS	visit=2; Date of study=4/30/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-13	Time point:-60 | group:PCOS	visit=2; Date of study=4/30/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-14	Time point:-45 | group:PCOS	visit=2; Date of study=4/30/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-15	Time point:-30 | group:PCOS	visit=2; Date of study=4/30/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-16	Time point:-15 | group:PCOS	visit=2; Date of study=4/30/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-17	Time point:60 | group:PCOS	visit=2; Date of study=4/30/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-18	Time point:120 | group:PCOS	visit=2; Date of study=4/30/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-19	Time point:135 | group:PCOS	visit=2; Date of study=4/30/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-20	Time point:150 | group:PCOS	visit=2; Date of study=4/30/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-21	Time point:165 | group:PCOS	visit=2; Date of study=4/30/2016
SUBJECT_SAMPLE_FACTORS           	3635	ms6716-22	Time point:180 | group:PCOS	visit=2; Date of study=4/30/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-1	Time point:baseline | group:PCOS	visit=1; Date of study=8/1/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-2	Time point:-60 | group:PCOS	visit=1; Date of study=8/1/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-3	Time point:-45 | group:PCOS	visit=1; Date of study=8/1/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-4	Time point:-30 | group:PCOS	visit=1; Date of study=8/1/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-5	Time point:-15 | group:PCOS	visit=1; Date of study=8/1/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-6	Time point:60 | group:PCOS	visit=1; Date of study=8/1/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-7	Time point:120 | group:PCOS	visit=1; Date of study=8/1/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-8	Time point:135 | group:PCOS	visit=1; Date of study=8/1/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-9	Time point:150 | group:PCOS	visit=1; Date of study=8/1/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-10	Time point:165 | group:PCOS	visit=1; Date of study=8/1/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-11	Time point:180 | group:PCOS	visit=1; Date of study=8/1/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-12	Time point:baseline | group:PCOS	visit=2; Date of study=11/14/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-13	Time point:-60 | group:PCOS	visit=2; Date of study=11/14/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-14	Time point:-45 | group:PCOS	visit=2; Date of study=11/14/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-15	Time point:-30 | group:PCOS	visit=2; Date of study=11/14/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-16	Time point:-15 | group:PCOS	visit=2; Date of study=11/14/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-17	Time point:60 | group:PCOS	visit=2; Date of study=11/14/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-18	Time point:120 | group:PCOS	visit=2; Date of study=11/14/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-19	Time point:135 | group:PCOS	visit=2; Date of study=11/14/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-20	Time point:150 | group:PCOS	visit=2; Date of study=11/14/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-21	Time point:165 | group:PCOS	visit=2; Date of study=11/14/2016
SUBJECT_SAMPLE_FACTORS           	9103	ms6717-22	Time point:180 | group:PCOS	visit=2; Date of study=11/14/2016
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-1	Time point:baseline | group:PCOS	visit=1; Date of study=7/28/2016
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-2	Time point:-60 | group:PCOS	visit=1; Date of study=7/28/2016
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-3	Time point:-45 | group:PCOS	visit=1; Date of study=7/28/2016
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-4	Time point:-30 | group:PCOS	visit=1; Date of study=7/28/2016
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-5	Time point:-15 | group:PCOS	visit=1; Date of study=7/28/2016
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-6	Time point:60 | group:PCOS	visit=1; Date of study=7/28/2016
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-7	Time point:120 | group:PCOS	visit=1; Date of study=7/28/2016
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-8	Time point:135 | group:PCOS	visit=1; Date of study=7/28/2016
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-9	Time point:150 | group:PCOS	visit=1; Date of study=7/28/2016
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-10	Time point:165 | group:PCOS	visit=1; Date of study=7/28/2016
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-11	Time point:180 | group:PCOS	visit=1; Date of study=42579
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-12	Time point:baseline | group:PCOS	visit=2; Date of study=42677
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-13	Time point:-60 | group:PCOS	visit=2; Date of study=42677
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-14	Time point:-45 | group:PCOS	visit=2; Date of study=42677
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-15	Time point:-30 | group:PCOS	visit=2; Date of study=42677
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-16	Time point:-15 | group:PCOS	visit=2; Date of study=42677
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-17	Time point:60 | group:PCOS	visit=2; Date of study=42677
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-18	Time point:120 | group:PCOS	visit=2; Date of study=42677
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-19	Time point:135 | group:PCOS	visit=2; Date of study=42677
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-20	Time point:150 | group:PCOS	visit=2; Date of study=42677
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-21	Time point:165 | group:PCOS	visit=2; Date of study=42677
SUBJECT_SAMPLE_FACTORS           	6936	ms6718-22	Time point:180 | group:PCOS	visit=2; Date of study=42677
#COLLECTION
CO:COLLECTION_SUMMARY            	"We will perform a hyperinsulinemic-euglycemic clamp as previously described
CO:COLLECTION_SUMMARY            	except that in this current proposal we will perform the study for 3 hours.(36)
CO:COLLECTION_SUMMARY            	The goal for this infusion is to assess the effect of hyperinsulinemia on lysine
CO:COLLECTION_SUMMARY            	and AAA kinetics and whether metformin changes the effect of hyperinsulinemia.
CO:COLLECTION_SUMMARY            	We will collect samples every 10 min for glucose. 40% dextrose will be infused
CO:COLLECTION_SUMMARY            	at a variable rate during the clamp to maintain euglycemia.(36) During the last
CO:COLLECTION_SUMMARY            	hour of the clamp, 5 blood samples will be drawn for measurement of stable
CO:COLLECTION_SUMMARY            	isotope tracer enrichment and amino acid concentrations. Previous studies have
CO:COLLECTION_SUMMARY            	established that the combination of metformin and pioglitazone decrease lysine
CO:COLLECTION_SUMMARY            	and AAA concentrations in overweight and obese adults with impaired fasting
CO:COLLECTION_SUMMARY            	glucose or untreated diabetes.(7) Aim 3 is designed to establish whether changes
CO:COLLECTION_SUMMARY            	in lysine and AAA flux are associated with changes in insulin sensitivity.
CO:COLLECTION_SUMMARY            	Measurement of lysine and AAA flux will be repeated in 10 women with PCOS after
CO:COLLECTION_SUMMARY            	three months of metformin and 10 women with PCOS randomized to receive no
CO:COLLECTION_SUMMARY            	treatment for three months. Insulin sensitivity and other measures of glucose
CO:COLLECTION_SUMMARY            	metabolism will be calculated from an oral glucose tolerance test using the oral
CO:COLLECTION_SUMMARY            	minimal model as described below."
CO:SAMPLE_TYPE                   	Blood (plasma)
#TREATMENT
TR:TREATMENT_SUMMARY             	"Ten women with PCOS will receive metformin therapy, and ten women will be
TR:TREATMENT_SUMMARY             	randomized to receive no therapy and undergo the same repeat visits after 3
TR:TREATMENT_SUMMARY             	months. Ten age-matched women without PCOS with a BMI < 25 will be recruited as
TR:TREATMENT_SUMMARY             	the control group for the baseline visit. Aside from criteria that they do not
TR:TREATMENT_SUMMARY             	have PCOS and have a BMI < 25, this control group will have the same inclusion
TR:TREATMENT_SUMMARY             	and exclusion criteria below. They will not receive metformin and will not
TR:TREATMENT_SUMMARY             	return for repeat visits after 3 months. Metformin therapy: Previous studies
TR:TREATMENT_SUMMARY             	with metformin demonstrated improvement in insulin sensitivity as early as 3
TR:TREATMENT_SUMMARY             	months with 1000 mg daily.(5, 33, 34) Metformin will be initiated with 500 mg
TR:TREATMENT_SUMMARY             	extended-release tablet daily for one week, 1000 mg daily for one week and then
TR:TREATMENT_SUMMARY             	1500 mg daily. Visits 4 and 5 will be conducted three months after full dose is
TR:TREATMENT_SUMMARY             	achieved. Oral glucose tolerance test: After 2 baseline fasting samples, 75 g of
TR:TREATMENT_SUMMARY             	oral dextrose will be ingested with blood samples will be drawn at 10’, 20’,
TR:TREATMENT_SUMMARY             	30’, 60’, 90’, 120’, 150’and 180’ for measurement of glucose,
TR:TREATMENT_SUMMARY             	insulin, c-peptide. Insulin sensitivity will be calculated using the oral
TR:TREATMENT_SUMMARY             	glucose minimal model. Stable isotope tracer infusions (Figure 5): Three days
TR:TREATMENT_SUMMARY             	prior to the tracer study, the participants will be placed on a
TR:TREATMENT_SUMMARY             	weight-maintaining diet consisting of 50% carbohydrates, 20% protein, and 30%
TR:TREATMENT_SUMMARY             	fats. Fat free mass (FFM) measured by dual-energy x-ray absorptiometry will be
TR:TREATMENT_SUMMARY             	used for dose calculations of the stable isotope tracers and insulin infusions
TR:TREATMENT_SUMMARY             	for the hyperinsulinemic-euglycemic clamp. A priming bolus dose of
TR:TREATMENT_SUMMARY             	L-[α-15N]-lysine, 3 to 5 μmol/kg FFM, will be given at the start of a 3 hour
TR:TREATMENT_SUMMARY             	infusion of 3 to 5 μmol/kg FFM/hr to achieve a plateau as previously
TR:TREATMENT_SUMMARY             	described.(29) At the same time, a priming bolus of 1 to 2 μmol/kg FFM
TR:TREATMENT_SUMMARY             	L-[1-13C]-2-aminoadipic acid will be given followed by infusion of 1 to 2
TR:TREATMENT_SUMMARY             	μmol/kg/hr based on prior study.(30) A retrograde hand intravenous line will be
TR:TREATMENT_SUMMARY             	placed with the hand placed in a warm box maintained at 140°F to obtain
TR:TREATMENT_SUMMARY             	arterialized venous blood samples for measurement of lysine and AAA
TR:TREATMENT_SUMMARY             	concentrations and stable isotopic enrichment at steady state and during the
TR:TREATMENT_SUMMARY             	clamp. Hyperinsulinemic-euglycemic clamp: We will perform a
TR:TREATMENT_SUMMARY             	hyperinsulinemic-euglycemic clamp as previously described except that in this
TR:TREATMENT_SUMMARY             	current proposal we will perform the study for 3 hours.(36) The goal for this
TR:TREATMENT_SUMMARY             	infusion is to assess the effect of hyperinsulinemia on lysine and AAA kinetics
TR:TREATMENT_SUMMARY             	and whether metformin changes the effect of hyperinsulinemia. We will collect
TR:TREATMENT_SUMMARY             	samples every 10 min for glucose. 40% dextrose will be infused at a variable
TR:TREATMENT_SUMMARY             	rate during the clamp to maintain euglycemia.(36) During the last hour of the
TR:TREATMENT_SUMMARY             	clamp, 5 blood samples will be drawn for measurement of stable isotope tracer
TR:TREATMENT_SUMMARY             	enrichment and amino acid concentrations. "
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Plasma lysine and AAA concentrations and isotopic enrichment will be analyzed as
SP:SAMPLEPREP_SUMMARY            	previous described via a Thermo Fisher Q Exactive plus mass spectrometer coupled
SP:SAMPLEPREP_SUMMARY            	with a Dionex UltiMate 3000 Binary RSLC liquid chromatograph.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Dionex Ultimate 3000
CH:COLUMN_NAME                   	Unspecified
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Plus Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	MPE (molar percent excess )
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	MPE
MS_METABOLITE_DATA_START
Samples	ms6436-12	ms6436-13	ms6436-14	ms6436-15	ms6436-16	ms6436-17	ms6436-18	ms6436-19	ms6436-20	ms6436-21	ms6436-22	ms6437-12	ms6437-13	ms6437-14	ms6437-15	ms6437-16	ms6437-17	ms6437-18	ms6437-19	ms6437-20	ms6437-21	ms6437-22	ms6438-12	ms6438-13	ms6438-14	ms6438-15	ms6438-16	ms6438-17	ms6438-18	ms6438-19	ms6438-20	ms6438-21	ms6438-22	ms6439-12	ms6439-13	ms6439-14	ms6439-15	ms6439-16	ms6439-17	ms6439-18	ms6439-19	ms6439-20	ms6439-21	ms6439-22	ms6436-1	ms6436-2	ms6436-3	ms6436-4	ms6436-5	ms6436-6	ms6436-7	ms6436-8	ms6436-9	ms6436-10	ms6436-11	ms6437-1	ms6437-2	ms6437-3	ms6437-4	ms6437-5	ms6437-6	ms6437-7	ms6437-8	ms6437-9	ms6437-10	ms6437-11	ms6438-1	ms6438-2	ms6438-3	ms6438-4	ms6438-5	ms6438-6	ms6438-7	ms6438-8	ms6438-9	ms6438-10	ms6438-11	ms6439-1	ms6439-2	ms6439-3	ms6439-4	ms6439-5	ms6439-6	ms6439-7	ms6439-8	ms6439-9	ms6439-10	ms6439-11	ms6440-12	ms6440-13	ms6440-14	ms6440-15	ms6440-16	ms6440-17	ms6440-18	ms6440-19	ms6440-20	ms6440-21	ms6440-22	ms6441-12	ms6441-13	ms6441-14	ms6441-15	ms6441-16	ms6441-17	ms6441-18	ms6441-19	ms6441-20	ms6441-21	ms6441-22	ms6442-12	ms6442-13	ms6442-14	ms6442-15	ms6442-16	ms6442-17	ms6442-18	ms6442-19	ms6442-20	ms6442-21	ms6442-22	ms6443-12	ms6443-13	ms6443-14	ms6443-15	ms6443-16	ms6443-17	ms6443-18	ms6443-19	ms6443-20	ms6443-21	ms6443-22	ms6440-1	ms6440-2	ms6440-3	ms6440-4	ms6440-5	ms6440-6	ms6440-7	ms6440-8	ms6440-9	ms6440-10	ms6440-11	ms6441-1	ms6441-2	ms6441-3	ms6441-4	ms6441-5	ms6441-6	ms6441-7	ms6441-8	ms6441-9	ms6441-10	ms6441-11	ms6442-1	ms6442-2	ms6442-3	ms6442-4	ms6442-5	ms6442-6	ms6442-7	ms6442-8	ms6442-9	ms6442-10	ms6442-11	ms6443-1	ms6443-2	ms6443-3	ms6443-4	ms6443-5	ms6443-6	ms6443-7	ms6443-8	ms6443-9	ms6443-10	ms6443-11	ms6712-1	ms6712-2	ms6712-3	ms6712-4	ms6712-5	ms6712-6	ms6712-7	ms6712-8	ms6712-9	ms6712-10	ms6712-11	ms6712-12	ms6712-13	ms6712-14	ms6712-15	ms6712-16	ms6712-17	ms6712-18	ms6712-19	ms6712-20	ms6712-21	ms6712-22	ms6713-1	ms6713-2	ms6713-3	ms6713-4	ms6713-5	ms6713-6	ms6713-7	ms6713-8	ms6713-9	ms6713-10	ms6713-11	ms6713-12	ms6713-13	ms6713-14	ms6713-15	ms6713-16	ms6713-17	ms6713-18	ms6713-19	ms6713-20	ms6713-21	ms6713-22	ms6714-1	ms6714-2	ms6714-3	ms6714-4	ms6714-5	ms6714-6	ms6714-7	ms6714-8	ms6714-9	ms6714-10	ms6714-11	ms6714-12	ms6714-13	ms6714-14	ms6714-15	ms6714-16	ms6714-17	ms6714-18	ms6714-19	ms6714-20	ms6714-21	ms6714-22	ms6715-1	ms6715-2	ms6715-3	ms6715-4	ms6715-5	ms6715-6	ms6715-7	ms6715-8	ms6715-9	ms6715-10	ms6715-11	ms6715-12	ms6715-13	ms6715-14	ms6715-15	ms6715-16	ms6715-17	ms6715-18	ms6715-19	ms6715-20	ms6715-21	ms6715-22	ms6716-1	ms6716-2	ms6716-3	ms6716-4	ms6716-5	ms6716-6	ms6716-7	ms6716-8	ms6716-9	ms6716-10	ms6716-11	ms6716-12	ms6716-13	ms6716-14	ms6716-15	ms6716-16	ms6716-17	ms6716-18	ms6716-19	ms6716-20	ms6716-21	ms6716-22	ms6717-1	ms6717-2	ms6717-3	ms6717-4	ms6717-5	ms6717-6	ms6717-7	ms6717-8	ms6717-9	ms6717-10	ms6717-11	ms6717-12	ms6717-13	ms6717-14	ms6717-15	ms6717-16	ms6717-17	ms6717-18	ms6717-19	ms6717-20	ms6717-21	ms6717-22	ms6718-1	ms6718-2	ms6718-3	ms6718-4	ms6718-5	ms6718-6	ms6718-7	ms6718-8	ms6718-9	ms6718-10	ms6718-11	ms6718-12	ms6718-13	ms6718-14	ms6718-15	ms6718-16	ms6718-17	ms6718-18	ms6718-19	ms6718-20	ms6718-21	ms6718-22
Factors	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS	Time point:baseline | group:PCOS	Time point:-60 | group:PCOS	Time point:-45 | group:PCOS	Time point:-30 | group:PCOS	Time point:-15 | group:PCOS	Time point:60 | group:PCOS	Time point:120 | group:PCOS	Time point:135 | group:PCOS	Time point:150 | group:PCOS	Time point:165 | group:PCOS	Time point:180 | group:PCOS
15N-aminoadipic	0	1.419436871	1.651575973	1.516551432	1.925875442	2.440514346	2.954767841	2.924705598	3.565144116	3.159729595	3.58242166	0	1.854837007	2.365833622	2.602638805	2.404944742	3.284354622	3.457952457	4.061364168	4.264112908	3.565900565	4.418813066	0	1.62106986	1.682681187	1.580491348	1.677853414	2.002944831	2.489176274	2.289774813	2.575414703	2.629486099	2.588097667	0	1.677354557	1.978536259	1.762027607	1.864920703	2.013051859	2.006981192	2.445945529	2.459083259	2.666545034	2.997049923	0	1.728769225	2.076277822	1.915960337	2.167952546	2.344018149	2.852380612	2.872293949	3.052522996	3.320910561	3.269550983	0	1.656368735	2.101297986	2.572511625	2.620360634	2.807166125	3.156901614	3.080737812	3.424149742	3.456776507	2.625631711	0	1.42756818	1.595499785	1.585737045	1.237123027	1.200148622	1.896327499	2.303554981	2.518782498	2.508427964	3.133943146	0	1.199092502	1.356698771	1.661946823	1.615429884	1.835186669	1.975903537	2.226331475	2.340877619	2.556079065	2.066047986	0	1.826793466	1.6779617	1.900828102	2.220204306	2.507583133	3.454262416	3.014373974	3.008258862	3.371208076	3.411371284	0	2.022673263	1.758577852	1.875163169	2.027034795	2.604111317	2.932368585	3.719054819	3.313392624	3.299072069	3.514938027	0	2.12278341	2.491098632	2.649605842	2.533702314	3.55886236	4.034807633	4.462365271	4.968518762	4.441674422	4.765881548	0	1.319350407	2.2816102	2.735761438	3.165976484	2.689473194	2.951393075	3.29612396	3.528504393	3.69899286	4.188390362	0	1.22302872	1.661299899	2.015674999	1.675	2.478392084	2.692045325	2.75241358	2.715741901	3.265614126	2.91162279	0	2.171788535	2.207890692	2.616952687	2.520740174	3.228583253	3.736756839	4.698097767	5.43583748	4.111979675	4.515780521	0	2.603242491	2.114775383	2.445030007	0	3.355860012	3.818067354	4.307163651	4.563517542	4.846540819	4.171610859	0	1.759738936	2.013156077	2.085401384	2.428796282	2.803218411	2.570622704	2.5239703	1.187241453	2.77033411	2.879591563	0	1.492341812	1.440722425	1.578452897	1.694691359	2.785296293	2.810898101	2.880190071	3.139928885	2.857108605	3.303394238	0	-0.061107498	1.456240541	1.414816362	1.510678433	2.171632561	2.761606474	2.888794327	3.232406628	3.154015647	3.035959611	0	2.179527252	2.236272606	2.594463114	2.558413958	3.401529522	3.757911471	4.508671714	4.555062701	4.581573421	4.920226609	0	2.569214444	2.631498967	2.779078803	3.323902207	3.81193314	4.975812445	4.87010249	5.119194834	4.900039013	4.685050029	0	1.7952151	1.930303572	1.981577159	2.035936428	2.282866432	3.410007799	3.069358423	3.045024625	3.115359109	3.328321466	0	1.664948411	2.052504324	2.233783098	2.244190796	2.795932992	3.249376413	3.758556244	3.330850953	3.911318481	3.85404564	0	1.556765401	1.42878909	1.575309378	1.607729961	2.060857401	2.41636709	2.337460538	2.607804411	2.676200161	2.96353117	0	1.328232593	1.51542218	1.52997878	1.691298256	2.063919655	2.798316825	2.516513447	2.516314708	2.653106589	2.849190541	0	2.19210621	2.640397623	2.398682305	2.566539792	3.303378069	4.060852164	4.459493734	4.645068465	4.30821293	5.026542651	0	2.409539076	2.526720301	2.808342537	2.78888365	3.816782336	4.42139144	4.562549757	4.673426812	4.882844374	4.729838747	0	1.77776146	1.500844551	1.354377933	1.746810979	2.064964603	2.934208305	2.554879914	2.545528734	2.993669441	2.701444318	0	1.691293468	1.698712184	1.783340876		2.821711134	3.2826759	3.282308418	3.154184869	3.677790599	3.884881103	0	1.812721461	1.847983627	2.183334747	1.894004847	2.64989941	2.929068604	3.410885957	3.444247826	3.270906332	3.189832627	0	2.00104263	2.343247982	2.431084395	2.29789577	2.749122925	3.063773264	3.752742208	3.446128169	3.985933775	3.468398923
13C-aminoadipic	0	8.439428675	9.348360168	8.495687194	10.34508512	13.05603872	13.0644151	11.38108745	16.75076902	10.98401322	12.55609396	0	15.461869	17.78100951	18.14421406	15.96409044	18.97299739	16.37590734	19.57285373	19.41206566	17.2107602	19.80051132	0	7.996805808	8.466232407	7.757959285	8.268066261	9.819515762	9.286896079	9.619515176	9.831663103	9.95677832	10.47111603	0	7.081492671	7.349935825	8.68490396	7.568167968	7.804504947	8.532722716	9.250784999	9.036211923	8.684181695	8.06313244	0	12.02029917	12.52697042	10.35550182	12.51167386	11.71337241	13.20102706	13.96823118	15.42875504	14.2794118	13.63900409	0	15.87970088	16.67526895	16.66078925	15.64122307	14.22159903	15.04390028	11.78946308	14.85832427	16.13419152	9.394640889	0	9.916968228	9.485014527	8.389504251	4.264193018	4.018991183	9.521924955	10.79635077	12.1154193	10.70619056	10.95703426	0	7.266168793	7.463153861	7.24226486	8.994960516	7.24352844	8.319936643	7.802770664	9.345815868	10.12585071	7.872668425	0	9.773250683	8.843898195	7.911851222	8.9224448	11.38316375	11.35973183	11.54215689	11.96169288	11.24325448	11.81066893	0	13.00600004	12.1402721	10.62420099	12.30697945	11.52213364	11.56276462	14.59727261	13.73978521	11.43078412	13.85844466	0	11.33173147	10.41071262	10.56854067	11.22065499	10.88856885	13.10489917	14.22470444	14.1395262	14.93516758	12.68634519	0	11.91792325	12.19315435	10.62355011	9.842093076	12.89917172	13.83598865	12.91513013	12.9424747	9.109924465	7.986454396	0	7.906283302	8.935123436	9.385430274	8.828	10.30568111	12.83672518	10.82325284	13.27454075	12.48245573	11.90821086	0	12.56109784	11.38971839	13.97102736	13.6975812	15.18239004	12.53059766	14.76945489	18.07737292	12.39783682	12.43336435	0	13.7264744	14.49013024	15.59360558	364.6197489	17.59596226	18.37273175	21.5870295	21.83042001	23.48770435	19.09139148	0	10.53437359	11.3477045	9.369392151	11.09028128	12.8901957	13.42885149	11.62387675	12.508298	11.68503645	12.96287291	0	8.492755079	9.033000107	9.624484205	8.018849324	11.61088991	12.65579113	12.98436139	13.51619076	13.06329921	12.07141851	0	0	5.732104481	5.130080773	5.933587957	6.384615685	7.570719373	8.352588522	7.55007082	6.719245123	7.342915726	0	13.47036486	14.27566538	13.09667488	14.11616302	13.12371737	14.13656871	15.11846869	13.45026015	14.67983733	14.66512298	0	17.189365	15.40887094	14.93097801	15.73486063	14.35651864	15.50940693	15.55146094	16.11580476	15.52699248	16.4996068	0	10.29456174	11.89789321	10.81494189	11.50924683	10.65095752	13.00086962	12.56776227	11.81939765	13.23963146	14.8045027	0	9.868824801	9.832611003	10.27124803	10.94967756	13.50903187	12.15437669	14.29713399	11.64659	12.6823176	13.90565465	0	8.269857769	7.960881493	7.701564253	9.142063637	8.893619397	10.50275226	9.673255907	10.00332625	10.57953137	11.31526456	0	9.643747489	10.28954656	9.851247574	11.1484682	10.8641617	12.77828831	11.83846505	11.73014397	11.45932056	12.22807729	0	17.91185341	16.85384749	15.75862165	15.31990156	16.94738554	16.60397412	17.42241021	16.78292215	16.1585128	18.36870436	0	21.91527764	20.65307871	22.00816014	20.11323392	21.42547028	23.15410165	21.50314638	22.81439463	19.9778791	21.11845643	0	10.38184797	9.232902012	8.304360755	9.321299696	11.79518891	12.46959737	12.49994087	12.08936881	12.85298749	10.55434531	0	12.99632407	11.85143384	12.54871467		17.31754895	17.11497684	15.59300987	16.0158713	16.67567138	14.35993762	0	11.37050415	10.55339658	12.75171376	10.35940583	13.0682553	12.06466158	12.99465322	12.99599625	11.83906367	11.23738275	0	11.501612	9.827469466	10.94253777	10.52570945	12.12073318	12.80991882	13.35871826	13.63930481	13.11946807	12.54769192
15N-Lysine	0	7.362374948	7.592793212	6.89623434	7.992661018	7.683198267	7.937965965	8.078362384	8.136317094	7.912928234	7.835071184	0	7.63473941	7.136280679	8.121458566	8.00362972	9.325491894	8.619465126	9.307610235	9.447260452	9.557171274	9.103978951	0	7.369027205	6.878183228	7.025942249	6.175856324	8.132781156	7.978622136	8.685113979	7.901630822	8.147110946	8.25404362	0	5.72595797	5.74508738	6.56833485	6.227725534	7.838193519	7.502596468	7.341887555	7.581063691	7.994690412	7.873503865	0	7.377554375	7.56331685	7.525262023	7.749096056	8.087884497	8.627785279	8.792068838	9.925093035	8.641197488	8.599294626	0	7.320631986	8.110503668	8.122123143	8.633882973	8.884625171	8.21146413	9.149831425	8.612784913	9.25719442	10.04100895	0	7.446349169	6.728108304	7.636097022	7.001705654	7.342170501	8.13438194	8.74774809	7.642190749	9.482560874	8.290938389	0	6.738561073	6.525564008	6.371279559	7.565881617	7.306598101	7.741176419	7.924634429	9.299643982	8.11447592	7.992674688	0	6.657722911	4.568780747	5.95508191	6.3417871	7.095972472	6.743704054	7.542313714	8.176528904	7.01131565	7.312646858	0	4.371524496	5.422867818	5.013026379	4.861625965	6.061537053	6.007554735	6.367180598	5.86241897	5.895116406	5.633323348	0	6.482216011	6.115414276	6.384763345	6.112055589	6.55094369	7.577479465	8.823230493	7.817699155	8.059480465	8.116718961	0	6.623563677	6.774902076	6.046429789	6.084182455	7.319924294	7.143344364	7.863237227	7.757819506	5.862508569	5.050685314	0	5.190149779	7.313995465	6.429875208	6.138963239	8.021404283	8.197078789	8.696746991	8.109530373	8.447934589	7.226596563	0	7.673184668	8.136942604	8.177642455	8.419011631	7.180441192	8.231485042	9.574841558	7.937078987	8.333482148	9.088528714	0	6.825831284	6.855608005	9.306214544	477.2303044	8.94638512	10.24686894	9.390525792	8.727828124	9.178078093	9.684290773	0	7.543179981	6.917224404	6.584463479	6.345369142	8.321570954	8.949000224	9.143433756	8.240086211	7.785714674	8.87354094	0	5.731664049	6.727354299	6.624356173	5.933729262	6.287640178	7.835197649	7.296592273	7.851026924	8.248676289	8.021760544	0	0.624167702	5.201267524	3.884580023	5.552752972	5.463535115	4.300809186	4.350014211	5.796943663	5.922286468	-6.351436654	0	7.243051069	7.640244825	6.263870427	7.682123693	7.345353356	8.63467635	7.664394665	7.540815362	8.183971802	7.567231036	0	6.743516672	7.941262295	7.563559928	8.089799219	7.899023585	8.307012739	8.591015379	9.024563468	8.789796812	8.831657749	0	5.848254925	6.840675649	6.556959755	6.526657552	6.931712775	6.674619737	8.097586256	7.383318912	8.498021654	8.221773498	0	7.506220009	5.749040349	6.137910943	7.04883267	6.911209643	7.701476056	8.628377818	8.01269234	7.801956637	7.350053888	0	6.253804514	5.928585495	5.531512557	6.3874971	7.776946743	7.542968169	7.543665309	6.532994181	6.973544749	7.039029431	0	6.219512407	6.12530219	6.245166833	7.078289308	7.312043313	6.744052248	7.890256592	8.038511437	7.952859962	7.04965785	0	7.324471455	7.557302395	8.479085714	8.356431556	8.028333679	8.194083275	9.188144842	8.272782478	9.240812303	9.229507542	0	7.527333938	8.103767447	8.534231663	8.373318251	8.211297217	8.67014962	9.748695812	8.87935001	9.274813389	9.096252623	0	6.899590169	8.183823569	8.525116915	8.075934763	7.354932971	8.053042353	7.798625987	8.276567854	8.318398584	7.924532766	0	6.807780466	7.112156451	7.085430489		8.490878523	8.622697138	8.603222408	8.489884401	7.876505914	7.732054991	0	6.418580225	6.007648684	6.045456808	6.391711151	7.472082236	7.491513466	6.820021795	7.14608026	6.342498491	7.542698817	0	6.732327034	6.459310344	6.44588974	6.321202492	7.186737967	8.376912297	7.820687855	8.248757571	7.380657515	7.73960769
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
15N-aminoadipic
13C-aminoadipic
15N-Lysine
METABOLITES_END
#END