#METABOLOMICS WORKBENCH hormel101_20180717_161349_mwtab.txt DATATRACK_ID:1457 STUDY_ID:ST001020 ANALYSIS_ID:AN001668 PROJECT_ID:PR000683 VERSION 1 CREATED_ON July 18, 2018, 10:03 am #PROJECT PR:PROJECT_TITLE Mayo Pilot and Feasibility: Characterization of metabolomics profile changes PR:PROJECT_TITLE during development of post-traumatic epilepsy PR:PROJECT_SUMMARY According to the report from the Centers for Disease Control and Prevention in PR:PROJECT_SUMMARY 2014, traumatic brain injury (TBI) accounts for 30% of all injury-related deaths PR:PROJECT_SUMMARY in the U.S. Developing epilepsy after severe head injury is as high as 40%-50% PR:PROJECT_SUMMARY in some settings. Importantly, many TBI victims develop epilepsy months or years PR:PROJECT_SUMMARY following the initial injury. However, it has not been fully identified how to PR:PROJECT_SUMMARY predict who will develop epilepsy and/or the underlying mechanism of post PR:PROJECT_SUMMARY traumatic epilesy (PTE) development. The main goal of this proposal is to PR:PROJECT_SUMMARY identify the metabolomics biomarker of TBI-induced epilepsy and to investigate PR:PROJECT_SUMMARY the underlying mechanism of the transition from TBI to PTE. To do so, we will PR:PROJECT_SUMMARY develop PTE using two TBI animal models: ferrous chloride injection model and PR:PROJECT_SUMMARY cortical undercut model. Once the brain damage is made, electroencephalogram PR:PROJECT_SUMMARY (EEG) and video monitoring will be performed to determine the onset point of PR:PROJECT_SUMMARY epilepsy. Then, metabolomics profile changes will be analyzed and compared PR:PROJECT_SUMMARY before and after PTE development. PR:INSTITUTE Mayo Clinic PR:LAST_NAME Su-youne PR:FIRST_NAME Chang PR:ADDRESS 200 1st Street SW Rochester, MN 55905, USA PR:EMAIL Chang.SuYoune@mayo.edu PR:PHONE 1-507-293-0511 #STUDY ST:STUDY_TITLE Characterization of metabolomics profile changes during development of ST:STUDY_TITLE post-traumatic epilepsy in Rat Plasma (part-I) ST:STUDY_SUMMARY Characterize the metabolomics profile changes during progression of the ST:STUDY_SUMMARY transition from traumatic brain injury (TBI) to post-traumatic epilepsy (PTE). ST:STUDY_SUMMARY To do so, three experiments will be performed. PTE animal model will be ST:STUDY_SUMMARY developed using ferrous chloride injections. Metabolomics profile changes will ST:STUDY_SUMMARY be obtained before TBI, after TBI, and after PTE development These temporal ST:STUDY_SUMMARY changes in metabolomics profile during the course of PTE development will be ST:STUDY_SUMMARY collected. We will also collect cerebrospinal fluid (CSF) at each time point. In ST:STUDY_SUMMARY addition, we will collect the brain tissue from the center of injury, around the ST:STUDY_SUMMARY injury, and from the non-injured area for mass spectrometry. In this study, the ST:STUDY_SUMMARY rat plasma at each time point is analyzed. ST:INSTITUTE Mayo Clinic ST:LAST_NAME Su-youne ST:FIRST_NAME Chang ST:ADDRESS 200 1st Street SW Rochester, MN 55905, USA ST:EMAIL Chang.SuYoune@mayo.edu ST:PHONE 1-507-293-0511 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10116 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS ms6756-1 nC18-22jul17-001 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-2 nC18-22jul17-002 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-3 nC18-22jul17-003 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-4 nC18-22jul17-004 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-5 nC18-22jul17-005 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-6 nC18-22jul17-006 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-7 nC18-22jul17-007 groups:C | time:C2 Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-8 nC18-22jul17-008 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-9 nC18-22jul17-009 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-10 nC18-22jul17-010 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-11 nC18-22jul17-011 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-12 nC18-22jul17-012 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-13 nC18-22jul17-013 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-14 nC18-22jul17-014 groups:C | time:C3 Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-15 nC18-22jul17-015 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-16 nC18-22jul17-016 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-17 nC18-22jul17-017 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-18 nC18-22jul17-018 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-19 nC18-22jul17-019 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-20 nC18-22jul17-020 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-21 nC18-22jul17-021 groups:C | time:C4 Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-22 nC18-22jul17-022 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-23 nC18-22jul17-023 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-24 nC18-22jul17-024 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-25 nC18-22jul17-025 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-26 nC18-22jul17-026 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-27 nC18-22jul17-027 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=NC18 SUBJECT_SAMPLE_FACTORS ms6756-1 pC18-20jul17-001 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-2 pC18-20jul17-002 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-3 pC18-20jul17-003 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-4 pC18-20jul17-004 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-5 pC18-20jul17-005 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-6 pC18-20jul17-006 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-7 pC18-20jul17-007 groups:C | time:C2 Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-8 pC18-20jul17-008 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-9 pC18-20jul17-009 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-10 pC18-20jul17-010 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-11 pC18-20jul17-011 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-12 pC18-20jul17-012 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-13 pC18-20jul17-013 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-14 pC18-20jul17-014 groups:C | time:C3 Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-15 pC18-20jul17-015 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-16 pC18-20jul17-016 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-17 pC18-20jul17-017 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-18 pC18-20jul17-018 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-19 pC18-20jul17-019 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-20 pC18-20jul17-020 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-21 pC18-20jul17-021 groups:C | time:C4 Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-22 pC18-20jul17-022 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-23 pC18-20jul17-023 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-24 pC18-20jul17-024 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-25 pC18-20jul17-025 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-26 pC18-20jul17-026 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-27 pC18-20jul17-027 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=PC18 SUBJECT_SAMPLE_FACTORS ms6756-1 nhilic-28jul17-001 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-2 nhilic-28jul17-002 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-3 nhilic-28jul17-003 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-4 nhilic-28jul17-004 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-5 nhilic-28jul17-005 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-6 nhilic-28jul17-006 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-7 nhilic-28jul17-007 groups:C | time:C2 Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-8 nhilic-28jul17-008 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-9 nhilic-28jul17-009 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-10 nhilic-28jul17-010 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-11 nhilic-28jul17-011 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-12 nhilic-28jul17-012 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-13 nhilic-28jul17-013 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-14 nhilic-28jul17-014 groups:C | time:C3 Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-15 nhilic-28jul17-015 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-16 nhilic-28jul17-016 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-17 nhilic-28jul17-017 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-18 nhilic-28jul17-018 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-19 nhilic-28jul17-019 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-20 nhilic-28jul17-020 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-21 nhilic-28jul17-021 groups:C | time:C4 Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-22 nhilic-28jul17-022 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-23 nhilic-28jul17-023 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-24 nhilic-28jul17-024 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-25 nhilic-28jul17-025 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-26 nhilic-28jul17-026 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-27 nhilic-28jul17-027 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=nhilic SUBJECT_SAMPLE_FACTORS ms6756-1 philic-17jul17-001 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-2 philic-17jul17-002 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-3 philic-17jul17-003 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-4 philic-17jul17-004 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-5 philic-17jul17-005 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-6 philic-17jul17-006 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-7 philic-17jul17-007 groups:C | time:C2 Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-8 philic-17jul17-008 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-9 philic-17jul17-009 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-10 philic-17jul17-010 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-11 philic-17jul17-011 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-12 philic-17jul17-012 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-13 philic-17jul17-013 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-14 philic-17jul17-014 groups:C | time:C3 Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-15 philic-17jul17-015 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-16 philic-17jul17-016 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-17 philic-17jul17-017 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-18 philic-17jul17-018 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-19 philic-17jul17-019 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-20 philic-17jul17-020 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-21 philic-17jul17-021 groups:C | time:C4 Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-22 philic-17jul17-022 groups:S | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-23 philic-17jul17-023 groups:F | time:Pre Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-24 philic-17jul17-024 groups:S | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-25 philic-17jul17-025 groups:F | time:2W Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-26 philic-17jul17-026 groups:S | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=philic SUBJECT_SAMPLE_FACTORS ms6756-27 philic-17jul17-027 groups:F | time:M1(4W) Gender=male; Sample type=plasma; Species=rat; chromatography=philic #COLLECTION CO:COLLECTION_SUMMARY Rats will either be controls, injected with saline, or injected with ferrous CO:COLLECTION_SUMMARY chloride to influence PTE. Study Groupings: C=control, S=Saline treated, CO:COLLECTION_SUMMARY F=ferrous chloride treated Time: pre=day0, 2W=2 weeks, M1(4W)=1 month CO:COLLECTION_SUMMARY Experimental Flow Day0: baseline pre TBI. Blood and CSF collected Day1: Surgery CO:COLLECTION_SUMMARY for TBI. Injections of Ferrous Cloride or Saline Day2: CSF collected Weeks1-3: CO:COLLECTION_SUMMARY montoring to determine PTE starting point. Blood and CSF collected 1 Month: CO:COLLECTION_SUMMARY montoring of PTE. Blood and CSF collected 2 Month: Animal is euthanized and CO:COLLECTION_SUMMARY blood, CSF, and tissue harvested CO:SAMPLE_TYPE Blood (plasma) #TREATMENT TR:TREATMENT_SUMMARY "Rats will either be controls, injected with saline, or injected with ferrous TR:TREATMENT_SUMMARY chloride to influence PTE. Trauma-Induced Epilepsy Model: Ferrous chloride TR:TREATMENT_SUMMARY injection model: Ferrous chloride solution (5 μl of 100 mM with saline) will be TR:TREATMENT_SUMMARY injected at a rate of 0.5 μl/min through a Hamilton micro-syringe controlled by TR:TREATMENT_SUMMARY a micro-pump (UMP3, WPI, FL). Once the ferrous chloride solution injection is TR:TREATMENT_SUMMARY completed, the syringe will remain in position for 5 minutes, and then it will TR:TREATMENT_SUMMARY be removed slowly. The burr holes will be closed with light-curing dental TR:TREATMENT_SUMMARY cement. The dose of ferrous chloride injection was determined from prior TR:TREATMENT_SUMMARY published reports from mouse, rat, and cat. They all used 100 mM ferrous TR:TREATMENT_SUMMARY chloride aqueous solution and volumes were various: 1 μl for mouse,14 5 μl for TR:TREATMENT_SUMMARY rat (200-300 g),15 and 10 μl for cat (2-4 Kg).15 Video monitoring: The use of TR:TREATMENT_SUMMARY 24 x 7 video monitoring and review means that we do not have to rely on the rats TR:TREATMENT_SUMMARY having seizures during daily rounding or at some other time when a human happens TR:TREATMENT_SUMMARY to be present in the home cage. Normally, the video will be watched in time TR:TREATMENT_SUMMARY lapse, fast-forward mode to scan for potential seizures. The reviewer can then TR:TREATMENT_SUMMARY stop the video, rewind and watch the behavioral episode in real-time or slow TR:TREATMENT_SUMMARY motion to determine whether a seizure actually occurred. Behavioral seizures TR:TREATMENT_SUMMARY will be identified by any combination or sequence of the following: loss of TR:TREATMENT_SUMMARY postural control (opisthotonus), tonic flexion or extension of limbs or TR:TREATMENT_SUMMARY head/neck, and clonic movements of limbs or head/neck. Often, behavioral TR:TREATMENT_SUMMARY seizures in rats may be accompanied by drooling, urination and facial twitches, TR:TREATMENT_SUMMARY although these may not always be observable on video. In addition, seizures will TR:TREATMENT_SUMMARY likely be followed by a postictal phase, which may include a period of running, TR:TREATMENT_SUMMARY jumping and general agitation. Video monitoring cannot detect subclinical or TR:TREATMENT_SUMMARY electrographic seizures (i.e., seizures without a behavioral component). Video TR:TREATMENT_SUMMARY will be reviewed in this way for each rat in order to establish that a cortical TR:TREATMENT_SUMMARY injured rat does indeed have epilepsy, to establish the “typical seizure” TR:TREATMENT_SUMMARY pattern in each rat, and to help establish a seizure frequency baseline, TR:TREATMENT_SUMMARY although it is understood that video monitoring alone might occasionally miss a TR:TREATMENT_SUMMARY seizure. EEG monitoring: To prevent imaging distortion and ferromagnetic TR:TREATMENT_SUMMARY interference, graphite carbon electrodes will be fabricated and/or purchased. A TR:TREATMENT_SUMMARY total of five electrodes will be implanted for EEG monitoring on the skull. EEG TR:TREATMENT_SUMMARY will be monitored with the Open EPhys System.18 While EEG recording, EEG TR:TREATMENT_SUMMARY electrodes will be connected to wires attached to the ceiling of a cage. In TR:TREATMENT_SUMMARY trauma-induced epilepsy rats, spontaneous neural activity will be recorded using TR:TREATMENT_SUMMARY a wide bandwidth (0-9 kHz) recording system. Post-analysis will be used to TR:TREATMENT_SUMMARY identify epilepsy signals." #SAMPLEPREP SP:SAMPLEPREP_SUMMARY large scale profiling using rat plasma #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent 1290 Infinity CH:COLUMN_NAME Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6550 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_RESULTS_FILE ST001020_AN001668_Results.txt UNITS:intensity #END