#METABOLOMICS WORKBENCH KunZuo_20190410_234734_mwtab.txt DATATRACK_ID:1700 STUDY_ID:ST001168 ANALYSIS_ID:AN001932 PROJECT_ID:PR000780 VERSION 1 CREATED_ON April 10, 2019, 11:57 pm #PROJECT PR:PROJECT_TITLE Alterations in fecal metabolic patterns are associated with atrial fibrillation PR:PROJECT_SUMMARY Little evidence has been reported in characterizing the fecal alterations in PR:PROJECT_SUMMARY metabolic patterns in atrial fibrillation (AF). We include the result of the PR:PROJECT_SUMMARY global alterations that occur in the intestinal microbiota in a cohort of AF PR:PROJECT_SUMMARY patients and matched controls based on a strategy of metabolomic analyses. Our PR:PROJECT_SUMMARY findings characterize the disordered microbial metabolite profiles in AF. PR:INSTITUTE Beijing Chaoyang Hospital PR:LAST_NAME Zuo PR:FIRST_NAME Kun PR:ADDRESS 8th Gongtinanlu Rd,, Chaoyang District, Beijing, Beijing, 10020, China PR:EMAIL zuokun699@163.com PR:PHONE 10-86-15210511744 #STUDY ST:STUDY_TITLE Alterations in fecal metabolic patterns are associated with atrial fibrillation ST:STUDY_SUMMARY Little evidence has been reported in characterizing the fecal alterations in ST:STUDY_SUMMARY metabolic patterns in atrial fibrillation (AF). We include the result of the ST:STUDY_SUMMARY global alterations that occur in the intestinal microbiota in a cohort of AF ST:STUDY_SUMMARY patients and matched controls based on a strategy of metabolomic analyses. Our ST:STUDY_SUMMARY findings characterize the disordered microbial metabolite profiles in AF. ST:INSTITUTE Beijing Chaoyang Hospital ST:LAST_NAME Kun ST:FIRST_NAME Zuo ST:ADDRESS 8th Gongtinanlu Rd, Chaoyang District, Beijing, China, 100020 ST:EMAIL zuokun699@163.com ST:PHONE 86-10-15210511744 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - af9 Group:A SUBJECT_SAMPLE_FACTORS - af10 Group:A SUBJECT_SAMPLE_FACTORS - af11 Group:A SUBJECT_SAMPLE_FACTORS - af12 Group:A SUBJECT_SAMPLE_FACTORS - af13 Group:A SUBJECT_SAMPLE_FACTORS - af14 Group:A SUBJECT_SAMPLE_FACTORS - af15 Group:A SUBJECT_SAMPLE_FACTORS - af16 Group:A SUBJECT_SAMPLE_FACTORS - af17 Group:A SUBJECT_SAMPLE_FACTORS - af18 Group:A SUBJECT_SAMPLE_FACTORS - af19 Group:A SUBJECT_SAMPLE_FACTORS - af20 Group:A SUBJECT_SAMPLE_FACTORS - af21 Group:A SUBJECT_SAMPLE_FACTORS - af22 Group:A SUBJECT_SAMPLE_FACTORS - af23 Group:A SUBJECT_SAMPLE_FACTORS - af24 Group:A SUBJECT_SAMPLE_FACTORS - af25 Group:A SUBJECT_SAMPLE_FACTORS - af26 Group:A SUBJECT_SAMPLE_FACTORS - af27 Group:A SUBJECT_SAMPLE_FACTORS - af28 Group:A SUBJECT_SAMPLE_FACTORS - af29 Group:A SUBJECT_SAMPLE_FACTORS - af30 Group:A SUBJECT_SAMPLE_FACTORS - af31 Group:A SUBJECT_SAMPLE_FACTORS - af32 Group:A SUBJECT_SAMPLE_FACTORS - af33 Group:A SUBJECT_SAMPLE_FACTORS - af34 Group:A SUBJECT_SAMPLE_FACTORS - af35 Group:A SUBJECT_SAMPLE_FACTORS - af36 Group:A SUBJECT_SAMPLE_FACTORS - af37 Group:A SUBJECT_SAMPLE_FACTORS - af38 Group:A SUBJECT_SAMPLE_FACTORS - af39 Group:A SUBJECT_SAMPLE_FACTORS - af40 Group:A SUBJECT_SAMPLE_FACTORS - af41 Group:A SUBJECT_SAMPLE_FACTORS - af42 Group:A SUBJECT_SAMPLE_FACTORS - af43 Group:A SUBJECT_SAMPLE_FACTORS - af44 Group:A SUBJECT_SAMPLE_FACTORS - af45 Group:A SUBJECT_SAMPLE_FACTORS - af46 Group:A SUBJECT_SAMPLE_FACTORS - af47 Group:A SUBJECT_SAMPLE_FACTORS - af48 Group:A SUBJECT_SAMPLE_FACTORS - af49 Group:A SUBJECT_SAMPLE_FACTORS - af50 Group:A SUBJECT_SAMPLE_FACTORS - FA6 Group:C SUBJECT_SAMPLE_FACTORS - FA7 Group:C SUBJECT_SAMPLE_FACTORS - FA9 Group:C SUBJECT_SAMPLE_FACTORS - FA13 Group:C SUBJECT_SAMPLE_FACTORS - FA4 Group:C SUBJECT_SAMPLE_FACTORS - FA2 Group:C SUBJECT_SAMPLE_FACTORS - FA12 Group:C SUBJECT_SAMPLE_FACTORS - FA3 Group:C SUBJECT_SAMPLE_FACTORS - FC10 Group:C SUBJECT_SAMPLE_FACTORS - FC12 Group:C SUBJECT_SAMPLE_FACTORS - FC2 Group:C SUBJECT_SAMPLE_FACTORS - FC17 Group:C SUBJECT_SAMPLE_FACTORS - FC29 Group:C SUBJECT_SAMPLE_FACTORS - FC14 Group:C SUBJECT_SAMPLE_FACTORS - FC16 Group:C SUBJECT_SAMPLE_FACTORS - FC13 Group:C SUBJECT_SAMPLE_FACTORS - FC9 Group:C #COLLECTION CO:COLLECTION_SUMMARY Fecal samples were collected from each participant, immediately frozen at −20 CO:COLLECTION_SUMMARY °C, transported on ice to the laboratory and then stored at −80 °C. CO:SAMPLE_TYPE Feces #TREATMENT TR:TREATMENT_SUMMARY To explore how the host metabolic pattern alterations were impacted by the gut TR:TREATMENT_SUMMARY microbiota dysbiosis in AF patients, serum samples were collected and analyzed TR:TREATMENT_SUMMARY by high-throughput liquid chromatography-mass spectrometry (LC/MS). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY 50 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation SP:SAMPLEPREP_SUMMARY for extraction. The protein was precipitated with 800 μL of methanol and 10 μL SP:SAMPLEPREP_SUMMARY of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The SP:SAMPLEPREP_SUMMARY samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min SP:SAMPLEPREP_SUMMARY at 4 °C. 200 μL of the supernatant was transferred into a vial for further SP:SAMPLEPREP_SUMMARY analysis. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase MS:INSTRUMENT_NAME Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap CH:COLUMN_NAME Thermo Hypergod C18 (100 × 4.6 mm 3 µm) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS All metabolomic data were prepared for feature extraction and preprocessed with MS:MS_COMMENTS Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB, MS:MS_COMMENTS Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular MS:MS_COMMENTS mass, ppm and ms/ms value of these compounds was used to identify the MS:MS_COMMENTS metabolites related to the featured peak in the Metlin database MS:MS_COMMENTS (http://metlin.scripps.edu). The score value indicated the matching rate was MS:MS_COMMENTS calculated by Compound Discoverer 2.0 software (Thermo) with max of 100. MS:MS_RESULTS_FILE ST001168_AN001932_Results.txt UNITS:feature area Has m/z:Neutral masses Has RT:Yes RT units:Minutes #END