#METABOLOMICS WORKBENCH Codreags00_20200327_075634 DATATRACK_ID:1959 STUDY_ID:ST001333 ANALYSIS_ID:AN002222 PROJECT_ID:PR000910 VERSION 1 CREATED_ON March 30, 2020, 10:57 am #PROJECT PR:PROJECT_TITLE Huntington’s Disease Genotype Suppresses Global Manganese-Responsive Processes PR:PROJECT_TYPE Untargeted Metabolomics analysis PR:PROJECT_SUMMARY Global untargeted metabolomics studies performed in the striatum tissue, the PR:PROJECT_SUMMARY brain region most sensitive to neurodegeneration in Huntington’s Disease, to PR:PROJECT_SUMMARY investigate global Mn-dependent and Mn-responsive biology following various Mn PR:PROJECT_SUMMARY exposures in a mouse model of HD. PR:INSTITUTE Vanderbilt University PR:DEPARTMENT Chemistry PR:LABORATORY Center for Innovative Technology PR:LAST_NAME Codreanu PR:FIRST_NAME Simona PR:ADDRESS 1234 Stevenson Center Lane PR:EMAIL simona.codreanu@vanderbilt.edu PR:PHONE 6158758422 #STUDY ST:STUDY_TITLE Huntington’s Disease Genotype Suppresses Global Manganese-Responsive Processes ST:STUDY_TYPE Untargeted metabolomics analysis ST:STUDY_SUMMARY Global untargeted metabolomics studies performed in the striatum tissue, the ST:STUDY_SUMMARY brain region most sensitive to neurodegeneration in Huntington’s Disease, to ST:STUDY_SUMMARY investigate global Mn-dependent and Mn-responsive biology following various Mn ST:STUDY_SUMMARY exposures in a mouse model of HD. ST:INSTITUTE Vanderbilt University ST:DEPARTMENT Chemistry ST:LABORATORY Center for Innovative Technology ST:LAST_NAME Codreanu ST:FIRST_NAME Simona ST:ADDRESS 1234 Stevenson Center Lane ST:EMAIL simona.codreanu@vanderbilt.edu ST:PHONE 6158758422 #SUBJECT SU:SUBJECT_TYPE Other SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN YAC128 and wildtype (WT) SU:AGE_OR_AGE_RANGE 12 week and 32 week SU:GENDER Male and female #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS 1796 41_1796_C_M_128_0 Sex:M | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP_41_1796_C_M_128_0 SUBJECT_SAMPLE_FACTORS 1807 89_1807_C_M_WT_5 Sex:M | Genotype:WT | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__89_1807_C_M_WT_5 SUBJECT_SAMPLE_FACTORS 1808 23_1808_C_M_WT_15 Sex:M | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__23_1808_C_M_WT_15 SUBJECT_SAMPLE_FACTORS 1809 26_1809_C_M_128_15 Sex:M | Genotype:128 | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__26_1809_C_M_128_15 SUBJECT_SAMPLE_FACTORS 1811 30_1811_C_M_128_50 Sex:M | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__30_1811_C_M_128_50 SUBJECT_SAMPLE_FACTORS 1813 95_1813_C_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__95_1813_C_M_WT_50 SUBJECT_SAMPLE_FACTORS 1814 113_1814_C_M_WT_5 Sex:M | Genotype:WT | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__113_1814_C_M_WT_5 SUBJECT_SAMPLE_FACTORS 1815 77_1815_C_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__77_1815_C_M_WT_0 SUBJECT_SAMPLE_FACTORS 1816 38_1816_C_M_WT_15 Sex:M | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__38_1816_C_M_WT_15 SUBJECT_SAMPLE_FACTORS 1817 91_1817_C_M_WT_15 Sex:M | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__91_1817_C_M_WT_15 SUBJECT_SAMPLE_FACTORS 1818 110_1818_C_M_128_5 Sex:M | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__110_1818_C_M_128_5 SUBJECT_SAMPLE_FACTORS 1821 131_1821_C_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__131_1821_C_M_WT_0 SUBJECT_SAMPLE_FACTORS 1822 76_1822_C_M_128_0 Sex:M | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__76_1822_C_M_128_0 SUBJECT_SAMPLE_FACTORS 1823 128_1823_C_M_WT_5 Sex:M | Genotype:WT | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__128_1823_C_M_WT_5 SUBJECT_SAMPLE_FACTORS 1824 129_1824_C_M_WT_15 Sex:M | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__129_1824_C_M_WT_15 SUBJECT_SAMPLE_FACTORS 1825 84_1825_C_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__84_1825_C_M_WT_50 SUBJECT_SAMPLE_FACTORS 1827 133_1827_C_F_WT_5 Sex:F | Genotype:WT | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__133_1827_C_F_WT_5 SUBJECT_SAMPLE_FACTORS 1828 79_1828_C_F_WT_15 Sex:F | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__79_1828_C_F_WT_15 SUBJECT_SAMPLE_FACTORS 1830 66_1830_C_M_128_15 Sex:M | Genotype:128 | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__66_1830_C_M_128_15 SUBJECT_SAMPLE_FACTORS 1831 82_1831_C_M_128_50 Sex:M | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__82_1831_C_M_128_50 SUBJECT_SAMPLE_FACTORS 1832 107_1832_C_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__107_1832_C_M_WT_0 SUBJECT_SAMPLE_FACTORS 1833 100_1833_C_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__100_1833_C_F_128_0 SUBJECT_SAMPLE_FACTORS 1834 69_1834_C_F_WT_50 Sex:F | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__69_1834_C_F_WT_50 SUBJECT_SAMPLE_FACTORS 1837 61_1837_C_M_128_0 Sex:M | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__61_1837_C_M_128_0 SUBJECT_SAMPLE_FACTORS 1838 5_1838_C_M_WT_15 Sex:M | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__5_1838_C_M_WT_15 SUBJECT_SAMPLE_FACTORS 1839 130_1839_C_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__130_1839_C_M_WT_50 SUBJECT_SAMPLE_FACTORS 1840 21_1840_A_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__21_1840_A_M_WT_50 SUBJECT_SAMPLE_FACTORS 1841 1_1841_C_F_128_5 Sex:F | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__1_1841_C_F_128_5 SUBJECT_SAMPLE_FACTORS 1842 68_1842_C_F_WT_15 Sex:F | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__68_1842_C_F_WT_15 SUBJECT_SAMPLE_FACTORS 1843 127_1843_C_F_WT_5 Sex:F | Genotype:WT | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__127_1843_C_F_WT_5 SUBJECT_SAMPLE_FACTORS 1843b 124_1843b_C_F_128_15 Sex:F | Genotype:128 | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__124_1843b_C_F_128_15 SUBJECT_SAMPLE_FACTORS 1845 56_1845_C_M_128_5 Sex:M | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__56_1845_C_M_128_5 SUBJECT_SAMPLE_FACTORS 1847 111_1847_C_F_128_50 Sex:F | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__111_1847_C_F_128_50 SUBJECT_SAMPLE_FACTORS 1848 22_1848_C_F_WT_15 Sex:F | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__22_1848_C_F_WT_15 SUBJECT_SAMPLE_FACTORS 1849 55_1849_C_F_WT_0 Sex:F | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__55_1849_C_F_WT_0 SUBJECT_SAMPLE_FACTORS 1850 50_1850_C_F_WT_50 Sex:F | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__50_1850_C_F_WT_50 SUBJECT_SAMPLE_FACTORS 1852 32_1852_C_M_WT_5 Sex:M | Genotype:WT | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__32_1852_C_M_WT_5 SUBJECT_SAMPLE_FACTORS 1855 94_1855_C_F_WT_5 Sex:F | Genotype:WT | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__94_1855_C_F_WT_5 SUBJECT_SAMPLE_FACTORS 1856 28_1856_C_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__28_1856_C_F_128_0 SUBJECT_SAMPLE_FACTORS 1857 135_1857_C_F_128_5 Sex:F | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__135_1857_C_F_128_5 SUBJECT_SAMPLE_FACTORS 1858 64_1858_C_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__64_1858_C_M_WT_50 SUBJECT_SAMPLE_FACTORS 1860 102_1860_C_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__102_1860_C_M_WT_0 SUBJECT_SAMPLE_FACTORS 1861 112_1861_C_M_128_50 Sex:M | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__112_1861_C_M_128_50 SUBJECT_SAMPLE_FACTORS 1862 125_1862_C_M_WT_5 Sex:M | Genotype:WT | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__125_1862_C_M_WT_5 SUBJECT_SAMPLE_FACTORS 1864 73_1864_C_F_128_15 Sex:F | Genotype:128 | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__73_1864_C_F_128_15 SUBJECT_SAMPLE_FACTORS 1865 86_1865_C_F_WT_15 Sex:F | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__86_1865_C_F_WT_15 SUBJECT_SAMPLE_FACTORS 1866 78_1866_C_F_WT_50 Sex:F | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__78_1866_C_F_WT_50 SUBJECT_SAMPLE_FACTORS 1867 123_1867_C_F_128_50 Sex:F | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__123_1867_C_F_128_50 SUBJECT_SAMPLE_FACTORS 1869 80_1869_C_F_WT_0 Sex:F | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__80_1869_C_F_WT_0 SUBJECT_SAMPLE_FACTORS 1871 54_1871_C_M_128_5 Sex:M | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__54_1871_C_M_128_5 SUBJECT_SAMPLE_FACTORS 1872 59_1872_C_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__59_1872_C_M_WT_50 SUBJECT_SAMPLE_FACTORS 1874 104_1874_C_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__104_1874_C_F_128_0 SUBJECT_SAMPLE_FACTORS 1875 87_1875_C_F_WT_5 Sex:F | Genotype:WT | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__87_1875_C_F_WT_5 SUBJECT_SAMPLE_FACTORS 1876 37_1876_C_F_WT_15 Sex:F | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__37_1876_C_F_WT_15 SUBJECT_SAMPLE_FACTORS 1877 85_1877_C_F_128_5 Sex:F | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__85_1877_C_F_128_5 SUBJECT_SAMPLE_FACTORS 1878 108_1878_C_F_WT_50 Sex:F | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__108_1878_C_F_WT_50 SUBJECT_SAMPLE_FACTORS 1879 116_1879_C_F_128_15 Sex:F | Genotype:128 | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__116_1879_C_F_128_15 SUBJECT_SAMPLE_FACTORS 1884 33_1884_C_F_WT_50 Sex:F | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__33_1884_C_F_WT_50 SUBJECT_SAMPLE_FACTORS 1885 114_1885_C_F_128_5 Sex:F | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__114_1885_C_F_128_5 SUBJECT_SAMPLE_FACTORS 1886 93_1886_C_F_WT_5 Sex:F | Genotype:WT | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__93_1886_C_F_WT_5 SUBJECT_SAMPLE_FACTORS 1887 117_1887_C_F_WT_0 Sex:F | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__117_1887_C_F_WT_0 SUBJECT_SAMPLE_FACTORS 1891 39_1891_C_F_128_50 Sex:F | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__39_1891_C_F_128_50 SUBJECT_SAMPLE_FACTORS 1892 99_1892_A_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__99_1892_A_M_WT_0 SUBJECT_SAMPLE_FACTORS 1893 90_1893_C_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__90_1893_C_F_128_0 SUBJECT_SAMPLE_FACTORS 1894 75_1894_A_F_WT_0 Sex:F | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__75_1894_A_F_WT_0 SUBJECT_SAMPLE_FACTORS 1895 47_1895_C_M_WT_15 Sex:M | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__47_1895_C_M_WT_15 SUBJECT_SAMPLE_FACTORS 1896 45_1896_C_M_128_15 Sex:M | Genotype:128 | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__45_1896_C_M_128_15 SUBJECT_SAMPLE_FACTORS 1898 57_1898_A_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__57_1898_A_M_WT_50 SUBJECT_SAMPLE_FACTORS 1899 132_1899_C_M_128_50 Sex:M | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__132_1899_C_M_128_50 SUBJECT_SAMPLE_FACTORS 1901 72_1901_C_M_128_5 Sex:M | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__72_1901_C_M_128_5 SUBJECT_SAMPLE_FACTORS 1903 101_1903_C_M_128_0 Sex:M | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__101_1903_C_M_128_0 SUBJECT_SAMPLE_FACTORS 1904 58_1904_C_M_128_15 Sex:M | Genotype:128 | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__58_1904_C_M_128_15 SUBJECT_SAMPLE_FACTORS 1905 24_1905_A_F_WT_0 Sex:F | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__24_1905_A_F_WT_0 SUBJECT_SAMPLE_FACTORS 1906 3_1906_A_F_WT_50 Sex:F | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__3_1906_A_F_WT_50 SUBJECT_SAMPLE_FACTORS 1907 42_1907_C_F_128_5 Sex:F | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__42_1907_C_F_128_5 SUBJECT_SAMPLE_FACTORS 1908 49_1908_A_F_WT_0 Sex:F | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__49_1908_A_F_WT_0 SUBJECT_SAMPLE_FACTORS 1909 7_1909_C_F_128_15 Sex:F | Genotype:128 | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__7_1909_C_F_128_15 SUBJECT_SAMPLE_FACTORS 1910 4_1910_A_F_WT_50 Sex:F | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__4_1910_A_F_WT_50 SUBJECT_SAMPLE_FACTORS 1911 136_1911_A_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__136_1911_A_M_WT_0 SUBJECT_SAMPLE_FACTORS 1912 96_1912_A_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__96_1912_A_M_WT_50 SUBJECT_SAMPLE_FACTORS 1913 20_1913_A_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__20_1913_A_M_WT_0 SUBJECT_SAMPLE_FACTORS 1914 46_1914_C_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__46_1914_C_M_WT_0 SUBJECT_SAMPLE_FACTORS 1915 70_1915_C_M_128_50 Sex:M | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__70_1915_C_M_128_50 SUBJECT_SAMPLE_FACTORS 1916 43_1916_A_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__43_1916_A_M_WT_50 SUBJECT_SAMPLE_FACTORS 1917 71_1917_A_M_128_0 Sex:M | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__71_1917_A_M_128_0 SUBJECT_SAMPLE_FACTORS 1918 115_1918_A_F_WT_50 Sex:F | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__115_1918_A_F_WT_50 SUBJECT_SAMPLE_FACTORS 1919 88_1919_C_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__88_1919_C_F_128_0 SUBJECT_SAMPLE_FACTORS 1920 62_1920_C_F_128_5 Sex:F | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__62_1920_C_F_128_5 SUBJECT_SAMPLE_FACTORS 1921 10_1921_C_F_128_15 Sex:F | Genotype:128 | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__10_1921_C_F_128_15 SUBJECT_SAMPLE_FACTORS 1922 122_1922_A_F_WT_0 Sex:F | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__122_1922_A_F_WT_0 SUBJECT_SAMPLE_FACTORS 1923 60_1923_C_F_128_50 Sex:F | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__60_1923_C_F_128_50 SUBJECT_SAMPLE_FACTORS 1924 27_1924_C_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__27_1924_C_M_WT_0 SUBJECT_SAMPLE_FACTORS 1928 134_1928_A_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__134_1928_A_F_128_0 SUBJECT_SAMPLE_FACTORS 1929 25_1929_A_F_WT_50 Sex:F | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__25_1929_A_F_WT_50 SUBJECT_SAMPLE_FACTORS 1930 65_1930_C_M_128_15 Sex:M | Genotype:128 | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__65_1930_C_M_128_15 SUBJECT_SAMPLE_FACTORS 1931 6_1931_C_M_WT_15 Sex:M | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__6_1931_C_M_WT_15 SUBJECT_SAMPLE_FACTORS 1933 119_1933_C_F_WT_0 Sex:F | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__119_1933_C_F_WT_0 SUBJECT_SAMPLE_FACTORS 1935 74_1935_A_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__74_1935_A_M_WT_0 SUBJECT_SAMPLE_FACTORS 1936 29_1936_A_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__29_1936_A_M_WT_50 SUBJECT_SAMPLE_FACTORS 1937 92_1937_A_F_128_50 Sex:F | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__92_1937_A_F_128_50 SUBJECT_SAMPLE_FACTORS 1938 97_1938_A_M_128_50 Sex:M | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__97_1938_A_M_128_50 SUBJECT_SAMPLE_FACTORS 1939 105_1939_A_F_128_50 Sex:F | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__105_1939_A_F_128_50 SUBJECT_SAMPLE_FACTORS 1943 121_1943_A_F_WT_50 Sex:F | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__121_1943_A_F_WT_50 SUBJECT_SAMPLE_FACTORS 1944 31_1944_A_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__31_1944_A_M_WT_0 SUBJECT_SAMPLE_FACTORS 1945 137_1945_A_M_128_0 Sex:M | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__137_1945_A_M_128_0 SUBJECT_SAMPLE_FACTORS 1946 138_1946_A_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__138_1946_A_M_WT_50 SUBJECT_SAMPLE_FACTORS 1948 139_1948_A_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__139_1948_A_M_WT_0 SUBJECT_SAMPLE_FACTORS 1949 140_1949_A_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__140_1949_A_F_128_0 SUBJECT_SAMPLE_FACTORS 1950 141_1950_A_F_128_50 Sex:F | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__141_1950_A_F_128_50 SUBJECT_SAMPLE_FACTORS 1951 48_1951_A_F_WT_0 Sex:F | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__48_1951_A_F_WT_0 SUBJECT_SAMPLE_FACTORS 1953 103_1953_A_M_128_50 Sex:M | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__103_1953_A_M_128_50 SUBJECT_SAMPLE_FACTORS 1954 126_1954_C_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__126_1954_C_F_128_0 SUBJECT_SAMPLE_FACTORS 1955 52_1955_C_F_WT_0 Sex:F | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__106_1956_C_M_WT_0 SUBJECT_SAMPLE_FACTORS 1957 35_1957_C_M_WT_5 Sex:M | Genotype:WT | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__35_1957_C_M_WT_5 SUBJECT_SAMPLE_FACTORS 1958 8_1958_C_M_128_5 Sex:M | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__8_1958_C_M_128_5 SUBJECT_SAMPLE_FACTORS 1959 118_1959_C_M_WT_15 Sex:M | Genotype:WT | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__118_1959_C_M_WT_15 SUBJECT_SAMPLE_FACTORS 1960 63_1960_C_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__63_1960_C_M_WT_50 SUBJECT_SAMPLE_FACTORS 1961 120_1961_A_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__120_1961_A_M_WT_0 SUBJECT_SAMPLE_FACTORS 1963 40_1963_C_F_128_5 Sex:F | Genotype:128 | Treatment Dosage:5 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__40_1963_C_F_128_5 SUBJECT_SAMPLE_FACTORS 1964 9_1964_C_F_128_15 Sex:F | Genotype:128 | Treatment Dosage:15 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__9_1964_C_F_128_15 SUBJECT_SAMPLE_FACTORS 1965 36_1965_C_F_128_50 Sex:F | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__36_1965_C_F_128_50 SUBJECT_SAMPLE_FACTORS 1966 14_1966_A_M_128_0 Sex:M | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__14_1966_A_M_128_0 SUBJECT_SAMPLE_FACTORS 1967 15_1967_A_M_128_50 Sex:M | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__15_1967_A_M_128_50 SUBJECT_SAMPLE_FACTORS 1970 81_1970_A_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__81_1970_A_M_WT_50 SUBJECT_SAMPLE_FACTORS 1971 13_1971_A_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__13_1971_A_M_WT_50 SUBJECT_SAMPLE_FACTORS 1972 98_1972_A_M_128_0 Sex:M | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__98_1972_A_M_128_0 SUBJECT_SAMPLE_FACTORS 1973 51_1973_A_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__51_1973_A_M_WT_0 SUBJECT_SAMPLE_FACTORS 1974 12_1974_A_M_128_50 Sex:M | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__12_1974_A_M_128_50 SUBJECT_SAMPLE_FACTORS 1976 18_1976_A_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__18_1976_A_F_128_0 SUBJECT_SAMPLE_FACTORS 1978 67_1978_A_M_128_50 Sex:M | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__67_1978_A_M_128_50 SUBJECT_SAMPLE_FACTORS 1979 83_1979_A_M_WT_50 Sex:M | Genotype:WT | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__83_1979_A_M_WT_50 SUBJECT_SAMPLE_FACTORS 1980 109_1980_A_M_128_0 Sex:M | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__109_1980_A_M_128_0 SUBJECT_SAMPLE_FACTORS 1981 53_1981_A_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__53_1981_A_M_WT_0 SUBJECT_SAMPLE_FACTORS 1982 2_1982_A_M_128_50 Sex:M | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__2_1982_A_M_128_50 SUBJECT_SAMPLE_FACTORS 1983 17_1983_A_M_128_0 Sex:M | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__17_1983_A_M_128_0 SUBJECT_SAMPLE_FACTORS 1984 11_1984_A_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__11_1984_A_F_128_0 SUBJECT_SAMPLE_FACTORS 1985 19_1985_A_F_128_50 Sex:F | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__19_1985_A_F_128_50 SUBJECT_SAMPLE_FACTORS 1986 16_1986_A_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__16_1986_A_F_128_0 SUBJECT_SAMPLE_FACTORS 1987 34_1987_A_F_128_50 Sex:F | Genotype:128 | Treatment Dosage:50 RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__34_1987_A_F_128_50 SUBJECT_SAMPLE_FACTORS 1890 44_1890_A_F_128_0 Sex:F | Genotype:128 | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__44_1890_A_F_128_0 SUBJECT_SAMPLE_FACTORS 1956 106_1956_C_M_WT_0 Sex:M | Genotype:WT | Treatment Dosage:- RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__106_1956_C_M_WT_0 #COLLECTION CO:COLLECTION_SUMMARY For the single injection exposure, mice were sacrificed either 0, 1, 4, 12 or 24 CO:COLLECTION_SUMMARY hours after the injection to examine acute changes to Mn. For 1-week and 20-week CO:COLLECTION_SUMMARY Mn exposures, mice were sacrificed 24 hours after the final injection. For all CO:COLLECTION_SUMMARY studies, mice were sacrificed by cervical dislocation without anesthesia. Mice CO:COLLECTION_SUMMARY were decapitated with sharp scissors and the brain removed. The striatum was CO:COLLECTION_SUMMARY dissected out under a dissecting microscope, flash-frozen in liquid nitrogen and CO:COLLECTION_SUMMARY stored at -80C until later analysis. CO:SAMPLE_TYPE Brain #TREATMENT TR:TREATMENT_SUMMARY This study utilized three Mn exposure paradigms: 1) a single subcutaneous TR:TREATMENT_SUMMARY exposure of either a 1% solution of MnCl2•4(H2O) in filtered MilliQ water at TR:TREATMENT_SUMMARY 50 mg/kg body weight or vehicle (filtered water), 2) a 1-week, and 3) 20-week TR:TREATMENT_SUMMARY chronic exposure. The 1-week exposure is a previously published week-long TR:TREATMENT_SUMMARY exposure protocol (intermittent injections given on days 0, 3 and 6, with TR:TREATMENT_SUMMARY sacrifice on day 7) known to elevate brain Mn levels1. The 20-week exposure is a TR:TREATMENT_SUMMARY twice weekly injection (Mondays and Thursdays) based on the estimated half-life TR:TREATMENT_SUMMARY of 3.5 days for Mn administered via this route and pilot work suggesting the TR:TREATMENT_SUMMARY absence of observable toxicity with this exposure paradigm (including no weight TR:TREATMENT_SUMMARY loss or physical signs of distress). TR:TREATMENT_DOSE 0, 5, 15, 50 mg/kg body weight TR:TREATMENT_COMPOUND Mn #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Previously frozen striatal tissue was lysed in 400µL ice-cold lysing buffer SP:SAMPLEPREP_SUMMARY (1:1:2, ACN:MeOH:Ammonium Bicarbonate (0.1M, pH 8.0) (LC-MS grade). Individual SP:SAMPLEPREP_SUMMARY samples were sonicated using a probe tip sonicator, 10 pulses, at 30% power and SP:SAMPLEPREP_SUMMARY cooled down on ice between samples. A BCA protein assay was used to determine SP:SAMPLEPREP_SUMMARY the protein concentration for each individual sample, and adjusted to a total SP:SAMPLEPREP_SUMMARY amount of protein of 200µg in 200 µL of lysis buffer. Isotopically labeled SP:SAMPLEPREP_SUMMARY standards, Creatinine-D3 and Lysine-D4, were added to each sample to assess SP:SAMPLEPREP_SUMMARY sample processing steps (metabolite extraction and reconstitution). Following SP:SAMPLEPREP_SUMMARY lysis and standard addition, protein precipitation was performed by adding SP:SAMPLEPREP_SUMMARY 800µL of ice-cold methanol (4x by volume). Samples were incubated at -80°C SP:SAMPLEPREP_SUMMARY overnight. Following incubation, samples were centrifuged at 10,000 rpm for 10 SP:SAMPLEPREP_SUMMARY min to eliminate proteins. The supernatants containing metabolites were dried SP:SAMPLEPREP_SUMMARY via speed-vacuum. Dried metabolite extracts were stored frozen at -80C until SP:SAMPLEPREP_SUMMARY ready to use. Prior to mass spectrometry analysis, extracts were reconstituted SP:SAMPLEPREP_SUMMARY in 100 μl of acetonitrile/ water (80:20, v/v) and centrifuged for 5 min at 15K SP:SAMPLEPREP_SUMMARY rpm to remove insoluble material. Quality control samples were prepared by SP:SAMPLEPREP_SUMMARY pooling equal volumes of each sample. Isotopically labeled standards, Valine-D8 SP:SAMPLEPREP_SUMMARY and Inosine-4N15, were added to each sample to determine MS instrument SP:SAMPLEPREP_SUMMARY reproducibility. SP:SAMPLEPREP_PROTOCOL_FILENAME Codreags00_20200327_075634_PR_MS_Metabolomics_Protocol.pdf SP:PROCESSING_STORAGE_CONDITIONS -80℃ SP:EXTRACTION_METHOD Following lysis and standard addition, protein precipitation was performed by SP:EXTRACTION_METHOD adding 800µL of ice-cold methanol (4x by volume). Samples were incubated at SP:EXTRACTION_METHOD -80°C overnight. Following incubation, samples were centrifuged at 10,000 rpm SP:EXTRACTION_METHOD for 10 min to eliminate proteins. The supernatants containing metabolites were SP:EXTRACTION_METHOD dried via speed-vacuum. SP:EXTRACT_STORAGE -80℃ SP:SAMPLE_RESUSPENSION 100 μl of acetonitrile/ water (80:20, v/v) and centrifuged for 5 min at 15K rpm SP:SAMPLE_RESUSPENSION to remove insoluble material SP:SAMPLE_SPIKING Valine-D8 and Inosine-4N15, were added to each sample to determine MS instrument SP:SAMPLE_SPIKING reproducibility. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Vanquish UHPLC binary system CH:COLUMN_NAME SeQuant ZIC-HILIC 3.5-µm, 2.1 mm × 100 mm column CH:FLOW_RATE 0.2 mL/min CH:COLUMN_TEMPERATURE 40 CH:SOLVENT_A 5mM Ammonium formate in 90% water, 10% acetonitrile CH:SOLVENT_B 5mM Ammonium formate in 90% acetonitrile, 10% water #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE Codreags00_20200327_075634_PR_MS_Metabolomics_Protocol.pdf #MS MS:INSTRUMENT_NAME Thermo Q Exactive HF hybrid Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS MS:MS_COMMENTS and UPLC-MS) raw data were imported, processed, normalized and reviewed using MS:MS_COMMENTS Progenesis QI v.2.1 (Non-linear Dynamics, Newcastle, UK). MS:MS_RESULTS_FILE ST001333_AN002222_Results.txt UNITS:peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END