#METABOLOMICS WORKBENCH Mette_Hedemann_20210312_040941 DATATRACK_ID:2524 STUDY_ID:ST001722 ANALYSIS_ID:AN002806 PROJECT_ID:PR001104
VERSION             	1
CREATED_ON             	March 17, 2021, 9:25 am
#PROJECT
PR:PROJECT_TITLE                 	The effects of birth weight and breeding value for protein deposition on the
PR:PROJECT_TITLE                 	plasma metabolome in growing pigs
PR:PROJECT_SUMMARY               	An experiment was conducted with growing pigs, to determine the effects of birth
PR:PROJECT_SUMMARY               	weight (BiW) and estimated breeding value for protein deposition (EBV) on the
PR:PROJECT_SUMMARY               	metabolomic profile in plasma samples collected under different dietary
PR:PROJECT_SUMMARY               	regimens: protein adequate (A) or protein restricted (R, 70% of A).
PR:INSTITUTE                     	Aarhus University
PR:DEPARTMENT                    	Animal Science
PR:LABORATORY                    	Metabolomics LC-MS platform Aarhus University Foulum
PR:LAST_NAME                     	Hedemann
PR:FIRST_NAME                    	Mette
PR:ADDRESS                       	Blichers Alle 20, Tjele, -, 8830, Denmark
PR:EMAIL                         	Mette.Hedemann@anis.au.dk
PR:PHONE                         	51448783
PR:FUNDING_SOURCE                	European Union's H2020 Program
PR:PROJECT_COMMENTS              	Feed-a-Gene, Grant agreement no 633531
PR:PUBLICATIONS                  	The effects of birth weight and breeding value for protein deposition on
PR:PUBLICATIONS                  	nitrogen efficiency in growing pigs
PR:CONTRIBUTORS                  	C.M.C. van der Peet-Schwering, L.M.G. Verschuren, R. Bergsma, M.S. Hedemann,
PR:CONTRIBUTORS                  	G.P. Binnendijk, A.J.M. Jansman
#STUDY
ST:STUDY_TITLE                   	The effects of birth weight and breeding value for protein deposition on the
ST:STUDY_TITLE                   	plasma metabolome in growing pigs
ST:STUDY_SUMMARY                 	An experiment was conducted with growing pigs, to determine the effects of birth
ST:STUDY_SUMMARY                 	weight (BiW) and estimated breeding value for protein deposition (EBV) on the
ST:STUDY_SUMMARY                 	metabolomic profile in plasma samples collected under different dietary
ST:STUDY_SUMMARY                 	regimens: protein adequate (A) or protein restricted (R, 70% of A).
ST:INSTITUTE                     	Aarhus University
ST:DEPARTMENT                    	Animal Science
ST:LABORATORY                    	Metabolomics LC-MS platform Aarhus University Foulum
ST:LAST_NAME                     	Hedemann
ST:FIRST_NAME                    	Mette
ST:ADDRESS                       	Blichers Alle 20, Tjele, -, 8830, Denmark
ST:EMAIL                         	Mette.Hedemann@anis.au.dk
ST:PHONE                         	51448783
ST:TOTAL_SUBJECTS                	40
ST:NUM_MALES                     	40
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Sus scrofa
SU:TAXONOMY_ID                   	9823
SU:AGE_OR_AGE_RANGE              	0-125 days
SU:WEIGHT_OR_WEIGHT_RANGE        	1-80 kg
SU:GENDER                        	Male
SU:ANIMAL_ANIMAL_SUPPLIER        	Swine Innovation Centre Sterksel, the Netherlands
SU:ANIMAL_HOUSING                	Metabolism cages
SU:ANIMAL_LIGHT_CYCLE            	12h light / 12h darknes
SU:ANIMAL_FEED                   	Experimental diets (see publication)
SU:ANIMAL_WATER                  	Ad libitum
SU:ANIMAL_INCLUSION_CRITERIA     	Birth weight
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	QC	QC-1_neg_1a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_1a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-2_neg_1a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_1a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-3_neg_1a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_1a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-4_neg_1a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_1a.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9001	Plasma_neg_21	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=10.325; Wght_birth=2.05; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_21.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9189	Plasma_neg_22	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=1.003; Wght_birth=1.70; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_22.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9003	Plasma_neg_23	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=9.259; Wght_birth=1.93; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_23.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9294	Plasma_neg_24	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-4.591; Wght_birth=1.89; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_24.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-1_neg_2a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_2a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-2_neg_2a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_2a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-3_neg_2a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_2a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-4_neg_2a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_2a.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9215	Plasma_neg_25	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-4.771; Wght_birth=0.93; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_25.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9096	Plasma_neg_26	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=5.035; Wght_birth=1.04; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_26.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9104	Plasma_neg_27	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=7.067; Wght_birth=1.15; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_27.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9300	Plasma_neg_28	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=0.142; Wght_birth=1.13; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_28.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9150	Plasma_neg_29	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-4.872; Wght_birth=1.95; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_29.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9062	Plasma_neg_30	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=13.209; Wght_birth=0.95; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_30.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9219	Plasma_neg_31	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=0.031; Wght_birth=1.61; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_31.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9221	Plasma_neg_32	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-3.470; Wght_birth=1.10; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_32.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9303	Plasma_neg_33	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=9.738; Wght_birth=1.72; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_33.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9154	Plasma_neg_34	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-0.558; Wght_birth=1.84; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_34.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9325	Plasma_neg_35	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=0.882; Wght_birth=1.16; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_35.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9188	Plasma_neg_36	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=8.689; Wght_birth=1.77; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_36.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9310	Plasma_neg_37	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=16.944; Wght_birth=0.78; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_37.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9006	Plasma_neg_38	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=13.239; Wght_birth=1.77; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_38.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9149	Plasma_neg_39	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-3.794; Wght_birth=1.89; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_39.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9004	Plasma_neg_40	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=18.784; Wght_birth=1.87; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_40.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9001	Plasma_neg_41	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=10.325; Wght_birth=2.05; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_41.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9189	Plasma_neg_42	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=1.003; Wght_birth=1.70; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_42.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9003	Plasma_neg_43	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=9.259; Wght_birth=1.93; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_43.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9294	Plasma_neg_44	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-4.591; Wght_birth=1.89; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_44.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9215	Plasma_neg_45	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-4.771; Wght_birth=0.93; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_45.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9096	Plasma_neg_46	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=5.035; Wght_birth=1.04; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_46.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9104	Plasma_neg_47	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=7.067; Wght_birth=1.15; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_47.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9300	Plasma_neg_48	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=0.142; Wght_birth=1.13; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_48.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-1_neg_3a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_3a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-2_neg_3a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_3a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-3_neg_3a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_3a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-4_neg_3a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_3a.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9150	Plasma_neg_49	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-4.872; Wght_birth=1.95; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_49.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9062	Plasma_neg_50	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=13.209; Wght_birth=0.95; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_50.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9219	Plasma_neg_51	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=0.031; Wght_birth=1.61; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_51.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9221	Plasma_neg_52	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-3.470; Wght_birth=1.10; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_52.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9303	Plasma_neg_53	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=9.738; Wght_birth=1.72; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_53.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9154	Plasma_neg_54	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-0.558; Wght_birth=1.84; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_54.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9325	Plasma_neg_55	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=0.882; Wght_birth=1.16; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_55.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9188	Plasma_neg_56	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=8.689; Wght_birth=1.77; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_56.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9310	Plasma_neg_57	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=16.944; Wght_birth=0.78; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_57.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9006	Plasma_neg_58	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=13.239; Wght_birth=1.77; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_58.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9149	Plasma_neg_59	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-3.794; Wght_birth=1.89; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_59.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9004	Plasma_neg_60	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=18.784; Wght_birth=1.87; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_60.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-1_neg_4a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_4a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-2_neg_4a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_4a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-3_neg_4a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_4a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-4_neg_4a	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_4a.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-1_neg_1	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_1.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-2_neg_1	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_1.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-3_neg_1	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_1.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-4_neg_1	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_1.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9309	Plasma_neg_81	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=14.658; Wght_birth=1.16; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_81.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9034	Plasma_neg_82	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=0.057; Wght_birth=1.16; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_82.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9171	Plasma_neg_83	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-2.490; Wght_birth=1.02; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_83.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9125	Plasma_neg_84	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=10.481; Wght_birth=0.84; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_84.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-1_neg_2	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_2.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-2_neg_2	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_2.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-3_neg_2	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_2.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-4_neg_2	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_2.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9304	Plasma_neg_85	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=14.534; Wght_birth=1.62; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_85.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9407	Plasma_neg_86	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=8.842; Wght_birth=1.01; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_86.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9315	Plasma_neg_87	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=0.820; Wght_birth=1.14; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_87.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9410	Plasma_neg_88	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=14.929; Wght_birth=1.16; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_88.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9305	Plasma_neg_89	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=12.996; Wght_birth=1.65; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_89.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9247	Plasma_neg_90	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-12.409; Wght_birth=1.09; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_90.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9152	Plasma_neg_91	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-2.114; Wght_birth=1.92; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_91.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9296	Plasma_neg_92	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=0.786; Wght_birth=1.86; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_92.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9295	Plasma_neg_93	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-3.789; Wght_birth=1.72; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_93.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9266	Plasma_neg_94	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=7.079; Wght_birth=1.62; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_94.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9187	Plasma_neg_95	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=7.204; Wght_birth=1.95; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_95.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9031	Plasma_neg_96	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=8.422; Wght_birth=1.07; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_96.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9117	Plasma_neg_97	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-2.702; Wght_birth=0.94; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_97.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9121	Plasma_neg_98	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-1.224; Wght_birth=1.01; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_98.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9177	Plasma_neg_99	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=5.137; Wght_birth=1.04; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_99.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9309	Plasma_neg_100	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=14.658; Wght_birth=1.16; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_100.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9034	Plasma_neg_101	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=0.057; Wght_birth=1.16; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_101.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9171	Plasma_neg_102	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-2.490; Wght_birth=1.02; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_102.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9125	Plasma_neg_103	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=10.481; Wght_birth=0.84; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_103.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9304	Plasma_neg_104	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=14.534; Wght_birth=1.62; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_104.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9407	Plasma_neg_105	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=8.842; Wght_birth=1.01; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_105.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9315	Plasma_neg_107	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=0.820; Wght_birth=1.14; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_107.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9410	Plasma_neg_108	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=14.929; Wght_birth=1.16; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_108.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-1_neg_3	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_3.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-2_neg_3	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_3.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-3_neg_3	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_3.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-4_neg_3	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_3.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9305	Plasma_neg_109	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=12.996; Wght_birth=1.65; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_109.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9247	Plasma_neg_110	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-12.409; Wght_birth=1.09; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_110.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9152	Plasma_neg_111	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-2.114; Wght_birth=1.92; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_111.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9296	Plasma_neg_112	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=0.786; Wght_birth=1.86; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_112.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9295	Plasma_neg_113	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-3.789; Wght_birth=1.72; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_113.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9266	Plasma_neg_114	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=7.079; Wght_birth=1.62; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_114.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9187	Plasma_neg_115	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=7.204; Wght_birth=1.95; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_115.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9031	Plasma_neg_116	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=8.422; Wght_birth=1.07; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_116.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9117	Plasma_neg_117	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-2.702; Wght_birth=0.94; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_117.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9121	Plasma_neg_118	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-1.224; Wght_birth=1.01; Breedvalue_cat=low; RAW_FILE_NAME=Plasma_neg_118.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9177	Plasma_neg_119	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=5.137; Wght_birth=1.04; Breedvalue_cat=high; RAW_FILE_NAME=Plasma_neg_119.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-1_neg_4	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_4.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-2_neg_4	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_4.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-3_neg_4	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_4.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC-4_neg_4	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Wght_birth=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_4.mzXML
#COLLECTION
CO:COLLECTION_SUMMARY            	At the end of each feeding regime, blood samples were collected from the jugular
CO:COLLECTION_SUMMARY            	vein. Per sampling moment, two 9-mL plasma tubes (Vacuette, Greiner Bio-One,
CO:COLLECTION_SUMMARY            	Kremsmünster, Austria) per pig were filled and allowed to clot for 1 h at room
CO:COLLECTION_SUMMARY            	temperature. Plasma was collected after centrifugation for 15 min at 2,000 ∙ g
CO:COLLECTION_SUMMARY            	and was stored at -80°C pending analyses.
CO:SAMPLE_TYPE                   	Blood (plasma)
CO:COLLECTION_METHOD             	Jugular vein puncture
CO:COLLECTION_FREQUENCY          	At the end of feeding period
CO:VOLUMEORAMOUNT_COLLECTED      	2 x 9 ml blood
CO:STORAGE_CONDITIONS            	-80℃
CO:COLLECTION_VIALS              	Vacuette
#TREATMENT
TR:TREATMENT_SUMMARY             	At an age of 14 weeks, 10 LBW-LEBV (BiW: 1.07 + 0.09 kg; EBV: -2.52 + 3.97 g/d,
TR:TREATMENT_SUMMARY             	compared to an average crossbred pig with a protein deposition of 165 g/d), 10
TR:TREATMENT_SUMMARY             	LBW-HEBV (BiW: 1.02 + 0.13 kg; EBV: 10.47 + 4.26 g/d), 10 HBW-LEBV (BiW: 1.80 +
TR:TREATMENT_SUMMARY             	0.13 kg; EBV: - 2.15 + 2.28 g/d), and 10 HBW-HEBV (BiW: 1.80 + 0.15 kg; EBV:
TR:TREATMENT_SUMMARY             	11.18 + 3.68 g/d), male growing pigs (Synthetic boar x (Dutch Landrace x Large
TR:TREATMENT_SUMMARY             	White)) were allotted to the experiment. The pigs were individually housed in
TR:TREATMENT_SUMMARY             	metabolism cages (1.80 x 0.80 m) at a room temperature of 22oC. They were
TR:TREATMENT_SUMMARY             	subjected to N- balance measurements in two sequential periods of 5 d using a
TR:TREATMENT_SUMMARY             	restricted feeding regime. After a 6-d adaptation period to the metabolism
TR:TREATMENT_SUMMARY             	cages, pigs were adapted for 5 days to the experimental diets before the start
TR:TREATMENT_SUMMARY             	of the first 5-d balance period. Pigs were assigned to a protein adequate (A) or
TR:TREATMENT_SUMMARY             	protein restricted (R, 70% of A) regime in a change-over design.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Plasma samples (150 µL) were pipetted into a 96-well plate and 450 µL
SP:SAMPLEPREP_SUMMARY            	acetonitrile containing an internal standard mix of glycocholic acid
SP:SAMPLEPREP_SUMMARY            	(glycine-1-13C) and p-chlorophenylalanine to a final concentration of 0.01
SP:SAMPLEPREP_SUMMARY            	mg/mL, was added per well. The samples were mixed immediately and placed at 4°C
SP:SAMPLEPREP_SUMMARY            	for 10 min for protein precipitation. After centrifugation (3700 rpm for 25 min
SP:SAMPLEPREP_SUMMARY            	at 4°C), the supernatant was transferred to a filter plate fixed on top of a
SP:SAMPLEPREP_SUMMARY            	96-well collection plate in a manifold with a pressure gauge. Vacuum was then
SP:SAMPLEPREP_SUMMARY            	applied to the filter plate, and the solvent containing plasma components was
SP:SAMPLEPREP_SUMMARY            	collected into the 96-well collection plate. The collection plate was placed in
SP:SAMPLEPREP_SUMMARY            	a vacuum centrifuge and the samples were evaporated to dryness (ca. 2.5 h, 805
SP:SAMPLEPREP_SUMMARY            	× g and 30°C). A volume of 150 µL of water/acetonitrile/formic acid
SP:SAMPLEPREP_SUMMARY            	(95/5/0.1%) was added to the dried extracts in the collection plate. The dried
SP:SAMPLEPREP_SUMMARY            	collection plate was then sealed with a piece of aluminum laminate (#186002789,
SP:SAMPLEPREP_SUMMARY            	Waters), centrifuged (3700 rpm for 25 min at 4°C), and kept in the auto-sampler
SP:SAMPLEPREP_SUMMARY            	at 10°C for the UPLC-QTOF/MS analysis. The injection volume was 3 µl.
SP:PROCESSING_STORAGE_CONDITIONS 	-80℃
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Chromatographic separation was performed on a Dionex UltiMate 3000RSL Binary
CH:CHROMATOGRAPHY_SUMMARY        	UHPLC System (Thermo Scientific Dionex, Sunnyvale, CA) equipped with a HSS T3
CH:CHROMATOGRAPHY_SUMMARY        	C18 UHPLC column, 1.8 µm, 100x2.1 mm (Waters Corporation, Milford, MA). The
CH:CHROMATOGRAPHY_SUMMARY        	column was maintained at 30°C. Samples were kept in the autosampler at 10°C,
CH:CHROMATOGRAPHY_SUMMARY        	and the injection volume for both sample types was 3 µl. The mobile phases were
CH:CHROMATOGRAPHY_SUMMARY        	0.1% formic acid in Milli-Q water (A) and 0.1% formic acid in acetonitrile (B).
CH:CHROMATOGRAPHY_SUMMARY        	The gradient program for the plasma samples was as follows: 0-12 min, linear
CH:CHROMATOGRAPHY_SUMMARY        	gradient from 5-100% B; 12-13 min, 100% B and return to initial conditions in
CH:CHROMATOGRAPHY_SUMMARY        	0.2 min. In all cases, the column was re-equilibrated at 5% B for 2 min at the
CH:CHROMATOGRAPHY_SUMMARY        	beginning of each run. The flow rate was 0.4 ml/min
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Dionex Ultimate 3000 RS
CH:COLUMN_NAME                   	Waters Acquity BEH HSS T3 (100 x 2.1mm, 1.8um)
CH:FLOW_RATE                     	0.4 ml/min
CH:COLUMN_TEMPERATURE            	30
CH:SOLVENT_A                     	0.1% formic acid in water
CH:SOLVENT_B                     	0.1% formic acid in acetonitrile
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Bruker Impact HD
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	The eluent was introduced into the mass spectrometer by electrospray ionization,
MS:MS_COMMENTS                   	with capillary set in the positive and negative mode to 4500 and 3600 V,
MS:MS_COMMENTS                   	respectively. End-plate offset voltage was set to 500 V. Nitrogen was used as
MS:MS_COMMENTS                   	both nebulizer and drying gas with a gas pressure of 1.8 bar. Drying gas
MS:MS_COMMENTS                   	temperature and flow were 200 °C and 8.0 L/min, respectively. Spectra were
MS:MS_COMMENTS                   	acquired over the scan range of 50−1000 m/z. A solution of lithium formate
MS:MS_COMMENTS                   	clusters (5 mM) (water/isopropanol/formic acid in a 50:50:0.2 v/v/v ratio) was
MS:MS_COMMENTS                   	injected prior to each chromatographic run as an external calibrant.
MS:MS_RESULTS_FILE               	ST001722_AN002806_Results.txt	UNITS:peak area	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END