#METABOLOMICS WORKBENCH tnuriel_20210602_131536_mwtab.txt DATATRACK_ID:2670 STUDY_ID:ST001820 ANALYSIS_ID:AN002955 PROJECT_ID:000000
VERSION             	1
CREATED_ON             	June 7, 2021, 12:36 pm
#PROJECT
PR:PROJECT_TYPE                  	Lipidomics analysis
PR:PROJECT_SUMMARY               	Apolipoprotein E ε4 (APOE4) is the primary genetic risk factor for the
PR:PROJECT_SUMMARY               	late-onset form of Alzheimer's disease (AD). Although the reason for this
PR:PROJECT_SUMMARY               	association is not completely understood, researchers have uncovered numerous
PR:PROJECT_SUMMARY               	effects of APOE4 expression on AD-relevant brain processes, including amyloid
PR:PROJECT_SUMMARY               	beta (Aβ) accumulation, lipid metabolism, endosomal-lysosomal processing and
PR:PROJECT_SUMMARY               	bioenergetics. In this study, we aimed to determine the effect of APOE4 allelic
PR:PROJECT_SUMMARY               	dosage on regional brain lipid composition in aged mice, as well as in cultured
PR:PROJECT_SUMMARY               	neurons. We performed a targeted lipidomic analysis on the entorhinal cortex
PR:PROJECT_SUMMARY               	(EC) and primary visual cortex (PVC) from 14–15 month-old APOE3/3, APOE3/4,
PR:PROJECT_SUMMARY               	and APOE4/4 targeted replacement mice, as well as on WT neurons cultured with
PR:PROJECT_SUMMARY               	conditioned media from APOE3/3 or APOE4/4 astrocytes. Our results reveal that
PR:PROJECT_SUMMARY               	the EC possesses increased susceptibility to APOE4-associated lipid alterations
PR:PROJECT_SUMMARY               	compared to the PVC. In the EC, APOE4 expression showed a dominant effect in
PR:PROJECT_SUMMARY               	decreasing diacylglycerol (DAG) levels, and a semi-dominant additive effect in
PR:PROJECT_SUMMARY               	the upregulation of multiple ceramide, glycosylated sphingolipid and
PR:PROJECT_SUMMARY               	bis(monoacylglycerol)phosphate (BMP) species, lipids known to accumulate as a
PR:PROJECT_SUMMARY               	result of endosomal-lysosomal dysfunction and defective lysosomal clearance.
PR:PROJECT_SUMMARY               	Neurons treated with conditioned media from APOE4 vs. APOE3 astrocytes also
PR:PROJECT_SUMMARY               	showed similar alterations of DAG and BMP species as those observed in the mouse
PR:PROJECT_SUMMARY               	EC. Our results suggest that APOE4 expression differentially modulates regional
PR:PROJECT_SUMMARY               	and neuronal lipid signatures, which may underlie the increased susceptibility
PR:PROJECT_SUMMARY               	of EC-localized neurons to AD pathology.
PR:INSTITUTE                     	Columbia University
PR:FIRST_NAME                    	Tal
PR:ADDRESS                       	630 W 168th St., P&S 12-430
PR:EMAIL                         	tn2283@cumc.columbia.edu
PR:PHONE                         	2123045683
PR:PROJECT_TITLE                 	APOE4-associated differences in lipidomics signatures in mouse brain and
PR:PROJECT_TITLE                 	cultured neurons
PR:LAST_NAME                     	Nuriel
#STUDY
ST:STUDY_TITLE                   	WT neurons treated with APOE3/3 and APOE4/4 ACM
ST:STUDY_SUMMARY                 	We performed a targeted lipidomic analysis on WT neurons treated with astrocyte
ST:STUDY_SUMMARY                 	conditioned media (ACM) from APOE3/3 or APOE4/4 astrocytes.
ST:INSTITUTE                     	Columbia University
ST:LAST_NAME                     	Nuriel
ST:FIRST_NAME                    	Tal
ST:ADDRESS                       	630 W 168th St., P&S 12-430
ST:EMAIL                         	tn2283@cumc.columbia.edu
ST:PHONE                         	2123045683
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENDER                        	Not applicable
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	1	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	2	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	3	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	4	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	5	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	6	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	7	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	8	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	9	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	10	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	11	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	12	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	13	Condition:APOE4/4 ACM	
SUBJECT_SAMPLE_FACTORS           	-	14	Condition:APOE4/4 ACM	
SUBJECT_SAMPLE_FACTORS           	-	15	Condition:APOE4/4 ACM	
SUBJECT_SAMPLE_FACTORS           	-	16	Condition:APOE4/4 ACM	
SUBJECT_SAMPLE_FACTORS           	-	17	Condition:APOE4/4 ACM	
SUBJECT_SAMPLE_FACTORS           	-	18	Condition:APOE4/4 ACM	
#COLLECTION
CO:COLLECTION_SUMMARY            	Astrocyte conditioned media (ACM) was obtained from immortalized astrocyte cell
CO:COLLECTION_SUMMARY            	lines (a gift from Dr. David Holtzman) that were originally generated from
CO:COLLECTION_SUMMARY            	primary astrocytes from P1-2 pups of APOE targeted replacement mice (42). The
CO:COLLECTION_SUMMARY            	immortalized astrocytes were conditioned with Neurobasal media supplemented with
CO:COLLECTION_SUMMARY            	B27, Glutamax-I, Normocin and 1% penicillin/streptomycin for 24 hours. This ACM
CO:COLLECTION_SUMMARY            	was then collected, stored at -80ºC, and thawed prior to use. WT primary
CO:COLLECTION_SUMMARY            	cortical neuronal cultures were obtained from embryonic day 17 (E17) C57Bl/6
CO:COLLECTION_SUMMARY            	embryos. Briefly, pregnant mice were euthanized by cervical dislocation and the
CO:COLLECTION_SUMMARY            	embryo brains extracted. The meninges were carefully stripped off and the
CO:COLLECTION_SUMMARY            	cortices dissected out. The cortices were then enzymatically dissociated in
CO:COLLECTION_SUMMARY            	0.25% Trypsin-EDTA and resuspended in Neurobasal media supplemented with B27,
CO:COLLECTION_SUMMARY            	Glutamax-I, Normocin and 1% penicillin/streptomycin. Dissociated cells were
CO:COLLECTION_SUMMARY            	counted and 300K neurons per well were plated directly into ACM in poly-D-lysine
CO:COLLECTION_SUMMARY            	(PDL)-coated 6-well plates. ACM-treated neurons were then incubated at 37⁰C
CO:COLLECTION_SUMMARY            	for 7 days in a humidified chamber with 5% CO2, with 50% media exchange (with
CO:COLLECTION_SUMMARY            	newly thawed ACM) once every 3 days.
CO:SAMPLE_TYPE                   	Neurons
#TREATMENT
TR:TREATMENT_SUMMARY             	WT neurons were incubated for 7 days in either no ACM, APOE3/3 ACM, or APOE4/4
TR:TREATMENT_SUMMARY             	ACM.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Lipid and small-molecule metabolite extraction was performed using a methyl
SP:SAMPLEPREP_SUMMARY            	tert-butyl ether (MTBE)/methanol extraction protocol. Briefly, harvested neurons
SP:SAMPLEPREP_SUMMARY            	were homogenized in ice-cold methanol. Following homogenization, samples were
SP:SAMPLEPREP_SUMMARY            	incubated in 1200 ul of MTBE for 1 hr at room temperature to separate
SP:SAMPLEPREP_SUMMARY            	organic-soluble lipids from aqueous-soluble lipids and other small-molecules.
SP:SAMPLEPREP_SUMMARY            	Finally, 360 ul of ultrapure water was added (for a final ratio of 3:1:0.9
SP:SAMPLEPREP_SUMMARY            	MTBE:methanol:water) to resolve the two liquid phases, and each samples were
SP:SAMPLEPREP_SUMMARY            	centrifuged at 10,000 x g for 10 min. For this experiment, the upper organic
SP:SAMPLEPREP_SUMMARY            	phase was collected from each sample and stored in a separate tube, and the
SP:SAMPLEPREP_SUMMARY            	remaining protein pellets were resuspended in 25 mM ammonium bicarbonate, pH 8,
SP:SAMPLEPREP_SUMMARY            	with 2.5% SDS. A BCA protein assay was performed on each protein fraction, and
SP:SAMPLEPREP_SUMMARY            	the organic phase was normalized to their protein concentration equivalent with
SP:SAMPLEPREP_SUMMARY            	100% methanol. All samples were then stored at -80°C prior to analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Reverse phase
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1260
CH:COLUMN_NAME                   	Agilent Eclipse XDB-C18 (100 x 3.0mm)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6490 QQQ
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Lipid profiling was performed using an Agilent 1260 HPLC coupled to an Agilent
MS:MS_COMMENTS                   	6490 triple quadrupole (QQQ) mass spectrometer (41). Each sample was run through
MS:MS_COMMENTS                   	three separate chromatographic conditions (reverse-phase negative mode,
MS:MS_COMMENTS                   	reverse-phase positive mode and normal-phase positive mode) for the effective
MS:MS_COMMENTS                   	quantification of over 337 distinct lipids from over 28 lipid subclasses, as
MS:MS_COMMENTS                   	previously described (41). Individual lipid species were measured by multiple
MS:MS_COMMENTS                   	reaction monitoring transitions.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	area under the curve
MS_METABOLITE_DATA_START
Samples	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Factors	Condition:No ACM	Condition:No ACM	Condition:No ACM	Condition:No ACM	Condition:No ACM	Condition:No ACM	Condition:APOE3/3 ACM	Condition:APOE3/3 ACM	Condition:APOE3/3 ACM	Condition:APOE3/3 ACM	Condition:APOE3/3 ACM	Condition:APOE3/3 ACM	Condition:APOE4/4 ACM	Condition:APOE4/4 ACM	Condition:APOE4/4 ACM	Condition:APOE4/4 ACM	Condition:APOE4/4 ACM	Condition:APOE4/4 ACM
Cer d18:1/16:0	0.73017	0.57609	0.34963	0.44577	0.28386	0.54156	0.64483	0.34346	0.36564	0.38758	0.46827	0.34631	0.78287	0.57645	0.61650	0.83883	0.98733	0.73438
Cer d18:1/16:1	0.10681	0.09229	0.08725	0.08412	0.07677	0.08197	0.10998	0.09236	0.09426	0.07315	0.07817	0.08482	0.10395	0.09373	0.08893	0.08982	0.08686	0.08399
Cer d18:1/18:0	2.39533	1.54941	0.92958	1.39400	0.85257	1.53768	0.85218	0.50465	0.51728	0.55274	0.65264	0.46479	1.02039	0.65747	0.73230	0.99205	1.03761	0.89678
Cer d18:1/18:1	0.01435	0.01070	0.00623	0.00905	0.00603	0.00978	0.01258	0.00729	0.01005	0.00995	0.01056	0.00837	0.01248	0.00704	0.01019	0.01197	0.01543	0.01385
Cer d18:1/20:0	0.04830	0.03552	0.02205	0.03281	0.02196	0.03319	0.02976	0.01826	0.01965	0.01987	0.02390	0.01593	0.04195	0.02527	0.03028	0.03415	0.03516	0.03308
Cer d18:1/20:1	0.00210	0.00173	0.00128	0.00115	0.00077	0.00143	0.00221	0.00166	0.00189	0.00109	0.00224	0.00151	0.00339	0.00189	0.00210	0.00348	0.00302	0.00189
Cer d18:1/22:0	0.03529	0.02723	0.01612	0.02013	0.01518	0.02470	0.04451	0.02376	0.02706	0.02530	0.02593	0.01944	0.04956	0.02881	0.03098	0.03303	0.03689	0.04134
Cer d18:1/22:1	0.00741	0.00549	0.00307	0.00500	0.00267	0.00465	0.01409	0.00896	0.01066	0.00860	0.00912	0.00677	0.01244	0.00727	0.00876	0.01073	0.00995	0.01107
Cer d18:1/24:0	0.02983	0.01825	0.01362	0.01585	0.01059	0.01630	0.03897	0.02404	0.02798	0.02293	0.02722	0.01935	0.04035	0.02656	0.03355	0.03863	0.03729	0.03958
Cer d18:1/24:1	0.07870	0.05398	0.03755	0.04159	0.02985	0.05053	0.09316	0.05134	0.06739	0.05507	0.06208	0.05627	0.09829	0.06879	0.07232	0.08856	0.07801	0.09821
SM d18:1/16:0	1.05615	1.20233	1.03028	1.10057	0.68982	1.08090	3.27254	1.56607	1.78255	1.99436	2.14729	0.93978	2.18198	1.28287	2.28218	2.20512	2.63729	2.21768
SM d18:1/16:1	0.01133	0.01305	0.01267	0.01419	0.01126	0.01453	0.08025	0.03845	0.04556	0.05755	0.04620	0.02304	0.09933	0.06605	0.10460	0.10503	0.10462	0.08129
SM d18:1/18:0	2.29590	2.11651	1.45786	1.72017	1.01627	1.60009	2.64115	1.36354	1.60577	1.59330	1.77727	0.85666	1.48472	0.94337	1.66839	2.01295	2.41688	1.81281
SM d18:1/18:1	0.06422	0.06689	0.06544	0.06982	0.04440	0.06605	0.14202	0.06734	0.07516	0.07922	0.08535	0.04152	0.10752	0.06313	0.11160	0.10343	0.12959	0.10968
SM d18:1/20:0	0.17117	0.15922	0.10129	0.10515	0.06507	0.09943	0.36209	0.16305	0.17239	0.17623	0.19379	0.08840	0.23150	0.12009	0.19892	0.21858	0.28090	0.19763
SM d18:1/20:1	0.00705	0.00645	0.00510	0.00510	0.00425	0.00536	0.02775	0.01324	0.01172	0.01294	0.01483	0.00684	0.01626	0.01089	0.01549	0.01667	0.01993	0.01449
SM d18:1/22:0	0.17414	0.17863	0.09543	0.11161	1.72077	2.21982	0.49381	0.23171	0.24671	0.28036	0.27384	0.12648	0.29471	0.15943	0.25669	0.29235	0.37694	0.27223
SM d18:1/22:1	0.02142	0.01897	0.01246	0.01476	0.00941	0.01316	0.11373	0.05396	0.05068	0.06047	0.06598	0.03323	0.05470	0.03240	0.05230	0.05749	0.07182	0.04931
SM d18:1/24:0	0.15916	0.14337	0.08306	0.09661	0.05738	0.09118	0.40520	0.20019	0.19962	0.23396	0.22448	0.10816	0.32818	0.16184	0.26211	0.31157	0.41425	0.31159
SM d18:1/24:1	0.25595	0.23856	0.14510	0.14748	0.09482	0.15831	0.84097	0.42030	0.45428	0.48395	0.49529	0.21906	0.62196	0.30303	0.51687	0.62712	0.72444	0.62531
SM d18:1/26:0	0.00117	0.00158	0.00070	0.00093	0.00040	0.00061	0.00355	0.00137	0.00148	0.00159	0.00161	0.00076	0.00342	0.00149	0.00237	0.00323	0.00381	0.00269
SM d18:1/26:1	0.00186	0.00193	0.00102	0.00137	0.00067	0.00138	0.00737	0.00317	0.00315	0.00392	0.00416	0.00158	0.00584	0.00308	0.00507	0.00654	0.00732	0.00585
dhSM d18:0/16:0	0.04385	0.04916	0.03123	0.03206	0.01981	0.00168	0.10140	0.04795	0.05624	0.05786	0.07377	0.03053	0.05089	0.03101	0.05387	0.06215	0.07920	0.05051
dhSM d18:0/18:0	0.08889	0.09752	0.05697	0.05900	0.03375	0.05345	0.12178	0.05572	0.07061	0.06787	0.07498	0.03644	0.06294	0.04058	0.06165	0.07810	0.11202	0.07341
dhSM d18:0/18:1	0.00656	0.00807	0.00484	0.00660	0.00379	0.00455	0.04722	0.02038	0.02191	0.02528	0.02846	0.01044	0.02523	0.01260	0.01927	0.01987	0.02144	0.02039
dhSM d18:0/22:0	0.00260	0.00247	0.00162	0.00194	0.00111	0.00153	0.00696	0.00384	0.00410	0.00466	0.00506	0.00174	0.00472	0.00342	0.00513	0.00431	0.00598	0.00519
dhSM d18:0/24:0	0.00180	0.00210	0.00087	0.00118	0.00067	0.00093	0.00543	0.00244	0.00208	0.00291	0.00310	0.00152	0.00319	0.00176	0.00277	0.00380	0.00483	0.00315
dhSM d18:0/24:1	0.01348	0.01033	0.00533	0.00653	0.00440	0.00701	0.05304	0.02472	0.02333	0.02361	0.02767	0.01296	0.02616	0.01428	0.02445	0.03232	0.04249	0.03190
GalCer d18:0/16:0	0.00181	0.00175	0.00125	0.00144	0.00111	0.00218	0.00376	0.00250	0.00274	0.00289	0.00309	0.00257	0.00284	0.00229	0.00281	0.00367	0.00366	0.00281
GalCer d18:0/16:1	0.00040	0.00032	0.00034	0.00038	0.00025	0.00042	0.00077	0.00050	0.00061	0.00056	0.00072	0.00048	0.00065	0.00049	0.00063	0.00074	0.00079	0.00071
GalCer d18:0/18:0	0.01182	0.01098	0.00706	0.00975	0.00724	0.01256	0.01280	0.00867	0.00960	0.01008	0.01261	0.00921	0.00962	0.00689	0.00863	0.01149	0.01221	0.01027
GalCer d18:0/18:1	0.00316	0.00260	0.00170	0.00236	0.00176	0.00275	0.00294	0.00189	0.00238	0.00211	0.00268	0.00216	0.00221	0.00157	0.00178	0.00222	0.00317	0.00253
GalCer d18:0/20:0	0.00063	0.00070	0.00054	0.00057	0.00056	0.00066	0.00133	0.00090	0.00115	0.00103	0.00123	0.00083	0.00102	0.00053	0.00101	0.00107	0.00112	0.00114
GalCer d18:0/22:0	0.00099	0.00080	0.00066	0.00079	0.00078	0.00091	0.00294	0.00200	0.00228	0.00221	0.00274	0.00198	0.00211	0.00165	0.00176	0.00274	0.00258	0.00251
GalCer d18:0/24:0	0.00068	0.00057	0.00047	0.00045	0.00039	0.00064	0.00155	0.00094	0.00099	0.00103	0.00140	0.00111	0.00172	0.00116	0.00119	0.00185	0.00217	0.00177
GalCer d18:0/24:1	0.00065	0.00056	0.00039	0.00050	0.00043	0.00069	0.00222	0.00138	0.00162	0.00161	0.00229	0.00145	0.00210	0.00129	0.00170	0.00252	0.00231	0.00205
GalCer d18:1/16:0	0.06500	0.05728	0.03990	0.05232	0.03656	0.07189	0.13312	0.08768	0.09690	0.09377	0.12405	0.08675	0.11042	0.08031	0.10850	0.12955	0.15029	0.12120
GalCer d18:1/16:1	0.00017	0.00020	0.00024	0.00021	0.00021	0.00034	0.00067	0.00040	0.00062	0.00060	0.00071	0.00055	0.00075	0.00043	0.00050	0.00082	0.00090	0.00081
GalCer d18:1/18:0	0.44230	0.40830	0.26443	0.34631	0.25711	0.42538	0.44885	0.29099	0.33013	0.30767	0.40720	0.28649	0.36276	0.23208	0.29212	0.35351	0.44202	0.34090
GalCer d18:1/18:1	0.00491	0.00616	0.00607	0.00643	0.00375	0.00412	0.00854	0.00758	0.00863	0.00842	0.00658	0.00508	0.00647	0.00653	0.00770	0.00885	0.00590	0.00523
GalCer d18:1/20:0	0.02028	0.01819	0.01319	0.01621	0.01269	0.01998	0.03939	0.02465	0.02733	0.02539	0.03162	0.02416	0.03090	0.01993	0.02507	0.03217	0.03109	0.02851
GalCer d18:1/20:1	0.00042	0.00039	0.00049	0.00043	0.00022	0.00024	0.00117	0.00084	0.00098	0.00089	0.00101	0.00084	0.00078	0.00077	0.00084	0.00110	0.00093	0.00095
GalCer d18:1/22:0	0.03576	0.03235	0.02533	0.02953	0.02472	0.03500	0.10977	0.06825	0.07608	0.07530	0.09241	0.07194	0.07939	0.05424	0.06690	0.08830	0.08734	0.08026
GalCer d18:1/22:1	0.00191	0.00177	0.00118	0.00131	0.00114	0.00176	0.01209	0.00748	0.00824	0.00813	0.01068	0.00764	0.00700	0.00471	0.00628	0.00848	0.00777	0.00694
GalCer d18:1/24:0	0.02790	0.02255	0.01611	0.02074	0.01621	0.02464	0.06965	0.04045	0.04564	0.04380	0.05521	0.04038	0.07044	0.04260	0.05410	0.07241	0.07309	0.06948
GalCer d18:1/24:1	0.02623	0.02066	0.01320	0.01751	0.01274	0.02326	0.09674	0.05264	0.05939	0.05611	0.07355	0.05400	0.08044	0.04864	0.06290	0.07888	0.08452	0.07335
GalCer d18:1/26:0	0.00191	0.00208	0.00186	0.00203	0.00123	0.00170	0.00317	0.00230	0.00284	0.00260	0.00243	0.00179	0.00258	0.00242	0.00289	0.00307	0.00261	0.00264
GalCer d18:1/26:1	0.00050	0.00041	0.00028	0.00041	0.00025	0.00034	0.00130	0.00081	0.00097	0.00095	0.00114	0.00093	0.00138	0.00093	0.00116	0.00131	0.00146	0.00114
LacCer d18:1/16:0	0.06248	0.04486	0.02970	0.03544	0.02235	0.06132	0.07608	0.03830	0.04443	0.04289	0.05454	0.04348	0.05882	0.02801	0.04653	0.05670	0.08218	0.05676
LacCer d18:1/18:0	0.13474	0.10214	0.05324	0.07650	0.04427	0.11359	0.10434	0.04779	0.04950	0.05467	0.07229	0.05136	0.07388	0.03456	0.04972	0.00222	0.10476	0.06377
LacCer d18:1/20:0	0.00921	0.00510	0.00238	0.00436	0.00268	0.00637	0.01243	0.00676	0.00763	0.00688	0.00656	0.00855	0.00795	0.00353	0.00530	0.00574	0.01012	0.00653
LacCer d18:1/22:0	0.01286	0.00753	0.00442	0.00604	0.00256	0.00833	0.02611	0.01376	0.01233	0.01364	0.01667	0.01435	0.01501	0.00652	0.01169	0.01087	0.02094	0.01332
LacCer d18:1/22:1	0.00220	0.00124	0.00101	0.00126	0.00112	0.00236	0.00558	0.00303	0.00260	0.00253	0.00542	0.00697	0.00325	0.00131	0.00200	0.00301	0.00498	0.00242
LacCer d18:1/24:0	0.00850	0.00409	0.00392	0.00513	0.00266	0.00851	0.02665	0.01152	0.01470	0.01762	0.01697	0.01529	0.02172	0.00919	0.01353	0.01337	0.02818	0.01921
LacCer d18:1/24:1	0.01612	0.00959	0.00536	0.00767	0.00477	0.01268	0.03957	0.01882	0.01877	0.02182	0.03078	0.02319	0.02473	0.01352	0.01599	0.02315	0.03524	0.02661
PC 30:0	19.65318	18.42581	18.01096	19.83852	16.00068	22.53957	16.91211	12.52783	12.16847	16.68752	16.62289	12.30422	15.91423	11.84651	15.08348	16.39606	18.02509	17.20752
PC 32:0	26.70829	24.36462	21.46745	22.56379	19.52500	27.02059	15.76329	14.46393	15.10026	15.54107	18.36383	14.85217	16.09385	15.36206	16.75229	16.02953	17.91921	15.24026
PC 32:1	12.05411	11.35981	12.92399	11.75117	12.22501	14.35371	18.56892	16.59494	15.30673	16.65190	18.48896	19.47778	15.61215	16.71923	17.61408	14.75333	16.85297	17.10418
PC 34:0	7.37749	6.43733	4.86225	5.88305	5.70816	6.32896	3.16618	2.89611	2.72900	2.85831	3.11670	3.18965	3.53889	3.27934	3.74628	4.17802	4.09242	3.54692
PC 34:1	18.39201	19.44021	15.54132	16.99821	14.92485	19.51176	18.92329	17.55026	18.07867	18.84128	18.45054	17.45543	19.67609	17.70267	18.54464	19.61154	21.01967	17.68418
PC 34:2	4.87851	5.06862	4.53061	4.72635	4.88067	4.90707	6.90070	5.60410	6.44735	6.62550	6.22043	6.51308	5.62970	5.50117	6.35795	5.65255	5.54042	6.47311
PC 36:0	0.85794	0.70097	0.51265	0.65381	0.63305	0.73923	0.02659	0.46711	0.45195	0.46029	0.52452	0.47822	0.59825	0.57206	0.64243	0.59755	0.64362	0.57986
PC 36:1	7.45454	6.29749	5.01665	5.91575	5.39408	6.75010	5.13331	4.49333	4.34109	5.00016	5.32479	4.87522	5.99700	6.05381	5.96264	5.88877	7.11639	6.20388
PC 36:2	5.02996	4.52519	3.56905	4.78039	3.92845	5.13480	7.67702	7.24447	6.15567	7.79207	8.00636	7.21895	7.69506	7.55969	7.75408	7.91845	9.01805	8.11928
PC 36:3	3.73583	3.33693	3.19998	3.54804	3.26632	3.85433	4.06323	3.23911	3.03916	3.31570	4.06048	3.07646	3.42305	3.17745	3.35721	3.46357	3.52616	3.74691
PC 36:4	9.72948	9.71531	8.66753	9.18610	8.84039	10.51945	2.93129	2.39515	1.93219	2.06034	2.80132	1.83302	2.88865	2.51414	3.11323	2.74357	3.43175	2.87573
PC 38:1	0.09867	0.07790	0.05252	0.06861	0.06571	0.08285	0.28846	0.22252	0.18268	0.19203	0.28086	0.19063	0.30575	0.24760	0.26024	0.31867	0.33433	0.29509
PC 38:2	0.39223	0.30651	0.23616	0.26118	0.26065	0.31651	1.17001	0.92374	0.80794	0.83209	1.04561	0.86544	1.18388	1.02975	1.11447	1.23924	1.40897	1.21886
PC 38:3	1.63126	1.45071	1.19439	1.37343	1.34258	1.75068	2.73532	1.91084	1.62702	1.80454	2.42112	1.61043	2.65357	2.08503	2.63759	2.71194	2.93734	2.86340
PC 38:4	8.67344	7.50546	5.94828	6.41234	6.08284	8.69393	3.37462	2.56733	2.35352	2.46236	2.98878	2.12700	4.07282	3.35820	3.45587	4.01099	4.97434	3.76008
PC 38:5	5.15909	4.21053	3.81759	4.75476	3.85009	4.71435	1.90630	1.24903	0.96941	1.30866	1.55700	1.04393	2.07584	1.57043	1.83562	1.72250	2.28124	1.80860
PC 38:6	2.32146	2.33422	2.33622	2.29512	2.03624	2.55322	1.43013	0.96987	0.84357	0.88245	1.30277	0.91348	1.29802	1.13380	1.32180	1.33627	1.61788	1.47454
PC 40:4	1.31259	0.93662	0.65485	0.78411	0.73860	0.94256	0.51964	0.31287	0.23211	0.26952	0.38988	0.23200	0.65906	0.37316	0.42149	0.53382	0.64057	0.53017
PC 40:5	2.63733	2.03941	1.53073	1.66457	1.37980	1.99320	0.70382	0.37188	0.34236	0.38325	0.51379	0.33020	0.88826	0.48818	0.64115	0.74747	0.92481	0.75370
PC 40:6	3.30520	2.97379	2.09652	2.73793	1.75922	3.58178	1.03128	0.60350	0.43646	0.52243	0.78073	0.41229	1.24079	0.70993	0.94750	1.18239	1.41779	1.08892
PC 40:7	1.24743	1.08287	0.82522	1.01759	0.65337	1.06102	0.54654	0.24971	0.21525	0.25442	0.36480	0.19629	0.70186	0.36477	0.52611	0.61828	0.86728	0.55119
PC 42:5	0.53441	0.44617	0.22496	0.39214	0.22748	0.44056	0.10207	0.05966	0.04745	0.05196	0.07264	0.04345	0.12793	0.07243	0.08828	0.12205	0.16740	0.10371
PC 42:6	0.44472	0.38071	0.20316	0.31980	0.17766	0.39704	0.07976	0.04606	0.03133	0.03888	0.05093	0.02947	0.11257	0.05259	0.06632	0.09071	0.13417	0.07527
PC 42:7	0.28116	0.25571	0.13197	0.22159	0.11599	0.29255	0.06482	0.03561	0.02962	0.02859	0.04640	0.02261	0.08522	0.04429	0.05501	0.07096	0.09542	0.07345
PCe 30:0	0.33905	0.31892	0.28412	0.34767	0.28778	0.39184	1.15349	0.57403	0.72649	0.87744	0.95468	0.46559	0.64999	0.44127	0.57035	0.71624	0.75900	0.67852
PCe 32:0	1.62574	1.35633	1.22088	1.48083	1.21222	1.61558	4.32233	3.14248	3.20683	3.52968	3.82706	3.02988	2.52896	1.99600	2.61698	2.67837	2.96751	2.59219
PCe 32:1	0.29245	0.27120	0.25466	0.28387	0.27054	0.32313	1.26259	1.00518	1.06170	1.26103	1.22351	1.28709	0.82486	0.81296	0.98113	0.78207	0.92947	0.89713
PCe 34:0	0.58357	0.46511	0.41723	0.43682	0.46958	0.52047	0.69362	0.61538	0.62637	0.64324	0.74880	0.67532	0.58941	0.54616	0.60389	0.65317	0.69155	0.59900
PCe 34:1	1.42630	1.32204	1.11715	1.30006	1.11101	1.44045	2.35057	2.16675	2.03119	2.35224	2.36654	2.29634	1.85131	1.77311	1.95918	2.00924	2.06400	1.90942
PCe 34:2	0.22910	0.19039	0.18301	0.21781	0.17264	0.22300	0.62336	0.50867	0.49538	0.50405	0.59071	0.54954	0.41544	0.39304	0.42315	0.44570	0.44991	0.42023
PCe 36:0	0.07500	0.06147	0.05772	0.06352	0.05979	0.07759	0.10455	0.08810	0.08648	0.09561	0.10940	0.09849	0.09265	0.08701	0.09880	0.10015	0.10482	0.09614
PCe 36:1	0.22812	0.17540	0.15697	0.17094	0.16011	0.22189	0.74208	0.60050	0.63323	0.65484	0.71342	0.68614	0.56246	0.52403	0.58963	0.59664	0.65025	0.61939
PCe 36:2	0.20310	0.12570	0.11040	0.14492	0.12239	0.14094	0.77030	0.65296	0.61863	0.69893	0.76951	0.64077	0.60468	0.49736	0.56021	0.53949	0.59078	0.57304
PCe 36:3	0.12378	0.09015	0.08018	0.09285	0.07673	0.09158	0.33862	0.26091	0.27623	0.29796	0.31583	0.26616	0.21221	0.17897	0.21817	0.21455	0.22920	0.21426
PCe 36:4	0.17896	0.16233	0.14886	0.16767	0.14113	0.19380	0.16473	0.11974	0.09432	0.12968	0.13592	0.09958	0.11737	0.09637	0.11497	0.11085	0.13520	0.10072
PCe 38:0	0.28299	0.29770	0.22728	0.27585	0.22089	0.33771	0.15827	0.10656	0.08683	0.10565	0.13763	0.09159	0.17206	0.12517	0.13615	0.14863	0.19258	0.15241
PCe 38:1	0.05291	0.04932	0.03759	0.04347	0.04225	0.05964	0.08286	0.07188	0.07211	0.07528	0.08042	0.06664	0.08429	0.07558	0.07178	0.08624	0.08960	0.07520
PCe 38:2	0.04482	0.03087	0.02465	0.02831	0.02716	0.03093	0.21092	0.15178	0.14323	0.14470	0.17987	0.14293	0.15880	0.13829	0.15465	0.17566	0.17231	0.16599
PCe 38:3	0.10067	0.06474	0.05818	0.06875	0.05559	0.07660	0.38638	0.29654	0.24308	0.31641	0.37089	0.24865	0.30471	0.23460	0.25578	0.30079	0.31688	0.25759
PCe 38:4	0.32430	0.25223	0.21070	0.25522	0.20381	0.28346	0.35140	0.22675	0.19676	0.23197	0.26845	0.19785	0.26800	0.19598	0.21165	0.27015	0.29612	0.24779
PCe 38:5	0.27673	0.21268	0.16715	0.24827	0.16849	0.29168	0.16438	0.09801	0.08538	0.09083	0.12339	0.08673	0.15864	0.09768	0.11651	0.13634	0.16895	0.12783
PCe 38:6	0.12937	0.09942	0.08634	0.11140	0.07384	0.11550	0.08167	0.04777	0.04467	0.04779	0.06114	0.04115	0.07577	0.04471	0.05991	0.06569	0.08681	0.06416
PCe 40:4	0.15240	0.10694	0.07346	0.08955	0.07030	0.11777	0.10561	0.06321	0.05566	0.05761	0.08022	0.05010	0.09113	0.06030	0.07211	0.08924	0.10490	0.08868
PCe 40:5	0.25773	0.17989	0.12573	0.17325	0.10059	0.19246	0.07951	0.04535	0.03826	0.04111	0.05747	0.03704	0.07980	0.04665	0.05770	0.07679	0.09173	0.06693
PCe 40:6	0.21522	0.14639	0.10942	0.14534	0.09275	0.16223	0.09097	0.05298	0.04128	0.04508	0.06759	0.04071	0.08426	0.04956	0.06861	0.07409	0.09322	0.07594
PCe 40:7	0.16433	0.11432	0.07735	0.12270	0.06480	0.13541	0.07996	0.04459	0.03362	0.03960	0.05246	0.03317	0.08489	0.05078	0.05696	0.07350	0.10329	0.07321
PCe 42:5	0.04070	0.02944	0.01707	0.02332	0.01645	0.03342	0.01748	0.00772	0.00712	0.00796	0.01148	0.00754	0.01611	0.00994	0.01104	0.01697	0.01604	0.01445
PCe 42:6	0.04370	0.03354	0.01895	0.02935	0.01793	0.03635	0.01903	0.00936	0.00854	0.00880	0.01334	0.00703	0.01897	0.01181	0.01383	0.01689	0.01992	0.01488
LPC 16:0	0.49682	0.44950	0.25068	0.27061	0.27336	0.34082	0.47837	0.20584	0.24336	0.27744	0.30428	0.14076	0.54551	0.24457	0.34781	0.34294	0.46549	0.31160
LPC 16:1	0.03011	0.03247	0.01454	0.01634	0.01753	0.02419	0.06668	0.03428	0.03837	0.04177	0.05365	0.02480	0.04465	0.02676	0.04049	0.03875	0.06655	0.04035
LPC 18:0	0.18942	0.19819	0.14814	0.13081	0.11904	0.13795	0.13329	0.05864	0.07260	0.07920	0.07833	0.03699	0.21109	0.09331	0.14585	0.12281	0.21747	0.13802
LPC 18:1	0.09713	0.10346	0.05893	0.06141	0.05607	0.08311	0.17343	0.08584	0.10100	0.10837	0.11582	0.06324	0.19299	0.09876	0.14929	0.14543	0.24084	0.14993
LPC 20:0	0.00137	0.00191	0.00139	0.00121	0.00130	0.00149	0.00278	0.00110	0.00150	0.00198	0.00185	0.00100	0.00292	0.00165	0.00345	0.00231	0.00553	0.00339
LPC 20:1	0.00326	0.00418	0.00245	0.00239	0.00220	0.00309	0.00806	0.00394	0.00485	0.00613	0.00612	0.00309	0.01077	0.00576	0.00936	0.00853	0.01493	0.00880
LPC 20:2	0.00241	0.00293	0.00177	0.00176	0.00181	0.00225	0.00431	0.00236	0.00262	0.00290	0.00319	0.00166	0.00425	0.00262	0.00394	0.00388	0.00672	0.00458
LPC 20:3	0.00596	0.00679	0.00332	0.00371	0.00376	0.00565	0.00797	0.00406	0.00500	0.00547	0.00587	0.00295	0.00725	0.00502	0.00681	0.00670	0.01120	0.00734
LPC 20:4	0.02461	0.02923	0.01281	0.01433	0.01539	0.02476	0.00637	0.00368	0.00451	0.00451	0.00543	0.00278	0.00664	0.00413	0.00633	0.00604	0.01011	0.00607
LPCe 16:0	0.00278	0.00243	0.00134	0.00146	0.00159	0.00205	0.00986	0.00486	0.00493	0.00650	0.00699	0.00370	0.00558	0.00327	0.00518	0.00476	0.00715	0.00488
LPCe 18:0	0.00692	0.00667	0.00410	0.00402	0.00362	0.00501	0.02031	0.00865	0.01011	0.01316	0.01348	0.00592	0.01597	0.00864	0.01280	0.01293	0.01808	0.01233
LPCe 18:1	0.00079	0.00083	0.00041	0.00043	0.00060	0.00089	0.00533	0.00256	0.00308	0.00316	0.00372	0.00175	0.00309	0.00192	0.00277	0.00254	0.00419	0.00294
LPCe 20:0	0.00061	0.00069	0.00063	0.00059	0.00056	0.00055	0.00148	0.00074	0.00079	0.00093	0.00096	0.00066	0.00117	0.00075	0.00123	0.00109	0.00144	0.00117
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
Cer d18:1/16:0
Cer d18:1/16:1
Cer d18:1/18:0
Cer d18:1/18:1
Cer d18:1/20:0
Cer d18:1/20:1
Cer d18:1/22:0
Cer d18:1/22:1
Cer d18:1/24:0
Cer d18:1/24:1
SM d18:1/16:0
SM d18:1/16:1
SM d18:1/18:0
SM d18:1/18:1
SM d18:1/20:0
SM d18:1/20:1
SM d18:1/22:0
SM d18:1/22:1
SM d18:1/24:0
SM d18:1/24:1
SM d18:1/26:0
SM d18:1/26:1
dhSM d18:0/16:0
dhSM d18:0/18:0
dhSM d18:0/18:1
dhSM d18:0/22:0
dhSM d18:0/24:0
dhSM d18:0/24:1
GalCer d18:0/16:0
GalCer d18:0/16:1
GalCer d18:0/18:0
GalCer d18:0/18:1
GalCer d18:0/20:0
GalCer d18:0/22:0
GalCer d18:0/24:0
GalCer d18:0/24:1
GalCer d18:1/16:0
GalCer d18:1/16:1
GalCer d18:1/18:0
GalCer d18:1/18:1
GalCer d18:1/20:0
GalCer d18:1/20:1
GalCer d18:1/22:0
GalCer d18:1/22:1
GalCer d18:1/24:0
GalCer d18:1/24:1
GalCer d18:1/26:0
GalCer d18:1/26:1
LacCer d18:1/16:0
LacCer d18:1/18:0
LacCer d18:1/20:0
LacCer d18:1/22:0
LacCer d18:1/22:1
LacCer d18:1/24:0
LacCer d18:1/24:1
PC 30:0
PC 32:0
PC 32:1
PC 34:0
PC 34:1
PC 34:2
PC 36:0
PC 36:1
PC 36:2
PC 36:3
PC 36:4
PC 38:1
PC 38:2
PC 38:3
PC 38:4
PC 38:5
PC 38:6
PC 40:4
PC 40:5
PC 40:6
PC 40:7
PC 42:5
PC 42:6
PC 42:7
PCe 30:0
PCe 32:0
PCe 32:1
PCe 34:0
PCe 34:1
PCe 34:2
PCe 36:0
PCe 36:1
PCe 36:2
PCe 36:3
PCe 36:4
PCe 38:0
PCe 38:1
PCe 38:2
PCe 38:3
PCe 38:4
PCe 38:5
PCe 38:6
PCe 40:4
PCe 40:5
PCe 40:6
PCe 40:7
PCe 42:5
PCe 42:6
LPC 16:0
LPC 16:1
LPC 18:0
LPC 18:1
LPC 20:0
LPC 20:1
LPC 20:2
LPC 20:3
LPC 20:4
LPCe 16:0
LPCe 18:0
LPCe 18:1
LPCe 20:0
METABOLITES_END
#END