#METABOLOMICS WORKBENCH tnuriel_20210602_131536_mwtab.txt DATATRACK_ID:2670 STUDY_ID:ST001820 ANALYSIS_ID:AN002956 PROJECT_ID:000000
VERSION             	1
CREATED_ON             	June 7, 2021, 12:36 pm
#PROJECT
PR:PROJECT_TYPE                  	Lipidomics analysis
PR:PROJECT_SUMMARY               	Apolipoprotein E ε4 (APOE4) is the primary genetic risk factor for the
PR:PROJECT_SUMMARY               	late-onset form of Alzheimer's disease (AD). Although the reason for this
PR:PROJECT_SUMMARY               	association is not completely understood, researchers have uncovered numerous
PR:PROJECT_SUMMARY               	effects of APOE4 expression on AD-relevant brain processes, including amyloid
PR:PROJECT_SUMMARY               	beta (Aβ) accumulation, lipid metabolism, endosomal-lysosomal processing and
PR:PROJECT_SUMMARY               	bioenergetics. In this study, we aimed to determine the effect of APOE4 allelic
PR:PROJECT_SUMMARY               	dosage on regional brain lipid composition in aged mice, as well as in cultured
PR:PROJECT_SUMMARY               	neurons. We performed a targeted lipidomic analysis on the entorhinal cortex
PR:PROJECT_SUMMARY               	(EC) and primary visual cortex (PVC) from 14–15 month-old APOE3/3, APOE3/4,
PR:PROJECT_SUMMARY               	and APOE4/4 targeted replacement mice, as well as on WT neurons cultured with
PR:PROJECT_SUMMARY               	conditioned media from APOE3/3 or APOE4/4 astrocytes. Our results reveal that
PR:PROJECT_SUMMARY               	the EC possesses increased susceptibility to APOE4-associated lipid alterations
PR:PROJECT_SUMMARY               	compared to the PVC. In the EC, APOE4 expression showed a dominant effect in
PR:PROJECT_SUMMARY               	decreasing diacylglycerol (DAG) levels, and a semi-dominant additive effect in
PR:PROJECT_SUMMARY               	the upregulation of multiple ceramide, glycosylated sphingolipid and
PR:PROJECT_SUMMARY               	bis(monoacylglycerol)phosphate (BMP) species, lipids known to accumulate as a
PR:PROJECT_SUMMARY               	result of endosomal-lysosomal dysfunction and defective lysosomal clearance.
PR:PROJECT_SUMMARY               	Neurons treated with conditioned media from APOE4 vs. APOE3 astrocytes also
PR:PROJECT_SUMMARY               	showed similar alterations of DAG and BMP species as those observed in the mouse
PR:PROJECT_SUMMARY               	EC. Our results suggest that APOE4 expression differentially modulates regional
PR:PROJECT_SUMMARY               	and neuronal lipid signatures, which may underlie the increased susceptibility
PR:PROJECT_SUMMARY               	of EC-localized neurons to AD pathology.
PR:INSTITUTE                     	Columbia University
PR:FIRST_NAME                    	Tal
PR:ADDRESS                       	630 W 168th St., P&S 12-430
PR:EMAIL                         	tn2283@cumc.columbia.edu
PR:PHONE                         	2123045683
PR:PROJECT_TITLE                 	APOE4-associated differences in lipidomics signatures in mouse brain and
PR:PROJECT_TITLE                 	cultured neurons
PR:LAST_NAME                     	Nuriel
#STUDY
ST:STUDY_TITLE                   	WT neurons treated with APOE3/3 and APOE4/4 ACM
ST:STUDY_SUMMARY                 	We performed a targeted lipidomic analysis on WT neurons treated with astrocyte
ST:STUDY_SUMMARY                 	conditioned media (ACM) from APOE3/3 or APOE4/4 astrocytes.
ST:INSTITUTE                     	Columbia University
ST:LAST_NAME                     	Nuriel
ST:FIRST_NAME                    	Tal
ST:ADDRESS                       	630 W 168th St., P&S 12-430
ST:EMAIL                         	tn2283@cumc.columbia.edu
ST:PHONE                         	2123045683
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENDER                        	Not applicable
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	1	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	2	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	3	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	4	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	5	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	6	Condition:No ACM	
SUBJECT_SAMPLE_FACTORS           	-	7	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	8	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	9	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	10	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	11	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	12	Condition:APOE3/3 ACM	
SUBJECT_SAMPLE_FACTORS           	-	13	Condition:APOE4/4 ACM	
SUBJECT_SAMPLE_FACTORS           	-	14	Condition:APOE4/4 ACM	
SUBJECT_SAMPLE_FACTORS           	-	15	Condition:APOE4/4 ACM	
SUBJECT_SAMPLE_FACTORS           	-	16	Condition:APOE4/4 ACM	
SUBJECT_SAMPLE_FACTORS           	-	17	Condition:APOE4/4 ACM	
SUBJECT_SAMPLE_FACTORS           	-	18	Condition:APOE4/4 ACM	
#COLLECTION
CO:COLLECTION_SUMMARY            	Astrocyte conditioned media (ACM) was obtained from immortalized astrocyte cell
CO:COLLECTION_SUMMARY            	lines (a gift from Dr. David Holtzman) that were originally generated from
CO:COLLECTION_SUMMARY            	primary astrocytes from P1-2 pups of APOE targeted replacement mice (42). The
CO:COLLECTION_SUMMARY            	immortalized astrocytes were conditioned with Neurobasal media supplemented with
CO:COLLECTION_SUMMARY            	B27, Glutamax-I, Normocin and 1% penicillin/streptomycin for 24 hours. This ACM
CO:COLLECTION_SUMMARY            	was then collected, stored at -80ºC, and thawed prior to use. WT primary
CO:COLLECTION_SUMMARY            	cortical neuronal cultures were obtained from embryonic day 17 (E17) C57Bl/6
CO:COLLECTION_SUMMARY            	embryos. Briefly, pregnant mice were euthanized by cervical dislocation and the
CO:COLLECTION_SUMMARY            	embryo brains extracted. The meninges were carefully stripped off and the
CO:COLLECTION_SUMMARY            	cortices dissected out. The cortices were then enzymatically dissociated in
CO:COLLECTION_SUMMARY            	0.25% Trypsin-EDTA and resuspended in Neurobasal media supplemented with B27,
CO:COLLECTION_SUMMARY            	Glutamax-I, Normocin and 1% penicillin/streptomycin. Dissociated cells were
CO:COLLECTION_SUMMARY            	counted and 300K neurons per well were plated directly into ACM in poly-D-lysine
CO:COLLECTION_SUMMARY            	(PDL)-coated 6-well plates. ACM-treated neurons were then incubated at 37⁰C
CO:COLLECTION_SUMMARY            	for 7 days in a humidified chamber with 5% CO2, with 50% media exchange (with
CO:COLLECTION_SUMMARY            	newly thawed ACM) once every 3 days.
CO:SAMPLE_TYPE                   	Neurons
#TREATMENT
TR:TREATMENT_SUMMARY             	WT neurons were incubated for 7 days in either no ACM, APOE3/3 ACM, or APOE4/4
TR:TREATMENT_SUMMARY             	ACM.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Lipid and small-molecule metabolite extraction was performed using a methyl
SP:SAMPLEPREP_SUMMARY            	tert-butyl ether (MTBE)/methanol extraction protocol. Briefly, harvested neurons
SP:SAMPLEPREP_SUMMARY            	were homogenized in ice-cold methanol. Following homogenization, samples were
SP:SAMPLEPREP_SUMMARY            	incubated in 1200 ul of MTBE for 1 hr at room temperature to separate
SP:SAMPLEPREP_SUMMARY            	organic-soluble lipids from aqueous-soluble lipids and other small-molecules.
SP:SAMPLEPREP_SUMMARY            	Finally, 360 ul of ultrapure water was added (for a final ratio of 3:1:0.9
SP:SAMPLEPREP_SUMMARY            	MTBE:methanol:water) to resolve the two liquid phases, and each samples were
SP:SAMPLEPREP_SUMMARY            	centrifuged at 10,000 x g for 10 min. For this experiment, the upper organic
SP:SAMPLEPREP_SUMMARY            	phase was collected from each sample and stored in a separate tube, and the
SP:SAMPLEPREP_SUMMARY            	remaining protein pellets were resuspended in 25 mM ammonium bicarbonate, pH 8,
SP:SAMPLEPREP_SUMMARY            	with 2.5% SDS. A BCA protein assay was performed on each protein fraction, and
SP:SAMPLEPREP_SUMMARY            	the organic phase was normalized to their protein concentration equivalent with
SP:SAMPLEPREP_SUMMARY            	100% methanol. All samples were then stored at -80°C prior to analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Normal phase
CH:CHROMATOGRAPHY_TYPE           	Normal phase
CH:INSTRUMENT_NAME               	Agilent 1260
CH:COLUMN_NAME                   	Phenomenex Luna NH2 (150 x 2.1mm, 3um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6490 QQQ
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Lipid profiling was performed using an Agilent 1260 HPLC coupled to an Agilent
MS:MS_COMMENTS                   	6490 triple quadrupole (QQQ) mass spectrometer (41). Each sample was run through
MS:MS_COMMENTS                   	three separate chromatographic conditions (reverse-phase negative mode,
MS:MS_COMMENTS                   	reverse-phase positive mode and normal-phase positive mode) for the effective
MS:MS_COMMENTS                   	quantification of over 337 distinct lipids from over 28 lipid subclasses, as
MS:MS_COMMENTS                   	previously described (41). Individual lipid species were measured by multiple
MS:MS_COMMENTS                   	reaction monitoring transitions.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	area under the curve
MS_METABOLITE_DATA_START
Samples	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Factors	Condition:No ACM	Condition:No ACM	Condition:No ACM	Condition:No ACM	Condition:No ACM	Condition:No ACM	Condition:APOE3/3 ACM	Condition:APOE3/3 ACM	Condition:APOE3/3 ACM	Condition:APOE3/3 ACM	Condition:APOE3/3 ACM	Condition:APOE3/3 ACM	Condition:APOE4/4 ACM	Condition:APOE4/4 ACM	Condition:APOE4/4 ACM	Condition:APOE4/4 ACM	Condition:APOE4/4 ACM	Condition:APOE4/4 ACM
FC b	122.07	142.36284	88.74605	111.54929	87.31083	202.41588	86.87652	55.46933	69.37448	72.40067	81.66512	62.72413	100.98070	89.09218	110.80087	137.60644	149.61587	111.71074
CE 16:0	0.07953	0.06443	0.03934	0.04735	0.03527	0.05904	0.05595	0.02561	0.02223	0.02779	0.03998	0.02507	0.06084	0.03152	0.03677	0.04085	0.06163	0.04641
CE 16:1	0.03495	0.02841	0.02022	0.02047	0.01606	0.02389	0.03539	0.01990	0.01679	0.01888	0.02709	0.02023	0.06831	0.02850	0.02746	0.03328	0.05990	0.03616
CE 18:0	0.02772	0.02821	0.01660	0.02206	0.01612	0.02424	0.01565	0.01088	0.00861	0.01136	0.01242	0.00973	0.02261	0.01057	0.01345	0.01572	0.02093	0.01722
CE 18:1	0.16454	0.13957	0.07833	0.09775	0.07337	0.11067	0.11699	0.06030	0.05828	0.06242	0.07472	0.06428	0.13755	0.06961	0.08155	0.08105	0.12415	0.09883
CE 18:2	0.05598	0.04294	0.02008	0.02653	0.01744	0.03219	0.04996	0.02001	0.02105	0.02405	0.03197	0.01922	0.04872	0.02435	0.02285	0.02761	0.04695	0.03463
CE 20:1	0.00259	0.00200	0.00204	0.00164	0.00095	0.00168	0.00315	0.00240	0.00155	0.00261	0.00194	0.00218	0.00631	0.00293	0.00365	0.00386	0.00535	0.00443
CE 20:2	0.00318	0.00295	0.00193	0.00221	0.00217	0.00371	0.00289	0.00144	0.00117	0.00159	0.00214	0.00131	0.00606	0.00210	0.00414	0.00461	0.00451	0.00423
CE 20:3	0.01173	0.01181	0.00671	0.00885	0.00564	0.01025	0.00846	0.00497	0.00369	0.00468	0.00623	0.00343	0.01151	0.00503	0.00694	0.00882	0.01230	0.00949
CE 20:4	0.03229	0.03387	0.02248	0.02812	0.01656	0.02931	0.02872	0.01252	0.01368	0.01502	0.02060	0.01415	0.02955	0.01638	0.01628	0.02123	0.03358	0.02664
CE 22:3	0.00148	0.00122	0.00101	0.00149	0.00107	0.00131	0.00099	0.00039	0.00056	0.00077	0.00053	0.00056	0.00246	0.00087	0.00089	0.00122	0.00185	0.00137
CE 22:4	0.00763	0.00697	0.00512	0.00713	0.00421	0.00788	0.00140	0.00106	0.00103	0.00141	0.00136	0.00086	0.00241	0.00167	0.00172	0.00195	0.00223	0.00261
CE 22:6	0.01427	0.01791	0.01245	0.01196	0.00731	0.01647	0.00840	0.00593	0.00434	0.00536	0.00552	0.00615	0.01203	0.00580	0.00540	0.01021	0.01415	0.01072
CE 24:6	0.00315	0.00251	0.00201	0.00243	0.00160	0.00265	0.00042	0.00019	0.00013	0.00034	0.00033	0.00040	0.00037	0.00026	0.00041	0.00041	0.00059	0.00039
AC C12:0	0.00820	0.00638	0.01465	0.00662	0.00364	0.00667	0.01487	0.00626	0.00649	0.00824	0.01198	0.00611	0.01795	0.00709	0.00942	0.00963	0.01145	0.01107
AC C14:0	0.00686	0.00652	0.00446	0.00717	0.00332	0.00916	0.02587	0.01228	0.01320	0.01559	0.02132	0.01258	0.04878	0.01761	0.02305	0.02355	0.03196	0.02474
AC C16:0	0.01228	0.01460	0.01151	0.01478	0.01064	0.02673	0.05331	0.02635	0.03279	0.04765	0.05132	0.03087	0.15749	0.04940	0.06881	0.05997	0.07789	0.05866
AC C18:0	0.01357	0.01240	0.01244	0.01374	0.00728	0.01586	0.01754	0.00896	0.00896	0.01645	0.01370	0.00927	0.07279	0.02202	0.02803	0.02555	0.03150	0.02426
AC C18:1	0.03142	0.04861	0.12424	0.12944	0.06213	0.15041	0.08696	0.08520	0.07775	0.08932	0.11508	0.15260	0.19511	0.11790	0.13365	0.11469	0.13726	0.20135
AC C2:0	0.06380	0.13159	0.00200	0.00298	0.08173	0.00459	0.58089	0.29849	0.18444	0.41435	0.33797	0.00298	0.05385	0.08113	0.09929	0.05030	0.19560	0.00233
AC C3:0	0.06207	0.13362	0.00186	0.00347	0.09127	0.00347	0.54147	0.25071	0.17302	0.42853	0.32340	0.00177	0.06764	0.09777	0.12444	0.06311	0.24095	0.00361
AC C6:0	0.00318	0.00352	0.00194	0.00257	0.00279	0.00235	0.00761	0.00339	0.00423	0.00654	0.00481	0.00208	0.00759	0.00378	0.00451	0.00315	0.00534	0.00340
AC C8:0	0.01693	0.01469	0.01224	0.01536	0.00735	0.01074	0.01339	0.00634	0.00615	0.00732	0.01206	0.00757	0.06715	0.02158	0.01737	0.01541	0.01840	0.01605
MG 16:0	0.23962	0.31475	0.46414	0.09202	0.08208	0.14137	0.10364	0.06639	0.07110	0.05610	0.07257	0.06167	0.34325	0.10571	0.13148	0.14988	0.14854	0.10562
MG 16:1	0.12888	0.07802	0.06622	0.11505	0.05563	0.10703	0.06025	0.04219	0.05546	0.05504	0.12314	0.06063	0.19717	0.08743	0.08158	0.20014	0.07723	0.24152
MG 18:0	0.60412	0.59435	0.87879	0.30564	0.21935	0.37853	0.22470	0.11192	0.13524	0.13631	0.17405	0.13176	0.40143	0.18348	0.22732	0.31048	0.29793	0.23003
MG 18:1	0.09983	0.04876	0.05450	0.09071	0.03231	0.08668	0.04332	0.02295	0.03646	0.04334	0.12785	0.05550	0.17264	0.07281	0.07196	0.19885	0.07910	0.23715
MG 18:2	0.11362	0.04629	0.04713	0.08943	0.03418	0.08467	0.04178	0.02497	0.02904	0.04148	0.11444	0.04268	0.13905	0.05657	0.05851	0.19728	0.05911	0.23432
MG 18:3	0.07913	0.04661	0.04884	0.05636	0.02450	0.09911	0.03360	0.02031	0.02573	0.02721	0.06064	0.03926	0.11644	0.04195	0.05279	0.11095	0.05747	0.10267
DAG 28:0/14:0	0.01505	0.01067	0.01392	0.01173	0.00856	0.01473	0.02946	0.03275	0.03716	0.04081	0.04452	0.04627	0.04791	0.02684	0.03671	0.04983	0.03476	0.02477
DAG 30:0/14:0	0.06657	0.03967	0.05295	0.04586	0.02883	0.05480	0.08232	0.08175	0.09643	0.11734	0.11361	0.11739	0.11962	0.08063	0.11674	0.13022	0.08128	0.07262
DAG 30:1/14:0	0.00450	0.00343	0.00259	0.00405	0.00262	0.00574	0.03217	0.03078	0.04459	0.04277	0.04351	0.04498	0.03713	0.01949	0.02783	0.03163	0.02541	0.01881
DAG 32:0/16:0	0.28910	0.17094	0.22510	0.19179	0.13753	0.24489	0.33906	0.32376	0.47392	0.50484	0.50213	0.53046	0.48997	0.31935	0.45573	0.55759	0.31525	0.28609
DAG 32:1/16:0	0.04994	0.02861	0.02836	0.04272	0.02545	0.05154	0.13085	0.14152	0.18580	0.22746	0.20120	0.21587	0.15592	0.09030	0.13937	0.17075	0.10326	0.09365
DAG 32:2/16:1	0.00875	0.00592	0.00475	0.00646	0.00465	0.01088	0.08337	0.08267	0.10645	0.12340	0.12869	0.13848	0.10330	0.05186	0.06718	0.07973	0.05411	0.04691
DAG 34:0/16:0	0.16079	0.16181	0.29067	0.09324	0.06143	0.12895	0.08483	0.06834	0.08797	0.11987	0.11093	0.10548	0.16309	0.10288	0.15826	0.20505	0.13776	0.09714
DAG 34:1/16:0	0.18855	0.02173	0.07574	0.11743	0.08176	0.15667	0.26460	0.29385	0.38004	0.41130	0.40853	0.44341	0.48175	0.27061	0.41204	0.51323	0.33282	0.27312
DAG 34:2/16:0	0.02619	0.01348	0.01264	0.01603	0.01300	0.02205	0.01625	0.01876	0.02888	0.02888	0.02705	0.03667	0.03054	0.01745	0.02545	0.02811	0.01833	0.01500
DAG 34:2/16:1	0.03964	0.01691	0.01673	0.01954	0.01559	0.02539	0.10628	0.10713	0.13848	0.15593	0.14661	0.17640	0.13123	0.09018	0.11743	0.14071	0.09784	0.08077
DAG 36:0/18:0	0.25770	0.44737	0.73156	0.16377	0.09977	0.21900	0.18165	0.10152	0.09321	0.13285	0.12788	0.09245	0.19608	0.12595	0.15325	0.28121	0.24228	0.13104
DAG 36:1/18:0	0.11664	0.06175	0.04336	0.07548	0.05237	0.09628	0.06362	0.05513	0.07637	0.09528	0.08593	0.08645	0.21168	0.09548	0.15498	0.15407	0.12238	0.09968
DAG 36:2/18:0	0.00807	0.00543	0.00242	0.00375	0.00428	0.00481	0.00595	0.00722	0.00820	0.00989	0.01191	0.01293	0.01520	0.01007	0.01235	0.01468	0.00977	0.00716
DAG 36:2/18:1	0.04320	0.02436	0.02151	0.00421	0.02410	0.03859	0.11095	0.13113	0.16769	0.18070	0.20575	0.19896	0.24734	0.15681	0.18800	0.24098	0.14183	0.12051
DAG 36:3/18:1	0.00851	0.00408	0.00348	0.00349	0.00256	0.00245	0.00656	0.00964	0.01284	0.01069	0.01202	0.01575	0.02207	0.01100	0.01539	0.01565	0.01094	0.00776
DAG 36:4/18:0	0.00035	0.00011	0.00014	0.00367	0.00009	0.00035	0.00010	0.00007	0.00014	0.00019	0.00015	0.00009	0.00240	0.00022	0.00023	0.00033	0.00019	0.00935
DAG 38:1/18:0	0.00074	0.00164	0.00021	0.00221	0.00128	0.00037	0.00052	0.00220	0.00064	0.00327	0.00469	0.00305	0.00785	0.00411	0.00723	0.00569	0.00107	0.00497
DAG 38:2/18:0	0.00033	0.00124	0.00013	0.00121	0.00065	0.00256	0.01106	0.01580	0.01309	0.01114	0.01504	0.01406	0.02361	0.01813	0.02212	0.01400	0.02203	0.01130
DAG 38:2/18:1	0.00200	0.00031	0.00085	0.00006	0.00032	0.00020	0.00503	0.00455	0.00574	0.00753	0.00136	0.00872	0.01392	0.00137	0.01031	0.00066	0.00116	0.00084
DAG 38:3/18:0	0.07006	0.03275	0.02274	0.03684	0.02400	0.03644	0.09018	0.10050	0.13440	0.11893	0.15248	0.15568	0.20898	0.11552	0.14885	0.17646	0.13861	0.08825
DAG 38:3/18:1	0.00117	0.00059	0.00027	0.00006	0.00012	0.00043	0.00195	0.00944	0.01204	0.00556	0.00042	0.01376	0.01039	0.00794	0.00937	0.01341	0.00023	0.00406
DAG 38:4/18:0	0.25889	0.11040	0.05478	0.09065	0.06022	0.09204	0.06545	0.06882	0.09586	0.08598	0.10277	0.10623	0.16615	0.09851	0.12622	0.16594	0.10091	0.07323
DAG 38:4/18:1	0.00307	0.00263	0.00266	0.00233	0.00145	0.00187	0.02840	0.04202	0.05101	0.05211	0.05725	0.06518	0.06441	0.03167	0.04141	0.05213	0.03188	0.02677
DAG 40:4/18:0	0.05126	0.02365	0.01728	0.03162	0.02003	0.04032	0.01206	0.01020	0.01658	0.01862	0.00092	0.01774	0.02938	0.01535	0.02472	0.00153	0.02136	0.01852
DAG 40:4/18:1	0.00094	0.00025	0.00016	0.00006	0.00004	0.00023	0.00295	0.00275	0.00300	0.00485	0.00385	0.00471	0.00653	0.00022	0.00208	0.00554	0.00337	0.00237
DAG 40:5/18:0	0.00792	0.00455	0.00429	0.00400	0.00506	0.00719	0.00427	0.00335	0.00408	0.00520	0.00625	0.00536	0.00819	0.00478	0.00035	0.00785	0.00566	0.00482
DAG 40:5/18:1	0.00038	0.00019	0.00124	0.00304	0.00156	0.00036	0.00210	0.00009	0.00015	0.00256	0.00019	0.00019	0.00042	0.00168	0.00364	0.00365	0.00053	0.00016
DAG 40:6/18:0	0.00385	0.02194	0.00127	0.02893	0.01935	0.03618	0.02238	0.02858	0.02670	0.03817	0.03626	0.03709	0.05387	0.03483	0.05003	0.05994	0.03684	0.03508
DAG 40:6/18:1	0.00103	0.00046	0.00016	0.00066	0.00071	0.00052	0.00062	0.00044	0.00021	0.00122	0.00207	0.00076	0.00163	0.00117	0.00117	0.00036	0.00106	0.00056
TAG 48:0/16:0	0.10780	0.24968	0.33913	0.06257	0.04059	0.07228	0.10162	0.05738	0.05110	0.05909	0.08495	0.04596	0.10679	0.05240	0.05901	0.09543	0.11804	0.07076
TAG 48:1/16:0	0.04803	0.03998	0.02343	0.03357	0.02144	0.03439	0.11352	0.05864	0.05365	0.06291	0.08580	0.04504	0.09651	0.04515	0.05147	0.06722	0.11019	0.06522
TAG 50:0/16:0	0.07061	0.45341	0.76522	0.04918	0.03436	0.05063	0.04447	0.03044	0.02604	0.02819	0.04125	0.02441	0.06645	0.03463	0.04038	0.08573	0.07128	0.04779
TAG 50:1/16:1	0.01405	0.01045	0.00668	0.00873	0.00618	0.00931	0.01686	0.00896	0.00980	0.00964	0.01338	0.00713	0.01775	0.00968	0.01026	0.01415	0.02247	0.01517
TAG 50:2/16:1	0.02925	0.02481	0.01381	0.01849	0.01165	0.01977	0.08949	0.04457	0.03813	0.05124	0.06650	0.03398	0.07719	0.03753	0.04331	0.05562	0.09748	0.05395
TAG 50:3/16:1	0.02229	0.01841	0.01029	0.01422	0.00911	0.01400	0.07820	0.03643	0.03546	0.04200	0.05690	0.02694	0.06328	0.02861	0.03568	0.04327	0.08054	0.03771
TAG 52:0/18:0	0.03991	0.81706	1.53309	0.02184	0.01674	0.02978	0.01544	0.01001	0.00838	0.00882	0.01228	0.00702	0.02627	0.01383	0.01706	0.09248	0.03453	0.01980
TAG 52:1/18:0	0.04936	0.05565	0.03711	0.04083	0.02219	0.04072	0.05050	0.03339	0.02965	0.02924	0.04361	0.02572	0.06394	0.03617	0.04592	0.05604	0.07507	0.05448
TAG 52:2/18:0	0.01472	0.01214	0.00740	0.00974	0.00473	0.00831	0.01281	0.00541	0.00609	0.00617	0.00796	0.00434	0.01167	0.00674	0.00848	0.01043	0.01619	0.00849
TAG 52:3/18:1	0.02859	0.02392	0.01327	0.01810	0.01087	0.01743	0.06267	0.03297	0.02957	0.03494	0.04367	0.02473	0.05863	0.02937	0.03499	0.04463	0.07569	0.04103
TAG 52:4/18:1	0.00609	0.00558	0.00278	0.00343	0.00191	0.00347	0.00617	0.00314	0.00282	0.00318	0.00522	0.00253	0.00581	0.00274	0.00284	0.00457	0.00727	0.00363
TAG 52:5/20:4	0.00557	0.00542	0.00266	0.00402	0.00199	0.00443	0.00163	0.00074	0.00070	0.00073	0.00112	0.00080	0.00207	0.00089	0.00092	0.00178	0.00239	0.00132
TAG 54:0/18:0	0.02010	0.48081	0.91530	0.00767	0.00521	0.01147	0.00396	0.00291	0.00181	0.00201	0.00315	0.00168	0.00727	0.00343	0.00390	0.05502	0.00879	0.00447
TAG 54:1/18:0	0.00605	0.00836	0.00948	0.00342	0.00196	0.00421	0.00487	0.00238	0.00257	0.00281	0.00360	0.00193	0.00615	0.00446	0.00469	0.00547	0.00880	0.00552
TAG 54:2/18:0	0.00743	0.00685	0.00585	0.00499	0.00364	0.00498	0.00858	0.00536	0.00519	0.00558	0.00692	0.00364	0.01226	0.00781	0.00842	0.01015	0.01442	0.00912
TAG 54:4/18:1	0.00862	0.00807	0.00448	0.00576	0.00346	0.00598	0.01260	0.00607	0.00513	0.00684	0.00920	0.00438	0.01618	0.00913	0.00873	0.00994	0.01596	0.01040
TAG 54:4/20:4	0.01276	0.01140	0.00623	0.00874	0.00487	0.00987	0.00287	0.00125	0.00144	0.00160	0.00237	0.00101	0.00416	0.00239	0.00254	0.00359	0.00504	0.00296
TAG 54:5/20:4	0.01223	0.01308	0.00677	0.01012	0.00478	0.01035	0.00373	0.00198	0.00188	0.00210	0.00303	0.00157	0.00548	0.00240	0.00313	0.00385	0.00639	0.00369
TAG 54:6/20:4	0.00489	0.00466	0.00240	0.00401	0.00198	0.00378	0.00127	0.00067	0.00068	0.00055	0.00055	0.00042	0.00114	0.00069	0.00076	0.00085	0.00168	0.00113
TAG 56:3/18:1	0.00197	0.00165	0.00107	0.00088	0.00075	0.00124	0.00562	0.00365	0.00328	0.00368	0.00480	0.00306	0.00690	0.00438	0.00515	0.00610	0.00949	0.00558
TAG 56:4/20:4	0.00422	0.00483	0.00284	0.00449	0.00234	0.00425	0.00159	0.00120	0.00109	0.00109	0.00175	0.00099	0.00363	0.00192	0.00267	0.00313	0.00397	0.00298
TAG 56:5/20:4	0.00506	0.00472	0.00271	0.00412	0.00210	0.00422	0.00314	0.00166	0.00147	0.00177	0.00237	0.00152	0.00500	0.00252	0.00304	0.00429	0.00741	0.00440
TAG 56:6/20:4	0.00279	0.00316	0.00154	0.00200	0.00104	0.00247	0.00117	0.00090	0.00073	0.00089	0.00118	0.00053	0.00219	0.00114	0.00154	0.00241	0.00257	0.00163
TAG 58:8/22:6	0.00091	0.00107	0.00035	0.00085	0.00028	0.00076	0.00081	0.00056	0.00048	0.00053	0.00048	0.00044	0.00111	0.00026	0.00090	0.00128	0.00182	0.00124
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
FC b
CE 16:0
CE 16:1
CE 18:0
CE 18:1
CE 18:2
CE 20:1
CE 20:2
CE 20:3
CE 20:4
CE 22:3
CE 22:4
CE 22:6
CE 24:6
AC C12:0
AC C14:0
AC C16:0
AC C18:0
AC C18:1
AC C2:0
AC C3:0
AC C6:0
AC C8:0
MG 16:0
MG 16:1
MG 18:0
MG 18:1
MG 18:2
MG 18:3
DAG 28:0/14:0
DAG 30:0/14:0
DAG 30:1/14:0
DAG 32:0/16:0
DAG 32:1/16:0
DAG 32:2/16:1
DAG 34:0/16:0
DAG 34:1/16:0
DAG 34:2/16:0
DAG 34:2/16:1
DAG 36:0/18:0
DAG 36:1/18:0
DAG 36:2/18:0
DAG 36:2/18:1
DAG 36:3/18:1
DAG 36:4/18:0
DAG 38:1/18:0
DAG 38:2/18:0
DAG 38:2/18:1
DAG 38:3/18:0
DAG 38:3/18:1
DAG 38:4/18:0
DAG 38:4/18:1
DAG 40:4/18:0
DAG 40:4/18:1
DAG 40:5/18:0
DAG 40:5/18:1
DAG 40:6/18:0
DAG 40:6/18:1
TAG 48:0/16:0
TAG 48:1/16:0
TAG 50:0/16:0
TAG 50:1/16:1
TAG 50:2/16:1
TAG 50:3/16:1
TAG 52:0/18:0
TAG 52:1/18:0
TAG 52:2/18:0
TAG 52:3/18:1
TAG 52:4/18:1
TAG 52:5/20:4
TAG 54:0/18:0
TAG 54:1/18:0
TAG 54:2/18:0
TAG 54:4/18:1
TAG 54:4/20:4
TAG 54:5/20:4
TAG 54:6/20:4
TAG 56:3/18:1
TAG 56:4/20:4
TAG 56:5/20:4
TAG 56:6/20:4
TAG 58:8/22:6
METABOLITES_END
#END