#METABOLOMICS WORKBENCH sczhang_20210701_132518_mwtab.txt DATATRACK_ID:2735 STUDY_ID:ST001867 ANALYSIS_ID:AN003029 PROJECT_ID:PR001179
VERSION             	1
CREATED_ON             	July 17, 2021, 6:49 pm
#PROJECT
PR:PROJECT_TITLE                 	Sodium dichloroacetate stimulates cardiac mitochondrial metabolism and improves
PR:PROJECT_TITLE                 	cardiac conduction in the ovine fetus during labor
PR:PROJECT_TYPE                  	untargeted NMR analysis
PR:PROJECT_SUMMARY               	Previous studies in our laboratory have suggested that the increase in
PR:PROJECT_SUMMARY               	stillbirth in pregnancies complicated by chronic maternal stress or
PR:PROJECT_SUMMARY               	hypercortisolemia is associated with cardiac dysfunction in late stages of labor
PR:PROJECT_SUMMARY               	and delivery. Transcriptomics analysis of the overly represented differentially
PR:PROJECT_SUMMARY               	expressed genes in the fetal heart of hypercortisolemic ewes indicated
PR:PROJECT_SUMMARY               	involvement of mitochondrial function. Sodium dichloroacetate (DCA) has been
PR:PROJECT_SUMMARY               	used to improve mitochondrial function in several disease states. We
PR:PROJECT_SUMMARY               	hypothesized that administration of DCA to laboring ewes would improve both
PR:PROJECT_SUMMARY               	cardiac mitochondrial activity and cardiac function in their fetuses. Four
PR:PROJECT_SUMMARY               	groups of ewes and their fetuses were studied: control, cortisol-infused (1
PR:PROJECT_SUMMARY               	g/kg/d from 115 to term; CORT), DCA-treated (over 24h) or DCA+CORT-treated;
PR:PROJECT_SUMMARY               	oxytocin was delivered starting 48h before the DCA treatment. DCA significantly
PR:PROJECT_SUMMARY               	decreased cardiac lactate, alanine and glucose/glucose-6-phosphate and increased
PR:PROJECT_SUMMARY               	acylcarnitine/isobutyryl-carnitine. DCA increased mitochondrial activity,
PR:PROJECT_SUMMARY               	increasing oxidative phosphorylation (PCI, PCI+II)) per tissue weight or per
PR:PROJECT_SUMMARY               	unit of citrate synthase. DCA also decreased the duration of the QRS,
PR:PROJECT_SUMMARY               	attenuating the prolongation of the QRS observed in CORT fetuses. The effect to
PR:PROJECT_SUMMARY               	reduce QRS duration with DCA treatment correlated with increased
PR:PROJECT_SUMMARY               	glycerophosphocholine and serine and decreased phophocholine after DCA
PR:PROJECT_SUMMARY               	treatment. There were negative correlations of
PR:PROJECT_SUMMARY               	acylcarnitine/isobutyryl-carnitine to both HR and MAP. These results suggest
PR:PROJECT_SUMMARY               	that improvements in mitochondrial respiration with DCA produced changes in the
PR:PROJECT_SUMMARY               	cardiac lipid metabolism that favor improved conduction in the heart. DCA may
PR:PROJECT_SUMMARY               	therefore be an effective treatment of fetal cardiac metabolic disturbances in
PR:PROJECT_SUMMARY               	labor that can contribute to impairments of fetal cardiac conduction.
PR:INSTITUTE                     	University of Georgia
PR:DEPARTMENT                    	Biochemistry and Molecular Biology and Complex Carbohydrate Research Center,
PR:DEPARTMENT                    	Department of Pharmacodynamics (University of Florida), Department of Physiology
PR:DEPARTMENT                    	and Functional Genomics (University of Florida)
PR:LABORATORY                    	Edison Lab, Keller-Wood Lab, and Wood Lab
PR:LAST_NAME                     	Edison
PR:FIRST_NAME                    	Arthur
PR:ADDRESS                       	315 Riverbend Road, Complex Carbohydrate Research Center, ATHENS, GA, 30605, USA
PR:EMAIL                         	aedison@uga.edu
PR:PHONE                         	NA
PR:CONTRIBUTORS                  	Serene Joseph, Mengchen Li, Sicong Zhang, Lloyd Horne, Peter. W. Stacpoole,
PR:CONTRIBUTORS                  	Stephanie E. Wohlgemuth, Arthur S. Edison, Charles Wood, Maureen Keller-Wood
#STUDY
ST:STUDY_TITLE                   	Sodium dichloroacetate stimulates cardiac mitochondrial metabolism and improves
ST:STUDY_TITLE                   	cardiac conduction in the ovine fetus during labor (part I)
ST:STUDY_SUMMARY                 	Previous studies in our laboratory have suggested that the increase in
ST:STUDY_SUMMARY                 	stillbirth in pregnancies complicated by chronic maternal stress or
ST:STUDY_SUMMARY                 	hypercortisolemia is associated with cardiac dysfunction in late stages of labor
ST:STUDY_SUMMARY                 	and delivery. Transcriptomics analysis of the overly represented differentially
ST:STUDY_SUMMARY                 	expressed genes in the fetal heart of hypercortisolemic ewes indicated
ST:STUDY_SUMMARY                 	involvement of mitochondrial function. Sodium dichloroacetate (DCA) has been
ST:STUDY_SUMMARY                 	used to improve mitochondrial function in several disease states. We
ST:STUDY_SUMMARY                 	hypothesized that administration of DCA to laboring ewes would improve both
ST:STUDY_SUMMARY                 	cardiac mitochondrial activity and cardiac function in their fetuses. Four
ST:STUDY_SUMMARY                 	groups of ewes and their fetuses were studied: control, cortisol-infused (1
ST:STUDY_SUMMARY                 	g/kg/d from 115 to term; CORT), DCA-treated (over 24h) or DCA+CORT-treated;
ST:STUDY_SUMMARY                 	oxytocin was delivered starting 48h before the DCA treatment. DCA significantly
ST:STUDY_SUMMARY                 	decreased cardiac lactate, alanine and glucose/glucose-6-phosphate and increased
ST:STUDY_SUMMARY                 	acylcarnitine/isobutyryl-carnitine. DCA increased mitochondrial activity,
ST:STUDY_SUMMARY                 	increasing oxidative phosphorylation (PCI, PCI+II)) per tissue weight or per
ST:STUDY_SUMMARY                 	unit of citrate synthase. DCA also decreased the duration of the QRS,
ST:STUDY_SUMMARY                 	attenuating the prolongation of the QRS observed in CORT fetuses. The effect to
ST:STUDY_SUMMARY                 	reduce QRS duration with DCA treatment correlated with increased
ST:STUDY_SUMMARY                 	glycerophosphocholine and serine and decreased phophocholine after DCA
ST:STUDY_SUMMARY                 	treatment. There were negative correlations of
ST:STUDY_SUMMARY                 	acylcarnitine/isobutyryl-carnitine to both HR and MAP. These results suggest
ST:STUDY_SUMMARY                 	that improvements in mitochondrial respiration with DCA produced changes in the
ST:STUDY_SUMMARY                 	cardiac lipid metabolism that favor improved conduction in the heart. DCA may
ST:STUDY_SUMMARY                 	therefore be an effective treatment of fetal cardiac metabolic disturbances in
ST:STUDY_SUMMARY                 	labor that can contribute to impairments of fetal cardiac conduction.
ST:INSTITUTE                     	University of Georgia
ST:DEPARTMENT                    	Biochemistry and Molecular Biology and Complex Carbohydrate Research Center,
ST:DEPARTMENT                    	Department of Pharmacodynamics (University of Florida), Department of Physiology
ST:DEPARTMENT                    	and Functional Genomics (University of Florida)
ST:LABORATORY                    	Edison Lab, Keller-Wood Lab, and Wood Lab
ST:LAST_NAME                     	Zhang
ST:FIRST_NAME                    	Sicong
ST:ADDRESS                       	315 Riverbend Road, Complex Carbohydrate Research Center
ST:EMAIL                         	sz91614@uga.edu
ST:NUM_GROUPS                    	4
ST:TOTAL_SUBJECTS                	29
ST:STUDY_TYPE                    	untargeted NMR analysis-cpmg
ST:PHONE                         	7067151662
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Ovis aries
SU:TAXONOMY_ID                   	9940
SU:ANIMAL_INCLUSION_CRITERIA     	Pregnant
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	1682	1682_L	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/39; Weight(mg)=30.01; MAPavglast3h=NaN; HRavglast3h=137; QRSAvg46=34; QRSavglast3=38; GA_surg=-43150; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=19-Feb-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=09-Jul-2018
SUBJECT_SAMPLE_FACTORS           	1428	1428_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/46; Weight(mg)=36.77; MAPavglast3h=49; HRavglast3h=150; QRSAvg46=41; QRSavglast3=48; GA_surg=128; GA_cort=116; GA_sac=143; cutofftime=26-Apr-2018 09:15:00; gender=m; fetus=s; breed=04-Dec-2017; cortdate=30-Mar-2018; surgery=11-Apr-2018; oxytocin=23-Apr-2018; oxytocintime=NaN; sac=26-Apr-2018
SUBJECT_SAMPLE_FACTORS           	1775	1775_L	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/40; Weight(mg)=34.43; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=19-Dec-2017 08:09:00; gender=f; fetus=triplet; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017
SUBJECT_SAMPLE_FACTORS           	1427	1427_L	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/41; Weight(mg)=34.52; MAPavglast3h=52; HRavglast3h=129; QRSAvg46=41; QRSavglast3=45; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=m; fetus=<undefined>; breed=03-Sep-2018; cortdate=no; surgery=04-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=23-Jan-2019
SUBJECT_SAMPLE_FACTORS           	1329	1329_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/5; Weight(mg)=29.91; MAPavglast3h=55; HRavglast3h=164; QRSAvg46=NaN; QRSavglast3=46; GA_surg=122; GA_cort=115; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Sep-2018; cortdate=17-Jan-2019; surgery=24-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=12-Feb-2019
SUBJECT_SAMPLE_FACTORS           	1261	1261_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/42; Weight(mg)=34.58; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=116; GA_sac=142; cutofftime=NaT; gender=m; fetus=s; breed=25-Sep-2017; cortdate=19-Jan-2018; surgery=30-Jan-2018; oxytocin=11-Feb-2018; oxytocintime=NaN; sac=14-Feb-2018
SUBJECT_SAMPLE_FACTORS           	1668	1668_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/7; Weight(mg)=22.7; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=36; QRSavglast3=38; GA_surg=120; GA_cort=115; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=25-Jun-2018; cortdate=18-Oct-2018; surgery=23-Oct-2018; oxytocin=NaT; oxytocintime=NaN; sac=12-Nov-2018
SUBJECT_SAMPLE_FACTORS           	1611	1611_L	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/44; Weight(mg)=38.19; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=130; GA_cort=-; GA_sac=144; cutofftime=NaT; gender=f; fetus=s; breed=15-Jan-2018; cortdate=no; surgery=25-May-2018; oxytocin=05-Jun-2018; oxytocintime=NaN; sac=08-Jun-2018
SUBJECT_SAMPLE_FACTORS           	1254	1254_L	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/10; Weight(mg)=47.54; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=37; QRSavglast3=37; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=01-Nov-2017 07:47:00; gender=m; fetus=s; breed=12-Jun-2017; cortdate=no; surgery=13-Oct-2017; oxytocin=29-Oct-2017; oxytocintime=NaN; sac=01-Nov-2017
SUBJECT_SAMPLE_FACTORS           	1565	1565_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/11; Weight(mg)=28.57; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43458; GA_cort=-43458; GA_sac=147; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Dec-2018; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=20-May-2019
SUBJECT_SAMPLE_FACTORS           	1719	1719_L	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/12; Weight(mg)=39.05; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=m; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.3125; sac=NaT
SUBJECT_SAMPLE_FACTORS           	1212	1212_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/13; Weight(mg)=29; MAPavglast3h=45; HRavglast3h=166; QRSAvg46=40; QRSavglast3=37; GA_surg=127; GA_cort=116; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=17-Dec-2018; cortdate=12-Apr-2019; surgery=23-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=07-May-2019
SUBJECT_SAMPLE_FACTORS           	1401	1401_L	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/14; Weight(mg)=31.93; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43472; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=07-Jan-2019; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.375; sac=27-May-2019
SUBJECT_SAMPLE_FACTORS           	1269	1269_L	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/15; Weight(mg)=27.66; MAPavglast3h=54; HRavglast3h=138; QRSAvg46=40; QRSavglast3=37; GA_surg=128; GA_cort=-; GA_sac=143; cutofftime=05-Apr-2018 09:09:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=21-Mar-2018; oxytocin=02-Apr-2018; oxytocintime=NaN; sac=05-Apr-2018
SUBJECT_SAMPLE_FACTORS           	3022	3022_L	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/38; Weight(mg)=18.67; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=13-Feb-2019
SUBJECT_SAMPLE_FACTORS           	1448	1448_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/17; Weight(mg)=38.18; MAPavglast3h=57; HRavglast3h=137; QRSAvg46=44; QRSavglast3=44; GA_surg=123; GA_cort=115; GA_sac=142; cutofftime=18-Apr-2018 16:12:00; gender=m; fetus=s; breed=27-Nov-2017; cortdate=22-Mar-2018; surgery=30-Mar-2018; oxytocin=16-Apr-2018; oxytocintime=NaN; sac=18-Apr-2018
SUBJECT_SAMPLE_FACTORS           	1727	1727_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/18; Weight(mg)=24; MAPavglast3h=48; HRavglast3h=163; QRSAvg46=52; QRSavglast3=45; GA_surg=127; GA_cort=115; GA_sac=142; cutofftime=28-Mar-2018 08:08:00; gender=f; fetus=s; breed=06-Nov-2017; cortdate=01-Mar-2018; surgery=13-Mar-2018; oxytocin=26-Mar-2018; oxytocintime=NaN; sac=28-Mar-2018
SUBJECT_SAMPLE_FACTORS           	1397	1397_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/21; Weight(mg)=24.18; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=NaT
SUBJECT_SAMPLE_FACTORS           	3019	3019_L	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/47; Weight(mg)=23.58; MAPavglast3h=48; HRavglast3h=143; QRSAvg46=41; QRSavglast3=43; GA_surg=NaN; GA_cort=-; GA_sac=141; cutofftime=NaT; gender=f; fetus=<undefined>; breed=10-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=29-Jan-2019
SUBJECT_SAMPLE_FACTORS           	1606	1606_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/37; Weight(mg)=26.15; MAPavglast3h=44; HRavglast3h=134; QRSAvg46=29; QRSavglast3=31; GA_surg=126; GA_cort=112; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=14-Jan-2019; cortdate=06-May-2019; surgery=20-May-2019; oxytocin=NaT; oxytocintime=0.375; sac=03-Jun-2019
SUBJECT_SAMPLE_FACTORS           	1775B	1775B_L	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/45; Weight(mg)=30.65; MAPavglast3h=66; HRavglast3h=172; QRSAvg46=NaN; QRSavglast3=36; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017
SUBJECT_SAMPLE_FACTORS           	1356	1356_L	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/26; Weight(mg)=26.53; MAPavglast3h=48; HRavglast3h=160; QRSAvg46=37; QRSavglast3=34; GA_surg=127; GA_cort=-; GA_sac=142; cutofftime=04-Apr-2018 08:11:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=20-Mar-2018; oxytocin=01-Apr-2018; oxytocintime=NaN; sac=04-Apr-2018
SUBJECT_SAMPLE_FACTORS           	1445	1445_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/27; Weight(mg)=32.16; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=40; QRSavglast3=NaN; GA_surg=127; GA_cort=NaN; GA_sac=141; cutofftime=NaT; gender=f; fetus=s; breed=10-Dec-2018; cortdate=<undefined>; surgery=16-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=30-Apr-2019
SUBJECT_SAMPLE_FACTORS           	1324	1324_L	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/28; Weight(mg)=40.51; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=143; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Dec-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=16-May-2019
SUBJECT_SAMPLE_FACTORS           	1642	1642_L	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/29; Weight(mg)=41.71; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=NaN; GA_sac=143; cutofftime=NaT; gender=m; fetus=<undefined>; breed=05-Feb-2018; cortdate=<undefined>; surgery=14-Jun-2018; oxytocin=NaT; oxytocintime=NaN; sac=28-Jun-2018
SUBJECT_SAMPLE_FACTORS           	1603	1603_L	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/30; Weight(mg)=46.19; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=01-Feb-2018 08:08:00; gender=<undefined>; fetus=s; breed=11-Sep-2017; cortdate=no; surgery=16-Jan-2018; oxytocin=29-Jan-2018; oxytocintime=NaN; sac=01-Feb-2018
SUBJECT_SAMPLE_FACTORS           	1277	1277_L	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/31; Weight(mg)=36.29; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=18-Jan-2018 08:10:00; gender=m; fetus=s; breed=28-Aug-2017; cortdate=no; surgery=02-Jan-2018; oxytocin=15-Jan-2018; oxytocintime=NaN; sac=18-Jan-2018
SUBJECT_SAMPLE_FACTORS           	1532	1532_L	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/32; Weight(mg)=32.83; MAPavglast3h=57; HRavglast3h=149; QRSAvg46=41; QRSavglast3=41; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=15-Mar-2018 08:13:00; gender=f; fetus=s; breed=23-Oct-2017; cortdate=no; surgery=27-Feb-2018; oxytocin=12-Mar-2018; oxytocintime=NaN; sac=15-Mar-2018
SUBJECT_SAMPLE_FACTORS           	1656	1656_L	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_L_03172020/33; Weight(mg)=35.07; MAPavglast3h=63; HRavglast3h=159; QRSAvg46=NaN; QRSavglast3=37; GA_surg=133; GA_cort=-; GA_sac=141; cutofftime=07-Nov-2017 13:44:00; gender=f; fetus=t; breed=19-Jun-2017; cortdate=no; surgery=30-Oct-2017; oxytocin=05-Nov-2017; oxytocintime=NaN; sac=07-Nov-2017
SUBJECT_SAMPLE_FACTORS           	1682	1682_R	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/41; Weight(mg)=25.45; MAPavglast3h=NaN; HRavglast3h=137; QRSAvg46=34; QRSavglast3=38; GA_surg=-43150; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=19-Feb-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=09-Jul-2018
SUBJECT_SAMPLE_FACTORS           	1428	1428_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/42; Weight(mg)=28.41; MAPavglast3h=49; HRavglast3h=150; QRSAvg46=41; QRSavglast3=48; GA_surg=128; GA_cort=116; GA_sac=143; cutofftime=26-Apr-2018 09:15:00; gender=m; fetus=s; breed=04-Dec-2017; cortdate=30-Mar-2018; surgery=11-Apr-2018; oxytocin=23-Apr-2018; oxytocintime=NaN; sac=26-Apr-2018
SUBJECT_SAMPLE_FACTORS           	1775	1775_R	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/47; Weight(mg)=36.61; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=19-Dec-2017 08:09:00; gender=f; fetus=triplet; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017
SUBJECT_SAMPLE_FACTORS           	1427	1427_R	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/43; Weight(mg)=28.67; MAPavglast3h=52; HRavglast3h=129; QRSAvg46=41; QRSavglast3=45; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=m; fetus=<undefined>; breed=03-Sep-2018; cortdate=no; surgery=04-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=23-Jan-2019
SUBJECT_SAMPLE_FACTORS           	1329	1329_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/49; Weight(mg)=32.57; MAPavglast3h=55; HRavglast3h=164; QRSAvg46=NaN; QRSavglast3=46; GA_surg=122; GA_cort=115; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Sep-2018; cortdate=17-Jan-2019; surgery=24-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=12-Feb-2019
SUBJECT_SAMPLE_FACTORS           	1261	1261_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/50; Weight(mg)=29.35; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=116; GA_sac=142; cutofftime=NaT; gender=m; fetus=s; breed=25-Sep-2017; cortdate=19-Jan-2018; surgery=30-Jan-2018; oxytocin=11-Feb-2018; oxytocintime=NaN; sac=14-Feb-2018
SUBJECT_SAMPLE_FACTORS           	1668	1668_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/52; Weight(mg)=33.77; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=36; QRSavglast3=38; GA_surg=120; GA_cort=115; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=25-Jun-2018; cortdate=18-Oct-2018; surgery=23-Oct-2018; oxytocin=NaT; oxytocintime=NaN; sac=12-Nov-2018
SUBJECT_SAMPLE_FACTORS           	1611	1611_R	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/14; Weight(mg)=27.94; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=130; GA_cort=-; GA_sac=144; cutofftime=NaT; gender=f; fetus=s; breed=15-Jan-2018; cortdate=no; surgery=25-May-2018; oxytocin=05-Jun-2018; oxytocintime=NaN; sac=08-Jun-2018
SUBJECT_SAMPLE_FACTORS           	1254	1254_R	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/16; Weight(mg)=37.24; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=37; QRSavglast3=37; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=01-Nov-2017 07:47:00; gender=m; fetus=s; breed=12-Jun-2017; cortdate=no; surgery=13-Oct-2017; oxytocin=29-Oct-2017; oxytocintime=NaN; sac=01-Nov-2017
SUBJECT_SAMPLE_FACTORS           	1565	1565_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/18; Weight(mg)=36.98; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43458; GA_cort=-43458; GA_sac=147; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Dec-2018; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=20-May-2019
SUBJECT_SAMPLE_FACTORS           	1719	1719_R	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/19; Weight(mg)=25.24; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=m; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.3125; sac=NaT
SUBJECT_SAMPLE_FACTORS           	1212	1212_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/20; Weight(mg)=28.3; MAPavglast3h=45; HRavglast3h=166; QRSAvg46=40; QRSavglast3=37; GA_surg=127; GA_cort=116; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=17-Dec-2018; cortdate=12-Apr-2019; surgery=23-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=07-May-2019
SUBJECT_SAMPLE_FACTORS           	1401	1401_R	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/21; Weight(mg)=37.14; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43472; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=07-Jan-2019; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.375; sac=27-May-2019
SUBJECT_SAMPLE_FACTORS           	1269	1269_R	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/22; Weight(mg)=32.17; MAPavglast3h=54; HRavglast3h=138; QRSAvg46=40; QRSavglast3=37; GA_surg=128; GA_cort=-; GA_sac=143; cutofftime=05-Apr-2018 09:09:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=21-Mar-2018; oxytocin=02-Apr-2018; oxytocintime=NaN; sac=05-Apr-2018
SUBJECT_SAMPLE_FACTORS           	3022	3022_R	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/23; Weight(mg)=27.71; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=13-Feb-2019
SUBJECT_SAMPLE_FACTORS           	1448	1448_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/24; Weight(mg)=26.72; MAPavglast3h=57; HRavglast3h=137; QRSAvg46=44; QRSavglast3=44; GA_surg=123; GA_cort=115; GA_sac=142; cutofftime=18-Apr-2018 16:12:00; gender=m; fetus=s; breed=27-Nov-2017; cortdate=22-Mar-2018; surgery=30-Mar-2018; oxytocin=16-Apr-2018; oxytocintime=NaN; sac=18-Apr-2018
SUBJECT_SAMPLE_FACTORS           	1727	1727_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/25; Weight(mg)=32.58; MAPavglast3h=48; HRavglast3h=163; QRSAvg46=52; QRSavglast3=45; GA_surg=127; GA_cort=115; GA_sac=142; cutofftime=28-Mar-2018 08:08:00; gender=f; fetus=s; breed=06-Nov-2017; cortdate=01-Mar-2018; surgery=13-Mar-2018; oxytocin=26-Mar-2018; oxytocintime=NaN; sac=28-Mar-2018
SUBJECT_SAMPLE_FACTORS           	1397	1397_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/26; Weight(mg)=35.43; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=NaT
SUBJECT_SAMPLE_FACTORS           	3019	3019_R	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/28; Weight(mg)=40.04; MAPavglast3h=48; HRavglast3h=143; QRSAvg46=41; QRSavglast3=43; GA_surg=NaN; GA_cort=-; GA_sac=141; cutofftime=NaT; gender=f; fetus=<undefined>; breed=10-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=29-Jan-2019
SUBJECT_SAMPLE_FACTORS           	1606	1606_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/29; Weight(mg)=36.34; MAPavglast3h=44; HRavglast3h=134; QRSAvg46=29; QRSavglast3=31; GA_surg=126; GA_cort=112; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=14-Jan-2019; cortdate=06-May-2019; surgery=20-May-2019; oxytocin=NaT; oxytocintime=0.375; sac=03-Jun-2019
SUBJECT_SAMPLE_FACTORS           	1775B	1775B_R	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/30; Weight(mg)=33.61; MAPavglast3h=66; HRavglast3h=172; QRSAvg46=NaN; QRSavglast3=36; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017
SUBJECT_SAMPLE_FACTORS           	1356	1356_R	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/31; Weight(mg)=25.96; MAPavglast3h=48; HRavglast3h=160; QRSAvg46=37; QRSavglast3=34; GA_surg=127; GA_cort=-; GA_sac=142; cutofftime=04-Apr-2018 08:11:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=20-Mar-2018; oxytocin=01-Apr-2018; oxytocintime=NaN; sac=04-Apr-2018
SUBJECT_SAMPLE_FACTORS           	1445	1445_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/32; Weight(mg)=35.29; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=40; QRSavglast3=NaN; GA_surg=127; GA_cort=NaN; GA_sac=141; cutofftime=NaT; gender=f; fetus=s; breed=10-Dec-2018; cortdate=<undefined>; surgery=16-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=30-Apr-2019
SUBJECT_SAMPLE_FACTORS           	1324	1324_R	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/33; Weight(mg)=34.31; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=143; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Dec-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=16-May-2019
SUBJECT_SAMPLE_FACTORS           	1642	1642_R	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/34; Weight(mg)=36.25; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=NaN; GA_sac=143; cutofftime=NaT; gender=m; fetus=<undefined>; breed=05-Feb-2018; cortdate=<undefined>; surgery=14-Jun-2018; oxytocin=NaT; oxytocintime=NaN; sac=28-Jun-2018
SUBJECT_SAMPLE_FACTORS           	1603	1603_R	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/35; Weight(mg)=30.37; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=01-Feb-2018 08:08:00; gender=<undefined>; fetus=s; breed=11-Sep-2017; cortdate=no; surgery=16-Jan-2018; oxytocin=29-Jan-2018; oxytocintime=NaN; sac=01-Feb-2018
SUBJECT_SAMPLE_FACTORS           	1277	1277_R	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/38; Weight(mg)=38.22; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=18-Jan-2018 08:10:00; gender=m; fetus=s; breed=28-Aug-2017; cortdate=no; surgery=02-Jan-2018; oxytocin=15-Jan-2018; oxytocintime=NaN; sac=18-Jan-2018
SUBJECT_SAMPLE_FACTORS           	1532	1532_R	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/39; Weight(mg)=37.77; MAPavglast3h=57; HRavglast3h=149; QRSAvg46=41; QRSavglast3=41; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=15-Mar-2018 08:13:00; gender=f; fetus=s; breed=23-Oct-2017; cortdate=no; surgery=27-Feb-2018; oxytocin=12-Mar-2018; oxytocintime=NaN; sac=15-Mar-2018
SUBJECT_SAMPLE_FACTORS           	1656	1656_R	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_R_06222020/40; Weight(mg)=36.65; MAPavglast3h=63; HRavglast3h=159; QRSAvg46=NaN; QRSavglast3=37; GA_surg=133; GA_cort=-; GA_sac=141; cutofftime=07-Nov-2017 13:44:00; gender=f; fetus=t; breed=19-Jun-2017; cortdate=no; surgery=30-Oct-2017; oxytocin=05-Nov-2017; oxytocintime=NaN; sac=07-Nov-2017
SUBJECT_SAMPLE_FACTORS           	1682	1682_S	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/10; Weight(mg)=34.02; MAPavglast3h=NaN; HRavglast3h=137; QRSAvg46=34; QRSavglast3=38; GA_surg=-43150; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=19-Feb-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=09-Jul-2018
SUBJECT_SAMPLE_FACTORS           	1428	1428_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/4; Weight(mg)=26.89; MAPavglast3h=49; HRavglast3h=150; QRSAvg46=41; QRSavglast3=48; GA_surg=128; GA_cort=116; GA_sac=143; cutofftime=26-Apr-2018 09:15:00; gender=m; fetus=s; breed=04-Dec-2017; cortdate=30-Mar-2018; surgery=11-Apr-2018; oxytocin=23-Apr-2018; oxytocintime=NaN; sac=26-Apr-2018
SUBJECT_SAMPLE_FACTORS           	1775	1775_S	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/9; Weight(mg)=29.54; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=19-Dec-2017 08:09:00; gender=f; fetus=triplet; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017
SUBJECT_SAMPLE_FACTORS           	1427	1427_S	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/7; Weight(mg)=38.24; MAPavglast3h=52; HRavglast3h=129; QRSAvg46=41; QRSavglast3=45; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=m; fetus=<undefined>; breed=03-Sep-2018; cortdate=no; surgery=04-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=23-Jan-2019
SUBJECT_SAMPLE_FACTORS           	1329	1329_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/8; Weight(mg)=31.7; MAPavglast3h=55; HRavglast3h=164; QRSAvg46=NaN; QRSavglast3=46; GA_surg=122; GA_cort=115; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Sep-2018; cortdate=17-Jan-2019; surgery=24-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=12-Feb-2019
SUBJECT_SAMPLE_FACTORS           	1261	1261_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/12; Weight(mg)=43.65; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=116; GA_sac=142; cutofftime=NaT; gender=m; fetus=s; breed=25-Sep-2017; cortdate=19-Jan-2018; surgery=30-Jan-2018; oxytocin=11-Feb-2018; oxytocintime=NaN; sac=14-Feb-2018
SUBJECT_SAMPLE_FACTORS           	1668	1668_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/13; Weight(mg)=40.14; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=36; QRSavglast3=38; GA_surg=120; GA_cort=115; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=25-Jun-2018; cortdate=18-Oct-2018; surgery=23-Oct-2018; oxytocin=NaT; oxytocintime=NaN; sac=12-Nov-2018
SUBJECT_SAMPLE_FACTORS           	1611	1611_S	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/14; Weight(mg)=44.28; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=130; GA_cort=-; GA_sac=144; cutofftime=NaT; gender=f; fetus=s; breed=15-Jan-2018; cortdate=no; surgery=25-May-2018; oxytocin=05-Jun-2018; oxytocintime=NaN; sac=08-Jun-2018
SUBJECT_SAMPLE_FACTORS           	1254	1254_S	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/15; Weight(mg)=39.48; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=37; QRSavglast3=37; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=01-Nov-2017 07:47:00; gender=m; fetus=s; breed=12-Jun-2017; cortdate=no; surgery=13-Oct-2017; oxytocin=29-Oct-2017; oxytocintime=NaN; sac=01-Nov-2017
SUBJECT_SAMPLE_FACTORS           	1565	1565_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/16; Weight(mg)=34.15; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43458; GA_cort=-43458; GA_sac=147; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Dec-2018; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=20-May-2019
SUBJECT_SAMPLE_FACTORS           	1719	1719_S	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/17; Weight(mg)=31.81; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=m; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.3125; sac=NaT
SUBJECT_SAMPLE_FACTORS           	1212	1212_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/18; Weight(mg)=36.17; MAPavglast3h=45; HRavglast3h=166; QRSAvg46=40; QRSavglast3=37; GA_surg=127; GA_cort=116; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=17-Dec-2018; cortdate=12-Apr-2019; surgery=23-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=07-May-2019
SUBJECT_SAMPLE_FACTORS           	1401	1401_S	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/19; Weight(mg)=30.83; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43472; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=07-Jan-2019; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.375; sac=27-May-2019
SUBJECT_SAMPLE_FACTORS           	1269	1269_S	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/21; Weight(mg)=35.45; MAPavglast3h=54; HRavglast3h=138; QRSAvg46=40; QRSavglast3=37; GA_surg=128; GA_cort=-; GA_sac=143; cutofftime=05-Apr-2018 09:09:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=21-Mar-2018; oxytocin=02-Apr-2018; oxytocintime=NaN; sac=05-Apr-2018
SUBJECT_SAMPLE_FACTORS           	3022	3022_S	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/22; Weight(mg)=26.2; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=13-Feb-2019
SUBJECT_SAMPLE_FACTORS           	1448	1448_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/23; Weight(mg)=28.16; MAPavglast3h=57; HRavglast3h=137; QRSAvg46=44; QRSavglast3=44; GA_surg=123; GA_cort=115; GA_sac=142; cutofftime=18-Apr-2018 16:12:00; gender=m; fetus=s; breed=27-Nov-2017; cortdate=22-Mar-2018; surgery=30-Mar-2018; oxytocin=16-Apr-2018; oxytocintime=NaN; sac=18-Apr-2018
SUBJECT_SAMPLE_FACTORS           	1727	1727_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/24; Weight(mg)=28.43; MAPavglast3h=48; HRavglast3h=163; QRSAvg46=52; QRSavglast3=45; GA_surg=127; GA_cort=115; GA_sac=142; cutofftime=28-Mar-2018 08:08:00; gender=f; fetus=s; breed=06-Nov-2017; cortdate=01-Mar-2018; surgery=13-Mar-2018; oxytocin=26-Mar-2018; oxytocintime=NaN; sac=28-Mar-2018
SUBJECT_SAMPLE_FACTORS           	1397	1397_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/25; Weight(mg)=31.9; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=NaT
SUBJECT_SAMPLE_FACTORS           	3019	3019_S	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/26; Weight(mg)=31.46; MAPavglast3h=48; HRavglast3h=143; QRSAvg46=41; QRSavglast3=43; GA_surg=NaN; GA_cort=-; GA_sac=141; cutofftime=NaT; gender=f; fetus=<undefined>; breed=10-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=29-Jan-2019
SUBJECT_SAMPLE_FACTORS           	1606	1606_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/27; Weight(mg)=28.72; MAPavglast3h=44; HRavglast3h=134; QRSAvg46=29; QRSavglast3=31; GA_surg=126; GA_cort=112; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=14-Jan-2019; cortdate=06-May-2019; surgery=20-May-2019; oxytocin=NaT; oxytocintime=0.375; sac=03-Jun-2019
SUBJECT_SAMPLE_FACTORS           	1775B	1775B_S	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/28; Weight(mg)=45.09; MAPavglast3h=66; HRavglast3h=172; QRSAvg46=NaN; QRSavglast3=36; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017
SUBJECT_SAMPLE_FACTORS           	1356	1356_S	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/29; Weight(mg)=34.64; MAPavglast3h=48; HRavglast3h=160; QRSAvg46=37; QRSavglast3=34; GA_surg=127; GA_cort=-; GA_sac=142; cutofftime=04-Apr-2018 08:11:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=20-Mar-2018; oxytocin=01-Apr-2018; oxytocintime=NaN; sac=04-Apr-2018
SUBJECT_SAMPLE_FACTORS           	1445	1445_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/31; Weight(mg)=36.72; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=40; QRSavglast3=NaN; GA_surg=127; GA_cort=NaN; GA_sac=141; cutofftime=NaT; gender=f; fetus=s; breed=10-Dec-2018; cortdate=<undefined>; surgery=16-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=30-Apr-2019
SUBJECT_SAMPLE_FACTORS           	1324	1324_S	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/32; Weight(mg)=42.25; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=143; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Dec-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=16-May-2019
SUBJECT_SAMPLE_FACTORS           	1642	1642_S	Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/33; Weight(mg)=36.16; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=NaN; GA_sac=143; cutofftime=NaT; gender=m; fetus=<undefined>; breed=05-Feb-2018; cortdate=<undefined>; surgery=14-Jun-2018; oxytocin=NaT; oxytocintime=NaN; sac=28-Jun-2018
SUBJECT_SAMPLE_FACTORS           	1603	1603_S	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/34; Weight(mg)=45.64; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=01-Feb-2018 08:08:00; gender=<undefined>; fetus=s; breed=11-Sep-2017; cortdate=no; surgery=16-Jan-2018; oxytocin=29-Jan-2018; oxytocintime=NaN; sac=01-Feb-2018
SUBJECT_SAMPLE_FACTORS           	1277	1277_S	Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h)	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/35; Weight(mg)=28.17; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=18-Jan-2018 08:10:00; gender=m; fetus=s; breed=28-Aug-2017; cortdate=no; surgery=02-Jan-2018; oxytocin=15-Jan-2018; oxytocintime=NaN; sac=18-Jan-2018
SUBJECT_SAMPLE_FACTORS           	1532	1532_S	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/36; Weight(mg)=28.29; MAPavglast3h=57; HRavglast3h=149; QRSAvg46=41; QRSavglast3=41; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=15-Mar-2018 08:13:00; gender=f; fetus=s; breed=23-Oct-2017; cortdate=no; surgery=27-Feb-2018; oxytocin=12-Mar-2018; oxytocintime=NaN; sac=15-Mar-2018
SUBJECT_SAMPLE_FACTORS           	1656	1656_S	Cortisol treatment:Control | DCA treatment:Control	RAW_FILE_NAME=Sheep_heart_cpmg_SZ_S_06302020/37; Weight(mg)=29.92; MAPavglast3h=63; HRavglast3h=159; QRSAvg46=NaN; QRSavglast3=37; GA_surg=133; GA_cort=-; GA_sac=141; cutofftime=07-Nov-2017 13:44:00; gender=f; fetus=t; breed=19-Jun-2017; cortdate=no; surgery=30-Oct-2017; oxytocin=05-Nov-2017; oxytocintime=NaN; sac=07-Nov-2017
#COLLECTION
CO:COLLECTION_SUMMARY            	Heart tissue was collected from the right ventricle, left ventricle, and
CO:COLLECTION_SUMMARY            	intraventricular septum and immediately frozen in liquid nitrogen.
CO:SAMPLE_TYPE                   	Heart
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	Animals in the CORT and CORT+DCA groups were implanted with pellets of cortisol
TR:TREATMENT_SUMMARY             	hemisuccinate (Innovative Research, Sarasota, FL) or infused with cortisol
TR:TREATMENT_SUMMARY             	hemisuccinate (Solu-Cortef, Pfizer, Inc) using an ambulatory pump (3D Micro
TR:TREATMENT_SUMMARY             	Infusion Pump; Strategic Applications Inc.; Lake Villa, IL) to provide dosing of
TR:TREATMENT_SUMMARY             	cortisol at 1mg/kg/day beginning on day 115-116 of gestation until the end of
TR:TREATMENT_SUMMARY             	the experiment when the animals were sacrificed. This dose of cortisol increased
TR:TREATMENT_SUMMARY             	maternal cortisol concentrations to approximately 1.5-fold, mimicking the rise
TR:TREATMENT_SUMMARY             	in cortisol in moderate stress. To produce a regular pattern of labor, ewes were
TR:TREATMENT_SUMMARY             	infused with oxytocin (Aspen Veterinary Resources) beginning at day 138-140 of
TR:TREATMENT_SUMMARY             	gestation; oxytocin was infused over 5 minutes every 30 minutes (820 µU/kg/min
TR:TREATMENT_SUMMARY             	iv;) using an infusion pump controlled by a timed controller ChronTrol Corp,
TR:TREATMENT_SUMMARY             	Inc). DCA infusion was started 48 hours after the start of oxytocin. DCA was
TR:TREATMENT_SUMMARY             	administered as an intravenous bolus of 25 mg/kg over 3 minutes, followed by an
TR:TREATMENT_SUMMARY             	infusion of 12.5 mg DCA/kg/hour for 8 hours, and an infusion of 6.25 mg DCA/kg/h
TR:TREATMENT_SUMMARY             	for the next 8 hours. These doses are similar to those that significantly
TR:TREATMENT_SUMMARY             	decrease circulating lactate concentrations in humans. The timed infusions of
TR:TREATMENT_SUMMARY             	oxytocin continued for 72 hours, and the study was terminated after 24 hours of
TR:TREATMENT_SUMMARY             	DCA and/or 72 hours of oxytocin infusion, unless ewes were in the process of
TR:TREATMENT_SUMMARY             	delivery or birth occurred.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Heart tissues were cut to ~ 30 mg and weighted. Each tissue was added to 30 µl
SP:SAMPLEPREP_SUMMARY            	of D2O with 10/3 mM sodium trimethylsilylpropane-sulfonate (DSS) and transferred
SP:SAMPLEPREP_SUMMARY            	to a 4-mm zirconium dioxide rotor with Kel-F cap and PTFE spacer provided by
SP:SAMPLEPREP_SUMMARY            	Bruker Biospin. Samples were kept on dry ice during sample preparation.
#ANALYSIS
AN:ANALYSIS_TYPE                 	NMR
AN:LABORATORY_NAME               	Edison Lab
AN:SOFTWARE_VERSION              	Topspin 4.0.6
#NMR
NM:INSTRUMENT_NAME               	Bruker NEO 600MHz
NM:INSTRUMENT_TYPE               	FT-NMR
NM:NMR_EXPERIMENT_TYPE           	1D-1H
NM:FIELD_FREQUENCY_LOCK          	D2O
NM:SPECTROMETER_FREQUENCY        	600 MHz
NM:NMR_PROBE                     	4mm CMP_HRMAS
NM:NMR_SOLVENT                   	D2O
NM:NMR_TUBE_SIZE                 	50 ul
NM:SHIMMING_METHOD               	manual
NM:PULSE_SEQUENCE                	cpmgpr1d
NM:WATER_SUPPRESSION             	presat
NM:RECEIVER_GAIN                 	101
NM:CHEMICAL_SHIFT_REF_CPD        	DSS
NM:TEMPERATURE                   	10
NM:NUMBER_OF_SCANS               	128
NM:DUMMY_SCANS                   	8
NM:ACQUISITION_TIME              	0.69 s
NM:SPECTRAL_WIDTH                	19.8393
NM:NUM_DATA_POINTS_ACQUIRED      	16384
NM:LINE_BROADENING               	1.8
NM:BASELINE_CORRECTION_METHOD    	ABSG
NM:NMR_RESULTS_FILE              	CPMG_binned_data.txt	UNITS:(relative)
#END