#METABOLOMICS WORKBENCH severinezirah_20211113_140942_mwtab.txt DATATRACK_ID:2928 STUDY_ID:ST002014 ANALYSIS_ID:AN003282 PROJECT_ID:PR001278 VERSION 1 CREATED_ON November 18, 2021, 8:53 am #PROJECT PR:PROJECT_TITLE PolyFerm PR:PROJECT_SUMMARY The increased prevalence of Salmonella spp. resistance in swine spurs the search PR:PROJECT_SUMMARY for alternatives to antibiotics. Microcin J25 (MccJ25), a bacteriocin produced PR:PROJECT_SUMMARY by Escherichia coli, is a potent inhibitor of several pathogenic bacteria PR:PROJECT_SUMMARY including Salmonella enterica. In this study, we aimed to evaluate in vitro the PR:PROJECT_SUMMARY impact of MccJ25 on the metabolic activity of the swine colonic microbiota. The PR:PROJECT_SUMMARY PolyFermS in vitro continuous fermentation model was used with modified PR:PROJECT_SUMMARY Macfarlane medium to simulate the porcine proximal colon. During 35 days of PR:PROJECT_SUMMARY fermentation, a first-stage reactor containing immobilized swine fecal PR:PROJECT_SUMMARY microbiota fed two second-stage control and test reactors operated in parallel PR:PROJECT_SUMMARY and used to test the effectsof MccJ25 on the composition and the metabolic PR:PROJECT_SUMMARY activity of the microbiota. Reuterin, a broad spectrum antimicrobial produced by PR:PROJECT_SUMMARY Limosilactobacillus reuteri and the antibiotic rifampicin were tested for PR:PROJECT_SUMMARY comparison. LC-MS analysis of the cell extracts was used to assess the PR:PROJECT_SUMMARY bacteriocin/antibiotic degradation products and monitor changes in the swine PR:PROJECT_SUMMARY colonic microbiota metabolome. PR:INSTITUTE National Museum of Natural History PR:LABORATORY Communication Molecules and Adaptation of Microorganisms PR:LAST_NAME Zirah PR:FIRST_NAME Severine PR:ADDRESS Museum national d'Histoire naturelle, Unité MCAM UMR 7245 CNRS-MNHN, CP 54, 57 PR:ADDRESS rue Cuvier, 75005 Paris, FRANCE PR:EMAIL severine.zirah@mnhn.fr PR:PHONE +33(0)1 40 79 31 40 #STUDY ST:STUDY_TITLE Impact of microcin J25 on the porcine microbiome in a continuous culture model ST:STUDY_SUMMARY The increased prevalence of Salmonella spp. resistance in swine spurs the search ST:STUDY_SUMMARY for alternatives to antibiotics. Microcin J25 (MccJ25), a bacteriocin produced ST:STUDY_SUMMARY by Escherichia coli, is a potent inhibitor of several pathogenic bacteria ST:STUDY_SUMMARY including Salmonella enterica. In this study, we aimed to evaluate in vitro the ST:STUDY_SUMMARY impact of MccJ25 on the metabolic activity of the swine colonic microbiota. The ST:STUDY_SUMMARY PolyFermS in vitro continuous fermentation model was used with modified ST:STUDY_SUMMARY Macfarlane medium to simulate the porcine proximal colon. During 35 days of ST:STUDY_SUMMARY fermentation, a first-stage reactor containing immobilized swine fecal ST:STUDY_SUMMARY microbiota fed two second-stage control and test reactors operated in parallel ST:STUDY_SUMMARY and used to test the effectsof MccJ25 on the composition and the metabolic ST:STUDY_SUMMARY activity of the microbiota. Reuterin, a broad spectrum antimicrobial produced by ST:STUDY_SUMMARY Limosilactobacillus reuteri and the antibiotic rifampicin were tested for ST:STUDY_SUMMARY comparison. LC-MS analysis of the cell extracts was used to assess the ST:STUDY_SUMMARY bacteriocin/antibiotic degradation products and monitor changes in the swine ST:STUDY_SUMMARY colonic microbiota metabolome. ST:INSTITUTE National Museum of Natural History ST:LAST_NAME Zirah ST:FIRST_NAME Severine ST:ADDRESS Museum national d'Histoire naturelle, Unité MCAM UMR 7245 CNRS-MNHN, CP 54, 57 ST:ADDRESS rue Cuvier, 75005 Paris, FRANCE ST:EMAIL severine.zirah@mnhn.fr ST:PHONE +33(0)1 40 79 31 40 ST:NUM_GROUPS 6 #SUBJECT SU:SUBJECT_TYPE Bacteria SU:SUBJECT_SPECIES swine colonic microbiota SU:GENDER Not applicable #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - SalmoF2_t2_RA2_01_3057 group:Salmo | ferm:F2 RAW_FILE_NAME=100_Intra_SalmoF2_t2_RA2_01_3057.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t6_RA3_01_3060 group:Salmo | ferm:F1 RAW_FILE_NAME=101_Intra_SalmoF1_t6_RA3_01_3060.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t-1_RA4_01_3061 group:Stab | ferm:F1 RAW_FILE_NAME=102_Intra_RifaF1_t-1_RA4_01_3061.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t6_RA5_01_3062 group:Reut | ferm:F2 RAW_FILE_NAME=103_Intra_ReutF2_t6_RA5_01_3062.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J16_RA6_01_3063 group:Stab | ferm:F1 RAW_FILE_NAME=104_Intra_StabF1_J16_RA6_01_3063.cdf; time=NA; time_stab=16 SUBJECT_SAMPLE_FACTORS - RifaF1_t0_RA7_01_3064 group:Rifa | ferm:F1 RAW_FILE_NAME=105_Intra_RifaF1_t0_RA7_01_3064.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t0_RA8_01_3065 group:J25 | ferm:F2 RAW_FILE_NAME=106_Intra_J25F2_t0_RA8_01_3065.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t24_RB1_01_3066 group:J25 | ferm:F1 RAW_FILE_NAME=107_Intra_J25F1_t24_RB1_01_3066.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t8_RB2_01_3067 group:Reut | ferm:F1 RAW_FILE_NAME=108_Intra_ReutF1_t8_RB2_01_3067.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t0_RB3_01_3068 group:Reut | ferm:F1 RAW_FILE_NAME=109_Intra_ReutF1_t0_RB3_01_3068.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t10_RB4_01_3069 group:Reut | ferm:F2 RAW_FILE_NAME=110_Intra_ReutF2_t10_RB4_01_3069.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t10_RB5_01_3070 group:Reut | ferm:F1 RAW_FILE_NAME=111_Intra_ReutF1_t10_RB5_01_3070.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t6_RB6_01_3071 group:Reut | ferm:F2 RAW_FILE_NAME=112_Intra_ReutF2_t6_RB6_01_3071.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t12_RB7_01_3072 group:J25 | ferm:F2 RAW_FILE_NAME=113_Intra_J25F2_t12_RB7_01_3072.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t4_RB8_01_3073 group:J25 | ferm:F1 RAW_FILE_NAME=114_Intra_J25F1_t4_RB8_01_3073.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t10_RC1_01_3074 group:Rifa | ferm:F1 RAW_FILE_NAME=115_Intra_RifaF1_t10_RC1_01_3074.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF2_t2_RC2_01_3075 group:Rifa | ferm:F2 RAW_FILE_NAME=116_Intra_RifaF2_t2_RC2_01_3075.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J27_RC3_01_3078 group:Stab | ferm:F2 RAW_FILE_NAME=117_Intra_StabF2_J27_RC3_01_3078.cdf; time=NA; time_stab=27 SUBJECT_SAMPLE_FACTORS - ReutF1_t0_RC4_01_3079 group:Reut | ferm:F1 RAW_FILE_NAME=118_Intra_ReutF1_t0_RC4_01_3079.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t2_RC5_01_3080 group:Reut | ferm:F2 RAW_FILE_NAME=119_Intra_ReutF2_t2_RC5_01_3080.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t2_RC6_01_3081 group:Rifa | ferm:F1 RAW_FILE_NAME=120_Intra_RifaF1_t2_RC6_01_3081.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t8_RC7_01_3082 group:Salmo | ferm:F2 RAW_FILE_NAME=121_Intra_SalmoF2_t8_RC7_01_3082.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t6_RC8_01_3083 group:Salmo | ferm:F2 RAW_FILE_NAME=122_Intra_SalmoF2_t6_RC8_01_3083.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J23_RD1_01_3084 group:Stab | ferm:F2 RAW_FILE_NAME=122_Intra_StabF2_J23_RD1_01_3084.cdf; time=NA; time_stab=23 SUBJECT_SAMPLE_FACTORS - ReutF2_t10_RD2_01_3085 group:Reut | ferm:F2 RAW_FILE_NAME=123_Intra_ReutF2_t10_RD2_01_3085.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t12_RD3_01_3086 group:J25 | ferm:F2 RAW_FILE_NAME=124_Intra_J25F2_t12_RD3_01_3086.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t12_RD4_01_3087 group:Rifa | ferm:F1 RAW_FILE_NAME=125_Intra_RifaF1_t12_RD4_01_3087.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF2_t2_RD5_01_3088 group:Rifa | ferm:F2 RAW_FILE_NAME=126_Intra_RifaF2_t2_RD5_01_3088.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t-1_RD6_01_3089 group:Stab | ferm:F1 RAW_FILE_NAME=127_Intra_SalmoF1_t-1_RD6_01_3089.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t10_RD7_01_3090 group:Salmo | ferm:F2 RAW_FILE_NAME=128_Intra_SalmoF2_t10_RD7_01_3090.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t0_RD8_01_3091 group:Salmo | ferm:F1 RAW_FILE_NAME=129_Intra_SalmoF1_t0_RD8_01_3091.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J27_RE1_01_3092 group:Stab | ferm:F2 RAW_FILE_NAME=130_Intra_StabF2_J27_RE1_01_3092.cdf; time=NA; time_stab=27 SUBJECT_SAMPLE_FACTORS - StabF1_J20_RE2_01_3093 group:Stab | ferm:F1 RAW_FILE_NAME=131_Intra_StabF1_J20_RE2_01_3093.cdf; time=NA; time_stab=20 SUBJECT_SAMPLE_FACTORS - RifaF2_t6_RE3_01_3096 group:Rifa | ferm:F2 RAW_FILE_NAME=132_Intra_RifaF2_t6_RE3_01_3096.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t0_RE4_01_3097 group:J25 | ferm:F1 RAW_FILE_NAME=133_Intra_J25F1_t0_RE4_01_3097.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t12_RE5_01_3098 group:Reut | ferm:F2 RAW_FILE_NAME=134_Intra_ReutF2_t12_RE5_01_3098.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF2_t8_RE6_01_3099 group:Rifa | ferm:F2 RAW_FILE_NAME=135_Intra_RifaF2_t8_RE6_01_3099.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t6_RE7_01_3100 group:Salmo | ferm:F2 RAW_FILE_NAME=136_Intra_SalmoF2_t6_RE7_01_3100.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t2_RE8_01_3101 group:Reut | ferm:F1 RAW_FILE_NAME=137_Intra_ReutF1_t2_RE8_01_3101.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t-1_BA1_01_3102 group:Stab | ferm:F1 RAW_FILE_NAME=138_Intra_SalmoF1_t-1_BA1_01_3102.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF2_t-1_BA2_01_3103 group:Stab | ferm:F2 RAW_FILE_NAME=139_Intra_RifaF2_t-1_BA2_01_3103.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t4_BA3_01_3104 group:Reut | ferm:F2 RAW_FILE_NAME=140_Intra_ReutF2_t4_BA3_01_3104.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t12_BA4_01_3105 group:Salmo | ferm:F2 RAW_FILE_NAME=141_Intra_SalmoF2_t12_BA4_01_3105.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J28_BA5_01_3106 group:Stab | ferm:F2 RAW_FILE_NAME=142_Intra_StabF2_J28_BA5_01_3106.cdf; time=NA; time_stab=28 SUBJECT_SAMPLE_FACTORS - J25F2_t6_BA6_01_3107 group:J25 | ferm:F2 RAW_FILE_NAME=143_Intra_J25F2_t6_BA6_01_3107.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t0_BA7_01_3108 group:Rifa | ferm:F1 RAW_FILE_NAME=144_Intra_RifaF1_t0_BA7_01_3108.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t2_BA8_01_3109 group:Rifa | ferm:F1 RAW_FILE_NAME=145_Intra_RifaF1_t2_BA8_01_3109.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF2_t12_BB1_01_3110 group:Rifa | ferm:F2 RAW_FILE_NAME=146_Intra_RifaF2_t12_BB1_01_3110.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t-1_BB2_01_3111 group:Stab | ferm:F1 RAW_FILE_NAME=147_Intra_ReutF1_t-1_BB2_01_3111.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t4_BB3_01_3114 group:Rifa | ferm:F1 RAW_FILE_NAME=148_Intra_RifaF1_t4_BB3_01_3114.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t10_BB4_01_3115 group:Salmo | ferm:F1 RAW_FILE_NAME=149_Intra_SalmoF1_t10_BB4_01_3115.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t-1_BB5_01_3116 group:Stab | ferm:F2 RAW_FILE_NAME=150_Intra_ReutF2_t-1_BB5_01_3116.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J28_BB6_01_3117 group:Stab | ferm:F1 RAW_FILE_NAME=151_Intra_StabF1_J28_BB6_01_3117.cdf; time=NA; time_stab=28 SUBJECT_SAMPLE_FACTORS - RifaF2_t6_BB7_01_3118 group:Rifa | ferm:F2 RAW_FILE_NAME=152_Intra_RifaF2_t6_BB7_01_3118.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t0_BB8_01_3119 group:Reut | ferm:F2 RAW_FILE_NAME=153_Intra_ReutF2_t0_BB8_01_3119.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t10_BC1_01_3120 group:Salmo | ferm:F2 RAW_FILE_NAME=154_Intra_SalmoF2_t10_BC1_01_3120.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t2_BC2_01_3121 group:Reut | ferm:F1 RAW_FILE_NAME=155_Intra_ReutF1_t2_BC2_01_3121.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t24_BC3_01_3122 group:J25 | ferm:F2 RAW_FILE_NAME=156_Intra_J25F2_t24_BC3_01_3122.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t0_BC4_01_3123 group:J25 | ferm:F1 RAW_FILE_NAME=157_Intra_J25F1_t0_BC4_01_3123.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t10_BC5_01_3124 group:J25 | ferm:F1 RAW_FILE_NAME=158_Intra_J25F1_t10_BC5_01_3124.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t2_BC6_01_3125 group:J25 | ferm:F1 RAW_FILE_NAME=159_Intra_J25F1_t2_BC6_01_3125.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J27_BC7_01_3126 group:Stab | ferm:F1 RAW_FILE_NAME=160_Intra_StabF1_J27_BC7_01_3126.cdf; time=NA; time_stab=27 SUBJECT_SAMPLE_FACTORS - J25F1_t6_BC8_01_3127 group:J25 | ferm:F1 RAW_FILE_NAME=161_Intra_J25F1_t6_BC8_01_3127.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t6_BD1_01_3128 group:Rifa | ferm:F1 RAW_FILE_NAME=162_Intra_RifaF1_t6_BD1_01_3128.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t-1_BD2_01_3129 group:Stab | ferm:F1 RAW_FILE_NAME=163_Intra_ReutF1_t-1_BD2_01_3129.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t4_BD3_01_3132 group:Reut | ferm:F2 RAW_FILE_NAME=164_Intra_ReutF2_t4_BD3_01_3132.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t2_BD4_01_3133 group:J25 | ferm:F1 RAW_FILE_NAME=165_Intra_J25F1_t2_BD4_01_3133.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t8_BD5_01_3134 group:J25 | ferm:F2 RAW_FILE_NAME=166_Intra_J25F2_t8_BD5_01_3134.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF2_t12_BD6_01_3135 group:Rifa | ferm:F2 RAW_FILE_NAME=167_Intra_RifaF2_t12_BD6_01_3135.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J22_BD7_01_3136 group:Stab | ferm:F1 RAW_FILE_NAME=168_Intra_StabF1_J22_BD7_01_3136.cdf; time=NA; time_stab=22 SUBJECT_SAMPLE_FACTORS - J25F1_t24_BD8_01_3137 group:J25 | ferm:F1 RAW_FILE_NAME=169_Intra_J25F1_t24_BD8_01_3137.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t8_BE1_01_3138 group:Rifa | ferm:F1 RAW_FILE_NAME=170_Intra_RifaF1_t8_BE1_01_3138.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J23_BE2_01_3139 group:Stab | ferm:F2 RAW_FILE_NAME=171_Intra_StabF2_J23_BE2_01_3139.cdf; time=NA; time_stab=23 SUBJECT_SAMPLE_FACTORS - RifaF2_t4_BE3_01_3140 group:Rifa | ferm:F2 RAW_FILE_NAME=172_Intra_RifaF2_t4_BE3_01_3140.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t24_BE4_01_3141 group:J25 | ferm:F2 RAW_FILE_NAME=173_Intra_J25F2_t24_BE4_01_3141.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J22_BE5_01_3142 group:Stab | ferm:F1 RAW_FILE_NAME=174_Intra_StabF1_J22_BE5_01_3142.cdf; time=NA; time_stab=22 SUBJECT_SAMPLE_FACTORS - ReutF1_t12_BE6_01_3143 group:Reut | ferm:F1 RAW_FILE_NAME=175_Intra_ReutF1_t12_BE6_01_3143.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t4_BE7_01_3144 group:Reut | ferm:F1 RAW_FILE_NAME=176_Intra_ReutF1_t4_BE7_01_3144.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J17_BE8_01_3145 group:Stab | ferm:F1 RAW_FILE_NAME=177_Intra_StabF1_J17_BE8_01_3145.cdf; time=NA; time_stab=17 SUBJECT_SAMPLE_FACTORS - StabF2_J16_GA1_01_3146 group:Stab | ferm:F2 RAW_FILE_NAME=178_Intra_StabF2_J16_GA1_01_3146.cdf; time=NA; time_stab=16 SUBJECT_SAMPLE_FACTORS - SalmoF2_t0_GA2_01_3147 group:Salmo | ferm:F2 RAW_FILE_NAME=179_Intra_SalmoF2_t0_GA2_01_3147.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t8_GA3_01_3150 group:Salmo | ferm:F1 RAW_FILE_NAME=180_Intra_SalmoF1_t8_GA3_01_3150.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF2_t24_GA4_01_3151 group:Rifa | ferm:F2 RAW_FILE_NAME=181_Intra_RifaF2_t24_GA4_01_3151.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t0_GA5_01_3152 group:J25 | ferm:F2 RAW_FILE_NAME=182_Intra_J25F2_t0_GA5_01_3152.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J17_GA6_01_3153 group:Stab | ferm:F2 RAW_FILE_NAME=183_Intra_StabF2_J17_GA6_01_3153.cdf; time=NA; time_stab=17 SUBJECT_SAMPLE_FACTORS - StabF1_J16_GA7_01_3154 group:Stab | ferm:F1 RAW_FILE_NAME=184_Intra_StabF1_J16_GA7_01_3154.cdf; time=NA; time_stab=16 SUBJECT_SAMPLE_FACTORS - SalmoF2_t4_GA8_01_3155 group:Salmo | ferm:F2 RAW_FILE_NAME=185_Intra_SalmoF2_t4_GA8_01_3155.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t8_GB1_01_3156 group:Salmo | ferm:F1 RAW_FILE_NAME=186_Intra_SalmoF1_t8_GB1_01_3156.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t8_GB2_01_3157 group:J25 | ferm:F1 RAW_FILE_NAME=187_Intra_J25F1_t8_GB2_01_3157.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t24_GB3_01_3158 group:Reut | ferm:F1 RAW_FILE_NAME=188_Intra_ReutF1_t24_GB3_01_3158.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t8_GB4_01_3159 group:Reut | ferm:F2 RAW_FILE_NAME=189_Intra_ReutF2_t8_GB4_01_3159.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_BA2_01_2960 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_BA2_01_2960.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_BA2_01_2962 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_BA2_01_2962.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_BA2_01_2972 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_BA2_01_2972.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_BA2_01_2982 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_BA2_01_2982.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_BA2_01_2992 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_BA2_01_2992.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_BA2_01_3002 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_BA2_01_3002.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_BA2_01_3012 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_BA2_01_3012.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_BA2_01_3022 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_BA2_01_3022.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_BA2_01_3040 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_BA2_01_3040.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_GE1_01_3058 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_GE1_01_3058.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_GE1_01_3076 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_GE1_01_3076.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_GE1_01_3094 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_GE1_01_3094.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_GE1_01_3112 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_GE1_01_3112.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_GE1_01_3130 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_GE1_01_3130.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - QC_GE1_01_3148 group:QC | ferm:QC RAW_FILE_NAME=19_Intra_QC_GE1_01_3148.cdf; time=NA; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t24_GB5_01_3160 group:Rifa | ferm:F1 RAW_FILE_NAME=190_Intra_RifaF1_t24_GB5_01_3160.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t-1_GB6_01_3161 group:Stab | ferm:F1 RAW_FILE_NAME=191_Intra_J25F1_t-1_GB6_01_3161.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t24_GB7_01_3162 group:Salmo | ferm:F2 RAW_FILE_NAME=192_Intra_SalmoF2_t24_GB7_01_3162.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t0_GB8_01_3163 group:Reut | ferm:F2 RAW_FILE_NAME=193_Intra_ReutF2_t0_GB8_01_3163.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t6_GC1_01_3164 group:Reut | ferm:F1 RAW_FILE_NAME=194_Intra_ReutF1_t6_GC1_01_3164.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t4_GC2_01_3165 group:Rifa | ferm:F1 RAW_FILE_NAME=195_Intra_RifaF1_t4_GC2_01_3165.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t6_GC3_01_3166 group:J25 | ferm:F2 RAW_FILE_NAME=196_Intra_J25F2_t6_GC3_01_3166.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t24_GC4_01_3167 group:Reut | ferm:F2 RAW_FILE_NAME=197_Intra_ReutF2_t24_GC4_01_3167.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J22_GC5_01_3168 group:Stab | ferm:F2 RAW_FILE_NAME=198_Intra_StabF2_J22_GC5_01_3168.cdf; time=NA; time_stab=22 SUBJECT_SAMPLE_FACTORS - J25F2_t4_GC6_01_3169 group:J25 | ferm:F2 RAW_FILE_NAME=199_Intra_J25F2_t4_GC6_01_3169.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabFM_J9_GC7_01_3170 group:Stab | ferm:FM RAW_FILE_NAME=200_Intra_StabFM_J9_GC7_01_3170.cdf; time=NA; time_stab=9 SUBJECT_SAMPLE_FACTORS - RifaF2_t0_GC8_01_3171 group:Rifa | ferm:F2 RAW_FILE_NAME=201_Intra_RifaF2_t0_GC8_01_3171.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J20_BA3_01_2964 group:Stab | ferm:F2 RAW_FILE_NAME=21_Intra_StabF2_J20_BA3_01_2964.cdf; time=NA; time_stab=20 SUBJECT_SAMPLE_FACTORS - RifaF2_t4_BA4_01_2965 group:Rifa | ferm:F2 RAW_FILE_NAME=22_Intra_RifaF2_t4_BA4_01_2965.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t4_BA5_01_2966 group:Reut | ferm:F1 RAW_FILE_NAME=23_Intra_ReutF1_t4_BA5_01_2966.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t4_BA6_01_2967 group:Salmo | ferm:F2 RAW_FILE_NAME=24_Intra_SalmoF2_t4_BA6_01_2967.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t4_BA7_01_2968 group:J25 | ferm:F1 RAW_FILE_NAME=25_Intra_J25F1_t4_BA7_01_2968.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J20_BA8_01_2969 group:Stab | ferm:F1 RAW_FILE_NAME=26_Intra_StabF1_J20_BA8_01_2969.cdf; time=NA; time_stab=20 SUBJECT_SAMPLE_FACTORS - StabFM_J12_BB1_01_2970 group:Stab | ferm:FM RAW_FILE_NAME=27_Intra_StabFM_J12_BB1_01_2970.cdf; time=NA; time_stab=12 SUBJECT_SAMPLE_FACTORS - J25F1_t12_BB2_01_2971 group:J25 | ferm:F1 RAW_FILE_NAME=28_Intra_J25F1_t12_BB2_01_2971.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabFM_J16_BB3_01_2974 group:Stab | ferm:FM RAW_FILE_NAME=29_Intra_StabFM_J16_BB3_01_2974.cdf; time=NA; time_stab=16 SUBJECT_SAMPLE_FACTORS - SalmoF2_t0_BB4_01_2975 group:Salmo | ferm:F2 RAW_FILE_NAME=30_Intra_SalmoF2_t0_BB4_01_2975.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t12_BB5_01_2976 group:Reut | ferm:F1 RAW_FILE_NAME=31_Intra_ReutF1_t12_BB5_01_2976.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t8_BB6_01_2977 group:Salmo | ferm:F2 RAW_FILE_NAME=32_Intra_SalmoF2_t8_BB6_01_2977.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t8_BB7_01_2978 group:Rifa | ferm:F1 RAW_FILE_NAME=33_Intra_RifaF1_t8_BB7_01_2978.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t8_BB8_01_2979 group:J25 | ferm:F2 RAW_FILE_NAME=34_Intra_J25F2_t8_BB8_01_2979.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J22_BC1_01_2980 group:Stab | ferm:F2 RAW_FILE_NAME=35_Intra_StabF2_J22_BC1_01_2980.cdf; time=NA; time_stab=22 SUBJECT_SAMPLE_FACTORS - SalmoF1_t12_BC2_01_2981 group:Salmo | ferm:F1 RAW_FILE_NAME=36_Intra_SalmoF1_t12_BC2_01_2981.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF2_t-1_BC3_01_2984 group:Stab | ferm:F2 RAW_FILE_NAME=37_Intra_RifaF2_t-1_BC3_01_2984.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J28_BC4_01_2985 group:Stab | ferm:F2 RAW_FILE_NAME=38_Intra_StabF2_J28_BC4_01_2985.cdf; time=NA; time_stab=28 SUBJECT_SAMPLE_FACTORS - J25F1_t8_BC5_01_2986 group:J25 | ferm:F1 RAW_FILE_NAME=39_Intra_J25F1_t8_BC5_01_2986.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t-1_BC6_01_2987 group:Stab | ferm:F1 RAW_FILE_NAME=40_Intra_RifaF1_t-1_BC6_01_2987.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t4_BC7_01_2988 group:Salmo | ferm:F1 RAW_FILE_NAME=41_Intra_SalmoF1_t4_BC7_01_2988.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF2_t8_BC8_01_2989 group:Rifa | ferm:F2 RAW_FILE_NAME=42_Intra_RifaF2_t8_BC8_01_2989.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t24_BD1_01_2990 group:Rifa | ferm:F1 RAW_FILE_NAME=43_Intra_RifaF1_t24_BD1_01_2990.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t-1_BD2_01_2991 group:Stab | ferm:F2 RAW_FILE_NAME=44_Intra_ReutF2_t-1_BD2_01_2991.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabFM_J10_BD3_01_2994 group:Stab | ferm:FM RAW_FILE_NAME=45_Intra_StabFM_J10_BD3_01_2994.cdf; time=NA; time_stab=10 SUBJECT_SAMPLE_FACTORS - SalmoF2_t-1_BD4_01_2995 group:Stab | ferm:F2 RAW_FILE_NAME=46_Intra_SalmoF2_t-1_BD4_01_2995.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabFM_J9_BD5_01_2996 group:Stab | ferm:FM RAW_FILE_NAME=47_Intra_StabFM_J9_BD5_01_2996.cdf; time=NA; time_stab=9 SUBJECT_SAMPLE_FACTORS - RifaF1_t12_BD6_01_2997 group:Rifa | ferm:F1 RAW_FILE_NAME=48_Intra_RifaF1_t12_BD6_01_2997.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t10_BD7_01_2998 group:J25 | ferm:F2 RAW_FILE_NAME=49_Intra_J25F2_t10_BD7_01_2998.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J23_BD8_01_2999 group:Stab | ferm:F1 RAW_FILE_NAME=50_Intra_StabF1_J23_BD8_01_2999.cdf; time=NA; time_stab=23 SUBJECT_SAMPLE_FACTORS - StabF2_J19_BE1_01_3000 group:Stab | ferm:F2 RAW_FILE_NAME=51_Intra_StabF2_J19_BE1_01_3000.cdf; time=NA; time_stab=19 SUBJECT_SAMPLE_FACTORS - SalmoF1_t12_BE2_01_3001 group:Salmo | ferm:F1 RAW_FILE_NAME=52_Intra_SalmoF1_t12_BE2_01_3001.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t-1_BE3_01_3004 group:Stab | ferm:F2 RAW_FILE_NAME=53_Intra_SalmoF2_t-1_BE3_01_3004.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabFM_J16_BE4_01_3005 group:Stab | ferm:FM RAW_FILE_NAME=54_Intra_StabFM_J16_BE4_01_3005.cdf; time=NA; time_stab=16 SUBJECT_SAMPLE_FACTORS - J25F2_t10_BE5_01_3006 group:J25 | ferm:F2 RAW_FILE_NAME=55_Intra_J25F2_t10_BE5_01_3006.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t-1_BE6_01_3007 group:Stab | ferm:F1 RAW_FILE_NAME=56_Intra_J25F1_t-1_BE6_01_3007.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t2_BE7_01_3008 group:Salmo | ferm:F2 RAW_FILE_NAME=57_Intra_SalmoF2_t2_BE7_01_3008.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J23_BE8_01_3009 group:Stab | ferm:F1 RAW_FILE_NAME=58_Intra_StabF1_J23_BE8_01_3009.cdf; time=NA; time_stab=23 SUBJECT_SAMPLE_FACTORS - ReutF2_t2_GA1_01_3010 group:Reut | ferm:F2 RAW_FILE_NAME=59_Intra_ReutF2_t2_GA1_01_3010.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabFM_J12_GA2_01_3011 group:Stab | ferm:FM RAW_FILE_NAME=60_Intra_StabFM_J12_GA2_01_3011.cdf; time=NA; time_stab=12 SUBJECT_SAMPLE_FACTORS - J25F2_t-1_GA3_01_3014 group:Stab | ferm:F2 RAW_FILE_NAME=61_Intra_J25F2_t-1_GA3_01_3014.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t12_GA4_01_3015 group:Salmo | ferm:F2 RAW_FILE_NAME=62_Intra_SalmoF2_t12_GA4_01_3015.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabFM_J10_GA5_01_3016 group:Stab | ferm:FM RAW_FILE_NAME=63_Intra_StabFM_J10_GA5_01_3016.cdf; time=NA; time_stab=10 SUBJECT_SAMPLE_FACTORS - SalmoF1_t6_GA6_01_3017 group:Salmo | ferm:F1 RAW_FILE_NAME=64_Intra_SalmoF1_t6_GA6_01_3017.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J27_GA7_01_3018 group:Stab | ferm:F1 RAW_FILE_NAME=65_Intra_StabF1_J27_GA7_01_3018.cdf; time=NA; time_stab=27 SUBJECT_SAMPLE_FACTORS - RifaF2_t10_GA8_01_3019 group:Rifa | ferm:F2 RAW_FILE_NAME=66_Intra_RifaF2_t10_GA8_01_3019.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t2_GB1_01_3020 group:J25 | ferm:F2 RAW_FILE_NAME=67_Intra_J25F2_t2_GB1_01_3020.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t6_GB2_01_3021 group:Reut | ferm:F1 RAW_FILE_NAME=68_Intra_ReutF1_t6_GB2_01_3021.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t2_GB3_01_3024 group:J25 | ferm:F2 RAW_FILE_NAME=69_Intra_J25F2_t2_GB3_01_3024.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t10_GB4_01_3025 group:Salmo | ferm:F1 RAW_FILE_NAME=70_Intra_SalmoF1_t10_GB4_01_3025.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t12_GB5_01_3026 group:Reut | ferm:F2 RAW_FILE_NAME=71_Intra_ReutF2_t12_GB5_01_3026.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J16_GB6_01_3027 group:Stab | ferm:F2 RAW_FILE_NAME=72_Intra_StabF2_J16_GB6_01_3027.cdf; time=NA; time_stab=16 SUBJECT_SAMPLE_FACTORS - ReutF1_t10_GB7_01_3028 group:Reut | ferm:F1 RAW_FILE_NAME=73_Intra_ReutF1_t10_GB7_01_3028.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t10_GB8_01_3029 group:J25 | ferm:F1 RAW_FILE_NAME=74_Intra_J25F1_t10_GB8_01_3029.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t2_GC1_01_3030 group:Salmo | ferm:F1 RAW_FILE_NAME=75_Intra_SalmoF1_t2_GC1_01_3030.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t-1_GC2_01_3031 group:Stab | ferm:F2 RAW_FILE_NAME=76_Intra_J25F2_t-1_GC2_01_3031.cdf; time=-1; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF2_t0_GC3_01_3032 group:Rifa | ferm:F2 RAW_FILE_NAME=77_Intra_RifaF2_t0_GC3_01_3032.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t8_GC4_01_3033 group:Reut | ferm:F1 RAW_FILE_NAME=78_Intra_ReutF1_t8_GC4_01_3033.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF2_t10_GC5_01_3034 group:Rifa | ferm:F2 RAW_FILE_NAME=79_Intra_RifaF2_t10_GC5_01_3034.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t2_GC6_01_3035 group:Salmo | ferm:F1 RAW_FILE_NAME=80_Intra_SalmoF1_t2_GC6_01_3035.cdf; time=2; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t12_GC7_01_3036 group:J25 | ferm:F1 RAW_FILE_NAME=81_Intra_J25F1_t12_GC7_01_3036.cdf; time=12; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J17_GC8_01_3037 group:Stab | ferm:F2 RAW_FILE_NAME=82_Intra_StabF2_J17_GC8_01_3037.cdf; time=NA; time_stab=17 SUBJECT_SAMPLE_FACTORS - SalmoF1_t0_GD1_01_3038 group:Salmo | ferm:F1 RAW_FILE_NAME=83_Intra_SalmoF1_t0_GD1_01_3038.cdf; time=-; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F1_t6_GD2_01_3039 group:J25 | ferm:F1 RAW_FILE_NAME=84_Intra_J25F1_t6_GD2_01_3039.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF1_t24_GD3_01_3042 group:Reut | ferm:F1 RAW_FILE_NAME=85_Intra_ReutF1_t24_GD3_01_3042.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF2_t4_GD4_01_3043 group:Salmo | ferm:F2 RAW_FILE_NAME=86_Intra_SalmoF2_t4_GD4_01_3043.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J20_GD5_01_3044 group:Stab | ferm:F2 RAW_FILE_NAME=87_Intra_StabF2_J20_GD5_01_3044.cdf; time=NA; time_stab=20 SUBJECT_SAMPLE_FACTORS - SalmoF1_t24_GD6_01_3045 group:Salmo | ferm:F1 RAW_FILE_NAME=88_Intra_SalmoF1_t24_GD6_01_3045.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t10_GD7_01_3046 group:Rifa | ferm:F1 RAW_FILE_NAME=89_Intra_RifaF1_t10_GD7_01_3046.cdf; time=10; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J28_GD8_01_3047 group:Stab | ferm:F1 RAW_FILE_NAME=90_Intra_StabF1_J28_GD8_01_3047.cdf; time=NA; time_stab=28 SUBJECT_SAMPLE_FACTORS - SalmoF1_t24_GE1_01_3048 group:Salmo | ferm:F1 RAW_FILE_NAME=91_Intra_SalmoF1_t24_GE1_01_3048.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t24_GE2_01_3049 group:Reut | ferm:F2 RAW_FILE_NAME=92_Intra_ReutF2_t24_GE2_01_3049.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - RifaF1_t6_GE3_01_3050 group:Rifa | ferm:F1 RAW_FILE_NAME=93_Intra_RifaF1_t6_GE3_01_3050.cdf; time=6; time_stab=NA SUBJECT_SAMPLE_FACTORS - J25F2_t4_GE4_01_3051 group:J25 | ferm:F2 RAW_FILE_NAME=94_Intra_J25F2_t4_GE4_01_3051.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF1_J17_GE5_01_3052 group:Stab | ferm:F1 RAW_FILE_NAME=95_Intra_StabF1_J17_GE5_01_3052.cdf; time=NA; time_stab=17 SUBJECT_SAMPLE_FACTORS - RifaF2_t24_GE6_01_3053 group:Rifa | ferm:F2 RAW_FILE_NAME=96_Intra_RifaF2_t24_GE6_01_3053.cdf; time=24; time_stab=NA SUBJECT_SAMPLE_FACTORS - SalmoF1_t4_GE7_01_3054 group:Salmo | ferm:F1 RAW_FILE_NAME=97_Intra_SalmoF1_t4_GE7_01_3054.cdf; time=4; time_stab=NA SUBJECT_SAMPLE_FACTORS - ReutF2_t8_GE8_01_3055 group:Reut | ferm:F2 RAW_FILE_NAME=98_Intra_ReutF2_t8_GE8_01_3055.cdf; time=8; time_stab=NA SUBJECT_SAMPLE_FACTORS - StabF2_J19_RA1_01_3056 group:Stab | ferm:F2 RAW_FILE_NAME=99_Intra_StabF2_J19_RA1_01_3056.cdf; time=NA; time_stab=19 #COLLECTION CO:COLLECTION_SUMMARY The PolyFermS in vitro continuous fermentation model was used here with modified CO:COLLECTION_SUMMARY Macfarlane medium to simulate the porcine proximal colon. During 35 days of CO:COLLECTION_SUMMARY fermentation, a first-stage reactor containing immobilized swine fecal CO:COLLECTION_SUMMARY microbiota fed two second-stage control and test reactors operated in parallel CO:COLLECTION_SUMMARY and used to test the effects of MccJ25 on the metabolic activity of the CO:COLLECTION_SUMMARY microbiota. CO:SAMPLE_TYPE Bacterial cells CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY Performed simultaneously in TR1 and TR2, the four treatments consisted of adding TR:TREATMENT_SUMMARY by adding Salmonella enterica subsp. enterica serovar Newport ATCC 6962 alone at TR:TREATMENT_SUMMARY an initial concentration of 107 cfu/mL to each reactor, followed by adding the TR:TREATMENT_SUMMARY bacteria at this cell density along with each tested antimicrobial: 0.4 mM TR:TREATMENT_SUMMARY MccJ25, 4 mM reuterin or 0.6 mM rifampicin. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The metabolites were extracted from swine colonic microbiota in SP:SAMPLEPREP_SUMMARY methanol/chloroform/water. The aqueous phase was collected. SP:PROCESSING_STORAGE_CONDITIONS On ice SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Low pH polar CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 RS CH:COLUMN_NAME Acclaim Polar Advantage II column (Thermo) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Bruker Maxis II ETD MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Proprietary analytical software for MS acquisition Data processing with xcms (R MS:MS_COMMENTS package) MS:MS_RESULTS_FILE ST002014_AN003282_Results.txt UNITS:m/z Has m/z:Yes Has RT:Yes RT units:Minutes #END