#METABOLOMICS WORKBENCH lgmartins1984_20220331_114038 DATATRACK_ID:3151 STUDY_ID:ST002133 ANALYSIS_ID:AN003488 PROJECT_ID:PR001351 VERSION 1 CREATED_ON March 31, 2022, 3:40 pm #PROJECT PR:PROJECT_TITLE Untarget metabolomics of HIV patients carrier PR:PROJECT_TYPE NMR-based metabolomics PR:PROJECT_SUMMARY Analysis of metabolite variation in HIV patients carrier blood serum in response PR:PROJECT_SUMMARY to treatment with effervencent glutamine. PR:INSTITUTE University of Campinas PR:DEPARTMENT Organic Chemistry PR:LABORATORY Biological Chemistry Lab PR:LAST_NAME Martins PR:FIRST_NAME Lucas PR:ADDRESS Cidade Universitário Zeferino Vaz, Campinas, SP, Brazil PR:EMAIL lgmartins1984@gmail.com PR:PHONE +5519992947044 PR:FUNDING_SOURCE FAPESP, INCT-Bio #STUDY ST:STUDY_TITLE Metabolite variation in the blood serum of HIV patients carrier treated with ST:STUDY_TITLE effervecent glutamine ST:STUDY_TYPE Liquid NMR experiments ST:STUDY_SUMMARY It was demonstrated that effervescent glutamine supplementation in people living ST:STUDY_SUMMARY with HIV/AIDS increased the amount of CD4+ T lymphocytes. , decreased the serum ST:STUDY_SUMMARY levels of biomarkers of inflammation, and introduced health benefits. Herein, ST:STUDY_SUMMARY NMR spectroscopy was applied to evaluate if oral ingestion of glutamine (12.5 g) ST:STUDY_SUMMARY for 30 days can be measured through serum metabolite variations and if the ST:STUDY_SUMMARY reported benefits might correlate to small metabolites' changes in a chosen ST:STUDY_SUMMARY cohort of individuals. The HIV/AIDS group has been carefully selected and ST:STUDY_SUMMARY studied before and after the 30-days of supplementation with the glutamine, ST:STUDY_SUMMARY together with the matched non-HIV carrier patients' group. The group of HIV/AIDS ST:STUDY_SUMMARY individuals presented lower (p < 0.05) levels of choline, creatine, pyruvate, ST:STUDY_SUMMARY glutamate, lysine, and tyrosine when compared to the non-HIV carrier patients. ST:STUDY_SUMMARY On the other hand, serum concentrations of glucose, lipids, lactate, glutamine, ST:STUDY_SUMMARY phenylalanine, and threonine were higher (p < 0.05) in HIV/AIDS individuals. The ST:STUDY_SUMMARY variation in metabolites as a result of treatment with glutamine is consistent ST:STUDY_SUMMARY with the improvements observed in these patients and may lead us to suggest the ST:STUDY_SUMMARY introduction of the effervescent glutamine supplementation to antiretroviral ST:STUDY_SUMMARY therapies in people living with HIV/AIDS. ST:INSTITUTE University of Campinas ST:DEPARTMENT Organic Chemistry ST:LABORATORY Biological Chemistry Lab ST:LAST_NAME MARTINS ST:FIRST_NAME LUCAS ST:ADDRESS Rua Josué de Castro, s/n - Cidade Universitária, Campinas - SP, 13083-970 ST:EMAIL lgmartins1984@gmail.com ST:PHONE +5519992947044 ST:NUM_GROUPS 3 ST:TOTAL_SUBJECTS 24 ST:NUM_MALES 10 ST:NUM_FEMALES 14 ST:STUDY_COMMENTS Crossover study #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:AGE_OR_AGE_RANGE 40.3 ± 3.9 SU:HUMAN_NUTRITION Effervecent glutamine administration (12.5g, once a day for 30 days) SU:HUMAN_INCLUSION_CRITERIA Patients living with HIV from Public Service Health Care for Sexually SU:HUMAN_INCLUSION_CRITERIA Transmitted Diseases/AIDS Center of Maringá City, Paraná, Brazil, with same SU:HUMAN_INCLUSION_CRITERIA ART treatment for at least 1 year. SU:HUMAN_EXCLUSION_CRITERIA Patients which were transferred, who abandoned treatment, and those who had not SU:HUMAN_EXCLUSION_CRITERIA been evaluated for viral load and CD4+ T lymphocyte counts were excluded. #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 1pre1 Sample class:BT RAW_FILE_NAME=1pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 2pre1 Sample class:BT RAW_FILE_NAME=2pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 4pre1 Sample class:BT RAW_FILE_NAME=4pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 6pre1 Sample class:BT RAW_FILE_NAME=6pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 8pre1 Sample class:BT RAW_FILE_NAME=8pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 9pre1 Sample class:BT RAW_FILE_NAME=9pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 10pre1 Sample class:BT RAW_FILE_NAME=10pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 12pre1 Sample class:BT RAW_FILE_NAME=12pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 13pre1 Sample class:BT RAW_FILE_NAME=13pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 16pre1 Sample class:BT RAW_FILE_NAME=16pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 14pre1 Sample class:BT RAW_FILE_NAME=14pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 17pre1 Sample class:BT RAW_FILE_NAME=17pre1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 2pre2 Sample class:BT RAW_FILE_NAME=2pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 4pre2 Sample class:BT RAW_FILE_NAME=4pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 6pre2 Sample class:BT RAW_FILE_NAME=6pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 8pre2 Sample class:BT RAW_FILE_NAME=8pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 9pre2 Sample class:BT RAW_FILE_NAME=9pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 10pre2 Sample class:BT RAW_FILE_NAME=10pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 12pre2 Sample class:BT RAW_FILE_NAME=12pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 13pre2 Sample class:BT RAW_FILE_NAME=13pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 14pre2 Sample class:BT RAW_FILE_NAME=14pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 15pre2 Sample class:BT RAW_FILE_NAME=15pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 16pre2 Sample class:BT RAW_FILE_NAME=16pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 17pre2 Sample class:BT RAW_FILE_NAME=17pre2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 1pos1 Sample class:AT RAW_FILE_NAME=1pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 2pos1 Sample class:AT RAW_FILE_NAME=2pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 4pos1 Sample class:AT RAW_FILE_NAME=4pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 6pos1 Sample class:AT RAW_FILE_NAME=6pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 8pos1 Sample class:AT RAW_FILE_NAME=8pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 9pos1 Sample class:AT RAW_FILE_NAME=9pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 10pos1 Sample class:AT RAW_FILE_NAME=10pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 12pos1 Sample class:AT RAW_FILE_NAME=12pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 13pos1 Sample class:AT RAW_FILE_NAME=13pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 14pos1 Sample class:AT RAW_FILE_NAME=14pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 15pos1 Sample class:AT RAW_FILE_NAME=15pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 17pos1 Sample class:AT RAW_FILE_NAME=17pos1; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 1pos2 Sample class:AT RAW_FILE_NAME=1pos2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 4pos2 Sample class:AT RAW_FILE_NAME=4pos2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 6pos2 Sample class:AT RAW_FILE_NAME=6pos2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 8pos2 Sample class:AT RAW_FILE_NAME=8pos2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 10pos2 Sample class:AT RAW_FILE_NAME=10pos2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 12pos2 Sample class:AT RAW_FILE_NAME=12pos2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 13pos2 Sample class:AT RAW_FILE_NAME=13pos2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 14pos2 Sample class:AT RAW_FILE_NAME=14pos2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 15pos2 Sample class:AT RAW_FILE_NAME=15pos2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 16pos2 Sample class:AT RAW_FILE_NAME=16pos2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG SUBJECT_SAMPLE_FACTORS - 17pos2 Sample class:AT RAW_FILE_NAME=17pos2; NMR sample ID=3; Sample description=Blood serum; NMR experiment=1H CPMG #COLLECTION CO:COLLECTION_SUMMARY Serum samples were obtained from 12 HIV/AIDS patients before (day 0) and after CO:COLLECTION_SUMMARY glutamine (day 31) administration, and from healthy donors. CO:SAMPLE_TYPE Blood (serum) CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY Glutamine supplementation (12.5g/day for 30 days) #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Serum samples were carefully separated and aliquoted in duplicates (250 μL each SP:SAMPLEPREP_SUMMARY one) into cryovials and stored at -80 °C. Samples (250 μL) were thawed on ice, SP:SAMPLEPREP_SUMMARY mixed with 250 μL of deuterium oxide (D2O 99.9%, Cambridge Isotope SP:SAMPLEPREP_SUMMARY Laboratories, Inc., Andover, USA) at room temperature, centrifuged at 12,000 × SP:SAMPLEPREP_SUMMARY g for 2 min at 4 °C, and transferred into 5 mm NMR tubes SP:PROCESSING_STORAGE_CONDITIONS -80℃ #ANALYSIS AN:ANALYSIS_TYPE NMR #NMR NM:INSTRUMENT_NAME Avance 600 Bruker NM:INSTRUMENT_TYPE FT-NMR NM:NMR_EXPERIMENT_TYPE 1D-1H NM:SPECTROMETER_FREQUENCY 600 MHz for 1H NM:NMR_PROBE inverse triple-core probe (TBI) NM:NMR_SOLVENT H2O/D2O NM:NMR_TUBE_SIZE 5 mm NM:NMR_RESULTS_FILE ST002133_AN003488_Results.txt UNITS:intensity #END