#METABOLOMICS WORKBENCH Andre_Gollowitzer_20221012_053700 DATATRACK_ID:3504 STUDY_ID:ST002326 ANALYSIS_ID:AN003795 PROJECT_ID:PR001490 VERSION 1 CREATED_ON October 26, 2022, 6:28 pm #PROJECT PR:PROJECT_TITLE Reducing the metabolic burden of rRNA synthesis promotes healthy longevity PR:PROJECT_SUMMARY Ribosome biogenesis is an anabolic process driven by RNA polymerase I (Pol PR:PROJECT_SUMMARY I)-mediated synthesis of pre-ribosomal RNA (pre-rRNA). While Pol I activity was PR:PROJECT_SUMMARY previously linked to longevity, the underlying mechanisms were not studied PR:PROJECT_SUMMARY beyond effects on protein translation and downstream proteostasis. Here we used PR:PROJECT_SUMMARY multi-omics and functional tests to show that curtailment of Pol I activity PR:PROJECT_SUMMARY preserves mitochondrial function and lowers ATP expenditure, thereby promoting PR:PROJECT_SUMMARY longevity in Caenorhabditis elegans. Reduced pre-rRNA synthesis also improved PR:PROJECT_SUMMARY longevity and energy homeostasis in Drosophila melanogaster and human cells, PR:PROJECT_SUMMARY respectively. Conversely, the enhancement of pre-rRNA synthesis boosted growth PR:PROJECT_SUMMARY and neuromuscular performance of young nematodes at the cost of accelerated PR:PROJECT_SUMMARY metabolic decline, mitochondrial stress and premature aging. Moreover, PR:PROJECT_SUMMARY restriction of Pol I activity extended lifespan more potently than translational PR:PROJECT_SUMMARY repression, and retained its geroprotective effects when initiated late in life, PR:PROJECT_SUMMARY showcasing moderation of Pol I activity as an effective longevity treatment not PR:PROJECT_SUMMARY limited by aging. PR:INSTITUTE University of Innsbruck PR:DEPARTMENT Michael Popp Institute PR:LAST_NAME Koeberle PR:FIRST_NAME Andreas PR:ADDRESS Mitterweg 24, Innsbruck, Tyrol, 6020, Austria PR:EMAIL andreas.koeberle@uibk.ac.at PR:PHONE +43 512 507 57903 PR:FUNDING_SOURCE Leibniz Association, Thüringer Aufbaubank (2019 FGR 0082), Free State of PR:FUNDING_SOURCE Thuringia (RegenerAging—FSU-I-03/14), Carl-Zeiss-Stiftung (IMPULS), PR:FUNDING_SOURCE Phospholipid Research Center (AKO-2019-070/2-1) PR:CONTRIBUTORS Samim Sharifi, Finja Witt, André Gollowitzer, Oliver Werz, Holger Bierhoff, PR:CONTRIBUTORS Maria Ermolaeva #STUDY ST:STUDY_TITLE Analysis of age-dependent changes of triacylglycerol species in C. elegans ST:STUDY_TITLE depleted of tif-IA and ncl-1 ST:STUDY_SUMMARY Analysis of triacylglycerol species in young (d2), middle age (d6) and old age ST:STUDY_SUMMARY (d12) C. elegans with tif-IA or ncl-1 knockdown. ST:INSTITUTE University of Innsbruck ST:LAST_NAME Koeberle ST:FIRST_NAME Andreas ST:ADDRESS Mitterweg 24, Innsbruck, Tyrol, 6020, Austria ST:EMAIL andreas.koeberle@uibk.ac.at ST:PHONE +43 512 507 57903 #SUBJECT SU:SUBJECT_TYPE Invertebrate SU:SUBJECT_SPECIES Caenorhabditis elegans SU:TAXONOMY_ID 6239 SU:AGE_OR_AGE_RANGE Young, middle aged and old #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva001 Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva002 Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva003 Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva004 Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva005 Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva006 Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva007 Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva008 Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva009 Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva010 Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva011 Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva012 Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva013 Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva014 Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva015 Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva016 Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva017 Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva018 Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva019 Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva020 Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva021 Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva022 Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva023 Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva024 Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva025 Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva026 Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva027 Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva028 Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva029 Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva030 Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva031 Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva032 Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva033 Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva034 Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva035 Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva036 Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva037 Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva038 Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva039 Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva040 Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva041 Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva042 Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva043 Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva044 Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva045 Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGI_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva001_TAGII Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva002_TAGII Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva003_TAGII Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva004_TAGII Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva005_TAGII Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva006_TAGII Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva007_TAGII Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva008_TAGII Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva009_TAGII Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva010_TAGII Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva011_TAGII Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva012_TAGII Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva013_TAGII Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva014_TAGII Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva015_TAGII Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva016_TAGII Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva017_TAGII Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva018_TAGII Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva019_TAGII Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva020_TAGII Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva021_TAGII Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva022_TAGII Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva023_TAGII Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva024_TAGII Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva025_TAGII Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva026_TAGII Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva027_TAGII Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva028_TAGII Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva029_TAGII Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva030_TAGII Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva031_TAGII Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva032_TAGII Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva033_TAGII Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva034_TAGII Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva035_TAGII Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva036_TAGII Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva037_TAGII Treatment:ctrl RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva038_TAGII Treatment:ctrl RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva039_TAGII Treatment:ctrl RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva040_TAGII Treatment:tif-IA RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva041_TAGII Treatment:tif-IA RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva042_TAGII Treatment:tif-IA RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva043_TAGII Treatment:ncl-1 RNAi | Days of adulthood:2 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva044_TAGII Treatment:ncl-1 RNAi | Days of adulthood:6 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff SUBJECT_SAMPLE_FACTORS - 191111_FW_1_project_Ermolaeva045_TAGII Treatment:ncl-1 RNAi | Days of adulthood:12 RAW_FILE_NAME=191111_FW_1_project_Ermolaeva_TAGII_1_10.wiff #COLLECTION CO:COLLECTION_SUMMARY Worms were washed and pelleted, M9 media was removed with ~100µl remaining in CO:COLLECTION_SUMMARY the sample, and the sample was snap frozen. CO:SAMPLE_TYPE Worms #TREATMENT TR:TREATMENT_SUMMARY Ncl-1 and tif-IA knockdown was achieved by feeding the worms for 2 generations TR:TREATMENT_SUMMARY with bacteria (from the Ahringer RNAi feeding library, Source BioScience Ltd.) TR:TREATMENT_SUMMARY producing the corresponding dsRNA. Worms were cultured under standard conditions TR:TREATMENT_SUMMARY as described in ‘Wormbook’, F1 was grown at 15°C and F2 at 20°C, and F2 TR:TREATMENT_SUMMARY animals were grown on RNAi until days 2, 6 or 12 of adulthood. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Triacylglycerols were extracted from C. elegans by successive addition of PBS pH SP:SAMPLEPREP_SUMMARY 7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17. SP:SAMPLEPREP_SUMMARY Evaporation of the organic layer yielded a lipid film that was dissolved in SP:SAMPLEPREP_SUMMARY methanol and subjected to UPLC-MS/MS. SP:EXTRACT_STORAGE -20℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Chromatographic separation of triacylglycerols was carried out on an Acquity BEH CH:CHROMATOGRAPHY_SUMMARY C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an Acquity UHPLC. CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity H-Class CH:COLUMN_NAME Waters Acquity BEH C8 (100 x 2.1mm, 1.7um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 5500 QTrap MS:INSTRUMENT_TYPE QTRAP MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Targeted MRM with pre-optimized settings and subsequent automated integration of MS:MS_COMMENTS selected signals using Analyst 1.6.2 or Analyst 1.6.3 (Sciex). #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS relative units MS_METABOLITE_DATA_START Samples 191111_FW_1_project_Ermolaeva001 191111_FW_1_project_Ermolaeva002 191111_FW_1_project_Ermolaeva003 191111_FW_1_project_Ermolaeva004 191111_FW_1_project_Ermolaeva005 191111_FW_1_project_Ermolaeva006 191111_FW_1_project_Ermolaeva007 191111_FW_1_project_Ermolaeva008 191111_FW_1_project_Ermolaeva009 191111_FW_1_project_Ermolaeva010 191111_FW_1_project_Ermolaeva011 191111_FW_1_project_Ermolaeva012 191111_FW_1_project_Ermolaeva013 191111_FW_1_project_Ermolaeva014 191111_FW_1_project_Ermolaeva015 191111_FW_1_project_Ermolaeva016 191111_FW_1_project_Ermolaeva017 191111_FW_1_project_Ermolaeva018 191111_FW_1_project_Ermolaeva019 191111_FW_1_project_Ermolaeva020 191111_FW_1_project_Ermolaeva021 191111_FW_1_project_Ermolaeva022 191111_FW_1_project_Ermolaeva023 191111_FW_1_project_Ermolaeva024 191111_FW_1_project_Ermolaeva025 191111_FW_1_project_Ermolaeva026 191111_FW_1_project_Ermolaeva027 191111_FW_1_project_Ermolaeva028 191111_FW_1_project_Ermolaeva029 191111_FW_1_project_Ermolaeva030 191111_FW_1_project_Ermolaeva031 191111_FW_1_project_Ermolaeva032 191111_FW_1_project_Ermolaeva033 191111_FW_1_project_Ermolaeva034 191111_FW_1_project_Ermolaeva035 191111_FW_1_project_Ermolaeva036 191111_FW_1_project_Ermolaeva037 191111_FW_1_project_Ermolaeva038 191111_FW_1_project_Ermolaeva039 191111_FW_1_project_Ermolaeva040 191111_FW_1_project_Ermolaeva041 191111_FW_1_project_Ermolaeva042 191111_FW_1_project_Ermolaeva043 191111_FW_1_project_Ermolaeva044 191111_FW_1_project_Ermolaeva045 191111_FW_1_project_Ermolaeva001_TAGII 191111_FW_1_project_Ermolaeva002_TAGII 191111_FW_1_project_Ermolaeva003_TAGII 191111_FW_1_project_Ermolaeva004_TAGII 191111_FW_1_project_Ermolaeva005_TAGII 191111_FW_1_project_Ermolaeva006_TAGII 191111_FW_1_project_Ermolaeva007_TAGII 191111_FW_1_project_Ermolaeva008_TAGII 191111_FW_1_project_Ermolaeva009_TAGII 191111_FW_1_project_Ermolaeva010_TAGII 191111_FW_1_project_Ermolaeva011_TAGII 191111_FW_1_project_Ermolaeva012_TAGII 191111_FW_1_project_Ermolaeva013_TAGII 191111_FW_1_project_Ermolaeva014_TAGII 191111_FW_1_project_Ermolaeva015_TAGII 191111_FW_1_project_Ermolaeva016_TAGII 191111_FW_1_project_Ermolaeva017_TAGII 191111_FW_1_project_Ermolaeva018_TAGII 191111_FW_1_project_Ermolaeva019_TAGII 191111_FW_1_project_Ermolaeva020_TAGII 191111_FW_1_project_Ermolaeva021_TAGII 191111_FW_1_project_Ermolaeva022_TAGII 191111_FW_1_project_Ermolaeva023_TAGII 191111_FW_1_project_Ermolaeva024_TAGII 191111_FW_1_project_Ermolaeva025_TAGII 191111_FW_1_project_Ermolaeva026_TAGII 191111_FW_1_project_Ermolaeva027_TAGII 191111_FW_1_project_Ermolaeva028_TAGII 191111_FW_1_project_Ermolaeva029_TAGII 191111_FW_1_project_Ermolaeva030_TAGII 191111_FW_1_project_Ermolaeva031_TAGII 191111_FW_1_project_Ermolaeva032_TAGII 191111_FW_1_project_Ermolaeva033_TAGII 191111_FW_1_project_Ermolaeva034_TAGII 191111_FW_1_project_Ermolaeva035_TAGII 191111_FW_1_project_Ermolaeva036_TAGII 191111_FW_1_project_Ermolaeva037_TAGII 191111_FW_1_project_Ermolaeva038_TAGII 191111_FW_1_project_Ermolaeva039_TAGII 191111_FW_1_project_Ermolaeva040_TAGII 191111_FW_1_project_Ermolaeva041_TAGII 191111_FW_1_project_Ermolaeva042_TAGII 191111_FW_1_project_Ermolaeva043_TAGII 191111_FW_1_project_Ermolaeva044_TAGII 191111_FW_1_project_Ermolaeva045_TAGII Factors Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 Treatment:ctrl RNAi | Days of adulthood:2 Treatment:ctrl RNAi | Days of adulthood:6 Treatment:ctrl RNAi | Days of adulthood:12 Treatment:tif-IA RNAi | Days of adulthood:2 Treatment:tif-IA RNAi | Days of adulthood:6 Treatment:tif-IA RNAi | Days of adulthood:12 Treatment:ncl-1 RNAi | Days of adulthood:2 Treatment:ncl-1 RNAi | Days of adulthood:6 Treatment:ncl-1 RNAi | Days of adulthood:12 TG(16:0_16:1_16:1) 1.019509995765360 0.669303198002034 0.455967764750425 1.119381074719680 1.135989395198720 0.858295907900796 0.954182948225321 0.611505414420816 0.221137911429919 0.752849522394612 0.851760118639075 0.461842040351629 0.852981931211548 1.130571879959310 0.992930440774429 1.116668030905160 0.693821945636422 0.376240249646141 1.324361610543290 1.168881993061600 0.781937923472160 1.320568628994720 1.005635373827950 1.056364267121860 1.703357138707140 1.887885556377570 0.318359672743534 1.396936982842290 1.062178497966610 0.671495524467045 1.404696067358210 1.097048288864670 0.910938277210420 1.892931377097780 0.930174503327840 0.526203138296072 1.322230769169280 0.742022290349602 0.507014577469652 1.354837284533010 0.782716364518391 0.648499581204429 1.625692957707400 0.731640330759755 0.136209465057987 TG(16:0_16:0_16:1) 0.690548103798406 0.224052456474029 0.343075424281670 0.505568016682267 0.172006294203888 0.372387964985389 0.732161693842520 0.147880394337060 0.066152366667070 0.482763125279040 0.490687894433380 0.214455347432843 0.445459717304165 0.196889641716214 0.251564591538488 0.753693122308661 0.274488836740182 0.170562246506251 0.788425648351191 0.202443443532862 0.335592101341087 0.516744246128367 0.161658981165680 0.368611403318936 1.172591570846160 2.753166436383960 0.172778113850762 0.645251844455723 0.241061786772563 0.207868113795565 0.840043910521693 0.217692645358837 0.394162158003663 1.274594861596170 0.236646066770500 0.314488594372262 0.945877930589612 0.193005797894169 0.151899104181191 0.885225810304781 0.163317943137531 0.335296376654795 1.747376562326220 0.223866502143372 0.091826605657070 TG(16:0_16:0_16:1 isomer) 0.350711438543285 0.319667199045748 0.315218872737432 0.250873801728984 0.286427872522127 0.277964805772569 0.233884985533027 0.186515812677373 0.007560270476237 0.067847790579757 0.132260887987434 0.219274568723469 0.177449612662374 0.273724623849371 0.254896572883368 0.206918817320936 0.334393566582502 0.214707769131398 0.265065800795370 0.334692929257848 0.303115446372595 0.294131227815757 0.332056285637613 0.236490458430479 0.297022093936801 1.612568912739180 0.161579532397472 0.231492414299579 0.287014189876083 0.296517750561320 0.305110288255520 0.301703609905768 0.250363585702652 0.446634893650228 0.239237082100104 0.254879645112160 0.346377833737041 0.257007720511924 0.184742153733881 0.328891659651129 0.312118735773949 0.305405614394871 0.299341667362291 0.246167379751524 0.053565519966624 TG(16:0_16:1_18:2) 2.272827617226250 1.769586280431820 0.834230412035344 2.949359009159020 4.012981629122020 2.573826789329210 1.350770532390020 0.936575830801381 0.345882374287823 1.997595526492460 1.958783751093900 0.876295071345439 2.426776426961980 3.569396536060850 3.233687895206650 1.561716883802150 0.993345594848022 0.461521372899266 2.429124911668510 1.832164029159220 1.016769120936640 2.795253962975080 2.829032170399400 2.452381922244640 2.186340891456540 0.196654745455997 0.347156025051995 2.358827876742260 1.719825185005500 0.869174024841259 1.943592160900430 2.809154953207980 2.215396754512440 2.152063886149560 1.306735397896960 0.526203138296072 1.468775237288800 1.708051309861350 0.880604266131500 1.904626327531910 2.533242762444370 2.110547735222430 1.280111520589960 1.216255555706130 0.325984450082598 TG(18:1_16:1_16:1) 1.763888227340180 1.322623036051780 0.791419290714725 1.547904091378580 2.068188724438490 1.607846042550870 1.439579034143140 1.027702127852330 0.599907462289371 1.549800108666070 1.518090472319770 0.833564642568558 1.300277334163940 1.915729353632500 1.933937505591130 1.673036895578720 0.918509387567392 0.602285391633543 1.621931830706270 1.400420323453960 0.971468350929310 1.483751022508940 1.516827287243750 1.534171519869160 1.853805286288170 0.000000000000000 0.621521270657603 1.686568583951590 1.361773099839880 0.785313506383538 1.174278362656150 1.723573854394600 1.451274251789710 1.886716410417270 1.356914728113620 0.736992715765846 1.308908544794780 1.313039443704890 0.756416485010391 1.115613599751940 1.611161753115290 1.208366554316390 1.354825253825920 1.186835551784610 0.585700699749344 TG(16:0_16:0_18:2) 1.398223975525670 0.400012124520192 0.409491307700302 1.463218264906610 0.545185167281020 0.710826030029090 1.338906801239790 0.410334787890221 0.353442644764059 1.325641446712180 1.396674977147300 0.444974765834439 1.314350924133720 0.599587271289099 0.657510985389778 1.448431721246550 0.486390642301822 0.363197254325075 1.500427215451160 0.413025316956493 0.574586972519479 1.539152452405740 0.632072052485632 0.783072997558067 1.583256900723990 1.258590370918380 0.353555214453875 1.518004854688620 0.390972085421751 0.414717266248994 1.608763338074560 0.565387659213655 0.758794252052654 1.811820794996210 0.357560115485353 0.349431771524735 1.621980817595570 0.521015651310165 0.405406392916017 1.667366175047270 0.662949872884201 0.742070663061581 1.830121413467020 0.404846701194143 0.388732630614929 TG(16:0_16:1_18:1) 4.619059954147610 3.380766047050790 2.754866334295980 4.333622067430110 3.906038585334380 3.504266234606090 4.265858757874130 3.966125531003860 3.037338663828040 4.000410113798750 3.856727493713570 2.864223853728540 3.790078968381870 4.066079813421610 3.652406884213320 4.530250531177370 4.290788037511840 2.978819469865050 5.645708078254660 4.950199981059840 3.827248877825400 4.659763903683870 4.300711453894170 4.510210885781290 6.153522946125470 1.022604676371190 3.399569369748800 5.324325528890320 4.461149691459750 3.690677981213410 6.086355880006230 4.195642477592010 4.071297078272360 6.923894235761660 4.631871737555440 3.692671632612860 6.151537104926110 3.587107756717010 3.312016278329080 5.823509535812780 3.966811374429370 3.481624004101530 6.760741072621510 3.755124698788050 2.782346151409220 TG(18:0_16:1_16:1) 0.380617065085736 0.139854399582515 0.096178409542212 0.239921950485993 0.090041555748470 0.109700614186579 0.355403488886057 0.202103205593982 0.153284483905696 0.253254925990978 0.312267956538332 0.135018516492363 0.231785907850025 0.094260058438355 0.093517609746316 0.341589444236517 0.230230864692498 0.185110202825901 0.297280002132909 0.124416246955121 0.145978400409659 0.259379421789580 0.104495782870609 0.120960043151304 0.392327444078262 0.000000000000000 0.191655722586309 0.282314312151556 0.119928238919350 0.110251617219020 0.243295736349207 0.099832007505405 0.114782074675985 0.460539564867640 0.198817242958281 0.167727250331873 0.306411160613536 0.133003995440023 0.119056054628501 0.257059103140261 0.082505805347997 0.126191000671243 0.556580807526472 0.200450580654812 0.259716249666746 TG(16:0_16:0_18:1) 1.563248660173560 0.603657255340854 0.822501337700928 1.083723884626220 0.249035199869108 0.648230902011603 1.788033766212640 0.406338020475706 0.485747378098199 1.240831708487480 1.390061932747930 0.490757368095383 0.990046459612829 0.328606753944483 0.471475360300610 1.634543059730750 0.486390642301822 0.375236942313751 1.471405412444370 0.530015246636288 0.667020528968264 1.376977357616910 0.449295456663174 0.770403865856434 2.036538444079720 10.501363407350200 0.443143866080196 1.700271870545180 0.480616937377798 0.701045403388963 2.090203598114120 0.426187009927864 0.632328543377212 1.955081043902880 0.402471047865156 0.602255935627926 2.501247626312670 0.541016252128214 0.565516259485381 2.099343280260690 0.572217682252239 0.718677892597293 3.638339778102650 0.493192485564899 0.512698548251973 TG(16:0_16:0_18:1 isomer) 1.027666075731490 1.311491767513580 1.611281586690410 1.264556777243050 1.385772448520890 1.286913394664610 1.308400063996360 2.014370776915630 2.630974125730320 0.973354841778822 1.066022757178720 1.683514637525240 1.278861001601240 1.485247645698970 1.152310215104550 1.418376474037920 1.892810642928230 2.595556068892180 1.124111169796420 1.863700444985950 2.075174979012380 1.433386086239110 1.757131178165700 1.522105680153320 1.414083447220860 2.989152130931160 2.407695012457380 1.286512440389040 1.730371638176800 2.248847682092210 1.642444343920950 1.599887638707620 1.302532603565850 1.592716884903640 1.849984945337260 2.756400033027470 1.408825227603540 1.640049267079980 2.032163691072690 1.754088851472690 1.768672836052380 1.523129276896980 2.309554815665160 1.740326179497710 2.539005646417980 TG(16:0_16:1_18:0) 0.187589839220827 0.122729371062207 0.109960071885151 0.248326859579451 0.132370061191688 0.142165206005818 0.167787054838911 0.074606325070949 0.000000000000000 0.155267059211367 0.154745238945298 0.080320354843762 0.154197594451442 0.138577378490158 0.122172649312290 0.150276236043138 0.109080254339747 0.054178595949044 0.203152621047546 0.151578256715560 0.097429964905477 0.215561927234835 0.124521107114105 0.149616412476426 0.237617675149441 0.000000000000000 0.030396149658930 0.160980357070397 0.120530893386282 0.042796376369675 0.198123563802286 0.149012148794922 0.128391582411617 0.243331746304723 0.121777720491388 0.087357942881184 0.293088936239035 0.114003424662877 0.095450112762505 0.215988552606712 0.105249341133076 0.074077106470245 0.153321341819710 0.088345784370756 0.000000000000000 TG(16:0_18:2_18:2) 1.498271889776780 0.946300534318015 0.649644104697471 1.059018545775750 1.505877713082390 0.857871534151525 1.173492492630930 1.272837195942590 0.652829355623027 1.296806135715780 1.274862699310880 0.739670147756200 0.955168432296432 1.613877638109390 1.078728960405100 1.350174182295270 1.142660369231120 0.764520187280959 1.211273318160150 1.406015494003860 0.771195645290274 1.109035896661470 1.355168306078070 0.941738789821373 1.421831849671380 1.014738486552950 0.716709213010570 1.361414870992870 1.358458500271760 0.666706406159010 1.055602347938580 1.442781002817670 0.874860242552755 1.442714977224890 1.545627011286450 0.828975490917210 1.313238267716490 1.383341555580330 0.652037168150748 1.097123670627270 1.528534971513120 0.818097167070517 1.661954671883810 1.427170394315540 0.791086207735657 TG(16:0_16:1_20:3) 1.719301656858710 1.320625116057750 0.837749134335669 1.453794578953340 2.132578906746990 1.168725305492600 1.296027887225410 1.330523872292090 0.689307660670868 1.782831174003460 1.582369263881660 0.896696441475754 1.130170464094500 2.087922036805920 1.390269216151440 1.416873711677490 1.149544942660760 0.715057135794146 1.463376046945820 1.589740548787960 0.859965168870820 1.566651707609060 1.766087959554610 1.125320541097890 1.624452573752620 0.173056176001277 0.658956528658602 1.479422785638690 1.538275526842420 0.841662068603610 1.426687782940260 1.892454289695510 1.120922710244620 1.646544816661960 1.436372531554810 0.794546184016978 1.335552993543780 1.659049837857130 0.769758973891171 1.341092558458040 1.856501596584110 1.045656839753670 1.264779386407990 1.448442000649470 0.688240409399739 TG(18:1_16:1_18:2) 1.881063909520150 1.358442887373430 0.859008081566798 1.571718000476710 2.179394532908570 1.373061265766640 1.517879659734630 1.502251645535760 0.742040547242618 1.828889385685490 1.615831268542480 0.933081562219978 1.291098905922790 2.075230544221420 1.641000812353710 1.793720272831820 1.423139678955530 0.860536698990654 1.881967185647120 1.745082829328000 0.987123764093609 1.565241489393510 1.837887849387660 1.516615723082610 1.828235558201430 0.346112352002555 0.813816912184100 1.745639062083210 1.560950401160710 0.928375678819309 1.331588472315160 1.969719848329920 1.477851309348910 1.921688765297430 1.670600317351010 0.976661801411635 1.584678589346960 1.659849861889850 0.913242046624486 1.346492272273210 1.973001729355550 1.198099727279280 1.495248133556030 1.640315320685760 0.721451031779046 TG(16:0_16:0_20:3) 1.773403653967320 0.829564923237916 0.705650434644308 0.900726091182286 0.625429842794399 0.850126713227328 1.944296053648470 1.176648326833260 1.047475474482580 1.619213617489970 1.562001087131590 0.860231000376686 0.875010158990325 0.717583850993590 0.708601366011281 2.044912781233160 1.179318487492840 0.952138658437834 1.752916901610250 0.917607970184130 0.842727559695235 0.999240335593255 0.824315165223198 0.930276242091325 1.885444596294480 2.517180741836760 1.163052673791710 1.572551917828180 1.010952868277440 0.851851682024962 1.212516210469990 0.838883208030673 0.871778844574877 2.015123942341710 1.073544018232590 1.123320371401570 2.313737318241560 1.145734417861910 0.865927528362642 1.268114608107590 0.988375996617504 0.890224876002072 2.730580087646270 1.202531938716500 1.108041041595310 TG(16:0_18:1_18:2) 9.890062566920640 6.461558677854180 4.541790809144270 10.084617441075300 10.730949197963400 8.422757988658120 6.277608597205310 4.401773179186010 3.258476575257960 8.634936116477540 8.462051613436020 4.697134351263180 9.424410118020270 10.291085419459700 9.385636118304600 6.213344374860510 4.501795742628070 3.536658346673730 7.835886811833920 6.398840501616600 4.971426414407660 9.678126153608860 9.006298554311400 8.487111656122410 7.570189194163170 2.949821181839960 3.186796322136280 8.549254334994800 6.354991353791690 4.550681353975450 8.035891747820720 10.048201934788500 8.272269654780460 7.290471931493450 5.165620895453860 3.639229126379670 6.627806626314540 6.340190459321400 4.905930526933070 7.569417002717040 9.612773156820910 8.286239137794460 5.234828670701530 5.009977927277520 3.246070509977420 TG(18:0_16:1_18:2) 5.568971400868720 3.479805795326570 2.490082481196530 6.249559299416190 6.242706699421550 4.867036436952910 3.128635386188180 2.699150260602560 1.576316394295320 5.413470829142540 5.024591134642620 2.465031690155040 5.880680163631060 6.376823298413690 5.889443626143760 3.696795406661190 2.394400992801980 1.663483557102140 5.155239607439870 3.742660446017090 2.722209874153880 6.084084301394420 5.934777912163480 4.772039607615030 4.083408091426810 0.000000000000000 1.507009104142760 5.179310165909550 3.429857234923370 2.540270625942860 4.630147686059420 5.995439418798170 4.575234039237960 4.111358466785000 3.051352386496990 1.554971383285070 3.277267196127390 3.554106765367230 2.600758986453640 4.719022619073920 5.618137243931080 4.696748469885380 2.565090385364670 2.713845259699720 1.505956332775950 TG(16:0_18:0_18:2) 3.199902040042220 1.351450167394300 1.696317375614930 2.606795290046850 1.396242396863740 2.044420537113520 3.309980990913060 1.589381175172190 1.517724298104490 3.243646295793770 3.578979628939960 1.787931098822130 3.222240207862270 1.621080917684370 1.839809032598260 3.155800956905900 1.645144819550280 1.605291731823540 3.760258276246720 1.431346357038880 2.312504380705210 2.997721006779770 1.941364161205870 2.652071569541810 3.148434195730100 4.877037687308730 1.660589649787880 3.711861125838080 1.371038912268950 1.956405776899430 3.510749550576510 1.755031974695750 2.472821877247740 3.189647306539830 1.293780321248940 1.642329326166250 3.723561712673190 1.808054313951590 2.009584094505220 3.609626871593990 1.954976267483340 2.581002341226440 3.640773450195020 1.266003667293550 1.753888168050030 TG(16:0_18:1_18:1) 5.562446536895820 2.477420792604550 3.087678818535030 5.486113390093720 2.526248964437980 3.827851218425310 5.086151025975470 3.012230374739580 3.460713810497280 6.004529466308500 6.205680864370400 3.527669984738010 6.648853417894320 3.363588548204180 3.800124723836360 4.961427731516520 3.175844782088360 3.353053104846410 6.066524221853150 2.971544213866790 4.356035439491870 6.143514926192800 3.924235358116440 4.976555590798530 5.298615875750850 7.472880327327890 3.028416384439750 6.083993164321700 3.203861809824100 3.834351530454460 6.468734358144640 3.743700281452670 4.824313709116490 5.760958097578050 2.762886013134410 3.421348139428950 5.598664793384290 3.031091053975260 3.788240496843080 6.396206455603310 3.894225621923800 5.028146051462800 5.436823454368770 2.885390472070120 3.265966274536450 TG(18:0_16:1_18:1) 1.223140125586230 0.627775003840288 0.546721477412970 1.353190364046790 0.577791577834452 0.507763191002869 1.039601812506950 0.775905780737873 0.737882398480688 1.357086288115490 1.269836785567350 0.623366273942433 1.577465867046890 0.657899534515155 0.571434800647029 1.106726679905390 0.873893775341545 0.696696611611414 1.433580329192180 0.669284128142061 0.776025506798409 1.606943656624920 0.732053034981343 0.769378269480588 1.348351166432240 0.751221127641909 0.609682770264125 1.432059969839470 0.650414833435722 0.646429075450521 1.543382562019810 0.741994650378008 0.562355130962881 1.406057207651710 0.694219373978568 0.636993564679502 1.383846056901350 0.723521734592907 0.708691428629138 1.394107930461500 0.877077842775631 0.766763031884996 1.296903858027360 0.665509651390966 0.653346299250052 TG(16:0_18:0_18:1) 0.796577143358004 0.329656799015927 0.476493644835653 0.612539586962646 0.193694044342639 0.485907942915408 1.155866377779160 0.494266903595039 0.629392517146693 0.991621554627218 1.243252347081880 0.508427846161011 0.822387170407690 0.257259984820838 0.328755492694889 1.071585160092220 0.469402733839074 0.384266708305259 0.935469450252272 0.383523508602458 0.586245258918425 1.005284127945630 0.351717513076682 0.457295325230366 1.234578790450360 1.455245116374380 0.667115495145999 1.246599955164970 0.312627004720668 0.528840936568128 1.313559228009160 0.322553046362671 0.403149568772476 1.193484279494590 0.380879253451789 0.517981214260195 1.681930827280820 0.468014059142337 0.711257291875442 1.472649222318490 0.458500003326847 0.569224081297675 1.727907185587210 0.373968562967471 0.688699542428024 TG(16:0_18:1_20:4) 1.586901292075310 2.037450268203630 1.273630859288410 1.028964628411260 1.478506276700380 1.100188944985320 1.727020291725760 2.396728192904250 1.633018422867100 1.402622593716130 1.527613256254860 1.361430014601760 0.905604919794182 1.551792228439290 1.172857433397980 1.958214952746740 2.203063497484720 1.659470327772580 1.525579444723710 2.336746682386860 1.479769637923350 1.246028523315380 1.636251039095870 1.271136214063830 1.664615126454510 1.061935625462380 1.742179314661850 1.504700692947260 2.177842579873370 1.407185613488600 1.240253509402310 1.723144601291080 1.194041716428030 1.746510975641690 2.718838752531140 1.960928882556450 1.828475295400340 2.696080990272950 1.330143506883940 1.418652084166810 1.786819274178770 1.178735711728290 1.912866264607810 2.736146137307880 1.744705507484330 TG(16:0_18:1_20:4 isomer) 0.300198116619764 0.224651832472239 0.393539766605496 0.186729063693000 0.184443097128282 0.248927031978694 0.502208687776425 0.412093365552608 0.552504566403368 0.245569858942617 0.210572559764794 0.374461526317101 0.172358644464612 0.167280732529901 0.260361022288973 0.483380852798450 0.393547252050680 0.589101933285942 0.318117656691786 0.311132174724073 0.608595859414690 0.267941460955449 0.235206535659976 0.421097806082843 0.492023555608354 0.129792132000958 0.721508605061980 0.353491577467806 0.314660963546561 0.653867493248107 0.408867598618778 0.249193690970340 0.430349325506377 0.545768879125282 0.443158625257705 0.623951537677594 0.494321135415881 0.252427582924591 0.447917615180780 0.343961770026189 0.266401809320857 0.334269693951084 0.763029211122758 0.394150857202849 0.557892542669528 TG(16:0_18:2_20:3) 1.234830506871010 1.605756840920870 0.881439936231369 0.976752314345841 1.256393801141440 0.737349389358533 1.361108926678070 2.496780603847610 1.404509247722850 1.122359028013600 1.140353376227660 1.069224563680150 0.537611136845384 1.211042803211270 0.785958866234927 1.569693084022900 2.430075600573750 1.613217859749410 1.082900209526780 2.115381389357960 1.023764092776010 0.740263833293834 1.325967742363000 0.796888384032705 1.353387628025080 1.887885556377570 1.884561278853680 1.108369714672300 2.076219970387070 1.065935460007550 0.874319287059488 1.427757144194310 0.865551852827913 1.417433756829590 2.811769835686270 1.989294520480230 1.672605270218670 2.318269640820010 1.096342047880730 0.919423997800844 1.694756344750870 0.756236285176066 1.761978594880480 3.043212067450890 2.108951043257370 TG(16:0_18:2_20:3 isomer) 0.206240075410028 0.191457818857042 0.165907756460315 0.198865243035524 0.230458520095088 0.162492708595904 0.195615978479870 0.252355894552486 0.150298177067583 0.191944009080540 0.187955947918942 0.186431575627855 0.162250135495017 0.221984495702204 0.213013567378219 0.196029570063041 0.222255488719461 0.164121013432309 0.244798908362293 0.251324888064290 0.219883608831548 0.229976371995258 0.191740659147456 0.191430580011672 0.310814250298736 0.000000000000000 0.156268205193912 0.241231060694251 0.254109255981628 0.225516524241345 0.218351979666499 0.227632920799025 0.188228479394550 0.279315350000694 0.223829177606725 0.162547438189271 0.174154778135672 0.206796212158213 0.187605657116756 0.191362585055719 0.237052970057772 0.176680396923331 0.215988398198401 0.212758949642388 0.177607959775049 TG(18:0_16:1_20:4) 0.197186826647631 0.261913040361009 0.217764926361353 0.195974463695804 0.256431470519875 0.177059337539634 0.178667791122404 0.236368824894425 0.174113029067728 0.202512607228540 0.189410817686804 0.200969559854576 0.176421628699364 0.249823456192950 0.211114338011637 0.195174151488642 0.233217160180160 0.177424868659796 0.228053328027374 0.239819182806216 0.212130848376249 0.242235197483333 0.242109811071916 0.196359475535593 0.225517253322538 0.000000000000000 0.151388823274978 0.227926898952896 0.255615892148957 0.199023529345832 0.189049504580141 0.211204791308424 0.199276575061070 0.264515302227615 0.280788331269187 0.162496051164047 0.203696810686129 0.281168446300127 0.216723073235813 0.190331730600096 0.229032244405907 0.160266469647555 0.189169331740414 0.271496030358013 0.181403459475542 TG(16:0_18:0_20:4) 0.479305632675823 0.442824695820962 0.258479475644694 0.226295809985992 0.152337748388400 0.163065613157420 0.577424743268858 0.669724993092254 0.530541980669900 0.485372655685954 0.462913107956019 0.363048003893802 0.217345180750604 0.156825686746783 0.129725140360686 0.550820203650425 0.772055734609601 0.558340530474873 0.438616182775988 0.434795616914298 0.307612213983617 0.301182318893532 0.172581885298496 0.157459208291722 0.625296077757388 0.031464759272960 0.523453693073791 0.450212833327457 0.417639545583466 0.336664827441444 0.364151110268602 0.201626314912635 0.138534517422134 0.617493808155102 0.647062894979775 0.524353205387999 0.750041232284439 0.521215657318346 0.364557850034859 0.358017388714762 0.229612930425951 0.179084653887716 0.987340767877086 0.878911895579743 0.717318834524477 TG(16:0_18:1_20:3) 7.229005543306940 8.418378603441370 6.757412950915470 5.947109919159160 8.181516776480600 6.514137051311360 6.643689444126570 9.085984588997750 7.900482647667200 7.177513384216220 7.169862737798790 7.655333020158920 6.375948151523910 8.273481111838140 7.030480637698140 7.634032790991410 8.417061593068650 8.040504961771130 6.076198156188750 9.726441023204500 6.304634737601410 5.600580913204130 8.228587780054890 6.756870240870840 6.639089499691720 3.618447316390350 8.576513595869780 6.255616850785180 9.546046756193500 7.542351854484170 6.179473954993300 8.664167285281710 7.657915932940870 6.382454765629080 9.179967312787010 8.426444396268540 6.531220499599400 10.041301640701300 8.191261827500590 6.021499042368940 9.242585819042510 7.017831139286400 6.381088226210790 9.882719684658730 8.573544081515090 TG(18:0_18:2_18:2) 3.575897326480490 3.960590971034210 3.335895354137110 2.969734546355280 3.703370299555020 2.898472707521600 3.542171157710340 4.582959968644030 3.704532533355910 3.488942154043660 3.377943079199060 3.384699753116110 2.840417593030130 3.828714600760250 3.221470630275430 3.632061028058000 4.597464490286700 3.864739844365160 3.341376919515350 4.510724766958350 3.076122139836170 2.808348846405240 4.120119438898280 3.105747142857420 3.374429267203750 4.011756807302340 4.340250211825170 3.199650898795910 4.681871889973370 3.388046462599280 2.948078629378020 4.082810233237000 3.458869230168950 3.190700690722970 5.025706067655240 4.212708327882030 3.880097849073590 4.915147651035440 3.504969194451130 2.744036384253450 4.399906364379270 3.050157348871330 3.906043708264040 5.545198989873170 4.384720420125090 TG(18:0_16:1_20:3) 0.269585629813582 0.452614503791738 0.312521185640517 0.327549495140670 0.495983888604177 0.382159170562356 0.204395139531037 0.411134141373124 0.307324994859016 0.287309297801202 0.262141079991094 0.342325352344112 0.279880871833689 0.523232507794274 0.446318901146761 0.236396079006321 0.351828525267954 0.287447550729652 0.259938615597503 0.405598999681167 0.356993384230580 0.293758527287360 0.511650675389377 0.390709988758498 0.280363028668172 0.058996423636799 0.265726339913071 0.288208055802976 0.440412351252636 0.315501000365298 0.237332217078758 0.448134107892351 0.366423156623633 0.269834892352458 0.419018999103560 0.333152361933700 0.220782563446427 0.460803842547430 0.372347810850637 0.216643063372187 0.449305808009475 0.356609789744479 0.217059213919047 0.450983786061162 0.325663056962798 TG(16:0_18:0_20:3) 1.810106013814880 1.646571492227610 1.260582264091370 1.168027669775760 0.760716532453022 0.890336125970764 2.311732755558330 2.803066213379950 2.426846822871930 2.182741708862990 2.067105418355610 1.672992671040710 1.197846074992630 0.849849784522810 0.743476104087699 2.269171164251380 3.033682811268530 2.380848299760780 1.850236681026360 1.630330967868170 1.659307248467120 1.244215385609670 1.096077020047670 0.968404295593382 2.303987468663680 0.770886602187509 2.822202505964160 1.847282857787160 1.799300242832080 1.618620091981640 1.718127545293420 1.141935899118950 0.964349175493652 2.157014791810310 2.355837504853620 2.721148533723650 2.301747316304510 2.127163900003560 1.834181682987880 1.597988033906930 1.314527977875860 1.230199806749720 2.981491680370270 2.817715885790000 3.252345328030650 TG(18:0_18:1_18:2) 18.155434004589600 19.976345768939200 18.616973237301900 20.273659510281300 20.431356337609700 22.619120836149500 16.677016359746300 15.773908729286400 15.498554476284900 19.036524318435700 18.900080893404300 18.867251352799600 23.937340852938100 20.553357720619500 20.913736241367400 16.218273782194000 15.047710496212600 15.079709205817300 18.312757697285900 17.426413003607700 18.928060703431500 19.531522285436700 21.547248886002100 20.143919405596200 15.135212786692700 27.924973854751600 14.590151836286600 16.457248074056300 18.810352549096600 19.329696660303300 18.088681375148700 20.561223658822000 21.910023538542400 14.115348054040200 15.891560688237900 15.724429718613100 15.453780274421800 19.570587900460500 19.880308432362700 19.537146349425300 22.066068761693100 25.524111773256500 11.876319810796600 16.416876823847300 17.202184126424400 TG(18:0_18:0_18:2) 3.335836706144270 2.921244448422530 3.059822266990800 3.057604050514170 1.593675708471680 2.313897867900660 3.728601218642460 3.517155324773320 4.044744704786560 3.941695679643190 3.995601426100380 3.687507490877090 3.695847105105990 1.865992423233810 2.015848713652780 3.256370437950150 3.850890618371220 3.749359501140340 3.692540735897540 2.804706401106040 3.794772222856900 3.715924997987420 2.628050734355580 2.617683926351660 3.593967336635300 6.056966160044710 3.732327218646560 4.031161007630600 2.968073249637180 3.495037403523470 4.150688661657890 2.560188153163790 2.558202279551460 3.735300313404970 3.097126990653330 3.654645233946930 4.516234062956040 3.116093607451970 4.039695094980870 4.182323791384510 2.390490782516750 3.139829635651100 5.568241747357090 3.171870976728690 4.511747224617370 TG(18:0_18:1_18:1) 5.353107151098670 5.920978610896460 5.877732375834260 5.786652563738600 3.689908937399930 4.672354979474790 6.411499277329290 7.637822529138430 10.023028583870600 6.616464346729770 6.415975676270420 7.382243813690130 6.848331258335470 4.792582010555660 4.701425677626570 5.830717958473750 7.835896303553610 8.742820094443930 6.236785466159660 7.229977654211320 8.266557791595460 7.007777232582940 5.721417184769140 5.958714943667970 6.104449730605480 5.584994770950320 8.986061717590110 6.991336995082120 7.333551544471570 8.808920802758130 7.283022205372030 5.896711204987540 6.044675699938910 5.468117294665880 7.566628434220240 8.572383547905340 6.461278821633270 7.270218397360660 8.959994456093240 7.160347774295230 5.366506635245100 6.544777336564130 6.746139040067250 7.429623147762010 9.874420994990250 TG(18:0_18:0_18:1) 0.933327417456665 0.628345838124298 0.883052683952351 0.534857851401895 0.172006294203888 0.433922158629698 1.011806785240700 0.835324389633664 1.266345304769620 0.887240338350669 1.031634926301980 0.682723016171973 0.797911361764604 0.273038597223182 0.310429595298046 0.989511215791739 0.845819021355741 0.768533416610517 0.748762517575241 0.599191900707819 0.719482817763521 1.047590674412280 0.487889717932458 0.345083015873047 1.199710979422990 4.877037687308730 1.262240109520850 1.088280430442850 0.458017394867870 0.813131151023827 1.157041612605350 0.315194421730823 0.318411124380809 1.080772171898900 0.696119451886944 0.910578086973280 2.111572563358500 0.677020337690944 1.033529715611210 1.155211501063170 0.514149080247784 0.787556605631030 1.701136792571070 0.676745862801227 1.513608549914040 TG(16:0_20:4_20:4) 0.032080581200083 0.054086548409973 0.048675658487827 0.028653099182244 0.041356295954239 0.048484700854223 0.049373459409769 0.082908943246702 0.069614970545186 0.028353852636321 0.016573611873705 0.076007954992200 0.023496776297363 0.043494088100376 0.051756776890479 0.051510069831402 0.078376845044444 0.082030407496183 0.021369720947335 0.069675132902340 0.075770534245721 0.021487696410157 0.044807209140030 0.061313771100017 0.031422354737697 0.014473789265561 0.094279257457899 0.021503516622552 0.062723523600137 0.074754060943059 0.029124163878936 0.045194219613933 0.065479707029925 0.047749905021615 0.079829182305100 0.108457455438251 0.036742694824875 0.057133716296838 0.061203022841438 0.033759665283195 0.053510216747106 0.051765601840751 0.051912659402480 0.107756982876528 0.080548768038954 TG(18:1_18:2_20:5) 0.109155536879945 0.235397787868733 0.323942371773654 0.103163891766827 0.163263887337612 0.204028289305812 0.274204723256438 0.343442223929286 0.375726541992766 0.106641069608937 0.100428337466618 0.303315362403594 0.066757768074017 0.159192478607134 0.219383205049182 0.300610270638600 0.360367184521598 0.466588074927834 0.124387447687112 0.305781157070893 0.321085862121827 0.088692652771155 0.141233150437314 0.272657812978712 0.195685903888015 0.043657353491231 0.440792163975005 0.129356364611196 0.317546171806995 0.356157557916489 0.119000937362205 0.180448193222177 0.241545651822622 0.176083700053236 0.419882670880095 0.502729545173645 0.159600248006529 0.246427402679176 0.299097546895154 0.127241801639059 0.151538485268419 0.201779540033153 0.172036280210084 0.391800687793374 0.415959219192250 TG(18:0_18:2_20:5) 2.585477349260960 6.721573694220860 11.465170161891700 4.092936034299260 5.185615843520700 7.621752536908930 6.236085538179520 6.580677547999180 8.126345728144770 2.636408570104940 3.056020077837650 9.301579013036650 2.192910075377290 4.199786402865830 7.264774459265660 5.471788948539950 5.957838317974610 8.993145273875160 2.757651721705400 3.998614066081750 7.603367842443990 2.508778205472340 3.197206859036190 6.202203589133640 3.258978070690920 0.000000000000000 7.892760208278900 2.638481356545550 4.303856875590650 8.613484017336610 2.418296219770700 3.274955892403960 5.848621753596370 3.252955695946390 4.463542108308830 7.130566394164010 2.627475702261070 2.826984922627080 7.376241025944610 2.149413353819520 2.411782603251720 4.202381254073430 2.192008453603380 4.162287260349190 5.942252696411750 TG(18:0_18:0_20:5) 0.126527987207787 0.196566785698934 0.294311797736336 0.162202010792998 0.063619893251847 0.114973458021272 0.314524460979849 0.359149519868330 0.562446322079619 0.240416036388962 0.260897827644012 0.323899862942953 0.116394708002196 0.059759779407315 0.097243875550341 0.278346268283286 0.374440325532316 0.679028369488031 0.245611518846483 0.168760516822445 0.358817073317273 0.151145173745734 0.071399383348175 0.119481977786113 0.318975900879956 0.049556995854911 0.665515697795529 0.205589211389161 0.140516422145894 0.329664563020976 0.205315449168309 0.061708199725239 0.117882731391226 0.299382318689460 0.282602041999909 0.559306659945518 0.395803286166442 0.159844801737844 0.334424352070266 0.197645888404278 0.059629195683325 0.100705876848760 0.445848727323348 0.334684709334650 0.643092328285013 TG(18:0_18:1_20:4) 1.217974608274350 3.239484561758250 4.046530645373550 2.040635519627200 2.275284302659970 2.920136987421890 2.045476732172540 3.419767425439640 4.208991580882800 1.534534355785620 1.502615948425240 3.442610728958460 1.452516863923940 2.042232663501740 2.352878626687460 1.756382408030340 3.289753178833310 3.781866658709770 1.423519437483160 2.636342632740770 3.007421523556660 1.457460525776090 1.818226621948590 2.182046783411230 1.291658688502560 0.000000000000000 3.759843733074640 1.438951525621490 2.667936258743670 3.180769346382150 1.380128745446720 1.697757346311200 2.091884052232470 1.234039570545380 2.773681910341090 3.125872744389650 1.132921960807610 2.351230630968150 2.957065020288380 1.206754223844320 1.520066633720800 1.827885092112280 0.914573972315033 3.008731479764440 3.363608565218470 TG(18:0_18:2_20:3) 0.276162148959600 1.095401022215790 1.128306162150700 0.388077575324318 0.815713675391083 0.593847406042511 0.574204587670939 1.409260190358040 1.424732971246780 0.359775957201196 0.309146599581828 0.876086238422845 0.313804342613006 0.645153159800566 0.467821287425724 0.513019950430343 1.383888669402130 1.442555282509920 0.241558140359868 1.060742605888020 0.752542387051961 0.252026141094186 0.519347681834969 0.481547663059206 0.341704548068164 0.031464759272960 1.629553581188760 0.271271857906231 1.092235889504810 0.776346646572749 0.306992462111642 0.501582251468340 0.498127241861469 0.314245569513528 1.347604346362580 1.295281912611920 0.272039821727322 1.001610088367050 0.776512325955443 0.259660783433024 0.461754264564180 0.434962574816253 0.346992966931020 1.416603209233520 1.451748026569720 TG(18:0_18:1_20:3) 1.409857264250680 5.593575180216670 6.259776183457740 2.465694694962740 4.574021289607920 4.855047878536000 2.433420740784930 4.284268217199260 6.890808525565810 2.094017675027920 1.963941925725410 5.778969210653800 2.273435485813040 3.997957369441060 4.591303694178270 2.220157991880390 5.329375114897500 5.920115245498720 1.692841769386190 4.587938120547010 4.813123539804830 1.682491061029650 3.874208457188140 4.265455327145460 1.528501523406950 0.000000000000000 6.420946645846480 1.450020588190290 4.580098616870330 4.554655303209780 1.534467001648700 3.303715850340100 4.427904698420630 1.446928513957440 5.141956288776810 5.419892324449540 1.310240767232230 4.592537959840310 5.298404969087710 1.519937625124050 2.897260311259800 3.710223355471990 1.107077434822000 4.724269376074620 5.996124305063900 TG(18:1_20:5_20:5) 0.489364797967374 1.555523423927970 2.888284554849960 0.435590781123853 0.737420897390191 1.344256897534860 1.080226617602790 1.271464972463600 1.790593360268060 0.218861315227855 0.270790942065472 2.488918963685580 0.145826867895506 0.452887337544864 1.083538120146220 0.853534341593629 1.113485871697460 2.423358431434130 0.305086867141736 0.796925227941399 2.003035168245190 0.264869199842990 0.350224716178531 0.805364636433083 0.328971340041134 0.000000000000000 1.725765393846030 0.204258795215025 0.670099034961869 1.573816361767960 0.252250921433071 0.330721119704022 0.775690584298023 0.348617495409299 0.795398522599604 1.916684653838400 0.304272943601429 0.444171342907141 1.292330893395810 0.222877278413335 0.218011307650478 0.477225513455067 0.202797895457721 0.785359714013546 1.095231230106150 TG(18:0_20:5_20:5) 0.209339385797155 0.448104912948057 0.887011246540216 0.193631276918234 0.195227143921413 0.363953536718626 0.403180429046753 0.479465541603285 0.547911702089054 0.094437600661005 0.127471721233409 0.778769293290595 0.041780205353748 0.122620399165004 0.358043608716390 0.382139752576188 0.541448453629586 0.928640197405935 0.162270574545311 0.303909318196017 0.673465895877396 0.109110597934941 0.136405226810609 0.345276069308500 0.222534118379085 0.000000000000000 0.838805746798442 0.113657453756397 0.244549649499929 0.591639524083916 0.115902284824337 0.130554265343370 0.295878401667570 0.252253910335896 0.351920338784582 0.718873650671784 0.150108163139697 0.181475451522564 0.424362990579710 0.111659536590015 0.090659604879456 0.227091817273872 0.216110081803020 0.309639108328571 0.584629389350012 TG(18:1_20:4_20:5) 0.037517967844165 0.169823199493053 0.282963918317788 0.059725793406545 0.139100742269231 0.197333793411061 0.086944201143799 0.112708841089327 0.187872721334478 0.021424244640762 0.031769065294582 0.282181470637103 0.028844740485877 0.077157414647466 0.183519979173784 0.092500803139784 0.156753690088889 0.231081744795998 0.028130833654484 0.115544341070447 0.252800780478972 0.041156211322247 0.080944545173039 0.207024471260425 0.041270574252314 0.049871643447641 0.237022775850937 0.026951570855637 0.115376691057851 0.200896380292676 0.029239075545941 0.087787165420508 0.193972718791646 0.031030591266859 0.115868478196338 0.227293034490786 0.039433784148525 0.068242049991182 0.131105348433144 0.035765740779375 0.063703675923971 0.113790233128452 0.020489085345717 0.103423750812052 0.125617266095444 TG(18:0_20:4_20:5) 0.251751001620994 1.031782968348550 1.832667864752510 0.288541802649500 0.410256699426412 0.682795082955360 0.733670082517335 0.940426050077364 1.455144159237440 0.185341897151048 0.194489635785522 1.459461007688570 0.125535198739956 0.266575540377857 0.588416243571213 0.705324981781607 1.063226697460200 1.785042266879810 0.211501226379167 0.642559558988250 1.271277840373060 0.183487521220762 0.230224234988194 0.574429290607777 0.324345543778170 0.000000000000000 1.496063290670840 0.224348079444471 0.578269560563196 1.168371643732390 0.152733455335182 0.224370597212239 0.527618788341418 0.350218639367668 0.778574196392709 1.549287978295270 0.202091482649002 0.402590093806418 0.953041664610555 0.194798766574462 0.203300595142682 0.345758143445768 0.208005953735407 0.744909352936605 1.147021435696740 TG(18:1_20:3_20:5) 0.035228828067007 0.152923650514930 0.313984387663735 0.057306198364489 0.066708528012986 0.128373059154507 0.097041931171377 0.133436076901002 0.175129885446782 0.025834351028446 0.027907047365349 0.253009117757849 0.016984987407870 0.051163179754542 0.103612957891081 0.078305478688940 0.129412098468319 0.266618890509241 0.036277253758490 0.099797496535547 0.211514624666591 0.029715312399195 0.042016771230900 0.090493797868806 0.038096312048416 0.000000000000000 0.191975682056403 0.041481045893371 0.090081023126489 0.184003020201418 0.035067870793005 0.041576229169941 0.104639139665468 0.051952907912333 0.098734957493444 0.205876977858338 0.025238954077493 0.060259810204699 0.158219338529487 0.028927739557076 0.028524990972147 0.053126281322757 0.023582282575127 0.086630332247052 0.137280775457319 TG(18:0_20:3_20:5) 0.137022143430865 1.238710396302270 2.316492181047180 0.373725556311700 0.574702943073313 0.963827050000787 0.667843322820938 1.116496977244800 2.168002062441790 0.181779888145611 0.227911962179946 1.886178956867020 0.235542944476738 0.435263313518072 0.909336582133615 0.591244511146029 1.186891518265440 2.244870057002000 0.198721959121842 0.873273873354169 1.542574492223960 0.246103224588855 0.393457570736217 0.807397730425202 0.268340090865774 0.000000000000000 2.184987223298450 0.208941860147982 0.751095795317450 1.647711438299600 0.255678459086850 0.409047546722851 0.717099085664482 0.268779401400955 1.173866404451310 1.941564195970960 0.193538614600572 0.565226979418462 1.422730116263570 0.227278863311154 0.270865833099950 0.570393719820889 0.256314344769077 0.882016863923647 1.635003322592680 TG(18:0_20:4_20:4) 0.028546279881430 0.131577302464366 0.181800652183449 0.046810249766264 0.075651359604682 0.149759374249024 0.066335205317121 0.116092770833616 0.186247263182088 0.030714172889375 0.023330820640983 0.174889540636806 0.031303335464075 0.066735984169030 0.121938855287924 0.038424477585184 0.134776701140766 0.170301386599829 0.026922559255968 0.090367090899619 0.173733449386578 0.035616068332567 0.068055518296316 0.131009681050628 0.032645310924471 0.016558329567395 0.207300140876555 0.026874406717537 0.085049611645695 0.163950879949541 0.042596566217492 0.063774746809349 0.112984592522223 0.036805243358818 0.112052776287608 0.168282230204294 0.034937533422130 0.065691973386881 0.119969501944185 0.026929845445464 0.059106578265285 0.092476820038767 0.023460598970508 0.093269131965786 0.157237757753456 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Standardized name Formula Exact mass Super class Main class Sub class TG(16:0_16:1_16:1) TG 16:0_16:1_16:1 C51H94O6 802.705 Glycerolipids Triradylglycerols TAG TG(16:0_16:0_16:1) TG 16:0_16:0_16:1 C51H96O6 804.7207 Glycerolipids Triradylglycerols TAG TG(16:0_16:0_16:1 isomer) TG 16:0_16:0_16:1 C51H96O6 804.7207 Glycerolipids Triradylglycerols TAG TG(16:0_16:1_18:2) TG 16:0_16:1_18:2 C53H96O6 828.7207 Glycerolipids Triradylglycerols TAG TG(18:1_16:1_16:1) TG 16:1_16:1_18:1 C53H96O6 828.7207 Glycerolipids Triradylglycerols TAG TG(16:0_16:0_18:2) TG 16:0_16:0_18:2 C53H98O6 830.7363 Glycerolipids Triradylglycerols TAG TG(16:0_16:1_18:1) TG 16:0_16:1_18:1 C53H98O6 830.7363 Glycerolipids Triradylglycerols TAG TG(18:0_16:1_16:1) TG 16:1_16:1_18:0 C53H98O6 830.7363 Glycerolipids Triradylglycerols TAG TG(16:0_16:0_18:1) TG 16:0_16:0_18:1 C53H100O6 832.752 Glycerolipids Triradylglycerols TAG TG(16:0_16:0_18:1 isomer) TG 16:0_16:0_18:1 C53H100O6 832.752 Glycerolipids Triradylglycerols TAG TG(16:0_16:1_18:0) TG 16:0_16:1_18:0 C53H100O6 832.752 Glycerolipids Triradylglycerols TAG TG(16:0_18:2_18:2) TG 16:0_18:2_18:2 C55H98O6 854.7363 Glycerolipids Triradylglycerols TAG TG(16:0_16:1_20:3) TG 16:0_16:1_20:3 C55H98O6 854.7363 Glycerolipids Triradylglycerols TAG TG(18:1_16:1_18:2) TG 16:1_18:1_18:2 C55H98O6 854.7363 Glycerolipids Triradylglycerols TAG TG(16:0_16:0_20:3) TG 16:0_16:0_20:3 C55H100O6 856.752 Glycerolipids Triradylglycerols TAG TG(16:0_18:1_18:2) TG 16:0_18:1_18:2 C55H100O6 856.752 Glycerolipids Triradylglycerols TAG TG(18:0_16:1_18:2) TG 16:1_18:0_18:2 C55H100O6 856.752 Glycerolipids Triradylglycerols TAG TG(16:0_18:0_18:2) TG 16:0_18:0_18:2 C55H102O6 858.7676 Glycerolipids Triradylglycerols TAG TG(16:0_18:1_18:1) TG 16:0_18:1_18:1 C55H102O6 858.7676 Glycerolipids Triradylglycerols TAG TG(18:0_16:1_18:1) TG 16:1_18:0_18:1 C55H102O6 858.7676 Glycerolipids Triradylglycerols TAG TG(16:0_18:0_18:1) TG 16:0_18:0_18:1 C55H104O6 860.7833 Glycerolipids Triradylglycerols TAG TG(16:0_18:1_20:4) TG 16:0_18:1_20:4 C57H100O6 880.752 Glycerolipids Triradylglycerols TAG TG(16:0_18:1_20:4 isomer) TG 16:0_18:1_20:4 C57H100O6 880.752 Glycerolipids Triradylglycerols TAG TG(16:0_18:2_20:3) TG 16:0_18:2_20:3 C57H100O6 880.752 Glycerolipids Triradylglycerols TAG TG(16:0_18:2_20:3 isomer) TG 16:0_18:2_20:3 C57H100O6 880.752 Glycerolipids Triradylglycerols TAG TG(18:0_16:1_20:4) TG 16:1_18:0_20:4 C57H100O6 880.752 Glycerolipids Triradylglycerols TAG TG(16:0_18:0_20:4) TG 16:0_18:0_20:4 C57H102O6 882.7676 Glycerolipids Triradylglycerols TAG TG(16:0_18:1_20:3) TG 16:0_18:1_20:3 C57H102O6 882.7676 Glycerolipids Triradylglycerols TAG TG(18:0_18:2_18:2) TG 18:0_18:2_18:2 C57H102O6 882.7676 Glycerolipids Triradylglycerols TAG TG(18:0_16:1_20:3) TG 16:1_18:0_20:3 C57H102O6 882.7676 Glycerolipids Triradylglycerols TAG TG(16:0_18:0_20:3) TG 16:0_18:0_20:3 C57H104O6 884.7833 Glycerolipids Triradylglycerols TAG TG(18:0_18:1_18:2) TG 18:0_18:1_18:2 C57H104O6 884.7833 Glycerolipids Triradylglycerols TAG TG(18:0_18:0_18:2) TG 18:0_18:0_18:2 C57H106O6 886.7989 Glycerolipids Triradylglycerols TAG TG(18:0_18:1_18:1) TG 18:0_18:1_18:1 C57H106O6 886.7989 Glycerolipids Triradylglycerols TAG TG(18:0_18:0_18:1) TG 18:0_18:0_18:1 C57H108O6 888.8146 Glycerolipids Triradylglycerols TAG TG(16:0_20:4_20:4) TG 16:0_20:4_20:4 C59H98O6 902.7363 Glycerolipids Triradylglycerols TAG TG(18:1_18:2_20:5) TG 18:1_18:2_20:5 C59H98O6 902.7363 Glycerolipids Triradylglycerols TAG TG(18:0_18:2_20:5) TG 18:0_18:2_20:5 C59H100O6 904.752 Glycerolipids Triradylglycerols TAG TG(18:0_18:0_20:5) TG 18:0_18:0_20:5 C59H104O6 908.7833 Glycerolipids Triradylglycerols TAG TG(18:0_18:1_20:4) TG 18:0_18:1_20:4 C59H104O6 908.7833 Glycerolipids Triradylglycerols TAG TG(18:0_18:2_20:3) TG 18:0_18:2_20:3 C59H104O6 908.7833 Glycerolipids Triradylglycerols TAG TG(18:0_18:1_20:3) TG 18:0_18:1_20:3 C59H106O6 910.7989 Glycerolipids Triradylglycerols TAG TG(18:1_20:5_20:5) TG 18:1_20:5_20:5 C61H96O6 924.7207 Glycerolipids Triradylglycerols TAG TG(18:0_20:5_20:5) TG 18:0_20:5_20:5 C61H98O6 926.7363 Glycerolipids Triradylglycerols TAG TG(18:1_20:4_20:5) TG 18:1_20:4_20:5 C61H98O6 926.7363 Glycerolipids Triradylglycerols TAG TG(18:0_20:4_20:5) TG 18:0_20:4_20:5 C61H100O6 928.752 Glycerolipids Triradylglycerols TAG TG(18:1_20:3_20:5) TG 18:1_20:3_20:5 C61H100O6 928.752 Glycerolipids Triradylglycerols TAG TG(18:0_20:3_20:5) TG 18:0_20:3_20:5 C61H102O6 930.7676 Glycerolipids Triradylglycerols TAG TG(18:0_20:4_20:4) TG 18:0_20:4_20:4 C61H102O6 930.7676 Glycerolipids Triradylglycerols TAG METABOLITES_END #END