#METABOLOMICS WORKBENCH YuichiAbe_20221026_024847 DATATRACK_ID:3537 STUDY_ID:ST002346 ANALYSIS_ID:AN003830 PROJECT_ID:PR001506 VERSION 1 CREATED_ON November 15, 2022, 12:37 am #PROJECT PR:PROJECT_TITLE Lipidomics study of peroxisomal dysmorphogenesis PR:PROJECT_SUMMARY Proliferation of peroxisomes is accompanied by the growth and division of PR:PROJECT_SUMMARY pre-existing peroxisomes. Pex11β induces the elongation of peroxisome membrane PR:PROJECT_SUMMARY and then dynamin-like GTPase, DLP1, elicits the peroxisomal division. Nucleoside PR:PROJECT_SUMMARY diphosphate kinase 3 (NME3) generates GTP for the DLP1 activity. Deficiencies of PR:PROJECT_SUMMARY either of the factors induce abnormal morphology of peroxisomes. In this study, PR:PROJECT_SUMMARY we assessed the phospholipid compositions in cells lacking each of the different PR:PROJECT_SUMMARY division factors. In the fibroblasts from the patients deficient in DLP1, NME3, PR:PROJECT_SUMMARY or Pex11β, docosahexaenoic acid (DHA, C22:6)-containing phospholipids were PR:PROJECT_SUMMARY found to be decreased. Conversely, the levels of several fatty acids such as PR:PROJECT_SUMMARY arachidonic acid (AA, C20:4) and oleic acid (C18:1) were elevated. Mouse PR:PROJECT_SUMMARY embryonic fibroblasts from Drp1- and Pex11β-knockout mice also showed a PR:PROJECT_SUMMARY decrease in the levels of phospholipids containing DHA and AA. PR:INSTITUTE Kyushu university PR:LAST_NAME Abe PR:FIRST_NAME Yuichi PR:ADDRESS 3-1-1 Maidashi, Fukuoka 812-8582, Japan. PR:EMAIL y-abe@bio.sojo-u.ac.jp PR:PHONE +81-92-642-6341 #STUDY ST:STUDY_TITLE Lipidomics study of the cells defective in peroxisome division ST:STUDY_SUMMARY Proliferation of peroxisomes is accompanied by the growth and division of ST:STUDY_SUMMARY pre-existing peroxisomes. Pex11β induces the elongation of peroxisome membrane ST:STUDY_SUMMARY and then dynamin-like GTPase, DLP1, elicits the peroxisomal division. Nucleoside ST:STUDY_SUMMARY diphosphate kinase 3 (NME3) generates GTP for the DLP1 activity. Deficiencies of ST:STUDY_SUMMARY either of the factors induce abnormal morphology of peroxisomes. In this study, ST:STUDY_SUMMARY we assessed the phospholipid compositions in cells lacking each of the different ST:STUDY_SUMMARY division factors. In the fibroblasts from the patients deficient in DLP1, NME3, ST:STUDY_SUMMARY or Pex11β, docosahexaenoic acid (DHA, C22:6)-containing phospholipids were ST:STUDY_SUMMARY found to be decreased. Conversely, the levels of several fatty acids such as ST:STUDY_SUMMARY arachidonic acid (AA, C20:4) and oleic acid (C18:1) were elevated. Mouse ST:STUDY_SUMMARY embryonic fibroblasts from Drp1- and Pex11β-knockout mice also showed a ST:STUDY_SUMMARY decrease in the levels of phospholipids containing DHA and AA. ST:INSTITUTE Kyushu university ST:LAST_NAME Abe ST:FIRST_NAME Yuichi ST:ADDRESS 3-1-1 Maidashi, Fukuoka 812-8582, Japan. ST:EMAIL y-abe@bio.sojo-u.ac.jp ST:PHONE +81926426341 #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606/10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - FContA-1-PE Genotype:Control1 RAW_FILE_NAME=FContA-1-PE.mzML SUBJECT_SAMPLE_FACTORS - FContA-2-PE Genotype:Control1 RAW_FILE_NAME=FContA-2-PE.mzML SUBJECT_SAMPLE_FACTORS - FContA-3-PE Genotype:Control1 RAW_FILE_NAME=FContA-3-PE.mzML SUBJECT_SAMPLE_FACTORS - FContA-4-PE Genotype:Control1 RAW_FILE_NAME=FContA-4-PE.mzML SUBJECT_SAMPLE_FACTORS - FContA-5-PE Genotype:Control1 RAW_FILE_NAME=FContA-5-PE.mzML SUBJECT_SAMPLE_FACTORS - FContA-6-PE Genotype:Control1 RAW_FILE_NAME=FContA-6-PE.mzML SUBJECT_SAMPLE_FACTORS - FContB-1-PE Genotype:Control2 RAW_FILE_NAME=FContB-1-PE.mzML SUBJECT_SAMPLE_FACTORS - FContB-2-PE Genotype:Control2 RAW_FILE_NAME=FContB-2-PE.mzML SUBJECT_SAMPLE_FACTORS - FContB-3-PE Genotype:Control2 RAW_FILE_NAME=FContB-3-PE.mzML SUBJECT_SAMPLE_FACTORS - FContB-4-PE Genotype:Control2 RAW_FILE_NAME=FContB-4-PE.mzML SUBJECT_SAMPLE_FACTORS - FContB-5-PE Genotype:Control2 RAW_FILE_NAME=FContB-5-PE.mzML SUBJECT_SAMPLE_FACTORS - FContB-6-PE Genotype:Control2 RAW_FILE_NAME=FContB-6-PE.mzML SUBJECT_SAMPLE_FACTORS - FContC-1-PE Genotype:Control3 RAW_FILE_NAME=FContC-1-PE.mzML SUBJECT_SAMPLE_FACTORS - FContC-2-PE Genotype:Control3 RAW_FILE_NAME=FContC-2-PE.mzML SUBJECT_SAMPLE_FACTORS - FContC-3-PE Genotype:Control3 RAW_FILE_NAME=FContC-3-PE.mzML SUBJECT_SAMPLE_FACTORS - FContC-4-PE Genotype:Control3 RAW_FILE_NAME=FContC-4-PE.mzML SUBJECT_SAMPLE_FACTORS - FContC-5-PE Genotype:Control3 RAW_FILE_NAME=FContC-5-PE.mzML SUBJECT_SAMPLE_FACTORS - FContC-6-PE Genotype:Control3 RAW_FILE_NAME=FContC-6-PE.mzML SUBJECT_SAMPLE_FACTORS - FContD-1-PE Genotype:Control4 RAW_FILE_NAME=FContD-1-PE.mzML SUBJECT_SAMPLE_FACTORS - FContD-2-PE Genotype:Control4 RAW_FILE_NAME=FContD-2-PE.mzML SUBJECT_SAMPLE_FACTORS - FContD-3-PE Genotype:Control4 RAW_FILE_NAME=FContD-3-PE.mzML SUBJECT_SAMPLE_FACTORS - FContD-4-PE Genotype:Control4 RAW_FILE_NAME=FContD-4-PE.mzML SUBJECT_SAMPLE_FACTORS - FContD-5-PE Genotype:Control4 RAW_FILE_NAME=FContD-5-PE.mzML SUBJECT_SAMPLE_FACTORS - FContD-6-PE Genotype:Control4 RAW_FILE_NAME=FContD-6-PE.mzML SUBJECT_SAMPLE_FACTORS - FNME3-1-PE Genotype:dNME3 RAW_FILE_NAME=FNME3-1-PE.mzML SUBJECT_SAMPLE_FACTORS - FNME3-2-PE Genotype:dNME3 RAW_FILE_NAME=FNME3-2-PE.mzML SUBJECT_SAMPLE_FACTORS - FNME3-3-PE Genotype:dNME3 RAW_FILE_NAME=FNME3-3-PE.mzML SUBJECT_SAMPLE_FACTORS - FNME3-4-PE Genotype:dNME3 RAW_FILE_NAME=FNME3-4-PE.mzML SUBJECT_SAMPLE_FACTORS - FNME3-5-PE Genotype:dNME3 RAW_FILE_NAME=FNME3-5-PE.mzML SUBJECT_SAMPLE_FACTORS - FNME3-6-PE Genotype:dNME3 RAW_FILE_NAME=FNME3-6-PE.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-1-PE Genotype:DLP1mut RAW_FILE_NAME=FDLP1-1-PE.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-2-PE Genotype:DLP1mut RAW_FILE_NAME=FDLP1-2-PE.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-3-PE Genotype:DLP1mut RAW_FILE_NAME=FDLP1-3-PE.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-4-PE Genotype:DLP1mut RAW_FILE_NAME=FDLP1-4-PE.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-5-PE Genotype:DLP1mut RAW_FILE_NAME=FDLP1-5-PE.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-6-PE Genotype:DLP1mut RAW_FILE_NAME=FDLP1-6-PE.mzML SUBJECT_SAMPLE_FACTORS - FP11b-1-PE Genotype:dPEX11b RAW_FILE_NAME=FP11b-1-PE.mzML SUBJECT_SAMPLE_FACTORS - FP11b-2-PE Genotype:dPEX11b RAW_FILE_NAME=FP11b-2-PE.mzML SUBJECT_SAMPLE_FACTORS - FP11b-3-PE Genotype:dPEX11b RAW_FILE_NAME=FP11b-3-PE.mzML SUBJECT_SAMPLE_FACTORS - FP11b-4-PE Genotype:dPEX11b RAW_FILE_NAME=FP11b-4-PE.mzML SUBJECT_SAMPLE_FACTORS - FP11b-5-PE Genotype:dPEX11b RAW_FILE_NAME=FP11b-5-PE.mzML SUBJECT_SAMPLE_FACTORS - FP11b-6-PE Genotype:dPEX11b RAW_FILE_NAME=FP11b-6-PE.mzML SUBJECT_SAMPLE_FACTORS - MContA-1-PE Genotype:Wild-Type RAW_FILE_NAME=MContA-1-PE.mzML SUBJECT_SAMPLE_FACTORS - MContA-2-PE Genotype:Wild-Type RAW_FILE_NAME=MContA-2-PE.mzML SUBJECT_SAMPLE_FACTORS - MContA-3-PE Genotype:Wild-Type RAW_FILE_NAME=MContA-3-PE.mzML SUBJECT_SAMPLE_FACTORS - MContA-4-PE Genotype:Wild-Type RAW_FILE_NAME=MContA-4-PE.mzML SUBJECT_SAMPLE_FACTORS - MContA-5-PE Genotype:Wild-Type RAW_FILE_NAME=MContA-5-PE.mzML SUBJECT_SAMPLE_FACTORS - MDRP1-1-PE Genotype:Drp1-KO RAW_FILE_NAME=MDRP1-1-PE.mzML SUBJECT_SAMPLE_FACTORS - MDRP1-2-PE Genotype:Drp1-KO RAW_FILE_NAME=MDRP1-2-PE.mzML SUBJECT_SAMPLE_FACTORS - MDRP1-3-PE Genotype:Drp1-KO RAW_FILE_NAME=MDRP1-3-PE.mzML SUBJECT_SAMPLE_FACTORS - MDRP1-4-PE Genotype:Drp1-KO RAW_FILE_NAME=MDRP1-4-PE.mzML SUBJECT_SAMPLE_FACTORS - MDRP1-5-PE Genotype:Drp1-KO RAW_FILE_NAME=MDRP1-5-PE.mzML SUBJECT_SAMPLE_FACTORS - MP11b-1-PE Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-1-PE.mzML SUBJECT_SAMPLE_FACTORS - MP11b-2-PE Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-2-PE.mzML SUBJECT_SAMPLE_FACTORS - MP11b-3-PE Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-3-PE.mzML SUBJECT_SAMPLE_FACTORS - MP11b-4-PE Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-4-PE.mzML SUBJECT_SAMPLE_FACTORS - MP11b-5-PE Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-5-PE.mzML SUBJECT_SAMPLE_FACTORS - MP11b-6-PE Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-6-PE.mzML SUBJECT_SAMPLE_FACTORS - FContA-1-PC Genotype:Control1 RAW_FILE_NAME=FContA-1-PC.mzML SUBJECT_SAMPLE_FACTORS - FContA-2-PC Genotype:Control1 RAW_FILE_NAME=FContA-2-PC.mzML SUBJECT_SAMPLE_FACTORS - FContA-3-PC Genotype:Control1 RAW_FILE_NAME=FContA-3-PC.mzML SUBJECT_SAMPLE_FACTORS - FContA-4-PC Genotype:Control1 RAW_FILE_NAME=FContA-4-PC.mzML SUBJECT_SAMPLE_FACTORS - FContA-5-PC Genotype:Control1 RAW_FILE_NAME=FContA-5-PC.mzML SUBJECT_SAMPLE_FACTORS - FContA-6-PC Genotype:Control1 RAW_FILE_NAME=FContA-6-PC.mzML SUBJECT_SAMPLE_FACTORS - FContB-1-PC Genotype:Control2 RAW_FILE_NAME=FContB-1-PC.mzML SUBJECT_SAMPLE_FACTORS - FContB-2-PC Genotype:Control2 RAW_FILE_NAME=FContB-2-PC.mzML SUBJECT_SAMPLE_FACTORS - FContB-3-PC Genotype:Control2 RAW_FILE_NAME=FContB-3-PC.mzML SUBJECT_SAMPLE_FACTORS - FContB-4-PC Genotype:Control2 RAW_FILE_NAME=FContB-4-PC.mzML SUBJECT_SAMPLE_FACTORS - FContB-5-PC Genotype:Control2 RAW_FILE_NAME=FContB-5-PC.mzML SUBJECT_SAMPLE_FACTORS - FContB-6-PC Genotype:Control2 RAW_FILE_NAME=FContB-6-PC.mzML SUBJECT_SAMPLE_FACTORS - FContC-1-PC Genotype:Control3 RAW_FILE_NAME=FContC-1-PC.mzML SUBJECT_SAMPLE_FACTORS - FContC-2-PC Genotype:Control3 RAW_FILE_NAME=FContC-2-PC.mzML SUBJECT_SAMPLE_FACTORS - FContC-3-PC Genotype:Control3 RAW_FILE_NAME=FContC-3-PC.mzML SUBJECT_SAMPLE_FACTORS - FContC-4-PC Genotype:Control3 RAW_FILE_NAME=FContC-4-PC.mzML SUBJECT_SAMPLE_FACTORS - FContC-5-PC Genotype:Control3 RAW_FILE_NAME=FContC-5-PC.mzML SUBJECT_SAMPLE_FACTORS - FContC-6-PC Genotype:Control3 RAW_FILE_NAME=FContC-6-PC.mzML SUBJECT_SAMPLE_FACTORS - FContD-1-PC Genotype:Control4 RAW_FILE_NAME=FContD-1-PC.mzML SUBJECT_SAMPLE_FACTORS - FContD-2-PC Genotype:Control4 RAW_FILE_NAME=FContD-2-PC.mzML SUBJECT_SAMPLE_FACTORS - FContD-3-PC Genotype:Control4 RAW_FILE_NAME=FContD-3-PC.mzML SUBJECT_SAMPLE_FACTORS - FContD-4-PC Genotype:Control4 RAW_FILE_NAME=FContD-4-PC.mzML SUBJECT_SAMPLE_FACTORS - FContD-5-PC Genotype:Control4 RAW_FILE_NAME=FContD-5-PC.mzML SUBJECT_SAMPLE_FACTORS - FContD-6-PC Genotype:Control4 RAW_FILE_NAME=FContD-6-PC.mzML SUBJECT_SAMPLE_FACTORS - FNME3-1-PC Genotype:dNME3 RAW_FILE_NAME=FNME3-1-PC.mzML SUBJECT_SAMPLE_FACTORS - FNME3-2-PC Genotype:dNME3 RAW_FILE_NAME=FNME3-2-PC.mzML SUBJECT_SAMPLE_FACTORS - FNME3-3-PC Genotype:dNME3 RAW_FILE_NAME=FNME3-3-PC.mzML SUBJECT_SAMPLE_FACTORS - FNME3-4-PC Genotype:dNME3 RAW_FILE_NAME=FNME3-4-PC.mzML SUBJECT_SAMPLE_FACTORS - FNME3-5-PC Genotype:dNME3 RAW_FILE_NAME=FNME3-5-PC.mzML SUBJECT_SAMPLE_FACTORS - FNME3-6-PC Genotype:dNME3 RAW_FILE_NAME=FNME3-6-PC.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-1-PC Genotype:DLP1mut RAW_FILE_NAME=FDLP1-1-PC.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-2-PC Genotype:DLP1mut RAW_FILE_NAME=FDLP1-2-PC.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-3-PC Genotype:DLP1mut RAW_FILE_NAME=FDLP1-3-PC.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-4-PC Genotype:DLP1mut RAW_FILE_NAME=FDLP1-4-PC.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-5-PC Genotype:DLP1mut RAW_FILE_NAME=FDLP1-5-PC.mzML SUBJECT_SAMPLE_FACTORS - FDLP1-6-PC Genotype:DLP1mut RAW_FILE_NAME=FDLP1-6-PC.mzML SUBJECT_SAMPLE_FACTORS - FP11b-1-PC Genotype:dPEX11b RAW_FILE_NAME=FP11b-1-PC.mzML SUBJECT_SAMPLE_FACTORS - FP11b-2-PC Genotype:dPEX11b RAW_FILE_NAME=FP11b-2-PC.mzML SUBJECT_SAMPLE_FACTORS - FP11b-3-PC Genotype:dPEX11b RAW_FILE_NAME=FP11b-3-PC.mzML SUBJECT_SAMPLE_FACTORS - FP11b-4-PC Genotype:dPEX11b RAW_FILE_NAME=FP11b-4-PC.mzML SUBJECT_SAMPLE_FACTORS - FP11b-5-PC Genotype:dPEX11b RAW_FILE_NAME=FP11b-5-PC.mzML SUBJECT_SAMPLE_FACTORS - FP11b-6-PC Genotype:dPEX11b RAW_FILE_NAME=FP11b-6-PC.mzML SUBJECT_SAMPLE_FACTORS - MContA-1-PC Genotype:Wild-Type RAW_FILE_NAME=MContA-1-PC.mzML SUBJECT_SAMPLE_FACTORS - MContA-2-PC Genotype:Wild-Type RAW_FILE_NAME=MContA-2-PC.mzML SUBJECT_SAMPLE_FACTORS - MContA-3-PC Genotype:Wild-Type RAW_FILE_NAME=MContA-3-PC.mzML SUBJECT_SAMPLE_FACTORS - MContA-4-PC Genotype:Wild-Type RAW_FILE_NAME=MContA-4-PC.mzML SUBJECT_SAMPLE_FACTORS - MContA-5-PC Genotype:Wild-Type RAW_FILE_NAME=MContA-5-PC.mzML SUBJECT_SAMPLE_FACTORS - MDRP1-1-PC Genotype:Drp1-KO RAW_FILE_NAME=MDRP1-1-PC.mzML SUBJECT_SAMPLE_FACTORS - MDRP1-2-PC Genotype:Drp1-KO RAW_FILE_NAME=MDRP1-2-PC.mzML SUBJECT_SAMPLE_FACTORS - MDRP1-3-PC Genotype:Drp1-KO RAW_FILE_NAME=MDRP1-3-PC.mzML SUBJECT_SAMPLE_FACTORS - MDRP1-4-PC Genotype:Drp1-KO RAW_FILE_NAME=MDRP1-4-PC.mzML SUBJECT_SAMPLE_FACTORS - MDRP1-5-PC Genotype:Drp1-KO RAW_FILE_NAME=MDRP1-5-PC.mzML SUBJECT_SAMPLE_FACTORS - MP11b-1-PC Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-1-PC.mzML SUBJECT_SAMPLE_FACTORS - MP11b-2-PC Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-2-PC.mzML SUBJECT_SAMPLE_FACTORS - MP11b-3-PC Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-3-PC.mzML SUBJECT_SAMPLE_FACTORS - MP11b-4-PC Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-4-PC.mzML SUBJECT_SAMPLE_FACTORS - MP11b-5-PC Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-5-PC.mzML SUBJECT_SAMPLE_FACTORS - MP11b-6-PC Genotype:Pex11b-KO RAW_FILE_NAME=MP11b-6-PC.mzML #COLLECTION CO:COLLECTION_SUMMARY Fibroblasts and MEFs were cultured under 5% CO2 at 37°C in Dulbecco's modified CO:COLLECTION_SUMMARY medium (DMEM; Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine CO:COLLECTION_SUMMARY serum. For the extraction of phospholipids, fibroblasts were seeded in 6-well CO:COLLECTION_SUMMARY plate and cultured overnight, followed by replacement of fresh culture medium. CO:COLLECTION_SUMMARY Cells were harvested at 48 hours after the medium change as described below. CO:SAMPLE_TYPE Cultured fibroblasts #TREATMENT TR:TREATMENT_SUMMARY No treatment #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Cells were detached by incubation with trypsin and suspended in PBS. Protein SP:SAMPLEPREP_SUMMARY concentration was determined by the bicinchonic acid method (Thermo Fisher SP:SAMPLEPREP_SUMMARY Scientific, Rockford, IL). Total lipids were extracted from 50 μg of cellular SP:SAMPLEPREP_SUMMARY protein by the Bligh and Dyer method. Cells were dissolved in SP:SAMPLEPREP_SUMMARY methanol/chloroform/water at 2:1:0.8 v/v/v and then 50 pmol of SP:SAMPLEPREP_SUMMARY 1,2-adidodecanoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids, Alabaster, SP:SAMPLEPREP_SUMMARY AL) and 1,2-didodecanoyl-sn-glycero-3-phosphoethanolamine (Avanti Polar Lipids) SP:SAMPLEPREP_SUMMARY were added as internal standards. After incubation for 5 min at room SP:SAMPLEPREP_SUMMARY temperature, 1 ml each of water and chloroform were added and the samples were SP:SAMPLEPREP_SUMMARY then centrifuged at 2,000 rpm for 5 min in Himac CF-16RX (Hitachi Koki, Tokyo, SP:SAMPLEPREP_SUMMARY Japan) to collect the lower organic phase. To re-extract the lipids from the SP:SAMPLEPREP_SUMMARY water phase, 1 ml chloroform was added. The combined organic phase was SP:SAMPLEPREP_SUMMARY evaporated under a nitrogen stream and the extracted lipids were dissolved in SP:SAMPLEPREP_SUMMARY methanol. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY PE and pPE detection method CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Waters Aquity BEH C18 (150 × 1.0 mm, 1.7 μm) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 4000 QTrap MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS MS were detected by neutral loss, precoursour ion scan, and MRM. Peaks were MS:MS_COMMENTS detected by quantification wizard on Ananlyst software (version 1.42). #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS The ratio to total amount of each phospholipid species MS_METABOLITE_DATA_START Samples FContA-1-PE FContA-2-PE FContA-3-PE FContA-4-PE FContA-5-PE FContA-6-PE FContB-1-PE FContB-2-PE FContB-3-PE FContB-4-PE FContB-5-PE FContB-6-PE FContC-1-PE FContC-2-PE FContC-3-PE FContC-4-PE FContC-5-PE FContC-6-PE FContD-1-PE FContD-2-PE FContD-3-PE FContD-4-PE FContD-5-PE FContD-6-PE FNME3-1-PE FNME3-2-PE FNME3-3-PE FNME3-4-PE FNME3-5-PE FNME3-6-PE FDLP1-1-PE FDLP1-2-PE FDLP1-3-PE FDLP1-4-PE FDLP1-5-PE FDLP1-6-PE FP11b-1-PE FP11b-2-PE FP11b-3-PE FP11b-4-PE FP11b-5-PE FP11b-6-PE MContA-1-PE MContA-2-PE MContA-3-PE MContA-4-PE MContA-5-PE MDRP1-1-PE MDRP1-2-PE MDRP1-3-PE MDRP1-4-PE MDRP1-5-PE MP11b-1-PE MP11b-2-PE MP11b-3-PE MP11b-4-PE MP11b-5-PE MP11b-6-PE Factors Genotype:Control1 Genotype:Control1 Genotype:Control1 Genotype:Control1 Genotype:Control1 Genotype:Control1 Genotype:Control2 Genotype:Control2 Genotype:Control2 Genotype:Control2 Genotype:Control2 Genotype:Control2 Genotype:Control3 Genotype:Control3 Genotype:Control3 Genotype:Control3 Genotype:Control3 Genotype:Control3 Genotype:Control4 Genotype:Control4 Genotype:Control4 Genotype:Control4 Genotype:Control4 Genotype:Control4 Genotype:dNME3 Genotype:dNME3 Genotype:dNME3 Genotype:dNME3 Genotype:dNME3 Genotype:dNME3 Genotype:DLP1mut Genotype:DLP1mut Genotype:DLP1mut Genotype:DLP1mut Genotype:DLP1mut Genotype:DLP1mut Genotype:dPEX11b Genotype:dPEX11b Genotype:dPEX11b Genotype:dPEX11b Genotype:dPEX11b Genotype:dPEX11b Genotype:Wild-Type Genotype:Wild-Type Genotype:Wild-Type Genotype:Wild-Type Genotype:Wild-Type Genotype:Drp1-KO Genotype:Drp1-KO Genotype:Drp1-KO Genotype:Drp1-KO Genotype:Drp1-KO Genotype:Pex11b-KO Genotype:Pex11b-KO Genotype:Pex11b-KO Genotype:Pex11b-KO Genotype:Pex11b-KO Genotype:Pex11b-KO pPE 16:0/16:1 0.009199669 0 0 0.0106178 0.009201564 0.004080601 0.01007318 0.007334277 0.003769384 0.006268897 0.004976198 0.005938363 0.00498274 0.006667689 0.003209361 0.001467944 0.0061567 0.004261903 0.009084792 0.005651857 0.006488486 0.007870342 0.008136697 0.007259626 0.003741202 0 0.00893286 0.019548134 0.009378046 0.01571995 0.002660653 0 0 0.005114552 0.012813391 0.00719962 0.009882664 0.006873505 0.007367141 0.011682878 0.015496839 0.009931608 0.010680863 0.00688388 0.006282526 0.004249018 0.004555764 0.005603931 0.005160814 0.005464959 0.003923213 0.004463314 0.005711577 0.006140401 0.006201272 0.007102488 0.006908348 0.005055755 pPE 16:0/16:0 0.001294326 0.00282964 0.001301316 0.00495209 0.005325905 0.005170307 0.003927341 0.006875885 0.006223866 0.00310671 0.00658699 0.003834486 0.005700973 0.006782649 0.007954826 0.002899189 0.00374078 0.008008035 0.004275196 0.003456961 0.004271586 0.002916656 0.003393251 0.005485051 0.007599317 0.014495178 0.005958319 0.003929273 0.003119516 0.003187823 0.001497337 0.001694063 0.001428281 0.002659567 0.002202563 0.003388056 0.00263097 0.000894114 0.003083919 0.00186305 0.002596436 0.002673894 0.002762292 0.002732532 0.00374037 0.004059705 0.004077078 0.001526501 0.000815578 0.001232686 0.001560287 0.002533747 0.001327171 0.00125664 0.001595216 0.002750049 0.001515072 0.001853252 pPE 16:0/18:2 0.009000542 0.002212264 0.000974986 0.009212185 0.007576288 0.00744246 0.005756103 0.002758949 0.004704425 0.006268897 0.003221585 0.003800552 0.000718233 0.006706009 0.006269814 0.0029313 0.006858096 0.005021988 0.002300347 0.004389792 0.003460526 0.002297 0.003379039 0.002589267 0.00353076 0 0.008581828 0.003929273 0.015383116 0.018788229 0.002234488 0.001208979 0.001428281 0.005427108 0.007928111 0.006927366 0.003668815 0.004889689 0.003891612 0.007374574 0.005847092 0.003686985 0.005775702 0.003450698 0.001975433 0.002776586 0.002178843 0.003600716 0.002538105 0.002717129 0.003005397 0.003184193 0.003199431 0.0021896 0.002683344 0.002918949 0.00275381 0.003454503 pPE 16:0/18:1 0.027081276 0.033955683 0.035035421 0.113314203 0.108018363 0.108970599 0.084542765 0.091964956 0.071296873 0.079886827 0.085141879 0.079065064 0.062396473 0.072041692 0.059563229 0.063305075 0.058060016 0.067412274 0.066799943 0.069962303 0.076239706 0.059472723 0.081011651 0.060228006 0.037879674 0.11016335 0.066973358 0.104616896 0.127705206 0.117550955 0.059317583 0.060448934 0.057900317 0.115645698 0.115850918 0.140664846 0.124607503 0.13272012 0.119930196 0.12265081 0.116941848 0.107952262 0.072991479 0.083552432 0.040318271 0.044383303 0.041596102 0.04234645 0.048900831 0.045285475 0.043191336 0.04980405 0.056997275 0.057976809 0.056202328 0.064528702 0.059554721 0.065100065 pPE 16:0/20:5 0.057746838 0.019207255 0.023123378 0.013104658 0.016377784 0.020124783 0.032977674 0.022461223 0.022499423 0.024077002 0.020738951 0.025042926 0.027203067 0.035752606 0.028880329 0.03380858 0.039745783 0.030267658 0.00874472 0.013937588 0.009300163 0.011395971 0.011050273 0.017611314 0.01543246 0.036683949 0.030392048 0.003929273 0.006239033 0.012731366 0.029255662 0.022612379 0.030433373 0.016679122 0.022528118 0.028737979 0.019500909 0.02877931 0.019237782 0.030895584 0.029480795 0.027469699 0.021093868 0.013697694 0.01262982 0.013903964 0.014459597 0.016685008 0.013028941 0.011636064 0.010689856 0.010042456 0.01256073 0.011709602 0.011198208 0.009311189 0.013435545 0.008767509 pPE 16:0/20:4 0.12863606 0.113014116 0.130131563 0.184459952 0.216536811 0.220259721 0.214654679 0.218022839 0.214767219 0.197220604 0.204800736 0.231765832 0.293128757 0.223789086 0.240604097 0.262853683 0.270427189 0.22531082 0.173436942 0.167635164 0.177622294 0.190526387 0.187961242 0.212142396 0.27357542 0.265373251 0.19953442 0.190815324 0.19496978 0.195652607 0.181984041 0.181346802 0.182380505 0.253454453 0.281950427 0.266506944 0.192034374 0.178822898 0.193356846 0.21269824 0.22979482 0.212915076 0.157701773 0.125065905 0.160949244 0.150608745 0.163413257 0.139717927 0.116245228 0.120044886 0.113737184 0.11457653 0.092902003 0.100912016 0.098882292 0.107403478 0.114821503 0.113925114 pPE 16:0/20:3 0.001123076 0.006636793 0.003693734 0.002832855 0.004100697 0.0054949 0.006385677 0.006102348 0.004295344 0.009098222 0.005004962 0.005090026 0.006374316 0.012798896 0.004741547 0.007247972 0.004792874 0.004094504 0.006752867 0.002935673 0.004271586 0.004095427 0.005436308 0.005027963 0.011317137 0.010726431 0.002854451 0.040275049 0.021836615 0.012731366 0.003547537 0.004350831 0.005548323 0.00605222 0.003573233 0.006685361 0.010345397 0.004498514 0.004185319 0.005938473 0.003904876 0.00508206 0.007734418 0.006533555 0.010281969 0.009108043 0.010481557 0.008241076 0.006582153 0.010100964 0.010851823 0.011294361 0.018011613 0.020087203 0.019649686 0.00887811 0.009290537 0.011235011 pPE 16:0/20:2 0 0.002195115 0.000641649 0.005319713 0.003675625 0.004173342 0.001442024 0 0.00046752 0.000443816 0 0.001082479 0 0 0.001253963 0.002183566 0.000623463 0 0.001561904 0.003539269 0.000865131 0.003952977 0.00447696 0.005108626 0 0 0 0 0 0 0.000374334 0.001455252 0.001067915 0.001815666 0.001778242 0.002858673 0.001031235 0.001791023 0.000783218 0.000621017 0.00130844 0 0.011618368 0.011770909 0.006687328 0.005237651 0.005727717 0.009230005 0.011218426 0.008489108 0.008530289 0.007838014 0.010961014 0.011424002 0.010986921 0.01312228 0.009147605 0.01002751 pPE 16:0/20:1 0.001294326 0.002212264 0.002602631 0.005319713 0.002372903 0.004799343 0.000983334 0.001406691 0.001878848 0.000898727 0.002301132 0.001096052 0 0.001226242 0 0.00072021 0.000623463 0.00144778 0.004518105 0.001305963 0.001286883 0.003952977 0.001137006 0.002137556 0 0 0 0 0 0 0.000374334 0.000486577 0.00035707 0.000454627 0 0.000974066 0.000528838 0.001341172 0.000381819 0 0.002612792 0.000531457 0.026953275 0.03380633 0.012370747 0.012347393 0.011504954 0.022593226 0.033672199 0.017039619 0.015008989 0.019649467 0.027254414 0.027132004 0.027784234 0.026547714 0.020343893 0.023572524 pPE 16:0/22:6 0.13062733 0.102210035 0.108109299 0.142723996 0.137773421 0.140966221 0.151097707 0.157285859 0.152528555 0.155890267 0.172872532 0.121821279 0.116712828 0.141017781 0.151259253 0.14908804 0.149241518 0.146587763 0.190926382 0.185194331 0.194384216 0.176281423 0.1844081 0.216982147 0.067224729 0.032112393 0.059860326 0.040275049 0.075063365 0.0619633 0.042270977 0.034702166 0.040211605 0.080980403 0.085143446 0.101036685 0.141133697 0.156470036 0.148321834 0.17155588 0.137795115 0.152793971 0.098438049 0.065685874 0.101200481 0.100756409 0.097717827 0.100414336 0.064298671 0.086579688 0.086562636 0.092532114 0.057826757 0.057215209 0.058526485 0.079686451 0.076230043 0.085050085 pPE 16:0/22:5 0.039825405 0.050762031 0.04744797 0.065090792 0.056259564 0.044515649 0.058160625 0.064174923 0.074511076 0.061856814 0.056665372 0.059044297 0.089779099 0.09350092 0.070535403 0.079819443 0.065073978 0.098630038 0.077002116 0.071882836 0.07272511 0.058760474 0.069996909 0.066949882 0.06500339 0.054301165 0.068543768 0.032170923 0.034119711 0.028491164 0.025685089 0.028582644 0.028345886 0.026709326 0.024873053 0.025259171 0.046603867 0.055882156 0.050174878 0.041918631 0.041297646 0.040523618 0.081864296 0.067262335 0.103629292 0.112115169 0.100688977 0.083932185 0.060745323 0.080746305 0.083143321 0.078380144 0.054745823 0.055406409 0.054828963 0.072757195 0.075753605 0.069300069 pPE 16:0/22:4 0.023218211 0.050247551 0.033834206 0.033302266 0.030755228 0.035241555 0.026022383 0.03094148 0.033018634 0.028570636 0.040557449 0.02779154 0.033083598 0.026977315 0.02950731 0.027065213 0.037018131 0.019997466 0.051253774 0.048013345 0.047852582 0.05662373 0.057916224 0.046784255 0.011387285 0.010860233 0.012009016 0.066797642 0.046012868 0.053993744 0.046302269 0.045821785 0.045595126 0.02707871 0.021188156 0.015367256 0.041646009 0.034646937 0.035734303 0.038813547 0.031893231 0.029329799 0.038002444 0.03608344 0.061853734 0.063104223 0.063219472 0.05223574 0.047377968 0.064167215 0.067126534 0.066949706 0.034364261 0.030940005 0.035918781 0.040449536 0.0436417 0.050557551 pPE 16:0/22:3 0 0.010512542 0.008438531 0.020889603 0.014752508 0.020124783 0.001963671 0.001406691 0.000958417 0.004465896 0.00277574 0.003800552 0.003591164 0 0.001222614 0.004316672 0.001827527 0.002117379 0.006582831 0.009438052 0.009029809 0.008012792 0.013573005 0.003818025 0.003799659 0 0 0.003929273 0 0.006375645 0.001382157 0.006029968 0.002702747 0.0018043 0.002199772 0.001433874 0.005784168 0.003576458 0.002193009 0.002476304 0.001958572 0.005779598 0.00934157 0.015729577 0.011658295 0.012389462 0.012825465 0.010396434 0.021150879 0.013815908 0.015512888 0.015594383 0.018367105 0.020658403 0.023452851 0.013252203 0.012244451 0.012285012 pPE 18:0/16:1 0.002588651 0.002778192 0.00419424 0.003784348 0.005575948 0.004103786 0.003447666 0.002300556 0.004207684 0.004909711 0.000471732 0.002120844 0.003573208 0.002425659 0.001253963 0.002912951 0.0061567 0.007872306 0.001552188 0.001314194 0.00302796 0.003422352 0.00055429 0.002984513 0 0.003623794 0 0.004027505 0.019165529 0.003187823 0.001485819 0.001455252 0.001779858 0.001329783 0.003991971 0.003388056 0.002650802 0.00310146 0.001563988 0.003741626 0.001954483 0.001587729 0.005340432 0.005097224 0.004161364 0.003849358 0.003614899 0.003499287 0.004280938 0.003622838 0.00453509 0.003755715 0.003566773 0.004512481 0.00420461 0.00485048 0.006003116 0.004551755 pPE 18:0/16:0 0.003878994 0.002229414 0.001621639 0.003546475 0.00282548 0.003037266 0.007255088 0.00415418 0.003360303 0.007988683 0.003681811 0.011978527 0.00141851 0.001210914 0.003083965 0.007293846 0.006078767 0.002891036 0.001535184 0.003429525 0.002162829 0.002261388 0.002803429 0.002578512 0 0.003623794 0.006004508 0 0.003119516 0.003187823 0.00187743 0.00072688 0.001067915 0.002270293 0.005276102 0.001452024 0.002650802 0.002679549 0.005090914 0.004230677 0.00258417 0.005812814 0.00373328 0.002627435 0.003853714 0.004585574 0.0043907 0.002109715 0.002859599 0.002318002 0.002465505 0.004463314 0.003009835 0.00168504 0.00315874 0.001113012 0.00229643 0.001349251 pPE 18:0/18:2 0.001210692 0.001548585 0.000645653 0.001416428 0.003600612 0.000373282 0.000491667 0.001836434 0.003301863 0.002241269 0.001852411 0.001635595 0.003555252 0 0.003130988 0.003642335 0.004325276 0.006469769 0.000777308 0.000438979 0.001286883 0.001680906 0.000568503 0 0 0 0 0.003831041 0 0 0.000580506 0.001365698 0.001790845 0.002258927 0.001334379 0.000974066 0.002108742 0.002671167 0.001559093 0.000621017 0.001275729 0.001062915 0.001975457 0.001574709 0.001306701 0.001283119 0.000907851 0.002340465 0.002555026 0.002118439 0.002339531 0.00260995 0.00138642 0.00178976 0.0012149 0.001927199 0.000647955 0.001223251 pPE 18:0/18:1 0.018359512 0.018006801 0.012212347 0.018986616 0.020478481 0.025040052 0.012261699 0.018307043 0.016275557 0.017253336 0.023068847 0.021683509 0.012479295 0.015059779 0.011559969 0.008257184 0.014027923 0.014839749 0.012728425 0.01168782 0.017275593 0.011075459 0.011050273 0.006157238 0.033787734 0.007247589 0.036027048 0.003929273 0.027880678 0.041242454 0.030752999 0.017910795 0.018457786 0.025686415 0.020462343 0.018785563 0.029780202 0.018748463 0.016031485 0.012420335 0.017582166 0.01889995 0.031640802 0.039586687 0.016013964 0.017164349 0.017991965 0.022720011 0.043655413 0.025022144 0.023395307 0.024194426 0.039341154 0.037604006 0.037292146 0.025551634 0.027204597 0.03034503 pPE 18:0/20:5 0.024930704 0.010735484 0.013513662 0.007784945 0.004100697 0.007604757 0.014720035 0.009397042 0.010665311 0.010457408 0.009434641 0.013098333 0.010279707 0.016937462 0.016693379 0.007523212 0.01593728 0.022983513 0.005173959 0.002696978 0.003839021 0.002916656 0.004654617 0.00483975 0.014263334 0.006690082 0.003002254 0.011689587 0.009573016 0.025303341 0.018198404 0.01768691 0.020655142 0.01415026 0.014934998 0.010406174 0.017319451 0.010533786 0.014832183 0.009315251 0.014392843 0.011160603 0.004051362 0.002855146 0.005877724 0.005532137 0.0043907 0.004260002 0.002876519 0.006769795 0.007648466 0.006123449 0.007382391 0.007444641 0.006169579 0.005716637 0.006574841 0.007035007 pPE 18:0/20:4 0.113502405 0.133250331 0.144145731 0.065090792 0.072012242 0.060745313 0.076148448 0.065320903 0.073342275 0.07600344 0.070759805 0.078386394 0.083045666 0.07012569 0.100708881 0.082571837 0.065853307 0.082342509 0.061941765 0.05569548 0.052178239 0.07407381 0.054007767 0.04920413 0.12041995 0.064559291 0.134870487 0.136787819 0.130824722 0.084875775 0.157796288 0.194779899 0.166999017 0.113940847 0.10328877 0.096196604 0.080647827 0.070970338 0.081748337 0.059772863 0.069102001 0.071746734 0.052232434 0.046242856 0.061529892 0.06499735 0.062724281 0.061364316 0.062775807 0.07214974 0.066226715 0.070215545 0.066240076 0.06502161 0.064548163 0.06236331 0.059554721 0.058275058 pPE 18:0/20:3 0.005018001 0.004853262 0.005996062 0.014207525 0.019653341 0.011012986 0.003777443 0.002346396 0.001370419 0.002726693 0.003221585 0.000519183 0.003546274 0 0 0 0 0 0.008404647 0.014376567 0.007461758 0.013995677 0.009309234 0.009249301 0 0.010860233 0 0 0 0 0.004169508 0.002731396 0.004032147 0.002327121 0.003098663 0.0014853 0.004329863 0.004582337 0.002447555 0.004347117 0.002616881 0.00584603 0.006579278 0.013189724 0.007496932 0.007467333 0.009227072 0.007962147 0.013265831 0.009379466 0.00912417 0.008844981 0.012205238 0.012185602 0.012677217 0.009397804 0.00857588 0.006562507 pPE 18:0/20:2 0 0.000564213 0.000659667 0.0017408 0.001612774 0.000370964 0.000482673 0 0 0.00044659 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.000491801 0.000724029 0 0 0 0 0 0 0 0 0 0.002946445 0.004536704 0.001515578 0.001920472 0.001462466 0.002206073 0.006429867 0.003576785 0.002753448 0.003592423 0.006007821 0.005940481 0.00590547 0.00336502 0.003978255 0.004588505 pPE 18:0/20:1 0.002588651 0 0.000650658 0.00106178 0.002020343 0.000380238 0 0 0.000470442 0 0 0.000546329 0 0 0 0 0 0.00144778 0.001156246 0 0.000432566 0.001705834 0 0.0004302 0 0 0 0 0 0 0.000374334 0.000723895 0.000715239 0.000909254 0 0.000968016 0.000528838 0 0.000741609 0 0 0 0.005474361 0.010299545 0.003011726 0.00363901 0.002393426 0.006871789 0.014721011 0.006861901 0.005956805 0.008219029 0.013864202 0.011804802 0.011515139 0.008748186 0.00719421 0.004488754 pPE 18:0/22:6 0.076066524 0.080087393 0.094795839 0.026598563 0.028754888 0.035241555 0.024553377 0.027675435 0.036817237 0.032176639 0.036242827 0.026128798 0.028684422 0.030119559 0.03272059 0.027799185 0.022990208 0.030358145 0.030606519 0.039508124 0.056774249 0.035006998 0.029100238 0.033878253 0.018939837 0.039805988 0.023740901 0.024140472 0.028660558 0.026100297 0.033056595 0.037015644 0.034718217 0.025572759 0.022918941 0.015851264 0.040984961 0.034646937 0.032062971 0.029226601 0.040847869 0.024480251 0.028292569 0.023646915 0.032222233 0.036179754 0.035818865 0.034485732 0.036548718 0.049430247 0.043551264 0.044633137 0.035312241 0.032653605 0.035918781 0.039496763 0.036971571 0.034282534 pPE 18:0/22:5 0.024930704 0.048704111 0.038038457 0.010271803 0.009426603 0.01138395 0.012771354 0.013150129 0.01005169 0.013619594 0.016021631 0.014998609 0.014768662 0.020079706 0.017437919 0.015780396 0.019132529 0.018504443 0.012339771 0.014376567 0.011057461 0.016452933 0.01570489 0.016724027 0.01543246 0.02207727 0.048682703 0.011714145 0.009573016 0.009483772 0.024994011 0.02037353 0.023841307 0.005910149 0.005024859 0.007441624 0.016294827 0.014641125 0.017646872 0.014166945 0.009772413 0.013685025 0.022600573 0.018216882 0.035622569 0.033234891 0.03515861 0.032457159 0.031134093 0.038838051 0.037612455 0.038917919 0.03069084 0.031130405 0.029263242 0.02542171 0.029586786 0.028875029 pPE 18:0/22:4 0.025806863 0.021093682 0.024624896 0.003546475 0.008201394 0.004266083 0.007225109 0.00891 0.014843775 0.008626668 0.012713754 0.010010384 0.007361886 0.011304414 0.005838764 0.004394657 0.008299855 0.005745878 0.009740646 0.013498609 0.007083263 0.010933009 0.010090925 0.014089051 0.030397269 0.02531081 0.012009016 0.00805501 0.015968025 0.016317667 0.02510919 0.025149742 0.027906415 0.007728656 0.006197326 0.004840081 0.01563378 0.018748463 0.015223792 0.007413388 0.010426633 0.014847588 0.013744496 0.011788425 0.018782809 0.019057475 0.019642604 0.021705725 0.024873433 0.031162547 0.025194946 0.025092532 0.014456689 0.011614402 0.011832069 0.019315303 0.021106193 0.018427518 pPE 18:1/16:1 0.00393475 0.003961496 0.00333337 0.006963201 0.008751488 0.007767053 0.002413366 0.008308361 0.004236904 0.006823666 0.005551481 0.005565095 0.002872931 0.006667689 0.004898292 0.005871775 0.006780163 0.002153573 0.003570761 0.003511833 0.002657576 0.004487164 0.006999691 0.002148311 0.015198634 0.007191838 0.005718139 0.003929273 0.009319555 0.006375645 0.003605127 0.004589641 0.002362157 0.00863791 0.008933083 0.005445091 0.007899521 0.004107338 0.005213292 0.00306627 0.006501313 0.003158849 0.009241123 0.009283604 0.004469013 0.004732817 0.003862495 0.005553217 0.007529713 0.006462775 0.00692861 0.004817113 0.011399455 0.009900802 0.009127596 0.01009073 0.007480073 0.008242508 pPE 18:1/16:0 0.00393475 0.010169555 0.016116294 0.014510273 0.013102227 0.019753819 0.025033053 0.019223827 0.027262288 0.022246262 0.020365017 0.024092788 0.024375025 0.020118026 0.02319831 0.018257551 0.02143155 0.026602964 0.014720278 0.017970709 0.017708159 0.019230701 0.016877427 0.015863627 0.007599317 0.011016335 0.02438754 0.035117878 0.021641646 0.028491164 0.011748337 0.014254008 0.016260431 0.019861509 0.015660811 0.01436899 0.00899025 0.016401413 0.013192322 0.013545928 0.014392843 0.017272362 0.016456565 0.017358587 0.019754334 0.02071922 0.021458307 0.012425006 0.009644801 0.010945269 0.013569278 0.01450577 0.012323735 0.014851202 0.013733652 0.021264156 0.016818253 0.016327516 pPE 18:1/18:2 0.002588651 0.001114707 0.001621639 0.003178853 0.00482582 0.001866411 0.001963671 0.000939704 0.004295344 0.002726693 0.00141232 0.001096052 0.002940265 0.001881514 0.000642656 0 0.00246268 0.002162622 0.002793452 0.001712019 0.001732966 0.000569799 0 0.00212949 0 0 0.005708901 0 0 0.013050148 0.000748669 0.002403032 0.001779858 0.002716395 0.002224896 0.002477516 0.000528838 0 0.003083919 0.004851693 0.001958572 0.00159105 0.003565868 0.004711867 0.001057343 0.000927632 0.002541984 0.001290679 0.002588868 0.002410108 0.00214157 0.001660135 0.00324683 0.004369681 0.003929937 0.003754791 0.00229643 0.002866503 pPE 18:1/18:1 0.023616465 0.029325362 0.028729045 0.033734763 0.035255994 0.02874969 0.022574717 0.029509005 0.018671599 0.022246262 0.019789734 0.024466056 0.01692336 0.015059779 0.01540023 0.017294213 0.01165097 0.011446514 0.02188609 0.017970709 0.019925058 0.019836112 0.01222281 0.019816089 0.01543246 0.040363494 0.042585818 0.052308448 0.012770521 0.044430276 0.036051269 0.042314254 0.034388613 0.040916414 0.032382425 0.03660311 0.027598744 0.034646937 0.026923105 0.017272029 0.024737699 0.02398201 0.038337268 0.061131654 0.026069244 0.022507173 0.023274009 0.031189301 0.041624929 0.030241487 0.026814621 0.029120401 0.060552198 0.06235601 0.05747005 0.048504796 0.051931717 0.045517546 pPE 18:1/20:5 0.027559181 0.020064722 0.007417499 0.00637933 0.004925837 0.005309419 0.011302348 0.003237396 0.008970549 0.014091149 0.008744301 0.017272153 0.008663683 0.018087063 0.008189944 0.008027818 0.009858513 0.005700635 0.004785305 0.005158005 0.004758223 0.002852554 0.00397952 0.00169929 0.024434728 0.006489379 0.018290655 0 0.003119516 0.003187823 0.01520373 0.013955495 0.022413026 0.012161267 0.006616064 0.01736379 0.009386878 0.008522029 0.00802798 0.011644064 0.009118193 0.013253216 0.006997807 0.010282029 0.005829148 0.004648678 0.006305441 0.009255362 0.005566911 0.006600934 0.00653269 0.006558894 0.011257258 0.010472002 0.010669991 0.009744267 0.008099442 0.008085008 pPE 18:1/20:4 0.082836843 0.084203233 0.083083998 0.07957944 0.047007991 0.064223098 0.089939111 0.081078139 0.078017479 0.081828521 0.06213056 0.101800512 0.057458623 0.060928878 0.065441179 0.058717751 0.068191294 0.06288796 0.052954136 0.063377615 0.054341067 0.05662373 0.049033368 0.05458163 0.139125962 0.11016335 0.076118686 0.09651277 0.07974264 0.081488713 0.134760334 0.104479639 0.123051907 0.09831305 0.106359518 0.097709129 0.046603867 0.055323334 0.054580477 0.059384728 0.066239788 0.07407186 0.072321832 0.080924997 0.068978248 0.067100824 0.061898961 0.076325038 0.076989198 0.063092645 0.069106137 0.06477248 0.085199668 0.091296815 0.084726066 0.076654901 0.078135794 0.084525085 pPE 18:1/20:3 0.010513907 0.022637122 0.020720949 0.02551732 0.027504676 0.026894871 0.020116381 0.019710869 0.028226549 0.024409864 0.028246394 0.031422425 0.018359826 0.020692826 0.026294031 0.020275973 0.012780997 0.024069349 0.047610141 0.048562069 0.03406455 0.030021261 0.045835539 0.036567004 0.007599317 0.01471818 0.036488933 0.06237721 0.047962566 0.034866809 0.029255662 0.023507919 0.021753819 0.022504028 0.015074577 0.013884982 0.029780202 0.031014596 0.03646857 0.033030329 0.033365227 0.031389196 0.028794804 0.035207629 0.032060312 0.033445238 0.031692268 0.034992875 0.043993827 0.033004668 0.040491877 0.035379926 0.023936485 0.026084804 0.027044729 0.031787965 0.03368415 0.027037527 pPE 18:1/20:2 0 0.00050762 0.001151164 0.001416428 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.001261219 0 0 0.000446654 0 0 0 0 0 0 0 0.004436409 0.007234204 0.001975433 0.002503134 0.002442946 0.003879645 0.006328343 0.003929858 0.004913014 0.003156978 0.008685863 0.008101521 0.008293013 0.005023711 0.005193172 0.007927508 pPE 18:1/20:1 0 0.000557353 0.000650658 0.001416428 0 0.001898871 0 0 0 0.000890405 0.000463103 0.000536149 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.000485084 0.00035707 0.000454627 0 0.000484008 0 0 0 0 0.000641954 0.000518171 0.006395125 0.017866558 0.003788946 0.00363901 0.004291661 0.005857503 0.016091588 0.0053268 0.005920812 0.005034836 0.017300628 0.016564803 0.017959391 0.010307269 0.009957549 0.008662509 pPE 18:1/22:6 0.061729378 0.049218591 0.038138558 0.034383508 0.037256334 0.02874969 0.031178892 0.044120259 0.030681031 0.031621869 0.039406883 0.020665504 0.0279213 0.029506438 0.030800459 0.033074608 0.032536989 0.02221438 0.046881414 0.045818449 0.04163445 0.043803263 0.043703654 0.034684878 0.026539154 0.039917489 0.012009016 0.003929273 0.024566192 0.031878225 0.019004662 0.017612282 0.02417091 0.018526043 0.024314735 0.018876315 0.018939018 0.02746608 0.034755281 0.027868127 0.023224815 0.027104322 0.0416855 0.043790583 0.038699064 0.036179754 0.039120143 0.045896452 0.037225546 0.042829314 0.046970578 0.039462225 0.051901884 0.048742408 0.053878172 0.052835582 0.055743219 0.057225057 pPE 18:1/22:5 0.020987989 0.019207255 0.016516698 0.010271803 0.009976696 0.008346684 0.013041171 0.01188955 0.012915253 0.013786025 0.010067452 0.008754844 0.010683713 0.01578786 0.009326348 0.014450072 0.007481559 0.014975478 0.015108932 0.014815546 0.014599093 0.013995677 0.019258033 0.012771564 0.018939837 0.015387188 0.011824262 0 0.006395009 0.006375645 0.012093876 0.013507725 0.012524926 0.008922051 0.005276102 0.004295572 0.004726491 0.006202919 0.005409097 0.007374574 0.009036416 0.012157085 0.025446571 0.032755356 0.035298728 0.03007968 0.032682651 0.030175015 0.0313033 0.028706386 0.033473285 0.031841933 0.042422088 0.037889606 0.038665512 0.034213205 0.036352202 0.032182532 pPE 18:1/22:4 0.024333323 0.032583736 0.037337748 0.008152568 0.013552304 0.013285139 0.013940562 0.012806335 0.013908734 0.017253336 0.014784772 0.011876726 0.008843241 0.010652974 0.01026682 0.016147382 0.010832674 0.011853702 0.031335246 0.030454179 0.027576064 0.041310394 0.026186661 0.021563777 0.007599317 0.021742766 0.023925655 0.023575639 0.003119516 0.003187823 0.028449404 0.038582838 0.027357076 0.014718543 0.009268073 0.010950683 0.021120476 0.016932293 0.022835688 0.017698978 0.013411513 0.017239146 0.018080458 0.022595941 0.028983818 0.029658985 0.031692268 0.036514304 0.034010613 0.03699593 0.038152347 0.035924233 0.031283327 0.028084004 0.028629382 0.033390355 0.032445412 0.035752536 pPE 18:1/22:3 0.010036002 0.006602494 0.008748845 0.005319713 0.006851165 0.008230758 0.007375007 0.005042315 0.007188127 0.007572605 0.005925415 0.002171744 0.008573904 0.006092888 0.008111571 0.010000367 0.009001251 0.009320086 0.009157665 0.006282889 0.010624895 0.007585443 0.006182468 0.005915251 0.003799659 0.014495178 0.014965081 0.007858546 0.012770521 0.00978263 0.01007823 0.01104499 0.011755852 0.006307947 0.00488528 0.008712145 0.012097174 0.006873505 0.011919593 0.010518471 0.005887981 0.010463065 0.004670785 0.004939578 0.008063654 0.009907363 0.008566816 0.008139647 0.00939099 0.010883865 0.011337726 0.010260179 0.012797725 0.014851202 0.014261869 0.00645287 0.006527197 0.005460005 PE 14:0/18:2 0.010854307 0.004185073 0.004352149 0.006825779 0.008584301 0.008235214 0.00407047 0.004997057 0.004851275 0.005053313 0.005865515 0.004773677 0.009741099 0.009256879 0.008981724 0.009394902 0.00790286 0.009139266 0.007772298 0.011838815 0.01125611 0.00897084 0.010024658 0.013153531 0.005963568 0.00779707 0.005644858 0.007003663 0.008560748 0.009850174 0.008470378 0.005588837 0.005526789 0.006225115 0.006427965 0.007246922 0.007341484 0.0091157 0.008692872 0.009505729 0.009696759 0.008195664 0.026542642 0.03126766 0.003976651 0.003477634 0.004084445 0.015364891 0.015536224 0.004622002 0.004058359 0.005489044 0.004177938 0.00487997 0.003843686 0.002241057 0.002646431 0.002869284 PE 16:0/16:1 0.03073786 0.01436498 0.014330247 0.019280106 0.015180097 0.019638146 0.013729213 0.016190289 0.01415881 0.014775271 0.014661577 0.012920684 0.019260289 0.017441103 0.014697367 0.017616369 0.022349717 0.02242563 0.019732281 0.020949004 0.024073841 0.019605489 0.021102421 0.03052892 0.032829743 0.027972367 0.012878096 0.013929182 0.013158879 0.01233838 0.014386197 0.013891094 0.019361746 0.013606229 0.021431435 0.018684666 0.023238044 0.024287105 0.024896197 0.025810379 0.026126633 0.028295326 0.026303519 0.020711239 0.020300556 0.021021058 0.018783555 0.023767043 0.018872528 0.022335991 0.022834383 0.020250122 0.0181044 0.018499234 0.017213027 0.022246393 0.026334159 0.020700542 PE 16:0/16:0 0.003622676 0.003726977 0.004316766 0.003626681 0.003488759 0.00356475 0.003237979 0.004142412 0.003435805 0.002652179 0.002749322 0.002004216 0.005134547 0.005021979 0.005014796 0.003037561 0.004798931 0.005766814 0.003691701 0.005286316 0.005203101 0.005617496 0.004746264 0.004172357 0.00683702 0.001944717 0.00476514 0.01040293 0.002986916 0.008745673 0.003542769 0.002668872 0.002961208 0.00413236 0.002198848 0.003418097 0.006255244 0.006422133 0.004670265 0.005668805 0.00312481 0.00362719 0.002207104 0.001497405 0.003706631 0.004471244 0.003448543 0.001847804 0.001371868 0.003980672 0.003838328 0.005118163 0.003638288 0.002870571 0.003593011 0.002930613 0.004468564 0.001911201 PE 16:1/18:2 0.003622676 0.003659111 0.001822241 0.007074555 0.006835482 0.007727183 0.00676112 0.006584254 0.006707021 0.005749763 0.006807003 0.008173035 0.012013768 0.009681763 0.008505421 0.013145435 0.010084192 0.010015634 0.007270678 0.008736666 0.007877204 0.010377358 0.009526858 0.009334764 0.006776782 0.004914885 0.00158838 0.014700855 0.017084112 0.019585381 0.005875484 0.002134288 0.002325807 0.005015968 0.008135736 0.006244466 0.007281553 0.008228558 0.009388065 0.008047516 0.00746731 0.005964171 0.007962792 0.01006876 0.003338423 0.003819188 0.003668663 0.005791125 0.008614637 0.003781638 0.003227129 0.003267465 0.001683361 0.001200855 0.000733643 0.001563815 0.002221267 0.001097079 PE 16:0/18:2 0.03073786 0.030935607 0.020805395 0.039376506 0.036574269 0.041667022 0.02796436 0.03470905 0.033658548 0.032757453 0.032664774 0.035867651 0.034281324 0.033990648 0.035654723 0.038099482 0.040479807 0.043505409 0.03150627 0.03507328 0.036276598 0.034476844 0.03875114 0.038258388 0.024336177 0.046114966 0.050828157 0.031941392 0.02882243 0.038308513 0.019696989 0.027498696 0.029252421 0.033683388 0.043170709 0.039332476 0.043149946 0.039471367 0.044563598 0.043199576 0.041544748 0.038868544 0.044715982 0.048766075 0.030684033 0.028100528 0.026169796 0.036152685 0.042077273 0.030297334 0.030559934 0.029781773 0.044564676 0.040506943 0.040275141 0.033985265 0.035922049 0.030976351 PE 16:0/18:1 0.072727971 0.107454576 0.111457475 0.113115136 0.120286735 0.120817288 0.116824794 0.119475876 0.115624109 0.116614912 0.110061281 0.122855815 0.11860726 0.114927397 0.122478058 0.126998273 0.110139405 0.125977894 0.12568691 0.131683074 0.143724425 0.132933105 0.122733402 0.126585053 0.087345188 0.105882389 0.114363353 0.106471306 0.121121495 0.124410283 0.127055663 0.120281483 0.113892622 0.131544593 0.144390996 0.144799202 0.136341843 0.133714076 0.137850062 0.170428706 0.175490747 0.167159083 0.076041081 0.074296549 0.081005847 0.089424886 0.083645516 0.076991829 0.0824117 0.096641861 0.097547309 0.100508849 0.089477514 0.087552408 0.08890612 0.108769265 0.104555723 0.106729414 PE 16:0/18:0 0.002717007 0.001538297 0.003018198 0.001846381 0.001890854 0.002796301 0.003164388 0.002572656 0.003637427 0.002652179 0.002749322 0.002899605 0.002632316 0.003308516 0.002231822 0.002190906 0.0028107 0.003075113 0.004153868 0.003735241 0.003848774 0.004356775 0.003793578 0.002779214 0.007800829 0.000986011 0.008699435 0.000605617 0.004299065 0.002475888 0.001808358 0.001757649 0.001408672 0.001634342 0.002638617 0.001935297 0.002329797 0.003683566 0.00330959 0.001795425 0.002936784 0.004252008 0.001248222 0.001775659 0.002285347 0.002356718 0.00182944 0.001777507 0.001292196 0.001479483 0.001804258 0.003133948 0.003464207 0.001881819 0.001662812 0.003427258 0.00292409 0.002169337 PE 16:0/20:5 0.00698463 0.005378384 0.004125696 0.008435046 0.007900752 0.008414519 0.008186934 0.006933089 0.007571116 0.008584153 0.00972429 0.007027769 0.008340769 0.008706621 0.009321941 0.010026179 0.009726597 0.00931141 0.003607158 0.005875091 0.004235725 0.005334477 0.007218097 0.003924844 0.004849164 0.011983823 0.011827321 0.008498168 0.017158879 0.013015861 0.005539358 0.002701271 0.00175035 0.00757378 0.010261289 0.004643321 0.009064485 0.007264274 0.008318538 0.0121214 0.012266445 0.015417585 0.042205191 0.041451164 0.010923516 0.009706326 0.01189136 0.037290825 0.039363413 0.009111315 0.00809227 0.009824647 0.008547366 0.008340603 0.009024305 0.005976153 0.005670304 0.006319374 PE 16:0/20:4 0.042539002 0.032406038 0.025652911 0.0381715 0.033955481 0.039404366 0.043004403 0.045784551 0.040777043 0.044135505 0.048179436 0.0403446 0.050274174 0.05537411 0.05035209 0.04485787 0.044127281 0.058685354 0.032746231 0.040771106 0.035309222 0.029502573 0.037506641 0.043420795 0.095176136 0.097994302 0.105566172 0.06007326 0.048224299 0.054198539 0.032537006 0.022962829 0.035126882 0.043050958 0.053871768 0.048591271 0.041202205 0.038957082 0.041830364 0.044657789 0.047364595 0.05444842 0.036585803 0.02197342 0.057685982 0.053406529 0.058698608 0.050881556 0.02664064 0.044450833 0.043761825 0.041538713 0.035338396 0.03524423 0.033423354 0.03763827 0.045553323 0.037131908 PE 18:1/18:2 0.006037794 0.00740871 0.009730414 0.024255617 0.01704432 0.021730035 0.02727445 0.026293414 0.028515127 0.027453094 0.028951863 0.030870126 0.032062221 0.030438347 0.024291481 0.029930002 0.032970302 0.026056297 0.01161054 0.015194201 0.014303344 0.015437393 0.014419029 0.016194401 0.029968435 0.017141418 0.022799361 0.017582418 0.025383178 0.025539009 0.007663675 0.007978267 0.007672766 0.019266632 0.020229398 0.017334135 0.025545367 0.025971388 0.020321001 0.016040349 0.016564178 0.01955599 0.011549636 0.011503056 0.010408024 0.007700476 0.009269488 0.010276735 0.012772569 0.010018024 0.009730283 0.00775142 0.012255286 0.014544225 0.01066205 0.010630657 0.011106334 0.011020432 PE 16:0/20:3 0.004459734 0.002567599 0.002501601 0.010611832 0.007146186 0.006830659 0.00561127 0.006279024 0.008270621 0.010082331 0.007867835 0.006455136 0.008570331 0 0 0.028667446 0.026605103 0.006948346 0.005298013 0.002583014 0.006405411 0.006672384 0.00875441 0.007142509 0.000780083 0 0.002103992 0.002075702 0.010616822 0.006446341 0.001082326 0.00237728 0.00368053 0.004956175 0.007116937 0.006174851 0.004674577 0.004872849 0.005234737 0.006434367 0.004611109 0.008520245 0.006432406 0.009265554 0.005744051 0.005806408 0.005185044 0.005289004 0.007892601 0.006236386 0.007016561 0.006750041 0.003586064 0.004624808 0.003542875 0.00254479 0.003253809 0.004284069 PE 18:0/18:3 0.002264173 0.001329043 0.002098231 0.006957941 0.005370736 0.006787968 0.000754302 0.001643883 0.001518338 0.001558915 0.001153654 0.001337879 0.001300854 0.001079621 0.000633484 0.000868936 0.000779558 0.001604066 0.003336621 0.003722579 0.002902128 0.002487136 0.004523112 0.003627829 0 0 0 0.003750916 0.001962617 0.001732711 0.001969699 0.001607803 0.000815231 0.00239172 0.001114083 0.002283372 0.001535719 0.001330712 0.003404659 0.003025793 0.001540021 0.001606675 0.003921616 0.008089431 0.00407484 0.003881288 0.003742036 0.004853833 0.009510958 0.006369075 0.006405362 0.007973949 0.010427438 0.009903469 0.010478221 0.007757506 0.005292862 0.007346955 PE 18:1/18:1 0.12994979 0.161747415 0.163117129 0.150820182 0.158458909 0.139174686 0.105326291 0.115115442 0.106160214 0.09677418 0.100336991 0.109841428 0.107894346 0.08636968 0.104786783 0.101004475 0.100126732 0.093114095 0.120614344 0.104460131 0.102265456 0.120926244 0.11157582 0.125877874 0.072586863 0.06462163 0.0772197 0.087912088 0.059065421 0.073496504 0.135122689 0.142150844 0.134273407 0.102312461 0.090885703 0.091891802 0.103380079 0.122142665 0.111706085 0.121213999 0.100280427 0.103054386 0.079627925 0.135684432 0.077323763 0.076073254 0.075819035 0.064940934 0.095358553 0.078949987 0.081411664 0.075659798 0.119767567 0.117852877 0.118987139 0.100560264 0.11106334 0.109707909 PE 18:0/18:2 0.005845682 0.006277609 0.01638248 0.005830677 0.007590049 0.005848752 0.016051911 0.011598753 0.010081106 0.015751111 0.015205253 0.015877552 0.016222412 0.01595053 0.019120186 0.017452979 0.014589895 0.014710462 0.005264196 0.006964009 0.007462614 0.009176672 0.008059207 0.00876902 0.013131897 0.013167036 0.013586757 0.025592186 0.031514019 0.023855569 0.007663675 0.011096676 0.012348358 0.010297683 0.010261289 0.009815715 0.011611531 0.013692836 0.00825912 0.00672601 0.013878095 0.013794682 0.01054532 0.014658508 0.013157313 0.012171721 0.014919229 0.009205545 0.01648234 0.009973794 0.011319399 0.012721231 0.00950481 0.011880973 0.011397364 0.014735157 0.013058619 0.01906237 PE 18:0/18:1 0.096741924 0.106323476 0.125610805 0.110394154 0.127388535 0.123378786 0.107625991 0.096801622 0.109863477 0.118234564 0.109177254 0.11765006 0.086468519 0.080100913 0.095941145 0.073822388 0.07581024 0.08450691 0.139213752 0.138647083 0.147870322 0.136363636 0.114150646 0.111734293 0.063551154 0.088892664 0.07404294 0.096703297 0.106542056 0.074317694 0.117644133 0.1231164 0.120486405 0.100983728 0.087953906 0.089803352 0.137090974 0.141428349 0.144386056 0.107543247 0.116396924 0.127397942 0.087758103 0.106711646 0.080760375 0.091287905 0.09611897 0.073644358 0.107558472 0.097526455 0.088990527 0.101250612 0.09765931 0.099832072 0.103111046 0.103433415 0.105857246 0.101765255 PE 16:1/22:6 0.003169842 0.001470431 0.00114642 0.003245744 0.002272576 0.002467576 0.002285902 0.003710729 0.002818595 0.002445674 0.003956018 0.002592466 0.004208645 0.003426926 0.002708126 0.003750534 0.003604562 0.003411575 0.001752853 0.002532367 0.002770841 0.005471698 0.004385788 0.003882413 0.000975857 0.005885726 0 0.003125763 0.002986916 0.003239594 0.000887373 0.000534584 0.000779265 0.001249009 0.001964304 0.001615068 0.001663071 0.00281571 0.001039817 0.000993408 0.002247356 0.002199035 0.009971425 0.010699851 0.003534801 0.003198182 0.003301797 0.008067405 0.012000737 0.002631666 0.003300473 0.004153871 0.002663436 0.003093837 0.001838284 0.002372401 0.001778749 0.002228907 PE 18:1/20:5 0.000904297 0 0 0.011311514 0.009853747 0.012039037 0.019501462 0.020494037 0.019174674 0.017856658 0.02112823 0.015148747 0.013544184 0.015184347 0.015786061 0.016338959 0.014875972 0.015258192 0.010426941 0.009876231 0.010572037 0.007813036 0.009097721 0.00568572 0.015872729 0.015230074 0.00945697 0.018608059 0.021420561 0.02709927 0 0 0 0.022854212 0.026166287 0.016916445 0.022998322 0.014399977 0.019192056 0.017954254 0.02408521 0.024992717 0.012386566 0.008232861 0.002263254 0.001595986 0.002103367 0.00746486 0.00722036 0.003361456 0.003104889 0.003030101 0.00217601 0.003333052 0.002774138 0.000857841 0.001908901 0.002427474 PE 16:0/22:6 0.045146231 0.022056466 0.022857628 0.026937728 0.024368051 0.023651158 0.027780384 0.034839863 0.032671012 0.031664188 0.036333483 0.027850788 0.038872573 0.043811717 0.045657098 0.042332758 0.044413357 0.043114174 0.030153586 0.034440189 0.031439718 0.030017153 0.029524678 0.02432696 0.040660691 0.032159121 0.028346472 0.017582418 0.020186916 0.032272766 0.010621585 0.006277316 0.007432992 0.022787775 0.023894145 0.01782144 0.020601103 0.020699967 0.021984708 0.016678317 0.021936343 0.0219092 0.046150719 0.032128238 0.039275562 0.03632886 0.036931207 0.049207821 0.042326251 0.030518482 0.029337536 0.027964455 0.02506763 0.028705707 0.028075617 0.026597164 0.027158457 0.025515776 PE 18:1/20:4 0.114169193 0.082570359 0.066166818 0.106507036 0.087884773 0.094348483 0.124643776 0.120347963 0.116447056 0.123498431 0.137466098 0.109320852 0.111720387 0.106569041 0.094580278 0.117343434 0.111569787 0.114240823 0.08961533 0.080402646 0.078081058 0.09348199 0.090118931 0.094054816 0.171678473 0.167166751 0.146619683 0.111355311 0.10953271 0.103880533 0.090081793 0.078972691 0.092312967 0.102976828 0.133396758 0.119041652 0.092892245 0.101571269 0.090315558 0.085670045 0.083447641 0.080820605 0.055954758 0.057084994 0.070205068 0.069242186 0.075574458 0.063267198 0.050791501 0.06590223 0.068454252 0.058970137 0.072069437 0.070647936 0.065175539 0.057052558 0.057700876 0.057087826 PE 18:0/20:5 0.015506152 0.012611774 0.015037913 0.011777968 0.008433387 0.012252495 0.019455468 0.015479539 0.012179621 0.022513157 0.015337857 0.020666847 0.016681537 0.022776521 0.015718017 0.021983327 0.020311423 0.022769917 0.012963224 0.012155361 0.012023101 0.010634648 0.010385134 0.009051892 0.025661414 0.012256872 0.011045349 0.006007326 0.013457944 0.009402625 0.02137762 0.024380287 0.028233382 0.019133759 0.016711242 0.016498755 0.018653362 0.021599966 0.02287598 0.017954254 0.017280466 0.015011859 0.015136479 0.010671165 0.014556505 0.013879488 0.014821398 0.011347926 0.013170933 0.012384312 0.012712932 0.011126441 0.011106353 0.010844378 0.010127276 0.013996347 0.010238652 0.011218999 PE 18:1/20:3 0.005132125 0.012668329 0.008350465 0.007502138 0.009010409 0.008026025 0.014258144 0.013735365 0.011850442 0.01320016 0.009856894 0.011817064 0.012855497 0.016856018 0.018711925 0.015819083 0.017450659 0.018075089 0.007214316 0.007153936 0.007669909 0.009519726 0.008007711 0.005890802 0.012619874 0.013349069 0.004276407 0.014749695 0.010542056 0.011784076 0.005868762 0.008180761 0.008871636 0.00837102 0.010774354 0.009258795 0.009963442 0.011121411 0.011586535 0.005605008 0.011639692 0.006207607 0.007891056 0.003614427 0.008272415 0.005899558 0.007899854 0.006494093 0.005278332 0.007695965 0.007481072 0.005822837 0.011054129 0.01137065 0.009542367 0.006813471 0.006507618 0.008190862 PE 16:0/22:4 0.014408372 0.010971678 0.011322664 0.026315789 0.020772765 0.016180124 0.011130551 0.010552249 0.011973885 0.012714265 0.010608316 0.00827715 0.00849381 0.011632046 0.010886938 0.012254219 0.011371536 0.011032847 0.01375229 0.015827293 0.011193922 0.013893654 0.016049753 0.013082813 0.014035468 0.018992085 0.018229714 0.00996337 0.007738318 0.006487401 0.01236944 0.006479811 0.007612823 0.009899063 0.005585073 0.006648233 0.010113269 0.008871415 0.01111119 0.012030262 0.010744331 0.008844825 0.008680161 0.014486393 0.013623711 0.012451173 0.012913694 0.014661922 0.014988472 0.013423709 0.014448737 0.013277553 0.021586015 0.019934519 0.018215728 0.018347118 0.014446911 0.012658605 PE 18:0/20:4 0.194856074 0.219999106 0.223268782 0.136049133 0.152244835 0.161801245 0.207433001 0.184882377 0.204090954 0.177351845 0.175037218 0.19261293 0.17599787 0.19572484 0.183036653 0.149278672 0.170215444 0.150234507 0.180357898 0.168402393 0.15478015 0.151801029 0.181096138 0.170430156 0.146378488 0.160492217 0.180830943 0.251037851 0.233271028 0.219257726 0.257472587 0.291996462 0.270345119 0.261096085 0.199362188 0.262448555 0.186533621 0.161356889 0.161023133 0.190479142 0.17817683 0.180953764 0.121713555 0.079173157 0.189995532 0.18971738 0.180498219 0.141263269 0.115276789 0.161880646 0.168201875 0.175426884 0.121856537 0.116577068 0.118151555 0.144478421 0.122777052 0.142967773 PE 18:1/20:2 0.013338035 0.019059049 0.022326878 0.008979243 0.014469917 0.007001426 0.013016306 0.00885168 0.014689611 0.011985422 0.009326478 0.011608833 0.020201495 0.020756584 0.022522354 0.015967619 0.016306353 0.019874773 0.014879527 0.01221867 0.014648836 0.010634648 0.01613558 0.012375634 0.018613561 0.010739933 0.010214505 0.023980464 0.024485981 0.023527093 0.014453422 0.014458077 0.010550053 0.010231246 0.010554469 0.00821457 0.006382596 0.009899984 0.010398173 0.01157457 0.009132682 0.007441014 0.014682146 0.01873191 0.020987879 0.018568084 0.018538977 0.019448805 0.01501337 0.023220584 0.022198736 0.021177326 0.024023146 0.023442994 0.023062114 0.023149383 0.023644344 0.018963087 PE 18:0/20:3 0.007382575 0.010406127 0.010508848 0.025382881 0.027386316 0.023224242 0.003012608 0.002572656 0.002221958 0.00334863 0.002895186 0.000853744 0.001974237 0.001455747 0.001007042 0.001596762 0.001544812 0.001619716 0.008679724 0.008166883 0.008360892 0.006415094 0.007166601 0.005791797 0 0.003792349 0.000701331 0.002739927 0.000385047 0.00209814 0.004013346 0.002952364 0.002523621 0.001514756 0.001678454 0.002165027 0.003438511 0.003053567 0.002014275 0.004010087 0.003697841 0.00168782 0.013701742 0.028571183 0.014311033 0.010991809 0.011079362 0.012720389 0.039587493 0.015856342 0.015084383 0.018024834 0.038471849 0.038912181 0.042280542 0.021918033 0.019132396 0.02501936 PE 18:0/20:2 0.004967458 0.004677102 0.005095199 0.006102775 0.006169689 0.005336453 0.002042134 0.002428761 0.001715845 0.002121744 0.003292998 0.002066685 0.002372145 0.001741324 0.002987104 0.002569673 0.002281459 0.003192483 0.003578977 0.004311354 0.005997731 0.005308748 0.004497364 0.004236003 0.00490639 0.003886399 0.005515344 0.001489621 0.001196262 0.001281056 0.002769679 0.003279999 0.002639312 0.00231864 0.002198848 0.00064185 0.002959067 0.003143566 0.003648273 0.002433394 0.002820387 0.002466814 0.004029221 0.009093439 0.002719833 0.003760192 0.00261698 0.005931718 0.008340761 0.003330495 0.0035694 0.003289718 0.005918746 0.004688599 0.00528089 0.004309726 0.003748388 0.00330613 PE 18:0/20:1 0.001336548 0.002194336 0.00137995 0.00203685 0.003568654 0.00234377 0.001545399 0.001172957 0.001913353 0.002117694 0.001953698 0.001582549 0.000580028 0.002430889 0.001068281 0.000468631 0.001251584 0.001369325 0.00268846 0.002905891 0.002356251 0.001989708 0.001072844 0.002786286 0.000978868 0.003944043 0.000781972 0.000781441 0.000583178 0.003871911 0.000873928 0.000785677 0.001354723 0.001474894 0.001861691 0.000744881 0.00165558 0.001639283 0.002626281 0.001011636 0.002712944 0.001809538 0.00202298 0.002134233 0.001183176 0.002291513 0.002543606 0.000763223 0.004157932 0.003339341 0.002148975 0.00268889 0.002193418 0.003428737 0.003576299 0.001580233 0.00431672 0.003986219 PE 18:1/22:6 0.040206218 0.023866227 0.021689978 0.030863718 0.023214008 0.028944919 0.018075647 0.029127695 0.027445296 0.024740178 0.025990375 0.023165609 0.021578869 0.027373616 0.023066701 0.021537719 0.026605103 0.025743309 0.029139073 0.027982653 0.027708411 0.034819897 0.032185333 0.03380316 0.013884873 0.003944043 0.010409998 0.006202686 0.012261682 0.020160214 0.008739278 0.008018766 0.008871636 0.013353769 0.015391934 0.01447992 0.023447801 0.020121397 0.024242597 0.012941645 0.012893198 0.013470101 0.057628618 0.066838209 0.029702144 0.03229232 0.034485432 0.056572257 0.050791501 0.031181928 0.029093057 0.029040011 0.032379023 0.035084754 0.037601273 0.022944158 0.027852603 0.028990687 PE 18:1/22:5 0.023602285 0.014308425 0.012737997 0.012011195 0.006924255 0.007812567 0.008186934 0.008372032 0.009093569 0.00753138 0.008530854 0.00827715 0.008799893 0.010378292 0.008369334 0.011437271 0.010870902 0.007981208 0.00463858 0.00683739 0.009120973 0.007658662 0.009441031 0.006371683 0.025209628 0.021570882 0.019647038 0.007179487 0.003925234 0.00903309 0.006722522 0.006803801 0.007313105 0.005188703 0.006369329 0.003821864 0.005273882 0.005528563 0.006120067 0.003827811 0.004852856 0.00303483 0.012338741 0.013970046 0.016004792 0.016580864 0.013207187 0.014963195 0.010905234 0.014728485 0.014913248 0.013351729 0.017059915 0.019296614 0.019385545 0.014776202 0.015444746 0.012906813 PE 18:0/22:6 0.016329488 0.027372639 0.021689978 0.013566042 0.013670965 0.013106328 0.009336785 0.012994092 0.011233232 0.014050477 0.010917725 0.014419941 0.014003309 0.018458037 0.015718017 0.016041887 0.01344559 0.012910778 0.022150204 0.025070431 0.025220873 0.022727273 0.021371061 0.023195473 0.01554142 0.011650097 0.009359223 0.006153846 0.009457944 0.013015861 0.01236944 0.00785677 0.005580738 0.006171966 0.006274045 0.006613425 0.00846518 0.0093857 0.009744573 0.010389771 0.010207115 0.006840539 0.087758103 0.051634668 0.084442459 0.084146334 0.07826481 0.093394436 0.057264927 0.082267214 0.08312302 0.083077425 0.071547195 0.070010031 0.073865611 0.089478113 0.088503599 0.088362026 PE 18:1/22:4 0.017427269 0.014534645 0.011393431 0.008007463 0.009409885 0.0076418 0.007543018 0.010203414 0.006830463 0.007045484 0.007823633 0.008745668 0.00507333 0.005697613 0.008301291 0.006609852 0.006150642 0.00534428 0.010990559 0.013421544 0.013128673 0.012435678 0.01124341 0.011173429 0.003794998 0.004823868 0.011754011 0.003677656 0.007962617 0.005666211 0.013108918 0.00923373 0.012408301 0.005627185 0.007329492 0.004559783 0.008315354 0.007457131 0.008318538 0.006334115 0.006724161 0.006329324 0.011621373 0.007487027 0.013869183 0.012637475 0.013989835 0.013122086 0.008888513 0.015524619 0.015646686 0.01513196 0.014727233 0.01417743 0.013686863 0.019003838 0.014316759 0.015190326 PE 18:0/22:5 0.019348385 0.019624599 0.021017695 0.004897769 0.009764975 0.007471034 0.006393168 0.008982493 0.006583579 0.009232014 0.008884465 0.007288057 0.014003309 0.011005169 0.012656066 0.011288735 0.010870902 0.014632215 0.014879527 0.010319395 0.009397366 0.010548885 0.009097721 0.008203277 0.016716062 0.007857747 0.022139572 0.012796093 0.009943925 0.008909911 0.011293837 0.011177673 0.009231297 0.005162129 0.005064679 0.006383696 0.006502457 0.005155706 0.005365457 0.008457638 0.007485218 0.005306895 0.028694748 0.01626492 0.040011979 0.043780934 0.046469731 0.026177222 0.019843542 0.047325763 0.04596214 0.047101933 0.035860638 0.035722658 0.037601273 0.045149506 0.051193258 0.043188181 PE 18:0/22:4 0.012926368 0.011933113 0.013870263 0.005519708 0.004838101 0.005122995 0.007924769 0.005361153 0.005118733 0.00650695 0.007173874 0.008428117 0.008759337 0.006674844 0.008516308 0.007730556 0.00709684 0.007573541 0.012478512 0.01194011 0.01164997 0.013181818 0.012650981 0.012326131 0.017589514 0.010861288 0.003953844 0.013763126 0.008811215 0.009402625 0.022453223 0.015308552 0.017263724 0.006025805 0.005980866 0.003949955 0.007611171 0.007817131 0.008633454 0.006206521 0.006768929 0.007514044 0.012362654 0.011732544 0.02238707 0.024032938 0.022378844 0.025373829 0.01750315 0.032508818 0.031293372 0.025924607 0.015319107 0.015931668 0.01644429 0.021384448 0.021041297 0.025813626 PE 18:1/22:2 0 0 0 0.001018425 0.000470494 0.000841025 0.000131543 0.000119476 6.13096E-05 0.00025388 0.000140118 0.000645514 0.000114016 0.000103086 0.000530738 0.000353516 0.000106563 0 0.000789066 0.000503308 0.000452594 0.00025729 0.000926938 0.000171137 0.000990916 0.000998146 0.000803965 0 0.00355514 0.000652846 0.000221171 0 0 0.00041988 0.000449298 0.000331367 0.000351342 0.000406285 0.000469403 0.00089589 0.00085507 0.001192834 0.00025347 0.001543303 0.000621045 0.000397444 0.000235528 0.000445214 0.001000891 0.000247686 0.000225655 0.000370881 0.000503093 0.001227966 0.000568197 0.000800378 0.000226031 0.000838943 PE 18:0/22:3 0 0.000372698 0.000817355 0.002927 0.003200249 0.001677781 0.000634717 0.000976737 0.000588407 0.000643811 0.000423891 0.000640308 0 0.000103086 9.86629E-05 0.000590431 0.000340431 0.000488262 0.001059603 0.001411794 0.002100588 0.002024014 0.001364658 0.001124415 0 0 0 0.001562882 0.001196262 0 0.000873928 0.000534584 0.000989068 0.000611217 0.000466889 0.000445536 0.00023298 0.000203143 0.000177066 0.001321506 0.000575717 0.00025155 0.001944069 0.004761864 0.001664302 0.001496625 0.001883247 0.004652984 0.008515046 0.002941274 0.003716088 0.004858546 0.007067679 0.006825579 0.007603813 0.002914195 0.003761403 0.003345843 PE 18:0/22:2 0 0 0 0.001527637 0.002898422 0.001746087 0.000804895 0.000619182 0.000987537 0.001036577 0.000769103 0.000167105 0.001216681 0.000773148 0.000325928 0.000118829 0.000570722 0.000495304 0.001341412 0.002791934 0.001962391 0.001620926 0.001356075 0.000883974 0 0 0 0 0 0.000640528 0 0 0 0.000525514 0.000348884 0 0.001063766 0.001137855 0.000653599 0.000142176 0.00128932 0.000908826 0.005260704 0.008691836 0.002243616 0.001959274 0.001540838 0.002902257 0.004780377 0.001800148 0.00208541 0.002381058 0.003359759 0.00408259 0.004996791 0.002072773 0.002611724 0.002338119 PE 18:0/22:1 0 0 0 0.00089015 0.001478062 0.000947754 0.001168248 0.00034491 0.000707735 0.000453502 0.000561357 0.000749629 0.000244867 0.000884593 0.000748477 0.000582261 0.000452001 0.000372456 0.000721432 0.000414675 0.000655743 0.001226415 0.001227334 0.000669699 0.001957737 0.000986011 0 0 0.000598131 0 0 0 0 0.000730803 0.000465423 0.001211301 0.000839027 0.002031425 0.001628057 0.00086946 0.001137108 0.00064835 0.006169371 0.004733178 0.002749289 0.002046215 0.001447899 0.003682218 0.006597916 0.002153986 0.003300473 0.002789028 0.001793032 0.003045995 0.0032922 0.001563815 0.001761395 0.00236294 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Q1 (m/z) Q3 (m/z) Retention time (min) pPE 16:0/16:1 674.5 364.2 41.5 pPE 16:0/16:0 676.5 364.2 45.9 pPE 16:0/18:2 700.5 364.2 42.3 pPE 16:0/18:1 702.5 364.2 45.8 pPE 16:0/20:5 722.5 364.2 38.4 pPE 16:0/20:4 724.5 364.2 41.3 pPE 16:0/20:3 726.5 364.2 43.2 pPE 16:0/20:2 728.5 364.2 47 pPE 16:0/20:1 730.5 364.2 51 pPE 16:0/22:6 748.5 364.2 40 pPE 16:0/22:5 750.5 364.2 41.4 pPE 16:0/22:4 752.5 364.2 44.4 pPE 16:0/22:3 754.5 364.2 47.5 pPE 18:0/16:1 702.5 392.2 45.8 pPE 18:0/16:0 704.5 392.2 50.7 pPE 18:0/18:2 728.5 392.2 46.6 pPE 18:0/18:1 730.5 392.2 50.3 pPE 18:0/20:5 750.3 392.2 42.6 pPE 18:0/20:4 752.3 392.2 45.5 pPE 18:0/20:3 754.3 392.2 47.7 pPE 18:0/20:2 756.3 392.2 51.5 pPE 18:0/20:1 758.3 392.2 55.9 pPE 18:0/22:6 776.5 392.2 44.2 pPE 18:0/22:5 778.5 392.2 45.6 pPE 18:0/22:4 780.5 392.2 48.4 pPE 18:1/16:1 700.5 390.2 42 pPE 18:1/16:0 702.5 390.2 45.9 pPE 18:1/18:2 726.5 390.2 42.4 pPE 18:1/18:1 728.5 390.2 45.7 pPE 18:1/20:5 748.5 390.2 38.4 pPE 18:1/20:4 750.5 390.2 41.3 pPE 18:1/20:3 752.5 390.2 44.4 pPE 18:1/20:2 754.5 390.2 46.5 pPE 18:1/20:1 756.5 390.2 50.5 pPE 18:1/22:6 774.5 390.2 40 pPE 18:1/22:5 776.5 390.2 41.6 pPE 18:1/22:4 778.5 390.2 44 pPE 18:1/22:3 780.5 390.2 48.6 PE 14:0/18:2 688.5 547.4 36.6 PE 16:0/16:1 690.5 549.4 40.3 PE 16:0/16:0 692.5 551.4 44.7 PE 16:1/18:2 714.5 573.4 36.8 PE 16:0/18:2 716.5 575.4 40.2 PE 16:0/18:1 718.5 577.4 44.6 PE 16:0/18:0 720.5 579.4 49.1 PE 16:0/20:5 738.5 597.4 37.3 PE 16:0/20:4 740.5 599.4 40.1 PE 18:1/18:2 742.5 601.4 41.1 PE 16:0/20:3 742.5 601.4 41.8 PE 18:0/18:3 742.5 601.4 42.5 PE 18:1/18:1 744.5 603.4 44.5 PE 18:0/18:2 744.5 603.4 45.3 PE 18:0/18:1 746.5 605.4 49.1 PE 16:1/22:6 762.5 621.4 34.9 PE 18:1/20:5 764.5 623.4 37.4 PE 16:0/22:6 764.5 623.4 38.4 PE 18:1/20:4 766.5 625.4 40.2 PE 18:0/20:5 766.5 625.4 41.7 PE 18:1/20:3 768.5 627.4 42.1 PE 16:0/22:4 768.5 627.4 43.4 PE 18:0/20:4 768.5 627.4 44.6 PE 18:1/20:2 770.5 629.4 46.4 PE 18:0/20:3 770.5 629.4 47.8 PE 18:0/20:2 772.5 631.4 48.6 PE 18:0/20:1 774.5 633.4 52.6 PE 18:1/22:6 790.5 649.4 38.9 PE 18:1/22:5 792.5 651.4 41.7 PE 18:0/22:6 792.5 651.4 43.2 PE 18:1/22:4 794.5 653.4 43.3 PE 18:0/22:5 794.5 653.4 46.1 PE 18:0/22:4 796.5 655.4 48.5 PE 18:1/22:2 798.5 657.4 49.5 PE 18:0/22:3 798.5 657.4 50.9 PE 18:0/22:2 800.5 659.4 54.3 PE 18:0/22:1 802.5 661.4 59.6 METABOLITES_END #END